AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0713860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0713860 MtrunA17_Chr5g0401131 96.774 496 9 2 1 494 1 491 0.0 993
Msa0713860 MtrunA17_Chr2g0329591 54.545 396 155 7 113 494 68 452 1.57e-144 422
Msa0713860 MtrunA17_Chr4g0054071 61.224 245 73 7 1 227 1 241 6.48e-70 223
Msa0713860 MtrunA17_Chr5g0447691 30.000 390 252 7 111 493 169 544 3.89e-56 196
Msa0713860 MtrunA17_Chr2g0319091 32.105 380 239 7 119 492 212 578 4.60e-56 196
Msa0713860 MtrunA17_Chr1g0159461 31.316 380 244 8 119 493 225 592 9.50e-56 196
Msa0713860 MtrunA17_Chr5g0445331 30.809 383 246 7 119 493 161 532 3.03e-54 190
Msa0713860 MtrunA17_Chr3g0125811 31.316 380 244 8 119 493 203 570 4.83e-52 185
Msa0713860 MtrunA17_Chr3g0101141 27.132 516 318 15 9 493 54 542 3.54e-51 182
Msa0713860 MtrunA17_Chr4g0053901 28.446 457 280 10 80 494 18 469 4.14e-50 178
Msa0713860 MtrunA17_Chr7g0224271 31.658 398 227 13 118 493 299 673 4.50e-48 176
Msa0713860 MtrunA17_Chr5g0401421 27.991 443 259 12 99 494 18 447 6.84e-47 169
Msa0713860 MtrunA17_Chr2g0290241 29.444 360 233 8 141 493 80 425 9.34e-47 167
Msa0713860 MtrunA17_Chr3g0110971 28.448 464 278 10 61 493 147 587 6.35e-46 169
Msa0713860 MtrunA17_Chr4g0076091 29.167 384 246 10 119 492 175 542 9.15e-45 165
Msa0713860 MtrunA17_Chr8g0344101 31.436 404 234 13 113 493 217 600 8.79e-44 163
Msa0713860 MtrunA17_Chr8g0376381 30.789 380 230 10 120 493 170 522 2.30e-43 160
Msa0713860 MtrunA17_Chr8g0357601 29.474 380 252 9 120 493 183 552 6.43e-42 157
Msa0713860 MtrunA17_Chr4g0057291 27.979 386 256 11 118 494 291 663 7.19e-42 158
Msa0713860 MtrunA17_Chr7g0245601 28.571 378 240 13 122 494 441 793 2.62e-41 157
Msa0713860 MtrunA17_Chr2g0306641 26.994 489 308 13 20 493 33 487 3.66e-41 154
Msa0713860 MtrunA17_Chr4g0031741 29.058 382 251 9 119 494 284 651 2.15e-40 154
Msa0713860 MtrunA17_Chr4g0031711 26.842 380 270 4 118 494 360 734 2.98e-38 148
Msa0713860 MtrunA17_Chr4g0039661 29.870 385 236 11 120 493 194 555 5.85e-38 146
Msa0713860 MtrunA17_Chr4g0038761 28.609 381 240 15 122 494 87 443 6.81e-38 144
Msa0713860 MtrunA17_Chr2g0328341 26.053 380 265 9 119 494 272 639 1.89e-37 145
Msa0713860 MtrunA17_Chr5g0396491 25.755 497 281 13 63 493 4 478 2.22e-37 143
Msa0713860 MtrunA17_Chr2g0328391 28.534 382 257 7 119 494 299 670 6.47e-37 144
Msa0713860 MtrunA17_Chr4g0031701 28.205 351 240 7 118 463 253 596 1.76e-36 142
Msa0713860 MtrunA17_Chr7g0238891 25.330 379 271 5 119 494 372 741 4.60e-35 139
Msa0713860 MtrunA17_Chr4g0031721 26.913 379 265 5 119 494 316 685 4.91e-35 139
Msa0713860 MtrunA17_Chr4g0031731 28.205 351 240 7 118 463 253 596 6.50e-35 138
Msa0713860 MtrunA17_Chr2g0328381 26.053 380 265 9 119 494 275 642 7.84e-35 138
Msa0713860 MtrunA17_Chr4g0057891 25.964 389 257 10 119 493 149 520 2.49e-34 135
Msa0713860 MtrunA17_Chr2g0328411 25.729 377 265 6 119 492 289 653 7.23e-34 135
Msa0713860 MtrunA17_Chr2g0328431 25.916 382 262 7 119 494 289 655 5.52e-33 132
Msa0713860 MtrunA17_Chr3g0085681 23.765 425 259 9 120 493 26 436 7.42e-32 127
Msa0713860 MtrunA17_Chr2g0328401 27.221 349 235 7 119 462 290 624 3.61e-31 127
Msa0713860 MtrunA17_Chr1g0183641 27.034 381 244 14 119 492 123 476 8.18e-30 122
Msa0713860 MtrunA17_Chr3g0114841 27.654 405 239 15 118 492 119 499 1.06e-27 115
Msa0713860 MtrunA17_Chr2g0328361 23.810 378 251 8 119 494 274 616 3.44e-27 115
Msa0713860 MtrunA17_Chr1g0200401 27.094 406 241 14 118 493 79 459 1.58e-26 112
Msa0713860 MtrunA17_Chr4g0038851 29.960 247 163 4 99 344 28 265 1.07e-25 109
Msa0713860 MtrunA17_Chr8g0383981 26.804 388 245 17 118 494 138 497 9.88e-25 107
Msa0713860 MtrunA17_Chr2g0294471 25.575 391 255 14 120 493 205 576 4.17e-24 105
Msa0713860 MtrunA17_Chr5g0424131 27.778 396 248 14 118 492 120 498 4.40e-24 105
Msa0713860 MtrunA17_Chr7g0236851 24.816 407 279 9 97 492 266 656 2.26e-23 103
Msa0713860 MtrunA17_Chr2g0294461 23.896 385 272 8 120 493 211 585 4.85e-23 102
Msa0713860 MtrunA17_Chr2g0323111 32.418 182 117 3 314 494 131 307 5.23e-23 99.8
Msa0713860 MtrunA17_Chr3g0085361 23.478 345 202 8 199 493 18 350 9.29e-23 99.4
Msa0713860 MtrunA17_Chr6g0468561 26.166 386 254 11 120 493 296 662 3.19e-22 100
Msa0713860 MtrunA17_Chr1g0194621 24.167 360 254 8 141 493 171 518 4.58e-20 93.2
Msa0713860 MtrunA17_Chr2g0328371 26.104 249 174 6 199 445 8 248 1.87e-19 87.8
Msa0713860 MtrunA17_Chr7g0270031 23.670 376 274 6 120 493 190 554 2.91e-19 90.9
Msa0713860 MtrunA17_Chr4g0012071 21.429 378 270 9 120 493 197 551 2.39e-16 81.6
Msa0713860 MtrunA17_Chr7g0230771 21.628 393 252 9 120 493 376 731 1.02e-14 77.0
Msa0713860 MtrunA17_Chr7g0230731 21.120 393 254 9 120 493 373 728 6.64e-14 74.3
Msa0713860 MtrunA17_Chr8g0373561 23.291 395 265 11 106 493 178 541 1.59e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0713860 AT4G37650.1 58.965 541 164 11 1 494 1 530 0.0 580
