AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0720170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0720170 MtrunA17_Chr2g0282771 84.866 707 32 4 17 648 1089 1795 0.0 1140
Msa0720170 MtrunA17_Chr4g0046591 60.206 681 202 11 17 633 1028 1703 0.0 717
Msa0720170 MtrunA17_Chr4g0040511 35.322 637 322 18 20 584 1074 1692 1.23e-98 331
Msa0720170 MtrunA17_Chr8g0389091 28.097 331 212 11 41 361 736 1050 2.04e-29 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0720170 AT5G44180.3 44.524 420 168 8 35 416 1034 1426 1.67e-96 325
Msa0720170 AT5G44180.1 44.524 420 168 8 35 416 1034 1426 1.67e-96 325
Msa0720170 AT1G28420.1 42.485 499 222 12 48 517 1078 1540 2.16e-93 317
Msa0720170 AT5G44180.2 67.442 86 25 1 35 117 1034 1119 1.68e-28 122
Msa0720170 AT5G44180.2 39.370 127 51 1 316 416 1113 1239 3.88e-17 86.7
Msa0720170 AT4G12750.1 27.760 317 207 11 63 370 747 1050 6.13e-25 111

Find 221 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGTATTGTTCTGAATTT+TGG 0.128459 5_1:-16108307 None:intergenic
GGTAAATGTAAAAGAAAATT+TGG 0.144383 5_1:+16108346 Msa0720170:five_prime_UTR
CAATTTATCCAAAGATATTA+TGG 0.195173 5_1:+16110087 Msa0720170:CDS
CTGAAGCATTCGGAACAATT+TGG 0.199999 5_1:+16110311 Msa0720170:CDS
AAAATCCAGATATTTGAAAA+TGG 0.211386 5_1:+16108173 Msa0720170:five_prime_UTR
TGGAATATGATCATGGAAAT+TGG 0.222823 5_1:+16111469 Msa0720170:CDS
TGAAAATCTTGATGATAATA+TGG 0.280956 5_1:+16108503 Msa0720170:five_prime_UTR
AGAATGTTCCATAATATCTT+TGG 0.289710 5_1:-16110095 None:intergenic
CGATAATATGAATATTAGTT+TGG 0.298203 5_1:+16108325 Msa0720170:five_prime_UTR
GCTGCAAATTTAACTGTTCC+TGG 0.298642 5_1:-16107831 None:intergenic
CTTGTCAGAAATCTGGTCTT+CGG 0.300420 5_1:+16107991 Msa0720170:intron
CCAGTGCCCGAGTGCCATTC+AGG 0.304676 5_1:+16109728 Msa0720170:CDS
GTTGCTTCTTTCTCCTCAAC+AGG 0.312467 5_1:-16110764 None:intergenic
CTCTAGTACATTCAAACCTT+TGG 0.314270 5_1:-16107880 None:intergenic
GTGCAGAAGGAGTTCCTCTT+TGG 0.318500 5_1:-16108986 None:intergenic
CTCAAGCTTTACGTAGTTGA+TGG 0.322653 5_1:-16110732 None:intergenic
TTAGATTTCCCATCACTTGC+TGG 0.328353 5_1:+16108849 Msa0720170:five_prime_UTR
TCTAGTACATTCAAACCTTT+GGG 0.336654 5_1:-16107879 None:intergenic
CTTTCTTGCTTCTGAAAGAA+TGG 0.337761 5_1:-16108150 None:intergenic
TGATGCTTCAGGTGTCTTGC+TGG 0.339404 5_1:-16108024 None:intergenic
GAGTTGTCCGGACCTGTGCC+TGG 0.340287 5_1:-16109051 None:intergenic
AAATCCCTATCCCATCTGTT+AGG 0.342001 5_1:-16111516 None:intergenic
CACTGGATTCACGCGGGATC+AGG 0.342944 5_1:+16109573 Msa0720170:CDS
ATCGGTTACGCCTGCGGTCC+TGG 0.348401 5_1:-16109182 None:intergenic
TAACAGATGGGATAGGGATT+TGG 0.350231 5_1:+16111518 Msa0720170:CDS
GTAAATGTAAAAGAAAATTT+GGG 0.361916 5_1:+16108347 Msa0720170:five_prime_UTR
CATCATTTGACGGAAATGTT+TGG 0.362870 5_1:-16110027 None:intergenic
TGGGAGTCTTCCATAAAATA+AGG 0.365324 5_1:-16109989 None:intergenic
TCTAGTAATGATCCTGGTTC+TGG 0.366872 5_1:+16109228 Msa0720170:CDS
ACCGCAGGCGTAACCGATAT+TGG 0.370287 5_1:+16109187 Msa0720170:CDS
CAGGTGTCTTGCTGGTTGTC+AGG 0.370707 5_1:-16108016 None:intergenic
TTTACCAAATCATCGTCTTC+AGG 0.370784 5_1:-16108269 None:intergenic
TTGTCAGAAATCTGGTCTTC+GGG 0.377803 5_1:+16107992 Msa0720170:intron
GACACCTGAAGCATCAATTT+CGG 0.383323 5_1:+16108031 Msa0720170:five_prime_UTR
CAAGCAACTGGTTGGTTGTC+TGG 0.385098 5_1:-16108465 None:intergenic
AAATTCTCTGTTAGGCTTGA+AGG 0.387266 5_1:-16110928 None:intergenic
TACTGGCCGTCTAAACATTA+AGG 0.390121 5_1:+16111100 Msa0720170:CDS
TACGCCTGCGGTCCTGGCCA+AGG 0.390990 5_1:-16109176 None:intergenic
AGAGGGACAATTGGCATCCT+TGG 0.392635 5_1:-16108435 None:intergenic
GGATCCTGAAGACGATGATT+TGG 0.395309 5_1:+16108265 Msa0720170:five_prime_UTR
GGGCGAGGAGCTAGTGATAT+TGG 0.396116 5_1:+16111032 Msa0720170:CDS
TGCATGCACAATATTCAAAA+AGG 0.403309 5_1:+16109085 Msa0720170:CDS
AGAGCTTGCAGAGAAGATTC+AGG 0.403864 5_1:+16107899 Msa0720170:five_prime_UTR
TAATTTCTGACTTAGATTCT+CGG 0.403941 5_1:-16111126 None:intergenic
TACCCCTTGGCCAGGACCGC+AGG 0.404528 5_1:+16109172 Msa0720170:CDS
TAGACGGCCAGTATCTTGTT+TGG 0.406576 5_1:-16111090 None:intergenic
TCTGAGGACAAGATTGCTTA+AGG 0.408514 5_1:+16110123 Msa0720170:CDS
GATCATGGAAATTGGGAGAT+AGG 0.409401 5_1:+16111477 Msa0720170:CDS
TACAATTCCAAACAAGATAC+TGG 0.409805 5_1:+16111083 Msa0720170:CDS
AAATCCAGATATTTGAAAAT+GGG 0.416183 5_1:+16108174 Msa0720170:five_prime_UTR
TTTCATGTTCTCCCTCTTGA+GGG 0.419605 5_1:+16107855 Msa0720170:five_prime_UTR
CCCGAGTGCCATTCAGGATC+TGG 0.422531 5_1:+16109734 Msa0720170:CDS
GCAACCGAAATTGATGCTTC+AGG 0.423831 5_1:-16108035 None:intergenic
CTGAATAGAGTAGTGAATGT+AGG 0.424161 5_1:+16111642 Msa0720170:CDS
CAAGCCTAACAGAGAATTTG+AGG 0.425911 5_1:+16110932 Msa0720170:CDS
CTCCCTCTTGAGGGCCCCAA+AGG 0.426193 5_1:+16107864 Msa0720170:five_prime_UTR
TAAGGTCCTGCTGTCATGTA+TGG 0.429429 5_1:+16110141 Msa0720170:CDS
CCTGAATGGCACTCGGGCAC+TGG 0.430744 5_1:-16109728 None:intergenic
CTCTACAACAGACGAATTGT+TGG 0.431310 5_1:+16110584 Msa0720170:CDS
ACATCAATATTAACAACATT+AGG 0.432077 5_1:+16108934 Msa0720170:five_prime_UTR
AATGCTCTTGTTGCATTGGT+TGG 0.434333 5_1:+16108612 Msa0720170:five_prime_UTR
GGAATATGATCATGGAAATT+GGG 0.434917 5_1:+16111470 Msa0720170:CDS
TCTACAACAGACGAATTGTT+GGG 0.435097 5_1:+16110585 Msa0720170:CDS
GAAATCGATGAAAGCAAGTC+TGG 0.440423 5_1:+16108525 Msa0720170:five_prime_UTR
AATCCCTATCCCATCTGTTA+GGG 0.444940 5_1:-16111515 None:intergenic
GTCATTTCTACACTTCGTAA+TGG 0.445805 5_1:+16107733 Msa0720170:five_prime_UTR
ATGCAACAAACTGCCAATAT+CGG 0.446056 5_1:-16109200 None:intergenic
