AlfalfaGEDB | Msa0726030

M. sativa cultivar zhongmu-4 / Msa0726030

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009825 GO:0009835 GO:0009889 GO:0009908 GO:0009987 GO:0010150 GO:0010154 GO:0010468 GO:0010556 GO:0016049 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071695 GO:0080090 GO:0090567 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0726030	AIU47058.1	98.540	274	3	1	1	273	1	274	0.0	564

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0726030	sp\|K4BNG7\|NAP2_SOLLC	70.925	227	55	4	2	218	9	234	6.12e-115	334

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0726030	A0A097PHQ6	98.540	274	3	1	1	273	1	274	0.0	564

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TFs/TRs:

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Gene ID	Type	Classification
Msa0726030	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0726030	Msa0766570	0.820314	7.322532e-53	-8.615850e-47
Msa0726030	Msa0846200	0.926995	2.174797e-91	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0726030	MtrunA17_Chr5g0417791	93.040	273	6	1	1	273	1	260	0.0	526
Msa0726030	MtrunA17_Chr4g0042561	73.518	253	56	5	2	252	8	251	7.05e-136	384
Msa0726030	MtrunA17_Chr2g0326361	66.471	170	55	1	3	170	14	183	3.59e-82	249
Msa0726030	MtrunA17_Chr7g0252971	49.632	272	110	6	3	251	8	275	1.92e-80	246
Msa0726030	MtrunA17_Chr2g0318101	65.680	169	47	3	3	161	15	182	3.58e-79	243
Msa0726030	MtrunA17_Chr7g0263931	60.417	192	72	2	1	188	9	200	3.85e-77	237
Msa0726030	MtrunA17_Chr4g0047841	61.582	177	47	2	2	157	14	190	5.39e-77	237
Msa0726030	MtrunA17_Chr8g0384721	46.970	264	114	6	1	240	5	266	5.19e-75	230
Msa0726030	MtrunA17_Chr3g0124761	59.459	185	66	4	1	185	5	180	5.57e-74	228
Msa0726030	MtrunA17_Chr8g0359921	61.875	160	59	1	1	160	12	169	3.16e-71	222

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0726030	AT1G69490.1	67.273	220	60	2	3	210	9	228	5.22e-111	321
Msa0726030	AT1G61110.1	69.325	163	41	3	2	155	15	177	6.63e-79	241
Msa0726030	AT3G04070.2	50.612	245	98	4	3	224	10	254	2.84e-77	238
Msa0726030	AT3G15510.1	62.791	172	53	3	7	168	21	191	9.05e-77	238
Msa0726030	AT3G04070.1	48.659	261	95	5	3	224	10	270	1.17e-76	237
Msa0726030	AT1G77450.1	61.212	165	62	2	1	165	8	170	1.39e-73	226
Msa0726030	AT1G01720.1	63.125	160	58	1	1	160	5	163	1.01e-72	224
Msa0726030	AT4G27410.2	50.000	218	90	4	1	203	12	225	1.75e-71	222
Msa0726030	AT3G15500.1	63.462	156	53	1	1	156	12	163	2.46e-71	222
Msa0726030	AT1G52890.1	63.057	157	54	1	1	157	12	164	4.52e-71	221