Msa0713860 AT2G04890.1 31.649 376 241 7 119 493 53 413 7.21e-60 202
Msa0713860 AT1G21450.1 32.987 385 232 9 119 493 225 593 2.02e-52 187
Msa0713860 AT5G48150.1 28.647 377 257 6 120 493 123 490 8.21e-52 183
Msa0713860 AT5G48150.4 28.647 377 257 6 120 493 123 490 8.21e-52 183
Msa0713860 AT5G48150.2 28.647 377 257 6 120 493 123 490 8.21e-52 183
Msa0713860 AT5G48150.3 28.647 377 257 6 120 493 123 490 8.21e-52 183
Msa0713860 AT1G50600.3 30.390 385 243 9 118 493 158 526 6.08e-51 182
Msa0713860 AT1G50600.1 30.469 384 242 9 119 493 230 597 1.46e-50 182
Msa0713860 AT1G50600.2 30.469 384 242 9 119 493 234 601 1.73e-50 182
Msa0713860 AT3G49950.1 32.308 390 240 10 119 494 30 409 6.77e-50 176
Msa0713860 AT3G03450.1 30.570 386 248 7 112 493 176 545 1.19e-48 176
Msa0713860 AT1G66350.1 32.468 385 231 11 112 493 148 506 3.88e-48 174
Msa0713860 AT5G17490.1 31.783 387 242 8 109 493 150 516 9.82e-48 173
Msa0713860 AT2G01570.1 29.443 377 243 10 120 493 225 581 2.30e-42 159
Msa0713860 AT4G17230.1 26.804 388 253 9 119 494 158 526 2.36e-41 155
Msa0713860 AT4G17230.2 26.804 388 253 9 119 494 158 526 2.36e-41 155
Msa0713860 AT1G14920.1 29.708 377 242 9 120 493 173 529 3.47e-41 155
Msa0713860 AT1G50420.1 25.532 470 280 7 84 493 20 479 6.20e-41 153
Msa0713860 AT3G13840.1 30.982 397 230 14 113 493 142 510 7.32e-40 151
Msa0713860 AT3G54220.1 29.058 382 236 15 122 494 296 651 2.02e-38 149
Msa0713860 AT1G63100.2 29.412 391 247 11 118 492 276 653 1.10e-37 147
Msa0713860 AT1G63100.1 29.412 391 247 11 118 492 251 628 1.21e-37 146
Msa0713860 AT5G41920.1 29.243 383 240 14 118 493 42 400 3.15e-35 136
Msa0713860 AT1G55580.1 28.398 412 248 11 119 493 44 445 1.60e-34 135
Msa0713860 AT2G29065.1 26.494 385 267 9 119 494 252 629 3.04e-34 136
Msa0713860 AT3G46600.2 27.559 381 261 8 118 494 3 372 3.60e-34 132
Msa0713860 AT3G46600.1 27.559 381 261 8 118 494 211 580 1.35e-33 134
Msa0713860 AT2G37650.1 26.316 380 266 6 118 494 346 714 1.43e-33 135
Msa0713860 AT2G37650.2 26.316 380 266 6 118 494 334 702 1.53e-33 135
Msa0713860 AT5G52510.1 27.551 392 246 9 120 493 269 640 1.72e-33 134
Msa0713860 AT5G66770.1 28.191 376 243 10 127 493 227 584 1.90e-33 134
Msa0713860 AT3G46600.3 27.559 381 261 8 118 494 179 548 2.01e-33 133
Msa0713860 AT1G07530.1 25.526 380 270 6 119 494 396 766 8.04e-33 133
Msa0713860 AT5G59450.1 26.247 381 268 6 119 494 227 599 4.01e-30 124
Msa0713860 AT2G29060.2 25.131 382 271 8 119 494 284 656 6.69e-30 124
Msa0713860 AT2G29060.1 25.131 382 271 8 119 494 321 693 6.82e-30 124
Msa0713860 AT1G07520.1 25.131 382 275 7 119 494 318 694 7.29e-30 124
Msa0713860 AT1G07520.3 25.131 382 275 7 119 494 319 695 8.55e-30 124
Msa0713860 AT1G07520.2 25.131 382 275 7 119 494 154 530 1.42e-29 122
Msa0713860 AT1G07520.4 25.131 382 275 7 119 494 154 530 1.42e-29 122
Msa0713860 AT3G50650.1 28.934 394 238 12 117 493 174 542 1.59e-29 122
Msa0713860 AT4G08250.1 27.047 403 261 16 103 492 94 476 7.49e-24 104
Msa0713860 AT4G00150.1 23.664 393 233 14 121 493 209 554 3.09e-13 72.4
Msa0713860 AT3G60630.1 24.919 309 199 12 199 493 333 622 5.42e-12 68.6

Find 118 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGGCTGATTTAAATTT+TGG 0.142361 5_1:-6322845 Msa0713860:CDS
TGAATAACGTTGAATTTAAA+TGG 0.226774 5_1:+6323055 None:intergenic
CTTCTTGTGGATTGTAATAT+TGG 0.229803 5_1:+6323782 None:intergenic
GATTTCACCTTTGAATTAAC+TGG 0.256797 5_1:-6323607 Msa0713860:CDS
AAGAGTGTTTGAGATGGTTT+AGG 0.270052 5_1:-6322790 Msa0713860:CDS
GGCTTTGTTGAGGAGGTATA+AGG 0.272310 5_1:-6322552 Msa0713860:CDS
TTCGGTCATTCGAGAGAATA+AGG 0.284815 5_1:+6323435 None:intergenic
ACCTTCGAAGGCTTCTAAAA+TGG 0.301815 5_1:+6323276 None:intergenic
GGGGTGTGAGTGATGGTGTG+GGG 0.332972 5_1:+6323644 None:intergenic
TGGGAGAGGGATAGTTGATT+TGG 0.342461 5_1:-6322696 Msa0713860:CDS
GGGTGTGAGTGATGGTGTGG+GGG 0.342781 5_1:+6323645 None:intergenic
AAATCAGTTGTTGGTGGAGG+GGG 0.353733 5_1:+6323625 None:intergenic
AGGAAGTTTGTGATGATGTT+AGG 0.369294 5_1:-6322574 Msa0713860:CDS
GAAGGTTCTGGTACCGGCTT+CGG 0.378697 5_1:+6323417 None:intergenic
AGAGTGTTTGAGATGGTTTA+GGG 0.380120 5_1:-6322789 Msa0713860:CDS
GAGGTTTGAGGTTGACTATA+AGG 0.384294 5_1:+6323688 None:intergenic
TAGTCCATGGACTAACTTCT+TGG 0.385254 5_1:+6323326 None:intergenic
CGTTGATCGCGTTGCGGCCG+AGG 0.391480 5_1:-6322886 Msa0713860:CDS
ACTTGATGTTGTTGTTCTGC+TGG 0.396312 5_1:+6323834 None:intergenic
CATTCTTCTTCAACTTCTTG+TGG 0.400632 5_1:+6323769 None:intergenic
CTTCCACGTCAAGAATATTC+CGG 0.409628 5_1:+6322499 None:intergenic
GTTAGTCCATGGACTACTTT+CGG 0.413327 5_1:-6323319 Msa0713860:CDS
AGCTAGGGTTCTGAAGGTTC+TGG 0.415747 5_1:+6323405 None:intergenic
GTTTGTAGTAATGGTGTTTG+AGG 0.420379 5_1:+6323669 None:intergenic
GTTGTTGGTGTTGGTGTGGT+TGG 0.425166 5_1:+6323893 None:intergenic
ACTATAAGGATAGTAGTGTT+TGG 0.430455 5_1:+6323702 None:intergenic
