Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0732260 | Msa1082500 | 0.802765 | 4.803901e-49 | -8.615850e-47 |
| Msa0484780 | Msa0732260 | 0.802752 | 4.832702e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACTCTCAAATGTGTAAT+TGG | 0.302959 | 5_1:-44422082 | None:intergenic |
| ATTTGAGAGTTTCTCCTTCA+AGG | 0.366941 | 5_1:+44422092 | Msa0732260:CDS |
| AGTTCACTATGATACAAAAC+TGG | 0.396308 | 5_1:+44421878 | Msa0732260:CDS |
| CTTAATTCAGCACTATGAAA+TGG | 0.424703 | 5_1:-44422287 | None:intergenic |
| CAGAGATGAAACAATAAGTA+AGG | 0.436163 | 5_1:+44421846 | Msa0732260:CDS |
| GAATTAATTAGATACCTTGA+AGG | 0.547758 | 5_1:-44422106 | None:intergenic |
| GATGAAACAATAAGTAAGGG+TGG | 0.583084 | 5_1:+44421850 | Msa0732260:CDS |
| ACCTCTTCTTAATCTTAGTG+TGG | 0.594031 | 5_1:+44422047 | Msa0732260:CDS |
| AGAGATGAAACAATAAGTAA+GGG | 0.609349 | 5_1:+44421847 | Msa0732260:CDS |
| TCCACACTAAGATTAAGAAG+AGG | 0.651547 | 5_1:-44422048 | None:intergenic |
| AAACTGGAACAGTTATGATG+AGG | 0.665748 | 5_1:+44421894 | Msa0732260:CDS |
| GATATGTTAGGATCAATGGA+AGG | 0.689242 | 5_1:+44421820 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAAATGAATATTCTTTAA+AGG | - | chr5_1:44422234-44422253 | None:intergenic | 15.0% |
| !! | GAGATTGAAATTAAATAAGA+AGG | - | chr5_1:44422199-44422218 | None:intergenic | 20.0% |
| ! | AGAGATGAAACAATAAGTAA+GGG | + | chr5_1:44421847-44421866 | Msa0732260:CDS | 25.0% |
| ! | TTTGACGGTTAAGATTAAAT+AGG | + | chr5_1:44421954-44421973 | Msa0732260:intron | 25.0% |
| ! | AAAATCAAAAGTCAATCTCA+TGG | - | chr5_1:44422008-44422027 | None:intergenic | 25.0% |
| ! | GAATTAATTAGATACCTTGA+AGG | - | chr5_1:44422109-44422128 | None:intergenic | 25.0% |
| !! | TCTTTAAAGGAATTACAGTA+GGG | - | chr5_1:44422221-44422240 | None:intergenic | 25.0% |
| !! | TTCTTTAAAGGAATTACAGT+AGG | - | chr5_1:44422222-44422241 | None:intergenic | 25.0% |
| CAGAGATGAAACAATAAGTA+AGG | + | chr5_1:44421846-44421865 | Msa0732260:CDS | 30.0% | |
| AGTTCACTATGATACAAAAC+TGG | + | chr5_1:44421878-44421897 | Msa0732260:CDS | 30.0% | |
| GAAACTCTCAAATGTGTAAT+TGG | - | chr5_1:44422085-44422104 | None:intergenic | 30.0% | |
| AGAAAACATAGTCTAACACA+TGG | - | chr5_1:44422150-44422169 | None:intergenic | 30.0% | |
| GATGAAACAATAAGTAAGGG+TGG | + | chr5_1:44421850-44421869 | Msa0732260:CDS | 35.0% | |
| AAACTGGAACAGTTATGATG+AGG | + | chr5_1:44421894-44421913 | Msa0732260:CDS | 35.0% | |
| AACAGTTATGATGAGGTACA+TGG | + | chr5_1:44421901-44421920 | Msa0732260:intron | 35.0% | |
| TATTCCTTTCCTGCATTTGA+CGG | + | chr5_1:44421939-44421958 | Msa0732260:intron | 35.0% | |
| TAATCTTAACCGTCAAATGC+AGG | - | chr5_1:44421951-44421970 | None:intergenic | 35.0% | |
| ACCTCTTCTTAATCTTAGTG+TGG | + | chr5_1:44422047-44422066 | Msa0732260:CDS | 35.0% | |
| TCCACACTAAGATTAAGAAG+AGG | - | chr5_1:44422051-44422070 | None:intergenic | 35.0% | |
| ATTTGAGAGTTTCTCCTTCA+AGG | + | chr5_1:44422092-44422111 | Msa0732260:CDS | 35.0% | |
| ! | TAGACTATGTTTTCTCAGCT+AGG | + | chr5_1:44422155-44422174 | Msa0732260:intron | 35.0% |
| TTAACCGTCAAATGCAGGAA+AGG | - | chr5_1:44421946-44421965 | None:intergenic | 40.0% | |
| TCTAACACATGGCTGAAGAT+AGG | - | chr5_1:44422139-44422158 | None:intergenic | 40.0% | |
| CACATGGCTGAAGATAGGAA+GGG | - | chr5_1:44422134-44422153 | None:intergenic | 45.0% | |
| ACACATGGCTGAAGATAGGA+AGG | - | chr5_1:44422135-44422154 | None:intergenic | 45.0% | |
| !!! | TGATTTTTTTTTTTTTAAAT+AGG | + | chr5_1:44422021-44422040 | Msa0732260:intron | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 44421835 | 44422297 | 44421835 | ID=Msa0732260;Name=Msa0732260 |
| chr5_1 | mRNA | 44421835 | 44422297 | 44421835 | ID=Msa0732260-mRNA-1;Parent=Msa0732260;Name=Msa0732260-mRNA-1;_AED=0.71;_eAED=0.71;_QI=0|0|0.33|0.33|1|1|3|0|54 |
| chr5_1 | exon | 44421835 | 44421915 | 44421835 | ID=Msa0732260-mRNA-1:exon:12626;Parent=Msa0732260-mRNA-1 |
| chr5_1 | exon | 44422043 | 44422113 | 44422043 | ID=Msa0732260-mRNA-1:exon:12627;Parent=Msa0732260-mRNA-1 |
| chr5_1 | exon | 44422285 | 44422297 | 44422285 | ID=Msa0732260-mRNA-1:exon:12628;Parent=Msa0732260-mRNA-1 |
| chr5_1 | CDS | 44421835 | 44421915 | 44421835 | ID=Msa0732260-mRNA-1:cds;Parent=Msa0732260-mRNA-1 |
| chr5_1 | CDS | 44422043 | 44422113 | 44422043 | ID=Msa0732260-mRNA-1:cds;Parent=Msa0732260-mRNA-1 |
| chr5_1 | CDS | 44422285 | 44422297 | 44422285 | ID=Msa0732260-mRNA-1:cds;Parent=Msa0732260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
