AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0725820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725820 MtrunA17_Chr5g0417571 89.000 200 19 2 1 198 1 199 3.72e-115 330
Msa0725820 MtrunA17_Chr5g0417601 69.608 204 57 2 1 199 1 204 3.45e-90 269
Msa0725820 MtrunA17_Chr5g0417551 71.066 197 39 3 1 193 1 183 3.25e-84 254
Msa0725820 MtrunA17_Chr5g0417561 65.025 203 44 6 1 199 1 180 1.48e-72 224
Msa0725820 MtrunA17_Chr4g0042481 47.196 214 71 8 2 203 4 187 1.02e-48 165
Msa0725820 MtrunA17_Chr7g0217581 45.588 204 81 7 4 194 103 289 1.02e-40 145
Msa0725820 MtrunA17_Chr6g0453321 42.784 194 98 5 6 194 8 193 9.93e-40 141
Msa0725820 MtrunA17_Chr3g0092111 42.000 200 106 6 7 203 9 201 3.09e-37 134
Msa0725820 MtrunA17_Chr6g0460731 52.252 111 46 3 97 203 53 160 1.89e-34 125
Msa0725820 MtrunA17_Chr7g0254161 76.923 65 15 0 126 190 90 154 1.20e-33 124
Msa0725820 MtrunA17_Chr8g0355261 73.846 65 17 0 126 190 107 171 1.24e-33 124
Msa0725820 MtrunA17_Chr6g0460721 50.000 110 48 3 98 203 47 153 1.21e-31 117
Msa0725820 MtrunA17_Chr2g0283811 70.000 60 18 0 131 190 41 100 6.42e-31 110
Msa0725820 MtrunA17_Chr2g0326001 63.636 66 23 1 126 190 28 93 1.03e-26 103
Msa0725820 MtrunA17_Chr3g0127201 57.143 70 30 0 124 193 14 83 3.48e-26 102
Msa0725820 MtrunA17_Chr8g0374111 63.934 61 22 0 129 189 21 81 9.24e-26 99.4
Msa0725820 MtrunA17_Chr8g0341671 62.500 64 23 1 126 188 8 71 1.80e-25 98.6
Msa0725820 MtrunA17_Chr2g0328021 55.714 70 31 0 121 190 46 115 3.15e-25 99.4
Msa0725820 MtrunA17_Chr4g0030051 61.538 65 24 1 126 189 29 93 9.08e-25 98.2
Msa0725820 MtrunA17_Chr4g0010151 48.684 76 39 0 115 190 60 135 9.38e-25 99.4
Msa0725820 MtrunA17_Chr8g0346551 60.000 65 25 1 132 195 19 83 9.79e-25 97.8
Msa0725820 MtrunA17_Chr3g0126201 57.746 71 28 2 122 190 34 104 2.74e-24 97.8
Msa0725820 MtrunA17_Chr8g0374251 67.857 56 18 0 135 190 66 121 3.80e-24 97.4
Msa0725820 MtrunA17_Chr4g0061761 67.925 53 17 0 135 187 38 90 6.09e-24 96.7
Msa0725820 MtrunA17_Chr1g0188851 59.375 64 25 1 127 189 32 95 8.26e-24 95.9
Msa0725820 MtrunA17_Chr7g0223311 70.588 51 15 0 139 189 79 129 8.53e-24 97.1
Msa0725820 MtrunA17_Chr1g0177251 63.333 60 21 1 132 190 6 65 9.39e-24 95.5
Msa0725820 MtrunA17_Chr4g0047001 62.500 64 23 1 139 201 57 120 1.15e-23 96.7
Msa0725820 MtrunA17_Chr8g0367161 55.714 70 31 0 119 188 14 83 2.19e-23 95.5
Msa0725820 MtrunA17_Chr4g0047761 67.925 53 17 0 139 191 74 126 2.55e-23 94.7
Msa0725820 MtrunA17_Chr4g0031411 61.404 57 22 0 133 189 71 127 2.92e-23 95.1
Msa0725820 MtrunA17_Chr2g0308011 68.000 50 16 0 136 185 36 85 3.07e-23 92.4
Msa0725820 MtrunA17_Chr2g0282051 47.500 80 42 0 112 191 21 100 4.18e-23 93.6
Msa0725820 MtrunA17_Chr5g0411951 65.455 55 19 0 133 187 39 93 6.38e-23 94.0
Msa0725820 MtrunA17_Chr7g0237921 63.462 52 19 0 139 190 78 129 6.88e-22 91.3
Msa0725820 MtrunA17_Chr2g0282511 66.000 50 17 0 136 185 44 93 1.36e-21 90.1
Msa0725820 MtrunA17_Chr2g0282441 68.627 51 16 0 139 189 27 77 1.44e-21 90.5
Msa0725820 MtrunA17_Chr3g0117601 38.136 118 55 2 70 187 12 111 2.06e-21 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725820 AT5G39660.3 38.318 214 109 6 2 203 1 203 1.59e-36 132
Msa0725820 AT5G39660.1 38.318 214 109 6 2 203 1 203 1.59e-36 132
Msa0725820 AT5G39660.2 38.318 214 109 6 2 203 1 203 1.59e-36 132