TTTAGATTTCCACAAGCAAC+TGG 0.446414 5_1:-16108477 None:intergenic
AAACACACATTTCAATGTGT+TGG 0.447832 5_1:+16110064 Msa0720170:CDS
GAAATTAAAACAGCAGGGAC+AGG 0.447944 5_1:+16111142 Msa0720170:CDS
AGCTGTTGAAGATTTGTTGC+TGG 0.448006 5_1:+16111226 Msa0720170:CDS
AACTGTTTCTCAGGATCGAT+TGG 0.451863 5_1:+16108743 Msa0720170:intron
ACAGGGAACACAAGGACAAG+GGG 0.454229 5_1:+16111160 Msa0720170:CDS
GCTGCCTCAAATTCTCTGTT+AGG 0.455149 5_1:-16110936 None:intergenic
TGACGGAAATGTTTGGTGGC+AGG 0.456417 5_1:-16110020 None:intergenic
CTTTGATTTCCCTCCACAGC+AGG 0.456515 5_1:-16108900 None:intergenic
ATTTCATGTTCTCCCTCTTG+AGG 0.457194 5_1:+16107854 Msa0720170:five_prime_UTR
TCTGGAGAACCATGGGTTCA+AGG 0.457333 5_1:+16108543 Msa0720170:five_prime_UTR
AGATGTTATGAGTGAAGGTT+CGG 0.457471 5_1:+16111611 Msa0720170:CDS
ACCATGGGTTCAAGGACTTA+CGG 0.459012 5_1:+16108551 Msa0720170:five_prime_UTR
GCATCCTTGGAACCATTCTC+AGG 0.461047 5_1:-16108422 None:intergenic
ATTAGCAGAAGGTTTAACTT+TGG 0.465048 5_1:-16110982 None:intergenic
GTAGCTCATGATTTCTGCCC+AGG 0.468923 5_1:+16109033 Msa0720170:CDS
TAATATTGATGTCGAGCAAT+GGG 0.469453 5_1:-16108923 None:intergenic
TCATCCTTCAAAATTGAGCT+TGG 0.481622 5_1:+16109809 Msa0720170:CDS
ACGCCTGCGGTCCTGGCCAA+GGG 0.484182 5_1:-16109175 None:intergenic
TCTGTTCTCTCTGAAGCATT+CGG 0.485528 5_1:+16110301 Msa0720170:CDS
TTTGAAAATGGGTTTGTAGC+TGG 0.485672 5_1:+16108185 Msa0720170:five_prime_UTR
GGTGACAGAAGAGGGACAAT+TGG 0.486340 5_1:-16108444 None:intergenic
TGAATAGAGTAGTGAATGTA+GGG 0.488159 5_1:+16111643 Msa0720170:CDS
GTTCGTACACGATATGTTGA+TGG 0.491663 5_1:-16108095 None:intergenic
TCGGTTGCTTTGACAAGAGA+TGG 0.494544 5_1:+16108050 Msa0720170:five_prime_UTR
AATTCTCTGTTAGGCTTGAA+GGG 0.496793 5_1:-16110927 None:intergenic
CAGAGGAGATGTATGTCTAC+AGG 0.497524 5_1:+16109145 Msa0720170:CDS
AAGTCAGAAATTAAAACAGC+AGG 0.497648 5_1:+16111136 Msa0720170:CDS
TTCAATCCGCATTATTCTTG+AGG 0.502637 5_1:+16108653 Msa0720170:five_prime_UTR
TCCACCCTTGGAACTATGAC+TGG 0.504696 5_1:-16111261 None:intergenic
GCTAATAAAATTGCACGAAG+TGG 0.504989 5_1:+16110999 Msa0720170:CDS
AAATTAAATTCATCATTTGA+CGG 0.505138 5_1:-16110037 None:intergenic
ATTTATTTCCAGCAAGTGAT+GGG 0.509213 5_1:-16108857 None:intergenic
GCCAGATCCTGAATGGCACT+CGG 0.510123 5_1:-16109735 None:intergenic
CATGATTTCTGCCCAGGCAC+AGG 0.510338 5_1:+16109039 Msa0720170:CDS
CAGCAGGGACAGGGAACACA+AGG 0.510675 5_1:+16111152 Msa0720170:CDS
TCTAAATGCTCTTGTTGCAT+TGG 0.511458 5_1:+16108608 Msa0720170:five_prime_UTR
AACTATGACTGGCTGCTGCA+TGG 0.513290 5_1:-16111250 None:intergenic
AGATGGGATAGGGATTTGGT+TGG 0.513510 5_1:+16111522 Msa0720170:CDS
ATCTGACTCTGAGAGTTGTC+CGG 0.518778 5_1:-16109063 None:intergenic
ACAAAGATCCTGCCAGAACC+AGG 0.519239 5_1:-16109240 None:intergenic
TTGAACATCATGATGGCAGT+TGG 0.522513 5_1:+16109262 Msa0720170:CDS
TCCGTAAGTCCTTGAACCCA+TGG 0.523687 5_1:-16108552 None:intergenic
CGGTGACCTTAATGTTTAGA+CGG 0.524832 5_1:-16111106 None:intergenic
GTCTACAGGTCTCTACCCCT+TGG 0.527484 5_1:+16109159 Msa0720170:CDS
TTGAAGGGAGTATATCTTGA+TGG 0.527833 5_1:-16110912 None:intergenic
TTTCTGCCCAGGCACAGGTC+CGG 0.528079 5_1:+16109044 Msa0720170:CDS
CATTTGACGGAAATGTTTGG+TGG 0.528443 5_1:-16110024 None:intergenic
TCTCCCCTAACAGATGGGAT+AGG 0.529720 5_1:+16111511 Msa0720170:CDS
AGAATGGACTCAGCATCTGC+AGG 0.531873 5_1:-16108134 None:intergenic
AACCAGAGTTCAACTCATGA+AGG 0.532603 5_1:-16109772 None:intergenic
AATTTATTTCCAGCAAGTGA+TGG 0.534389 5_1:-16108858 None:intergenic
CTCCCCTAACAGATGGGATA+GGG 0.534441 5_1:+16111512 Msa0720170:CDS
ACCCTTCATGAGTTGAACTC+TGG 0.536420 5_1:+16109770 Msa0720170:CDS
AACATCATGATGGCAGTTGG+AGG 0.538714 5_1:+16109265 Msa0720170:CDS
CATACATCTCCTCTGCTATG+TGG 0.538744 5_1:-16109137 None:intergenic
GCTGTTGAAGATTTGTTGCT+GGG 0.539538 5_1:+16111227 Msa0720170:CDS
TTCACTCATAACATCTTCCT+CGG 0.541196 5_1:-16111604 None:intergenic
GTAATGATCCTGGTTCTGGC+AGG 0.541880 5_1:+16109232 Msa0720170:CDS
TCTACTTATTGAAATGCCAT+TGG 0.542096 5_1:+16109011 Msa0720170:CDS
AGATGAAGAAGACAATGAAG+AGG 0.542565 5_1:+16111575 Msa0720170:CDS
GGACAGGGAACACAAGGACA+AGG 0.543077 5_1:+16111158 Msa0720170:CDS
AAACCTTTGGGGCCCTCAAG+AGG 0.543333 5_1:-16107867 None:intergenic
GCCAATATCGGTTACGCCTG+CGG 0.543579 5_1:-16109188 None:intergenic
CACTGCTTGAGCATCATCAT+CGG 0.546864 5_1:-16111421 None:intergenic
CACTGCTTGAGCATCATCAT+CGG 0.546864 5_1:-16111448 None:intergenic
TCTGCTTCTAGTAATGATCC+TGG 0.547326 5_1:+16109222 Msa0720170:CDS
CAGGTCTCTACCCCTTGGCC+AGG 0.550807 5_1:+16109164 Msa0720170:CDS
TGAGAGACTTGAGCCTGTTG+AGG 0.551060 5_1:+16110751 Msa0720170:CDS
ACTGGATTCACGCGGGATCA+GGG 0.551277 5_1:+16109574 Msa0720170:CDS
AAAAGAAAGCTGCCTTGAGA+AGG 0.555107 5_1:+16109846 Msa0720170:CDS
CAAGCAGTGGAATATGATCA+TGG 0.556622 5_1:+16111462 Msa0720170:CDS
TTAATATTGATGTCGAGCAA+TGG 0.557741 5_1:-16108924 None:intergenic
AGGAGCTAGTGATATTGGAC+GGG 0.557907 5_1:+16111037 Msa0720170:CDS
ATACATCTCCTCTGCTATGT+GGG 0.559503 5_1:-16109136 None:intergenic
GACAACCAACCAGTTGCTTG+TGG 0.567270 5_1:+16108468 Msa0720170:five_prime_UTR
CGGTGTCTCCCCTAACAGAT+GGG 0.568914 5_1:+16111506 Msa0720170:CDS
ATCTTTGTTGAACATCATGA+TGG 0.570451 5_1:+16109255 Msa0720170:CDS
GCAGCAGCTCTTGATCATGA+TGG 0.570686 5_1:+16110954 Msa0720170:CDS
TAGGACTGCCAGATCCTGAA+TGG 0.571093 5_1:-16109742 None:intergenic
TCTTGTTGCATTGGTTGGTG+TGG 0.571797 5_1:+16108617 Msa0720170:five_prime_UTR
TGTACGAACTGCATTTAGAC+AGG 0.572915 5_1:+16108109 Msa0720170:five_prime_UTR
AGTTGTCCGGACCTGTGCCT+GGG 0.572957 5_1:-16109050 None:intergenic
AATAGTAACTGCTGAAGAGG+TGG 0.573085 5_1:+16111368 Msa0720170:intron