GAAGGAAATTGGTGCTAGAA+TGG 0.434419 5_1:-6323104 Msa0713860:CDS
AACGAACTAAGCTCCCCTTA+TGG 0.445036 5_1:-6323496 Msa0713860:CDS
GTAATGGTGTTTGAGGTTTG+AGG 0.447709 5_1:+6323676 None:intergenic
TTCTCTCGAATGACCGAAGC+CGG 0.449633 5_1:-6323430 Msa0713860:CDS
TCACGGCGTTTGCATGGTGG+AGG 0.454306 5_1:-6322620 Msa0713860:CDS
TTGATGCTTGAAAGGGAAGC+TGG 0.455749 5_1:-6322716 Msa0713860:CDS
CAGTGACGATGGTGGTTAAA+CGG 0.455896 5_1:+6323152 None:intergenic
ATGTTAGGGCTTTGTTGAGG+AGG 0.458311 5_1:-6322559 Msa0713860:CDS
TTCGGCCATGTTGCATGCAA+TGG 0.459991 5_1:-6323301 Msa0713860:CDS
TTTGTTCTATGTCACCATAA+GGG 0.462505 5_1:+6323482 None:intergenic
CTATAGCTCGTGCTGTTTCG+AGG 0.464786 5_1:+6323563 None:intergenic
CGGTGGTGTGGGCCAGTGCA+TGG 0.470323 5_1:-6322469 Msa0713860:CDS
TGAAATCAGTTGTTGGTGGA+GGG 0.471301 5_1:+6323623 None:intergenic
CATTCAGTAACAGTTGGTAA+TGG 0.475130 5_1:-6322944 Msa0713860:CDS
CATGCACTGGCCCACACCAC+CGG 0.476107 5_1:+6322470 None:intergenic
GTGAAATCAGTTGTTGGTGG+AGG 0.476494 5_1:+6323622 None:intergenic
GGAAGTTTGTGATGATGTTA+GGG 0.478484 5_1:-6322573 Msa0713860:CDS
CACGGCGTTTGCATGGTGGA+GGG 0.480736 5_1:-6322619 Msa0713860:CDS
GGTTCTGAAGGTTCTGGTAC+CGG 0.480940 5_1:+6323411 None:intergenic
AAACGCTCGTTACTCGTCTT+CGG 0.484291 5_1:+6322737 None:intergenic
TGTTGTTGTTCTGCTGGTGG+TGG 0.490954 5_1:+6323840 None:intergenic
ATGGTTTAGGGTTTACTTTG+AGG 0.492134 5_1:-6322777 Msa0713860:CDS
GATGGATATGAATTTGTGAA+TGG 0.492688 5_1:-6322818 Msa0713860:CDS
TCAAAGGTGAAATCAGTTGT+TGG 0.501730 5_1:+6323616 None:intergenic
TGTTTCGAGGAGGATGTTGT+TGG 0.505657 5_1:+6323576 None:intergenic
CCACTTTCCAGTTAATTCAA+AGG 0.510883 5_1:+6323600 None:intergenic
GAAATCAGTTGTTGGTGGAG+GGG 0.511614 5_1:+6323624 None:intergenic
GCATGGTGGAGGGTTTAATA+CGG 0.512491 5_1:-6322609 Msa0713860:CDS
CCTTTGAATTAACTGGAAAG+TGG 0.516151 5_1:-6323600 Msa0713860:CDS
TGATTTGGTGGCATGTGCTC+CGG 0.516469 5_1:-6322681 Msa0713860:CDS
TTATCAAGGCCTCCATGCAC+TGG 0.517596 5_1:+6322457 None:intergenic
GGAAGGAACAACCGGTGGTG+TGG 0.520705 5_1:-6322481 Msa0713860:CDS
GAGAGGGATAGTTGATTTGG+TGG 0.522738 5_1:-6322693 Msa0713860:CDS
CTTGACGTGGAAGGAACAAC+CGG 0.523785 5_1:-6322489 Msa0713860:CDS
AGGAAGGGTGGTCGATGATA+CGG 0.526878 5_1:-6322532 Msa0713860:CDS
CTCCGTTCAATCGACTCTGC+CGG 0.527978 5_1:+6322662 None:intergenic
ACGAGCGTTTGATGCTTGAA+AGG 0.528089 5_1:-6322724 Msa0713860:CDS
ATTCAACGTTATTCATCACT+CGG 0.529452 5_1:-6323047 Msa0713860:CDS
GAAGGAACAACCGGTGGTGT+GGG 0.535300 5_1:-6322480 Msa0713860:CDS
GTCGATTGAACGGAGAGAAA+CGG 0.535584 5_1:-6322654 Msa0713860:CDS
TCAACCACTGTTACTATCCT+CGG 0.540676 5_1:+6322869 None:intergenic
TTGTTCTATGTCACCATAAG+GGG 0.542773 5_1:+6323483 None:intergenic
CTTGAAAGGGAAGCTGGGAG+AGG 0.543069 5_1:-6322710 Msa0713860:CDS
CGGAATATTCTTGACGTGGA+AGG 0.543303 5_1:-6322498 Msa0713860:CDS
CGAGCGTTTGATGCTTGAAA+GGG 0.543796 5_1:-6322723 Msa0713860:CDS
TGATGTTGTTGTTCTGCTGG+TGG 0.544428 5_1:+6323837 None:intergenic
GGAGAGAAACGGCGGTGAGA+TGG 0.549109 5_1:-6322643 Msa0713860:CDS
TTAACCACCATCGTCACTGC+TGG 0.551969 5_1:-6323148 Msa0713860:CDS
TGTTGTTGTTGGTGTTGGTG+TGG 0.552971 5_1:+6323889 None:intergenic
GATAGTAACAGTGGTTGAAG+AGG 0.553960 5_1:-6322864 Msa0713860:CDS
TGCTGAAGCTAGGGTTCTGA+AGG 0.554762 5_1:+6323399 None:intergenic
GCTTTGCATTCAGTAACAGT+TGG 0.557029 5_1:-6322950 Msa0713860:CDS
AGATGGTCACGGCGTTTGCA+TGG 0.559260 5_1:-6322626 Msa0713860:CDS
CTTTGAATTAACTGGAAAGT+GGG 0.560664 5_1:-6323599 Msa0713860:CDS
AATTCCAAGAAGTTAGTCCA+TGG 0.567956 5_1:-6323330 Msa0713860:CDS
CAACTTAGAATCACCTTCGA+AGG 0.574160 5_1:+6323264 None:intergenic
ATGATACGGTGTAATGATGC+CGG 0.576413 5_1:-6322518 Msa0713860:CDS
ACCACCATCGTCACTGCTGG+TGG 0.581472 5_1:-6323145 Msa0713860:CDS
AGTAACAGTGGTTGAAGAGG+AGG 0.584206 5_1:-6322861 Msa0713860:CDS
CTCCGGCAGAGTCGATTGAA+CGG 0.585476 5_1:-6322664 Msa0713860:CDS
TGATGCTTGAAAGGGAAGCT+GGG 0.585745 5_1:-6322715 Msa0713860:CDS
TGTTGAGGAGGTATAAGGAA+GGG 0.588341 5_1:-6322547 Msa0713860:CDS
TTGTTGAGGAGGTATAAGGA+AGG 0.589974 5_1:-6322548 Msa0713860:CDS
CGGAGTATCATCAGCACGAG+TGG 0.595472 5_1:+6323180 None:intergenic
GTAACAGTTGGTAATGGTAA+CGG 0.598007 5_1:-6322938 Msa0713860:CDS
AAGGTGAAATCAGTTGTTGG+TGG 0.600375 5_1:+6323619 None:intergenic
TCATTCGAGAGAATAAGGCT+TGG 0.601622 5_1:+6323440 None:intergenic
TTGAAAGGGAAGCTGGGAGA+GGG 0.603420 5_1:-6322709 Msa0713860:CDS
GTTTGGAAGACGATGATGAA+AGG 0.606288 5_1:+6323719 None:intergenic
TTCAACGTTATTCATCACTC+GGG 0.607328 5_1:-6323046 Msa0713860:CDS
ATGATGTTAGGGCTTTGTTG+AGG 0.607674 5_1:-6322562 Msa0713860:CDS
CTCGTTTACAACAACTCATG+TGG 0.608017 5_1:-6323525 Msa0713860:CDS
ACATGGCCGAAAGTAGTCCA+TGG 0.613056 5_1:+6323313 None:intergenic
AAGCTATCAACGCGTCGCGA+CGG 0.617261 5_1:+6322909 None:intergenic
TGGCGCCATTGCATGCAACA+TGG 0.621243 5_1:+6323296 None:intergenic
TGAGGAGGTATAAGGAAGGG+TGG 0.622191 5_1:-6322544 Msa0713860:CDS
