Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0110270 | KEH15532.1 | 72.581 | 62 | 11 | 1 | 1 | 56 | 247 | 308 | 2.74e-17 | 84.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0110270 | A0A072TDQ4 | 72.581 | 62 | 11 | 1 | 1 | 56 | 247 | 308 | 1.31e-17 | 84.0 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015750 | Msa0110270 | 0.874590 | 5.997569e-68 | -8.615850e-47 |
| Msa0016570 | Msa0110270 | 0.941127 | 7.135110e-101 | -8.615850e-47 |
| Msa0016580 | Msa0110270 | 0.964118 | 6.320340e-123 | -8.615850e-47 |
| Msa0064970 | Msa0110270 | 0.878852 | 2.007467e-69 | -8.615850e-47 |
| Msa0102070 | Msa0110270 | 0.832750 | 7.908953e-56 | -8.615850e-47 |
| Msa0110260 | Msa0110270 | 0.913419 | 6.289481e-84 | -8.615850e-47 |
| Msa0110270 | Msa0120300 | 0.804837 | 1.783268e-49 | -8.615850e-47 |
| Msa0110270 | Msa0124100 | 0.810698 | 1.012955e-50 | -8.615850e-47 |
| Msa0110270 | Msa0124150 | 0.803020 | 4.253680e-49 | -8.615850e-47 |
| Msa0110270 | Msa0142220 | 0.857629 | 1.432640e-62 | -8.615850e-47 |
| Msa0110270 | Msa0153910 | 0.831654 | 1.476739e-55 | -8.615850e-47 |
| Msa0110270 | Msa0197010 | 0.800785 | 1.224738e-48 | -8.615850e-47 |
| Msa0110270 | Msa0200000 | 0.863584 | 2.243701e-64 | -8.615850e-47 |
| Msa0110270 | Msa0227480 | 0.828463 | 8.864382e-55 | -8.615850e-47 |
| Msa0110270 | Msa0232850 | 0.832885 | 7.324163e-56 | -8.615850e-47 |
| Msa0110270 | Msa0252970 | 0.823819 | 1.128205e-53 | -8.615850e-47 |
| Msa0110270 | Msa0266720 | 0.807124 | 5.890715e-50 | -8.615850e-47 |
| Msa0110270 | Msa0267000 | 0.825253 | 5.185052e-54 | -8.615850e-47 |
| Msa0110270 | Msa0267010 | 0.836767 | 7.716122e-57 | -8.615850e-47 |
| Msa0110270 | Msa0270520 | 0.846625 | 1.931680e-59 | -8.615850e-47 |
| Msa0110270 | Msa0273100 | 0.817962 | 2.511721e-52 | -8.615850e-47 |
| Msa0110270 | Msa0304170 | 0.852690 | 3.911583e-61 | -8.615850e-47 |
| Msa0110270 | Msa0306460 | 0.880777 | 4.148933e-70 | -8.615850e-47 |
| Msa0110270 | Msa0309020 | 0.819930 | 8.964809e-53 | -8.615850e-47 |
| Msa0110270 | Msa0342240 | 0.847355 | 1.218820e-59 | -8.615850e-47 |
| Msa0110270 | Msa0349540 | 0.816109 | 6.550065e-52 | -8.615850e-47 |
| Msa0110270 | Msa0360510 | 0.832483 | 9.211324e-56 | -8.615850e-47 |
| Msa0110270 | Msa0362420 | 0.828575 | 8.330680e-55 | -8.615850e-47 |
| Msa0110270 | Msa0363300 | 0.803752 | 3.000359e-49 | -8.615850e-47 |
| Msa0110270 | Msa0365500 | 0.810215 | 1.287957e-50 | -8.615850e-47 |
| Msa0110270 | Msa0369730 | 0.802012 | 6.865970e-49 | -8.615850e-47 |
| Msa0110270 | Msa0384800 | 0.816265 | 6.044896e-52 | -8.615850e-47 |
| Msa0110270 | Msa0394970 | 0.815922 | 7.213017e-52 | -8.615850e-47 |
| Msa0110270 | Msa0414410 | 0.836986 | 6.785170e-57 | -8.615850e-47 |
| Msa0110270 | Msa0414420 | 0.852609 | 4.126950e-61 | -8.615850e-47 |
| Msa0110270 | Msa0435230 | 0.807113 | 5.922751e-50 | -8.615850e-47 |
| Msa0110270 | Msa0438620 | 0.822146 | 2.768179e-53 | -8.615850e-47 |
