AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0730420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0730420 MtrunA17_Chr5g0423471 62.176 193 71 2 7 199 22 212 1.61e-78 233
Msa0730420 MtrunA17_Chr5g0423421 67.949 78 25 0 115 192 1 78 1.52e-33 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0730420 AT1G68260.1 60.274 146 57 1 48 193 39 183 2.31e-62 192
Msa0730420 AT1G68280.1 59.559 136 54 1 58 193 51 185 4.15e-56 176
Msa0730420 AT1G35290.1 46.961 181 82 3 14 194 16 182 3.57e-53 169
Msa0730420 AT1G35250.1 53.676 136 62 1 59 194 48 182 9.78e-52 165
Msa0730420 AT1G35250.3 55.752 113 49 1 59 171 48 159 1.79e-40 135
Msa0730420 AT1G35250.2 54.930 71 31 1 59 129 48 117 1.22e-22 89.0

Find 40 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGAGGACCAATGAATTTC+AGG 0.170484 5_1:-39540337 None:intergenic
TTGTTGTGACTTACTCTTAG+AGG 0.306908 5_1:-39540353 None:intergenic
GTTGTTAAGGTTAGGATATC+TGG 0.308671 5_1:+39541385 Msa0730420:CDS
TGGAGACAAATTTGTTGTTA+AGG 0.346111 5_1:+39541372 Msa0730420:CDS
ACTTAGTATTAAGGAGTAAT+GGG 0.359723 5_1:-39538932 None:intergenic
CTATCGTCCTGTTAGAATTC+CGG 0.374728 5_1:+39541591 Msa0730420:CDS
AGGTGAGTTGAATGCCTTCC+TGG 0.375223 5_1:-39538994 None:intergenic
GTGCTCCTGGCTTCCAAGAT+AGG 0.377070 5_1:-39541547 None:intergenic
ACATGTTCGCGATTATGAAT+TGG 0.385221 5_1:+39539747 Msa0730420:CDS
GAGCATCAGCGTTGTGCTCC+TGG 0.390293 5_1:-39541560 None:intergenic
ACAAATTTGTTGTTAAGGTT+AGG 0.394775 5_1:+39541377 Msa0730420:CDS
GCGGAAACGACTTAGTATTA+AGG 0.403153 5_1:-39538941 None:intergenic
AGATCTGCCGGAATTCTAAC+AGG 0.406014 5_1:-39541598 None:intergenic
CTCATGAGTTGTCACCTTGT+TGG 0.415293 5_1:-39539813 None:intergenic
TTTCTCTCTCGTTGATATCA+TGG 0.431142 5_1:-39539067 None:intergenic
TGGAAATAGACCTCATTGTT+TGG 0.440558 5_1:-39541454 None:intergenic
AAACTATCCCTGAAATTCAT+TGG 0.441038 5_1:+39540329 Msa0730420:CDS
CAGAATGAATGATTTCTTTG+AGG 0.447385 5_1:+39539717 Msa0730420:intron
CTCCAACTTCTCTGATTCCC+AGG 0.453760 5_1:+39538976 Msa0730420:CDS
TAGAGGACCAATGAATTTCA+GGG 0.454045 5_1:-39540336 None:intergenic
TTCCTGGGAATCAGAGAAGT+TGG 0.483978 5_1:-39538978 None:intergenic
GACAGCCTATCTTGGAAGCC+AGG 0.495074 5_1:+39541542 Msa0730420:intron
GACTTAGTATTAAGGAGTAA+TGG 0.505746 5_1:-39538933 None:intergenic
CAGAGAACTCTCTGGAATGC+AGG 0.508261 5_1:-39539014 None:intergenic
AATGAATGATTTCTTTGAGG+TGG 0.510371 5_1:+39539720 Msa0730420:CDS
