AlfalfaGEDB | Msa0733620

M. sativa cultivar zhongmu-4 / Msa0733620

KEGG: K15032
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009303 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0018130 GO:0019438 GO:0019843 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0032501 GO:0032502 GO:0032774 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0042254 GO:0042255 GO:0042793 GO:0042794 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:0097659 GO:0098781 GO:1901360 GO:1901362 GO:1901363 GO:1901576
PFAM: PF02536.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733620	XP_003615503.1	95.695	511	19	1	63	570	1	511	0.0	972

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733620	sp\|Q9ZT96\|MTEF4_ARATH	67.048	525	149	5	62	569	2	519	0.0	675

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733620	G7K677	95.695	511	19	1	63	570	1	511	0.0	972

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TFs/TRs:

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Gene ID	Type	Classification
Msa0733620	TR	mTERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0733620	Msa0774740	0.990905	6.608501e-185	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733620	MtrunA17_Chr5g0428941	95.695	511	19	1	63	570	1	511	0.0	972
Msa0733620	MtrunA17_Chr1g0212691	53.250	400	185	1	162	561	71	468	2.19e-153	450
Msa0733620	MtrunA17_Chr8g0391881	47.339	357	157	1	162	518	49	374	3.95e-111	337
Msa0733620	MtrunA17_Chr4g0034981	26.705	352	252	3	195	541	235	585	5.33e-35	140
Msa0733620	MtrunA17_Chr4g0043941	23.397	312	201	4	235	544	46	321	4.22e-31	124
Msa0733620	MtrunA17_Chr4g0043941	22.318	233	176	2	200	427	43	275	7.86e-17	82.0
Msa0733620	MtrunA17_Chr2g0294671	28.425	292	199	7	265	553	210	494	2.13e-29	122
Msa0733620	MtrunA17_Chr2g0294671	21.986	282	171	7	198	434	211	488	4.89e-13	71.6
Msa0733620	MtrunA17_Chr5g0445491	26.061	330	195	9	231	551	192	481	5.72e-25	108
Msa0733620	MtrunA17_Chr5g0445491	24.153	236	166	5	193	420	221	451	2.11e-12	69.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733620	AT4G02990.2	67.048	525	149	5	62	569	2	519	0.0	675
Msa0733620	AT4G02990.1	67.048	525	149	5	62	569	2	519	0.0	675
Msa0733620	AT2G44020.1	49.752	404	201	1	162	565	76	477	6.50e-144	426
Msa0733620	AT2G21710.1	26.854	391	276	7	194	579	241	626	3.81e-39	152
Msa0733620	AT2G21710.1	24.460	278	194	6	161	423	274	550	2.90e-17	85.9
Msa0733620	AT4G38160.4	24.638	276	205	3	278	550	54	329	4.05e-32	127
Msa0733620	AT4G38160.4	20.705	227	167	5	196	410	39	264	3.13e-14	74.7
Msa0733620	AT4G38160.1	24.638	276	205	3	278	550	54	329	4.05e-32	127
Msa0733620	AT4G38160.1	20.705	227	167	5	196	410	39	264	3.13e-14	74.7
Msa0733620	AT4G38160.2	24.638	276	205	3	278	550	54	329	5.77e-32	127