ACGGTGTCTCCCCTAACAGA+TGG 0.573565 5_1:+16111505 Msa0720170:CDS
CAGGGAACACAAGGACAAGG+GGG 0.575472 5_1:+16111161 Msa0720170:CDS
AACCTTTGGGGCCCTCAAGA+GGG 0.579011 5_1:-16107866 None:intergenic
GATTTCCACAAGCAACTGGT+TGG 0.580354 5_1:-16108473 None:intergenic
TTTATGTGCAATAAAATTTG+GGG 0.580757 5_1:+16109919 Msa0720170:CDS
CCAGATCCTGAATGGCACTC+GGG 0.581063 5_1:-16109734 None:intergenic
GACAGGGAACACAAGGACAA+GGG 0.581330 5_1:+16111159 Msa0720170:CDS
GAGGCTTATTGATTCTGAAG+AGG 0.583561 5_1:+16109284 Msa0720170:CDS
CAATAAGTAGACTGTGCAGA+AGG 0.584772 5_1:-16108999 None:intergenic
AAAGAGATGCAAACCTCAGG+TGG 0.585260 5_1:+16109943 Msa0720170:CDS
GTTATGAGTGAAGGTTCGGA+TGG 0.585945 5_1:+16111615 Msa0720170:CDS
GATTTGGTAAATCCTTCAAG+TGG 0.586869 5_1:+16108281 Msa0720170:five_prime_UTR
TCGAGACACAGGTTAACTCC+AGG 0.587615 5_1:+16107813 Msa0720170:five_prime_UTR
AACCTGTGTCTCGATCTTCG+AGG 0.587861 5_1:-16107804 None:intergenic
TGATGTCAAAAGAATATCAA+AGG 0.588563 5_1:-16109552 None:intergenic
TCACCTCCATACATGACAGC+AGG 0.588857 5_1:-16110147 None:intergenic
AGCAGCCAGTCATAGTTCCA+AGG 0.589263 5_1:+16111256 Msa0720170:CDS
AACTCATGAAGGGTACTGCT+AGG 0.590927 5_1:-16109761 None:intergenic
AGTGGAAACAAACGTGGGCG+AGG 0.591012 5_1:+16111017 Msa0720170:CDS
GAGGAAGATGTTATGAGTGA+AGG 0.593223 5_1:+16111606 Msa0720170:CDS
GGTTGTCTGGTGACAGAAGA+GGG 0.595103 5_1:-16108452 None:intergenic
TTGGTTGGTGTGGCAAATGA+AGG 0.598262 5_1:+16108627 Msa0720170:five_prime_UTR
AAGCAAGTCTGGAGAACCAT+GGG 0.599961 5_1:+16108536 Msa0720170:five_prime_UTR
AAAATCTTGATACCTTCTCA+AGG 0.600897 5_1:-16109858 None:intergenic
CAATGAAGAGGAAGATTCCG+AGG 0.601580 5_1:+16111587 Msa0720170:CDS
AGAAAGTGATGAAGTTGATG+AGG 0.602540 5_1:+16108244 Msa0720170:five_prime_UTR
GGTAAAGAGATGCAAACCTC+AGG 0.602721 5_1:+16109940 Msa0720170:CDS
AGTCAGAAATTAAAACAGCA+GGG 0.604689 5_1:+16111137 Msa0720170:CDS
CGCCTGCGGTCCTGGCCAAG+GGG 0.612330 5_1:-16109174 None:intergenic
GACACATATCCTGCTGTGGA+GGG 0.615224 5_1:+16108891 Msa0720170:five_prime_UTR
AAATTAAAACAGCAGGGACA+GGG 0.615318 5_1:+16111143 Msa0720170:CDS
CGATGATGATGCTCAAGCAG+TGG 0.616148 5_1:+16111422 Msa0720170:CDS
CGATGATGATGCTCAAGCAG+TGG 0.616148 5_1:+16111449 Msa0720170:CDS
GAGGAGCTAGTGATATTGGA+CGG 0.617297 5_1:+16111036 Msa0720170:CDS
GCAGCCAGTCATAGTTCCAA+GGG 0.618569 5_1:+16111257 Msa0720170:CDS
AAAATTGGAAGCAAAGGGGA+AGG 0.621545 5_1:+16109674 Msa0720170:CDS
AGACACATATCCTGCTGTGG+AGG 0.621953 5_1:+16108890 Msa0720170:five_prime_UTR
AATTCACCTCAAGAATAATG+CGG 0.623958 5_1:-16108659 None:intergenic
GAGAATAGTAACTGCTGAAG+AGG 0.625994 5_1:+16111365 Msa0720170:intron
CTCCTCGAAGATCGAGACAC+AGG 0.626392 5_1:+16107802 Msa0720170:five_prime_UTR
TGATGATAATGCTCAAGCAG+TGG 0.630034 5_1:+16111395 Msa0720170:CDS
GTTGAGTAAATCATCATCCG+TGG 0.630562 5_1:+16110366 Msa0720170:CDS
TTCAGCAGCCGTAACTGCAG+TGG 0.630745 5_1:+16107756 Msa0720170:five_prime_UTR
ATCACTGCAGAAAACCAAAG+AGG 0.635491 5_1:+16108972 Msa0720170:CDS
TGGAATGAGTGATGAAGATG+TGG 0.636934 5_1:+16111542 Msa0720170:CDS
CAGAAATCATGAGCTACCAA+TGG 0.637579 5_1:-16109027 None:intergenic
TTATATTTCCCACATAGCAG+AGG 0.640331 5_1:+16109128 Msa0720170:CDS
AACCTGTAAAAGTTCCTCCA+CGG 0.643423 5_1:-16110383 None:intergenic
GCACGAAGTGGAAACAAACG+TGG 0.646195 5_1:+16111011 Msa0720170:CDS
ATCCCTATCCCATCTGTTAG+GGG 0.647687 5_1:-16111514 None:intergenic
AAAGCAAGTCTGGAGAACCA+TGG 0.650686 5_1:+16108535 Msa0720170:five_prime_UTR
ACCAGAGTTCAACTCATGAA+GGG 0.657800 5_1:-16109771 None:intergenic
TTGGTGGCAGGAATTGCAGT+GGG 0.660429 5_1:-16110008 None:intergenic
ATTTCACAAATATCCACCTG+AGG 0.663951 5_1:-16109956 None:intergenic
TGGAACATTCTCTTCCTCTG+AGG 0.665372 5_1:+16110107 Msa0720170:CDS
ACAAGACACATATCCTGCTG+TGG 0.665387 5_1:+16108887 Msa0720170:five_prime_UTR
TGGGTTCAAGGACTTACGGA+AGG 0.666684 5_1:+16108555 Msa0720170:five_prime_UTR
GGAGCTAGTGATATTGGACG+GGG 0.671474 5_1:+16111038 Msa0720170:CDS
TTAAGCAATCTTGTCCTCAG+AGG 0.675185 5_1:-16110121 None:intergenic
TGGTTGTCTGGTGACAGAAG+AGG 0.675190 5_1:-16108453 None:intergenic
CTAGTACATTCAAACCTTTG+GGG 0.684269 5_1:-16107878 None:intergenic
TGACATCACTGGATTCACGC+GGG 0.684391 5_1:+16109567 Msa0720170:CDS
GGTCCTGCTGTCATGTATGG+AGG 0.686922 5_1:+16110144 Msa0720170:CDS
GAGTAAATCATCATCCGTGG+AGG 0.687342 5_1:+16110369 Msa0720170:CDS
TTGACATCACTGGATTCACG+CGG 0.692134 5_1:+16109566 Msa0720170:CDS
AATAGAGTAGTGAATGTAGG+GGG 0.694338 5_1:+16111645 Msa0720170:CDS
GAATAGAGTAGTGAATGTAG+GGG 0.704575 5_1:+16111644 Msa0720170:CDS
TTTGGTGGCAGGAATTGCAG+TGG 0.721001 5_1:-16110009 None:intergenic
CACGAAGTGGAAACAAACGT+GGG 0.731398 5_1:+16111012 Msa0720170:CDS
AATTGGGAGATAGGTTACAA+CGG 0.732052 5_1:+16111486 Msa0720170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATTTACAAAAAGATT+AGG - chr5_1:16110205-16110224 None:intergenic 10.0%
!! GTAAATGTAAAAGAAAATTT+GGG + chr5_1:16108347-16108366 Msa0720170:five_prime_UTR 15.0%
!! AATACACAATATACAAAAAT+TGG + chr5_1:16109659-16109678 Msa0720170:CDS 15.0%
!!! ATTTTATGTGCAATAAAATT+TGG + chr5_1:16109917-16109936 Msa0720170:CDS 15.0%
!!! TTTTATGTGCAATAAAATTT+GGG + chr5_1:16109918-16109937 Msa0720170:CDS 15.0%
!! AAATTAAATTCATCATTTGA+CGG - chr5_1:16110040-16110059 None:intergenic 15.0%
!! TCAAAGGTAAAAATAATAAT+AGG - chr5_1:16110243-16110262 None:intergenic 15.0%
!!! AAATTTTCATCATAATCTAA+TGG + chr5_1:16110855-16110874 Msa0720170:intron 15.0%
!! AAAATCCAGATATTTGAAAA+TGG + chr5_1:16108173-16108192 Msa0720170:five_prime_UTR 20.0%
!! AAATCCAGATATTTGAAAAT+GGG + chr5_1:16108174-16108193 Msa0720170:five_prime_UTR 20.0%
