AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0734170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0734170 MtrunA17_Chr5g0429771 59.146 328 111 6 1 325 1 308 5.16e-133 380
Msa0734170 MtrunA17_Chr5g0429811 93.836 146 9 0 183 328 23 168 7.97e-98 286
Msa0734170 MtrunA17_Chr5g0429781 76.978 139 32 0 187 325 1 139 1.72e-77 233
Msa0734170 MtrunA17_Chr5g0429821 81.452 124 15 1 1 124 1 116 4.10e-65 201
Msa0734170 MtrunA17_Chr5g0429791 54.217 166 56 8 1 163 1 149 1.54e-45 153
Msa0734170 MtrunA17_Chr8g0370491 28.378 148 102 3 94 238 33 179 4.23e-12 66.2
Msa0734170 MtrunA17_Chr1g0199941 27.703 148 103 3 94 238 33 179 5.57e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0734170 AT5G58770.1 60.684 234 91 1 92 325 75 307 1.05e-108 319
Msa0734170 AT5G58782.1 51.464 239 115 1 86 324 49 286 1.26e-86 262
Msa0734170 AT5G58782.2 51.464 239 115 1 86 324 67 304 1.67e-86 263
Msa0734170 AT5G58780.1 49.242 264 128 3 62 324 41 299 1.36e-83 255
Msa0734170 AT2G23410.1 49.367 237 118 2 89 324 65 300 1.46e-78 242
Msa0734170 AT5G58784.1 50.893 224 109 1 93 316 69 291 7.18e-76 235
Msa0734170 AT2G23400.1 45.643 241 107 3 88 324 64 284 5.05e-66 209
Msa0734170 AT2G17570.1 32.114 246 145 6 94 318 39 283 1.69e-29 114
Msa0734170 AT2G23400.2 43.902 123 68 1 88 210 64 185 9.98e-28 107
Msa0734170 AT5G60510.1 27.119 236 155 6 94 320 35 262 1.39e-20 90.1
Msa0734170 AT5G60500.1 26.695 236 156 6 94 320 34 261 1.93e-19 87.0

Find 105 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTAACTTGTCGAATTTA+AGG 0.159834 5_1:+47826059 Msa0734170:three_prime_UTR
GAACCAAAACACAACATTTA+TGG 0.192674 5_1:-47826395 None:intergenic
ATGCAATGTCATAGATTATT+TGG 0.198275 5_1:+47826115 Msa0734170:three_prime_UTR
GACATTGATGAAAACATTAT+TGG 0.199481 5_1:+47825773 Msa0734170:CDS
TCGTGTTGTCTACTGAAAAC+TGG 0.261670 5_1:+47824355 Msa0734170:CDS
AAACATTATTGGAAATGATT+TGG 0.276316 5_1:+47825784 Msa0734170:CDS
ATTGTTGTTGTGACAATATT+GGG 0.277614 5_1:+47826363 Msa0734170:three_prime_UTR
TATAAATAGTGATGAGATTA+AGG 0.287830 5_1:+47826173 Msa0734170:three_prime_UTR
AAGGAGTCCTCAAGGGGTTT+TGG 0.290367 5_1:+47826082 Msa0734170:three_prime_UTR
GCAGAGTTGGTTGTTGTCTT+TGG 0.291915 5_1:-47823979 None:intergenic
TCTACATCTGTCTAGTAATA+TGG 0.294458 5_1:+47826020 Msa0734170:three_prime_UTR
CAACTTCTTGTTATGGCAAT+TGG 0.302761 5_1:+47825874 Msa0734170:CDS
CTTATTGTGGCAATTAACTA+TGG 0.304487 5_1:+47825677 Msa0734170:CDS
TATTGTTGTTGTGACAATAT+TGG 0.343853 5_1:+47826362 Msa0734170:three_prime_UTR
TGGCCATAAATGTTGTGTTT+TGG 0.369703 5_1:+47826392 Msa0734170:three_prime_UTR
TCTCTGTTGAAATGAACTTA+AGG 0.383234 5_1:-47825963 None:intergenic