ATGATATCAACGAGAGAGAA+AGG 0.513613 5_1:+39539069 Msa0730420:CDS
GTTCTCTGTGTGAGTAATGA+TGG 0.519040 5_1:+39539029 Msa0730420:CDS
TACTCACACAGAGAACTCTC+TGG 0.535337 5_1:-39539022 None:intergenic
AACTTCTCTGATTCCCAGGA+AGG 0.561471 5_1:+39538980 Msa0730420:CDS
AACAAGCATAAACAGAATTG+TGG 0.565386 5_1:-39539786 None:intergenic
GATATCATGGAGTGGTAAAG+AGG 0.567934 5_1:-39539054 None:intergenic
GATTATGAATTGGATCAATA+CGG 0.588032 5_1:+39539757 Msa0730420:CDS
GGTGAGTTGAATGCCTTCCT+GGG 0.590146 5_1:-39538993 None:intergenic
CTCTCGTTGATATCATGGAG+TGG 0.622914 5_1:-39539062 None:intergenic
TATGCTTGTTATTGCCAACA+AGG 0.650339 5_1:+39539799 Msa0730420:CDS
CTTAGTATTAAGGAGTAATG+GGG 0.653537 5_1:-39538931 None:intergenic
TAATGGGGAAAGTAACCCCA+TGG 0.671575 5_1:-39538916 None:intergenic
TTATAAGCTACCAAACAATG+AGG 0.689144 5_1:+39541444 Msa0730420:CDS
TGAATTGGATCAATACGGTG+TGG 0.700307 5_1:+39539762 Msa0730420:CDS
GTTGGAGATTTACTGCAGAG+CGG 0.727669 5_1:-39538960 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTGAAAAATGATATT+TGG - chr5_1:39540030-39540049 None:intergenic 10.0%
!!! TTTTCAATAAATTAAACATT+TGG + chr5_1:39540038-39540057 Msa0730420:intron 10.0%
!!! TTTTTGTTAAAAATTAAAGA+AGG + chr5_1:39540619-39540638 Msa0730420:intron 10.0%
!!! TTTTGTTAAAAATTAAAGAA+GGG + chr5_1:39540620-39540639 Msa0730420:intron 10.0%
!! TAAAATATGCAAAATATATA+TGG - chr5_1:39541086-39541105 None:intergenic 10.0%
!! AATTTATAGTTTATTTAGAT+GGG - chr5_1:39541202-39541221 None:intergenic 10.0%
!!! TGAAAATATTTGTTAATATT+TGG + chr5_1:39541509-39541528 Msa0730420:intron 10.0%
!!! ATTTTTATTTTTTCCTCTTA+TGG + chr5_1:39539148-39539167 Msa0730420:intron 15.0%
!! ATAATTTACAAAGAAAAAGT+AGG + chr5_1:39539911-39539930 Msa0730420:intron 15.0%
!! TTTATATAAATATCTTCATC+AGG + chr5_1:39540227-39540246 Msa0730420:intron 15.0%
!! TTTGTTAAAAATTAAAGAAG+GGG + chr5_1:39540621-39540640 Msa0730420:intron 15.0%
!!! GATAGTTTATTTTTTTAAGA+AGG + chr5_1:39540758-39540777 Msa0730420:intron 15.0%
!!! TCAAATTTTATAAAGTACTA+AGG - chr5_1:39540981-39541000 None:intergenic 15.0%
!!! ATTTTGCATATTTTACAAAT+TGG + chr5_1:39541091-39541110 Msa0730420:intron 15.0%
!!! TAAATACTTTTTTTGACAAA+AGG - chr5_1:39541167-39541186 None:intergenic 15.0%
!! CAATTTATAGTTTATTTAGA+TGG - chr5_1:39541203-39541222 None:intergenic 15.0%
!! AAGTTGAAAACTTTAAATTG+TGG + chr5_1:39539173-39539192 Msa0730420:intron 20.0%
!! ATAACTGACATGTATATATA+AGG - chr5_1:39539281-39539300 None:intergenic 20.0%
