AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0835240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835240 MtrunA17_Chr5g0401131 96.177 497 11 2 1 495 1 491 0.0 988
Msa0835240 MtrunA17_Chr2g0329591 54.293 396 156 7 114 495 68 452 8.10e-144 420
Msa0835240 MtrunA17_Chr4g0054071 60.569 246 74 7 1 228 1 241 1.86e-69 222
Msa0835240 MtrunA17_Chr5g0447691 30.000 390 252 7 112 494 169 544 5.71e-56 195
Msa0835240 MtrunA17_Chr2g0319091 32.105 380 239 7 120 493 212 578 6.73e-56 196
Msa0835240 MtrunA17_Chr1g0159461 31.316 380 244 8 120 494 225 592 1.32e-55 195
Msa0835240 MtrunA17_Chr5g0445331 30.809 383 246 7 120 494 161 532 2.94e-54 190
Msa0835240 MtrunA17_Chr3g0125811 31.316 380 244 8 120 494 203 570 1.11e-51 184
Msa0835240 MtrunA17_Chr3g0101141 29.630 405 239 12 115 494 159 542 6.68e-51 181
Msa0835240 MtrunA17_Chr4g0053901 28.446 457 280 10 81 495 18 469 8.15e-50 177
Msa0835240 MtrunA17_Chr7g0224271 31.436 404 232 13 113 494 293 673 6.07e-48 176
Msa0835240 MtrunA17_Chr5g0401421 28.217 443 258 12 100 495 18 447 1.32e-46 168
Msa0835240 MtrunA17_Chr2g0290241 29.444 360 233 8 142 494 80 425 2.06e-46 167
Msa0835240 MtrunA17_Chr4g0076091 29.167 384 246 10 120 493 175 542 1.20e-44 164
Msa0835240 MtrunA17_Chr3g0110971 28.017 464 280 10 62 494 147 587 2.77e-44 164
Msa0835240 MtrunA17_Chr8g0344101 31.188 404 235 13 114 494 217 600 2.41e-43 162
Msa0835240 MtrunA17_Chr8g0376381 30.526 380 231 10 121 494 170 522 7.80e-43 159
Msa0835240 MtrunA17_Chr8g0357601 29.474 380 252 9 121 494 183 552 8.14e-42 157
Msa0835240 MtrunA17_Chr7g0245601 28.836 378 239 14 123 495 441 793 1.02e-41 159
Msa0835240 MtrunA17_Chr2g0306641 27.236 492 303 15 21 494 33 487 3.58e-41 154
Msa0835240 MtrunA17_Chr4g0057291 27.720 386 257 11 119 495 291 663 1.22e-40 155
Msa0835240 MtrunA17_Chr4g0031741 28.796 382 252 9 120 495 284 651 8.99e-40 152
Msa0835240 MtrunA17_Chr4g0038761 28.609 381 240 15 123 495 87 443 1.99e-38 145
Msa0835240 MtrunA17_Chr4g0031711 26.842 380 270 4 119 495 360 734 3.37e-38 148
Msa0835240 MtrunA17_Chr4g0039661 29.870 385 236 11 121 494 194 555 1.21e-37 145
Msa0835240 MtrunA17_Chr5g0396491 25.755 497 281 13 64 494 4 478 2.98e-37 143
Msa0835240 MtrunA17_Chr2g0328341 26.316 380 264 9 120 495 272 639 6.29e-37 144
Msa0835240 MtrunA17_Chr2g0328391 28.272 382 258 7 120 495 299 670 2.35e-36 142
Msa0835240 MtrunA17_Chr4g0031701 27.920 351 241 7 119 464 253 596 6.14e-36 141
Msa0835240 MtrunA17_Chr7g0238891 26.302 384 261 8 120 495 372 741 5.96e-35 139
Msa0835240 MtrunA17_Chr4g0031731 27.920 351 241 7 119 464 253 596 2.02e-34 136
Msa0835240 MtrunA17_Chr4g0031721 26.649 379 266 5 120 495 316 685 2.35e-34 137
Msa0835240 MtrunA17_Chr4g0057891 25.964 389 257 10 120 494 149 520 2.50e-34 135
Msa0835240 MtrunA17_Chr2g0328381 25.789 380 266 9 120 495 275 642 3.20e-34 136
Msa0835240 MtrunA17_Chr2g0328411 25.464 377 266 6 120 493 289 653 2.96e-33 133
Msa0835240 MtrunA17_Chr2g0328431 25.654 382 263 7 120 495 289 655 2.75e-32 130
Msa0835240 MtrunA17_Chr3g0085681 23.765 425 259 9 121 494 26 436 9.78e-32 126
Msa0835240 MtrunA17_Chr2g0328401 26.934 349 236 7 120 463 290 624 1.52e-30 125
Msa0835240 MtrunA17_Chr1g0183641 27.105 380 245 12 120 493 123 476 1.79e-30 124
Msa0835240 MtrunA17_Chr3g0114841 27.654 405 239 15 119 493 119 499 1.27e-27 115
Msa0835240 MtrunA17_Chr2g0328361 23.810 378 251 8 120 495 274 616 4.18e-27 115
Msa0835240 MtrunA17_Chr4g0038851 30.364 247 162 4 100 345 28 265 1.55e-26 112
Msa0835240 MtrunA17_Chr1g0200401 27.094 406 241 14 119 494 79 459 2.90e-26 111
Msa0835240 MtrunA17_Chr8g0383981 26.804 388 245 17 119 495 138 497 1.16e-24 107
Msa0835240 MtrunA17_Chr2g0294471 25.831 391 254 14 121 494 205 576 1.89e-24 106
Msa0835240 MtrunA17_Chr5g0424131 27.778 396 248 14 119 493 120 498 4.35e-24 105
Msa0835240 MtrunA17_Chr2g0294461 24.416 385 270 8 121 494 211 585 8.98e-24 104
Msa0835240 MtrunA17_Chr7g0236851 24.816 407 279 9 98 493 266 656 4.41e-23 102
Msa0835240 MtrunA17_Chr2g0323111 32.418 182 117 3 315 495 131 307 8.18e-23 99.0
Msa0835240 MtrunA17_Chr3g0085361 23.478 345 202 8 200 494 18 350 8.27e-23 99.8
Msa0835240 MtrunA17_Chr6g0468561 26.166 386 254 11 121 494 296 662 4.48e-22 99.8
Msa0835240 MtrunA17_Chr7g0270031 24.202 376 272 6 121 494 190 554 1.27e-20 95.1
Msa0835240 MtrunA17_Chr1g0194621 23.611 360 256 7 142 494 171 518 1.58e-19 91.7
Msa0835240 MtrunA17_Chr2g0328371 25.703 249 175 6 200 446 8 248 1.02e-18 85.9
Msa0835240 MtrunA17_Chr4g0012071 21.751 377 270 8 121 494 197 551 1.81e-16 82.0
Msa0835240 MtrunA17_Chr7g0230771 21.628 393 252 9 121 494 376 731 5.40e-15 77.8
Msa0835240 MtrunA17_Chr7g0230731 21.120 393 254 9 121 494 373 728 3.34e-14 75.5
Msa0835240 MtrunA17_Chr8g0373561 23.291 395 265 11 107 494 178 541 2.03e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835240 AT4G37650.1 58.487 542 166 11 1 495 1 530 0.0 577
