AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0712710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712710 MtrunA17_Chr5g0406071 91.860 172 13 1 1 172 1 171 1.47e-107 304
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712710 AT2G17710.1 50.360 139 43 3 36 172 38 152 1.99e-38 129

Find 63 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCCCATGTGTGTTATT+TGG 0.092661 5_1:-4531188 Msa0712710:three_prime_UTR
TATGTCAACTGGTCAATTTC+TGG 0.137128 5_1:-4531252 Msa0712710:three_prime_UTR
CAAATTGATAGTCTCTTTAT+TGG 0.141487 5_1:+4532412 None:intergenic
ATCACCAGTAATAGAATTTC+TGG 0.152523 5_1:-4531071 Msa0712710:three_prime_UTR
TTTGAGATGATTCGGAATTT+TGG 0.208841 5_1:+4532933 None:intergenic
GTTCTCCCCACGCAAGTAAA+AGG 0.227857 5_1:-4533212 Msa0712710:five_prime_UTR
AAATCCCATGTGTGTTATTT+GGG 0.285292 5_1:-4531187 Msa0712710:three_prime_UTR
TAAGTAGAGGTCCGTTTCTT+AGG 0.285599 5_1:+4531363 None:intergenic
AGCGCGAAATTGCCAAGTTT+TGG 0.285984 5_1:-4532884 Msa0712710:CDS
GTCACACTCTCTTCTGTACT+TGG 0.296232 5_1:+4532909 None:intergenic
CAATAAAGAGACTATCAATT+TGG 0.299593 5_1:-4532411 Msa0712710:CDS
CAAATATGCATACTTGCTTT+TGG 0.299850 5_1:+4531416 None:intergenic
GCTTTGTATCCTACTGCTAT+TGG 0.336710 5_1:-4531309 Msa0712710:CDS
ATGTCCAGAAATTCTATTAC+TGG 0.344567 5_1:+4531067 None:intergenic
CTAAAATAGATTTGAGATTA+CGG 0.369925 5_1:+4533079 None:intergenic
ATAATATCGAGTGCCTCTTA+TGG 0.373495 5_1:+4531274 None:intergenic
ATTGTTACATTTGTTACAAC+TGG 0.377760 5_1:-4531158 Msa0712710:three_prime_UTR
GAGACTATCAATTTGGTTGT+AGG 0.381164 5_1:-4532404 Msa0712710:CDS
TATTCTTTCTTACAAGCATA+AGG 0.399281 5_1:-4531331 Msa0712710:CDS
AGACTATCAATTTGGTTGTA+GGG 0.440855 5_1:-4532403 Msa0712710:CDS
TCATCTCCTTGCTGCTATCA+AGG 0.456611 5_1:-4532835 Msa0712710:CDS
TTCTCCCCACGCAAGTAAAA+GGG 0.456820 5_1:-4533211 Msa0712710:five_prime_UTR
ACTCGATATTATATGTCAAC+TGG 0.464577 5_1:-4531263 Msa0712710:three_prime_UTR
AGGAACAAGCAACCGTTTCA+AGG 0.465570 5_1:-4532989 Msa0712710:CDS
CCAACCAGCCTTAGCTTCCA+TGG 0.474612 5_1:-4531391 Msa0712710:CDS
TGTTGAAATGAAGCAAAGAA+AGG 0.479451 5_1:-4533009 Msa0712710:exon
TGAACTGAGCTTAAAGAATG+AGG 0.483526 5_1:-4532450 Msa0712710:CDS
AATCAATTCTTCTAATAGAA+AGG 0.484739 5_1:+4531042 None:intergenic
GACTATCAATTTGGTTGTAG+GGG 0.511564 5_1:-4532402 Msa0712710:CDS
ACTGGTGTAGTGTGGAAGAA+AGG 0.518191 5_1:-4533127 Msa0712710:five_prime_UTR
AGGAGGATCCATGGAAGCTA+AGG 0.520566 5_1:+4531383 None:intergenic
TTACCGTGAGGTTGCGCGCA+CGG 0.533446 5_1:+4532799 None:intergenic
CCGTTTCTTAGGAGGATCCA+TGG 0.535644 5_1:+4531374 None:intergenic
TGGTGTTGCTGCCCTTGAAA+CGG 0.539688 5_1:+4532977 None:intergenic
GTAGAGGTCCGTTTCTTAGG+AGG 0.541548 5_1:+4531366 None:intergenic
TGCGCGTGGGAATAAAAGAT+TGG 0.546641 5_1:-4532340 Msa0712710:intron
CCATGGATCCTCCTAAGAAA+CGG 0.550809 5_1:-4531374 Msa0712710:CDS
CTTATCCCAAATAACACACA+TGG 0.550961 5_1:+4531182 None:intergenic
TAGTGTGGAAGAAAGGAGGT+TGG 0.553309 5_1:-4533120 Msa0712710:five_prime_UTR
TGCTTGTAAGAAAGAATATG+TGG 0.555711 5_1:+4531336 None:intergenic
GAACTGAGCTTAAAGAATGA+GGG 0.561286 5_1:-4532449 Msa0712710:CDS
GCAAAAGCGAATTGTGGAAG+AGG 0.567524 5_1:-4532859 Msa0712710:CDS
TGCAAATGGAATTTCTTAGT+CGG 0.570973 5_1:+4533165 None:intergenic
CCATGGAAGCTAAGGCTGGT+TGG 0.571317 5_1:+4531391 None:intergenic
GGAACAAGCAACCGTTTCAA+GGG 0.579284 5_1:-4532988 Msa0712710:CDS
