Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0712300 | AES77703.1 | 71.739 | 46 | 10 | 1 | 67 | 109 | 8 | 53 | 9.85e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0712300 | G7JQU8 | 71.739 | 46 | 10 | 1 | 67 | 109 | 8 | 53 | 4.70e-11 | 64.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0712300 | Msa0830980 | 0.982290 | 1.033740e-154 | -8.615850e-47 |
| Msa0712300 | Msa1248300 | 0.800429 | 1.447579e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0712300 | MtrunA17_Chr7g0258451 | 90.625 | 32 | 3 | 0 | 76 | 107 | 531 | 562 | 6.53e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCCTTTGGGTATTGCTTC+TGG | 0.183197 | 5_1:-4156890 | Msa0712300:CDS |
| TGAATGTTGCCGAGAGAGAT+AGG | 0.336678 | 5_1:+4157283 | None:intergenic |
| AGGAGAGATGTATTTGCCTT+TGG | 0.340701 | 5_1:-4156903 | Msa0712300:CDS |
| TTGCTTCTGGAATTGTTAAC+AGG | 0.376041 | 5_1:-4156877 | Msa0712300:CDS |
| GGAGAGGGAAGAAGAAGAAA+GGG | 0.378209 | 5_1:-4156640 | Msa0712300:CDS |
| GGAAGAATAGAAGCAAGATC+TGG | 0.379033 | 5_1:-4156668 | Msa0712300:CDS |
| GGAGAGATGTATTTGCCTTT+GGG | 0.380769 | 5_1:-4156902 | Msa0712300:CDS |
| ATCGTCTCTCTTGCTCTCAT+TGG | 0.384369 | 5_1:-4157226 | Msa0712300:CDS |
| GGCTCGTGATAATGTGGGTT+TGG | 0.413016 | 5_1:-4156701 | Msa0712300:CDS |
| AAGAGCAAAATCTGGCGAAA+TGG | 0.420785 | 5_1:-4156734 | Msa0712300:CDS |
| TAGAAGCAAGATCTGGGTAT+TGG | 0.442915 | 5_1:-4156661 | Msa0712300:CDS |
| TGGAGAGGGAAGAAGAAGAA+AGG | 0.449111 | 5_1:-4156641 | Msa0712300:CDS |
| CCCACAGGCTCGTGATAATG+TGG | 0.462403 | 5_1:-4156707 | Msa0712300:CDS |
| AGAGCAAAATCTGGCGAAAT+GGG | 0.466130 | 5_1:-4156733 | Msa0712300:CDS |
| TGAGAAGCATACCTTTCAAA+AGG | 0.470235 | 5_1:+4156847 | None:intergenic |
| TGTGGGTTTGGAGAGGGAAG+AGG | 0.470286 | 5_1:-4156689 | Msa0712300:CDS |
| AGAAGAAGAAAGGGGTAGAA+TGG | 0.475217 | 5_1:-4156631 | Msa0712300:CDS |
| GCCAAGGGAAGAGCAAAATC+TGG | 0.491444 | 5_1:-4156742 | Msa0712300:CDS |
| GAATGTTGCCGAGAGAGATA+GGG | 0.525707 | 5_1:+4157284 | None:intergenic |
| TGAGAGGCAAACGACGGAGG+AGG | 0.532318 | 5_1:+4157253 | None:intergenic |
| TTCATTACAGTGTCGGGCCA+AGG | 0.537815 | 5_1:-4156758 | Msa0712300:intron |
| GAAGAATAGAAGCAAGATCT+GGG | 0.541251 | 5_1:-4156667 | Msa0712300:CDS |
| TTCTGGAATTGTTAACAGGA+AGG | 0.547060 | 5_1:-4156873 | Msa0712300:CDS |
| GCGAAAACCCCTATCTCTCT+CGG | 0.548196 | 5_1:-4157292 | Msa0712300:CDS |
| CCACAGGCTCGTGATAATGT+GGG | 0.551761 | 5_1:-4156706 | Msa0712300:CDS |
| TGGCGAAATGGGTTTCCCAC+AGG | 0.558172 | 5_1:-4156722 | Msa0712300:CDS |
| TGATAATGTGGGTTTGGAGA+GGG | 0.569156 | 5_1:-4156695 | Msa0712300:CDS |
| GATTATGTTGAAGATTATGA+AGG | 0.571896 | 5_1:-4156923 | Msa0712300:intron |
| TCATTACAGTGTCGGGCCAA+GGG | 0.574278 | 5_1:-4156757 | Msa0712300:intron |