GAGTTAGCAACTTCTTGTTA+TGG 0.399212 5_1:+47825867 Msa0734170:CDS
ACAATTTCACCACCCTTCTC+AGG 0.401610 5_1:-47824299 None:intergenic
CAACAGAGAGAAAGACGTTT+CGG 0.404751 5_1:+47825977 Msa0734170:CDS
TGAGGTCATATAGCTTATGT+TGG 0.419548 5_1:+47826420 Msa0734170:three_prime_UTR
GAGAGGGTTGCCGCCGTTAG+AGG 0.425623 5_1:+47824254 Msa0734170:CDS
GAGGTCATATAGCTTATGTT+GGG 0.429695 5_1:+47826421 Msa0734170:three_prime_UTR
TCATACTCAAGTGTCGTGTT+TGG 0.431097 5_1:-47824108 None:intergenic
CTCGAAGTTCTCCACCGGTC+CGG 0.432624 5_1:-47825847 None:intergenic
CCGGTCCGGATTAGTAGATC+AGG 0.433526 5_1:-47825833 None:intergenic
TGGTGAAATTGTGTTTAAGC+TGG 0.439634 5_1:+47824310 Msa0734170:CDS
ATGAGAAGAAGGAGCAGAGT+TGG 0.440153 5_1:-47823992 None:intergenic
TCAGCAGCAAGTCTTCATCT+AGG 0.445448 5_1:-47824152 None:intergenic
GTAATTTCTTGTATCTTGCT+CGG 0.447525 5_1:+47826500 Msa0734170:three_prime_UTR
AGAATTAAAATATCTGTGAT+TGG 0.450076 5_1:+47825572 Msa0734170:CDS
TTGGTTGTTGTCTTTGGTGG+TGG 0.452574 5_1:-47823973 None:intergenic
CCTTCTCAGGGGCTGCACTA+AGG 0.457323 5_1:-47824286 None:intergenic
CGTGTTGTCTACTGAAAACT+GGG 0.460003 5_1:+47824356 Msa0734170:CDS
ACAATCACCGCCACATGCTT+TGG 0.466252 5_1:-47824204 None:intergenic
TGAAGACTTGCTGCTGATGC+CGG 0.470492 5_1:+47824158 Msa0734170:CDS
TCAAGTGTCGTGTTTGGAAT+CGG 0.475730 5_1:-47824102 None:intergenic
GAGTTGGTTGTTGTCTTTGG+TGG 0.482522 5_1:-47823976 None:intergenic
AGCTCCTCCTTCAGCTCTGC+CGG 0.487482 5_1:-47824177 None:intergenic
GCTGATGCCGGCAGAGCTGA+AGG 0.491822 5_1:+47824170 Msa0734170:CDS
CTTACCCTGATCTACTAATC+CGG 0.493702 5_1:+47825828 Msa0734170:CDS
AGAGAAAGACGTTTCGGTGG+TGG 0.495173 5_1:+47825983 Msa0734170:CDS
GTGAAGGGAATCGTTGTGAT+TGG 0.495903 5_1:-47824032 None:intergenic
CCTTAGTGCAGCCCCTGAGA+AGG 0.496357 5_1:+47824286 Msa0734170:CDS
CGGATTAGTAGATCAGGGTA+AGG 0.498287 5_1:-47825827 None:intergenic
GAGAAAGACGTTTCGGTGGT+GGG 0.501667 5_1:+47825984 Msa0734170:CDS
GAATCGTTGTGATTGGTAAC+GGG 0.503028 5_1:-47824025 None:intergenic
AAACACGACACTTGAGTATG+AGG 0.505589 5_1:+47824110 Msa0734170:CDS
CAACAGACATTGCAACTAGT+AGG 0.509971 5_1:-47826478 None:intergenic
GGTGAAATTGTGTTTAAGCT+GGG 0.510118 5_1:+47824311 Msa0734170:CDS
TTGTGGATGGACACAGGAGG+TGG 0.512018 5_1:+47824223 Msa0734170:CDS
AGTTAATTGCCACAATAAGA+TGG 0.517463 5_1:-47825673 None:intergenic
CGTAGTGCAACTGACACGTT+TGG 0.521119 5_1:-47824070 None:intergenic
GCTTATGTTGGGTTCTTGAG+TGG 0.523148 5_1:+47826432 Msa0734170:three_prime_UTR
GACACGTTTGGTAGCAACCA+TGG 0.529990 5_1:-47824058 None:intergenic
CAATTTCACCACCCTTCTCA+GGG 0.534290 5_1:-47824298 None:intergenic
AATTGATTGTTCTTTCAAAG+AGG 0.539018 5_1:-47825343 None:intergenic