TAGCTTCGTTGATCGCGTTG+CGG 0.632402 5_1:-6322892 Msa0713860:CDS
TGGTCACGGCGTTTGCATGG+TGG 0.640251 5_1:-6322623 Msa0713860:CDS
AAACGGCGGTGAGATGGTCA+CGG 0.645102 5_1:-6322637 Msa0713860:CDS
TAATACGGTGGCGTTTAGTG+AGG 0.648084 5_1:-6322594 Msa0713860:CDS
AGAACCACCAGCAGTGACGA+TGG 0.652328 5_1:+6323141 None:intergenic
TGGTGGAGGGTTTAATACGG+TGG 0.662791 5_1:-6322606 Msa0713860:CDS
ATGCCGGAATATTCTTGACG+TGG 0.670161 5_1:-6322502 Msa0713860:CDS
GTAACACTTATTCCACTCAG+TGG 0.674128 5_1:-6323225 Msa0713860:CDS
ATGGTGGTTAAACGGAGGTG+CGG 0.674756 5_1:+6323160 None:intergenic
TAGCTCGTGCTGTTTCGAGG+AGG 0.677191 5_1:+6323566 None:intergenic
TGGTGTGGGCCAGTGCATGG+AGG 0.695562 5_1:-6322466 Msa0713860:CDS
GACGTGGAAGGAACAACCGG+TGG 0.699506 5_1:-6322486 Msa0713860:CDS
GCGGCCGAGGATAGTAACAG+TGG 0.704672 5_1:-6322873 Msa0713860:CDS
ACCACCAGCAGTGACGATGG+TGG 0.722652 5_1:+6323144 None:intergenic
GATTGAACGGAGAGAAACGG+CGG 0.736491 5_1:-6322651 Msa0713860:CDS
TGACGATGGTGGTTAAACGG+AGG 0.736521 5_1:+6323155 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTCTGATTTAAATTTTT+TGG - chr5_1:6323362-6323381 Msa0713860:CDS 15.0%
!!! TCTTTCTGATTTAAATTTTT+TGG - chr5_1:6323362-6323381 Msa0713860:CDS 15.0%
!! TGAATAACGTTGAATTTAAA+TGG + chr5_1:6323330-6323349 None:intergenic 20.0%
!!! GATTTAAATTTTGGTAATGA+TGG - chr5_1:6323546-6323565 Msa0713860:CDS 20.0%
!! TGAATAACGTTGAATTTAAA+TGG + chr5_1:6323330-6323349 None:intergenic 20.0%
!!! GATTTAAATTTTGGTAATGA+TGG - chr5_1:6323546-6323565 Msa0713860:CDS 20.0%
! TTCTGTTCAAAAAGTTATGA+AGG - chr5_1:6323260-6323279 Msa0713860:CDS 25.0%
! CAAAAAGTTATGAAGGAAAT+TGG - chr5_1:6323267-6323286 Msa0713860:CDS 25.0%
! TTCTGTTCAAAAAGTTATGA+AGG - chr5_1:6323260-6323279 Msa0713860:CDS 25.0%
! CAAAAAGTTATGAAGGAAAT+TGG - chr5_1:6323267-6323286 Msa0713860:CDS 25.0%
!!! TTGGTGATAGTTTTGTTTTG+AGG + chr5_1:6322584-6322603 None:intergenic 30.0%
! CTTCTTGTGGATTGTAATAT+TGG + chr5_1:6322603-6322622 None:intergenic 30.0%
!!! AGAATGCTTCAACTTTTTCA+TGG - chr5_1:6322630-6322649 Msa0713860:CDS 30.0%
ACTATAAGGATAGTAGTGTT+TGG + chr5_1:6322683-6322702 None:intergenic 30.0%
GATTTCACCTTTGAATTAAC+TGG - chr5_1:6322775-6322794 Msa0713860:CDS 30.0%
CTTTGAATTAACTGGAAAGT+GGG - chr5_1:6322783-6322802 Msa0713860:CDS 30.0%
TTTGTTCTATGTCACCATAA+GGG + chr5_1:6322903-6322922 None:intergenic 30.0%
! TTTTGTTCTATGTCACCATA+AGG + chr5_1:6322904-6322923 None:intergenic 30.0%
! GACTAACTTCTTGGAATTTT+AGG + chr5_1:6323050-6323069 None:intergenic 30.0%
GAAAAATTCGCTAGACTTAT+GGG - chr5_1:6323300-6323319 Msa0713860:CDS 30.0%
ATTCAACGTTATTCATCACT+CGG - chr5_1:6323335-6323354 Msa0713860:CDS 30.0%
GATGGATATGAATTTGTGAA+TGG - chr5_1:6323564-6323583 Msa0713860:CDS 30.0%
!!! TTGGTGATAGTTTTGTTTTG+AGG + chr5_1:6322584-6322603 None:intergenic 30.0%
! CTTCTTGTGGATTGTAATAT+TGG + chr5_1:6322603-6322622 None:intergenic 30.0%
!!! AGAATGCTTCAACTTTTTCA+TGG - chr5_1:6322630-6322649 Msa0713860:CDS 30.0%
ACTATAAGGATAGTAGTGTT+TGG + chr5_1:6322683-6322702 None:intergenic 30.0%
GATTTCACCTTTGAATTAAC+TGG - chr5_1:6322775-6322794 Msa0713860:CDS 30.0%
CTTTGAATTAACTGGAAAGT+GGG - chr5_1:6322783-6322802 Msa0713860:CDS 30.0%
TTTGTTCTATGTCACCATAA+GGG + chr5_1:6322903-6322922 None:intergenic 30.0%
! TTTTGTTCTATGTCACCATA+AGG + chr5_1:6322904-6322923 None:intergenic 30.0%
! GACTAACTTCTTGGAATTTT+AGG + chr5_1:6323050-6323069 None:intergenic 30.0%
GAAAAATTCGCTAGACTTAT+GGG - chr5_1:6323300-6323319 Msa0713860:CDS 30.0%
ATTCAACGTTATTCATCACT+CGG - chr5_1:6323335-6323354 Msa0713860:CDS 30.0%
GATGGATATGAATTTGTGAA+TGG - chr5_1:6323564-6323583 Msa0713860:CDS 30.0%
!! CTTCTTGTTGTTGTTGTTGT+TGG + chr5_1:6322507-6322526 None:intergenic 35.0%
CATTCTTCTTCAACTTCTTG+TGG + chr5_1:6322616-6322635 None:intergenic 35.0%
GTTTGTAGTAATGGTGTTTG+AGG + chr5_1:6322716-6322735 None:intergenic 35.0%
TCAAAGGTGAAATCAGTTGT+TGG + chr5_1:6322769-6322788 None:intergenic 35.0%
CCACTTTCCAGTTAATTCAA+AGG + chr5_1:6322785-6322804 None:intergenic 35.0%
CCTTTGAATTAACTGGAAAG+TGG - chr5_1:6322782-6322801 Msa0713860:CDS 35.0%
TTGTTCTATGTCACCATAAG+GGG + chr5_1:6322902-6322921 None:intergenic 35.0%
! TTTTTCTGATGCTGAAGCTA+GGG + chr5_1:6322995-6323014 None:intergenic 35.0%
!!! TTTTTTCTGATGCTGAAGCT+AGG + chr5_1:6322996-6323015 None:intergenic 35.0%
AATTCCAAGAAGTTAGTCCA+TGG - chr5_1:6323052-6323071 Msa0713860:CDS 35.0%
GGAAAAATTCGCTAGACTTA+TGG - chr5_1:6323299-6323318 Msa0713860:CDS 35.0%
TTCAACGTTATTCATCACTC+GGG - chr5_1:6323336-6323355 Msa0713860:CDS 35.0%
!! CATTCAGTAACAGTTGGTAA+TGG - chr5_1:6323438-6323457 Msa0713860:CDS 35.0%
!! GTAACAGTTGGTAATGGTAA+CGG - chr5_1:6323444-6323463 Msa0713860:CDS 35.0%
!! GAGGAGGCTGATTTAAATTT+TGG - chr5_1:6323537-6323556 Msa0713860:CDS 35.0%
!!! GTTTTGAAGAGTGTTTGAGA+TGG - chr5_1:6323586-6323605 Msa0713860:CDS 35.0%
!! AAGAGTGTTTGAGATGGTTT+AGG - chr5_1:6323592-6323611 Msa0713860:CDS 35.0%
! AGAGTGTTTGAGATGGTTTA+GGG - chr5_1:6323593-6323612 Msa0713860:CDS 35.0%