| AAGAGAGACGATGCGGTGAG+AGG | 0.574533 | 5_1:+4157237 | None:intergenic |
| GAGAGGGAAGAAGAAGAAAG+GGG | 0.583757 | 5_1:-4156639 | Msa0712300:CDS |
| CCCACATTATCACGAGCCTG+TGG | 0.598288 | 5_1:+4156706 | None:intergenic |
| AATGTTGCCGAGAGAGATAG+GGG | 0.600552 | 5_1:+4157285 | None:intergenic |
| CAAGATCTGGGTATTGGAGA+GGG | 0.604886 | 5_1:-4156655 | Msa0712300:CDS |
| GTGATAATGTGGGTTTGGAG+AGG | 0.608427 | 5_1:-4156696 | Msa0712300:CDS |
| GCAAGATCTGGGTATTGGAG+AGG | 0.632518 | 5_1:-4156656 | Msa0712300:CDS |
| CGGTGAGAGGCAAACGACGG+AGG | 0.639021 | 5_1:+4157250 | None:intergenic |
| GATTCAGATTCAGACTCCGA+AGG | 0.644186 | 5_1:+4157058 | None:intergenic |
| CCACATTATCACGAGCCTGT+GGG | 0.673056 | 5_1:+4156707 | None:intergenic |
| ATGCGGTGAGAGGCAAACGA+CGG | 0.684988 | 5_1:+4157247 | None:intergenic |
| ATTCCAGAAGCAATACCCAA+AGG | 0.688016 | 5_1:+4156887 | None:intergenic |
| TGAGAGCAAGAGAGACGATG+CGG | 0.700987 | 5_1:+4157230 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGATTGAATCAAAATGATTA+AGG | - | chr5_1:4156788-4156807 | Msa0712300:intron | 20.0% |
| !! | GATTGAATCAAAATGATTAA+GGG | - | chr5_1:4156789-4156808 | Msa0712300:intron | 20.0% |
| ! | ATCACAAACATAGGAATAAT+GGG | + | chr5_1:4156772-4156791 | None:intergenic | 25.0% |
| ! | AATCACAAACATAGGAATAA+TGG | + | chr5_1:4156773-4156792 | None:intergenic | 25.0% |
| ! | TTTGATTCAATCACAAACAT+AGG | + | chr5_1:4156781-4156800 | None:intergenic | 25.0% |
| ! | AATAAACAAATGACCCTTTA+TGG | + | chr5_1:4156831-4156850 | None:intergenic | 25.0% |
| ! | TATGAACATGATCTATCAAA+GGG | - | chr5_1:4156946-4156965 | Msa0712300:intron | 25.0% |
| !! | ATCATGGTGATTATATTTTC+TGG | - | chr5_1:4156999-4157018 | Msa0712300:intron | 25.0% |
| !! | TCATGGTGATTATATTTTCT+GGG | - | chr5_1:4157000-4157019 | Msa0712300:intron | 25.0% |
| ! | GATTATGTTGAAGATTATGA+AGG | - | chr5_1:4157031-4157050 | Msa0712300:intron | 25.0% |
| AACTTCTCTGTTATCCATAA+AGG | - | chr5_1:4156814-4156833 | Msa0712300:intron | 30.0% | |
| ACTTCTCTGTTATCCATAAA+GGG | - | chr5_1:4156815-4156834 | Msa0712300:intron | 30.0% | |
| AGAATTGAAGCAAAAAGAGA+AGG | + | chr5_1:4156875-4156894 | None:intergenic | 30.0% | |
| ! | TTTTGCTTCAATTCTTCCTT+CGG | - | chr5_1:4156880-4156899 | Msa0712300:CDS | 30.0% |
| GTATGAACATGATCTATCAA+AGG | - | chr5_1:4156945-4156964 | Msa0712300:intron | 30.0% | |
| ATGAACATGATCTATCAAAG+GGG | - | chr5_1:4156947-4156966 | Msa0712300:intron | 30.0% | |
| CTTTCAATTCATTACAGTGT+CGG | - | chr5_1:4157189-4157208 | Msa0712300:intron | 30.0% | |
| TTTCAATTCATTACAGTGTC+GGG | - | chr5_1:4157190-4157209 | Msa0712300:intron | 30.0% | |
| ! | CAAACATAGGAATAATGGGA+AGG | + | chr5_1:4156768-4156787 | None:intergenic | 35.0% |
| TTGAAGCAAAAAGAGAAGGA+AGG | + | chr5_1:4156871-4156890 | None:intergenic | 35.0% | |