GTCTATCTACTGGTGTAGTG+TGG 0.586452 5_1:-4533135 Msa0712710:five_prime_UTR
GGATCCATGGAAGCTAAGGC+TGG 0.586903 5_1:+4531387 None:intergenic
ACTCCGTGCGCGCAACCTCA+CGG 0.592771 5_1:-4532802 Msa0712710:intron
TATAGGAGCAAGAGTATCTG+AGG 0.594659 5_1:-4532475 Msa0712710:intron
CAAAAGACACCAATAGCAGT+AGG 0.596240 5_1:+4531300 None:intergenic
TTGTGGCAATAAAGTTGATG+AGG 0.597067 5_1:-4532372 Msa0712710:CDS
GAATGAGGGTGATGAAGATG+AGG 0.611006 5_1:-4532435 Msa0712710:CDS
GTTGTAGGGGCAAATTATTG+TGG 0.611103 5_1:-4532389 Msa0712710:CDS
CTGCAGCCTTGATAGCAGCA+AGG 0.633031 5_1:+4532829 None:intergenic
AATATGTGGAACATAAGTAG+AGG 0.646153 5_1:+4531350 None:intergenic
TTGGAGGCAAAAGCGAATTG+TGG 0.647122 5_1:-4532865 Msa0712710:CDS
TGAGGACAAAGAAGTGCGCG+TGG 0.647667 5_1:-4532354 Msa0712710:CDS
AACAAATGAAACATTAACAA+GGG 0.647858 5_1:+4531215 None:intergenic
TAACAAATGAAACATTAACA+AGG 0.656419 5_1:+4531214 None:intergenic
TCTCCCCACGCAAGTAAAAG+GGG 0.658819 5_1:-4533210 Msa0712710:five_prime_UTR
GGTGTAGTGTGGAAGAAAGG+AGG 0.670095 5_1:-4533124 Msa0712710:five_prime_UTR
TTATCCCAAATAACACACAT+GGG 0.689145 5_1:+4531183 None:intergenic
GAGGACAAAGAAGTGCGCGT+GGG 0.694290 5_1:-4532353 Msa0712710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATCATTTTATTTTTTATG+CGG + chr5_1:4531545-4531564 None:intergenic 10.0%
!! TTTAAAAGTTAAAATATATG+AGG - chr5_1:4532367-4532386 Msa0712710:CDS 10.0%
!!! TTTTTTTTTTTAATGAGAAT+TGG + chr5_1:4532478-4532497 None:intergenic 10.0%
!!! AAATCATTTTATTTTTTATG+CGG + chr5_1:4531545-4531564 None:intergenic 10.0%
!! TTTAAAAGTTAAAATATATG+AGG - chr5_1:4532367-4532386 Msa0712710:CDS 10.0%
!!! TTTTTTTTTTTAATGAGAAT+TGG + chr5_1:4532478-4532497 None:intergenic 10.0%
!! GTCTATATAAATCAAATATA+AGG - chr5_1:4531484-4531503 Msa0712710:intron 15.0%
!!! AATTTATAAACAATTTTGCT+TGG + chr5_1:4531606-4531625 None:intergenic 15.0%
!!! ATTAACATATATTTTTCATG+TGG - chr5_1:4531631-4531650 Msa0712710:intron 15.0%
!!! ATTAATTGCTATTTGATTAT+AGG - chr5_1:4531716-4531735 Msa0712710:intron 15.0%
!!! ATTTTAACAAGATTTGTAAT+AGG - chr5_1:4531956-4531975 Msa0712710:intron 15.0%
!! AATAAAACATAAAAACGAAT+TGG - chr5_1:4532094-4532113 Msa0712710:intron 15.0%
!! AATTGAAATTTAGAAGATTA+AGG - chr5_1:4532449-4532468 Msa0712710:CDS 15.0%
!!! GAGACTAAAATTATTTTTTT+TGG - chr5_1:4532546-4532565 Msa0712710:intron 15.0%
!! GTCTATATAAATCAAATATA+AGG - chr5_1:4531484-4531503 Msa0712710:intron 15.0%
!!! AATTTATAAACAATTTTGCT+TGG + chr5_1:4531606-4531625 None:intergenic 15.0%
!!! ATTAACATATATTTTTCATG+TGG - chr5_1:4531631-4531650 Msa0712710:intron 15.0%
!!! ATTAATTGCTATTTGATTAT+AGG - chr5_1:4531716-4531735 Msa0712710:intron 15.0%
!!! ATTTTAACAAGATTTGTAAT+AGG - chr5_1:4531956-4531975 Msa0712710:intron 15.0%
!! AATAAAACATAAAAACGAAT+TGG - chr5_1:4532094-4532113 Msa0712710:intron 15.0%
!! AATTGAAATTTAGAAGATTA+AGG - chr5_1:4532449-4532468 Msa0712710:CDS 15.0%
!!! GAGACTAAAATTATTTTTTT+TGG - chr5_1:4532546-4532565 Msa0712710:intron 15.0%
!!! CTAAAATAGATTTGAGATTA+CGG + chr5_1:4531132-4531151 None:intergenic 20.0%
!!! TATTTATTTGTTAGTCATCA+AGG + chr5_1:4532041-4532060 None:intergenic 20.0%
!! TAACAATAACATCATAGAAA+AGG + chr5_1:4532660-4532679 None:intergenic 20.0%
!!! CATAAATGAAACCATTTTAA+AGG - chr5_1:4532702-4532721 Msa0712710:intron 20.0%
!! AACAAATGAAACATTAACAA+GGG + chr5_1:4532996-4533015 None:intergenic 20.0%