!!! TTTTAATTGTGATGATATCT+TGG + chr5_1:39540277-39540296 Msa0730420:CDS 20.0%
!! ATTTCACAATACACTATAAA+TGG + chr5_1:39541278-39541297 Msa0730420:intron 20.0%
! ACTTAGTATTAAGGAGTAAT+GGG - chr5_1:39538935-39538954 None:intergenic 25.0%
! TGTATAATGAAGCATAAACA+AGG - chr5_1:39539119-39539138 None:intergenic 25.0%
! TGATTGATCATATATAACTC+TGG + chr5_1:39539212-39539231 Msa0730420:intron 25.0%
!! TTTAAATTTTATCCAGCTCA+TGG - chr5_1:39539490-39539509 None:intergenic 25.0%
! ATTGCAAAGTGACATAAAAT+TGG + chr5_1:39539575-39539594 Msa0730420:intron 25.0%
!!! ATGTAAACTTTTTCAACCTT+CGG + chr5_1:39539601-39539620 Msa0730420:intron 25.0%
! GATTATGAATTGGATCAATA+CGG + chr5_1:39539757-39539776 Msa0730420:CDS 25.0%
! AAATAAGCTGTCAATCAAAT+TGG - chr5_1:39539884-39539903 None:intergenic 25.0%
! AGAAAAAGTAGGTATTTGTA+AGG + chr5_1:39539922-39539941 Msa0730420:intron 25.0%
! ACTTTATCTCTCTATTGTTA+TGG - chr5_1:39539951-39539970 None:intergenic 25.0%
! AATCAAGAATGATGATTACT+TGG + chr5_1:39540093-39540112 Msa0730420:intron 25.0%
! ATCAAGAATGATGATTACTT+GGG + chr5_1:39540094-39540113 Msa0730420:intron 25.0%
!! CATCAATTCTTAAAAAGCAT+TGG + chr5_1:39540254-39540273 Msa0730420:CDS 25.0%
! TTGAAAATGACTCATTGTAA+TGG + chr5_1:39540793-39540812 Msa0730420:intron 25.0%
! TGAAAATGACTCATTGTAAT+GGG + chr5_1:39540794-39540813 Msa0730420:intron 25.0%
!! AAAGGTTTGGAATAAATACT+TGG - chr5_1:39541149-39541168 None:intergenic 25.0%
!!! ACTTTTTTTGACAAAAGGTT+TGG - chr5_1:39541162-39541181 None:intergenic 25.0%
!! TTATTTAGATGGGACAATTT+TGG - chr5_1:39541192-39541211 None:intergenic 25.0%
! ATAAATTGCCTAAAATGTCA+AGG + chr5_1:39541215-39541234 Msa0730420:intron 25.0%
!! TATGTTATCCTTGACATTTT+AGG - chr5_1:39541226-39541245 None:intergenic 25.0%
!!! ACAAATTTGTTGTTAAGGTT+AGG + chr5_1:39541377-39541396 Msa0730420:CDS 25.0%
CTTAGTATTAAGGAGTAATG+GGG - chr5_1:39538934-39538953 None:intergenic 30.0%
GACTTAGTATTAAGGAGTAA+TGG - chr5_1:39538936-39538955 None:intergenic 30.0%
TCAAATCACATTCTTACCTT+CGG - chr5_1:39539368-39539387 None:intergenic 30.0%
GAATGTTATATATGATCCGA+AGG - chr5_1:39539620-39539639 None:intergenic 30.0%
CAGAATGAATGATTTCTTTG+AGG + chr5_1:39539717-39539736 Msa0730420:intron 30.0%
! AATGAATGATTTCTTTGAGG+TGG + chr5_1:39539720-39539739 Msa0730420:CDS 30.0%
AACAAGCATAAACAGAATTG+TGG - chr5_1:39539789-39539808 None:intergenic 30.0%