TAACTTGTCGAATTTAAGGA+AGG 0.545394 5_1:+47826063 Msa0734170:three_prime_UTR
GTAGCAACCATGGGTTGTGA+AGG 0.545893 5_1:-47824048 None:intergenic
ACGATTCCCTTCACAACCCA+TGG 0.548004 5_1:+47824041 Msa0734170:CDS
AAAGCATGTGGCGGTGATTG+TGG 0.550782 5_1:+47824206 Msa0734170:CDS
GAATGATGGTAATGAGAAGA+AGG 0.550838 5_1:-47824003 None:intergenic
GGAATCGTTGTGATTGGTAA+CGG 0.551334 5_1:-47824026 None:intergenic
CTTAGTGCAGCCCCTGAGAA+GGG 0.554713 5_1:+47824287 Msa0734170:CDS
ACACGTTTGGTAGCAACCAT+GGG 0.556516 5_1:-47824057 None:intergenic
TTGTTGTTGTGACAATATTG+GGG 0.556562 5_1:+47826364 Msa0734170:three_prime_UTR
CAGCAGCAAGTCTTCATCTA+GGG 0.560968 5_1:-47824151 None:intergenic
TTCAAGACTCCATCTTATTG+TGG 0.564037 5_1:+47825664 Msa0734170:CDS
GAAAATGATAGCTTATATTG+AGG 0.566357 5_1:+47825625 Msa0734170:CDS
AGAGGGTTGCCGCCGTTAGA+GGG 0.567476 5_1:+47824255 Msa0734170:CDS
GTGATTGGTAACGGGAATGA+TGG 0.580395 5_1:-47824017 None:intergenic
TTTAAGGAAGGAGTCCTCAA+GGG 0.588541 5_1:+47826075 Msa0734170:three_prime_UTR
GGAGCTGATGCCAAAGCATG+TGG 0.592367 5_1:+47824194 Msa0734170:CDS
CGGTGATTGTGGATGGACAC+AGG 0.593106 5_1:+47824217 Msa0734170:CDS
ATTTAAGGAAGGAGTCCTCA+AGG 0.602761 5_1:+47826074 Msa0734170:three_prime_UTR
CATGTGGCGGTGATTGTGGA+TGG 0.603473 5_1:+47824210 Msa0734170:CDS
AAACTGGGTTCTTCGTCCGA+AGG 0.603586 5_1:+47824371 Msa0734170:CDS
CAAACGTGTCAGTTGCACTA+CGG 0.603739 5_1:+47824071 Msa0734170:CDS
ATTGTGGCAATTAACTATGG+AGG 0.616175 5_1:+47825680 Msa0734170:CDS
TAGCAACCATGGGTTGTGAA+GGG 0.621250 5_1:-47824047 None:intergenic
TTAAGGAAGGAGTCCTCAAG+GGG 0.623460 5_1:+47826076 Msa0734170:three_prime_UTR
AGGAGGTGGGCGAAAACGAG+AGG 0.627083 5_1:+47824237 Msa0734170:CDS
GTGAAATTGAAGCAATCATG+AGG 0.627184 5_1:+47825366 Msa0734170:CDS
AAGGCTACATATCCCTCTAA+CGG 0.630716 5_1:-47824267 None:intergenic
GCTAACTCGAAGTTCTCCAC+CGG 0.631182 5_1:-47825852 None:intergenic
GAATCAACCAAAACCCCTTG+AGG 0.633142 5_1:-47826089 None:intergenic
CCTGATCTACTAATCCGGAC+CGG 0.638393 5_1:+47825833 Msa0734170:CDS
GATGCCGGCAGAGCTGAAGG+AGG 0.639350 5_1:+47824173 Msa0734170:CDS
GATCTACTAATCCGGACCGG+TGG 0.640608 5_1:+47825836 Msa0734170:CDS
TGTGGATGGACACAGGAGGT+GGG 0.642197 5_1:+47824224 Msa0734170:CDS
GTGAAATTGTGTTTAAGCTG+GGG 0.649532 5_1:+47824312 Msa0734170:CDS
CGGTCCGGATTAGTAGATCA+GGG 0.652120 5_1:-47825832 None:intergenic
GCTACATATCCCTCTAACGG+CGG 0.653396 5_1:-47824264 None:intergenic
GGATTAGTAGATCAGGGTAA+GGG 0.654177 5_1:-47825826 None:intergenic
ACACTTGAGTATGAGGCGGA+AGG 0.657748 5_1:+47824117 Msa0734170:CDS
AGTGCAGCCCCTGAGAAGGG+TGG 0.660366 5_1:+47824290 Msa0734170:CDS
GGAGGTGGGCGAAAACGAGA+GGG 0.662802 5_1:+47824238 Msa0734170:CDS