!! TAACAAATGAAACATTAACA+AGG + chr5_1:4532997-4533016 None:intergenic 20.0%
!! AATCAATTCTTCTAATAGAA+AGG + chr5_1:4533169-4533188 None:intergenic 20.0%
!!! CTAAAATAGATTTGAGATTA+CGG + chr5_1:4531132-4531151 None:intergenic 20.0%
!!! TATTTATTTGTTAGTCATCA+AGG + chr5_1:4532041-4532060 None:intergenic 20.0%
!! TAACAATAACATCATAGAAA+AGG + chr5_1:4532660-4532679 None:intergenic 20.0%
!!! CATAAATGAAACCATTTTAA+AGG - chr5_1:4532702-4532721 Msa0712710:intron 20.0%
!! AACAAATGAAACATTAACAA+GGG + chr5_1:4532996-4533015 None:intergenic 20.0%
!! TAACAAATGAAACATTAACA+AGG + chr5_1:4532997-4533016 None:intergenic 20.0%
!! AATCAATTCTTCTAATAGAA+AGG + chr5_1:4533169-4533188 None:intergenic 20.0%
!! TATAAACAATTTTGCTTGGA+TGG + chr5_1:4531602-4531621 None:intergenic 25.0%
!! CAAATTGATAGTCTCTTTAT+TGG + chr5_1:4531799-4531818 None:intergenic 25.0%
! CAATAAAGAGACTATCAATT+TGG - chr5_1:4531797-4531816 Msa0712710:intron 25.0%
!! TAAAAGATTGGTTAGTATCT+TGG - chr5_1:4531880-4531899 Msa0712710:intron 25.0%
! CATCAAGCTATTAAATTGTT+TGG - chr5_1:4531913-4531932 Msa0712710:intron 25.0%
!!! TTGATTTAGTTCTAATAGTC+CGG - chr5_1:4532153-4532172 Msa0712710:intron 25.0%
! GATTGAATCATGATGAATTA+TGG + chr5_1:4532211-4532230 None:intergenic 25.0%
!!! TGGTTGTACTCTTTTAAAAT+CGG + chr5_1:4532245-4532264 None:intergenic 25.0%
!! AACTACAAACTCCTTTAAAA+TGG + chr5_1:4532716-4532735 None:intergenic 25.0%
! TATTCTTTCTTACAAGCATA+AGG - chr5_1:4532877-4532896 Msa0712710:CDS 25.0%
!! ATTGTTACATTTGTTACAAC+TGG - chr5_1:4533050-4533069 Msa0712710:five_prime_UTR 25.0%
!! TATAAACAATTTTGCTTGGA+TGG + chr5_1:4531602-4531621 None:intergenic 25.0%
!! CAAATTGATAGTCTCTTTAT+TGG + chr5_1:4531799-4531818 None:intergenic 25.0%
! CAATAAAGAGACTATCAATT+TGG - chr5_1:4531797-4531816 Msa0712710:intron 25.0%
!! TAAAAGATTGGTTAGTATCT+TGG - chr5_1:4531880-4531899 Msa0712710:intron 25.0%
! CATCAAGCTATTAAATTGTT+TGG - chr5_1:4531913-4531932 Msa0712710:intron 25.0%
!!! TTGATTTAGTTCTAATAGTC+CGG - chr5_1:4532153-4532172 Msa0712710:intron 25.0%
! GATTGAATCATGATGAATTA+TGG + chr5_1:4532211-4532230 None:intergenic 25.0%
!!! TGGTTGTACTCTTTTAAAAT+CGG + chr5_1:4532245-4532264 None:intergenic 25.0%
!! AACTACAAACTCCTTTAAAA+TGG + chr5_1:4532716-4532735 None:intergenic 25.0%
! TATTCTTTCTTACAAGCATA+AGG - chr5_1:4532877-4532896 Msa0712710:CDS 25.0%
!! ATTGTTACATTTGTTACAAC+TGG - chr5_1:4533050-4533069 Msa0712710:five_prime_UTR 25.0%
TGCAAATGGAATTTCTTAGT+CGG + chr5_1:4531046-4531065 None:intergenic 30.0%
! GATAGACTTTTTGTTGCAAA+TGG + chr5_1:4531060-4531079 None:intergenic 30.0%
TGTTGAAATGAAGCAAAGAA+AGG - chr5_1:4531199-4531218 Msa0712710:three_prime_UTR 30.0%
!! TTTGAGATGATTCGGAATTT+TGG + chr5_1:4531278-4531297 None:intergenic 30.0%
!!! TACTTGGTTTTGAGATGATT+CGG + chr5_1:4531286-4531305 None:intergenic 30.0%
AGACTATCAATTTGGTTGTA+GGG - chr5_1:4531805-4531824 Msa0712710:intron 30.0%
GCTATTAAATTGTTTGGACA+AGG - chr5_1:4531919-4531938 Msa0712710:intron 30.0%
TAAAAACGAATTGGTTGAAC+CGG - chr5_1:4532103-4532122 Msa0712710:intron 30.0%
GAATCATGATGAATTATGGT+TGG + chr5_1:4532207-4532226 None:intergenic 30.0%
!!! CTGTCTAGTTTCATTTTTTG+TGG + chr5_1:4532393-4532412 None:intergenic 30.0%
TGCTATTATCACTGAGATTA+AGG + chr5_1:4532430-4532449 None:intergenic 30.0%
GAGTGAAAGTAAAGAAATAC+AGG - chr5_1:4532513-4532532 Msa0712710:intron 30.0%
