AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999340 MtrunA17_Chr6g0485481 52.239 67 32 0 19 85 23 89 1.54e-19 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGAAGACATTGATTAT+TGG 0.204699 6_4:+81296632 Msa0999340:intron
AATCAAGTCCTTCATCGTTT+AGG 0.330565 6_4:-81296660 None:intergenic
GAGTTCCACCGGCTGCAATA+AGG 0.392268 6_4:-81296807 None:intergenic
GTTAATTCGATTATTAAAGC+CGG 0.475431 6_4:+81296723 Msa0999340:CDS
TTGGAAATTATCAAGAAGAA+TGG 0.513592 6_4:+81296776 Msa0999340:CDS
TTAATTCGATTATTAAAGCC+GGG 0.522204 6_4:+81296724 Msa0999340:CDS
CAAAGTTACCTTATTGCAGC+CGG 0.529219 6_4:+81296799 Msa0999340:CDS
ACCATGTAGAGGAGTTCAAT+CGG 0.544476 6_4:+81296026 Msa0999340:CDS
CTGTAAAAGTTAACATGTAG+AGG 0.559805 6_4:-81296829 None:intergenic
GCCGATTGAACTCCTCTACA+TGG 0.561743 6_4:-81296027 None:intergenic
TGGCAAGACCTAAACGATGA+AGG 0.570614 6_4:+81296652 Msa0999340:CDS
AGTTACCTTATTGCAGCCGG+TGG 0.602448 6_4:+81296802 Msa0999340:CDS
AACATGTAGAGGAGTTCCAC+CGG 0.612575 6_4:-81296818 None:intergenic
TTGTAAAAGTTACCATGTAG+AGG 0.612953 6_4:+81296015 Msa0999340:CDS
GAGTTCAATCGGCTGCAACA+AGG 0.644422 6_4:+81296037 Msa0999340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTTTGAAAAAAAAATT+AGG + chr6_4:81296458-81296477 Msa0999340:intron 10.0%
!!! AATGTTTTGAAAAAAAAATT+AGG + chr6_4:81296458-81296477 Msa0999340:intron 10.0%
!!! TAAAAAATTAAAGTATTACC+CGG - chr6_4:81296745-81296764 None:intergenic 15.0%
!!! TAAAAAATTAAAGTATTACC+CGG - chr6_4:81296745-81296764 None:intergenic 15.0%
!!! TTTAGTTTTTTTTACTTCAG+CGG - chr6_4:81296096-81296115 None:intergenic 20.0%
!! TTAATTAGAATATCAAAGTC+GGG - chr6_4:81296125-81296144 None:intergenic 20.0%
!! GTTAATTAGAATATCAAAGT+CGG - chr6_4:81296126-81296145 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG - chr6_4:81296632-81296651 None:intergenic 20.0%
!!! GTCTTCATTTTTAAATTCAA+AGG - chr6_4:81296623-81296642 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG + chr6_4:81296632-81296651 Msa0999340:intron 20.0%
!!! ACTTTAATTTTTTACTTCAG+TGG + chr6_4:81296751-81296770 Msa0999340:CDS 20.0%
!!! TTTAGTTTTTTTTACTTCAG+CGG - chr6_4:81296096-81296115 None:intergenic 20.0%
!! TTAATTAGAATATCAAAGTC+GGG - chr6_4:81296125-81296144 None:intergenic 20.0%
!! GTTAATTAGAATATCAAAGT+CGG - chr6_4:81296126-81296145 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG - chr6_4:81296632-81296651 None:intergenic 20.0%
!!! GTCTTCATTTTTAAATTCAA+AGG - chr6_4:81296623-81296642 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG + chr6_4:81296632-81296651 Msa0999340:intron 20.0%
!!! ACTTTAATTTTTTACTTCAG+TGG + chr6_4:81296751-81296770 Msa0999340:CDS 20.0%
! TTAGCATCAATACAAAAATC+AGG + chr6_4:81296170-81296189 Msa0999340:intron 25.0%