Msa0835240 AT2G04890.1 31.649 376 241 7 120 494 53 413 9.80e-60 202
Msa0835240 AT1G21450.1 32.987 385 232 9 120 494 225 593 4.12e-52 186
Msa0835240 AT5G48150.1 28.647 377 257 6 121 494 123 490 1.00e-51 183
Msa0835240 AT5G48150.4 28.647 377 257 6 121 494 123 490 1.00e-51 183
Msa0835240 AT5G48150.2 28.647 377 257 6 121 494 123 490 1.00e-51 183
Msa0835240 AT5G48150.3 28.647 377 257 6 121 494 123 490 1.00e-51 183
Msa0835240 AT1G50600.3 30.390 385 243 9 119 494 158 526 8.62e-51 181
Msa0835240 AT1G50600.1 30.390 385 243 9 119 494 229 597 1.94e-50 182
Msa0835240 AT1G50600.2 30.390 385 243 9 119 494 233 601 2.41e-50 181
Msa0835240 AT3G49950.1 32.308 390 240 10 120 495 30 409 1.12e-49 176
Msa0835240 AT3G03450.1 30.052 386 250 7 113 494 176 545 5.36e-47 171
Msa0835240 AT1G66350.1 30.394 431 270 12 67 494 103 506 1.88e-46 169
Msa0835240 AT5G17490.1 31.266 387 244 8 110 494 150 516 2.03e-46 169
Msa0835240 AT2G01570.1 29.178 377 244 10 121 494 225 581 6.24e-42 158
Msa0835240 AT4G17230.1 26.804 388 253 9 120 495 158 526 2.53e-41 155
Msa0835240 AT4G17230.2 26.804 388 253 9 120 495 158 526 2.53e-41 155
Msa0835240 AT1G50420.1 25.745 470 279 7 85 494 20 479 4.26e-41 154
Msa0835240 AT1G14920.1 29.443 377 243 10 121 494 173 529 2.65e-40 152
Msa0835240 AT3G13840.1 30.982 397 230 14 114 494 142 510 6.81e-40 151
Msa0835240 AT3G54220.1 29.581 382 234 16 123 495 296 651 9.58e-39 150
Msa0835240 AT1G63100.2 29.156 391 248 11 119 493 276 653 2.08e-36 143
Msa0835240 AT1G63100.1 29.156 391 248 11 119 493 251 628 2.26e-36 142
Msa0835240 AT1G55580.1 28.883 412 246 12 120 494 44 445 3.43e-35 137
Msa0835240 AT5G41920.1 29.243 383 240 14 119 494 42 400 4.06e-35 135
Msa0835240 AT5G66770.1 28.457 376 242 10 128 494 227 584 3.73e-34 135
Msa0835240 AT3G46600.2 27.559 381 261 8 119 495 3 372 5.33e-34 132
Msa0835240 AT2G37650.2 26.893 383 260 8 119 495 334 702 1.32e-33 135
Msa0835240 AT2G37650.1 26.893 383 260 8 119 495 346 714 1.50e-33 135
Msa0835240 AT5G52510.1 27.551 392 246 9 121 494 269 640 1.68e-33 134
Msa0835240 AT3G46600.1 27.559 381 261 8 119 495 211 580 2.36e-33 133
Msa0835240 AT2G29065.1 26.357 387 265 10 120 495 252 629 2.77e-33 134
Msa0835240 AT3G46600.3 27.559 381 261 8 119 495 179 548 3.48e-33 132
Msa0835240 AT1G07530.1 25.263 380 271 6 120 495 396 766 2.79e-32 131
Msa0835240 AT5G59450.1 26.178 382 269 6 119 495 226 599 4.99e-30 124
Msa0835240 AT1G07520.3 25.260 384 272 8 120 495 319 695 1.96e-29 122
Msa0835240 AT1G07520.1 25.260 384 272 8 120 495 318 694 2.07e-29 122
Msa0835240 AT2G29060.2 24.869 382 272 8 120 495 284 656 3.08e-29 122
Msa0835240 AT2G29060.1 24.869 382 272 8 120 495 321 693 3.15e-29 122
Msa0835240 AT3G50650.1 28.608 388 247 11 118 494 174 542 3.76e-29 120
Msa0835240 AT1G07520.2 25.260 384 272 8 120 495 154 530 4.00e-29 120
Msa0835240 AT1G07520.4 25.260 384 272 8 120 495 154 530 4.00e-29 120
Msa0835240 AT4G08250.1 27.047 403 261 16 104 493 94 476 5.62e-24 105
Msa0835240 AT4G00150.1 23.664 393 233 14 122 494 209 554 3.39e-13 72.4
Msa0835240 AT3G60630.1 24.919 309 199 12 200 494 333 622 6.78e-12 68.2

Find 120 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGGCTGATTTAAATTT+TGG 0.142361 5_4:+8557836 Msa0835240:CDS
TGAATAACGTTGAATTTAAA+TGG 0.226774 5_4:-8557626 None:intergenic
CTTCTTGTGGATTGTAATAT+TGG 0.229803 5_4:-8556899 None:intergenic
GATTTCACCTTTGAATTAAC+TGG 0.256797 5_4:+8557074 Msa0835240:CDS
AAGAGTGTTTGAGATGGTTT+AGG 0.270052 5_4:+8557891 Msa0835240:CDS
GGCTTTGTTGAGGAGGTATA+AGG 0.272310 5_4:+8558129 Msa0835240:CDS
TTCGGTCATTCGAGAGAATA+AGG 0.284815 5_4:-8557246 None:intergenic
CTCTCCCAGCTTCCCTTTCA+AGG 0.286911 5_4:-8557970 None:intergenic
ACCTTCGAAGGCTTCTAAAA+TGG 0.301815 5_4:-8557405 None:intergenic
GGGGTGTGAGTGATGGTGTG+GGG 0.332972 5_4:-8557037 None:intergenic
GATCTTCTTGTTGTTGTTGT+TGG 0.340983 5_4:-8556806 None:intergenic
TGGGAGAGGGATAGTTGATT+TGG 0.342461 5_4:+8557985 Msa0835240:CDS
GGGTGTGAGTGATGGTGTGG+GGG 0.342781 5_4:-8557036 None:intergenic
AAATCAGTTGTTGGTGGAGG+GGG 0.353733 5_4:-8557056 None:intergenic
GGTGGTGGTGTTGGTGTGGT+TGG 0.357217 5_4:-8556785 None:intergenic
AGGAAGTTTGTGATGATGTT+AGG 0.369294 5_4:+8558107 Msa0835240:CDS
GAAGGTTCTGGTACCGGCTT+CGG 0.378697 5_4:-8557264 None:intergenic
AGAGTGTTTGAGATGGTTTA+GGG 0.380120 5_4:+8557892 Msa0835240:CDS
GAGGTTTGAGGTTGACTATA+AGG 0.384294 5_4:-8556993 None:intergenic
TAGTCCATGGACTAACTTCT+TGG 0.385254 5_4:-8557355 None:intergenic
CGTTGATCGCGTTGCGGCCG+AGG 0.391480 5_4:+8557795 Msa0835240:CDS
ACTTGATGTTGTTGTTCTGC+TGG 0.396312 5_4:-8556847 None:intergenic
CATTCTTCTTCAACTTCTTG+TGG 0.400632 5_4:-8556912 None:intergenic
CTTCCACGTCAAGAATATTC+CGG 0.409628 5_4:-8558182 None:intergenic
GTTGTTGTTGGTGGTGGTGT+TGG 0.411979 5_4:-8556794 None:intergenic
GTTAGTCCATGGACTACTTT+CGG 0.413327 5_4:+8557362 Msa0835240:CDS
AGCTAGGGTTCTGAAGGTTC+TGG 0.415747 5_4:-8557276 None:intergenic
GTTTGTAGTAATGGTGTTTG+AGG 0.420379 5_4:-8557012 None:intergenic