Msa0725820 AT3G47500.1 53.390 118 40 2 86 203 72 174 7.61e-35 128
Msa0725820 AT1G29160.1 65.217 69 21 1 122 190 50 115 4.45e-33 117
Msa0725820 AT5G62430.1 63.855 83 29 1 122 203 38 120 6.98e-33 120
Msa0725820 AT2G34140.1 54.878 82 34 1 109 190 33 111 6.75e-32 114
Msa0725820 AT1G69570.1 71.667 60 17 0 131 190 126 185 1.22e-31 119
Msa0725820 AT1G26790.1 64.789 71 25 0 131 201 105 175 2.18e-31 117
Msa0725820 AT5G60200.1 61.538 65 25 0 130 194 48 112 2.44e-26 102
Msa0725820 AT1G51700.1 60.000 65 25 1 125 188 20 84 5.35e-26 99.8
Msa0725820 AT3G45610.1 61.538 65 25 0 130 194 33 97 2.53e-25 99.0
Msa0725820 AT2G37590.1 50.000 74 37 0 117 190 69 142 2.73e-25 100
Msa0725820 AT4G24060.1 59.091 66 27 0 125 190 41 106 3.23e-25 100
Msa0725820 AT3G55370.1 39.048 105 63 1 98 202 38 141 9.45e-25 99.4
Msa0725820 AT3G55370.3 39.048 105 63 1 98 202 83 186 1.09e-24 99.8
Msa0725820 AT3G55370.2 39.048 105 63 1 98 202 38 141 1.22e-24 99.4
Msa0725820 AT3G61850.2 60.317 63 25 0 132 194 57 119 1.82e-24 97.8
Msa0725820 AT3G61850.3 60.317 63 25 0 132 194 57 119 1.82e-24 97.8
Msa0725820 AT3G61850.1 60.317 63 25 0 132 194 69 131 2.27e-24 97.8
Msa0725820 AT3G61850.5 60.317 63 25 0 132 194 57 119 2.66e-24 97.8
Msa0725820 AT3G61850.4 60.317 63 25 0 132 194 69 131 3.52e-24 97.8
Msa0725820 AT2G28810.2 43.182 88 48 1 101 188 43 128 3.54e-24 97.8
Msa0725820 AT5G60850.1 65.517 58 20 0 135 192 49 106 3.93e-24 97.4
Msa0725820 AT3G50410.1 69.231 52 16 0 137 188 30 81 3.95e-24 96.3
Msa0725820 AT2G28810.1 43.182 88 48 1 101 188 60 145 5.48e-24 97.4
Msa0725820 AT3G21270.1 70.588 51 15 0 139 189 31 81 6.47e-24 94.4
Msa0725820 AT1G07640.1 54.286 70 26 1 120 189 10 73 7.30e-24 95.9
Msa0725820 AT1G07640.2 54.286 70 26 1 120 189 66 129 8.39e-24 96.7
Msa0725820 AT1G07640.3 54.286 70 26 1 120 189 74 137 9.71e-24 96.7
Msa0725820 AT2G28510.1 66.071 56 19 0 139 194 50 105 2.49e-23 94.7
Msa0725820 AT5G66940.1 44.681 94 39 1 95 188 3 83 4.20e-23 92.8
Msa0725820 AT1G64620.1 56.250 64 27 1 122 185 35 97 9.70e-23 94.4
Msa0725820 AT1G47655.1 48.718 78 37 1 108 185 3 77 1.01e-22 91.3
Msa0725820 AT3G52440.1 65.385 52 18 0 139 190 27 78 1.28e-22 92.0
Msa0725820 AT3G52440.2 65.385 52 18 0 139 190 46 97 1.70e-22 92.0
Msa0725820 AT5G65590.1 48.387 93 46 1 100 192 6 96 1.78e-22 92.8
Msa0725820 AT1G28310.1 67.308 52 17 0 139 190 29 80 1.79e-22 92.8
Msa0725820 AT1G28310.2 67.308 52 17 0 139 190 53 104 2.16e-22 93.2
Msa0725820 AT4G38000.1 58.209 67 26 2 125 190 30 95 4.66e-22 90.5
Msa0725820 AT4G00940.1 57.627 59 25 0 132 190 63 121 6.49e-22 91.3
Msa0725820 AT4G00940.3 57.627 59 25 0 132 190 63 121 6.49e-22 91.3
Msa0725820 AT4G00940.2 57.627 59 25 0 132 190 63 121 6.49e-22 91.3
Msa0725820 AT1G21340.1 66.000 50 17 0 139 188 40 89 6.79e-22 90.5
Msa0725820 AT5G02460.1 58.621 58 24 0 133 190 91 148 1.01e-21 92.0
Msa0725820 AT2G46590.1 60.377 53 21 0 132 184 63 115 2.39e-21 90.5
Msa0725820 AT2G46590.2 60.377 53 21 0 132 184 75 127 3.02e-21 90.5
Msa0725820 AT5G62940.1 69.388 49 15 0 139 187 75 123 5.33e-21 89.7
Msa0725820 AT4G21050.1 54.688 64 29 0 125 188 12 75 2.30e-20 85.1
Msa0725820 AT4G21040.1 63.462 52 19 0 139 190 27 78 1.01e-18 81.3
Msa0725820 AT4G21080.1 63.462 52 19 0 139 190 27 78 4.06e-18 80.1

Find 52 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTAAAGTTAGTTTATT+TGG 0.151164 5_1:-25718627 None:intergenic