| Msa0110270 | Msa0444130 | 0.861650 | 8.841225e-64 | -8.615850e-47 |
| Msa0110270 | Msa0450000 | 0.836472 | 9.174240e-57 | -8.615850e-47 |
| Msa0110270 | Msa0452900 | 0.856613 | 2.858905e-62 | -8.615850e-47 |
| Msa0110270 | Msa0453120 | 0.811879 | 5.614048e-51 | -8.615850e-47 |
| Msa0110270 | Msa0459160 | 0.807131 | 5.872166e-50 | -8.615850e-47 |
| Msa0110270 | Msa0485370 | 0.801481 | 8.823711e-49 | -8.615850e-47 |
| Msa0110270 | Msa0491590 | 0.832653 | 8.359334e-56 | -8.615850e-47 |
| Msa0110270 | Msa0502340 | 0.818157 | 2.269596e-52 | -8.615850e-47 |
| Msa0110270 | Msa0502760 | 0.846194 | 2.530830e-59 | -8.615850e-47 |
| Msa0110270 | Msa0506270 | 0.802934 | 4.432404e-49 | -8.615850e-47 |
| Msa0110270 | Msa0544240 | 0.804789 | 1.824907e-49 | -8.615850e-47 |
| Msa0110270 | Msa0564890 | 0.827325 | 1.665013e-54 | -8.615850e-47 |
| Msa0110270 | Msa0589780 | 0.816464 | 5.456713e-52 | -8.615850e-47 |
| Msa0110270 | Msa0592680 | 0.800243 | 1.579121e-48 | -8.615850e-47 |
| Msa0110270 | Msa0596150 | 0.820605 | 6.279459e-53 | -8.615850e-47 |
| Msa0110270 | Msa0604350 | 0.830804 | 2.389243e-55 | -8.615850e-47 |
| Msa0110270 | Msa0636170 | 0.841866 | 3.664155e-58 | -8.615850e-47 |
| Msa0110270 | Msa0639610 | 0.818408 | 1.990923e-52 | -8.615850e-47 |
| Msa0110270 | Msa0668260 | 0.822594 | 2.178769e-53 | -8.615850e-47 |
| Msa0110270 | Msa0678930 | 0.823497 | 1.341751e-53 | -8.615850e-47 |
| Msa0110270 | Msa0694370 | 0.829245 | 5.734647e-55 | -8.615850e-47 |
| Msa0110270 | Msa0697120 | 0.810387 | 1.181974e-50 | -8.615850e-47 |
| Msa0110270 | Msa0722680 | 0.801844 | 7.434512e-49 | -8.615850e-47 |
| Msa0110270 | Msa0726140 | 0.823509 | 1.332825e-53 | -8.615850e-47 |
| Msa0110270 | Msa0729800 | 0.865181 | 7.111313e-65 | -8.615850e-47 |
| Msa0110270 | Msa0731550 | 0.805750 | 1.147922e-49 | -8.615850e-47 |
| Msa0110270 | Msa0733290 | 0.867227 | 1.598796e-65 | -8.615850e-47 |
| Msa0110270 | Msa0741990 | 0.853385 | 2.474276e-61 | -8.615850e-47 |
| Msa0110270 | Msa0743810 | 0.867862 | 1.000876e-65 | -8.615850e-47 |
| Msa0110270 | Msa0745060 | 0.839405 | 1.616127e-57 | -8.615850e-47 |
| Msa0110270 | Msa0764960 | 0.814546 | 1.458213e-51 | -8.615850e-47 |
| Msa0110270 | Msa0766630 | 0.828155 | 1.052165e-54 | -8.615850e-47 |
| Msa0110270 | Msa0767720 | 0.847672 | 9.969616e-60 | -8.615850e-47 |
| Msa0110270 | Msa0768370 | 0.803082 | 4.130771e-49 | -8.615850e-47 |
| Msa0110270 | Msa0775300 | 0.808852 | 2.527039e-50 | -8.615850e-47 |
| Msa0110270 | Msa0777790 | 0.801884 | 7.295154e-49 | -8.615850e-47 |
| Msa0110270 | Msa0783160 | 0.846820 | 1.707616e-59 | -8.615850e-47 |
| Msa0110270 | Msa0787600 | 0.814694 | 1.352268e-51 | -8.615850e-47 |
| Msa0110270 | Msa0789250 | 0.844101 | 9.314052e-59 | -8.615850e-47 |
| Msa0110270 | Msa0808010 | 0.845992 | 2.872096e-59 | -8.615850e-47 |
| Msa0110270 | Msa0808020 | 0.841077 | 5.913928e-58 | -8.615850e-47 |