!! CGATAATATGAATATTAGTT+TGG + chr5_1:16108325-16108344 Msa0720170:five_prime_UTR 20.0%
!! GGTAAATGTAAAAGAAAATT+TGG + chr5_1:16108346-16108365 Msa0720170:five_prime_UTR 20.0%
!!! TATCAAGCTTATTTTGAATA+AGG - chr5_1:16108385-16108404 None:intergenic 20.0%
!!! TGAAAATCTTGATGATAATA+TGG + chr5_1:16108503-16108522 Msa0720170:five_prime_UTR 20.0%
!! ACATCAATATTAACAACATT+AGG + chr5_1:16108934-16108953 Msa0720170:five_prime_UTR 20.0%
!! TTTATGTGCAATAAAATTTG+GGG + chr5_1:16109919-16109938 Msa0720170:CDS 20.0%
!! CAATTTATCCAAAGATATTA+TGG + chr5_1:16110087-16110106 Msa0720170:CDS 20.0%
!! TACAATAATACAATTCTCAA+AGG - chr5_1:16110259-16110278 None:intergenic 20.0%
!! ATTAACTTCAAGAATTGAAA+CGG - chr5_1:16110414-16110433 None:intergenic 20.0%
!!! TATCGTATTGTTCTGAATTT+TGG - chr5_1:16108310-16108329 None:intergenic 25.0%
! TAAAACACTAATCAAGGAAA+AGG - chr5_1:16109408-16109427 None:intergenic 25.0%
! TGATGTCAAAAGAATATCAA+AGG - chr5_1:16109555-16109574 None:intergenic 25.0%
!! TTTTCGAACATTTTCTCTAA+AGG - chr5_1:16109639-16109658 None:intergenic 25.0%
! TATACAAAAATTGGAAGCAA+AGG + chr5_1:16109668-16109687 Msa0720170:CDS 25.0%
! ATACAAAAATTGGAAGCAAA+GGG + chr5_1:16109669-16109688 Msa0720170:CDS 25.0%
! AGAATGTTCCATAATATCTT+TGG - chr5_1:16110098-16110117 None:intergenic 25.0%
!!! GTACTTACATTTTGATTTAC+AGG + chr5_1:16110276-16110295 Msa0720170:intron 25.0%
!!! TTTCATTTTCCTGTGTTATT+TGG + chr5_1:16110806-16110825 Msa0720170:intron 25.0%
!!! ATTTTTTTGCTTATTAGCAG+AGG + chr5_1:16110886-16110905 Msa0720170:intron 25.0%
! TAATTTCTGACTTAGATTCT+CGG - chr5_1:16111129-16111148 None:intergenic 25.0%
TCTAGTACATTCAAACCTTT+GGG - chr5_1:16107882-16107901 None:intergenic 30.0%
! TTCTTTTCTTGTCAGAAATC+TGG + chr5_1:16107984-16108003 Msa0720170:intron 30.0%
! CAAACCCATTTTCAAATATC+TGG - chr5_1:16108181-16108200 None:intergenic 30.0%
AATTCACCTCAAGAATAATG+CGG - chr5_1:16108662-16108681 None:intergenic 30.0%
ATTTATTTCCAGCAAGTGAT+GGG - chr5_1:16108860-16108879 None:intergenic 30.0%
AATTTATTTCCAGCAAGTGA+TGG - chr5_1:16108861-16108880 None:intergenic 30.0%
TAATATTGATGTCGAGCAAT+GGG - chr5_1:16108926-16108945 None:intergenic 30.0%
TTAATATTGATGTCGAGCAA+TGG - chr5_1:16108927-16108946 None:intergenic 30.0%
TCTACTTATTGAAATGCCAT+TGG + chr5_1:16109011-16109030 Msa0720170:CDS 30.0%
TGCATGCACAATATTCAAAA+AGG + chr5_1:16109085-16109104 Msa0720170:CDS 30.0%
ATCTTTGTTGAACATCATGA+TGG + chr5_1:16109255-16109274 Msa0720170:CDS 30.0%
CGGTAATAAAACACTAATCA+AGG - chr5_1:16109414-16109433 None:intergenic 30.0%
AAAAAAAGGCAAGCTCAAAA+CGG - chr5_1:16109434-16109453 None:intergenic 30.0%
GTATAATTCGTCCAAAACAA+TGG - chr5_1:16109473-16109492 None:intergenic 30.0%
TTATTCTACCATTCATATGC+AGG + chr5_1:16109529-16109548 Msa0720170:intron 30.0%
! TGATATTCTTTTGACATCAC+TGG + chr5_1:16109556-16109575 Msa0720170:CDS 30.0%
TACAAAAATTGGAAGCAAAG+GGG + chr5_1:16109670-16109689 Msa0720170:CDS 30.0%
AAAATCTTGATACCTTCTCA+AGG - chr5_1:16109861-16109880 None:intergenic 30.0%
!!! CTGTCTTGATCCTTATTTTA+TGG + chr5_1:16109979-16109998 Msa0720170:CDS 30.0%
AAACACACATTTCAATGTGT+TGG + chr5_1:16110064-16110083 Msa0720170:CDS 30.0%
!! CAATTTGGACAACTGATTTT+AGG + chr5_1:16110326-16110345 Msa0720170:CDS 30.0%
!! CCTTATTTGAAAAAGTGCTA+CGG + chr5_1:16110538-16110557 Msa0720170:CDS 30.0%
TTAAAAGCAGTGTGTCAAAA+AGG + chr5_1:16110609-16110628 Msa0720170:intron 30.0%
TAAAAGCAGTGTGTCAAAAA+GGG + chr5_1:16110610-16110629 Msa0720170:intron 30.0%
!!! TTATTTGGTTTTGAGCATCT+GGG + chr5_1:16110821-16110840 Msa0720170:intron 30.0%
! ATTAGCAGAAGGTTTAACTT+TGG - chr5_1:16110985-16111004 None:intergenic 30.0%
TACAATTCCAAACAAGATAC+TGG + chr5_1:16111083-16111102 Msa0720170:CDS 30.0%
AAGTCAGAAATTAAAACAGC+AGG + chr5_1:16111136-16111155 Msa0720170:CDS 30.0%
AGTCAGAAATTAAAACAGCA+GGG + chr5_1:16111137-16111156 Msa0720170:CDS 30.0%
AAGAGAACCATTAAGAAACT+TGG + chr5_1:16111283-16111302 Msa0720170:intron 30.0%
TCTTCATCCAAGTTTCTTAA+TGG - chr5_1:16111293-16111312 None:intergenic 30.0%
TAAGAAACTTGGATGAAGAA+TGG + chr5_1:16111294-16111313 Msa0720170:intron 30.0%
AAGAAACTTGGATGAAGAAT+GGG + chr5_1:16111295-16111314 Msa0720170:intron 30.0%
TGGAATATGATCATGGAAAT+TGG + chr5_1:16111469-16111488 Msa0720170:CDS 30.0%
GGAATATGATCATGGAAATT+GGG + chr5_1:16111470-16111489 Msa0720170:CDS 30.0%
TGAATAGAGTAGTGAATGTA+GGG + chr5_1:16111643-16111662 Msa0720170:CDS 30.0%
GTCATTTCTACACTTCGTAA+TGG + chr5_1:16107733-16107752 Msa0720170:five_prime_UTR 35.0%
CTAGTACATTCAAACCTTTG+GGG - chr5_1:16107881-16107900 None:intergenic 35.0%
CTCTAGTACATTCAAACCTT+TGG - chr5_1:16107883-16107902 None:intergenic 35.0%
! CTTTCTTGCTTCTGAAAGAA+TGG - chr5_1:16108153-16108172 None:intergenic 35.0%
! TTTGAAAATGGGTTTGTAGC+TGG + chr5_1:16108185-16108204 Msa0720170:five_prime_UTR 35.0%
!! AGAAAGTGATGAAGTTGATG+AGG + chr5_1:16108244-16108263 Msa0720170:five_prime_UTR 35.0%
TTTACCAAATCATCGTCTTC+AGG - chr5_1:16108272-16108291 None:intergenic 35.0%
! GATTTGGTAAATCCTTCAAG+TGG + chr5_1:16108281-16108300 Msa0720170:five_prime_UTR 35.0%
!!! GAATTTTGGTTTCCACTTGA+AGG - chr5_1:16108296-16108315 None:intergenic 35.0%
TTTAGATTTCCACAAGCAAC+TGG - chr5_1:16108480-16108499 None:intergenic 35.0%
TCTAAATGCTCTTGTTGCAT+TGG + chr5_1:16108608-16108627 Msa0720170:five_prime_UTR 35.0%
TTCAATCCGCATTATTCTTG+AGG + chr5_1:16108653-16108672 Msa0720170:five_prime_UTR 35.0%
!! ATGCTCTGAAAAAGCAAATG+TGG + chr5_1:16108775-16108794 Msa0720170:five_prime_UTR 35.0%
!! TGCTCTGAAAAAGCAAATGT+GGG + chr5_1:16108776-16108795 Msa0720170:five_prime_UTR 35.0%