ACTATAAGGATAGTAGTGTT+TGG 0.430455 5_4:-8556979 None:intergenic
GAAGGAAATTGGTGCTAGAA+TGG 0.434419 5_4:+8557577 Msa0835240:CDS
TTGATCCTTGAAAGGGAAGC+TGG 0.440745 5_4:+8557965 Msa0835240:CDS
AACGAACTAAGCTCCCCTTA+TGG 0.445036 5_4:+8557185 Msa0835240:CDS
GTAATGGTGTTTGAGGTTTG+AGG 0.447709 5_4:-8557005 None:intergenic
TTCTCTCGAATGACCGAAGC+CGG 0.449633 5_4:+8557251 Msa0835240:CDS
TCACGGCGTTTGCATGGTGG+AGG 0.454306 5_4:+8558061 Msa0835240:CDS
CAGTGACGATGGTGGTTAAA+CGG 0.455896 5_4:-8557529 None:intergenic
ATGTTAGGGCTTTGTTGAGG+AGG 0.458311 5_4:+8558122 Msa0835240:CDS
TTCGGCCATGTTGCATGCAA+TGG 0.459991 5_4:+8557380 Msa0835240:CDS
TTTGTTCTATGTCACCATAA+GGG 0.462505 5_4:-8557199 None:intergenic
CTATAGCTCGTGCTGTTTCG+AGG 0.464786 5_4:-8557118 None:intergenic
CGGTGGTGTGGGCCAGTGCA+TGG 0.470323 5_4:+8558212 Msa0835240:CDS
TGAAATCAGTTGTTGGTGGA+GGG 0.471301 5_4:-8557058 None:intergenic
CATGCACTGGCCCACACCAC+CGG 0.476107 5_4:-8558211 None:intergenic
GTGAAATCAGTTGTTGGTGG+AGG 0.476494 5_4:-8557059 None:intergenic
GGAAGTTTGTGATGATGTTA+GGG 0.478484 5_4:+8558108 Msa0835240:CDS
CACGGCGTTTGCATGGTGGA+GGG 0.480736 5_4:+8558062 Msa0835240:CDS
GGTTCTGAAGGTTCTGGTAC+CGG 0.480940 5_4:-8557270 None:intergenic
AAACGCTCGTTACTCGTCTT+CGG 0.484291 5_4:-8557944 None:intergenic
TGTTGTTGTTCTGCTGGTGG+TGG 0.490954 5_4:-8556841 None:intergenic
ATGGTTTAGGGTTTACTTTG+AGG 0.492134 5_4:+8557904 Msa0835240:CDS
GATGGATATGAATTTGTGAA+TGG 0.492688 5_4:+8557863 Msa0835240:CDS
TCAAAGGTGAAATCAGTTGT+TGG 0.501730 5_4:-8557065 None:intergenic
TGTTTCGAGGAGGATGTTGT+TGG 0.505657 5_4:-8557105 None:intergenic
CCACTTTCCAGTTAATTCAA+AGG 0.510883 5_4:-8557081 None:intergenic
GAAATCAGTTGTTGGTGGAG+GGG 0.511614 5_4:-8557057 None:intergenic
GCATGGTGGAGGGTTTAATA+CGG 0.512491 5_4:+8558072 Msa0835240:CDS
CCTTTGAATTAACTGGAAAG+TGG 0.516151 5_4:+8557081 Msa0835240:CDS
TGATTTGGTGGCATGTGCTC+CGG 0.516469 5_4:+8558000 Msa0835240:CDS
TTATCAAGGCCTCCATGCAC+TGG 0.517596 5_4:-8558224 None:intergenic
GGAAGGAACAACCGGTGGTG+TGG 0.520705 5_4:+8558200 Msa0835240:CDS
GAGAGGGATAGTTGATTTGG+TGG 0.522738 5_4:+8557988 Msa0835240:CDS
CTTGACGTGGAAGGAACAAC+CGG 0.523785 5_4:+8558192 Msa0835240:CDS
AGGAAGGGTGGTCGATGATA+CGG 0.526878 5_4:+8558149 Msa0835240:CDS
CTCCGTTCAATCGACTCTGC+CGG 0.527978 5_4:-8558019 None:intergenic
ATTCAACGTTATTCATCACT+CGG 0.529452 5_4:+8557634 Msa0835240:CDS
CGAGCGTTTGATCCTTGAAA+GGG 0.531173 5_4:+8557958 Msa0835240:CDS
GTAACAGTTGGTAATGATAA+CGG 0.532840 5_4:+8557743 Msa0835240:CDS
GAAGGAACAACCGGTGGTGT+GGG 0.535300 5_4:+8558201 Msa0835240:CDS
GTCGATTGAACGGAGAGAAA+CGG 0.535584 5_4:+8558027 Msa0835240:CDS
TCAACCACTGTTACTATCCT+CGG 0.540676 5_4:-8557812 None:intergenic
TTGTTCTATGTCACCATAAG+GGG 0.542773 5_4:-8557198 None:intergenic
CGGAATATTCTTGACGTGGA+AGG 0.543303 5_4:+8558183 Msa0835240:CDS
TGATGTTGTTGTTCTGCTGG+TGG 0.544428 5_4:-8556844 None:intergenic
CTTGAAAGGGAAGCTGGGAG+AGG 0.545509 5_4:+8557971 Msa0835240:CDS
TGTTGGTGGTGGTGTTGGTG+TGG 0.547970 5_4:-8556789 None:intergenic
GGAGAGAAACGGCGGTGAGA+TGG 0.549109 5_4:+8558038 Msa0835240:CDS
TTAACCACCATCGTCACTGC+TGG 0.551969 5_4:+8557533 Msa0835240:CDS
GATAGTAACAGTGGTTGAAG+AGG 0.553960 5_4:+8557817 Msa0835240:CDS
TGCTGAAGCTAGGGTTCTGA+AGG 0.554762 5_4:-8557282 None:intergenic
GCTTTGCATTCAGTAACAGT+TGG 0.557029 5_4:+8557731 Msa0835240:CDS
AGATGGTCACGGCGTTTGCA+TGG 0.559260 5_4:+8558055 Msa0835240:CDS
CTTTGAATTAACTGGAAAGT+GGG 0.560664 5_4:+8557082 Msa0835240:CDS
ACGAGCGTTTGATCCTTGAA+AGG 0.564670 5_4:+8557957 Msa0835240:CDS
AATTCCAAGAAGTTAGTCCA+TGG 0.567956 5_4:+8557351 Msa0835240:CDS
CAACTTAGAATCACCTTCGA+AGG 0.574160 5_4:-8557417 None:intergenic
ATGATACGGTGTAATGATGC+CGG 0.576413 5_4:+8558163 Msa0835240:CDS
ACCACCATCGTCACTGCTGG+TGG 0.581472 5_4:+8557536 Msa0835240:CDS
AGTAACAGTGGTTGAAGAGG+AGG 0.584206 5_4:+8557820 Msa0835240:CDS
CTCCGGCAGAGTCGATTGAA+CGG 0.585476 5_4:+8558017 Msa0835240:CDS
TGATCCTTGAAAGGGAAGCT+GGG 0.585745 5_4:+8557966 Msa0835240:CDS
TGTTGAGGAGGTATAAGGAA+GGG 0.588341 5_4:+8558134 Msa0835240:CDS
TTGAAAGGGAAGCTGGGAGA+GGG 0.589847 5_4:+8557972 Msa0835240:CDS
TTGTTGAGGAGGTATAAGGA+AGG 0.589974 5_4:+8558133 Msa0835240:CDS
CGGAGTATCATCAGCACGAG+TGG 0.595472 5_4:-8557501 None:intergenic
AAGGTGAAATCAGTTGTTGG+TGG 0.600375 5_4:-8557062 None:intergenic
TCATTCGAGAGAATAAGGCT+TGG 0.601622 5_4:-8557241 None:intergenic
TTCAACGTTATTCATCACTC+GGG 0.607328 5_4:+8557635 Msa0835240:CDS
ATGATGTTAGGGCTTTGTTG+AGG 0.607674 5_4:+8558119 Msa0835240:CDS
CTCGTTTACAACAACTCATG+TGG 0.608017 5_4:+8557156 Msa0835240:CDS
ACATGGCCGAAAGTAGTCCA+TGG 0.613056 5_4:-8557368 None:intergenic
GTTTGGAAGAAGATGATGAA+AGG 0.615269 5_4:-8556962 None:intergenic
AAGCTATCAACGCGTCGCGA+CGG 0.617261 5_4:-8557772 None:intergenic
TGGCGCCATTGCATGCAACA+TGG 0.621243 5_4:-8557385 None:intergenic
TGAGGAGGTATAAGGAAGGG+TGG 0.622191 5_4:+8558137 Msa0835240:CDS
TAGCTTCGTTGATCGCGTTG+CGG 0.632402 5_4:+8557789 Msa0835240:CDS
