AlfalfaGEDB | Msa0722680

M. sativa cultivar zhongmu-4 / Msa0722680

KEGG: K14489
GO: GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0722680	AES97003.2	75.862	58	13	1	14	71	3	59	5.03e-17	83.2

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0722680	G7JWS8	75.862	58	13	1	14	71	3	59	2.40e-17	83.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0110270	Msa0722680	0.801844	7.434512e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 18 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTAACATGAAGCTAGTTCT+AGG	0.266641	5_1:-20083389	Msa0722680:CDS
GAACTAGCTTCATGTTAAAC+TGG	0.276068	5_1:+20083393	None:intergenic
TCATGTTAAACTGGGCTACT+AGG	0.394956	5_1:+20083402	None:intergenic
TACATGACGGTGCCAGTCCT+TGG	0.459883	5_1:+20083213	None:intergenic
TCAATGCGAAACACGACACT+TGG	0.475064	5_1:-20083362	Msa0722680:CDS
CATGTGAGGCTTTAGCATAC+AGG	0.483273	5_1:-20083277	Msa0722680:CDS
ATCCATTCCACACCAAGGAC+TGG	0.510778	5_1:-20083225	Msa0722680:CDS
TGCCAGTCCTTGGTGTGGAA+TGG	0.527045	5_1:+20083223	None:intergenic
GACATGTAGCAAAGAGGGAA+GGG	0.580985	5_1:-20083336	Msa0722680:CDS
GGATTGACATGTAGCAAAGA+GGG	0.591889	5_1:-20083341	Msa0722680:CDS
AACTAGCTTCATGTTAAACT+GGG	0.597597	5_1:+20083394	None:intergenic
TGACATGTAGCAAAGAGGGA+AGG	0.604720	5_1:-20083337	Msa0722680:CDS
TGGATTGACATGTAGCAAAG+AGG	0.639480	5_1:-20083342	Msa0722680:CDS
GACGGTGCCAGTCCTTGGTG+TGG	0.656751	5_1:+20083218	None:intergenic
ACACTCTAGTTATTACATGA+CGG	0.661666	5_1:+20083200	None:intergenic
GCTAAATCCATTCCACACCA+AGG	0.666931	5_1:-20083230	Msa0722680:CDS
GAGGCTTTAGCATACAGGAG+AGG	0.685073	5_1:-20083272	Msa0722680:CDS
ACTGAATCATGATACATGTG+AGG	0.687659	5_1:-20083291	Msa0722680:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGAGATGTTAATTGATTGT+TGG	-	chr5_1:20083356-20083375	Msa0722680:CDS	25.0%
!	AGCAATGAAACAAAGAAAAT+TGG	+	chr5_1:20083388-20083407	None:intergenic	25.0%
!!!	TTTTCTTTGTTTCATTGCTT+TGG	-	chr5_1:20083389-20083408	Msa0722680:CDS	25.0%
!	GATTAAAGCCATATTTGATA+AGG	-	chr5_1:20083414-20083433	Msa0722680:CDS	25.0%
	CAGTAGTACCTTATCAAATA+TGG	+	chr5_1:20083425-20083444	None:intergenic	30.0%
!!!	AGAGCATTGCAATTTTACTT+TGG	-	chr5_1:20083449-20083468	Msa0722680:CDS	30.0%
!	AACTAGCTTCATGTTAAACT+GGG	+	chr5_1:20083496-20083515	None:intergenic	30.0%
!	TTTAACATGAAGCTAGTTCT+AGG	-	chr5_1:20083498-20083517	Msa0722680:intron	30.0%
!	TCAGCATTTTCTAAGCTTAG+TGG	-	chr5_1:20083208-20083227	Msa0722680:CDS	35.0%