AACCAAATAAACTAACTTTA+AGG 0.169523 5_1:+25718625 Msa0725820:three_prime_UTR
CCACCATAGAATGCTATATT+TGG 0.230148 5_1:-25718552 None:intergenic
ACAACTTGGTCTGGTTTCTT+TGG 0.248772 5_1:-25718160 None:intergenic
CTCCAGAAGTCCAGTATCTT+TGG 0.293736 5_1:-25718273 None:intergenic
TGCCAAAGATACTGGACTTC+TGG 0.300675 5_1:+25718271 Msa0725820:CDS
TTCCATGAGATGATTTGATT+TGG 0.304050 5_1:+25718750 Msa0725820:three_prime_UTR
AATGCTATATTTGGATAGAT+TGG 0.337846 5_1:-25718543 None:intergenic
ATGAGTACTGTCTTGACATT+TGG 0.342898 5_1:+25718384 Msa0725820:exon
TATCCAAATATAGCATTCTA+TGG 0.352424 5_1:+25718549 Msa0725820:three_prime_UTR
AGTGAAGTTGAAGACATAAT+AGG 0.361504 5_1:-25718417 None:intergenic
GAGATGATTTGATTTGGAAT+AGG 0.387321 5_1:+25718756 Msa0725820:three_prime_UTR
ACATGATGCAAAGAGATATA+AGG 0.388068 5_1:+25717306 Msa0725820:CDS
ATTCTGAATCCTCTGTGGTT+TGG 0.395071 5_1:-25718021 None:intergenic
AGCATTCTATGGTGGTTGTT+TGG 0.399605 5_1:+25718560 Msa0725820:three_prime_UTR
GGACATGGAACAACTTGGTC+TGG 0.406395 5_1:-25718169 None:intergenic
GTGGTTGTTTGGTACCATCT+TGG 0.412555 5_1:+25718571 Msa0725820:three_prime_UTR
ACCACGAGAAATATGCTTAT+AGG 0.437951 5_1:+25718298 Msa0725820:CDS
ATCTTTCGTTCAAAGAATGA+TGG 0.447271 5_1:+25718672 Msa0725820:three_prime_UTR
CTAACGCTGCAGCTTTATCA+AGG 0.467712 5_1:+25718718 Msa0725820:three_prime_UTR
GTTGAAGAATTCACTTCAAG+TGG 0.481761 5_1:-25717280 None:intergenic
TAAGGAAACATTCAAGAGAT+GGG 0.488389 5_1:+25718643 Msa0725820:three_prime_UTR
AAATCTTCATGAGCAATAGT+AGG 0.488963 5_1:-25717217 None:intergenic
TTCCAAATCAAATCATCTCA+TGG 0.489753 5_1:-25718752 None:intergenic
TACTGTCTTGACATTTGGAT+CGG 0.502009 5_1:+25718389 Msa0725820:three_prime_UTR
AAAAGAACTCATCGAGGATG+AGG 0.512018 5_1:+25717991 Msa0725820:CDS
TTAAGGAAACATTCAAGAGA+TGG 0.512653 5_1:+25718642 Msa0725820:three_prime_UTR
GACGTCTCTGTTTCAGATGA+GGG 0.514212 5_1:-25718082 None:intergenic
TTCATTAGACTGTGTTGAGA+TGG 0.528897 5_1:-25718604 None:intergenic
AGAAATATGCTTATAGGCGC+AGG 0.536173 5_1:+25718304 Msa0725820:CDS
ATATTTGGATAGATTGGCAC+AGG 0.538462 5_1:-25718537 None:intergenic
TAACACATAGCAGGATTCCA+TGG 0.544411 5_1:-25718513 None:intergenic
GAATGATGGAGGACTTTCCA+AGG 0.552945 5_1:+25718686 Msa0725820:three_prime_UTR
AGACGTCTCTGTTTCAGATG+AGG 0.571871 5_1:-25718083 None:intergenic
AAGAAGAGATTCTGTTCTTG+CGG 0.572733 5_1:-25718330 None:intergenic
ATCTTGGACATGGAACAACT+TGG 0.574876 5_1:-25718174 None:intergenic
TCAATAAGGCTTGAAGATGT+CGG 0.575632 5_1:-25718046 None:intergenic
GCCTATAAGCATATTTCTCG+TGG 0.581874 5_1:-25718299 None:intergenic
GGCACAGGATAACACATAGC+AGG 0.586834 5_1:-25718522 None:intergenic
GGATTCCATGGAAACTGTTG+AGG 0.594593 5_1:-25718501 None:intergenic
AATAGTAGGAGTAAATTCTG+AGG 0.595274 5_1:-25717203 None:intergenic
AATGATGGAGGACTTTCCAA+GGG 0.597993 5_1:+25718687 Msa0725820:three_prime_UTR
AGCGTTAGAAGCAAAACCCT+TGG 0.603793 5_1:-25718703 None:intergenic
ATGGATTGTACGCTCCAAGA+TGG 0.605225 5_1:-25718585 None:intergenic
AAGGAAACATTCAAGAGATG+GGG 0.613145 5_1:+25718644 Msa0725820:three_prime_UTR
TTTCGTTCAAAGAATGATGG+AGG 0.623392 5_1:+25718675 Msa0725820:three_prime_UTR
CGGAGATTCTGAATCCTCTG+TGG 0.629252 5_1:-25718026 None:intergenic
CAAAGATACTGGACTTCTGG+AGG 0.634659 5_1:+25718274 Msa0725820:CDS
CCAAATATAGCATTCTATGG+TGG 0.645659 5_1:+25718552 Msa0725820:three_prime_UTR