TCACAAAAGTTGTCACTAAA+TGG + chr5_1:39540000-39540019 Msa0730420:intron 30.0%
AAACTATCCCTGAAATTCAT+TGG + chr5_1:39540329-39540348 Msa0730420:CDS 30.0%
!! TTTTTCAAAGCAAGAGTTAG+AGG + chr5_1:39540547-39540566 Msa0730420:intron 30.0%
AGGGGACGTATAATAAATAA+CGG + chr5_1:39540639-39540658 Msa0730420:intron 30.0%
GAAAATGACTCATTGTAATG+GGG + chr5_1:39540795-39540814 Msa0730420:intron 30.0%
!! CATGTTGCTCTTTTAAGAAA+AGG - chr5_1:39540905-39540924 None:intergenic 30.0%
! CTTTTCGCTAATGTTTCTTA+TGG + chr5_1:39541326-39541345 Msa0730420:intron 30.0%
! TTTAAAACTTGCAACCAGAA+TGG + chr5_1:39541352-39541371 Msa0730420:intron 30.0%
!! TGGAGACAAATTTGTTGTTA+AGG + chr5_1:39541372-39541391 Msa0730420:CDS 30.0%
TTATAAGCTACCAAACAATG+AGG + chr5_1:39541444-39541463 Msa0730420:CDS 30.0%
CGAAAGTAAATAGTAAGTGA+TGG - chr5_1:39541477-39541496 None:intergenic 30.0%
! ATTTTAGACTTAAGATCTGC+CGG - chr5_1:39541613-39541632 None:intergenic 30.0%
TTTCTCTCTCGTTGATATCA+TGG - chr5_1:39539070-39539089 None:intergenic 35.0%
ATGATATCAACGAGAGAGAA+AGG + chr5_1:39539069-39539088 Msa0730420:CDS 35.0%
GAAGCATAAACAAGGTTTGT+GGG - chr5_1:39539111-39539130 None:intergenic 35.0%
TGAAGCATAAACAAGGTTTG+TGG - chr5_1:39539112-39539131 None:intergenic 35.0%
! GTTTTCAACTTCACCATAAG+AGG - chr5_1:39539164-39539183 None:intergenic 35.0%
AAATCACATTCTTACCTTCG+GGG - chr5_1:39539366-39539385 None:intergenic 35.0%
CAAATCACATTCTTACCTTC+GGG - chr5_1:39539367-39539386 None:intergenic 35.0%
ACATGTTCGCGATTATGAAT+TGG + chr5_1:39539747-39539766 Msa0730420:CDS 35.0%
!! TATGCTTGTTATTGCCAACA+AGG + chr5_1:39539799-39539818 Msa0730420:CDS 35.0%
ACAACTCATGAGTTACTTCT+TGG + chr5_1:39539824-39539843 Msa0730420:intron 35.0%
!! TGAACACAAGTTTTATGCGA+CGG - chr5_1:39540072-39540091 None:intergenic 35.0%
!!! TTTCAGGGATAGTTTTGACA+TGG - chr5_1:39540324-39540343 None:intergenic 35.0%
TAGAGGACCAATGAATTTCA+GGG - chr5_1:39540339-39540358 None:intergenic 35.0%
TTAGAGGACCAATGAATTTC+AGG - chr5_1:39540340-39540359 None:intergenic 35.0%
TTGTTGTGACTTACTCTTAG+AGG - chr5_1:39540356-39540375 None:intergenic 35.0%
GTAAGTCACAACAAAATCCT+TGG + chr5_1:39540362-39540381 Msa0730420:intron 35.0%
CTAGAAAACAAAAACGACCA+AGG - chr5_1:39540382-39540401 None:intergenic 35.0%
!!! CTTGGTCGTTTTTGTTTTCT+AGG + chr5_1:39540380-39540399 Msa0730420:intron 35.0%
GGACGTATAATAAATAACGG+AGG + chr5_1:39540642-39540661 Msa0730420:intron 35.0%
AAATGGCTTCTGTAACTTTC+AGG - chr5_1:39540700-39540719 None:intergenic 35.0%