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Find 149 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCATAAGTGAAGAATGC+TGG	0.479920	5_1:+46938159	None:intergenic
AAATCAACGTGCTTCAGCAT+AGG	0.578326	5_1:+46938333	None:intergenic
AAATCGAATCCAAGAATATA+AGG	0.400009	5_1:+46938579	None:intergenic
AAATTCAAAGGAAACGGGGA+GGG	0.552308	5_1:-46939244	Msa0733620:CDS
AACAACTATCCTCTTGTACT+GGG	0.560648	5_1:-46939020	Msa0733620:CDS
AACAATAGGATGAAAATATT+TGG	0.206968	5_1:+46937767	None:intergenic
AACCATAAGTGAAGAATGCT+GGG	0.531139	5_1:+46938160	None:intergenic
AACTAGATTCCCGGAGATTT+TGG	0.122651	5_1:-46938697	Msa0733620:CDS
AACTCAACCAAATCTACCAA+AGG	0.619874	5_1:+46938183	None:intergenic
AAGATTCAATGTTCTGTTGC+TGG	0.373247	5_1:-46939329	Msa0733620:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGTGTTTGTGTTTGTGTT+GGG	+	chr5_1:46937678-46937697	None:intergenic	30.0%
TGATTGATAGGAAGAAACAT+AGG	-	chr5_1:46937976-46937995	Msa0733620:CDS	30.0%
GATTGATAGGAAGAAACATA+GGG	-	chr5_1:46937977-46937996	Msa0733620:CDS	30.0%
AGATTACATGAATGAGTACA+TGG	-	chr5_1:46938036-46938055	Msa0733620:CDS	30.0%
TTAGTTAAGGTAGTTAGAGT+TGG	+	chr5_1:46938134-46938153	None:intergenic	30.0%
TTAGAATTGGCATTTCTGTT+GGG	+	chr5_1:46938336-46938355	None:intergenic	30.0%
GAAATGCCAATTCTAACATT+TGG	-	chr5_1:46938340-46938359	Msa0733620:CDS	30.0%
GAATGACCAAATGTTAGAAT+TGG	+	chr5_1:46938349-46938368	None:intergenic	30.0%
TAATTTCAGATAAACTCGGA+CGG	+	chr5_1:46938567-46938586	None:intergenic	30.0%
ATGTAGTGTCAAGAAGAATA+TGG	-	chr5_1:46938771-46938790	Msa0733620:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	46937646	46940144	46937646	ID=Msa0733620;Name=Msa0733620
chr5_1	mRNA	46937646	46940144	46937646	ID=Msa0733620-mRNA-1;Parent=Msa0733620;Name=Msa0733620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=14\|0\|0.66\|0.66\|0\|0.33\|3\|257\|594
chr5_1	exon	46937646	46939528	46937646	ID=Msa0733620-mRNA-1:exon:13435;Parent=Msa0733620-mRNA-1
chr5_1	exon	46939733	46939752	46939733	ID=Msa0733620-mRNA-1:exon:13434;Parent=Msa0733620-mRNA-1
chr5_1	exon	46939992	46940144	46939992	ID=Msa0733620-mRNA-1:exon:13433;Parent=Msa0733620-mRNA-1
chr5_1	five_prime_UTR	46940131	46940144	46940131	ID=Msa0733620-mRNA-1:five_prime_utr;Parent=Msa0733620-mRNA-1
chr5_1	CDS	46939992	46940130	46939992	ID=Msa0733620-mRNA-1:cds;Parent=Msa0733620-mRNA-1
chr5_1	CDS	46939733	46939752	46939733	ID=Msa0733620-mRNA-1:cds;Parent=Msa0733620-mRNA-1
chr5_1	CDS	46937903	46939528	46937903	ID=Msa0733620-mRNA-1:cds;Parent=Msa0733620-mRNA-1
chr5_1	three_prime_UTR	46937646	46937902	46937646	ID=Msa0733620-mRNA-1:three_prime_utr;Parent=Msa0733620-mRNA-1