| Msa0110270 | Msa0810540 | 0.803715 | 3.054656e-49 | -8.615850e-47 |
| Msa0110270 | Msa0811080 | 0.861622 | 9.014526e-64 | -8.615850e-47 |
| Msa0110270 | Msa0811270 | 0.815241 | 1.022386e-51 | -8.615850e-47 |
| Msa0110270 | Msa0813400 | 0.816880 | 4.401152e-52 | -8.615850e-47 |
| Msa0110270 | Msa0820850 | 0.812884 | 3.386239e-51 | -8.615850e-47 |
| Msa0110270 | Msa0825860 | 0.830105 | 3.541311e-55 | -8.615850e-47 |
| Msa0110270 | Msa0846210 | 0.827961 | 1.171415e-54 | -8.615850e-47 |
| Msa0110270 | Msa0846330 | 0.816119 | 6.515776e-52 | -8.615850e-47 |
| Msa0110270 | Msa0846350 | 0.842629 | 2.300947e-58 | -8.615850e-47 |
| Msa0110270 | Msa0848060 | 0.812656 | 3.798294e-51 | -8.615850e-47 |
| Msa0110270 | Msa0848960 | 0.822335 | 2.503036e-53 | -8.615850e-47 |
| Msa0110270 | Msa0851390 | 0.803067 | 4.159598e-49 | -8.615850e-47 |
| Msa0110270 | Msa0858980 | 0.836201 | 1.075040e-56 | -8.615850e-47 |
| Msa0110270 | Msa0861130 | 0.811232 | 7.759308e-51 | -8.615850e-47 |
| Msa0110270 | Msa0865320 | 0.809502 | 1.833030e-50 | -8.615850e-47 |
| Msa0110270 | Msa0872230 | 0.805881 | 1.077362e-49 | -8.615850e-47 |
| Msa0110270 | Msa0885290 | 0.810945 | 8.953113e-51 | -8.615850e-47 |
| Msa0110270 | Msa0886750 | 0.813818 | 2.110777e-51 | -8.615850e-47 |
| Msa0110270 | Msa0888370 | 0.845151 | 4.855674e-59 | -8.615850e-47 |
| Msa0110270 | Msa0889850 | 0.812506 | 4.097024e-51 | -8.615850e-47 |
| Msa0110270 | Msa0896080 | 0.810934 | 9.004219e-51 | -8.615850e-47 |
| Msa0110270 | Msa0899450 | 0.807673 | 4.506396e-50 | -8.615850e-47 |
| Msa0110270 | Msa0907260 | 0.823372 | 1.434998e-53 | -8.615850e-47 |
| Msa0110270 | Msa0952350 | 0.809309 | 2.016365e-50 | -8.615850e-47 |
| Msa0110270 | Msa0954910 | 0.840546 | 8.149005e-58 | -8.615850e-47 |
| Msa0110270 | Msa0956270 | 0.829345 | 5.422905e-55 | -8.615850e-47 |
| Msa0110270 | Msa0967280 | 0.810089 | 1.371058e-50 | -8.615850e-47 |
| Msa0110270 | Msa0976670 | 0.824010 | 1.017339e-53 | -8.615850e-47 |
| Msa0110270 | Msa0979850 | 0.820264 | 7.519655e-53 | -8.615850e-47 |
| Msa0110270 | Msa0981370 | 0.820503 | 6.628067e-53 | -8.615850e-47 |
| Msa0110270 | Msa0988370 | 0.809210 | 2.117262e-50 | -8.615850e-47 |
| Msa0110270 | Msa0989060 | 0.831531 | 1.583826e-55 | -8.615850e-47 |
| Msa0110270 | Msa1014290 | 0.803015 | 4.264477e-49 | -8.615850e-47 |
| Msa0110270 | Msa1016280 | 0.834626 | 2.688744e-56 | -8.615850e-47 |
| Msa0110270 | Msa1023050 | 0.804816 | 1.801581e-49 | -8.615850e-47 |
| Msa0110270 | Msa1025360 | 0.844067 | 9.508215e-59 | -8.615850e-47 |
| Msa0110270 | Msa1025370 | 0.822815 | 1.935381e-53 | -8.615850e-47 |
| Msa0110270 | Msa1029240 | 0.808023 | 3.796882e-50 | -8.615850e-47 |
| Msa0110270 | Msa1044830 | 0.831264 | 1.841749e-55 | -8.615850e-47 |
| Msa0110270 | Msa1044840 | 0.843746 | 1.159161e-58 | -8.615850e-47 |
| Msa0110270 | Msa1065570 | 0.823487 | 1.349350e-53 | -8.615850e-47 |