AACATCATAGAAAAGGTTGT+GGG + chr5_1:4532653-4532672 None:intergenic 30.0%
TAACATCATAGAAAAGGTTG+TGG + chr5_1:4532654-4532673 None:intergenic 30.0%
!!! GTATTAGTTTTGTCATGTAC+AGG - chr5_1:4532765-4532784 Msa0712710:intron 30.0%
! CAAATATGCATACTTGCTTT+TGG + chr5_1:4532795-4532814 None:intergenic 30.0%
AATATGTGGAACATAAGTAG+AGG + chr5_1:4532861-4532880 None:intergenic 30.0%
TGCTTGTAAGAAAGAATATG+TGG + chr5_1:4532875-4532894 None:intergenic 30.0%
ACTCGATATTATATGTCAAC+TGG - chr5_1:4532945-4532964 Msa0712710:CDS 30.0%
! AAAATCCCATGTGTGTTATT+TGG - chr5_1:4533020-4533039 Msa0712710:exon 30.0%
!! AAATCCCATGTGTGTTATTT+GGG - chr5_1:4533021-4533040 Msa0712710:exon 30.0%
TTATCCCAAATAACACACAT+GGG + chr5_1:4533028-4533047 None:intergenic 30.0%
!!! TGACTTGCTATTCTATTTTC+TGG - chr5_1:4533107-4533126 Msa0712710:five_prime_UTR 30.0%
ATCACCAGTAATAGAATTTC+TGG - chr5_1:4533137-4533156 Msa0712710:five_prime_UTR 30.0%
ATGTCCAGAAATTCTATTAC+TGG + chr5_1:4533144-4533163 None:intergenic 30.0%
TGCAAATGGAATTTCTTAGT+CGG + chr5_1:4531046-4531065 None:intergenic 30.0%
! GATAGACTTTTTGTTGCAAA+TGG + chr5_1:4531060-4531079 None:intergenic 30.0%
TGTTGAAATGAAGCAAAGAA+AGG - chr5_1:4531199-4531218 Msa0712710:three_prime_UTR 30.0%
!! TTTGAGATGATTCGGAATTT+TGG + chr5_1:4531278-4531297 None:intergenic 30.0%
!!! TACTTGGTTTTGAGATGATT+CGG + chr5_1:4531286-4531305 None:intergenic 30.0%
AGACTATCAATTTGGTTGTA+GGG - chr5_1:4531805-4531824 Msa0712710:intron 30.0%
GCTATTAAATTGTTTGGACA+AGG - chr5_1:4531919-4531938 Msa0712710:intron 30.0%
TAAAAACGAATTGGTTGAAC+CGG - chr5_1:4532103-4532122 Msa0712710:intron 30.0%
GAATCATGATGAATTATGGT+TGG + chr5_1:4532207-4532226 None:intergenic 30.0%
!!! CTGTCTAGTTTCATTTTTTG+TGG + chr5_1:4532393-4532412 None:intergenic 30.0%
TGCTATTATCACTGAGATTA+AGG + chr5_1:4532430-4532449 None:intergenic 30.0%
GAGTGAAAGTAAAGAAATAC+AGG - chr5_1:4532513-4532532 Msa0712710:intron 30.0%
AACATCATAGAAAAGGTTGT+GGG + chr5_1:4532653-4532672 None:intergenic 30.0%
TAACATCATAGAAAAGGTTG+TGG + chr5_1:4532654-4532673 None:intergenic 30.0%
!!! GTATTAGTTTTGTCATGTAC+AGG - chr5_1:4532765-4532784 Msa0712710:intron 30.0%
! CAAATATGCATACTTGCTTT+TGG + chr5_1:4532795-4532814 None:intergenic 30.0%
AATATGTGGAACATAAGTAG+AGG + chr5_1:4532861-4532880 None:intergenic 30.0%
TGCTTGTAAGAAAGAATATG+TGG + chr5_1:4532875-4532894 None:intergenic 30.0%
ACTCGATATTATATGTCAAC+TGG - chr5_1:4532945-4532964 Msa0712710:CDS 30.0%
! AAAATCCCATGTGTGTTATT+TGG - chr5_1:4533020-4533039 Msa0712710:exon 30.0%
!! AAATCCCATGTGTGTTATTT+GGG - chr5_1:4533021-4533040 Msa0712710:exon 30.0%
TTATCCCAAATAACACACAT+GGG + chr5_1:4533028-4533047 None:intergenic 30.0%
!!! TGACTTGCTATTCTATTTTC+TGG - chr5_1:4533107-4533126 Msa0712710:five_prime_UTR 30.0%
ATCACCAGTAATAGAATTTC+TGG - chr5_1:4533137-4533156 Msa0712710:five_prime_UTR 30.0%
ATGTCCAGAAATTCTATTAC+TGG + chr5_1:4533144-4533163 None:intergenic 30.0%
GCAACAAAAAGTCTATCTAC+TGG - chr5_1:4531063-4531082 Msa0712710:three_prime_UTR 35.0%
!! ATGATTCGGAATTTTGGAAG+CGG + chr5_1:4531272-4531291 None:intergenic 35.0%
GTGTGCATGTGTATGTATTA+TGG - chr5_1:4531514-4531533 Msa0712710:intron 35.0%
AGCTACAAAGATGATCTAGA+AGG + chr5_1:4531579-4531598 None:intergenic 35.0%
TGAACTGAGCTTAAAGAATG+AGG - chr5_1:4531758-4531777 Msa0712710:intron 35.0%
GAACTGAGCTTAAAGAATGA+GGG - chr5_1:4531759-4531778 Msa0712710:intron 35.0%