| ! | CACCATGATTTTTCTCTGTT+TGG | + | chr5_1:4156988-4157007 | None:intergenic | 35.0% |
| !! | TTGCTTCTGGAATTGTTAAC+AGG | - | chr5_1:4157077-4157096 | Msa0712300:CDS | 35.0% |
| !! | TTCTGGAATTGTTAACAGGA+AGG | - | chr5_1:4157081-4157100 | Msa0712300:CDS | 35.0% |
| ! | TGAGAAGCATACCTTTCAAA+AGG | + | chr5_1:4157110-4157129 | None:intergenic | 35.0% |
| GAAGAATAGAAGCAAGATCT+GGG | - | chr5_1:4157287-4157306 | Msa0712300:CDS | 35.0% | |
| ! | CATAGGAATAATGGGAAGGA+AGG | + | chr5_1:4156764-4156783 | None:intergenic | 40.0% |
| TCAAAGGGGTCAAAGAGAAA+AGG | - | chr5_1:4156961-4156980 | Msa0712300:intron | 40.0% | |
| CAAAGGGGTCAAAGAGAAAA+GGG | - | chr5_1:4156962-4156981 | Msa0712300:intron | 40.0% | |
| GGCCAAACAGAGAAAAATCA+TGG | - | chr5_1:4156983-4157002 | Msa0712300:intron | 40.0% | |
| AGGAGAGATGTATTTGCCTT+TGG | - | chr5_1:4157051-4157070 | Msa0712300:intron | 40.0% | |
| GGAGAGATGTATTTGCCTTT+GGG | - | chr5_1:4157052-4157071 | Msa0712300:intron | 40.0% | |
| ATTCCAGAAGCAATACCCAA+AGG | + | chr5_1:4157070-4157089 | None:intergenic | 40.0% | |
| ! | CAGGAAGGAAACCTTTTGAA+AGG | - | chr5_1:4157096-4157115 | Msa0712300:CDS | 40.0% |
| AAGAGCAAAATCTGGCGAAA+TGG | - | chr5_1:4157220-4157239 | Msa0712300:intron | 40.0% | |
| AGAGCAAAATCTGGCGAAAT+GGG | - | chr5_1:4157221-4157240 | Msa0712300:intron | 40.0% | |
| ! | TGATAATGTGGGTTTGGAGA+GGG | - | chr5_1:4157259-4157278 | Msa0712300:CDS | 40.0% |
| GGAAGAATAGAAGCAAGATC+TGG | - | chr5_1:4157286-4157305 | Msa0712300:CDS | 40.0% | |
| TAGAAGCAAGATCTGGGTAT+TGG | - | chr5_1:4157293-4157312 | Msa0712300:CDS | 40.0% | |
| AGAAGAAGAAAGGGGTAGAA+TGG | - | chr5_1:4157323-4157342 | Msa0712300:CDS | 40.0% | |
| AATGTTGCCGAGAGAGATAG+GGG | + | chr5_1:4156672-4156691 | None:intergenic | 45.0% | |
| GAATGTTGCCGAGAGAGATA+GGG | + | chr5_1:4156673-4156692 | None:intergenic | 45.0% | |
| TGAATGTTGCCGAGAGAGAT+AGG | + | chr5_1:4156674-4156693 | None:intergenic | 45.0% | |
| ATCGTCTCTCTTGCTCTCAT+TGG | - | chr5_1:4156728-4156747 | Msa0712300:CDS | 45.0% | |
| ! | GGAATAATGGGAAGGAAGGA+AGG | + | chr5_1:4156760-4156779 | None:intergenic | 45.0% |
| GATTCAGATTCAGACTCCGA+AGG | + | chr5_1:4156899-4156918 | None:intergenic | 45.0% | |
| ! | TTGCCTTTGGGTATTGCTTC+TGG | - | chr5_1:4157064-4157083 | Msa0712300:CDS | 45.0% |
| CAGTGTCTCTGCTCTTACAA+AGG | - | chr5_1:4157128-4157147 | Msa0712300:intron | 45.0% | |
| ! | GTGATAATGTGGGTTTGGAG+AGG | - | chr5_1:4157258-4157277 | Msa0712300:CDS | 45.0% |
| !! | CAAGATCTGGGTATTGGAGA+GGG | - | chr5_1:4157299-4157318 | Msa0712300:CDS | 45.0% |
| TGGAGAGGGAAGAAGAAGAA+AGG | - | chr5_1:4157313-4157332 | Msa0712300:CDS | 45.0% | |
| GGAGAGGGAAGAAGAAGAAA+GGG | - | chr5_1:4157314-4157333 | Msa0712300:CDS | 45.0% | |