GTCAGTTAAGCTACATGAAA+TGG - chr5_1:39540717-39540736 None:intergenic 35.0%
AATGTGTACTAGTGTGAACA+TGG - chr5_1:39541060-39541079 None:intergenic 35.0%
CAACAAATTTGTCTCCATTC+TGG - chr5_1:39541369-39541388 None:intergenic 35.0%
GTTGTTAAGGTTAGGATATC+TGG + chr5_1:39541385-39541404 Msa0730420:CDS 35.0%
TGGAAATAGACCTCATTGTT+TGG - chr5_1:39541457-39541476 None:intergenic 35.0%
!! ATTTTGATGACAGCCTATCT+TGG + chr5_1:39541534-39541553 Msa0730420:intron 35.0%
GCGGAAACGACTTAGTATTA+AGG - chr5_1:39538944-39538963 None:intergenic 40.0%
GTTCTCTGTGTGAGTAATGA+TGG + chr5_1:39539029-39539048 Msa0730420:CDS 40.0%
GATATCATGGAGTGGTAAAG+AGG - chr5_1:39539057-39539076 None:intergenic 40.0%
TGAATTGGATCAATACGGTG+TGG + chr5_1:39539762-39539781 Msa0730420:CDS 40.0%
TTGGGACACATTATGCTCAA+AGG + chr5_1:39540112-39540131 Msa0730420:intron 40.0%
CTTTCAGGAACATATGCCAA+CGG - chr5_1:39540685-39540704 None:intergenic 40.0%
CTATCGTCCTGTTAGAATTC+CGG + chr5_1:39541591-39541610 Msa0730420:CDS 40.0%
GTTGGAGATTTACTGCAGAG+CGG - chr5_1:39538963-39538982 None:intergenic 45.0%
TTCCTGGGAATCAGAGAAGT+TGG - chr5_1:39538981-39539000 None:intergenic 45.0%
AACTTCTCTGATTCCCAGGA+AGG + chr5_1:39538980-39538999 Msa0730420:CDS 45.0%
TACTCACACAGAGAACTCTC+TGG - chr5_1:39539025-39539044 None:intergenic 45.0%
CTCTCGTTGATATCATGGAG+TGG - chr5_1:39539065-39539084 None:intergenic 45.0%
AAGATACTTAGCTTCCCCGA+AGG + chr5_1:39539349-39539368 Msa0730420:intron 45.0%
CTCATGAGTTGTCACCTTGT+TGG - chr5_1:39539816-39539835 None:intergenic 45.0%
GACTAGAGACTTACAACCGT+TGG + chr5_1:39540666-39540685 Msa0730420:intron 45.0%
AGATCTGCCGGAATTCTAAC+AGG - chr5_1:39541601-39541620 None:intergenic 45.0%
!!! TTTTTAAATAATAAAAACTA+AGG + chr5_1:39540570-39540589 Msa0730420:intron 5.0%
CTCCAACTTCTCTGATTCCC+AGG + chr5_1:39538976-39538995 Msa0730420:CDS 50.0%
! GGTGAGTTGAATGCCTTCCT+GGG - chr5_1:39538996-39539015 None:intergenic 50.0%
! AGGTGAGTTGAATGCCTTCC+TGG - chr5_1:39538997-39539016 None:intergenic 50.0%
!! CAGAGAACTCTCTGGAATGC+AGG - chr5_1:39539017-39539036 None:intergenic 50.0%
CATGGTGTCCTTATGGGTCA+CGG - chr5_1:39539472-39539491 None:intergenic 50.0%
CCAGCTCATGGTGTCCTTAT+GGG - chr5_1:39539478-39539497 None:intergenic 50.0%
TCCAGCTCATGGTGTCCTTA+TGG - chr5_1:39539479-39539498 None:intergenic 50.0%
ATGGGTCACGGTGACTCGAT+AGG - chr5_1:39539460-39539479 None:intergenic 55.0%
TCGAGTCACCGTGACCCATA+AGG + chr5_1:39539461-39539480 Msa0730420:intron 55.0%