GAGACTATCAATTTGGTTGT+AGG - chr5_1:4531804-4531823 Msa0712710:intron 35.0%
GACTATCAATTTGGTTGTAG+GGG - chr5_1:4531806-4531825 Msa0712710:intron 35.0%
!! TTGTGGCAATAAAGTTGATG+AGG - chr5_1:4531836-4531855 Msa0712710:intron 35.0%
GAGATCAATAGTTTGAACCA+CGG - chr5_1:4532011-4532030 Msa0712710:intron 35.0%
CTGACAGATTGATTGATTAC+TGG - chr5_1:4532280-4532299 Msa0712710:intron 35.0%
! ATTGATTACTGGTTAAACCG+AGG - chr5_1:4532291-4532310 Msa0712710:intron 35.0%
ATTATCACTGAGATTAAGGC+AGG + chr5_1:4532426-4532445 None:intergenic 35.0%
ACATCATAGAAAAGGTTGTG+GGG + chr5_1:4532652-4532671 None:intergenic 35.0%
!!! TTAGTTTTGTCATGTACAGG+TGG - chr5_1:4532768-4532787 Msa0712710:intron 35.0%
ATAATATCGAGTGCCTCTTA+TGG + chr5_1:4532937-4532956 None:intergenic 35.0%
TATGTCAACTGGTCAATTTC+TGG - chr5_1:4532956-4532975 Msa0712710:CDS 35.0%
CTTATCCCAAATAACACACA+TGG + chr5_1:4533029-4533048 None:intergenic 35.0%
GCAACAAAAAGTCTATCTAC+TGG - chr5_1:4531063-4531082 Msa0712710:three_prime_UTR 35.0%
!! ATGATTCGGAATTTTGGAAG+CGG + chr5_1:4531272-4531291 None:intergenic 35.0%
GTGTGCATGTGTATGTATTA+TGG - chr5_1:4531514-4531533 Msa0712710:intron 35.0%
AGCTACAAAGATGATCTAGA+AGG + chr5_1:4531579-4531598 None:intergenic 35.0%
TGAACTGAGCTTAAAGAATG+AGG - chr5_1:4531758-4531777 Msa0712710:intron 35.0%
GAACTGAGCTTAAAGAATGA+GGG - chr5_1:4531759-4531778 Msa0712710:intron 35.0%
GAGACTATCAATTTGGTTGT+AGG - chr5_1:4531804-4531823 Msa0712710:intron 35.0%
GACTATCAATTTGGTTGTAG+GGG - chr5_1:4531806-4531825 Msa0712710:intron 35.0%
!! TTGTGGCAATAAAGTTGATG+AGG - chr5_1:4531836-4531855 Msa0712710:intron 35.0%
GAGATCAATAGTTTGAACCA+CGG - chr5_1:4532011-4532030 Msa0712710:intron 35.0%
CTGACAGATTGATTGATTAC+TGG - chr5_1:4532280-4532299 Msa0712710:intron 35.0%
! ATTGATTACTGGTTAAACCG+AGG - chr5_1:4532291-4532310 Msa0712710:intron 35.0%
ATTATCACTGAGATTAAGGC+AGG + chr5_1:4532426-4532445 None:intergenic 35.0%
ACATCATAGAAAAGGTTGTG+GGG + chr5_1:4532652-4532671 None:intergenic 35.0%
!!! TTAGTTTTGTCATGTACAGG+TGG - chr5_1:4532768-4532787 Msa0712710:intron 35.0%
ATAATATCGAGTGCCTCTTA+TGG + chr5_1:4532937-4532956 None:intergenic 35.0%
TATGTCAACTGGTCAATTTC+TGG - chr5_1:4532956-4532975 Msa0712710:CDS 35.0%
CTTATCCCAAATAACACACA+TGG + chr5_1:4533029-4533048 None:intergenic 35.0%
!! TGATTCGGAATTTTGGAAGC+GGG + chr5_1:4531271-4531290 None:intergenic 40.0%
! ACTATATGTCACTTACCGTG+AGG + chr5_1:4531424-4531443 None:intergenic 40.0%
ATTATAGATAGCTGCTGCAG+AGG + chr5_1:4531453-4531472 None:intergenic 40.0%
!! TATAGGAGCAAGAGTATCTG+AGG - chr5_1:4531733-4531752 Msa0712710:intron 40.0%
GTTGTAGGGGCAAATTATTG+TGG - chr5_1:4531819-4531838 Msa0712710:intron 40.0%
! AATCTGTCAGTTGGTTCAAC+CGG + chr5_1:4532175-4532194 None:intergenic 40.0%
TTCATTGCCAATCTGTCAGT+TGG + chr5_1:4532184-4532203 None:intergenic 40.0%
GTCAGTTGTTCAACTGAAGT+TGG + chr5_1:4532265-4532284 None:intergenic 40.0%
TTCATCATCGAAAGAGACCT+CGG + chr5_1:4532311-4532330 None:intergenic 40.0%
TAAGTAGAGGTCCGTTTCTT+AGG + chr5_1:4532848-4532867 None:intergenic 40.0%
GCTTTGTATCCTACTGCTAT+TGG - chr5_1:4532899-4532918 Msa0712710:CDS 40.0%
CAAAAGACACCAATAGCAGT+AGG + chr5_1:4532911-4532930 None:intergenic 40.0%
!!! GTGTCTTTTGAAGCCATAAG+AGG - chr5_1:4532921-4532940 Msa0712710:CDS 40.0%
!! TGATTCGGAATTTTGGAAGC+GGG + chr5_1:4531271-4531290 None:intergenic 40.0%