TTATATTTCCCACATAGCAG+AGG + chr5_1:16109128-16109147 Msa0720170:CDS 35.0%
ATGCAACAAACTGCCAATAT+CGG - chr5_1:16109203-16109222 None:intergenic 35.0%
TACGAACAAAGACATCAATG+TGG - chr5_1:16109326-16109345 None:intergenic 35.0%
ATGCTCTGTGAGAGAAAAAA+AGG - chr5_1:16109448-16109467 None:intergenic 35.0%
TCATCCTTCAAAATTGAGCT+TGG + chr5_1:16109809-16109828 Msa0720170:CDS 35.0%
! TTTGCCAAGCTCAATTTTGA+AGG - chr5_1:16109816-16109835 None:intergenic 35.0%
! GGTATCAAGATTTTCAGAAG+TGG + chr5_1:16109867-16109886 Msa0720170:CDS 35.0%
! AAGATTTTCAGAAGTGGATG+TGG + chr5_1:16109873-16109892 Msa0720170:CDS 35.0%
ATTTCACAAATATCCACCTG+AGG - chr5_1:16109959-16109978 None:intergenic 35.0%
TGGGAGTCTTCCATAAAATA+AGG - chr5_1:16109992-16110011 None:intergenic 35.0%
CATCATTTGACGGAAATGTT+TGG - chr5_1:16110030-16110049 None:intergenic 35.0%
!!! AACTGATTTTAGGAAGCGTT+GGG + chr5_1:16110336-16110355 Msa0720170:CDS 35.0%
AAGGTCAACATCTGCAAAAT+AGG - chr5_1:16110522-16110541 None:intergenic 35.0%
!!! CCGTAGCACTTTTTCAAATA+AGG - chr5_1:16110541-16110560 None:intergenic 35.0%
TCTACAACAGACGAATTGTT+GGG + chr5_1:16110585-16110604 Msa0720170:CDS 35.0%
TGCTCAAAACCAAATAACAC+AGG - chr5_1:16110818-16110837 None:intergenic 35.0%
!!! GTTATTTGGTTTTGAGCATC+TGG + chr5_1:16110820-16110839 Msa0720170:intron 35.0%
! TTGAAGGGAGTATATCTTGA+TGG - chr5_1:16110915-16110934 None:intergenic 35.0%
AATTCTCTGTTAGGCTTGAA+GGG - chr5_1:16110930-16110949 None:intergenic 35.0%
AAATTCTCTGTTAGGCTTGA+AGG - chr5_1:16110931-16110950 None:intergenic 35.0%
! CGTGCAATTTTATTAGCAGA+AGG - chr5_1:16110996-16111015 None:intergenic 35.0%
GCTAATAAAATTGCACGAAG+TGG + chr5_1:16110999-16111018 Msa0720170:CDS 35.0%
AAATTAAAACAGCAGGGACA+GGG + chr5_1:16111143-16111162 Msa0720170:CDS 35.0%
AATTGGGAGATAGGTTACAA+CGG + chr5_1:16111486-16111505 Msa0720170:CDS 35.0%
AGATGAAGAAGACAATGAAG+AGG + chr5_1:16111575-16111594 Msa0720170:CDS 35.0%
TTCACTCATAACATCTTCCT+CGG - chr5_1:16111607-16111626 None:intergenic 35.0%
AGATGTTATGAGTGAAGGTT+CGG + chr5_1:16111611-16111630 Msa0720170:CDS 35.0%
CTGAATAGAGTAGTGAATGT+AGG + chr5_1:16111642-16111661 Msa0720170:CDS 35.0%
GAATAGAGTAGTGAATGTAG+GGG + chr5_1:16111644-16111663 Msa0720170:CDS 35.0%
AATAGAGTAGTGAATGTAGG+GGG + chr5_1:16111645-16111664 Msa0720170:CDS 35.0%
! CATTTTTGCCACTGCAGTTA+CGG - chr5_1:16107767-16107786 None:intergenic 40.0%
GCTGCAAATTTAACTGTTCC+TGG - chr5_1:16107834-16107853 None:intergenic 40.0%
ATTTCATGTTCTCCCTCTTG+AGG + chr5_1:16107854-16107873 Msa0720170:five_prime_UTR 40.0%
TTTCATGTTCTCCCTCTTGA+GGG + chr5_1:16107855-16107874 Msa0720170:five_prime_UTR 40.0%
! CTTGTCAGAAATCTGGTCTT+CGG + chr5_1:16107991-16108010 Msa0720170:intron 40.0%
! TTGTCAGAAATCTGGTCTTC+GGG + chr5_1:16107992-16108011 Msa0720170:intron 40.0%
! GACACCTGAAGCATCAATTT+CGG + chr5_1:16108031-16108050 Msa0720170:five_prime_UTR 40.0%
GTTCGTACACGATATGTTGA+TGG - chr5_1:16108098-16108117 None:intergenic 40.0%
TGTACGAACTGCATTTAGAC+AGG + chr5_1:16108109-16108128 Msa0720170:five_prime_UTR 40.0%
!!! CTGTCTTGTTTTCCTGAGAA+TGG + chr5_1:16108410-16108429 Msa0720170:five_prime_UTR 40.0%
! TTTTCCTGAGAATGGTTCCA+AGG + chr5_1:16108418-16108437 Msa0720170:five_prime_UTR 40.0%
GAAATCGATGAAAGCAAGTC+TGG + chr5_1:16108525-16108544 Msa0720170:five_prime_UTR 40.0%
! AATGCTCTTGTTGCATTGGT+TGG + chr5_1:16108612-16108631 Msa0720170:five_prime_UTR 40.0%
! AACTGTTTCTCAGGATCGAT+TGG + chr5_1:16108743-16108762 Msa0720170:intron 40.0%
TTAGATTTCCCATCACTTGC+TGG + chr5_1:16108849-16108868 Msa0720170:five_prime_UTR 40.0%
!!! CTTTGGTTTTCTGCAGTGAT+GGG - chr5_1:16108972-16108991 None:intergenic 40.0%
!!! TCTTTGGTTTTCTGCAGTGA+TGG - chr5_1:16108973-16108992 None:intergenic 40.0%
ATCACTGCAGAAAACCAAAG+AGG + chr5_1:16108972-16108991 Msa0720170:CDS 40.0%
CAATAAGTAGACTGTGCAGA+AGG - chr5_1:16109002-16109021 None:intergenic 40.0%
CAGAAATCATGAGCTACCAA+TGG - chr5_1:16109030-16109049 None:intergenic 40.0%
ATACATCTCCTCTGCTATGT+GGG - chr5_1:16109139-16109158 None:intergenic 40.0%
TCTGCTTCTAGTAATGATCC+TGG + chr5_1:16109222-16109241 Msa0720170:CDS 40.0%
TCTAGTAATGATCCTGGTTC+TGG + chr5_1:16109228-16109247 Msa0720170:CDS 40.0%
TTGAACATCATGATGGCAGT+TGG + chr5_1:16109262-16109281 Msa0720170:CDS 40.0%
!! GAGGCTTATTGATTCTGAAG+AGG + chr5_1:16109284-16109303 Msa0720170:CDS 40.0%
AACAAAGACATCAATGTGGC+AGG - chr5_1:16109322-16109341 None:intergenic 40.0%
! ACAGAGCATAGCCATTGTTT+TGG + chr5_1:16109459-16109478 Msa0720170:intron 40.0%
ATCAAAGGCCTGCATATGAA+TGG - chr5_1:16109540-16109559 None:intergenic 40.0%
AAAATTGGAAGCAAAGGGGA+AGG + chr5_1:16109674-16109693 Msa0720170:CDS 40.0%
ACCAGAGTTCAACTCATGAA+GGG - chr5_1:16109774-16109793 None:intergenic 40.0%
AACCAGAGTTCAACTCATGA+AGG - chr5_1:16109775-16109794 None:intergenic 40.0%
AAAAGAAAGCTGCCTTGAGA+AGG + chr5_1:16109846-16109865 Msa0720170:CDS 40.0%
CATTTGACGGAAATGTTTGG+TGG - chr5_1:16110027-16110046 None:intergenic 40.0%
TTAAGCAATCTTGTCCTCAG+AGG - chr5_1:16110124-16110143 None:intergenic 40.0%
TCTGAGGACAAGATTGCTTA+AGG + chr5_1:16110123-16110142 Msa0720170:CDS 40.0%
!! TCTGTTCTCTCTGAAGCATT+CGG + chr5_1:16110301-16110320 Msa0720170:CDS 40.0%
! CTGAAGCATTCGGAACAATT+TGG + chr5_1:16110311-16110330 Msa0720170:CDS 40.0%
!! CAACTGATTTTAGGAAGCGT+TGG + chr5_1:16110335-16110354 Msa0720170:CDS 40.0%
!!! ACTGATTTTAGGAAGCGTTG+GGG + chr5_1:16110337-16110356 Msa0720170:CDS 40.0%
GTTGAGTAAATCATCATCCG+TGG + chr5_1:16110366-16110385 Msa0720170:CDS 40.0%
AACCTGTAAAAGTTCCTCCA+CGG - chr5_1:16110386-16110405 None:intergenic 40.0%
