AlfalfaGEDB | Msa0720560

M. sativa cultivar zhongmu-4 / Msa0720560

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0720560	GAU37415.1	32.441	299	99	2	7	202	657	955	1.69e-39	159

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0720560	A0A2Z6NNG9	32.441	299	99	2	7	202	657	955	8.07e-40	159

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0720560	Msa1346370	0.803793	2.941645e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0720560	MtrunA17_Chr8g0359381	49.600	125	52	5	1	119	602	721	3.12e-28	115
Msa0720560	MtrunA17_Chr8g0359381	33.019	106	71	0	97	202	866	971	6.58e-11	63.5
Msa0720560	MtrunA17_Chr5g0431011	54.717	106	41	4	1	100	254	358	9.16e-28	113
Msa0720560	MtrunA17_Chr4g0002791	48.000	125	54	6	1	119	192	311	8.02e-26	107
Msa0720560	MtrunA17_Chr3g0131371	47.115	104	51	2	2	102	138	240	1.01e-24	103
Msa0720560	MtrunA17_Chr2g0296731	55.172	87	39	0	1	87	165	251	1.24e-24	103
Msa0720560	MtrunA17_Chr2g0296731	46.269	67	36	0	128	194	429	495	3.66e-12	67.0
Msa0720560	MtrunA17_Chr8g0363541	51.163	86	39	1	13	95	156	241	3.51e-24	100
Msa0720560	MtrunA17_Chr2g0314641	53.333	75	32	1	13	87	1	72	1.23e-21	89.7
Msa0720560	MtrunA17_Chr4g0020731	45.714	105	39	3	2	106	449	535	3.94e-20	91.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 89 sgRNAs with CRISPR-Local

Find 368 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCGTGAAGGAATTAG+TGG	0.513457	5_1:-16588758	Msa0720560:CDS
AAAGTATACTCAGTAGCACA+AGG	0.696490	5_1:+16587761	None:intergenic
AAAGTTAAAATTTCTACCTT+AGG	0.356755	5_1:-16587930	Msa0720560:CDS
AAGGAATTAGTGGTGAGGGA+GGG	0.539781	5_1:-16588748	Msa0720560:CDS
AAGGTATAACATCTTAATGG+AGG	0.553875	5_1:-16585043	Msa0720560:CDS
AAGGTGTTGTGCTCTCGTTA+CGG	0.540065	5_1:-16588845	Msa0720560:CDS
ACAAATCTGCAGGTGATCTG+TGG	0.616006	5_1:+16584457	None:intergenic
ACTTAGTTCGCTTACTCTTC+AGG	0.297019	5_1:-16588077	Msa0720560:intron
AGCCCTTACTATCAAAGCAA+AGG	0.614993	5_1:-16585093	Msa0720560:CDS
AGCTGGCCTGAAAACGCAGC+TGG	0.481170	5_1:+16586001	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGATATTGAGACAGATATT+TGG	-	chr5_1:16584797-16584816	Msa0720560:intron	30.0%
ACATAAAATTGTTCCTCTGA+AGG	-	chr5_1:16584924-16584943	Msa0720560:intron	30.0%
ATTGTCTACAAAGGATAACT+TGG	-	chr5_1:16584981-16585000	Msa0720560:intron	30.0%
TTCCATTTGGTTATCATATG+TGG	-	chr5_1:16585373-16585392	Msa0720560:intron	30.0%
GCTATTAAAACAAGTCACTT+TGG	-	chr5_1:16585797-16585816	Msa0720560:intron	30.0%
ACAATATTCTTGTATCTCAC+CGG	+	chr5_1:16585823-16585842	None:intergenic	30.0%
TACTTCACAAATCATGTTCA+TGG	-	chr5_1:16586204-16586223	Msa0720560:intron	30.0%
ATTTCTCATGTTATGTAGGA+GGG	+	chr5_1:16586256-16586275	None:intergenic	30.0%
TGACATTTCTCATGTTATGT+AGG	+	chr5_1:16586260-16586279	None:intergenic	30.0%
CATAACATGAGAAATGTCAA+AGG	-	chr5_1:16586261-16586280	Msa0720560:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	16584298	16588978	16584298	ID=Msa0720560;Name=Msa0720560
chr5_1	mRNA	16584298	16588978	16584298	ID=Msa0720560-mRNA-1;Parent=Msa0720560;Name=Msa0720560-mRNA-1;_AED=0.69;_eAED=0.76;_QI=0\|0\|0.14\|0.42\|1\|1\|7\|155\|319
chr5_1	exon	16584298	16584598	16584298	ID=Msa0720560-mRNA-1:exon:6309;Parent=Msa0720560-mRNA-1
chr5_1	exon	16585043	16585120	16585043	ID=Msa0720560-mRNA-1:exon:6308;Parent=Msa0720560-mRNA-1
chr5_1	exon	16585960	16586059	16585960	ID=Msa0720560-mRNA-1:exon:6307;Parent=Msa0720560-mRNA-1
chr5_1	exon	16587751	16587822	16587751	ID=Msa0720560-mRNA-1:exon:6306;Parent=Msa0720560-mRNA-1
chr5_1	exon	16587911	16588093	16587911	ID=Msa0720560-mRNA-1:exon:6305;Parent=Msa0720560-mRNA-1
chr5_1	exon	16588358	16588477	16588358	ID=Msa0720560-mRNA-1:exon:6304;Parent=Msa0720560-mRNA-1
chr5_1	exon	16588718	16588978	16588718	ID=Msa0720560-mRNA-1:exon:6303;Parent=Msa0720560-mRNA-1
chr5_1	CDS	16588718	16588978	16588718	ID=Msa0720560-mRNA-1:cds;Parent=Msa0720560-mRNA-1
chr5_1	CDS	16588358	16588477	16588358	ID=Msa0720560-mRNA-1:cds;Parent=Msa0720560-mRNA-1
chr5_1	CDS	16587911	16588093	16587911	ID=Msa0720560-mRNA-1:cds;Parent=Msa0720560-mRNA-1
chr5_1	CDS	16587751	16587822	16587751	ID=Msa0720560-mRNA-1:cds;Parent=Msa0720560-mRNA-1
chr5_1	CDS	16585960	16586059	16585960	ID=Msa0720560-mRNA-1:cds;Parent=Msa0720560-mRNA-1
chr5_1	CDS	16585043	16585120	16585043	ID=Msa0720560-mRNA-1:cds;Parent=Msa0720560-mRNA-1
chr5_1	CDS	16584453	16584598	16584453	ID=Msa0720560-mRNA-1:cds;Parent=Msa0720560-mRNA-1
chr5_1	three_prime_UTR	16584298	16584452	16584298	ID=Msa0720560-mRNA-1:three_prime_utr;Parent=Msa0720560-mRNA-1