! ATGGTTTAGGGTTTACTTTG+AGG - chr5_1:6323605-6323624 Msa0713860:CDS 35.0%
! AGGAAGTTTGTGATGATGTT+AGG - chr5_1:6323808-6323827 Msa0713860:CDS 35.0%
! GGAAGTTTGTGATGATGTTA+GGG - chr5_1:6323809-6323828 Msa0713860:CDS 35.0%
!! CTTCTTGTTGTTGTTGTTGT+TGG + chr5_1:6322507-6322526 None:intergenic 35.0%
CATTCTTCTTCAACTTCTTG+TGG + chr5_1:6322616-6322635 None:intergenic 35.0%
GTTTGTAGTAATGGTGTTTG+AGG + chr5_1:6322716-6322735 None:intergenic 35.0%
TCAAAGGTGAAATCAGTTGT+TGG + chr5_1:6322769-6322788 None:intergenic 35.0%
CCACTTTCCAGTTAATTCAA+AGG + chr5_1:6322785-6322804 None:intergenic 35.0%
CCTTTGAATTAACTGGAAAG+TGG - chr5_1:6322782-6322801 Msa0713860:CDS 35.0%
TTGTTCTATGTCACCATAAG+GGG + chr5_1:6322902-6322921 None:intergenic 35.0%
! TTTTTCTGATGCTGAAGCTA+GGG + chr5_1:6322995-6323014 None:intergenic 35.0%
!!! TTTTTTCTGATGCTGAAGCT+AGG + chr5_1:6322996-6323015 None:intergenic 35.0%
AATTCCAAGAAGTTAGTCCA+TGG - chr5_1:6323052-6323071 Msa0713860:CDS 35.0%
GGAAAAATTCGCTAGACTTA+TGG - chr5_1:6323299-6323318 Msa0713860:CDS 35.0%
TTCAACGTTATTCATCACTC+GGG - chr5_1:6323336-6323355 Msa0713860:CDS 35.0%
!! CATTCAGTAACAGTTGGTAA+TGG - chr5_1:6323438-6323457 Msa0713860:CDS 35.0%
!! GTAACAGTTGGTAATGGTAA+CGG - chr5_1:6323444-6323463 Msa0713860:CDS 35.0%
!! GAGGAGGCTGATTTAAATTT+TGG - chr5_1:6323537-6323556 Msa0713860:CDS 35.0%
!!! GTTTTGAAGAGTGTTTGAGA+TGG - chr5_1:6323586-6323605 Msa0713860:CDS 35.0%
!! AAGAGTGTTTGAGATGGTTT+AGG - chr5_1:6323592-6323611 Msa0713860:CDS 35.0%
! AGAGTGTTTGAGATGGTTTA+GGG - chr5_1:6323593-6323612 Msa0713860:CDS 35.0%
! ATGGTTTAGGGTTTACTTTG+AGG - chr5_1:6323605-6323624 Msa0713860:CDS 35.0%
! AGGAAGTTTGTGATGATGTT+AGG - chr5_1:6323808-6323827 Msa0713860:CDS 35.0%
! GGAAGTTTGTGATGATGTTA+GGG - chr5_1:6323809-6323828 Msa0713860:CDS 35.0%
! GTTGTTGTTGTTGTTGGTGT+TGG + chr5_1:6322501-6322520 None:intergenic 40.0%
! ACTTGATGTTGTTGTTCTGC+TGG + chr5_1:6322551-6322570 None:intergenic 40.0%
GTTTGGAAGACGATGATGAA+AGG + chr5_1:6322666-6322685 None:intergenic 40.0%
! GAGGTTTGAGGTTGACTATA+AGG + chr5_1:6322697-6322716 None:intergenic 40.0%
! GTAATGGTGTTTGAGGTTTG+AGG + chr5_1:6322709-6322728 None:intergenic 40.0%
! TGAAATCAGTTGTTGGTGGA+GGG + chr5_1:6322762-6322781 None:intergenic 40.0%
AAGGTGAAATCAGTTGTTGG+TGG + chr5_1:6322766-6322785 None:intergenic 40.0%
CTCGTTTACAACAACTCATG+TGG - chr5_1:6322857-6322876 Msa0713860:CDS 40.0%
TCATTCGAGAGAATAAGGCT+TGG + chr5_1:6322945-6322964 None:intergenic 40.0%
TTCGGTCATTCGAGAGAATA+AGG + chr5_1:6322950-6322969 None:intergenic 40.0%
! TAGTCCATGGACTAACTTCT+TGG + chr5_1:6323059-6323078 None:intergenic 40.0%
! GTTAGTCCATGGACTACTTT+CGG - chr5_1:6323063-6323082 Msa0713860:CDS 40.0%
! ACCTTCGAAGGCTTCTAAAA+TGG + chr5_1:6323109-6323128 None:intergenic 40.0%
CAACTTAGAATCACCTTCGA+AGG + chr5_1:6323121-6323140 None:intergenic 40.0%
GTAACACTTATTCCACTCAG+TGG - chr5_1:6323157-6323176 Msa0713860:CDS 40.0%
!! GAAGGAAATTGGTGCTAGAA+TGG - chr5_1:6323278-6323297 Msa0713860:CDS 40.0%
GCTTTGCATTCAGTAACAGT+TGG - chr5_1:6323432-6323451 Msa0713860:CDS 40.0%
TCAACCACTGTTACTATCCT+CGG + chr5_1:6323516-6323535 None:intergenic 40.0%
GATAGTAACAGTGGTTGAAG+AGG - chr5_1:6323518-6323537 Msa0713860:CDS 40.0%
!! ATGATGTTAGGGCTTTGTTG+AGG - chr5_1:6323820-6323839 Msa0713860:CDS 40.0%
! TTGTTGAGGAGGTATAAGGA+AGG - chr5_1:6323834-6323853 Msa0713860:CDS 40.0%
! TGTTGAGGAGGTATAAGGAA+GGG - chr5_1:6323835-6323854 Msa0713860:CDS 40.0%
ATGATACGGTGTAATGATGC+CGG - chr5_1:6323864-6323883 Msa0713860:CDS 40.0%
CTTCCACGTCAAGAATATTC+CGG + chr5_1:6323886-6323905 None:intergenic 40.0%
! GTTGTTGTTGTTGTTGGTGT+TGG + chr5_1:6322501-6322520 None:intergenic 40.0%
! ACTTGATGTTGTTGTTCTGC+TGG + chr5_1:6322551-6322570 None:intergenic 40.0%
GTTTGGAAGACGATGATGAA+AGG + chr5_1:6322666-6322685 None:intergenic 40.0%
! GAGGTTTGAGGTTGACTATA+AGG + chr5_1:6322697-6322716 None:intergenic 40.0%
! GTAATGGTGTTTGAGGTTTG+AGG + chr5_1:6322709-6322728 None:intergenic 40.0%
! TGAAATCAGTTGTTGGTGGA+GGG + chr5_1:6322762-6322781 None:intergenic 40.0%
AAGGTGAAATCAGTTGTTGG+TGG + chr5_1:6322766-6322785 None:intergenic 40.0%
CTCGTTTACAACAACTCATG+TGG - chr5_1:6322857-6322876 Msa0713860:CDS 40.0%
TCATTCGAGAGAATAAGGCT+TGG + chr5_1:6322945-6322964 None:intergenic 40.0%
TTCGGTCATTCGAGAGAATA+AGG + chr5_1:6322950-6322969 None:intergenic 40.0%
! TAGTCCATGGACTAACTTCT+TGG + chr5_1:6323059-6323078 None:intergenic 40.0%
! GTTAGTCCATGGACTACTTT+CGG - chr5_1:6323063-6323082 Msa0713860:CDS 40.0%
! ACCTTCGAAGGCTTCTAAAA+TGG + chr5_1:6323109-6323128 None:intergenic 40.0%
CAACTTAGAATCACCTTCGA+AGG + chr5_1:6323121-6323140 None:intergenic 40.0%
GTAACACTTATTCCACTCAG+TGG - chr5_1:6323157-6323176 Msa0713860:CDS 40.0%
!! GAAGGAAATTGGTGCTAGAA+TGG - chr5_1:6323278-6323297 Msa0713860:CDS 40.0%
GCTTTGCATTCAGTAACAGT+TGG - chr5_1:6323432-6323451 Msa0713860:CDS 40.0%