ATGTCCAAGATGCAAAAGCA+CGG 0.662046 5_1:+25718186 Msa0725820:CDS
ATCAAGAAAAGAACTCATCG+AGG 0.704142 5_1:+25717985 Msa0725820:CDS
GGCTTCATTCCAAACCACAG+AGG 0.724092 5_1:+25718012 Msa0725820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATAAGAACAAATAAAT+CGG + chr5_1:25717747-25717766 Msa0725820:intron 10.0%
!! TTATATAAGAACAAATAAAT+CGG + chr5_1:25717747-25717766 Msa0725820:intron 10.0%
!!! TTCAAATTTTTCATCTTTTT+TGG + chr5_1:25717405-25717424 Msa0725820:intron 15.0%
!! AATACATGTAGTTATATTAT+CGG + chr5_1:25717836-25717855 Msa0725820:intron 15.0%
!!! TATAAGCCAATAAAATTTTA+TGG + chr5_1:25717915-25717934 Msa0725820:intron 15.0%
!!! TTCAAATTTTTCATCTTTTT+TGG + chr5_1:25717405-25717424 Msa0725820:intron 15.0%
!! AATACATGTAGTTATATTAT+CGG + chr5_1:25717836-25717855 Msa0725820:intron 15.0%
!!! TATAAGCCAATAAAATTTTA+TGG + chr5_1:25717915-25717934 Msa0725820:intron 15.0%
!!! TATGAGCCATAAAATTTTAT+TGG - chr5_1:25717924-25717943 None:intergenic 20.0%
!!! AAATTTTATGGCTCATATTT+TGG + chr5_1:25717927-25717946 Msa0725820:intron 20.0%
!!! AGTTTGTTTATCAATTTTAG+TGG - chr5_1:25718119-25718138 None:intergenic 20.0%
!! AACCAAATAAACTAACTTTA+AGG + chr5_1:25718625-25718644 Msa0725820:three_prime_UTR 20.0%
!!! TTCCTTAAAGTTAGTTTATT+TGG - chr5_1:25718630-25718649 None:intergenic 20.0%
!!! TATGAGCCATAAAATTTTAT+TGG - chr5_1:25717924-25717943 None:intergenic 20.0%
!!! AAATTTTATGGCTCATATTT+TGG + chr5_1:25717927-25717946 Msa0725820:intron 20.0%
!!! AGTTTGTTTATCAATTTTAG+TGG - chr5_1:25718119-25718138 None:intergenic 20.0%
!! AACCAAATAAACTAACTTTA+AGG + chr5_1:25718625-25718644 Msa0725820:three_prime_UTR 20.0%
!!! TTCCTTAAAGTTAGTTTATT+TGG - chr5_1:25718630-25718649 None:intergenic 20.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5_1:25717130-25717149 Msa0725820:CDS 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5_1:25717131-25717150 Msa0725820:CDS 25.0%
! TTCTGTTCGAAAAATATACA+CGG + chr5_1:25717508-25717527 Msa0725820:intron 25.0%
!!! TATATTTTGATCCTAACATG+AGG - chr5_1:25717620-25717639 None:intergenic 25.0%
! AAACAACAGAAAATTCATGT+TGG - chr5_1:25717725-25717744 None:intergenic 25.0%
! AACAGCTATATATCAATTTC+AGG + chr5_1:25717958-25717977 Msa0725820:intron 25.0%
! AATGCTATATTTGGATAGAT+TGG - chr5_1:25718546-25718565 None:intergenic 25.0%
! TATCCAAATATAGCATTCTA+TGG + chr5_1:25718549-25718568 Msa0725820:three_prime_UTR 25.0%
! TATGGTTGAATTGTTTATCA+TGG - chr5_1:25717122-25717141 None:intergenic 25.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5_1:25717130-25717149 Msa0725820:CDS 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5_1:25717131-25717150 Msa0725820:CDS 25.0%
! TTCTGTTCGAAAAATATACA+CGG + chr5_1:25717508-25717527 Msa0725820:intron 25.0%
!!! TATATTTTGATCCTAACATG+AGG - chr5_1:25717620-25717639 None:intergenic 25.0%
! AAACAACAGAAAATTCATGT+TGG - chr5_1:25717725-25717744 None:intergenic 25.0%
! AACAGCTATATATCAATTTC+AGG + chr5_1:25717958-25717977 Msa0725820:intron 25.0%
! AATGCTATATTTGGATAGAT+TGG - chr5_1:25718546-25718565 None:intergenic 25.0%
! TATCCAAATATAGCATTCTA+TGG + chr5_1:25718549-25718568 Msa0725820:three_prime_UTR 25.0%
AATAGTAGGAGTAAATTCTG+AGG - chr5_1:25717206-25717225 None:intergenic 30.0%
AAATCTTCATGAGCAATAGT+AGG - chr5_1:25717220-25717239 None:intergenic 30.0%
ACATGATGCAAAGAGATATA+AGG + chr5_1:25717306-25717325 Msa0725820:CDS 30.0%