| GAGAGGGAAGAAGAAGAAAG+GGG | - | chr5_1:4157315-4157334 | Msa0712300:CDS | 45.0% | |
| GCGAAAACCCCTATCTCTCT+CGG | - | chr5_1:4156662-4156681 | Msa0712300:CDS | 50.0% | |
| TGAGAGCAAGAGAGACGATG+CGG | + | chr5_1:4156727-4156746 | None:intergenic | 50.0% | |
| ! | TTCATTACAGTGTCGGGCCA+AGG | - | chr5_1:4157196-4157215 | Msa0712300:intron | 50.0% |
| ! | TCATTACAGTGTCGGGCCAA+GGG | - | chr5_1:4157197-4157216 | Msa0712300:intron | 50.0% |
| GCCAAGGGAAGAGCAAAATC+TGG | - | chr5_1:4157212-4157231 | Msa0712300:intron | 50.0% | |
| ! | GCCAGATTTTGCTCTTCCCT+TGG | + | chr5_1:4157216-4157235 | None:intergenic | 50.0% |
| CCACATTATCACGAGCCTGT+GGG | + | chr5_1:4157250-4157269 | None:intergenic | 50.0% | |
| ! | CCACAGGCTCGTGATAATGT+GGG | - | chr5_1:4157248-4157267 | Msa0712300:CDS | 50.0% |
| ! | GGCTCGTGATAATGTGGGTT+TGG | - | chr5_1:4157253-4157272 | Msa0712300:CDS | 50.0% |
| !! | GCAAGATCTGGGTATTGGAG+AGG | - | chr5_1:4157298-4157317 | Msa0712300:CDS | 50.0% |
| ATGCGGTGAGAGGCAAACGA+CGG | + | chr5_1:4156710-4156729 | None:intergenic | 55.0% | |
| AAGAGAGACGATGCGGTGAG+AGG | + | chr5_1:4156720-4156739 | None:intergenic | 55.0% | |
| ! | TGGCGAAATGGGTTTCCCAC+AGG | - | chr5_1:4157232-4157251 | Msa0712300:CDS | 55.0% |
| ! | CCCACAGGCTCGTGATAATG+TGG | - | chr5_1:4157247-4157266 | Msa0712300:CDS | 55.0% |
| CCCACATTATCACGAGCCTG+TGG | + | chr5_1:4157251-4157270 | None:intergenic | 55.0% | |
| TGTGGGTTTGGAGAGGGAAG+AGG | - | chr5_1:4157265-4157284 | Msa0712300:CDS | 55.0% | |
| TGAGAGGCAAACGACGGAGG+AGG | + | chr5_1:4156704-4156723 | None:intergenic | 60.0% | |
| CGGTGAGAGGCAAACGACGG+AGG | + | chr5_1:4156707-4156726 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 4156623 | 4157353 | 4156623 | ID=Msa0712300;Name=Msa0712300 |
| chr5_1 | mRNA | 4156623 | 4157353 | 4156623 | ID=Msa0712300-mRNA-1;Parent=Msa0712300;Name=Msa0712300-mRNA-1;_AED=0.51;_eAED=0.59;_QI=0|0|0|0.5|1|1|4|0|138 |
| chr5_1 | exon | 4157223 | 4157353 | 4157223 | ID=Msa0712300-mRNA-1:exon:1674;Parent=Msa0712300-mRNA-1 |
| chr5_1 | exon | 4157054 | 4157117 | 4157054 | ID=Msa0712300-mRNA-1:exon:1673;Parent=Msa0712300-mRNA-1 |
| chr5_1 | exon | 4156859 | 4156932 | 4156859 | ID=Msa0712300-mRNA-1:exon:1672;Parent=Msa0712300-mRNA-1 |
| chr5_1 | exon | 4156623 | 4156770 | 4156623 | ID=Msa0712300-mRNA-1:exon:1671;Parent=Msa0712300-mRNA-1 |
| chr5_1 | CDS | 4157223 | 4157353 | 4157223 | ID=Msa0712300-mRNA-1:cds;Parent=Msa0712300-mRNA-1 |
| chr5_1 | CDS | 4157054 | 4157117 | 4157054 | ID=Msa0712300-mRNA-1:cds;Parent=Msa0712300-mRNA-1 |
| chr5_1 | CDS | 4156859 | 4156932 | 4156859 | ID=Msa0712300-mRNA-1:cds;Parent=Msa0712300-mRNA-1 |
| chr5_1 | CDS | 4156623 | 4156770 | 4156623 | ID=Msa0712300-mRNA-1:cds;Parent=Msa0712300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