TCAACCACTGTTACTATCCT+CGG + chr5_1:6323516-6323535 None:intergenic 40.0%
GATAGTAACAGTGGTTGAAG+AGG - chr5_1:6323518-6323537 Msa0713860:CDS 40.0%
!! ATGATGTTAGGGCTTTGTTG+AGG - chr5_1:6323820-6323839 Msa0713860:CDS 40.0%
! TTGTTGAGGAGGTATAAGGA+AGG - chr5_1:6323834-6323853 Msa0713860:CDS 40.0%
! TGTTGAGGAGGTATAAGGAA+GGG - chr5_1:6323835-6323854 Msa0713860:CDS 40.0%
ATGATACGGTGTAATGATGC+CGG - chr5_1:6323864-6323883 Msa0713860:CDS 40.0%
CTTCCACGTCAAGAATATTC+CGG + chr5_1:6323886-6323905 None:intergenic 40.0%
!! TGTTGTTGTTGGTGTTGGTG+TGG + chr5_1:6322496-6322515 None:intergenic 45.0%
! TGATGTTGTTGTTCTGCTGG+TGG + chr5_1:6322548-6322567 None:intergenic 45.0%
!! TGTGGGGGTGTTTGTAGTAA+TGG + chr5_1:6322725-6322744 None:intergenic 45.0%
! AAATCAGTTGTTGGTGGAGG+GGG + chr5_1:6322760-6322779 None:intergenic 45.0%
! GAAATCAGTTGTTGGTGGAG+GGG + chr5_1:6322761-6322780 None:intergenic 45.0%
! GTGAAATCAGTTGTTGGTGG+AGG + chr5_1:6322763-6322782 None:intergenic 45.0%
TGTTTCGAGGAGGATGTTGT+TGG + chr5_1:6322809-6322828 None:intergenic 45.0%
AACGAACTAAGCTCCCCTTA+TGG - chr5_1:6322886-6322905 Msa0713860:CDS 45.0%
!! GCCATTTTAGAAGCCTTCGA+AGG - chr5_1:6323105-6323124 Msa0713860:CDS 45.0%
! CAGTGACGATGGTGGTTAAA+CGG + chr5_1:6323233-6323252 None:intergenic 45.0%
AGTAACAGTGGTTGAAGAGG+AGG - chr5_1:6323521-6323540 Msa0713860:CDS 45.0%
AAACGCTCGTTACTCGTCTT+CGG + chr5_1:6323648-6323667 None:intergenic 45.0%
ACGAGCGTTTGATGCTTGAA+AGG - chr5_1:6323658-6323677 Msa0713860:CDS 45.0%
CGAGCGTTTGATGCTTGAAA+GGG - chr5_1:6323659-6323678 Msa0713860:CDS 45.0%
TTGATGCTTGAAAGGGAAGC+TGG - chr5_1:6323666-6323685 Msa0713860:CDS 45.0%
TGATGCTTGAAAGGGAAGCT+GGG - chr5_1:6323667-6323686 Msa0713860:CDS 45.0%
TGGGAGAGGGATAGTTGATT+TGG - chr5_1:6323686-6323705 Msa0713860:CDS 45.0%
!! GAGAGGGATAGTTGATTTGG+TGG - chr5_1:6323689-6323708 Msa0713860:CDS 45.0%
GTCGATTGAACGGAGAGAAA+CGG - chr5_1:6323728-6323747 Msa0713860:CDS 45.0%
! GCATGGTGGAGGGTTTAATA+CGG - chr5_1:6323773-6323792 Msa0713860:CDS 45.0%
TAATACGGTGGCGTTTAGTG+AGG - chr5_1:6323788-6323807 Msa0713860:CDS 45.0%
!! ATGTTAGGGCTTTGTTGAGG+AGG - chr5_1:6323823-6323842 Msa0713860:CDS 45.0%
!! GGCTTTGTTGAGGAGGTATA+AGG - chr5_1:6323830-6323849 Msa0713860:CDS 45.0%
ATGCCGGAATATTCTTGACG+TGG - chr5_1:6323880-6323899 Msa0713860:CDS 45.0%
CGGAATATTCTTGACGTGGA+AGG - chr5_1:6323884-6323903 Msa0713860:CDS 45.0%
!! TGTTGTTGTTGGTGTTGGTG+TGG + chr5_1:6322496-6322515 None:intergenic 45.0%
! TGATGTTGTTGTTCTGCTGG+TGG + chr5_1:6322548-6322567 None:intergenic 45.0%
!! TGTGGGGGTGTTTGTAGTAA+TGG + chr5_1:6322725-6322744 None:intergenic 45.0%
! AAATCAGTTGTTGGTGGAGG+GGG + chr5_1:6322760-6322779 None:intergenic 45.0%
! GAAATCAGTTGTTGGTGGAG+GGG + chr5_1:6322761-6322780 None:intergenic 45.0%
! GTGAAATCAGTTGTTGGTGG+AGG + chr5_1:6322763-6322782 None:intergenic 45.0%
TGTTTCGAGGAGGATGTTGT+TGG + chr5_1:6322809-6322828 None:intergenic 45.0%
AACGAACTAAGCTCCCCTTA+TGG - chr5_1:6322886-6322905 Msa0713860:CDS 45.0%
!! GCCATTTTAGAAGCCTTCGA+AGG - chr5_1:6323105-6323124 Msa0713860:CDS 45.0%
! CAGTGACGATGGTGGTTAAA+CGG + chr5_1:6323233-6323252 None:intergenic 45.0%
AGTAACAGTGGTTGAAGAGG+AGG - chr5_1:6323521-6323540 Msa0713860:CDS 45.0%
AAACGCTCGTTACTCGTCTT+CGG + chr5_1:6323648-6323667 None:intergenic 45.0%
ACGAGCGTTTGATGCTTGAA+AGG - chr5_1:6323658-6323677 Msa0713860:CDS 45.0%
CGAGCGTTTGATGCTTGAAA+GGG - chr5_1:6323659-6323678 Msa0713860:CDS 45.0%
TTGATGCTTGAAAGGGAAGC+TGG - chr5_1:6323666-6323685 Msa0713860:CDS 45.0%
TGATGCTTGAAAGGGAAGCT+GGG - chr5_1:6323667-6323686 Msa0713860:CDS 45.0%
TGGGAGAGGGATAGTTGATT+TGG - chr5_1:6323686-6323705 Msa0713860:CDS 45.0%
!! GAGAGGGATAGTTGATTTGG+TGG - chr5_1:6323689-6323708 Msa0713860:CDS 45.0%
GTCGATTGAACGGAGAGAAA+CGG - chr5_1:6323728-6323747 Msa0713860:CDS 45.0%
! GCATGGTGGAGGGTTTAATA+CGG - chr5_1:6323773-6323792 Msa0713860:CDS 45.0%
TAATACGGTGGCGTTTAGTG+AGG - chr5_1:6323788-6323807 Msa0713860:CDS 45.0%
!! ATGTTAGGGCTTTGTTGAGG+AGG - chr5_1:6323823-6323842 Msa0713860:CDS 45.0%
!! GGCTTTGTTGAGGAGGTATA+AGG - chr5_1:6323830-6323849 Msa0713860:CDS 45.0%
ATGCCGGAATATTCTTGACG+TGG - chr5_1:6323880-6323899 Msa0713860:CDS 45.0%
CGGAATATTCTTGACGTGGA+AGG - chr5_1:6323884-6323903 Msa0713860:CDS 45.0%
!! GTTGTTGGTGTTGGTGTGGT+TGG + chr5_1:6322492-6322511 None:intergenic 50.0%
! TGTTGTTGTTCTGCTGGTGG+TGG + chr5_1:6322545-6322564 None:intergenic 50.0%
!! CTATAGCTCGTGCTGTTTCG+AGG + chr5_1:6322822-6322841 None:intergenic 50.0%
TTCTCTCGAATGACCGAAGC+CGG - chr5_1:6322952-6322971 Msa0713860:CDS 50.0%
!! GGTTCTGAAGGTTCTGGTAC+CGG + chr5_1:6322974-6322993 None:intergenic 50.0%
!! AGCTAGGGTTCTGAAGGTTC+TGG + chr5_1:6322980-6322999 None:intergenic 50.0%
! TGCTGAAGCTAGGGTTCTGA+AGG + chr5_1:6322986-6323005 None:intergenic 50.0%
ACATGGCCGAAAGTAGTCCA+TGG + chr5_1:6323072-6323091 None:intergenic 50.0%