! ACTATATGTCACTTACCGTG+AGG + chr5_1:4531424-4531443 None:intergenic 40.0%
ATTATAGATAGCTGCTGCAG+AGG + chr5_1:4531453-4531472 None:intergenic 40.0%
!! TATAGGAGCAAGAGTATCTG+AGG - chr5_1:4531733-4531752 Msa0712710:intron 40.0%
GTTGTAGGGGCAAATTATTG+TGG - chr5_1:4531819-4531838 Msa0712710:intron 40.0%
! AATCTGTCAGTTGGTTCAAC+CGG + chr5_1:4532175-4532194 None:intergenic 40.0%
TTCATTGCCAATCTGTCAGT+TGG + chr5_1:4532184-4532203 None:intergenic 40.0%
GTCAGTTGTTCAACTGAAGT+TGG + chr5_1:4532265-4532284 None:intergenic 40.0%
TTCATCATCGAAAGAGACCT+CGG + chr5_1:4532311-4532330 None:intergenic 40.0%
TAAGTAGAGGTCCGTTTCTT+AGG + chr5_1:4532848-4532867 None:intergenic 40.0%
GCTTTGTATCCTACTGCTAT+TGG - chr5_1:4532899-4532918 Msa0712710:CDS 40.0%
CAAAAGACACCAATAGCAGT+AGG + chr5_1:4532911-4532930 None:intergenic 40.0%
!!! GTGTCTTTTGAAGCCATAAG+AGG - chr5_1:4532921-4532940 Msa0712710:CDS 40.0%
TTCTCCCCACGCAAGTAAAA+GGG - chr5_1:4530997-4531016 Msa0712710:three_prime_UTR 45.0%
! ATAGCCCCTTTTACTTGCGT+GGG + chr5_1:4531005-4531024 None:intergenic 45.0%
! TATAGCCCCTTTTACTTGCG+TGG + chr5_1:4531006-4531025 None:intergenic 45.0%
GTCTATCTACTGGTGTAGTG+TGG - chr5_1:4531073-4531092 Msa0712710:three_prime_UTR 45.0%
ACTGGTGTAGTGTGGAAGAA+AGG - chr5_1:4531081-4531100 Msa0712710:three_prime_UTR 45.0%
TAGTGTGGAAGAAAGGAGGT+TGG - chr5_1:4531088-4531107 Msa0712710:three_prime_UTR 45.0%
AGGAACAAGCAACCGTTTCA+AGG - chr5_1:4531219-4531238 Msa0712710:three_prime_UTR 45.0%
GGAACAAGCAACCGTTTCAA+GGG - chr5_1:4531220-4531239 Msa0712710:three_prime_UTR 45.0%
!! ATTCGGAATTTTGGAAGCGG+GGG + chr5_1:4531269-4531288 None:intergenic 45.0%
!! GATTCGGAATTTTGGAAGCG+GGG + chr5_1:4531270-4531289 None:intergenic 45.0%
GTCACACTCTCTTCTGTACT+TGG + chr5_1:4531302-4531321 None:intergenic 45.0%
! AGCGCGAAATTGCCAAGTTT+TGG - chr5_1:4531324-4531343 Msa0712710:CDS 45.0%
!! GCGAAATTGCCAAGTTTTGG+AGG - chr5_1:4531327-4531346 Msa0712710:CDS 45.0%
!! TCGCTTTTGCCTCCAAAACT+TGG + chr5_1:4531339-4531358 None:intergenic 45.0%
TTGGAGGCAAAAGCGAATTG+TGG - chr5_1:4531343-4531362 Msa0712710:CDS 45.0%
GCAAAAGCGAATTGTGGAAG+AGG - chr5_1:4531349-4531368 Msa0712710:CDS 45.0%
TCATCTCCTTGCTGCTATCA+AGG - chr5_1:4531373-4531392 Msa0712710:CDS 45.0%
GAATGAGGGTGATGAAGATG+AGG - chr5_1:4531773-4531792 Msa0712710:intron 45.0%
TGCGCGTGGGAATAAAAGAT+TGG - chr5_1:4531868-4531887 Msa0712710:intron 45.0%
TTAGTCATCAAGGTGAACCG+TGG + chr5_1:4532031-4532050 None:intergenic 45.0%
GGTTGAACCAACTGACAGAT+TGG - chr5_1:4532174-4532193 Msa0712710:intron 45.0%
CCATGGATCCTCCTAAGAAA+CGG - chr5_1:4532834-4532853 Msa0712710:CDS 45.0%
TTCTCCCCACGCAAGTAAAA+GGG - chr5_1:4530997-4531016 Msa0712710:three_prime_UTR 45.0%
! ATAGCCCCTTTTACTTGCGT+GGG + chr5_1:4531005-4531024 None:intergenic 45.0%
! TATAGCCCCTTTTACTTGCG+TGG + chr5_1:4531006-4531025 None:intergenic 45.0%
GTCTATCTACTGGTGTAGTG+TGG - chr5_1:4531073-4531092 Msa0712710:three_prime_UTR 45.0%
ACTGGTGTAGTGTGGAAGAA+AGG - chr5_1:4531081-4531100 Msa0712710:three_prime_UTR 45.0%
TAGTGTGGAAGAAAGGAGGT+TGG - chr5_1:4531088-4531107 Msa0712710:three_prime_UTR 45.0%
AGGAACAAGCAACCGTTTCA+AGG - chr5_1:4531219-4531238 Msa0712710:three_prime_UTR 45.0%
GGAACAAGCAACCGTTTCAA+GGG - chr5_1:4531220-4531239 Msa0712710:three_prime_UTR 45.0%
!! ATTCGGAATTTTGGAAGCGG+GGG + chr5_1:4531269-4531288 None:intergenic 45.0%