| Msa0110270 | Msa1092990 | 0.850631 | 1.498999e-60 | -8.615850e-47 |
| Msa0110270 | Msa1100980 | 0.832758 | 7.875183e-56 | -8.615850e-47 |
| Msa0110270 | Msa1109440 | 0.819965 | 8.800619e-53 | -8.615850e-47 |
| Msa0110270 | Msa1111150 | 0.836486 | 9.099634e-57 | -8.615850e-47 |
| Msa0110270 | Msa1114030 | 0.800893 | 1.163862e-48 | -8.615850e-47 |
| Msa0110270 | Msa1121200 | 0.819378 | 1.198838e-52 | -8.615850e-47 |
| Msa0110270 | Msa1121480 | 0.805536 | 1.272894e-49 | -8.615850e-47 |
| Msa0110270 | Msa1121490 | 0.807637 | 4.585544e-50 | -8.615850e-47 |
| Msa0110270 | Msa1135220 | 0.805385 | 1.369526e-49 | -8.615850e-47 |
| Msa0110270 | Msa1135740 | 0.811305 | 7.482105e-51 | -8.615850e-47 |
| Msa0110270 | Msa1136090 | 0.806415 | 8.317757e-50 | -8.615850e-47 |
| Msa0110270 | Msa1137690 | 0.801846 | 7.425175e-49 | -8.615850e-47 |
| Msa0110270 | Msa1146110 | 0.875248 | 3.577535e-68 | -8.615850e-47 |
| Msa0110270 | Msa1148020 | 0.809495 | 1.839377e-50 | -8.615850e-47 |
| Msa0110270 | Msa1150370 | 0.801382 | 9.244872e-49 | -8.615850e-47 |
| Msa0110270 | Msa1187940 | 0.834010 | 3.837622e-56 | -8.615850e-47 |
| Msa0110270 | Msa1187950 | 0.854397 | 1.264795e-61 | -8.615850e-47 |
| Msa0110270 | Msa1192640 | 0.831968 | 1.235804e-55 | -8.615850e-47 |
| Msa0110270 | Msa1246880 | 0.801054 | 1.079170e-48 | -8.615850e-47 |
| Msa0110270 | Msa1248300 | 0.822811 | 1.939476e-53 | -8.615850e-47 |
| Msa0110270 | Msa1260100 | 0.803641 | 3.164247e-49 | -8.615850e-47 |
| Msa0110270 | Msa1263300 | 0.806019 | 1.008025e-49 | -8.615850e-47 |
| Msa0110270 | Msa1269670 | 0.835691 | 1.447754e-56 | -8.615850e-47 |
| Msa0110270 | Msa1270230 | 0.860214 | 2.413805e-63 | -8.615850e-47 |
| Msa0110270 | Msa1270240 | 0.835361 | 1.755049e-56 | -8.615850e-47 |
| Msa0110270 | Msa1270270 | 0.818561 | 1.837987e-52 | -8.615850e-47 |
| Msa0110270 | Msa1273230 | 0.817306 | 3.531674e-52 | -8.615850e-47 |
| Msa0110270 | Msa1283330 | 0.859565 | 3.788135e-63 | -8.615850e-47 |
| Msa0110270 | Msa1284680 | 0.823889 | 1.085923e-53 | -8.615850e-47 |
| Msa0110270 | Msa1285930 | 0.848704 | 5.172601e-60 | -8.615850e-47 |
| Msa0110270 | Msa1287800 | 0.848088 | 7.657456e-60 | -8.615850e-47 |
| Msa0110270 | Msa1287810 | 0.855054 | 8.160569e-62 | -8.615850e-47 |
| Msa0110270 | Msa1289420 | 0.810889 | 9.207938e-51 | -8.615850e-47 |
| Msa0110270 | Msa1289430 | 0.824869 | 6.389715e-54 | -8.615850e-47 |
| Msa0110270 | Msa1320300 | 0.846875 | 1.650402e-59 | -8.615850e-47 |
| Msa0110270 | Msa1340590 | 0.855874 | 4.705145e-62 | -8.615850e-47 |
| Msa0110270 | Msa1344520 | 0.859959 | 2.882119e-63 | -8.615850e-47 |
| Msa0110270 | Msa1360610 | 0.800481 | 1.412096e-48 | -8.615850e-47 |
| Msa0110270 | Msa1366810 | 0.801271 | 9.742262e-49 | -8.615850e-47 |
| Msa0110270 | Msa1382690 | 0.808323 | 3.277827e-50 | -8.615850e-47 |
| Msa0110270 | Msa1397580 | 0.849992 | 2.264387e-60 | -8.615850e-47 |
| Msa0110270 | Msa1402790 | 0.807914 | 4.004160e-50 | -8.615850e-47 |