Gene Sequence

>Msa0720560

ATGAGCAAGGAGTTAGGTGGTTTGAGGGTGCGACGGTTGAAGGAATTTAATATTGCTATTTTAGGTAAATGGTGTTGGAGGTGTCTAGTGGATAGGGAGGGGTTGTGGTTTAAGGTGTTGTGCTCTCGTTACGGTGAGGAGAGGGGGCACATTAAAGAAGGGGCGAGGGATGGTTCTGCCTGGTGGAGGGAGATAGTGAAAATCCGTGAAGGAATTAGTGGTGAGGGAGGGAGGTGGTTTGAGGAAAGTATTCAAAGGAAGGATATTGAGACAGATATTTGGCAGTGGTGTCCTACTGTGGGTGATGGATATACCGTTTGTGGTGTGTATCAAATGCTTATGAGGCAGGAGATGCATAGCTACAATGAGGCGTTGGGGGCTTTCGCTTACTCTTCAGGTGGCATTAAATCTCGTCGTTCTTTTTTGCAAATTGTATCGCTTTGCACCGTTTGGGTGTTATGGAGTGAAAGGAATAACATGCTATTCTCCAACAAGACTAAATCACTTATGCAGCTTCTAGATAAAGTTAAAATTTCTACCTTAGGATGGCTAAAAGCTAAAATTGTTACGTTGTTGTACTCTTGTTATGTTCTCCTTTGCACACCTTGTGCTACTGAGTATACTTTGTTTCCTGTGAAGGTGCAGGCCAGCTGCGTTTTCAGAAGGTGCAGGCCAGCTGCGTTTTCAGGCCAGCTGGGAAAAAAACACAGTGTGTCAGGTTTTCTGCACTTCTCTGTTGGAAGCCCTTACTATCAAAGCAAAGGACAGATCTTCTCTAAATTGACAAGTTTAAGGTATAACATCTTAATGGAGGTGATAATGTGTACTTGGACAACAGCAAGCAAGCATGTAAACAAGTCCTCATCAGACACTTTGACAACTCCTTCATCCCACAATGTCTCAAAGTGTTTGGATAGAGTCAAGCAAGCATGTCCACAGATCACCTGCAGATTTGTGTAG

Protein sequence

>Msa0720560

MSKELGGLRVRRLKEFNIAILGKWCWRCLVDREGLWFKVLCSRYGEERGHIKEGARDGSAWWREIVKIREGISGEGGRWFEESIQRKDIETDIWQWCPTVGDGYTVCGVYQMLMRQEMHSYNEALGAFAYSSGGIKSRRSFLQIVSLCTVWVLWSERNNMLFSNKTKSLMQLLDKVKISTLGWLKAKIVTLLYSCYVLLCTPCATEYTLFPVKVQASCVFRRCRPAAFSGQLGKKHSVSGFLHFSVGSPYYQSKGQIFSKLTSLRYNILMEVIMCTWTTASKHVNKSSSDTLTTPSSHNVSKCLDRVKQACPQITCRFV*