TTCTTCAAATAGAATCACCA+TGG - chr5_1:25717343-25717362 None:intergenic 30.0%
AAAATATGTTGCTAGCTAGT+AGG + chr5_1:25717377-25717396 Msa0725820:intron 30.0%
! GTTGATGAAATTGTTCATCA+AGG + chr5_1:25717530-25717549 Msa0725820:intron 30.0%
TATATGAGAAACCTCATGTT+AGG + chr5_1:25717606-25717625 Msa0725820:intron 30.0%
AATAGTAACAAGAACCTTGA+TGG - chr5_1:25717657-25717676 None:intergenic 30.0%
! CTCTCTTTATATTTGTTGGT+AGG - chr5_1:25717685-25717704 None:intergenic 30.0%
GCATCTCTCTTTATATTTGT+TGG - chr5_1:25717689-25717708 None:intergenic 30.0%
!! AAGAGAGATGCTTTTTATCA+AGG + chr5_1:25717698-25717717 Msa0725820:intron 30.0%
! TACATGTATTGACATCATGT+TGG - chr5_1:25717826-25717845 None:intergenic 30.0%
AGTGAAGTTGAAGACATAAT+AGG - chr5_1:25718420-25718439 None:intergenic 30.0%
TCAACTTCACTTCACAAAAA+AGG + chr5_1:25718429-25718448 Msa0725820:three_prime_UTR 30.0%
CAACTTCACTTCACAAAAAA+GGG + chr5_1:25718430-25718449 Msa0725820:three_prime_UTR 30.0%
TTAAGGAAACATTCAAGAGA+TGG + chr5_1:25718642-25718661 Msa0725820:three_prime_UTR 30.0%
TAAGGAAACATTCAAGAGAT+GGG + chr5_1:25718643-25718662 Msa0725820:three_prime_UTR 30.0%
ATCTTTCGTTCAAAGAATGA+TGG + chr5_1:25718672-25718691 Msa0725820:three_prime_UTR 30.0%
TTCCATGAGATGATTTGATT+TGG + chr5_1:25718750-25718769 Msa0725820:three_prime_UTR 30.0%
TTCCAAATCAAATCATCTCA+TGG - chr5_1:25718755-25718774 None:intergenic 30.0%
!! GAGATGATTTGATTTGGAAT+AGG + chr5_1:25718756-25718775 Msa0725820:three_prime_UTR 30.0%
AATAGTAGGAGTAAATTCTG+AGG - chr5_1:25717206-25717225 None:intergenic 30.0%
AAATCTTCATGAGCAATAGT+AGG - chr5_1:25717220-25717239 None:intergenic 30.0%
ACATGATGCAAAGAGATATA+AGG + chr5_1:25717306-25717325 Msa0725820:CDS 30.0%
TTCTTCAAATAGAATCACCA+TGG - chr5_1:25717343-25717362 None:intergenic 30.0%
AAAATATGTTGCTAGCTAGT+AGG + chr5_1:25717377-25717396 Msa0725820:intron 30.0%
! GTTGATGAAATTGTTCATCA+AGG + chr5_1:25717530-25717549 Msa0725820:intron 30.0%
TATATGAGAAACCTCATGTT+AGG + chr5_1:25717606-25717625 Msa0725820:intron 30.0%
AATAGTAACAAGAACCTTGA+TGG - chr5_1:25717657-25717676 None:intergenic 30.0%
! CTCTCTTTATATTTGTTGGT+AGG - chr5_1:25717685-25717704 None:intergenic 30.0%
GCATCTCTCTTTATATTTGT+TGG - chr5_1:25717689-25717708 None:intergenic 30.0%
!! AAGAGAGATGCTTTTTATCA+AGG + chr5_1:25717698-25717717 Msa0725820:intron 30.0%
! TACATGTATTGACATCATGT+TGG - chr5_1:25717826-25717845 None:intergenic 30.0%
AGTGAAGTTGAAGACATAAT+AGG - chr5_1:25718420-25718439 None:intergenic 30.0%
TCAACTTCACTTCACAAAAA+AGG + chr5_1:25718429-25718448 Msa0725820:three_prime_UTR 30.0%
CAACTTCACTTCACAAAAAA+GGG + chr5_1:25718430-25718449 Msa0725820:three_prime_UTR 30.0%
TTAAGGAAACATTCAAGAGA+TGG + chr5_1:25718642-25718661 Msa0725820:three_prime_UTR 30.0%
TAAGGAAACATTCAAGAGAT+GGG + chr5_1:25718643-25718662 Msa0725820:three_prime_UTR 30.0%
ATCTTTCGTTCAAAGAATGA+TGG + chr5_1:25718672-25718691 Msa0725820:three_prime_UTR 30.0%
TTCCATGAGATGATTTGATT+TGG + chr5_1:25718750-25718769 Msa0725820:three_prime_UTR 30.0%
TTCCAAATCAAATCATCTCA+TGG - chr5_1:25718755-25718774 None:intergenic 30.0%
!! GAGATGATTTGATTTGGAAT+AGG + chr5_1:25718756-25718775 Msa0725820:three_prime_UTR 30.0%
GTTGAAGAATTCACTTCAAG+TGG - chr5_1:25717283-25717302 None:intergenic 35.0%
ATCAAGAAAAGAACTCATCG+AGG + chr5_1:25717985-25718004 Msa0725820:CDS 35.0%