!! TATGCATGTCAGCATTGACA+TGG - chr5_1:16110490-16110509 None:intergenic 40.0%
CTCTACAACAGACGAATTGT+TGG + chr5_1:16110584-16110603 Msa0720170:CDS 40.0%
CTCAAGCTTTACGTAGTTGA+TGG - chr5_1:16110735-16110754 None:intergenic 40.0%
CAAGCCTAACAGAGAATTTG+AGG + chr5_1:16110932-16110951 Msa0720170:CDS 40.0%
TACTGGCCGTCTAAACATTA+AGG + chr5_1:16111100-16111119 Msa0720170:CDS 40.0%
CGGTGACCTTAATGTTTAGA+CGG - chr5_1:16111109-16111128 None:intergenic 40.0%
GAAATTAAAACAGCAGGGAC+AGG + chr5_1:16111142-16111161 Msa0720170:CDS 40.0%
! CTCTACGTTTTCTGACTGTT+CGG - chr5_1:16111199-16111218 None:intergenic 40.0%
!! AGCTGTTGAAGATTTGTTGC+TGG + chr5_1:16111226-16111245 Msa0720170:CDS 40.0%
!! GCTGTTGAAGATTTGTTGCT+GGG + chr5_1:16111227-16111246 Msa0720170:CDS 40.0%
CTATGACTCCTGTTCACATT+GGG + chr5_1:16111336-16111355 Msa0720170:intron 40.0%
TATGACTCCTGTTCACATTG+GGG + chr5_1:16111337-16111356 Msa0720170:intron 40.0%
GAGAATAGTAACTGCTGAAG+AGG + chr5_1:16111365-16111384 Msa0720170:intron 40.0%
AATAGTAACTGCTGAAGAGG+TGG + chr5_1:16111368-16111387 Msa0720170:intron 40.0%
! TGATGATAATGCTCAAGCAG+TGG + chr5_1:16111395-16111414 Msa0720170:CDS 40.0%
CAAGCAGTGGAATATGATCA+TGG + chr5_1:16111462-16111481 Msa0720170:CDS 40.0%
GATCATGGAAATTGGGAGAT+AGG + chr5_1:16111477-16111496 Msa0720170:CDS 40.0%
AATCCCTATCCCATCTGTTA+GGG - chr5_1:16111518-16111537 None:intergenic 40.0%
AAATCCCTATCCCATCTGTT+AGG - chr5_1:16111519-16111538 None:intergenic 40.0%
TAACAGATGGGATAGGGATT+TGG + chr5_1:16111518-16111537 Msa0720170:CDS 40.0%
TGGAATGAGTGATGAAGATG+TGG + chr5_1:16111542-16111561 Msa0720170:CDS 40.0%
GAGGAAGATGTTATGAGTGA+AGG + chr5_1:16111606-16111625 Msa0720170:CDS 40.0%
TGCAGTGGCAAAAATGCGAA+AGG + chr5_1:16107771-16107790 Msa0720170:five_prime_UTR 45.0%
AGAGCTTGCAGAGAAGATTC+AGG + chr5_1:16107899-16107918 Msa0720170:five_prime_UTR 45.0%
GCAACCGAAATTGATGCTTC+AGG - chr5_1:16108038-16108057 None:intergenic 45.0%
! TCGGTTGCTTTGACAAGAGA+TGG + chr5_1:16108050-16108069 Msa0720170:five_prime_UTR 45.0%
GGATCCTGAAGACGATGATT+TGG + chr5_1:16108265-16108284 Msa0720170:five_prime_UTR 45.0%
GATTTCCACAAGCAACTGGT+TGG - chr5_1:16108476-16108495 None:intergenic 45.0%
AAAGCAAGTCTGGAGAACCA+TGG + chr5_1:16108535-16108554 Msa0720170:five_prime_UTR 45.0%
AAGCAAGTCTGGAGAACCAT+GGG + chr5_1:16108536-16108555 Msa0720170:five_prime_UTR 45.0%
ACCATGGGTTCAAGGACTTA+CGG + chr5_1:16108551-16108570 Msa0720170:five_prime_UTR 45.0%
!! TCTTGTTGCATTGGTTGGTG+TGG + chr5_1:16108617-16108636 Msa0720170:five_prime_UTR 45.0%
! TTGGTTGGTGTGGCAAATGA+AGG + chr5_1:16108627-16108646 Msa0720170:five_prime_UTR 45.0%
! GTTGACGTGAACTGTTTCTC+AGG + chr5_1:16108734-16108753 Msa0720170:intron 45.0%
ACAAGACACATATCCTGCTG+TGG + chr5_1:16108887-16108906 Msa0720170:five_prime_UTR 45.0%
! ATCTGACTCTGAGAGTTGTC+CGG - chr5_1:16109066-16109085 None:intergenic 45.0%
CATACATCTCCTCTGCTATG+TGG - chr5_1:16109140-16109159 None:intergenic 45.0%
CAGAGGAGATGTATGTCTAC+AGG + chr5_1:16109145-16109164 Msa0720170:CDS 45.0%
AACATCATGATGGCAGTTGG+AGG + chr5_1:16109265-16109284 Msa0720170:CDS 45.0%
TTGACATCACTGGATTCACG+CGG + chr5_1:16109566-16109585 Msa0720170:CDS 45.0%
!! AACTCATGAAGGGTACTGCT+AGG - chr5_1:16109764-16109783 None:intergenic 45.0%
! ACCCTTCATGAGTTGAACTC+TGG + chr5_1:16109770-16109789 Msa0720170:CDS 45.0%
GGTAAAGAGATGCAAACCTC+AGG + chr5_1:16109940-16109959 Msa0720170:CDS 45.0%
AAAGAGATGCAAACCTCAGG+TGG + chr5_1:16109943-16109962 Msa0720170:CDS 45.0%
TGGAACATTCTCTTCCTCTG+AGG + chr5_1:16110107-16110126 Msa0720170:CDS 45.0%
TAAGGTCCTGCTGTCATGTA+TGG + chr5_1:16110141-16110160 Msa0720170:CDS 45.0%
! CATGTATGGAGGTGAGTTGA+TGG + chr5_1:16110155-16110174 Msa0720170:intron 45.0%
!! AGGTGAGTTGATGGCAACTT+TGG + chr5_1:16110164-16110183 Msa0720170:intron 45.0%
GAGTAAATCATCATCCGTGG+AGG + chr5_1:16110369-16110388 Msa0720170:CDS 45.0%
! ATCCGTGGAGGAACTTTTAC+AGG + chr5_1:16110381-16110400 Msa0720170:CDS 45.0%
CGTGTCCTCTGATCTTGAAT+CGG + chr5_1:16110640-16110659 Msa0720170:intron 45.0%
! AGCAACCGATTCAAGATCAG+AGG - chr5_1:16110648-16110667 None:intergenic 45.0%
AATCGGTTGCTCTACTTCCA+TGG + chr5_1:16110657-16110676 Msa0720170:intron 45.0%
ATCGGTTGCTCTACTTCCAT+GGG + chr5_1:16110658-16110677 Msa0720170:intron 45.0%
GTTGCTTCTTTCTCCTCAAC+AGG - chr5_1:16110767-16110786 None:intergenic 45.0%
GCTGCCTCAAATTCTCTGTT+AGG - chr5_1:16110939-16110958 None:intergenic 45.0%
CACGAAGTGGAAACAAACGT+GGG + chr5_1:16111012-16111031 Msa0720170:CDS 45.0%
GAGGAGCTAGTGATATTGGA+CGG + chr5_1:16111036-16111055 Msa0720170:CDS 45.0%
AGGAGCTAGTGATATTGGAC+GGG + chr5_1:16111037-16111056 Msa0720170:CDS 45.0%
TAGACGGCCAGTATCTTGTT+TGG - chr5_1:16111093-16111112 None:intergenic 45.0%
TAATGGTTCTCTTCCACCCT+TGG - chr5_1:16111276-16111295 None:intergenic 45.0%
CCAATGTGAACAGGAGTCAT+AGG - chr5_1:16111338-16111357 None:intergenic 45.0%
CCTATGACTCCTGTTCACAT+TGG + chr5_1:16111335-16111354 Msa0720170:intron 45.0%
CACTGCTTGAGCATCATCAT+CGG - chr5_1:16111424-16111443 None:intergenic 45.0%
CACTGCTTGAGCATCATCAT+CGG - chr5_1:16111424-16111443 None:intergenic 45.0%
ATCCCTATCCCATCTGTTAG+GGG - chr5_1:16111517-16111536 None:intergenic 45.0%
! AGATGGGATAGGGATTTGGT+TGG + chr5_1:16111522-16111541 Msa0720170:CDS 45.0%
CAATGAAGAGGAAGATTCCG+AGG + chr5_1:16111587-16111606 Msa0720170:CDS 45.0%
GTTATGAGTGAAGGTTCGGA+TGG + chr5_1:16111615-16111634 Msa0720170:CDS 45.0%
AACCTGTGTCTCGATCTTCG+AGG - chr5_1:16107807-16107826 None:intergenic 50.0%
TCGAGACACAGGTTAACTCC+AGG + chr5_1:16107813-16107832 Msa0720170:five_prime_UTR 50.0%
