Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0733070 | XP_003615387.1 | 96.774 | 124 | 4 | 0 | 1 | 124 | 1 | 124 | 8.42e-83 | 248 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0733070 | sp|Q9LSK9|Y5566_ARATH | 40.909 | 110 | 60 | 2 | 8 | 117 | 14 | 118 | 3.18e-22 | 88.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0733070 | B7FMD8 | 96.774 | 124 | 4 | 0 | 1 | 124 | 1 | 124 | 4.02e-83 | 248 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0450400 | Msa0733070 | -0.812498 | 4.113284e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0733070 | MtrunA17_Chr5g0428121 | 96.774 | 124 | 4 | 0 | 1 | 124 | 1 | 124 | 7.74e-87 | 248 |
| Msa0733070 | MtrunA17_Chr3g0141081 | 57.009 | 107 | 40 | 2 | 1 | 105 | 1 | 103 | 4.70e-23 | 86.7 |
| Msa0733070 | MtrunA17_Chr8g0366891 | 35.200 | 125 | 65 | 3 | 1 | 118 | 1 | 116 | 2.83e-19 | 77.4 |
| Msa0733070 | MtrunA17_Chr5g0411511 | 37.273 | 110 | 66 | 2 | 9 | 118 | 17 | 123 | 4.45e-15 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0733070 | AT5G65660.1 | 40.909 | 110 | 60 | 2 | 8 | 117 | 14 | 118 | 3.23e-23 | 88.2 |
Find 21 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCCATGGCTATGAATTT+TGG | 0.222092 | 5_1:-46033990 | None:intergenic |
| TTAATGACCCCAGAAGAATA+TGG | 0.406197 | 5_1:-46033244 | None:intergenic |
| TTGTTTCTTCCTTGTGCACA+TGG | 0.427741 | 5_1:-46034049 | None:intergenic |
| ATAACCATTGGTCTTCCATT+GGG | 0.430090 | 5_1:+46033136 | Msa0733070:CDS |
| AATAACCATTGGTCTTCCAT+TGG | 0.431356 | 5_1:+46033135 | Msa0733070:CDS |
| GTGATACTTTCATCTGTTGG+AGG | 0.442771 | 5_1:-46034023 | None:intergenic |
| TCATCTGTTGGAGGTTTACA+TGG | 0.459367 | 5_1:-46034014 | None:intergenic |
| GGGAAGATAATTACCACAAA+AGG | 0.461341 | 5_1:-46033322 | None:intergenic |
| ATTACCACAAAAGGAAATGC+TGG | 0.491723 | 5_1:-46033313 | None:intergenic |
| ATTGTGATACTTTCATCTGT+TGG | 0.494711 | 5_1:-46034026 | None:intergenic |
| AGCTTGCCTGTGTTGATGCC+AGG | 0.530092 | 5_1:+46033957 | Msa0733070:CDS |
| AAAGGAAATGCTGGTTTCTG+AGG | 0.551783 | 5_1:-46033304 | None:intergenic |
| TTTGTTTCCATATTCTTCTG+GGG | 0.556303 | 5_1:+46033237 | Msa0733070:CDS |
| GCAGCAACTAAAATAACCAT+TGG | 0.572179 | 5_1:+46033124 | Msa0733070:CDS |
| GATCCCAAAATTCATAGCCA+TGG | 0.591806 | 5_1:+46033986 | Msa0733070:CDS |
| TATCACAATCCATGTGCACA+AGG | 0.596792 | 5_1:+46034040 | Msa0733070:CDS |
| AGGTTTACATGGACATGCCA+TGG | 0.597625 | 5_1:-46034003 | None:intergenic |
| TCATCTCCTGGCATCAACAC+AGG | 0.605248 | 5_1:-46033963 | None:intergenic |
| AAGAGAAGTGCTAAACCCAA+TGG | 0.608171 | 5_1:-46033151 | None:intergenic |
| AAATGCTGGTTTCTGAGGAG+AGG | 0.637186 | 5_1:-46033299 | None:intergenic |