TCAATAAGGCTTGAAGATGT+CGG - chr5_1:25718049-25718068 None:intergenic 35.0%
!!! GAGTTTTCAGGTTCTCAATA+AGG - chr5_1:25718063-25718082 None:intergenic 35.0%
GCAAAAATTGCCAAAGATAC+TGG + chr5_1:25718263-25718282 Msa0725820:CDS 35.0%
ACCACGAGAAATATGCTTAT+AGG + chr5_1:25718298-25718317 Msa0725820:CDS 35.0%
AAGAAGAGATTCTGTTCTTG+CGG - chr5_1:25718333-25718352 None:intergenic 35.0%
! ATGAGTACTGTCTTGACATT+TGG + chr5_1:25718384-25718403 Msa0725820:exon 35.0%
TACTGTCTTGACATTTGGAT+CGG + chr5_1:25718389-25718408 Msa0725820:three_prime_UTR 35.0%
CTTCACAAAAAAGGGAATGT+TGG + chr5_1:25718438-25718457 Msa0725820:three_prime_UTR 35.0%
ATATTTGGATAGATTGGCAC+AGG - chr5_1:25718540-25718559 None:intergenic 35.0%
CCACCATAGAATGCTATATT+TGG - chr5_1:25718555-25718574 None:intergenic 35.0%
CCAAATATAGCATTCTATGG+TGG + chr5_1:25718552-25718571 Msa0725820:three_prime_UTR 35.0%
TTCATTAGACTGTGTTGAGA+TGG - chr5_1:25718607-25718626 None:intergenic 35.0%
AAGGAAACATTCAAGAGATG+GGG + chr5_1:25718644-25718663 Msa0725820:three_prime_UTR 35.0%
TTTCGTTCAAAGAATGATGG+AGG + chr5_1:25718675-25718694 Msa0725820:three_prime_UTR 35.0%
GTTGAAGAATTCACTTCAAG+TGG - chr5_1:25717283-25717302 None:intergenic 35.0%
ATCAAGAAAAGAACTCATCG+AGG + chr5_1:25717985-25718004 Msa0725820:CDS 35.0%
TCAATAAGGCTTGAAGATGT+CGG - chr5_1:25718049-25718068 None:intergenic 35.0%
!!! GAGTTTTCAGGTTCTCAATA+AGG - chr5_1:25718063-25718082 None:intergenic 35.0%
GCAAAAATTGCCAAAGATAC+TGG + chr5_1:25718263-25718282 Msa0725820:CDS 35.0%
ACCACGAGAAATATGCTTAT+AGG + chr5_1:25718298-25718317 Msa0725820:CDS 35.0%
AAGAAGAGATTCTGTTCTTG+CGG - chr5_1:25718333-25718352 None:intergenic 35.0%
! ATGAGTACTGTCTTGACATT+TGG + chr5_1:25718384-25718403 Msa0725820:exon 35.0%
TACTGTCTTGACATTTGGAT+CGG + chr5_1:25718389-25718408 Msa0725820:three_prime_UTR 35.0%
CTTCACAAAAAAGGGAATGT+TGG + chr5_1:25718438-25718457 Msa0725820:three_prime_UTR 35.0%
ATATTTGGATAGATTGGCAC+AGG - chr5_1:25718540-25718559 None:intergenic 35.0%
CCACCATAGAATGCTATATT+TGG - chr5_1:25718555-25718574 None:intergenic 35.0%
CCAAATATAGCATTCTATGG+TGG + chr5_1:25718552-25718571 Msa0725820:three_prime_UTR 35.0%
TTCATTAGACTGTGTTGAGA+TGG - chr5_1:25718607-25718626 None:intergenic 35.0%
AAGGAAACATTCAAGAGATG+GGG + chr5_1:25718644-25718663 Msa0725820:three_prime_UTR 35.0%
TTTCGTTCAAAGAATGATGG+AGG + chr5_1:25718675-25718694 Msa0725820:three_prime_UTR 35.0%
CGTTCTCCCAAAAAGCTTTA+TGG - chr5_1:25717140-25717159 None:intergenic 40.0%
CAAATAGAATCACCATGGCA+TGG - chr5_1:25717338-25717357 None:intergenic 40.0%
TGAGTTTCACTGCGATTTCT+CGG + chr5_1:25717469-25717488 Msa0725820:intron 40.0%
TGAAAGAGATCTGTCCATCA+AGG + chr5_1:25717640-25717659 Msa0725820:intron 40.0%
CTCGATGTTTGTGTATGTGT+CGG + chr5_1:25717866-25717885 Msa0725820:intron 40.0%
AAAAGAACTCATCGAGGATG+AGG + chr5_1:25717991-25718010 Msa0725820:CDS 40.0%
ATTCTGAATCCTCTGTGGTT+TGG - chr5_1:25718024-25718043 None:intergenic 40.0%
! TTTCAGATGAGGGAGTTTTC+AGG - chr5_1:25718075-25718094 None:intergenic 40.0%
! ACAACTTGGTCTGGTTTCTT+TGG - chr5_1:25718163-25718182 None:intergenic 40.0%
ATCTTGGACATGGAACAACT+TGG - chr5_1:25718177-25718196 None:intergenic 40.0%
ATGTCCAAGATGCAAAAGCA+CGG + chr5_1:25718186-25718205 Msa0725820:CDS 40.0%
!!! CAATTTTTGCAGAAGTGTCG+AGG - chr5_1:25718253-25718272 None:intergenic 40.0%