!! GATTCGGAATTTTGGAAGCG+GGG + chr5_1:4531270-4531289 None:intergenic 45.0%
GTCACACTCTCTTCTGTACT+TGG + chr5_1:4531302-4531321 None:intergenic 45.0%
! AGCGCGAAATTGCCAAGTTT+TGG - chr5_1:4531324-4531343 Msa0712710:CDS 45.0%
!! GCGAAATTGCCAAGTTTTGG+AGG - chr5_1:4531327-4531346 Msa0712710:CDS 45.0%
!! TCGCTTTTGCCTCCAAAACT+TGG + chr5_1:4531339-4531358 None:intergenic 45.0%
TTGGAGGCAAAAGCGAATTG+TGG - chr5_1:4531343-4531362 Msa0712710:CDS 45.0%
GCAAAAGCGAATTGTGGAAG+AGG - chr5_1:4531349-4531368 Msa0712710:CDS 45.0%
TCATCTCCTTGCTGCTATCA+AGG - chr5_1:4531373-4531392 Msa0712710:CDS 45.0%
GAATGAGGGTGATGAAGATG+AGG - chr5_1:4531773-4531792 Msa0712710:intron 45.0%
TGCGCGTGGGAATAAAAGAT+TGG - chr5_1:4531868-4531887 Msa0712710:intron 45.0%
TTAGTCATCAAGGTGAACCG+TGG + chr5_1:4532031-4532050 None:intergenic 45.0%
GGTTGAACCAACTGACAGAT+TGG - chr5_1:4532174-4532193 Msa0712710:intron 45.0%
CCATGGATCCTCCTAAGAAA+CGG - chr5_1:4532834-4532853 Msa0712710:CDS 45.0%
!! AAAAAAAAAAAAAAAACTAA+TGG - chr5_1:4532487-4532506 Msa0712710:intron 5.0%
!! AAAAAAAAAAAAAAAACTAA+TGG - chr5_1:4532487-4532506 Msa0712710:intron 5.0%
GTTCTCCCCACGCAAGTAAA+AGG - chr5_1:4530996-4531015 Msa0712710:three_prime_UTR 50.0%
TCTCCCCACGCAAGTAAAAG+GGG - chr5_1:4530998-4531017 Msa0712710:three_prime_UTR 50.0%
! TAGCCCCTTTTACTTGCGTG+GGG + chr5_1:4531004-4531023 None:intergenic 50.0%
GGTGTAGTGTGGAAGAAAGG+AGG - chr5_1:4531084-4531103 Msa0712710:three_prime_UTR 50.0%
!! TGGTGTTGCTGCCCTTGAAA+CGG + chr5_1:4531234-4531253 None:intergenic 50.0%
GAATGAATCGAGCTGAGCCA+TGG + chr5_1:4532129-4532148 None:intergenic 50.0%
AGGAGGATCCATGGAAGCTA+AGG + chr5_1:4532828-4532847 None:intergenic 50.0%
CCGTTTCTTAGGAGGATCCA+TGG + chr5_1:4532837-4532856 None:intergenic 50.0%
GTAGAGGTCCGTTTCTTAGG+AGG + chr5_1:4532845-4532864 None:intergenic 50.0%
TACTTGCGTGGGGAGAACAT+GGG + chr5_1:4530994-4531013 None:intergenic 50.0%
TTACTTGCGTGGGGAGAACA+TGG + chr5_1:4530995-4531014 None:intergenic 50.0%
GTTCTCCCCACGCAAGTAAA+AGG - chr5_1:4530996-4531015 Msa0712710:three_prime_UTR 50.0%
TCTCCCCACGCAAGTAAAAG+GGG - chr5_1:4530998-4531017 Msa0712710:three_prime_UTR 50.0%
! TAGCCCCTTTTACTTGCGTG+GGG + chr5_1:4531004-4531023 None:intergenic 50.0%
GGTGTAGTGTGGAAGAAAGG+AGG - chr5_1:4531084-4531103 Msa0712710:three_prime_UTR 50.0%
!! TGGTGTTGCTGCCCTTGAAA+CGG + chr5_1:4531234-4531253 None:intergenic 50.0%
GAATGAATCGAGCTGAGCCA+TGG + chr5_1:4532129-4532148 None:intergenic 50.0%
AGGAGGATCCATGGAAGCTA+AGG + chr5_1:4532828-4532847 None:intergenic 50.0%
CCGTTTCTTAGGAGGATCCA+TGG + chr5_1:4532837-4532856 None:intergenic 50.0%
GTAGAGGTCCGTTTCTTAGG+AGG + chr5_1:4532845-4532864 None:intergenic 50.0%
!! AATTTTGGAAGCGGGGGAGG+AGG + chr5_1:4531263-4531282 None:intergenic 55.0%
! CTGCAGCCTTGATAGCAGCA+AGG + chr5_1:4531382-4531401 None:intergenic 55.0%
! TGAGGACAAAGAAGTGCGCG+TGG - chr5_1:4531854-4531873 Msa0712710:intron 55.0%
! GAGGACAAAGAAGTGCGCGT+GGG - chr5_1:4531855-4531874 Msa0712710:intron 55.0%
CGAATTGGTTGAACCGGCCA+TGG - chr5_1:4532109-4532128 Msa0712710:intron 55.0%
CCATGGAAGCTAAGGCTGGT+TGG + chr5_1:4532820-4532839 None:intergenic 55.0%
CCAACCAGCCTTAGCTTCCA+TGG - chr5_1:4532817-4532836 Msa0712710:CDS 55.0%
GGATCCATGGAAGCTAAGGC+TGG + chr5_1:4532824-4532843 None:intergenic 55.0%
!! AATTTTGGAAGCGGGGGAGG+AGG + chr5_1:4531263-4531282 None:intergenic 55.0%