| TAAACCCAATGGAAGACCAA+TGG | 0.650172 | 5_1:-46033140 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAGTACTAAATAATTTTTT+TGG | + | chr5_1:46033385-46033404 | Msa0733070:intron | 15.0% |
| !! | TAACAAAAAAAAACTAGTTA+AGG | - | chr5_1:46033554-46033573 | None:intergenic | 15.0% |
| !! | TATATATATACACACATAAT+TGG | + | chr5_1:46033866-46033885 | Msa0733070:intron | 15.0% |
| !!! | GTAGTACTAAATAATTTTTT+TGG | + | chr5_1:46033385-46033404 | Msa0733070:intron | 15.0% |
| !! | TAACAAAAAAAAACTAGTTA+AGG | - | chr5_1:46033554-46033573 | None:intergenic | 15.0% |
| !! | TATATATATACACACATAAT+TGG | + | chr5_1:46033866-46033885 | Msa0733070:intron | 15.0% |
| !! | AATTTAGTGAGAAATAACAT+GGG | - | chr5_1:46033345-46033364 | None:intergenic | 20.0% |
| !! | AAATTTAGTGAGAAATAACA+TGG | - | chr5_1:46033346-46033365 | None:intergenic | 20.0% |
| !! | AAAAAAAAACTAGTTAAGGA+AGG | - | chr5_1:46033550-46033569 | None:intergenic | 20.0% |
| !! | ACAAAATTACACAGAATAAA+AGG | + | chr5_1:46033763-46033782 | Msa0733070:intron | 20.0% |
| !! | CAAAATTACACAGAATAAAA+GGG | + | chr5_1:46033764-46033783 | Msa0733070:intron | 20.0% |
| !!! | AGATTGATTGTATTGTTTTT+TGG | + | chr5_1:46033903-46033922 | Msa0733070:intron | 20.0% |
| !! | AATTTAGTGAGAAATAACAT+GGG | - | chr5_1:46033345-46033364 | None:intergenic | 20.0% |
| !! | AAATTTAGTGAGAAATAACA+TGG | - | chr5_1:46033346-46033365 | None:intergenic | 20.0% |
| !! | AAAAAAAAACTAGTTAAGGA+AGG | - | chr5_1:46033550-46033569 | None:intergenic | 20.0% |
| !! | ACAAAATTACACAGAATAAA+AGG | + | chr5_1:46033763-46033782 | Msa0733070:intron | 20.0% |
| !! | CAAAATTACACAGAATAAAA+GGG | + | chr5_1:46033764-46033783 | Msa0733070:intron | 20.0% |
| !!! | AGATTGATTGTATTGTTTTT+TGG | + | chr5_1:46033903-46033922 | Msa0733070:intron | 20.0% |
| !!! | TCTTTTGTTGCTTATTACAT+TGG | + | chr5_1:46033200-46033219 | Msa0733070:CDS | 25.0% |
| !!! | TTTTTGTTTCCATATTCTTC+TGG | + | chr5_1:46033235-46033254 | Msa0733070:CDS | 25.0% |
| !!! | TTTTGTTTCCATATTCTTCT+GGG | + | chr5_1:46033236-46033255 | Msa0733070:CDS | 25.0% |
| ! | ATATATACACACATAATTGG+AGG | + | chr5_1:46033869-46033888 | Msa0733070:intron | 25.0% |
| !!! | TCTTTTGTTGCTTATTACAT+TGG | + | chr5_1:46033200-46033219 | Msa0733070:CDS | 25.0% |
| !!! | TTTTTGTTTCCATATTCTTC+TGG | + | chr5_1:46033235-46033254 | Msa0733070:CDS | 25.0% |
| !!! | TTTTGTTTCCATATTCTTCT+GGG | + | chr5_1:46033236-46033255 | Msa0733070:CDS | 25.0% |
| ! | ATATATACACACATAATTGG+AGG | + | chr5_1:46033869-46033888 | Msa0733070:intron | 25.0% |
| TTTGTTTCCATATTCTTCTG+GGG | + | chr5_1:46033237-46033256 | Msa0733070:CDS | 30.0% | |