TTCGGCCATGTTGCATGCAA+TGG - chr5_1:6323081-6323100 Msa0713860:CDS 50.0%
!! AAAAAGCGTTGGCCACTGAG+TGG + chr5_1:6323172-6323191 None:intergenic 50.0%
!!! GCCAACGCTTTTTGAAGCGT+TGG - chr5_1:6323179-6323198 Msa0713860:CDS 50.0%
! GCCAACGCTTCAAAAAGCGT+TGG + chr5_1:6323183-6323202 None:intergenic 50.0%
ATGGTGGTTAAACGGAGGTG+CGG + chr5_1:6323225-6323244 None:intergenic 50.0%
! TGACGATGGTGGTTAAACGG+AGG + chr5_1:6323230-6323249 None:intergenic 50.0%
TTAACCACCATCGTCACTGC+TGG - chr5_1:6323234-6323253 Msa0713860:CDS 50.0%
TAGCTTCGTTGATCGCGTTG+CGG - chr5_1:6323490-6323509 Msa0713860:CDS 50.0%
TTGAAAGGGAAGCTGGGAGA+GGG - chr5_1:6323673-6323692 Msa0713860:CDS 50.0%
!! TGATTTGGTGGCATGTGCTC+CGG - chr5_1:6323701-6323720 Msa0713860:CDS 50.0%
GATTGAACGGAGAGAAACGG+CGG - chr5_1:6323731-6323750 Msa0713860:CDS 50.0%
TGGTGGAGGGTTTAATACGG+TGG - chr5_1:6323776-6323795 Msa0713860:CDS 50.0%
! TGAGGAGGTATAAGGAAGGG+TGG - chr5_1:6323838-6323857 Msa0713860:CDS 50.0%
AGGAAGGGTGGTCGATGATA+CGG - chr5_1:6323850-6323869 Msa0713860:CDS 50.0%
CTTGACGTGGAAGGAACAAC+CGG - chr5_1:6323893-6323912 Msa0713860:CDS 50.0%
!! GTTGTTGGTGTTGGTGTGGT+TGG + chr5_1:6322492-6322511 None:intergenic 50.0%
! TGTTGTTGTTCTGCTGGTGG+TGG + chr5_1:6322545-6322564 None:intergenic 50.0%
!! CTATAGCTCGTGCTGTTTCG+AGG + chr5_1:6322822-6322841 None:intergenic 50.0%
TTCTCTCGAATGACCGAAGC+CGG - chr5_1:6322952-6322971 Msa0713860:CDS 50.0%
!! GGTTCTGAAGGTTCTGGTAC+CGG + chr5_1:6322974-6322993 None:intergenic 50.0%
!! AGCTAGGGTTCTGAAGGTTC+TGG + chr5_1:6322980-6322999 None:intergenic 50.0%
! TGCTGAAGCTAGGGTTCTGA+AGG + chr5_1:6322986-6323005 None:intergenic 50.0%
ACATGGCCGAAAGTAGTCCA+TGG + chr5_1:6323072-6323091 None:intergenic 50.0%
TTCGGCCATGTTGCATGCAA+TGG - chr5_1:6323081-6323100 Msa0713860:CDS 50.0%
!! AAAAAGCGTTGGCCACTGAG+TGG + chr5_1:6323172-6323191 None:intergenic 50.0%
!!! GCCAACGCTTTTTGAAGCGT+TGG - chr5_1:6323179-6323198 Msa0713860:CDS 50.0%
! GCCAACGCTTCAAAAAGCGT+TGG + chr5_1:6323183-6323202 None:intergenic 50.0%
ATGGTGGTTAAACGGAGGTG+CGG + chr5_1:6323225-6323244 None:intergenic 50.0%
! TGACGATGGTGGTTAAACGG+AGG + chr5_1:6323230-6323249 None:intergenic 50.0%
TTAACCACCATCGTCACTGC+TGG - chr5_1:6323234-6323253 Msa0713860:CDS 50.0%
TAGCTTCGTTGATCGCGTTG+CGG - chr5_1:6323490-6323509 Msa0713860:CDS 50.0%
TTGAAAGGGAAGCTGGGAGA+GGG - chr5_1:6323673-6323692 Msa0713860:CDS 50.0%
!! TGATTTGGTGGCATGTGCTC+CGG - chr5_1:6323701-6323720 Msa0713860:CDS 50.0%
GATTGAACGGAGAGAAACGG+CGG - chr5_1:6323731-6323750 Msa0713860:CDS 50.0%
TGGTGGAGGGTTTAATACGG+TGG - chr5_1:6323776-6323795 Msa0713860:CDS 50.0%
! TGAGGAGGTATAAGGAAGGG+TGG - chr5_1:6323838-6323857 Msa0713860:CDS 50.0%
AGGAAGGGTGGTCGATGATA+CGG - chr5_1:6323850-6323869 Msa0713860:CDS 50.0%
CTTGACGTGGAAGGAACAAC+CGG - chr5_1:6323893-6323912 Msa0713860:CDS 50.0%
!! TAGCTCGTGCTGTTTCGAGG+AGG + chr5_1:6322819-6322838 None:intergenic 55.0%
!! GAAGGTTCTGGTACCGGCTT+CGG + chr5_1:6322968-6322987 None:intergenic 55.0%
TGGCGCCATTGCATGCAACA+TGG + chr5_1:6323089-6323108 None:intergenic 55.0%
! CGGAGTATCATCAGCACGAG+TGG + chr5_1:6323205-6323224 None:intergenic 55.0%
AGAACCACCAGCAGTGACGA+TGG + chr5_1:6323244-6323263 None:intergenic 55.0%
AAGCTATCAACGCGTCGCGA+CGG + chr5_1:6323476-6323495 None:intergenic 55.0%
CTTGAAAGGGAAGCTGGGAG+AGG - chr5_1:6323672-6323691 Msa0713860:CDS 55.0%
CTCCGGCAGAGTCGATTGAA+CGG - chr5_1:6323718-6323737 Msa0713860:CDS 55.0%
! CTCCGTTCAATCGACTCTGC+CGG + chr5_1:6323723-6323742 None:intergenic 55.0%
AAACGGCGGTGAGATGGTCA+CGG - chr5_1:6323745-6323764 Msa0713860:CDS 55.0%
AGATGGTCACGGCGTTTGCA+TGG - chr5_1:6323756-6323775 Msa0713860:CDS 55.0%
GAAGGAACAACCGGTGGTGT+GGG - chr5_1:6323902-6323921 Msa0713860:CDS 55.0%
!! TAGCTCGTGCTGTTTCGAGG+AGG + chr5_1:6322819-6322838 None:intergenic 55.0%
!! GAAGGTTCTGGTACCGGCTT+CGG + chr5_1:6322968-6322987 None:intergenic 55.0%
TGGCGCCATTGCATGCAACA+TGG + chr5_1:6323089-6323108 None:intergenic 55.0%
! CGGAGTATCATCAGCACGAG+TGG + chr5_1:6323205-6323224 None:intergenic 55.0%
AGAACCACCAGCAGTGACGA+TGG + chr5_1:6323244-6323263 None:intergenic 55.0%
AAGCTATCAACGCGTCGCGA+CGG + chr5_1:6323476-6323495 None:intergenic 55.0%
CTTGAAAGGGAAGCTGGGAG+AGG - chr5_1:6323672-6323691 Msa0713860:CDS 55.0%
CTCCGGCAGAGTCGATTGAA+CGG - chr5_1:6323718-6323737 Msa0713860:CDS 55.0%
! CTCCGTTCAATCGACTCTGC+CGG + chr5_1:6323723-6323742 None:intergenic 55.0%
AAACGGCGGTGAGATGGTCA+CGG - chr5_1:6323745-6323764 Msa0713860:CDS 55.0%
AGATGGTCACGGCGTTTGCA+TGG - chr5_1:6323756-6323775 Msa0713860:CDS 55.0%
GAAGGAACAACCGGTGGTGT+GGG - chr5_1:6323902-6323921 Msa0713860:CDS 55.0%
! GGGTGTGAGTGATGGTGTGG+GGG + chr5_1:6322740-6322759 None:intergenic 60.0%
! GGGGTGTGAGTGATGGTGTG+GGG + chr5_1:6322741-6322760 None:intergenic 60.0%
! GGGGGTGTGAGTGATGGTGT+GGG + chr5_1:6322742-6322761 None:intergenic 60.0%
! AGGGGGTGTGAGTGATGGTG+TGG + chr5_1:6322743-6322762 None:intergenic 60.0%