! CTGCAGCCTTGATAGCAGCA+AGG + chr5_1:4531382-4531401 None:intergenic 55.0%
! TGAGGACAAAGAAGTGCGCG+TGG - chr5_1:4531854-4531873 Msa0712710:intron 55.0%
! GAGGACAAAGAAGTGCGCGT+GGG - chr5_1:4531855-4531874 Msa0712710:intron 55.0%
CGAATTGGTTGAACCGGCCA+TGG - chr5_1:4532109-4532128 Msa0712710:intron 55.0%
CCATGGAAGCTAAGGCTGGT+TGG + chr5_1:4532820-4532839 None:intergenic 55.0%
CCAACCAGCCTTAGCTTCCA+TGG - chr5_1:4532817-4532836 Msa0712710:CDS 55.0%
GGATCCATGGAAGCTAAGGC+TGG + chr5_1:4532824-4532843 None:intergenic 55.0%
!! CGGAATTTTGGAAGCGGGGG+AGG + chr5_1:4531266-4531285 None:intergenic 60.0%
TTACCGTGAGGTTGCGCGCA+CGG + chr5_1:4531412-4531431 None:intergenic 60.0%
GAATCGAGCTGAGCCATGGC+CGG + chr5_1:4532125-4532144 None:intergenic 60.0%
!! CGGAATTTTGGAAGCGGGGG+AGG + chr5_1:4531266-4531285 None:intergenic 60.0%
TTACCGTGAGGTTGCGCGCA+CGG + chr5_1:4531412-4531431 None:intergenic 60.0%
GAATCGAGCTGAGCCATGGC+CGG + chr5_1:4532125-4532144 None:intergenic 60.0%
TTTGGAAGCGGGGGAGGAGG+AGG + chr5_1:4531260-4531279 None:intergenic 65.0%
ACTCCGTGCGCGCAACCTCA+CGG - chr5_1:4531406-4531425 Msa0712710:CDS 65.0%
TTTGGAAGCGGGGGAGGAGG+AGG + chr5_1:4531260-4531279 None:intergenic 65.0%
ACTCCGTGCGCGCAACCTCA+CGG - chr5_1:4531406-4531425 Msa0712710:CDS 65.0%
AGCGGGGGAGGAGGAGGTGG+TGG + chr5_1:4531254-4531273 None:intergenic 75.0%
GGAAGCGGGGGAGGAGGAGG+TGG + chr5_1:4531257-4531276 None:intergenic 75.0%
AGCGGGGGAGGAGGAGGTGG+TGG + chr5_1:4531254-4531273 None:intergenic 75.0%
GGAAGCGGGGGAGGAGGAGG+TGG + chr5_1:4531257-4531276 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 4530994 4533236 4530994 ID=Msa0712710;Name=Msa0712710
chr5_1 mRNA 4530994 4533236 4530994 ID=Msa0712710-mRNA-1;Parent=Msa0712710;Name=Msa0712710-mRNA-1;_AED=0.12;_eAED=0.12;_QI=212|1|1|1|1|1|3|305|172
chr5_1 exon 4530994 4531443 4530994 ID=Msa0712710-mRNA-1:exon:1891;Parent=Msa0712710-mRNA-1
chr5_1 exon 4532341 4532492 4532341 ID=Msa0712710-mRNA-1:exon:1890;Parent=Msa0712710-mRNA-1
chr5_1 exon 4532803 4533236 4532803 ID=Msa0712710-mRNA-1:exon:1889;Parent=Msa0712710-mRNA-1
chr5_1 five_prime_UTR 4533025 4533236 4533025 ID=Msa0712710-mRNA-1:five_prime_utr;Parent=Msa0712710-mRNA-1
chr5_1 CDS 4532803 4533024 4532803 ID=Msa0712710-mRNA-1:cds;Parent=Msa0712710-mRNA-1
chr5_1 CDS 4532341 4532492 4532341 ID=Msa0712710-mRNA-1:cds;Parent=Msa0712710-mRNA-1
chr5_1 CDS 4531299 4531443 4531299 ID=Msa0712710-mRNA-1:cds;Parent=Msa0712710-mRNA-1
chr5_1 three_prime_UTR 4530994 4531298 4530994 ID=Msa0712710-mRNA-1:three_prime_utr;Parent=Msa0712710-mRNA-1
Gene Sequence

>Msa0712710

ATGAAGCAAAGAAAGGAACAAGCAACCGTTTCAAGGGCAGCAACACCACCACCTCCTCCTCCCCCGCTTCCAAAATTCCGAATCATCTCAAAACCAAGTACAGAAGAGAGTGTGACAAAGCGCGAAATTGCCAAGTTTTGGAGGCAAAAGCGAATTGTGGAAGAGGATCATCTCCTTGCTGCTATCAAGGCTGCAGCAAGACTCCGTGCGCGCAACCTCACGGAGCAAGAGTATCTGAGGTTTGAACTGAGCTTAAAGAATGAGGGTGATGAAGATGAGGCCAATAAAGAGACTATCAATTTGGTTGTAGGGGCAAATTATTGTGGCAATAAAGTTGATGAGGACAAAGAAGTGCGCGTGGGAATAAAAGATTGGTGGACCAAAAGCAAGTATGCATATTTGAACCAACCAGCCTTAGCTTCCATGGATCCTCCTAAGAAACGGACCTCTACTTATGTTCCACATATTCTTTCTTACAAGCATAAGGCTTTGTATCCTACTGCTATTGGTGTCTTTTGA
Protein sequence

>Msa0712710

MKQRKEQATVSRAATPPPPPPPLPKFRIISKPSTEESVTKREIAKFWRQKRIVEEDHLLAAIKAAARLRARNLTEQEYLRFELSLKNEGDEDEANKETINLVVGANYCGNKVDEDKEVRVGIKDWWTKSKYAYLNQPALASMDPPKKRTSTYVPHILSYKHKALYPTAIGVF*