TGGTCACGGCGTTTGCATGG+TGG 0.640251 5_4:+8558058 Msa0835240:CDS
AAACGGCGGTGAGATGGTCA+CGG 0.645102 5_4:+8558044 Msa0835240:CDS
TAATACGGTGGCGTTTAGTG+AGG 0.648084 5_4:+8558087 Msa0835240:CDS
AGAACCACCAGCAGTGACGA+TGG 0.652328 5_4:-8557540 None:intergenic
TGGTGGAGGGTTTAATACGG+TGG 0.662791 5_4:+8558075 Msa0835240:CDS
ATGCCGGAATATTCTTGACG+TGG 0.670161 5_4:+8558179 Msa0835240:CDS
GTAACACTTATTCCACTCAG+TGG 0.674128 5_4:+8557456 Msa0835240:CDS
ATGGTGGTTAAACGGAGGTG+CGG 0.674756 5_4:-8557521 None:intergenic
TAGCTCGTGCTGTTTCGAGG+AGG 0.677191 5_4:-8557115 None:intergenic
TGGTGTGGGCCAGTGCATGG+AGG 0.695562 5_4:+8558215 Msa0835240:CDS
GACGTGGAAGGAACAACCGG+TGG 0.699506 5_4:+8558195 Msa0835240:CDS
GCGGCCGAGGATAGTAACAG+TGG 0.704672 5_4:+8557808 Msa0835240:CDS
ACCACCAGCAGTGACGATGG+TGG 0.722652 5_4:-8557537 None:intergenic
GATTGAACGGAGAGAAACGG+CGG 0.736491 5_4:+8558030 Msa0835240:CDS
TGACGATGGTGGTTAAACGG+AGG 0.736521 5_4:-8557526 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTCTGATTTAAATTTTT+TGG + chr5_4:8557661-8557680 Msa0835240:CDS 15.0%
!!! TCTTTCTGATTTAAATTTTT+TGG + chr5_4:8557661-8557680 Msa0835240:CDS 15.0%
!! TGAATAACGTTGAATTTAAA+TGG - chr5_4:8557629-8557648 None:intergenic 20.0%
!!! GATTTAAATTTTGGTAATGA+TGG + chr5_4:8557845-8557864 Msa0835240:CDS 20.0%
!! TGAATAACGTTGAATTTAAA+TGG - chr5_4:8557629-8557648 None:intergenic 20.0%
!!! GATTTAAATTTTGGTAATGA+TGG + chr5_4:8557845-8557864 Msa0835240:CDS 20.0%
! TTCTGTTCAAAAAGTTATGA+AGG + chr5_4:8557559-8557578 Msa0835240:CDS 25.0%
! CAAAAAGTTATGAAGGAAAT+TGG + chr5_4:8557566-8557585 Msa0835240:CDS 25.0%
! TTCTGTTCAAAAAGTTATGA+AGG + chr5_4:8557559-8557578 Msa0835240:CDS 25.0%
! CAAAAAGTTATGAAGGAAAT+TGG + chr5_4:8557566-8557585 Msa0835240:CDS 25.0%
!!! TTGGTGATAGTTTTGTTTTG+AGG - chr5_4:8556883-8556902 None:intergenic 30.0%
! CTTCTTGTGGATTGTAATAT+TGG - chr5_4:8556902-8556921 None:intergenic 30.0%
!!! AGAATGCTTCAACTTTTTCA+TGG + chr5_4:8556929-8556948 Msa0835240:CDS 30.0%
ACTATAAGGATAGTAGTGTT+TGG - chr5_4:8556982-8557001 None:intergenic 30.0%
GATTTCACCTTTGAATTAAC+TGG + chr5_4:8557074-8557093 Msa0835240:CDS 30.0%
CTTTGAATTAACTGGAAAGT+GGG + chr5_4:8557082-8557101 Msa0835240:CDS 30.0%
TTTGTTCTATGTCACCATAA+GGG - chr5_4:8557202-8557221 None:intergenic 30.0%
! TTTTGTTCTATGTCACCATA+AGG - chr5_4:8557203-8557222 None:intergenic 30.0%
! GACTAACTTCTTGGAATTTT+AGG - chr5_4:8557349-8557368 None:intergenic 30.0%
GAAAAATTCGCTAGACTTAT+GGG + chr5_4:8557599-8557618 Msa0835240:CDS 30.0%
ATTCAACGTTATTCATCACT+CGG + chr5_4:8557634-8557653 Msa0835240:CDS 30.0%
!! GTAACAGTTGGTAATGATAA+CGG + chr5_4:8557743-8557762 Msa0835240:CDS 30.0%
GATGGATATGAATTTGTGAA+TGG + chr5_4:8557863-8557882 Msa0835240:CDS 30.0%
!!! TTGGTGATAGTTTTGTTTTG+AGG - chr5_4:8556883-8556902 None:intergenic 30.0%
! CTTCTTGTGGATTGTAATAT+TGG - chr5_4:8556902-8556921 None:intergenic 30.0%
!!! AGAATGCTTCAACTTTTTCA+TGG + chr5_4:8556929-8556948 Msa0835240:CDS 30.0%
ACTATAAGGATAGTAGTGTT+TGG - chr5_4:8556982-8557001 None:intergenic 30.0%
GATTTCACCTTTGAATTAAC+TGG + chr5_4:8557074-8557093 Msa0835240:CDS 30.0%
CTTTGAATTAACTGGAAAGT+GGG + chr5_4:8557082-8557101 Msa0835240:CDS 30.0%
TTTGTTCTATGTCACCATAA+GGG - chr5_4:8557202-8557221 None:intergenic 30.0%
! TTTTGTTCTATGTCACCATA+AGG - chr5_4:8557203-8557222 None:intergenic 30.0%
! GACTAACTTCTTGGAATTTT+AGG - chr5_4:8557349-8557368 None:intergenic 30.0%
GAAAAATTCGCTAGACTTAT+GGG + chr5_4:8557599-8557618 Msa0835240:CDS 30.0%
ATTCAACGTTATTCATCACT+CGG + chr5_4:8557634-8557653 Msa0835240:CDS 30.0%
!! GTAACAGTTGGTAATGATAA+CGG + chr5_4:8557743-8557762 Msa0835240:CDS 30.0%
GATGGATATGAATTTGTGAA+TGG + chr5_4:8557863-8557882 Msa0835240:CDS 30.0%
!! GATCTTCTTGTTGTTGTTGT+TGG - chr5_4:8556809-8556828 None:intergenic 35.0%
CATTCTTCTTCAACTTCTTG+TGG - chr5_4:8556915-8556934 None:intergenic 35.0%
GTTTGGAAGAAGATGATGAA+AGG - chr5_4:8556965-8556984 None:intergenic 35.0%
GTTTGTAGTAATGGTGTTTG+AGG - chr5_4:8557015-8557034 None:intergenic 35.0%
TCAAAGGTGAAATCAGTTGT+TGG - chr5_4:8557068-8557087 None:intergenic 35.0%
CCACTTTCCAGTTAATTCAA+AGG - chr5_4:8557084-8557103 None:intergenic 35.0%
CCTTTGAATTAACTGGAAAG+TGG + chr5_4:8557081-8557100 Msa0835240:CDS 35.0%
TTGTTCTATGTCACCATAAG+GGG - chr5_4:8557201-8557220 None:intergenic 35.0%
! TTTTTCTGATGCTGAAGCTA+GGG - chr5_4:8557294-8557313 None:intergenic 35.0%
!!! TTTTTTCTGATGCTGAAGCT+AGG - chr5_4:8557295-8557314 None:intergenic 35.0%
AATTCCAAGAAGTTAGTCCA+TGG + chr5_4:8557351-8557370 Msa0835240:CDS 35.0%
GGAAAAATTCGCTAGACTTA+TGG + chr5_4:8557598-8557617 Msa0835240:CDS 35.0%
TTCAACGTTATTCATCACTC+GGG + chr5_4:8557635-8557654 Msa0835240:CDS 35.0%
!! GAGGAGGCTGATTTAAATTT+TGG + chr5_4:8557836-8557855 Msa0835240:CDS 35.0%
!!! GTTTTGAAGAGTGTTTGAGA+TGG + chr5_4:8557885-8557904 Msa0835240:CDS 35.0%
!! AAGAGTGTTTGAGATGGTTT+AGG + chr5_4:8557891-8557910 Msa0835240:CDS 35.0%
! AGAGTGTTTGAGATGGTTTA+GGG + chr5_4:8557892-8557911 Msa0835240:CDS 35.0%