GTAGCTAAGAAAGTGCAAGA+TGG 0.665247 5_1:+47825734 Msa0734170:CDS
CACGACACTTGAGTATGAGG+CGG 0.670474 5_1:+47824113 Msa0734170:CDS
CAGAGAGAAAGACGTTTCGG+TGG 0.672578 5_1:+47825980 Msa0734170:CDS
GATGAGATTAAGGTGCAATG+TGG 0.680683 5_1:+47826183 Msa0734170:three_prime_UTR
TGGCGCATCAAAATACAACA+AGG 0.688452 5_1:-47826313 None:intergenic
TTATATTGAGGAGAAGACAA+AGG 0.692258 5_1:+47825637 Msa0734170:CDS
AATTTCACCACCCTTCTCAG+GGG 0.693658 5_1:-47824297 None:intergenic
GCTGATGCCAAAGCATGTGG+CGG 0.720416 5_1:+47824197 Msa0734170:CDS
TGATTGTGGATGGACACAGG+AGG 0.793152 5_1:+47824220 Msa0734170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTATTTTTTATATT+TGG + chr5_1:47824874-47824893 Msa0734170:intron 0.0%
!!! TTTAAAACAATAAAATTGTT+TGG - chr5_1:47824639-47824658 None:intergenic 10.0%
!!! AAACAATTTTATTGTTTTAA+AGG + chr5_1:47824638-47824657 Msa0734170:intron 10.0%
!!! AACAATTTTATTGTTTTAAA+GGG + chr5_1:47824639-47824658 Msa0734170:intron 10.0%
!! CATATTATCTTATATATATT+TGG - chr5_1:47824973-47824992 None:intergenic 10.0%
!! AAGAAGAAAACTTAAATAAT+TGG + chr5_1:47824567-47824586 Msa0734170:intron 15.0%
!! TAGCCAAATAATATATTAAT+GGG - chr5_1:47824614-47824633 None:intergenic 15.0%
!!! TACAGTTTACTTTATTTTAA+TGG + chr5_1:47825246-47825265 Msa0734170:intron 15.0%
!!! ACAGTTTACTTTATTTTAAT+GGG + chr5_1:47825247-47825266 Msa0734170:intron 15.0%
!!! AGTTATTAGAGTGTTTTTTA+GGG - chr5_1:47823950-47823969 None:intergenic 20.0%
!!! AAGTTATTAGAGTGTTTTTT+AGG - chr5_1:47823951-47823970 None:intergenic 20.0%
!! AAAATTGAAATTATCACCTT+CGG - chr5_1:47824390-47824409 None:intergenic 20.0%
!!! TTAAGTTTTCTTCTTCTTTT+GGG - chr5_1:47824562-47824581 None:intergenic 20.0%
!!! TTTAAGTTTTCTTCTTCTTT+TGG - chr5_1:47824563-47824582 None:intergenic 20.0%
!! TACCCCATTAATATATTATT+TGG + chr5_1:47824608-47824627 Msa0734170:intron 20.0%
!! AGCCAAATAATATATTAATG+GGG - chr5_1:47824613-47824632 None:intergenic 20.0%
!! GTAGCCAAATAATATATTAA+TGG - chr5_1:47824615-47824634 None:intergenic 20.0%
!! CGACAACTTATATTATATAT+AGG - chr5_1:47825029-47825048 None:intergenic 20.0%
!!! TTTTATAGATCCACATAATT+TGG + chr5_1:47825106-47825125 Msa0734170:intron 20.0%
!! TTCTTTCTCTATTATAAGAA+AGG - chr5_1:47825556-47825575 None:intergenic 20.0%
!! AGAATTAAAATATCTGTGAT+TGG + chr5_1:47825572-47825591 Msa0734170:CDS 20.0%
!! AAACATTATTGGAAATGATT+TGG + chr5_1:47825784-47825803 Msa0734170:CDS 20.0%
!!! TTTTCTTTAATCGAAAACTT+TGG + chr5_1:47825909-47825928 Msa0734170:CDS 20.0%
!!! AAATCATTTTTCTCAAAATC+TGG - chr5_1:47825935-47825954 None:intergenic 20.0%
!! ATTATTTGGAAAGCTAAAAA+TGG + chr5_1:47826129-47826148 Msa0734170:three_prime_UTR 20.0%