!! AGAGAATTGCTAATAACTTT+TGG - chr6_4:81296245-81296264 None:intergenic 25.0%
! GAAAAAAAAATTAGGCGTTA+CGG + chr6_4:81296466-81296485 Msa0999340:intron 25.0%
! GTTAATTCGATTATTAAAGC+CGG + chr6_4:81296723-81296742 Msa0999340:CDS 25.0%
! TTAATTCGATTATTAAAGCC+GGG + chr6_4:81296724-81296743 Msa0999340:CDS 25.0%
!!! ATTTTTTACTTCAGTGGAAT+TGG + chr6_4:81296757-81296776 Msa0999340:CDS 25.0%
! TTGGAAATTATCAAGAAGAA+TGG + chr6_4:81296776-81296795 Msa0999340:CDS 25.0%
! TTAGCATCAATACAAAAATC+AGG + chr6_4:81296170-81296189 Msa0999340:intron 25.0%
!! AGAGAATTGCTAATAACTTT+TGG - chr6_4:81296245-81296264 None:intergenic 25.0%
! GAAAAAAAAATTAGGCGTTA+CGG + chr6_4:81296466-81296485 Msa0999340:intron 25.0%
! GTTAATTCGATTATTAAAGC+CGG + chr6_4:81296723-81296742 Msa0999340:CDS 25.0%
! TTAATTCGATTATTAAAGCC+GGG + chr6_4:81296724-81296743 Msa0999340:CDS 25.0%
!!! ATTTTTTACTTCAGTGGAAT+TGG + chr6_4:81296757-81296776 Msa0999340:CDS 25.0%
! TTGGAAATTATCAAGAAGAA+TGG + chr6_4:81296776-81296795 Msa0999340:CDS 25.0%
TTGTAAAAGTTACCATGTAG+AGG + chr6_4:81296015-81296034 Msa0999340:CDS 30.0%
CATGGAAATTATCAAGAAGA+AGG - chr6_4:81296072-81296091 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG + chr6_4:81296253-81296272 Msa0999340:intron 30.0%
TCGGAATATGTGTTAATTGT+AGG + chr6_4:81296272-81296291 Msa0999340:intron 30.0%
ATGTGTTAATTGTAGGTTCT+CGG + chr6_4:81296279-81296298 Msa0999340:intron 30.0%
AGGTGTTAGTTGTAAGTTAT+CGG - chr6_4:81296570-81296589 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG - chr6_4:81296253-81296272 None:intergenic 30.0%
CTGTAAAAGTTAACATGTAG+AGG - chr6_4:81296832-81296851 None:intergenic 30.0%
TTGTAAAAGTTACCATGTAG+AGG + chr6_4:81296015-81296034 Msa0999340:CDS 30.0%
CATGGAAATTATCAAGAAGA+AGG - chr6_4:81296072-81296091 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG + chr6_4:81296253-81296272 Msa0999340:intron 30.0%
TCGGAATATGTGTTAATTGT+AGG + chr6_4:81296272-81296291 Msa0999340:intron 30.0%
ATGTGTTAATTGTAGGTTCT+CGG + chr6_4:81296279-81296298 Msa0999340:intron 30.0%
AGGTGTTAGTTGTAAGTTAT+CGG - chr6_4:81296570-81296589 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG - chr6_4:81296253-81296272 None:intergenic 30.0%
CTGTAAAAGTTAACATGTAG+AGG - chr6_4:81296832-81296851 None:intergenic 30.0%
!!! TTTTTTTACTTCAGCGGACA+TGG - chr6_4:81296090-81296109 None:intergenic 35.0%
TTCTCTTATCTTCTCGGAAT+AGG - chr6_4:81296259-81296278 None:intergenic 35.0%
AATCAAGTCCTTCATCGTTT+AGG - chr6_4:81296663-81296682 None:intergenic 35.0%
!!! TTTTTTTACTTCAGCGGACA+TGG - chr6_4:81296090-81296109 None:intergenic 35.0%
TTCTCTTATCTTCTCGGAAT+AGG - chr6_4:81296259-81296278 None:intergenic 35.0%