ACCACCATCGTCACTGCTGG+TGG - chr5_1:6323237-6323256 Msa0713860:CDS 60.0%
ACCACCAGCAGTGACGATGG+TGG + chr5_1:6323241-6323260 None:intergenic 60.0%
GCGGCCGAGGATAGTAACAG+TGG - chr5_1:6323509-6323528 Msa0713860:CDS 60.0%
GGAGAGAAACGGCGGTGAGA+TGG - chr5_1:6323739-6323758 Msa0713860:CDS 60.0%
TGGTCACGGCGTTTGCATGG+TGG - chr5_1:6323759-6323778 Msa0713860:CDS 60.0%
TCACGGCGTTTGCATGGTGG+AGG - chr5_1:6323762-6323781 Msa0713860:CDS 60.0%
! CACGGCGTTTGCATGGTGGA+GGG - chr5_1:6323763-6323782 Msa0713860:CDS 60.0%
GACGTGGAAGGAACAACCGG+TGG - chr5_1:6323896-6323915 Msa0713860:CDS 60.0%
GGAAGGAACAACCGGTGGTG+TGG - chr5_1:6323901-6323920 Msa0713860:CDS 60.0%
! GGGTGTGAGTGATGGTGTGG+GGG + chr5_1:6322740-6322759 None:intergenic 60.0%
! GGGGTGTGAGTGATGGTGTG+GGG + chr5_1:6322741-6322760 None:intergenic 60.0%
! GGGGGTGTGAGTGATGGTGT+GGG + chr5_1:6322742-6322761 None:intergenic 60.0%
! AGGGGGTGTGAGTGATGGTG+TGG + chr5_1:6322743-6322762 None:intergenic 60.0%
ACCACCATCGTCACTGCTGG+TGG - chr5_1:6323237-6323256 Msa0713860:CDS 60.0%
ACCACCAGCAGTGACGATGG+TGG + chr5_1:6323241-6323260 None:intergenic 60.0%
GCGGCCGAGGATAGTAACAG+TGG - chr5_1:6323509-6323528 Msa0713860:CDS 60.0%
GGAGAGAAACGGCGGTGAGA+TGG - chr5_1:6323739-6323758 Msa0713860:CDS 60.0%
TGGTCACGGCGTTTGCATGG+TGG - chr5_1:6323759-6323778 Msa0713860:CDS 60.0%
TCACGGCGTTTGCATGGTGG+AGG - chr5_1:6323762-6323781 Msa0713860:CDS 60.0%
! CACGGCGTTTGCATGGTGGA+GGG - chr5_1:6323763-6323782 Msa0713860:CDS 60.0%
GACGTGGAAGGAACAACCGG+TGG - chr5_1:6323896-6323915 Msa0713860:CDS 60.0%
GGAAGGAACAACCGGTGGTG+TGG - chr5_1:6323901-6323920 Msa0713860:CDS 60.0%
! GGTGGAGGGGGTGTGAGTGA+TGG + chr5_1:6322748-6322767 None:intergenic 65.0%
! CATGCACTGGCCCACACCAC+CGG + chr5_1:6323915-6323934 None:intergenic 65.0%
TGGTGTGGGCCAGTGCATGG+AGG - chr5_1:6323916-6323935 Msa0713860:CDS 65.0%
! GGTGGAGGGGGTGTGAGTGA+TGG + chr5_1:6322748-6322767 None:intergenic 65.0%
! CATGCACTGGCCCACACCAC+CGG + chr5_1:6323915-6323934 None:intergenic 65.0%
TGGTGTGGGCCAGTGCATGG+AGG - chr5_1:6323916-6323935 Msa0713860:CDS 65.0%
CGTTGATCGCGTTGCGGCCG+AGG - chr5_1:6323496-6323515 Msa0713860:CDS 70.0%
CGGTGGTGTGGGCCAGTGCA+TGG - chr5_1:6323913-6323932 Msa0713860:CDS 70.0%
CGTTGATCGCGTTGCGGCCG+AGG - chr5_1:6323496-6323515 Msa0713860:CDS 70.0%
CGGTGGTGTGGGCCAGTGCA+TGG - chr5_1:6323913-6323932 Msa0713860:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 6322460 6323944 6322460 ID=Msa0713860;Name=Msa0713860
chr5_1 mRNA 6322460 6323944 6322460 ID=Msa0713860-mRNA-1;Parent=Msa0713860;Name=Msa0713860-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|494
chr5_1 exon 6322460 6323944 6322460 ID=Msa0713860-mRNA-1:exon:2666;Parent=Msa0713860-mRNA-1
chr5_1 CDS 6322460 6323944 6322460 ID=Msa0713860-mRNA-1:cds;Parent=Msa0713860-mRNA-1
Gene Sequence

>Msa0713860

ATGGATACATTGTTTAGACTTGTAAGCTTCCAACCACACCAACACCAACAACAACAACAACAAGAAGATCAATCTCTCAACTCCACCACCAGCAGAACAACAACATCAAGTAGCTCTCGATCCTCAAAACAAAACTATCACCAATATTACAATCCACAAGAAGTTGAAGAAGAATGCTTCAACTTTTTCATGGATGAAGAAGACCTTTCATCATCGTCTTCCAAACACTACTATCCTTATAGTCAACCTCAAACCTCAAACACCATTACTACAAACACCCCCACACCATCACTCACACCCCCTCCACCAACAACTGATTTCACCTTTGAATTAACTGGAAAGTGGGCCAACAACATCCTCCTCGAAACAGCACGAGCTATAGCAGAAAAAAACAGCACTCGTTTACAACAACTCATGTGGATGCTCAACGAACTAAGCTCCCCTTATGGTGACATAGAACAAAAACTAGCTGCATATTTTCTCCAAGCCTTATTCTCTCGAATGACCGAAGCCGGTACCAGAACCTTCAGAACCCTAGCTTCAGCATCAGAAAAAACTTGCTCATTCGAATCAACACGAAAAACAGTCCTAAAATTCCAAGAAGTTAGTCCATGGACTACTTTCGGCCATGTTGCATGCAATGGCGCCATTTTAGAAGCCTTCGAAGGTGATTCTAAGTTGCACATAATTGATATTAGTAACACTTATTCCACTCAGTGGCCAACGCTTTTTGAAGCGTTGGCCACTCGTGCTGATGATACTCCGCACCTCCGTTTAACCACCATCGTCACTGCTGGTGGTTCTGTTCAAAAAGTTATGAAGGAAATTGGTGCTAGAATGGAAAAATTCGCTAGACTTATGGGAGTTCCATTTAAATTCAACGTTATTCATCACTCGGGCGATCTTTCTGATTTAAATTTTTTGGATTTAGATATTAAAGAAGATGAAGCTTTAGCTGTTAATTGTGTTAATGCTTTGCATTCAGTAACAGTTGGTAATGGTAACGGTAATAACCGTCGCGACGCGTTGATAGCTTCGTTGATCGCGTTGCGGCCGAGGATAGTAACAGTGGTTGAAGAGGAGGCTGATTTAAATTTTGGTAATGATGGATATGAATTTGTGAATGGTTTTGAAGAGTGTTTGAGATGGTTTAGGGTTTACTTTGAGGCATTAGAAGAGAGTTTTCCGAAGACGAGTAACGAGCGTTTGATGCTTGAAAGGGAAGCTGGGAGAGGGATAGTTGATTTGGTGGCATGTGCTCCGGCAGAGTCGATTGAACGGAGAGAAACGGCGGTGAGATGGTCACGGCGTTTGCATGGTGGAGGGTTTAATACGGTGGCGTTTAGTGAGGAAGTTTGTGATGATGTTAGGGCTTTGTTGAGGAGGTATAAGGAAGGGTGGTCGATGATACGGTGTAATGATGCCGGAATATTCTTGACGTGGAAGGAACAACCGGTGGTGTGGGCCAGTGCATGGAGGCCTTGA
Protein sequence

>Msa0713860

MDTLFRLVSFQPHQHQQQQQQEDQSLNSTTSRTTTSSSSRSSKQNYHQYYNPQEVEEECFNFFMDEEDLSSSSSKHYYPYSQPQTSNTITTNTPTPSLTPPPPTTDFTFELTGKWANNILLETARAIAEKNSTRLQQLMWMLNELSSPYGDIEQKLAAYFLQALFSRMTEAGTRTFRTLASASEKTCSFESTRKTVLKFQEVSPWTTFGHVACNGAILEAFEGDSKLHIIDISNTYSTQWPTLFEALATRADDTPHLRLTTIVTAGGSVQKVMKEIGARMEKFARLMGVPFKFNVIHHSGDLSDLNFLDLDIKEDEALAVNCVNALHSVTVGNGNGNNRRDALIASLIALRPRIVTVVEEEADLNFGNDGYEFVNGFEECLRWFRVYFEALEESFPKTSNERLMLEREAGRGIVDLVACAPAESIERRETAVRWSRRLHGGGFNTVAFSEEVCDDVRALLRRYKEGWSMIRCNDAGIFLTWKEQPVVWASAWRP*