| !!! | TGTATTGTTTTTTGGTAAGC+AGG | + | chr5_1:46033911-46033930 | Msa0733070:intron | 30.0% |
| ATTGTGATACTTTCATCTGT+TGG | - | chr5_1:46034029-46034048 | None:intergenic | 30.0% | |
| TTTGTTTCCATATTCTTCTG+GGG | + | chr5_1:46033237-46033256 | Msa0733070:CDS | 30.0% | |
| !!! | TGTATTGTTTTTTGGTAAGC+AGG | + | chr5_1:46033911-46033930 | Msa0733070:intron | 30.0% |
| ATTGTGATACTTTCATCTGT+TGG | - | chr5_1:46034029-46034048 | None:intergenic | 30.0% | |
| GCAGCAACTAAAATAACCAT+TGG | + | chr5_1:46033124-46033143 | Msa0733070:CDS | 35.0% | |
| !! | AATAACCATTGGTCTTCCAT+TGG | + | chr5_1:46033135-46033154 | Msa0733070:CDS | 35.0% |
| !! | ATAACCATTGGTCTTCCATT+GGG | + | chr5_1:46033136-46033155 | Msa0733070:CDS | 35.0% |
| TTAATGACCCCAGAAGAATA+TGG | - | chr5_1:46033247-46033266 | None:intergenic | 35.0% | |
| ATTACCACAAAAGGAAATGC+TGG | - | chr5_1:46033316-46033335 | None:intergenic | 35.0% | |
| GGGAAGATAATTACCACAAA+AGG | - | chr5_1:46033325-46033344 | None:intergenic | 35.0% | |
| ! | ATGCCATGGCTATGAATTTT+GGG | - | chr5_1:46033992-46034011 | None:intergenic | 35.0% |
| GCAGCAACTAAAATAACCAT+TGG | + | chr5_1:46033124-46033143 | Msa0733070:CDS | 35.0% | |
| !! | AATAACCATTGGTCTTCCAT+TGG | + | chr5_1:46033135-46033154 | Msa0733070:CDS | 35.0% |
| !! | ATAACCATTGGTCTTCCATT+GGG | + | chr5_1:46033136-46033155 | Msa0733070:CDS | 35.0% |
| TTAATGACCCCAGAAGAATA+TGG | - | chr5_1:46033247-46033266 | None:intergenic | 35.0% | |
| ATTACCACAAAAGGAAATGC+TGG | - | chr5_1:46033316-46033335 | None:intergenic | 35.0% | |
| GGGAAGATAATTACCACAAA+AGG | - | chr5_1:46033325-46033344 | None:intergenic | 35.0% | |
| ! | ATGCCATGGCTATGAATTTT+GGG | - | chr5_1:46033992-46034011 | None:intergenic | 35.0% |
| TAAACCCAATGGAAGACCAA+TGG | - | chr5_1:46033143-46033162 | None:intergenic | 40.0% | |
| !! | AAGAGAAGTGCTAAACCCAA+TGG | - | chr5_1:46033154-46033173 | None:intergenic | 40.0% |
| !! | AAAGGAAATGCTGGTTTCTG+AGG | - | chr5_1:46033307-46033326 | None:intergenic | 40.0% |
| ! | GAAACCAGCATTTCCTTTTG+TGG | + | chr5_1:46033309-46033328 | Msa0733070:CDS | 40.0% |
| !! | ACTAGTTAAGGAAGGCACTA+AGG | - | chr5_1:46033542-46033561 | None:intergenic | 40.0% |
| !!! | AATTTTGGGATCTCATCTCC+TGG | - | chr5_1:46033978-46033997 | None:intergenic | 40.0% |
| GATCCCAAAATTCATAGCCA+TGG | + | chr5_1:46033986-46034005 | Msa0733070:CDS | 40.0% | |
| ! | CATGCCATGGCTATGAATTT+TGG | - | chr5_1:46033993-46034012 | None:intergenic | 40.0% |
| TCATCTGTTGGAGGTTTACA+TGG | - | chr5_1:46034017-46034036 | None:intergenic | 40.0% | |
| GTGATACTTTCATCTGTTGG+AGG | - | chr5_1:46034026-46034045 | None:intergenic | 40.0% | |
| TATCACAATCCATGTGCACA+AGG | + | chr5_1:46034040-46034059 | Msa0733070:CDS | 40.0% | |