| Msa0110270 | Msa1404000 | 0.822220 | 2.661231e-53 | -8.615850e-47 |
| Msa0110270 | Msa1415310 | 0.800011 | 1.760522e-48 | -8.615850e-47 |
| Msa0110270 | Msa1422180 | 0.857566 | 1.495925e-62 | -8.615850e-47 |
| Msa0110270 | Msa1433120 | 0.837676 | 4.516268e-57 | -8.615850e-47 |
| Msa0110270 | Msa1451570 | 0.804388 | 2.212823e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCTAGTTCATAATCTTT+TGG | 0.250279 | 1_3:-22906888 | None:intergenic |
| AGTTGAGATAAAGAGGAAAA+TGG | 0.370037 | 1_3:+22906764 | Msa0110270:CDS |
| ACCAAAAGATTATGAACTAG+AGG | 0.481301 | 1_3:+22906887 | Msa0110270:CDS |
| ATACATCAAATATGAATCCA+AGG | 0.538148 | 1_3:+22906862 | Msa0110270:CDS |
| GATGCAGAGCTGTAGAAGGC+TGG | 0.573955 | 1_3:+22906789 | Msa0110270:intron |
| CCAAAAGATTATGAACTAGA+GGG | 0.607622 | 1_3:+22906888 | Msa0110270:CDS |
| TACATCAAATATGAATCCAA+GGG | 0.613150 | 1_3:+22906863 | Msa0110270:CDS |
| TGATTTAGAAATACTCTCGA+AGG | 0.636930 | 1_3:+22906839 | Msa0110270:CDS |
| AGGCTGGTAATGATAGCGAA+AGG | 0.640851 | 1_3:+22906805 | Msa0110270:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATACATCAAATATGAATCCA+AGG | + | chr1_3:22906862-22906881 | Msa0110270:CDS | 25.0% |
| ! | TACATCAAATATGAATCCAA+GGG | + | chr1_3:22906863-22906882 | Msa0110270:CDS | 25.0% |
| GTGAAAAAGTTGAGATAAAG+AGG | + | chr1_3:22906757-22906776 | Msa0110270:CDS | 30.0% | |
| AGTTGAGATAAAGAGGAAAA+TGG | + | chr1_3:22906764-22906783 | Msa0110270:CDS | 30.0% | |
| TGATTTAGAAATACTCTCGA+AGG | + | chr1_3:22906839-22906858 | Msa0110270:CDS | 30.0% | |
| ACCAAAAGATTATGAACTAG+AGG | + | chr1_3:22906887-22906906 | Msa0110270:CDS | 30.0% | |
| CCAAAAGATTATGAACTAGA+GGG | + | chr1_3:22906888-22906907 | Msa0110270:CDS | 30.0% | |
| !!! | TCATAATCTTTTGGTTCCCT+TGG | - | chr1_3:22906882-22906901 | None:intergenic | 35.0% |
| !! | CCCTCTAGTTCATAATCTTT+TGG | - | chr1_3:22906891-22906910 | None:intergenic | 35.0% |
| ! | AGGCTGGTAATGATAGCGAA+AGG | + | chr1_3:22906805-22906824 | Msa0110270:CDS | 45.0% |
| ! | GGTTGATGCAGAGCTGTAGA+AGG | + | chr1_3:22906785-22906804 | Msa0110270:intron | 50.0% |
| GATGCAGAGCTGTAGAAGGC+TGG | + | chr1_3:22906789-22906808 | Msa0110270:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 22906720 | 22906926 | 22906720 | ID=Msa0110270;Name=Msa0110270 |
| chr1_3 | mRNA | 22906720 | 22906926 | 22906720 | ID=Msa0110270-mRNA-1;Parent=Msa0110270;Name=Msa0110270-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|62 |
| chr1_3 | exon | 22906720 | 22906785 | 22906720 | ID=Msa0110270-mRNA-1:exon:8093;Parent=Msa0110270-mRNA-1 |
| chr1_3 | exon | 22906804 | 22906926 | 22906804 | ID=Msa0110270-mRNA-1:exon:8094;Parent=Msa0110270-mRNA-1 |
| chr1_3 | CDS | 22906720 | 22906785 | 22906720 | ID=Msa0110270-mRNA-1:cds;Parent=Msa0110270-mRNA-1 |
| chr1_3 | CDS | 22906804 | 22906926 | 22906804 | ID=Msa0110270-mRNA-1:cds;Parent=Msa0110270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