Gene Sequence

>Msa0733620

ATGAAATCGATCTTTCCCAACACAAACACAAACACAAACAGGGCAGAAAAACCCTCTTCCAGTGTCTGTTATCCCTTATCAGTGTTCAAGCAAGAACCAAAGCAGTCGAACTTCAATTCAACAACACACTCTTGTCACTATTACATGAATGAGTACATGATTCAATGGTTTCCAATTCTTAAAAATATGAACTTTTCTGGTACCATAACAAAACCCAATTTCCCATTTCCCCCAACAGAAATGCCAATTCTAACATTTGGTCATTCTAAGTTAACTGCTGCTACACTAGCTCTTCAATTGCCATGTAATTGTATTAGGAAGATAAGGTTTATTACAAAGATTCAATGTTCTGTTGCTGGTAGGACGTATAGTTATAGGACCGGTGCTGCTGATTCGCGGGGATCCAAACGGGACTCTGGCGAAATTCAAAGGAAACGGGGAGGGGCATCGTCGTTGTATGCCCGTCCGAGTTTATCTGAAATTAAGAAAGATAAGGCCACGTTGCGTGAAATTGTTTATGAGTTCTTGCGAGGAATCGGCATTGTTCCTGATGAACTTGATGGTCTTGAACTTCCTGTTACGGTTGATGTTATGAAGGAGCGTGTTGATTTTCTTCACAGTTTGGGGCTTACGATTGAAGACATTAACAACTATCCTCTTGTACTGGGATGTAGTGTCAAGAAGAATATGGTTCCTGTGCTTGATTACCTTGGTAAATTGGGAGTTAGGAAATCCACGTTAACGCAGTTCTTGCGGAGATATCCACAAGTGCTTCATTCTAGTGTGGTTGTGGATCTTGTGCCGGTGGTCAAGTATCTTCAGGGGATGGATATCAAACCGGATGATATTCCTCGTGTTCTTGAGAGGTATCCTGAAGTGTTGGGATTCAAACTTGAGGGAACCATGAGTACATCTGTTGCTTATTTGATTGGAATCGGTGTTGGAAGAAGAGAACTCGGAGGCATCTTAACTAGATTCCCGGAGATTTTGGGGATGCGGGTTGGTAGAGTCATCAAGCCTTTTGTGGAGTATCTGGAAAGCTTGGGTATTCCAAGGCTAGCTATTGCTAGACTGATAGAGACACAACCTTATATTCTTGGATTCGATTTGGACGAGAAAGTGAAGTCAAATGTCAAATCCCTCGAAGAGTTTAATGTTCGGCAGACATTGCTTGCCTCTATAATTGCTCAGTATCCTGACATCATTGGAACCGACCTAGAGCCGAAGCTTGCAGATAAGAGAAGTGTGCTCAATTCTGTGCTTGATTTGGATGTTGAGGATTTCGGCTTAATCATTGAGAAGATGCCGCAGGTAGTTAGCCTCAGTACTACACCTATGCTGAAGCACGTTGATTTTCTTAAGGATTGTGGGTTTTCTGTGGATCAAATGAGGAAGATGATTGTTGGATGCCCTCAACTACTTGCTTTAAACATTGATATCATGAAACTTAGCTTCGATTACTTCCAAAGTGAGATGGAAAGGCCTTTGGTAGATTTGGTTGAGTTCCCAGCATTCTTCACTTATGGTTTGGAGTCAACTATAAAACCTAGGCATAACATGGTTGCTAAGAAAGGATTGAAGTGTTCTCTGGCATGGATGCTTAATTGTTCTGATGAGAAATTTGAGCAACGAATGGACTATGACACTATTGATATGGAAGAGATGGAAATGGAACCATCATTTGACATGAATTCATTGATGCGGCCAAGGAGCGATGAATCAGATTCTGATTATGAAGACAGTGATTATGATGATGATGATGATGATGATGATGATGATGAGTAA

Protein sequence

>Msa0733620

MKSIFPNTNTNTNRAEKPSSSVCYPLSVFKQEPKQSNFNSTTHSCHYYMNEYMIQWFPILKNMNFSGTITKPNFPFPPTEMPILTFGHSKLTAATLALQLPCNCIRKIRFITKIQCSVAGRTYSYRTGAADSRGSKRDSGEIQRKRGGASSLYARPSLSEIKKDKATLREIVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTLTQFLRRYPQVLHSSVVVDLVPVVKYLQGMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLGIPRLAIARLIETQPYILGFDLDEKVKSNVKSLEEFNVRQTLLASIIAQYPDIIGTDLEPKLADKRSVLNSVLDLDVEDFGLIIEKMPQVVSLSTTPMLKHVDFLKDCGFSVDQMRKMIVGCPQLLALNIDIMKLSFDYFQSEMERPLVDLVEFPAFFTYGLESTIKPRHNMVAKKGLKCSLAWMLNCSDEKFEQRMDYDTIDMEEMEMEPSFDMNSLMRPRSDESDSDYEDSDYDDDDDDDDDDE*