TGATGCTTCAGGTGTCTTGC+TGG - chr5_1:16108027-16108046 None:intergenic 50.0%
! AGAATGGACTCAGCATCTGC+AGG - chr5_1:16108137-16108156 None:intergenic 50.0%
GCATCCTTGGAACCATTCTC+AGG - chr5_1:16108425-16108444 None:intergenic 50.0%
AGAGGGACAATTGGCATCCT+TGG - chr5_1:16108438-16108457 None:intergenic 50.0%
GGTGACAGAAGAGGGACAAT+TGG - chr5_1:16108447-16108466 None:intergenic 50.0%
GGTTGTCTGGTGACAGAAGA+GGG - chr5_1:16108455-16108474 None:intergenic 50.0%
TGGTTGTCTGGTGACAGAAG+AGG - chr5_1:16108456-16108475 None:intergenic 50.0%
! CAAGCAACTGGTTGGTTGTC+TGG - chr5_1:16108468-16108487 None:intergenic 50.0%
GACAACCAACCAGTTGCTTG+TGG + chr5_1:16108468-16108487 Msa0720170:five_prime_UTR 50.0%
TCTGGAGAACCATGGGTTCA+AGG + chr5_1:16108543-16108562 Msa0720170:five_prime_UTR 50.0%
TCCGTAAGTCCTTGAACCCA+TGG - chr5_1:16108555-16108574 None:intergenic 50.0%
TGGGTTCAAGGACTTACGGA+AGG + chr5_1:16108555-16108574 Msa0720170:five_prime_UTR 50.0%
AGACACATATCCTGCTGTGG+AGG + chr5_1:16108890-16108909 Msa0720170:five_prime_UTR 50.0%
GACACATATCCTGCTGTGGA+GGG + chr5_1:16108891-16108910 Msa0720170:five_prime_UTR 50.0%
! CTTTGATTTCCCTCCACAGC+AGG - chr5_1:16108903-16108922 None:intergenic 50.0%
GTGCAGAAGGAGTTCCTCTT+TGG - chr5_1:16108989-16109008 None:intergenic 50.0%
GTAGCTCATGATTTCTGCCC+AGG + chr5_1:16109033-16109052 Msa0720170:CDS 50.0%
!! GTAATGATCCTGGTTCTGGC+AGG + chr5_1:16109232-16109251 Msa0720170:CDS 50.0%
ACAAAGATCCTGCCAGAACC+AGG - chr5_1:16109243-16109262 None:intergenic 50.0%
TGACATCACTGGATTCACGC+GGG + chr5_1:16109567-16109586 Msa0720170:CDS 50.0%
TAGGACTGCCAGATCCTGAA+TGG - chr5_1:16109745-16109764 None:intergenic 50.0%
! TTGGTGGCAGGAATTGCAGT+GGG - chr5_1:16110011-16110030 None:intergenic 50.0%
! TTTGGTGGCAGGAATTGCAG+TGG - chr5_1:16110012-16110031 None:intergenic 50.0%
! TGACGGAAATGTTTGGTGGC+AGG - chr5_1:16110023-16110042 None:intergenic 50.0%
TCACCTCCATACATGACAGC+AGG - chr5_1:16110150-16110169 None:intergenic 50.0%
GACAATGCTGCAGTGGTTAG+AGG - chr5_1:16110686-16110705 None:intergenic 50.0%
TGAGAGACTTGAGCCTGTTG+AGG + chr5_1:16110751-16110770 Msa0720170:CDS 50.0%
GCAGCAGCTCTTGATCATGA+TGG + chr5_1:16110954-16110973 Msa0720170:CDS 50.0%
GCACGAAGTGGAAACAAACG+TGG + chr5_1:16111011-16111030 Msa0720170:CDS 50.0%
GGAGCTAGTGATATTGGACG+GGG + chr5_1:16111038-16111057 Msa0720170:CDS 50.0%
GACAGGGAACACAAGGACAA+GGG + chr5_1:16111159-16111178 Msa0720170:CDS 50.0%
ACAGGGAACACAAGGACAAG+GGG + chr5_1:16111160-16111179 Msa0720170:CDS 50.0%
! AACTATGACTGGCTGCTGCA+TGG - chr5_1:16111253-16111272 None:intergenic 50.0%
AGCAGCCAGTCATAGTTCCA+AGG + chr5_1:16111256-16111275 Msa0720170:CDS 50.0%
GCAGCCAGTCATAGTTCCAA+GGG + chr5_1:16111257-16111276 Msa0720170:CDS 50.0%
TCCACCCTTGGAACTATGAC+TGG - chr5_1:16111264-16111283 None:intergenic 50.0%
TCAGCAACCCCAATGTGAAC+AGG - chr5_1:16111347-16111366 None:intergenic 50.0%
! CGATGATGATGCTCAAGCAG+TGG + chr5_1:16111422-16111441 Msa0720170:CDS 50.0%
! CGATGATGATGCTCAAGCAG+TGG + chr5_1:16111422-16111441 Msa0720170:CDS 50.0%
TCTCCCCTAACAGATGGGAT+AGG + chr5_1:16111511-16111530 Msa0720170:CDS 50.0%
CTCCCCTAACAGATGGGATA+GGG + chr5_1:16111512-16111531 Msa0720170:CDS 50.0%
TTCAGCAGCCGTAACTGCAG+TGG + chr5_1:16107756-16107775 Msa0720170:five_prime_UTR 55.0%
CTCCTCGAAGATCGAGACAC+AGG + chr5_1:16107802-16107821 Msa0720170:five_prime_UTR 55.0%
!! AACCTTTGGGGCCCTCAAGA+GGG - chr5_1:16107869-16107888 None:intergenic 55.0%
!! AAACCTTTGGGGCCCTCAAG+AGG - chr5_1:16107870-16107889 None:intergenic 55.0%
! CAGGTGTCTTGCTGGTTGTC+AGG - chr5_1:16108019-16108038 None:intergenic 55.0%
CATGATTTCTGCCCAGGCAC+AGG + chr5_1:16109039-16109058 Msa0720170:CDS 55.0%
GTCTACAGGTCTCTACCCCT+TGG + chr5_1:16109159-16109178 Msa0720170:CDS 55.0%
ACCGCAGGCGTAACCGATAT+TGG + chr5_1:16109187-16109206 Msa0720170:CDS 55.0%
! GCCAATATCGGTTACGCCTG+CGG - chr5_1:16109191-16109210 None:intergenic 55.0%
ACTGGATTCACGCGGGATCA+GGG + chr5_1:16109574-16109593 Msa0720170:CDS 55.0%
CCAGATCCTGAATGGCACTC+GGG - chr5_1:16109737-16109756 None:intergenic 55.0%
GCCAGATCCTGAATGGCACT+CGG - chr5_1:16109738-16109757 None:intergenic 55.0%
GGTCCTGCTGTCATGTATGG+AGG + chr5_1:16110144-16110163 Msa0720170:CDS 55.0%
GCAGTGGTTAGAGGAACCCA+TGG - chr5_1:16110677-16110696 None:intergenic 55.0%
CCTGAGAGACAATGCTGCAG+TGG - chr5_1:16110693-16110712 None:intergenic 55.0%
CCACTGCAGCATTGTCTCTC+AGG + chr5_1:16110690-16110709 Msa0720170:intron 55.0%
AGTGGAAACAAACGTGGGCG+AGG + chr5_1:16111017-16111036 Msa0720170:CDS 55.0%
GGGCGAGGAGCTAGTGATAT+TGG + chr5_1:16111032-16111051 Msa0720170:CDS 55.0%
GGACAGGGAACACAAGGACA+AGG + chr5_1:16111158-16111177 Msa0720170:CDS 55.0%
CAGGGAACACAAGGACAAGG+GGG + chr5_1:16111161-16111180 Msa0720170:CDS 55.0%
GCCAGTCATAGTTCCAAGGG+TGG + chr5_1:16111260-16111279 Msa0720170:intron 55.0%
ACGGTGTCTCCCCTAACAGA+TGG + chr5_1:16111505-16111524 Msa0720170:CDS 55.0%
CGGTGTCTCCCCTAACAGAT+GGG + chr5_1:16111506-16111525 Msa0720170:CDS 55.0%
TTTCTGCCCAGGCACAGGTC+CGG + chr5_1:16109044-16109063 Msa0720170:CDS 60.0%
AGTTGTCCGGACCTGTGCCT+GGG - chr5_1:16109053-16109072 None:intergenic 60.0%
CACTGGATTCACGCGGGATC+AGG + chr5_1:16109573-16109592 Msa0720170:CDS 60.0%
! CCCGAGTGCCATTCAGGATC+TGG + chr5_1:16109734-16109753 Msa0720170:CDS 60.0%
CAGCAGGGACAGGGAACACA+AGG + chr5_1:16111152-16111171 Msa0720170:CDS 60.0%
GGAACACAAGGACAAGGGGG+TGG + chr5_1:16111164-16111183 Msa0720170:CDS 60.0%
CTCCCTCTTGAGGGCCCCAA+AGG + chr5_1:16107864-16107883 Msa0720170:five_prime_UTR 65.0%
GAGTTGTCCGGACCTGTGCC+TGG - chr5_1:16109054-16109073 None:intergenic 65.0%