AATCAAGTCCTTCATCGTTT+AGG - chr6_4:81296663-81296682 None:intergenic 35.0%
ACCATGTAGAGGAGTTCAAT+CGG + chr6_4:81296026-81296045 Msa0999340:CDS 40.0%
! TAGGCGTTACGGACAAATTT+TGG + chr6_4:81296477-81296496 Msa0999340:intron 40.0%
CAAAGTTACCTTATTGCAGC+CGG + chr6_4:81296799-81296818 Msa0999340:CDS 40.0%
ACCATGTAGAGGAGTTCAAT+CGG + chr6_4:81296026-81296045 Msa0999340:CDS 40.0%
! TAGGCGTTACGGACAAATTT+TGG + chr6_4:81296477-81296496 Msa0999340:intron 40.0%
CAAAGTTACCTTATTGCAGC+CGG + chr6_4:81296799-81296818 Msa0999340:CDS 40.0%
TGGCAAGACAGAAACGATGA+AGG - chr6_4:81296197-81296216 None:intergenic 45.0%
TGGCAAGACCTAAACGATGA+AGG + chr6_4:81296652-81296671 Msa0999340:CDS 45.0%
AACATGTAGAGGAGTTCCAC+CGG - chr6_4:81296821-81296840 None:intergenic 45.0%
TGGCAAGACAGAAACGATGA+AGG - chr6_4:81296197-81296216 None:intergenic 45.0%
TGGCAAGACCTAAACGATGA+AGG + chr6_4:81296652-81296671 Msa0999340:CDS 45.0%
AACATGTAGAGGAGTTCCAC+CGG - chr6_4:81296821-81296840 None:intergenic 45.0%
GCCGATTGAACTCCTCTACA+TGG - chr6_4:81296030-81296049 None:intergenic 50.0%
GAGTTCAATCGGCTGCAACA+AGG + chr6_4:81296037-81296056 Msa0999340:CDS 50.0%
AGTTACCTTATTGCAGCCGG+TGG + chr6_4:81296802-81296821 Msa0999340:CDS 50.0%
GCCGATTGAACTCCTCTACA+TGG - chr6_4:81296030-81296049 None:intergenic 50.0%
GAGTTCAATCGGCTGCAACA+AGG + chr6_4:81296037-81296056 Msa0999340:CDS 50.0%
AGTTACCTTATTGCAGCCGG+TGG + chr6_4:81296802-81296821 Msa0999340:CDS 50.0%
! GAGTTCCACCGGCTGCAATA+AGG - chr6_4:81296810-81296829 None:intergenic 55.0%
! GAGTTCCACCGGCTGCAATA+AGG - chr6_4:81296810-81296829 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81296001 81296891 81296001 ID=Msa0999340;Name=Msa0999340
chr6_4 mRNA 81296001 81296891 81296001 ID=Msa0999340-mRNA-1;Parent=Msa0999340;Name=Msa0999340-mRNA-1;_AED=0.52;_eAED=0.62;_QI=0|0|0|0.5|0|0|2|0|102
chr6_4 exon 81296001 81296058 81296001 ID=Msa0999340-mRNA-1:exon:15554;Parent=Msa0999340-mRNA-1
chr6_4 exon 81296641 81296891 81296641 ID=Msa0999340-mRNA-1:exon:15555;Parent=Msa0999340-mRNA-1
chr6_4 CDS 81296001 81296058 81296001 ID=Msa0999340-mRNA-1:cds;Parent=Msa0999340-mRNA-1
chr6_4 CDS 81296641 81296891 81296641 ID=Msa0999340-mRNA-1:cds;Parent=Msa0999340-mRNA-1
Gene Sequence

>Msa0999340

ATGAATGAGATGTATTGTAAAAGTTACCATGTAGAGGAGTTCAATCGGCTGCAACAAGACATTGATTATTGGCAAGACCTAAACGATGAAGGACTTGATTTTCGTATTGATGTTAAGAAAATATATGAAAGATTAACTCAGTTAATTCGATTATTAAAGCCGGGTAATACTTTAATTTTTTACTTCAGTGGAATTGGAAATTATCAAGAAGAATGGCAAAGTTACCTTATTGCAGCCGGTGGAACTCCTCTACATGTTAACTTTTACAGCACGTCTCATGCATATATTAATTTTATTTTCAACTCTTAA

Protein sequence

>Msa0999340

MNEMYCKSYHVEEFNRLQQDIDYWQDLNDEGLDFRIDVKKIYERLTQLIRLLKPGNTLIFYFSGIGNYQEEWQSYLIAAGGTPLHVNFYSTSHAYINFIFNS*