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Find 70 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATTGGTGAAATTTCCA+AGG	0.561534	5_1:-26208117	Msa0726030:CDS
AATGCAGATAAATCAGACTT+TGG	0.509380	5_1:-26208873	Msa0726030:intron
AATTTATAGATATCCACTTC+TGG	0.300371	5_1:+26209153	None:intergenic
ACAGATAAATCAATCTTTAG+TGG	0.509909	5_1:-26208750	Msa0726030:CDS
ACTTCTGGGATGATAGATGC+AGG	0.582550	5_1:+26209168	None:intergenic
AGAAAGAAAGTATCCAAATG+GGG	0.672302	5_1:-26208820	Msa0726030:CDS
AGACAAGCCAATAGACAAAT+TGG	0.241711	5_1:-26208618	Msa0726030:CDS
AGAGAAAGAAAGTATCCAAA+TGG	0.427350	5_1:-26208822	Msa0726030:CDS
ATAATTAGTTCCTCATCAGT+TGG	0.510932	5_1:+26209228	None:intergenic
ATAGACAAATTGGGTCCATG+AGG	0.667001	5_1:-26208608	Msa0726030:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAATTAGTTCCTCATCAGT+TGG	+	chr5_1:26207901-26207920	None:intergenic	30.0%
AATGCAGATAAATCAGACTT+TGG	-	chr5_1:26208253-26208272	Msa0726030:intron	30.0%
TTTGGATACTTTCTTTCTCT+TGG	+	chr5_1:26208304-26208323	None:intergenic	30.0%
TATTGGAGTTAAGAAAGCTT+TGG	-	chr5_1:26208408-26208427	Msa0726030:intron	30.0%
ATTGTCTTGTAAACCTTATC+AGG	-	chr5_1:26208717-26208736	Msa0726030:CDS	30.0%
GAAGAACATCACAAAATCAT+TGG	-	chr5_1:26208923-26208942	Msa0726030:intron	30.0%
AAGAATTGGTGAAATTTCCA+AGG	-	chr5_1:26209009-26209028	Msa0726030:intron	30.0%
TGAAACAAAATAGCAGCTTT+GGG	-	chr5_1:26209231-26209250	Msa0726030:CDS	30.0%
GAACCAAGTATTTGATCAAA+GGG	-	chr5_1:26209253-26209272	Msa0726030:CDS	30.0%
ATTCCCTTTGATCAAATACT+TGG	+	chr5_1:26209259-26209278	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	26207868	26209280	26207868	ID=Msa0726030;Name=Msa0726030
chr5_1	mRNA	26207868	26209280	26207868	ID=Msa0726030-mRNA-1;Parent=Msa0726030;Name=Msa0726030-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0.5\|0\|1\|1\|1\|3\|0\|273
chr5_1	exon	26209133	26209280	26209133	ID=Msa0726030-mRNA-1:exon:9395;Parent=Msa0726030-mRNA-1
chr5_1	exon	26208608	26208888	26208608	ID=Msa0726030-mRNA-1:exon:9394;Parent=Msa0726030-mRNA-1
chr5_1	exon	26207868	26208260	26207868	ID=Msa0726030-mRNA-1:exon:9393;Parent=Msa0726030-mRNA-1
chr5_1	CDS	26209133	26209280	26209133	ID=Msa0726030-mRNA-1:cds;Parent=Msa0726030-mRNA-1
chr5_1	CDS	26208608	26208888	26208608	ID=Msa0726030-mRNA-1:cds;Parent=Msa0726030-mRNA-1
chr5_1	CDS	26207868	26208260	26207868	ID=Msa0726030-mRNA-1:cds;Parent=Msa0726030-mRNA-1

Gene Sequence

>Msa0726030

ATGGAACTCCCACCTGGCTTTAGGTTTCATCCAACTGATGAGGAACTAATTATGTATTACCTTTGTAATCAAGCAACATCACAACCATGTCCTGCATCTATCATCCCAGAAGTGGATATCTATAAATTTGATCCATGGGAATTACCAGATAAATCAGACTTTGGAGAAAATGAATGGTATTTTTTTACTCCAAGAGAAAGAAAGTATCCAAATGGGGTGAGACCAAATAGAGCAACTGTTTCTGGTTATTGGAAAGCCACTGGAACAGATAAATCAATCTTTAGTGGGTCTAAGCATATTGGAGTTAAGAAAGCTTTGGTATTTTACAAGGGTAGGCCACCAAAGGGAATCAAGACAGATTGGATTATGCATGAATATAGGTTAATTGGATCAAGAAGACAAGCCAATAGACAAATTGGGTCCATGAGGCTAGATGATTGGGTCTTATGTAGGATCTATAAGAAGAAGAACATCACAAAATCATTGGAGACAAATGAAGACTACCCAACAAATCAAATCAATATGACACAAACAAATGATAATAGTGAACAAGAATTGGTGAAATTTCCAAGGACATGTTCCCTTACTAATCTTTTGAATATGGATTATATGGGACCAATATCACAAATATTATCAGATGGATCATATAATTCAACCTTTGAATTTCAAATTAATACAGCACATGGTGGAACAATAGACCCCTTTGTAAAACCACAGATGGTTGAAATGCCTAATAACAACACTTATGCAGCTGATTTAGAGAAGTACAATGTGAAACAAAATAGCAGCTTTGGGAACCAAGTATTTGATCAAAGGGAATAA

Protein sequence

>Msa0726030

MELPPGFRFHPTDEELIMYYLCNQATSQPCPASIIPEVDIYKFDPWELPDKSDFGENEWYFFTPRERKYPNGVRPNRATVSGYWKATGTDKSIFSGSKHIGVKKALVFYKGRPPKGIKTDWIMHEYRLIGSRRQANRQIGSMRLDDWVLCRIYKKKNITKSLETNEDYPTNQINMTQTNDNSEQELVKFPRTCSLTNLLNMDYMGPISQILSDGSYNSTFEFQINTAHGGTIDPFVKPQMVEMPNNNTYAADLEKYNVKQNSSFGNQVFDQRE*