GCCTATAAGCATATTTCTCG+TGG - chr5_1:25718302-25718321 None:intergenic 40.0%
AGAAATATGCTTATAGGCGC+AGG + chr5_1:25718304-25718323 Msa0725820:CDS 40.0%
! GTTTTCCTCAACAGTTTCCA+TGG + chr5_1:25718496-25718515 Msa0725820:three_prime_UTR 40.0%
TAACACATAGCAGGATTCCA+TGG - chr5_1:25718516-25718535 None:intergenic 40.0%
AGCATTCTATGGTGGTTGTT+TGG + chr5_1:25718560-25718579 Msa0725820:three_prime_UTR 40.0%
! AATGATGGAGGACTTTCCAA+GGG + chr5_1:25718687-25718706 Msa0725820:three_prime_UTR 40.0%
CGTTCTCCCAAAAAGCTTTA+TGG - chr5_1:25717140-25717159 None:intergenic 40.0%
CAAATAGAATCACCATGGCA+TGG - chr5_1:25717338-25717357 None:intergenic 40.0%
TGAGTTTCACTGCGATTTCT+CGG + chr5_1:25717469-25717488 Msa0725820:intron 40.0%
TGAAAGAGATCTGTCCATCA+AGG + chr5_1:25717640-25717659 Msa0725820:intron 40.0%
CTCGATGTTTGTGTATGTGT+CGG + chr5_1:25717866-25717885 Msa0725820:intron 40.0%
AAAAGAACTCATCGAGGATG+AGG + chr5_1:25717991-25718010 Msa0725820:CDS 40.0%
ATTCTGAATCCTCTGTGGTT+TGG - chr5_1:25718024-25718043 None:intergenic 40.0%
! TTTCAGATGAGGGAGTTTTC+AGG - chr5_1:25718075-25718094 None:intergenic 40.0%
! ACAACTTGGTCTGGTTTCTT+TGG - chr5_1:25718163-25718182 None:intergenic 40.0%
ATCTTGGACATGGAACAACT+TGG - chr5_1:25718177-25718196 None:intergenic 40.0%
ATGTCCAAGATGCAAAAGCA+CGG + chr5_1:25718186-25718205 Msa0725820:CDS 40.0%
!!! CAATTTTTGCAGAAGTGTCG+AGG - chr5_1:25718253-25718272 None:intergenic 40.0%
GCCTATAAGCATATTTCTCG+TGG - chr5_1:25718302-25718321 None:intergenic 40.0%
AGAAATATGCTTATAGGCGC+AGG + chr5_1:25718304-25718323 Msa0725820:CDS 40.0%
! GTTTTCCTCAACAGTTTCCA+TGG + chr5_1:25718496-25718515 Msa0725820:three_prime_UTR 40.0%
TAACACATAGCAGGATTCCA+TGG - chr5_1:25718516-25718535 None:intergenic 40.0%
AGCATTCTATGGTGGTTGTT+TGG + chr5_1:25718560-25718579 Msa0725820:three_prime_UTR 40.0%
! AATGATGGAGGACTTTCCAA+GGG + chr5_1:25718687-25718706 Msa0725820:three_prime_UTR 40.0%
ATAAGGTATGTGCCATGCCA+TGG + chr5_1:25717323-25717342 Msa0725820:intron 45.0%
GAGTTTCACTGCGATTTCTC+GGG + chr5_1:25717470-25717489 Msa0725820:intron 45.0%
AGTTTCACTGCGATTTCTCG+GGG + chr5_1:25717471-25717490 Msa0725820:intron 45.0%
GACGTCTCTGTTTCAGATGA+GGG - chr5_1:25718085-25718104 None:intergenic 45.0%
AGACGTCTCTGTTTCAGATG+AGG - chr5_1:25718086-25718105 None:intergenic 45.0%
! GTGCTTTTGCATCTTGGACA+TGG - chr5_1:25718187-25718206 None:intergenic 45.0%
TGCCAAAGATACTGGACTTC+TGG + chr5_1:25718271-25718290 Msa0725820:CDS 45.0%
CTCCAGAAGTCCAGTATCTT+TGG - chr5_1:25718276-25718295 None:intergenic 45.0%
CAAAGATACTGGACTTCTGG+AGG + chr5_1:25718274-25718293 Msa0725820:CDS 45.0%
GGATTCCATGGAAACTGTTG+AGG - chr5_1:25718504-25718523 None:intergenic 45.0%
GTGGTTGTTTGGTACCATCT+TGG + chr5_1:25718571-25718590 Msa0725820:three_prime_UTR 45.0%
! ATGGATTGTACGCTCCAAGA+TGG - chr5_1:25718588-25718607 None:intergenic 45.0%
! GAATGATGGAGGACTTTCCA+AGG + chr5_1:25718686-25718705 Msa0725820:three_prime_UTR 45.0%
AGCGTTAGAAGCAAAACCCT+TGG - chr5_1:25718706-25718725 None:intergenic 45.0%
! CTAACGCTGCAGCTTTATCA+AGG + chr5_1:25718718-25718737 Msa0725820:three_prime_UTR 45.0%
ATAAGGTATGTGCCATGCCA+TGG + chr5_1:25717323-25717342 Msa0725820:intron 45.0%
GAGTTTCACTGCGATTTCTC+GGG + chr5_1:25717470-25717489 Msa0725820:intron 45.0%
AGTTTCACTGCGATTTCTCG+GGG + chr5_1:25717471-25717490 Msa0725820:intron 45.0%
GACGTCTCTGTTTCAGATGA+GGG - chr5_1:25718085-25718104 None:intergenic 45.0%