! ATGGTTTAGGGTTTACTTTG+AGG + chr5_4:8557904-8557923 Msa0835240:CDS 35.0%
! AGGAAGTTTGTGATGATGTT+AGG + chr5_4:8558107-8558126 Msa0835240:CDS 35.0%
! GGAAGTTTGTGATGATGTTA+GGG + chr5_4:8558108-8558127 Msa0835240:CDS 35.0%
!! GATCTTCTTGTTGTTGTTGT+TGG - chr5_4:8556809-8556828 None:intergenic 35.0%
CATTCTTCTTCAACTTCTTG+TGG - chr5_4:8556915-8556934 None:intergenic 35.0%
GTTTGGAAGAAGATGATGAA+AGG - chr5_4:8556965-8556984 None:intergenic 35.0%
GTTTGTAGTAATGGTGTTTG+AGG - chr5_4:8557015-8557034 None:intergenic 35.0%
TCAAAGGTGAAATCAGTTGT+TGG - chr5_4:8557068-8557087 None:intergenic 35.0%
CCACTTTCCAGTTAATTCAA+AGG - chr5_4:8557084-8557103 None:intergenic 35.0%
CCTTTGAATTAACTGGAAAG+TGG + chr5_4:8557081-8557100 Msa0835240:CDS 35.0%
TTGTTCTATGTCACCATAAG+GGG - chr5_4:8557201-8557220 None:intergenic 35.0%
! TTTTTCTGATGCTGAAGCTA+GGG - chr5_4:8557294-8557313 None:intergenic 35.0%
!!! TTTTTTCTGATGCTGAAGCT+AGG - chr5_4:8557295-8557314 None:intergenic 35.0%
AATTCCAAGAAGTTAGTCCA+TGG + chr5_4:8557351-8557370 Msa0835240:CDS 35.0%
GGAAAAATTCGCTAGACTTA+TGG + chr5_4:8557598-8557617 Msa0835240:CDS 35.0%
TTCAACGTTATTCATCACTC+GGG + chr5_4:8557635-8557654 Msa0835240:CDS 35.0%
!! GAGGAGGCTGATTTAAATTT+TGG + chr5_4:8557836-8557855 Msa0835240:CDS 35.0%
!!! GTTTTGAAGAGTGTTTGAGA+TGG + chr5_4:8557885-8557904 Msa0835240:CDS 35.0%
!! AAGAGTGTTTGAGATGGTTT+AGG + chr5_4:8557891-8557910 Msa0835240:CDS 35.0%
! AGAGTGTTTGAGATGGTTTA+GGG + chr5_4:8557892-8557911 Msa0835240:CDS 35.0%
! ATGGTTTAGGGTTTACTTTG+AGG + chr5_4:8557904-8557923 Msa0835240:CDS 35.0%
! AGGAAGTTTGTGATGATGTT+AGG + chr5_4:8558107-8558126 Msa0835240:CDS 35.0%
! GGAAGTTTGTGATGATGTTA+GGG + chr5_4:8558108-8558127 Msa0835240:CDS 35.0%
!! CTTCTTGTTGTTGTTGTTGG+TGG - chr5_4:8556806-8556825 None:intergenic 40.0%
! ACTTGATGTTGTTGTTCTGC+TGG - chr5_4:8556850-8556869 None:intergenic 40.0%
! GAGGTTTGAGGTTGACTATA+AGG - chr5_4:8556996-8557015 None:intergenic 40.0%
! GTAATGGTGTTTGAGGTTTG+AGG - chr5_4:8557008-8557027 None:intergenic 40.0%
! TGAAATCAGTTGTTGGTGGA+GGG - chr5_4:8557061-8557080 None:intergenic 40.0%
AAGGTGAAATCAGTTGTTGG+TGG - chr5_4:8557065-8557084 None:intergenic 40.0%
CTCGTTTACAACAACTCATG+TGG + chr5_4:8557156-8557175 Msa0835240:CDS 40.0%
TCATTCGAGAGAATAAGGCT+TGG - chr5_4:8557244-8557263 None:intergenic 40.0%
TTCGGTCATTCGAGAGAATA+AGG - chr5_4:8557249-8557268 None:intergenic 40.0%
! TAGTCCATGGACTAACTTCT+TGG - chr5_4:8557358-8557377 None:intergenic 40.0%
! GTTAGTCCATGGACTACTTT+CGG + chr5_4:8557362-8557381 Msa0835240:CDS 40.0%
! ACCTTCGAAGGCTTCTAAAA+TGG - chr5_4:8557408-8557427 None:intergenic 40.0%
CAACTTAGAATCACCTTCGA+AGG - chr5_4:8557420-8557439 None:intergenic 40.0%
GTAACACTTATTCCACTCAG+TGG + chr5_4:8557456-8557475 Msa0835240:CDS 40.0%
!! GAAGGAAATTGGTGCTAGAA+TGG + chr5_4:8557577-8557596 Msa0835240:CDS 40.0%
GCTTTGCATTCAGTAACAGT+TGG + chr5_4:8557731-8557750 Msa0835240:CDS 40.0%
TCAACCACTGTTACTATCCT+CGG - chr5_4:8557815-8557834 None:intergenic 40.0%
GATAGTAACAGTGGTTGAAG+AGG + chr5_4:8557817-8557836 Msa0835240:CDS 40.0%
!! ATGATGTTAGGGCTTTGTTG+AGG + chr5_4:8558119-8558138 Msa0835240:CDS 40.0%
! TTGTTGAGGAGGTATAAGGA+AGG + chr5_4:8558133-8558152 Msa0835240:CDS 40.0%
! TGTTGAGGAGGTATAAGGAA+GGG + chr5_4:8558134-8558153 Msa0835240:CDS 40.0%
ATGATACGGTGTAATGATGC+CGG + chr5_4:8558163-8558182 Msa0835240:CDS 40.0%
CTTCCACGTCAAGAATATTC+CGG - chr5_4:8558185-8558204 None:intergenic 40.0%
!! CTTCTTGTTGTTGTTGTTGG+TGG - chr5_4:8556806-8556825 None:intergenic 40.0%
! ACTTGATGTTGTTGTTCTGC+TGG - chr5_4:8556850-8556869 None:intergenic 40.0%
! GAGGTTTGAGGTTGACTATA+AGG - chr5_4:8556996-8557015 None:intergenic 40.0%
! GTAATGGTGTTTGAGGTTTG+AGG - chr5_4:8557008-8557027 None:intergenic 40.0%
! TGAAATCAGTTGTTGGTGGA+GGG - chr5_4:8557061-8557080 None:intergenic 40.0%
AAGGTGAAATCAGTTGTTGG+TGG - chr5_4:8557065-8557084 None:intergenic 40.0%
CTCGTTTACAACAACTCATG+TGG + chr5_4:8557156-8557175 Msa0835240:CDS 40.0%
TCATTCGAGAGAATAAGGCT+TGG - chr5_4:8557244-8557263 None:intergenic 40.0%
TTCGGTCATTCGAGAGAATA+AGG - chr5_4:8557249-8557268 None:intergenic 40.0%
! TAGTCCATGGACTAACTTCT+TGG - chr5_4:8557358-8557377 None:intergenic 40.0%
! GTTAGTCCATGGACTACTTT+CGG + chr5_4:8557362-8557381 Msa0835240:CDS 40.0%
! ACCTTCGAAGGCTTCTAAAA+TGG - chr5_4:8557408-8557427 None:intergenic 40.0%
CAACTTAGAATCACCTTCGA+AGG - chr5_4:8557420-8557439 None:intergenic 40.0%
GTAACACTTATTCCACTCAG+TGG + chr5_4:8557456-8557475 Msa0835240:CDS 40.0%
!! GAAGGAAATTGGTGCTAGAA+TGG + chr5_4:8557577-8557596 Msa0835240:CDS 40.0%
GCTTTGCATTCAGTAACAGT+TGG + chr5_4:8557731-8557750 Msa0835240:CDS 40.0%
TCAACCACTGTTACTATCCT+CGG - chr5_4:8557815-8557834 None:intergenic 40.0%
GATAGTAACAGTGGTTGAAG+AGG + chr5_4:8557817-8557836 Msa0835240:CDS 40.0%
!! ATGATGTTAGGGCTTTGTTG+AGG + chr5_4:8558119-8558138 Msa0835240:CDS 40.0%
! TTGTTGAGGAGGTATAAGGA+AGG + chr5_4:8558133-8558152 Msa0835240:CDS 40.0%