CCCATAAGGACACCATGAGC+TGG + chr5_1:39539475-39539494 Msa0730420:intron 55.0%
GACAGCCTATCTTGGAAGCC+AGG + chr5_1:39541542-39541561 Msa0730420:intron 55.0%
GTGCTCCTGGCTTCCAAGAT+AGG - chr5_1:39541550-39541569 None:intergenic 55.0%
GAGCATCAGCGTTGTGCTCC+TGG - chr5_1:39541563-39541582 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 39538918 39541663 39538918 ID=Msa0730420;Name=Msa0730420
chr5_1 mRNA 39538918 39541663 39538918 ID=Msa0730420-mRNA-1;Parent=Msa0730420;Name=Msa0730420-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|1|1|5|0|199
chr5_1 exon 39538918 39539090 39538918 ID=Msa0730420-mRNA-1:exon:11676;Parent=Msa0730420-mRNA-1
chr5_1 exon 39539720 39539820 39539720 ID=Msa0730420-mRNA-1:exon:11677;Parent=Msa0730420-mRNA-1
chr5_1 exon 39540249 39540361 39540249 ID=Msa0730420-mRNA-1:exon:11678;Parent=Msa0730420-mRNA-1
chr5_1 exon 39541370 39541465 39541370 ID=Msa0730420-mRNA-1:exon:11679;Parent=Msa0730420-mRNA-1
chr5_1 exon 39541547 39541663 39541547 ID=Msa0730420-mRNA-1:exon:11680;Parent=Msa0730420-mRNA-1
chr5_1 CDS 39538918 39539090 39538918 ID=Msa0730420-mRNA-1:cds;Parent=Msa0730420-mRNA-1
chr5_1 CDS 39539720 39539820 39539720 ID=Msa0730420-mRNA-1:cds;Parent=Msa0730420-mRNA-1
chr5_1 CDS 39540249 39540361 39540249 ID=Msa0730420-mRNA-1:cds;Parent=Msa0730420-mRNA-1
chr5_1 CDS 39541370 39541465 39541370 ID=Msa0730420-mRNA-1:cds;Parent=Msa0730420-mRNA-1
chr5_1 CDS 39541547 39541663 39541547 ID=Msa0730420-mRNA-1:cds;Parent=Msa0730420-mRNA-1
Gene Sequence

>Msa0730420

ATGGGGTTACTTTCCCCATTACTCCTTAATACTAAGTCGTTTCCGCTCTGCAGTAAATCTCCAACTTCTCTGATTCCCAGGAAGGCATTCAACTCACCTGCATTCCAGAGAGTTCTCTGTGTGAGTAATGATGGTTCCTCTTTACCACTCCATGATATCAACGAGAGAGAAAGAATGAATGATTTCTTTGAGGTGGAACTACATGTTCGCGATTATGAATTGGATCAATACGGTGTGGTCCACAATTCTGTTTATGCTTGTTATTGCCAACAAGGTCGTCATCAATTCTTAAAAAGCATTGGTTTTAATTGTGATGATATCTTGGATGCTAGAGATTATGCGTTTGCCATGTCAAAACTATCCCTGAAATTCATTGGTCCTCTAAGAAATGGAGACAAATTTGTTGTTAAGGTTAGGATATCTGGATTTTCAGCTGCTCGAGTTTACTTTGATCATTTCATTTATAAGCTACCAAACAATGAGCCTATCTTGGAAGCCAGGAGCACAACGCTGATGCTCAATAAAAACTATCGTCCTGTTAGAATTCCGGCAGATCTTAAGTCTAAAATTGCAAAATTTTCTTATAGCAGTGAGTCTTAA

Protein sequence

>Msa0730420

MGLLSPLLLNTKSFPLCSKSPTSLIPRKAFNSPAFQRVLCVSNDGSSLPLHDINERERMNDFFEVELHVRDYELDQYGVVHNSVYACYCQQGRHQFLKSIGFNCDDILDARDYAFAMSKLSLKFIGPLRNGDKFVVKVRISGFSAARVYFDHFIYKLPNNEPILEARSTTLMLNKNYRPVRIPADLKSKIAKFSYSSES*