CAGGTCTCTACCCCTTGGCC+AGG + chr5_1:16109164-16109183 Msa0720170:CDS 65.0%
ATCGGTTACGCCTGCGGTCC+TGG - chr5_1:16109185-16109204 None:intergenic 65.0%
CCTGAATGGCACTCGGGCAC+TGG - chr5_1:16109731-16109750 None:intergenic 65.0%
! CCAGTGCCCGAGTGCCATTC+AGG + chr5_1:16109728-16109747 Msa0720170:CDS 65.0%
CAAGGACAAGGGGGTGGACG+AGG + chr5_1:16111170-16111189 Msa0720170:CDS 65.0%
TACCCCTTGGCCAGGACCGC+AGG + chr5_1:16109172-16109191 Msa0720170:CDS 70.0%
ACGCCTGCGGTCCTGGCCAA+GGG - chr5_1:16109178-16109197 None:intergenic 70.0%
TACGCCTGCGGTCCTGGCCA+AGG - chr5_1:16109179-16109198 None:intergenic 70.0%
CGCCTGCGGTCCTGGCCAAG+GGG - chr5_1:16109177-16109196 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 16107715 16111704 16107715 ID=Msa0720170;Name=Msa0720170
chr5_1 mRNA 16107715 16111704 16107715 ID=Msa0720170-mRNA-1;Parent=Msa0720170;Name=Msa0720170-mRNA-1;_AED=0.13;_eAED=0.13;_QI=1084|0.62|0.55|1|1|0.77|9|0|648
chr5_1 exon 16107715 16107920 16107715 ID=Msa0720170-mRNA-1:exon:6157;Parent=Msa0720170-mRNA-1
chr5_1 exon 16107999 16108674 16107999 ID=Msa0720170-mRNA-1:exon:6158;Parent=Msa0720170-mRNA-1
chr5_1 exon 16108756 16109305 16108756 ID=Msa0720170-mRNA-1:exon:6159;Parent=Msa0720170-mRNA-1
chr5_1 exon 16109551 16110165 16109551 ID=Msa0720170-mRNA-1:exon:6160;Parent=Msa0720170-mRNA-1
chr5_1 exon 16110298 16110402 16110298 ID=Msa0720170-mRNA-1:exon:6161;Parent=Msa0720170-mRNA-1
chr5_1 exon 16110531 16110619 16110531 ID=Msa0720170-mRNA-1:exon:6162;Parent=Msa0720170-mRNA-1
chr5_1 exon 16110712 16110796 16110712 ID=Msa0720170-mRNA-1:exon:6163;Parent=Msa0720170-mRNA-1
chr5_1 exon 16110906 16111278 16110906 ID=Msa0720170-mRNA-1:exon:6164;Parent=Msa0720170-mRNA-1
chr5_1 exon 16111373 16111704 16111373 ID=Msa0720170-mRNA-1:exon:6165;Parent=Msa0720170-mRNA-1
chr5_1 five_prime_UTR 16107715 16107920 16107715 ID=Msa0720170-mRNA-1:five_prime_utr;Parent=Msa0720170-mRNA-1
chr5_1 five_prime_UTR 16107999 16108674 16107999 ID=Msa0720170-mRNA-1:five_prime_utr;Parent=Msa0720170-mRNA-1
chr5_1 five_prime_UTR 16108756 16108957 16108756 ID=Msa0720170-mRNA-1:five_prime_utr;Parent=Msa0720170-mRNA-1
chr5_1 CDS 16108958 16109305 16108958 ID=Msa0720170-mRNA-1:cds;Parent=Msa0720170-mRNA-1
chr5_1 CDS 16109551 16110165 16109551 ID=Msa0720170-mRNA-1:cds;Parent=Msa0720170-mRNA-1
chr5_1 CDS 16110298 16110402 16110298 ID=Msa0720170-mRNA-1:cds;Parent=Msa0720170-mRNA-1
chr5_1 CDS 16110531 16110619 16110531 ID=Msa0720170-mRNA-1:cds;Parent=Msa0720170-mRNA-1
chr5_1 CDS 16110712 16110796 16110712 ID=Msa0720170-mRNA-1:cds;Parent=Msa0720170-mRNA-1
chr5_1 CDS 16110906 16111278 16110906 ID=Msa0720170-mRNA-1:cds;Parent=Msa0720170-mRNA-1
chr5_1 CDS 16111373 16111704 16111373 ID=Msa0720170-mRNA-1:cds;Parent=Msa0720170-mRNA-1
Gene Sequence

>Msa0720170

ATGAAGCATCACCCATCACTGCAGAAAACCAAAGAGGAACTCCTTCTGCACAGTCTACTTATTGAAATGCCATTGGTAGCTCATGATTTCTGCCCAGGCACAGGTCCGGACAACTCTCAGAGTCAGATGCATGCACAATATTCAAAAAGGTCACGATCACAGTTAAAATCTTATATTTCCCACATAGCAGAGGAGATGTATGTCTACAGGTCTCTACCCCTTGGCCAGGACCGCAGGCGTAACCGATATTGGCAGTTTGTTGCATCTGCTTCTAGTAATGATCCTGGTTCTGGCAGGATCTTTGTTGAACATCATGATGGCAGTTGGAGGCTTATTGATTCTGAAGAGGCCTTTGATATTCTTTTGACATCACTGGATTCACGCGGGATCAGGGAGTCTCATTTGCGCTTAATGTTGCAAAAGATTGAGAAATCCTTTAGAGAAAATGTTCGAAAAAATACACAATATACAAAAATTGGAAGCAAAGGGGAAGGTTCTATGAAAACTGAAGCTGATGAAGCATATCCAGTGCCCGAGTGCCATTCAGGATCTGGCAGTCCTAGCAGTACCCTTCATGAGTTGAACTCTGGTTCATCTGAAACTTCATCATCCTTCAAAATTGAGCTTGGCAAAAGTGACAATGAAAAGAAAGCTGCCTTGAGAAGGTATCAAGATTTTCAGAAGTGGATGTGGAAAGAATGCTATAACTCATCAATTTTATGTGCAATAAAATTTGGGGTAAAGAGATGCAAACCTCAGGTGGATATTTGTGAAATCTGTCTTGATCCTTATTTTATGGAAGACTCCCACTGCAATTCCTGCCACCAAACATTTCCGTCAAATGATGAATTTAATTTTTCTAAACACACATTTCAATGTGTTGGCAATTTATCCAAAGATATTATGGAACATTCTCTTCCTCTGAGGACAAGATTGCTTAAGGTCCTGCTGTCATGTATGGAGGCATCTGTTCTCTCTGAAGCATTCGGAACAATTTGGACAACTGATTTTAGGAAGCGTTGGGGTGTGAAGTTGAGTAAATCATCATCCGTGGAGGAACTTTTACAGATGTTGACCTTATTTGAAAAAGTGCTACGGCGTGATTTTCTATCATCAAACTTCTCTACAACAGACGAATTGTTGGGATTAAAAGCAGTGCTTTTTGAGTTCGATTCATCCATCAACTACGTAAAGCTTGAGAGACTTGAGCCTGTTGAGGAGAAAGAAGCAACAGAATACATTAGGCTTCCATCAAGATATACTCCCTTCAAGCCTAACAGAGAATTTGAGGCAGCAGCTCTTGATCATGATGGATTCACCAAAGTTAAACCTTCTGCTAATAAAATTGCACGAAGTGGAAACAAACGTGGGCGAGGAGCTAGTGATATTGGACGGGGTAAAAAGCTGTCTAAAAGAATGTACAATTCCAAACAAGATACTGGCCGTCTAAACATTAAGGTCACCGAGAATCTAAGTCAGAAATTAAAACAGCAGGGACAGGGAACACAAGGACAAGGGGGTGGACGAGGTCGCCGAACAGTCAGAAAACGTAGAGTAAAAAGAGCTGTTGAAGATTTGTTGCTGGGCCATGCAGCAGCCAGTCATAGTTCCAAGGTAACTGCTGAAGAGGTGGAGTCTGATGATAATGCTCAAGCAGTGGAATCCGATGATGATGCTCAAGCAGTGGAATCCGATGATGATGCTCAAGCAGTGGAATATGATCATGGAAATTGGGAGATAGGTTACAACGGTGTCTCCCCTAACAGATGGGATAGGGATTTGGTTGGAATGAGTGATGAAGATGTGGACAATTTTGAAGATGAAGAAGACAATGAAGAGGAAGATTCCGAGGAAGATGTTATGAGTGAAGGTTCGGATGGAATGCTGAATAGAGTAGTGAATGTAGGGGGCTCTGAAGATGTTATGAGTGAAGATTCTTCTGATTAG
Protein sequence

>Msa0720170

MKHHPSLQKTKEELLLHSLLIEMPLVAHDFCPGTGPDNSQSQMHAQYSKRSRSQLKSYISHIAEEMYVYRSLPLGQDRRRNRYWQFVASASSNDPGSGRIFVEHHDGSWRLIDSEEAFDILLTSLDSRGIRESHLRLMLQKIEKSFRENVRKNTQYTKIGSKGEGSMKTEADEAYPVPECHSGSGSPSSTLHELNSGSSETSSSFKIELGKSDNEKKAALRRYQDFQKWMWKECYNSSILCAIKFGVKRCKPQVDICEICLDPYFMEDSHCNSCHQTFPSNDEFNFSKHTFQCVGNLSKDIMEHSLPLRTRLLKVLLSCMEASVLSEAFGTIWTTDFRKRWGVKLSKSSSVEELLQMLTLFEKVLRRDFLSSNFSTTDELLGLKAVLFEFDSSINYVKLERLEPVEEKEATEYIRLPSRYTPFKPNREFEAAALDHDGFTKVKPSANKIARSGNKRGRGASDIGRGKKLSKRMYNSKQDTGRLNIKVTENLSQKLKQQGQGTQGQGGGRGRRTVRKRRVKRAVEDLLLGHAAASHSSKVTAEEVESDDNAQAVESDDDAQAVESDDDAQAVEYDHGNWEIGYNGVSPNRWDRDLVGMSDEDVDNFEDEEDNEEEDSEEDVMSEGSDGMLNRVVNVGGSEDVMSEDSSD*