!	TTTTCTAAGCTTAGTGGTAG+TGG	-	chr5_1:20083214-20083233	Msa0722680:CDS	35.0%
	CATCAAATCTATCTGATTGC+TGG	-	chr5_1:20083316-20083335	Msa0722680:CDS	35.0%
!	GAACTAGCTTCATGTTAAAC+TGG	+	chr5_1:20083497-20083516	None:intergenic	35.0%
	ACTGAATCATGATACATGTG+AGG	-	chr5_1:20083596-20083615	Msa0722680:intron	35.0%
!	GGAGAAATCTGACTTGAAAG+AGG	-	chr5_1:20083235-20083254	Msa0722680:CDS	40.0%
	TCATGTTAAACTGGGCTACT+AGG	+	chr5_1:20083488-20083507	None:intergenic	40.0%
	TGGATTGACATGTAGCAAAG+AGG	-	chr5_1:20083545-20083564	Msa0722680:intron	40.0%
	GGATTGACATGTAGCAAAGA+GGG	-	chr5_1:20083546-20083565	Msa0722680:intron	40.0%
	TCAATGCGAAACACGACACT+TGG	-	chr5_1:20083525-20083544	Msa0722680:intron	45.0%
	TGACATGTAGCAAAGAGGGA+AGG	-	chr5_1:20083550-20083569	Msa0722680:intron	45.0%
	GACATGTAGCAAAGAGGGAA+GGG	-	chr5_1:20083551-20083570	Msa0722680:intron	45.0%
!	CATGTGAGGCTTTAGCATAC+AGG	-	chr5_1:20083610-20083629	Msa0722680:intron	45.0%
	GCTAAATCCATTCCACACCA+AGG	-	chr5_1:20083657-20083676	Msa0722680:intron	45.0%
!	GAGGCTTTAGCATACAGGAG+AGG	-	chr5_1:20083615-20083634	Msa0722680:intron	50.0%
	ATCCATTCCACACCAAGGAC+TGG	-	chr5_1:20083662-20083681	Msa0722680:intron	50.0%
!	TGCCAGTCCTTGGTGTGGAA+TGG	+	chr5_1:20083667-20083686	None:intergenic	55.0%
!	TACATGACGGTGCCAGTCCT+TGG	+	chr5_1:20083677-20083696	None:intergenic	55.0%
!	GACGGTGCCAGTCCTTGGTG+TGG	+	chr5_1:20083672-20083691	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	20083205	20083704	20083205	ID=Msa0722680;Name=Msa0722680
chr5_1	mRNA	20083205	20083704	20083205	ID=Msa0722680-mRNA-1;Parent=Msa0722680;Name=Msa0722680-mRNA-1;_AED=0.53;_eAED=0.66;_QI=0\|0\|0\|0.5\|0\|0\|2\|0\|97
chr5_1	exon	20083680	20083704	20083680	ID=Msa0722680-mRNA-1:exon:7606;Parent=Msa0722680-mRNA-1
chr5_1	exon	20083205	20083473	20083205	ID=Msa0722680-mRNA-1:exon:7605;Parent=Msa0722680-mRNA-1
chr5_1	CDS	20083680	20083704	20083680	ID=Msa0722680-mRNA-1:cds;Parent=Msa0722680-mRNA-1
chr5_1	CDS	20083205	20083473	20083205	ID=Msa0722680-mRNA-1:cds;Parent=Msa0722680-mRNA-1

Gene Sequence	>Msa0722680 ATGTCAGCATTTTCTAAGCTTAGTGGTACTACTGTACTAGAGCATTGCAATTTTACTTTGGCTGTAGCTGTAATCCTAGTAGCCCAGTTTAACATGAAGCTAGTTCTAGGAATTTCAATGCGAAACACGACACTTGGATTGACATGTAGCAAAGAGGGAAGGGTATTAGTTTCTTGTCATTGCTCACTGAATCATGATACATGTGAGGCTTTAGCATACAGGAGAGGCTTTCTTATTGATAGATTTGCTAAATCCATTCCACACCAAGGACTGGCACCGTCATGTAATAACTAG
Protein sequence	>Msa0722680 MSAFSKLSGTTVLEHCNFTLAVAVILVAQFNMKLVLGISMRNTTLGLTCSKEGRVLVSCHCSLNHDTCEALAYRRGFLIDRFAKSIPHQGLAPSCNN*