AGACGTCTCTGTTTCAGATG+AGG - chr5_1:25718086-25718105 None:intergenic 45.0%
! GTGCTTTTGCATCTTGGACA+TGG - chr5_1:25718187-25718206 None:intergenic 45.0%
TGCCAAAGATACTGGACTTC+TGG + chr5_1:25718271-25718290 Msa0725820:CDS 45.0%
CTCCAGAAGTCCAGTATCTT+TGG - chr5_1:25718276-25718295 None:intergenic 45.0%
CAAAGATACTGGACTTCTGG+AGG + chr5_1:25718274-25718293 Msa0725820:CDS 45.0%
GGATTCCATGGAAACTGTTG+AGG - chr5_1:25718504-25718523 None:intergenic 45.0%
GTGGTTGTTTGGTACCATCT+TGG + chr5_1:25718571-25718590 Msa0725820:three_prime_UTR 45.0%
! ATGGATTGTACGCTCCAAGA+TGG - chr5_1:25718588-25718607 None:intergenic 45.0%
! GAATGATGGAGGACTTTCCA+AGG + chr5_1:25718686-25718705 Msa0725820:three_prime_UTR 45.0%
AGCGTTAGAAGCAAAACCCT+TGG - chr5_1:25718706-25718725 None:intergenic 45.0%
! CTAACGCTGCAGCTTTATCA+AGG + chr5_1:25718718-25718737 Msa0725820:three_prime_UTR 45.0%
GGCTTCATTCCAAACCACAG+AGG + chr5_1:25718012-25718031 Msa0725820:CDS 50.0%
CGGAGATTCTGAATCCTCTG+TGG - chr5_1:25718029-25718048 None:intergenic 50.0%
GGACATGGAACAACTTGGTC+TGG - chr5_1:25718172-25718191 None:intergenic 50.0%
! GTGTCCGTGCTTTTGCATCT+TGG - chr5_1:25718193-25718212 None:intergenic 50.0%
GGCACAGGATAACACATAGC+AGG - chr5_1:25718525-25718544 None:intergenic 50.0%
GGCTTCATTCCAAACCACAG+AGG + chr5_1:25718012-25718031 Msa0725820:CDS 50.0%
CGGAGATTCTGAATCCTCTG+TGG - chr5_1:25718029-25718048 None:intergenic 50.0%
GGACATGGAACAACTTGGTC+TGG - chr5_1:25718172-25718191 None:intergenic 50.0%
! GTGTCCGTGCTTTTGCATCT+TGG - chr5_1:25718193-25718212 None:intergenic 50.0%
GGCACAGGATAACACATAGC+AGG - chr5_1:25718525-25718544 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 25717121 25718791 25717121 ID=Msa0725820;Name=Msa0725820
chr5_1 mRNA 25717121 25718791 25717121 ID=Msa0725820-mRNA-1;Parent=Msa0725820;Name=Msa0725820-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|1|0.5|1|1|0.5|2|404|204
chr5_1 exon 25717121 25717327 25717121 ID=Msa0725820-mRNA-1:exon:9273;Parent=Msa0725820-mRNA-1
chr5_1 exon 25717980 25718791 25717980 ID=Msa0725820-mRNA-1:exon:9274;Parent=Msa0725820-mRNA-1
chr5_1 CDS 25717121 25717327 25717121 ID=Msa0725820-mRNA-1:cds;Parent=Msa0725820-mRNA-1
chr5_1 CDS 25717980 25718387 25717980 ID=Msa0725820-mRNA-1:cds;Parent=Msa0725820-mRNA-1
chr5_1 three_prime_UTR 25718388 25718791 25718388 ID=Msa0725820-mRNA-1:three_prime_utr;Parent=Msa0725820-mRNA-1
Gene Sequence

>Msa0725820

ATGATAAACAATTCAACCATAAAGCTTTTTGGGAGAACGATTTTTCAAACACACAACACTGATGTGTCTATCAATGATTCTTCCTCAGAATTTACTCCTACTATTGCTCATGAAGATTTCTCTGATCATAGTAGTCATTCATCTTTATCTGCATCTTCTCCACTTGAAGTGAATTCTTCAACTGAACATGATGCAAAGAGATATAAGGAAACATCAAGAAAAGAACTCATCGAGGATGAGGCTTCATTCCAAACCACAGAGGATTCAGAATCTCCGACATCTTCAAGCCTTATTGAGAACCTGAAAACTCCCTCATCTGAAACAGAGACGTCTCAATCAATTTCCACTAAAATTGATAAACAAACTGATATGTCACAAGAAAAATCTCCAAAGAAACCAGACCAAGTTGTTCCATGTCCAAGATGCAAAAGCACGGACACAAAATTTTGCTACTACAACAACTACAACGTCAAACAGCCTCGACACTTCTGCAAAAATTGCCAAAGATACTGGACTTCTGGAGGAACCACGAGAAATATGCTTATAGGCGCAGGTCGCCGCAAGAACAGAATCTCTTCTTCTTTTCATTCTGATACTCACATCATCATCAAATGA
Protein sequence

>Msa0725820

MINNSTIKLFGRTIFQTHNTDVSINDSSSEFTPTIAHEDFSDHSSHSSLSASSPLEVNSSTEHDAKRYKETSRKELIEDEASFQTTEDSESPTSSSLIENLKTPSSETETSQSISTKIDKQTDMSQEKSPKKPDQVVPCPRCKSTDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGTTRNMLIGAGRRKNRISSSFHSDTHIIIK*