! TGTTGAGGAGGTATAAGGAA+GGG + chr5_4:8558134-8558153 Msa0835240:CDS 40.0%
ATGATACGGTGTAATGATGC+CGG + chr5_4:8558163-8558182 Msa0835240:CDS 40.0%
CTTCCACGTCAAGAATATTC+CGG - chr5_4:8558185-8558204 None:intergenic 40.0%
!! CTTGTTGTTGTTGTTGGTGG+TGG - chr5_4:8556803-8556822 None:intergenic 45.0%
! TGATGTTGTTGTTCTGCTGG+TGG - chr5_4:8556847-8556866 None:intergenic 45.0%
!! TGTGGGGGTGTTTGTAGTAA+TGG - chr5_4:8557024-8557043 None:intergenic 45.0%
! AAATCAGTTGTTGGTGGAGG+GGG - chr5_4:8557059-8557078 None:intergenic 45.0%
! GAAATCAGTTGTTGGTGGAG+GGG - chr5_4:8557060-8557079 None:intergenic 45.0%
! GTGAAATCAGTTGTTGGTGG+AGG - chr5_4:8557062-8557081 None:intergenic 45.0%
TGTTTCGAGGAGGATGTTGT+TGG - chr5_4:8557108-8557127 None:intergenic 45.0%
AACGAACTAAGCTCCCCTTA+TGG + chr5_4:8557185-8557204 Msa0835240:CDS 45.0%
!! GCCATTTTAGAAGCCTTCGA+AGG + chr5_4:8557404-8557423 Msa0835240:CDS 45.0%
! CAGTGACGATGGTGGTTAAA+CGG - chr5_4:8557532-8557551 None:intergenic 45.0%
AGTAACAGTGGTTGAAGAGG+AGG + chr5_4:8557820-8557839 Msa0835240:CDS 45.0%
AAACGCTCGTTACTCGTCTT+CGG - chr5_4:8557947-8557966 None:intergenic 45.0%
ACGAGCGTTTGATCCTTGAA+AGG + chr5_4:8557957-8557976 Msa0835240:CDS 45.0%
CGAGCGTTTGATCCTTGAAA+GGG + chr5_4:8557958-8557977 Msa0835240:CDS 45.0%
TTGATCCTTGAAAGGGAAGC+TGG + chr5_4:8557965-8557984 Msa0835240:CDS 45.0%
TGATCCTTGAAAGGGAAGCT+GGG + chr5_4:8557966-8557985 Msa0835240:CDS 45.0%
TGGGAGAGGGATAGTTGATT+TGG + chr5_4:8557985-8558004 Msa0835240:CDS 45.0%
!! GAGAGGGATAGTTGATTTGG+TGG + chr5_4:8557988-8558007 Msa0835240:CDS 45.0%
GTCGATTGAACGGAGAGAAA+CGG + chr5_4:8558027-8558046 Msa0835240:CDS 45.0%
! GCATGGTGGAGGGTTTAATA+CGG + chr5_4:8558072-8558091 Msa0835240:CDS 45.0%
TAATACGGTGGCGTTTAGTG+AGG + chr5_4:8558087-8558106 Msa0835240:CDS 45.0%
!! ATGTTAGGGCTTTGTTGAGG+AGG + chr5_4:8558122-8558141 Msa0835240:CDS 45.0%
!! GGCTTTGTTGAGGAGGTATA+AGG + chr5_4:8558129-8558148 Msa0835240:CDS 45.0%
ATGCCGGAATATTCTTGACG+TGG + chr5_4:8558179-8558198 Msa0835240:CDS 45.0%
CGGAATATTCTTGACGTGGA+AGG + chr5_4:8558183-8558202 Msa0835240:CDS 45.0%
!! CTTGTTGTTGTTGTTGGTGG+TGG - chr5_4:8556803-8556822 None:intergenic 45.0%
! TGATGTTGTTGTTCTGCTGG+TGG - chr5_4:8556847-8556866 None:intergenic 45.0%
!! TGTGGGGGTGTTTGTAGTAA+TGG - chr5_4:8557024-8557043 None:intergenic 45.0%
! AAATCAGTTGTTGGTGGAGG+GGG - chr5_4:8557059-8557078 None:intergenic 45.0%
! GAAATCAGTTGTTGGTGGAG+GGG - chr5_4:8557060-8557079 None:intergenic 45.0%
! GTGAAATCAGTTGTTGGTGG+AGG - chr5_4:8557062-8557081 None:intergenic 45.0%
TGTTTCGAGGAGGATGTTGT+TGG - chr5_4:8557108-8557127 None:intergenic 45.0%
AACGAACTAAGCTCCCCTTA+TGG + chr5_4:8557185-8557204 Msa0835240:CDS 45.0%
!! GCCATTTTAGAAGCCTTCGA+AGG + chr5_4:8557404-8557423 Msa0835240:CDS 45.0%
! CAGTGACGATGGTGGTTAAA+CGG - chr5_4:8557532-8557551 None:intergenic 45.0%
AGTAACAGTGGTTGAAGAGG+AGG + chr5_4:8557820-8557839 Msa0835240:CDS 45.0%
AAACGCTCGTTACTCGTCTT+CGG - chr5_4:8557947-8557966 None:intergenic 45.0%
ACGAGCGTTTGATCCTTGAA+AGG + chr5_4:8557957-8557976 Msa0835240:CDS 45.0%
CGAGCGTTTGATCCTTGAAA+GGG + chr5_4:8557958-8557977 Msa0835240:CDS 45.0%
TTGATCCTTGAAAGGGAAGC+TGG + chr5_4:8557965-8557984 Msa0835240:CDS 45.0%
TGATCCTTGAAAGGGAAGCT+GGG + chr5_4:8557966-8557985 Msa0835240:CDS 45.0%
TGGGAGAGGGATAGTTGATT+TGG + chr5_4:8557985-8558004 Msa0835240:CDS 45.0%
!! GAGAGGGATAGTTGATTTGG+TGG + chr5_4:8557988-8558007 Msa0835240:CDS 45.0%
GTCGATTGAACGGAGAGAAA+CGG + chr5_4:8558027-8558046 Msa0835240:CDS 45.0%
! GCATGGTGGAGGGTTTAATA+CGG + chr5_4:8558072-8558091 Msa0835240:CDS 45.0%
TAATACGGTGGCGTTTAGTG+AGG + chr5_4:8558087-8558106 Msa0835240:CDS 45.0%
!! ATGTTAGGGCTTTGTTGAGG+AGG + chr5_4:8558122-8558141 Msa0835240:CDS 45.0%
!! GGCTTTGTTGAGGAGGTATA+AGG + chr5_4:8558129-8558148 Msa0835240:CDS 45.0%
ATGCCGGAATATTCTTGACG+TGG + chr5_4:8558179-8558198 Msa0835240:CDS 45.0%
CGGAATATTCTTGACGTGGA+AGG + chr5_4:8558183-8558202 Msa0835240:CDS 45.0%
!! GTTGTTGTTGGTGGTGGTGT+TGG - chr5_4:8556797-8556816 None:intergenic 50.0%
! TGTTGTTGTTCTGCTGGTGG+TGG - chr5_4:8556844-8556863 None:intergenic 50.0%
!! CTATAGCTCGTGCTGTTTCG+AGG - chr5_4:8557121-8557140 None:intergenic 50.0%
TTCTCTCGAATGACCGAAGC+CGG + chr5_4:8557251-8557270 Msa0835240:CDS 50.0%
!! GGTTCTGAAGGTTCTGGTAC+CGG - chr5_4:8557273-8557292 None:intergenic 50.0%
!! AGCTAGGGTTCTGAAGGTTC+TGG - chr5_4:8557279-8557298 None:intergenic 50.0%
! TGCTGAAGCTAGGGTTCTGA+AGG - chr5_4:8557285-8557304 None:intergenic 50.0%
ACATGGCCGAAAGTAGTCCA+TGG - chr5_4:8557371-8557390 None:intergenic 50.0%
TTCGGCCATGTTGCATGCAA+TGG + chr5_4:8557380-8557399 Msa0835240:CDS 50.0%
!! AAAAAGCGTTGGCCACTGAG+TGG - chr5_4:8557471-8557490 None:intergenic 50.0%
!!! GCCAACGCTTTTTGAAGCGT+TGG + chr5_4:8557478-8557497 Msa0835240:CDS 50.0%
! GCCAACGCTTCAAAAAGCGT+TGG - chr5_4:8557482-8557501 None:intergenic 50.0%
ATGGTGGTTAAACGGAGGTG+CGG - chr5_4:8557524-8557543 None:intergenic 50.0%