| ! | TTGTTTCTTCCTTGTGCACA+TGG | - | chr5_1:46034052-46034071 | None:intergenic | 40.0% |
| TAAACCCAATGGAAGACCAA+TGG | - | chr5_1:46033143-46033162 | None:intergenic | 40.0% | |
| !! | AAGAGAAGTGCTAAACCCAA+TGG | - | chr5_1:46033154-46033173 | None:intergenic | 40.0% |
| !! | AAAGGAAATGCTGGTTTCTG+AGG | - | chr5_1:46033307-46033326 | None:intergenic | 40.0% |
| ! | GAAACCAGCATTTCCTTTTG+TGG | + | chr5_1:46033309-46033328 | Msa0733070:CDS | 40.0% |
| !! | ACTAGTTAAGGAAGGCACTA+AGG | - | chr5_1:46033542-46033561 | None:intergenic | 40.0% |
| !!! | AATTTTGGGATCTCATCTCC+TGG | - | chr5_1:46033978-46033997 | None:intergenic | 40.0% |
| GATCCCAAAATTCATAGCCA+TGG | + | chr5_1:46033986-46034005 | Msa0733070:CDS | 40.0% | |
| ! | CATGCCATGGCTATGAATTT+TGG | - | chr5_1:46033993-46034012 | None:intergenic | 40.0% |
| TCATCTGTTGGAGGTTTACA+TGG | - | chr5_1:46034017-46034036 | None:intergenic | 40.0% | |
| GTGATACTTTCATCTGTTGG+AGG | - | chr5_1:46034026-46034045 | None:intergenic | 40.0% | |
| TATCACAATCCATGTGCACA+AGG | + | chr5_1:46034040-46034059 | Msa0733070:CDS | 40.0% | |
| ! | TTGTTTCTTCCTTGTGCACA+TGG | - | chr5_1:46034052-46034071 | None:intergenic | 40.0% |
| !! | AAATGCTGGTTTCTGAGGAG+AGG | - | chr5_1:46033302-46033321 | None:intergenic | 45.0% |
| AGGTTTACATGGACATGCCA+TGG | - | chr5_1:46034006-46034025 | None:intergenic | 45.0% | |
| !! | AAATGCTGGTTTCTGAGGAG+AGG | - | chr5_1:46033302-46033321 | None:intergenic | 45.0% |
| AGGTTTACATGGACATGCCA+TGG | - | chr5_1:46034006-46034025 | None:intergenic | 45.0% | |
| TCATCTCCTGGCATCAACAC+AGG | - | chr5_1:46033966-46033985 | None:intergenic | 50.0% | |
| TCATCTCCTGGCATCAACAC+AGG | - | chr5_1:46033966-46033985 | None:intergenic | 50.0% | |
| ! | AGCTTGCCTGTGTTGATGCC+AGG | + | chr5_1:46033957-46033976 | Msa0733070:CDS | 55.0% |
| ! | AGCTTGCCTGTGTTGATGCC+AGG | + | chr5_1:46033957-46033976 | Msa0733070:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 46033106 | 46034088 | 46033106 | ID=Msa0733070;Name=Msa0733070 |
| chr5_1 | mRNA | 46033106 | 46034088 | 46033106 | ID=Msa0733070-mRNA-1;Parent=Msa0733070;Name=Msa0733070-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|126 |
| chr5_1 | exon | 46033106 | 46033330 | 46033106 | ID=Msa0733070-mRNA-1:exon:13131;Parent=Msa0733070-mRNA-1 |
| chr5_1 | exon | 46033933 | 46034088 | 46033933 | ID=Msa0733070-mRNA-1:exon:13132;Parent=Msa0733070-mRNA-1 |
| chr5_1 | CDS | 46033106 | 46033330 | 46033106 | ID=Msa0733070-mRNA-1:cds;Parent=Msa0733070-mRNA-1 |
| chr5_1 | CDS | 46033933 | 46034088 | 46033933 | ID=Msa0733070-mRNA-1:cds;Parent=Msa0733070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