! TGACGATGGTGGTTAAACGG+AGG - chr5_4:8557529-8557548 None:intergenic 50.0%
TTAACCACCATCGTCACTGC+TGG + chr5_4:8557533-8557552 Msa0835240:CDS 50.0%
TAGCTTCGTTGATCGCGTTG+CGG + chr5_4:8557789-8557808 Msa0835240:CDS 50.0%
TTGAAAGGGAAGCTGGGAGA+GGG + chr5_4:8557972-8557991 Msa0835240:CDS 50.0%
!! TGATTTGGTGGCATGTGCTC+CGG + chr5_4:8558000-8558019 Msa0835240:CDS 50.0%
GATTGAACGGAGAGAAACGG+CGG + chr5_4:8558030-8558049 Msa0835240:CDS 50.0%
TGGTGGAGGGTTTAATACGG+TGG + chr5_4:8558075-8558094 Msa0835240:CDS 50.0%
! TGAGGAGGTATAAGGAAGGG+TGG + chr5_4:8558137-8558156 Msa0835240:CDS 50.0%
AGGAAGGGTGGTCGATGATA+CGG + chr5_4:8558149-8558168 Msa0835240:CDS 50.0%
CTTGACGTGGAAGGAACAAC+CGG + chr5_4:8558192-8558211 Msa0835240:CDS 50.0%
!! GTTGTTGTTGGTGGTGGTGT+TGG - chr5_4:8556797-8556816 None:intergenic 50.0%
! TGTTGTTGTTCTGCTGGTGG+TGG - chr5_4:8556844-8556863 None:intergenic 50.0%
!! CTATAGCTCGTGCTGTTTCG+AGG - chr5_4:8557121-8557140 None:intergenic 50.0%
TTCTCTCGAATGACCGAAGC+CGG + chr5_4:8557251-8557270 Msa0835240:CDS 50.0%
!! GGTTCTGAAGGTTCTGGTAC+CGG - chr5_4:8557273-8557292 None:intergenic 50.0%
!! AGCTAGGGTTCTGAAGGTTC+TGG - chr5_4:8557279-8557298 None:intergenic 50.0%
! TGCTGAAGCTAGGGTTCTGA+AGG - chr5_4:8557285-8557304 None:intergenic 50.0%
ACATGGCCGAAAGTAGTCCA+TGG - chr5_4:8557371-8557390 None:intergenic 50.0%
TTCGGCCATGTTGCATGCAA+TGG + chr5_4:8557380-8557399 Msa0835240:CDS 50.0%
!! AAAAAGCGTTGGCCACTGAG+TGG - chr5_4:8557471-8557490 None:intergenic 50.0%
!!! GCCAACGCTTTTTGAAGCGT+TGG + chr5_4:8557478-8557497 Msa0835240:CDS 50.0%
! GCCAACGCTTCAAAAAGCGT+TGG - chr5_4:8557482-8557501 None:intergenic 50.0%
ATGGTGGTTAAACGGAGGTG+CGG - chr5_4:8557524-8557543 None:intergenic 50.0%
! TGACGATGGTGGTTAAACGG+AGG - chr5_4:8557529-8557548 None:intergenic 50.0%
TTAACCACCATCGTCACTGC+TGG + chr5_4:8557533-8557552 Msa0835240:CDS 50.0%
TAGCTTCGTTGATCGCGTTG+CGG + chr5_4:8557789-8557808 Msa0835240:CDS 50.0%
TTGAAAGGGAAGCTGGGAGA+GGG + chr5_4:8557972-8557991 Msa0835240:CDS 50.0%
!! TGATTTGGTGGCATGTGCTC+CGG + chr5_4:8558000-8558019 Msa0835240:CDS 50.0%
GATTGAACGGAGAGAAACGG+CGG + chr5_4:8558030-8558049 Msa0835240:CDS 50.0%
TGGTGGAGGGTTTAATACGG+TGG + chr5_4:8558075-8558094 Msa0835240:CDS 50.0%
! TGAGGAGGTATAAGGAAGGG+TGG + chr5_4:8558137-8558156 Msa0835240:CDS 50.0%
AGGAAGGGTGGTCGATGATA+CGG + chr5_4:8558149-8558168 Msa0835240:CDS 50.0%
CTTGACGTGGAAGGAACAAC+CGG + chr5_4:8558192-8558211 Msa0835240:CDS 50.0%
!! TGTTGGTGGTGGTGTTGGTG+TGG - chr5_4:8556792-8556811 None:intergenic 55.0%
!! TAGCTCGTGCTGTTTCGAGG+AGG - chr5_4:8557118-8557137 None:intergenic 55.0%
!! GAAGGTTCTGGTACCGGCTT+CGG - chr5_4:8557267-8557286 None:intergenic 55.0%
TGGCGCCATTGCATGCAACA+TGG - chr5_4:8557388-8557407 None:intergenic 55.0%
! CGGAGTATCATCAGCACGAG+TGG - chr5_4:8557504-8557523 None:intergenic 55.0%
AGAACCACCAGCAGTGACGA+TGG - chr5_4:8557543-8557562 None:intergenic 55.0%
AAGCTATCAACGCGTCGCGA+CGG - chr5_4:8557775-8557794 None:intergenic 55.0%
CTCTCCCAGCTTCCCTTTCA+AGG - chr5_4:8557973-8557992 None:intergenic 55.0%
CTTGAAAGGGAAGCTGGGAG+AGG + chr5_4:8557971-8557990 Msa0835240:CDS 55.0%
CTCCGGCAGAGTCGATTGAA+CGG + chr5_4:8558017-8558036 Msa0835240:CDS 55.0%
! CTCCGTTCAATCGACTCTGC+CGG - chr5_4:8558022-8558041 None:intergenic 55.0%
AAACGGCGGTGAGATGGTCA+CGG + chr5_4:8558044-8558063 Msa0835240:CDS 55.0%
AGATGGTCACGGCGTTTGCA+TGG + chr5_4:8558055-8558074 Msa0835240:CDS 55.0%
GAAGGAACAACCGGTGGTGT+GGG + chr5_4:8558201-8558220 Msa0835240:CDS 55.0%
!! TGTTGGTGGTGGTGTTGGTG+TGG - chr5_4:8556792-8556811 None:intergenic 55.0%
!! TAGCTCGTGCTGTTTCGAGG+AGG - chr5_4:8557118-8557137 None:intergenic 55.0%
!! GAAGGTTCTGGTACCGGCTT+CGG - chr5_4:8557267-8557286 None:intergenic 55.0%
TGGCGCCATTGCATGCAACA+TGG - chr5_4:8557388-8557407 None:intergenic 55.0%
! CGGAGTATCATCAGCACGAG+TGG - chr5_4:8557504-8557523 None:intergenic 55.0%
AGAACCACCAGCAGTGACGA+TGG - chr5_4:8557543-8557562 None:intergenic 55.0%
AAGCTATCAACGCGTCGCGA+CGG - chr5_4:8557775-8557794 None:intergenic 55.0%
CTCTCCCAGCTTCCCTTTCA+AGG - chr5_4:8557973-8557992 None:intergenic 55.0%
CTTGAAAGGGAAGCTGGGAG+AGG + chr5_4:8557971-8557990 Msa0835240:CDS 55.0%
CTCCGGCAGAGTCGATTGAA+CGG + chr5_4:8558017-8558036 Msa0835240:CDS 55.0%
! CTCCGTTCAATCGACTCTGC+CGG - chr5_4:8558022-8558041 None:intergenic 55.0%
AAACGGCGGTGAGATGGTCA+CGG + chr5_4:8558044-8558063 Msa0835240:CDS 55.0%
AGATGGTCACGGCGTTTGCA+TGG + chr5_4:8558055-8558074 Msa0835240:CDS 55.0%
GAAGGAACAACCGGTGGTGT+GGG + chr5_4:8558201-8558220 Msa0835240:CDS 55.0%
!! GGTGGTGGTGTTGGTGTGGT+TGG - chr5_4:8556788-8556807 None:intergenic 60.0%
! GGGTGTGAGTGATGGTGTGG+GGG - chr5_4:8557039-8557058 None:intergenic 60.0%
! GGGGTGTGAGTGATGGTGTG+GGG - chr5_4:8557040-8557059 None:intergenic 60.0%
! GGGGGTGTGAGTGATGGTGT+GGG - chr5_4:8557041-8557060 None:intergenic 60.0%
! AGGGGGTGTGAGTGATGGTG+TGG - chr5_4:8557042-8557061 None:intergenic 60.0%
ACCACCATCGTCACTGCTGG+TGG + chr5_4:8557536-8557555 Msa0835240:CDS 60.0%
ACCACCAGCAGTGACGATGG+TGG - chr5_4:8557540-8557559 None:intergenic 60.0%
GCGGCCGAGGATAGTAACAG+TGG + chr5_4:8557808-8557827 Msa0835240:CDS 60.0%
GGAGAGAAACGGCGGTGAGA+TGG + chr5_4:8558038-8558057 Msa0835240:CDS 60.0%
TGGTCACGGCGTTTGCATGG+TGG + chr5_4:8558058-8558077 Msa0835240:CDS 60.0%
TCACGGCGTTTGCATGGTGG+AGG + chr5_4:8558061-8558080 Msa0835240:CDS 60.0%
! CACGGCGTTTGCATGGTGGA+GGG + chr5_4:8558062-8558081 Msa0835240:CDS 60.0%
GACGTGGAAGGAACAACCGG+TGG + chr5_4:8558195-8558214 Msa0835240:CDS 60.0%
GGAAGGAACAACCGGTGGTG+TGG + chr5_4:8558200-8558219 Msa0835240:CDS 60.0%
!! GGTGGTGGTGTTGGTGTGGT+TGG - chr5_4:8556788-8556807 None:intergenic 60.0%
! GGGTGTGAGTGATGGTGTGG+GGG - chr5_4:8557039-8557058 None:intergenic 60.0%
! GGGGTGTGAGTGATGGTGTG+GGG - chr5_4:8557040-8557059 None:intergenic 60.0%
! GGGGGTGTGAGTGATGGTGT+GGG - chr5_4:8557041-8557060 None:intergenic 60.0%
! AGGGGGTGTGAGTGATGGTG+TGG - chr5_4:8557042-8557061 None:intergenic 60.0%
ACCACCATCGTCACTGCTGG+TGG + chr5_4:8557536-8557555 Msa0835240:CDS 60.0%
ACCACCAGCAGTGACGATGG+TGG - chr5_4:8557540-8557559 None:intergenic 60.0%
GCGGCCGAGGATAGTAACAG+TGG + chr5_4:8557808-8557827 Msa0835240:CDS 60.0%
GGAGAGAAACGGCGGTGAGA+TGG + chr5_4:8558038-8558057 Msa0835240:CDS 60.0%
TGGTCACGGCGTTTGCATGG+TGG + chr5_4:8558058-8558077 Msa0835240:CDS 60.0%
TCACGGCGTTTGCATGGTGG+AGG + chr5_4:8558061-8558080 Msa0835240:CDS 60.0%
! CACGGCGTTTGCATGGTGGA+GGG + chr5_4:8558062-8558081 Msa0835240:CDS 60.0%
GACGTGGAAGGAACAACCGG+TGG + chr5_4:8558195-8558214 Msa0835240:CDS 60.0%
GGAAGGAACAACCGGTGGTG+TGG + chr5_4:8558200-8558219 Msa0835240:CDS 60.0%
! GGTGGAGGGGGTGTGAGTGA+TGG - chr5_4:8557047-8557066 None:intergenic 65.0%
! CATGCACTGGCCCACACCAC+CGG - chr5_4:8558214-8558233 None:intergenic 65.0%
TGGTGTGGGCCAGTGCATGG+AGG + chr5_4:8558215-8558234 Msa0835240:CDS 65.0%
! GGTGGAGGGGGTGTGAGTGA+TGG - chr5_4:8557047-8557066 None:intergenic 65.0%
! CATGCACTGGCCCACACCAC+CGG - chr5_4:8558214-8558233 None:intergenic 65.0%
TGGTGTGGGCCAGTGCATGG+AGG + chr5_4:8558215-8558234 Msa0835240:CDS 65.0%
CGTTGATCGCGTTGCGGCCG+AGG + chr5_4:8557795-8557814 Msa0835240:CDS 70.0%
CGGTGGTGTGGGCCAGTGCA+TGG + chr5_4:8558212-8558231 Msa0835240:CDS 70.0%
CGTTGATCGCGTTGCGGCCG+AGG + chr5_4:8557795-8557814 Msa0835240:CDS 70.0%
CGGTGGTGTGGGCCAGTGCA+TGG + chr5_4:8558212-8558231 Msa0835240:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 8556756 8558243 8556756 ID=Msa0835240;Name=Msa0835240
chr5_4 mRNA 8556756 8558243 8556756 ID=Msa0835240-mRNA-1;Parent=Msa0835240;Name=Msa0835240-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|495
chr5_4 exon 8556756 8558243 8556756 ID=Msa0835240-mRNA-1:exon:3759;Parent=Msa0835240-mRNA-1
chr5_4 CDS 8556756 8558243 8556756 ID=Msa0835240-mRNA-1:cds;Parent=Msa0835240-mRNA-1
Gene Sequence

>Msa0835240

ATGGATACATTGTTTAGACTTGTAAGCTTCCAACCACACCAACACCACCACCAACAACAACAACAAGAAGATCAATCTCTCAACTCCACCACCAGCAGAACAACAACATCAAGTAGCTCTCGATCCTCAAAACAAAACTATCACCAATATTACAATCCACAAGAAGTTGAAGAAGAATGCTTCAACTTTTTCATGGATGAAGAAGACCTTTCATCATCTTCTTCCAAACACTACTATCCTTATAGTCAACCTCAAACCTCAAACACCATTACTACAAACACCCCCACACCATCACTCACACCCCCTCCACCAACAACTGATTTCACCTTTGAATTAACTGGAAAGTGGGCCAACAACATCCTCCTCGAAACAGCACGAGCTATAGCAGAAAAAAACAGCACTCGTTTACAACAACTCATGTGGATGCTCAACGAACTAAGCTCCCCTTATGGTGACATAGAACAAAAACTAGCTGCATATTTTCTCCAAGCCTTATTCTCTCGAATGACCGAAGCCGGTACCAGAACCTTCAGAACCCTAGCTTCAGCATCAGAAAAAACTTGCTCATTCGAATCAACACGAAAAACAGTCCTAAAATTCCAAGAAGTTAGTCCATGGACTACTTTCGGCCATGTTGCATGCAATGGCGCCATTTTAGAAGCCTTCGAAGGTGATTCTAAGTTGCACATAATTGATATTAGTAACACTTATTCCACTCAGTGGCCAACGCTTTTTGAAGCGTTGGCCACTCGTGCTGATGATACTCCGCACCTCCGTTTAACCACCATCGTCACTGCTGGTGGTTCTGTTCAAAAAGTTATGAAGGAAATTGGTGCTAGAATGGAAAAATTCGCTAGACTTATGGGAGTTCCATTTAAATTCAACGTTATTCATCACTCGGGCGATCTTTCTGATTTAAATTTTTTGGATTTAGATATTAAAGAAGATGAAGCTTTAGCTGTTAATTGTGTTAATGCTTTGCATTCAGTAACAGTTGGTAATGATAACGGTAATAACCGTCGCGACGCGTTGATAGCTTCGTTGATCGCGTTGCGGCCGAGGATAGTAACAGTGGTTGAAGAGGAGGCTGATTTAAATTTTGGTAATGATGGATATGAATTTGTGAATGGTTTTGAAGAGTGTTTGAGATGGTTTAGGGTTTACTTTGAGGCATTAGAAGAGAGTTTTCCGAAGACGAGTAACGAGCGTTTGATCCTTGAAAGGGAAGCTGGGAGAGGGATAGTTGATTTGGTGGCATGTGCTCCGGCAGAGTCGATTGAACGGAGAGAAACGGCGGTGAGATGGTCACGGCGTTTGCATGGTGGAGGGTTTAATACGGTGGCGTTTAGTGAGGAAGTTTGTGATGATGTTAGGGCTTTGTTGAGGAGGTATAAGGAAGGGTGGTCGATGATACGGTGTAATGATGCCGGAATATTCTTGACGTGGAAGGAACAACCGGTGGTGTGGGCCAGTGCATGGAGGCCTTGA
Protein sequence

>Msa0835240

MDTLFRLVSFQPHQHHHQQQQQEDQSLNSTTSRTTTSSSSRSSKQNYHQYYNPQEVEEECFNFFMDEEDLSSSSSKHYYPYSQPQTSNTITTNTPTPSLTPPPPTTDFTFELTGKWANNILLETARAIAEKNSTRLQQLMWMLNELSSPYGDIEQKLAAYFLQALFSRMTEAGTRTFRTLASASEKTCSFESTRKTVLKFQEVSPWTTFGHVACNGAILEAFEGDSKLHIIDISNTYSTQWPTLFEALATRADDTPHLRLTTIVTAGGSVQKVMKEIGARMEKFARLMGVPFKFNVIHHSGDLSDLNFLDLDIKEDEALAVNCVNALHSVTVGNDNGNNRRDALIASLIALRPRIVTVVEEEADLNFGNDGYEFVNGFEECLRWFRVYFEALEESFPKTSNERLILEREAGRGIVDLVACAPAESIERRETAVRWSRRLHGGGFNTVAFSEEVCDDVRALLRRYKEGWSMIRCNDAGIFLTWKEQPVVWASAWRP*