!! TATAAATAGTGATGAGATTA+AGG + chr5_1:47826173-47826192 Msa0734170:three_prime_UTR 20.0%
!! AAAACGAAAAATATTCTTGA+AGG - chr5_1:47826550-47826569 None:intergenic 20.0%
! ATTATTTGCAAATGAGACAA+TGG - chr5_1:47824427-47824446 None:intergenic 25.0%
!! TAAGTTTTCTTCTTCTTTTG+GGG - chr5_1:47824561-47824580 None:intergenic 25.0%
!! GTTTAGTTTAGTGTCAATTA+TGG + chr5_1:47824691-47824710 Msa0734170:intron 25.0%
! GTTATTCGAACAATTATTCT+TGG + chr5_1:47824931-47824950 Msa0734170:intron 25.0%
! AATTATTCTTGGATAACCTA+TGG + chr5_1:47824942-47824961 Msa0734170:intron 25.0%
! TATATATTTGGTTGTTCCAT+AGG - chr5_1:47824961-47824980 None:intergenic 25.0%
! AACGGTTTAAATTCATACAT+AGG - chr5_1:47825137-47825156 None:intergenic 25.0%
! AATTGATTGTTCTTTCAAAG+AGG - chr5_1:47825346-47825365 None:intergenic 25.0%
! GAAAATGATAGCTTATATTG+AGG + chr5_1:47825625-47825644 Msa0734170:CDS 25.0%
! GACATTGATGAAAACATTAT+TGG + chr5_1:47825773-47825792 Msa0734170:CDS 25.0%
!! TGAGAAAAATGATTTTGTTG+AGG + chr5_1:47825940-47825959 Msa0734170:CDS 25.0%
!!! ATATGGTTGTTTTCTGATTT+TGG + chr5_1:47826037-47826056 Msa0734170:three_prime_UTR 25.0%
! ATGCAATGTCATAGATTATT+TGG + chr5_1:47826115-47826134 Msa0734170:three_prime_UTR 25.0%
!! TGGTATAATTTTTGCAAACA+AGG + chr5_1:47826149-47826168 Msa0734170:three_prime_UTR 25.0%
!! TATTGTTGTTGTGACAATAT+TGG + chr5_1:47826362-47826381 Msa0734170:three_prime_UTR 25.0%
!! ATTGTTGTTGTGACAATATT+GGG + chr5_1:47826363-47826382 Msa0734170:three_prime_UTR 25.0%
TCTCATTTGCAAATAATCAG+TGG + chr5_1:47824430-47824449 Msa0734170:intron 30.0%
AACCAAAAAATGTTATTCGC+AGG + chr5_1:47824498-47824517 Msa0734170:intron 30.0%
ACCAAAAAATGTTATTCGCA+GGG + chr5_1:47824499-47824518 Msa0734170:intron 30.0%
ATAATTGTTCGAATAACCAG+CGG - chr5_1:47824928-47824947 None:intergenic 30.0%
TATAGATCCACATAATTTGG+TGG + chr5_1:47825109-47825128 Msa0734170:intron 30.0%
! ATCATTTTCTGTAGAGACTT+GGG - chr5_1:47825614-47825633 None:intergenic 30.0%
! TATCATTTTCTGTAGAGACT+TGG - chr5_1:47825615-47825634 None:intergenic 30.0%
TTATATTGAGGAGAAGACAA+AGG + chr5_1:47825637-47825656 Msa0734170:CDS 30.0%
AGTTAATTGCCACAATAAGA+TGG - chr5_1:47825676-47825695 None:intergenic 30.0%
CTTATTGTGGCAATTAACTA+TGG + chr5_1:47825677-47825696 Msa0734170:CDS 30.0%
TCTCTGTTGAAATGAACTTA+AGG - chr5_1:47825966-47825985 None:intergenic 30.0%
TCTACATCTGTCTAGTAATA+TGG + chr5_1:47826020-47826039 Msa0734170:three_prime_UTR 30.0%
!! GTCTTAACTTGTCGAATTTA+AGG + chr5_1:47826059-47826078 Msa0734170:three_prime_UTR 30.0%
! TAACTTGTCGAATTTAAGGA+AGG + chr5_1:47826063-47826082 Msa0734170:three_prime_UTR 30.0%
!! TTGCTTTTAGTTAGAGTTGT+TGG + chr5_1:47826240-47826259 Msa0734170:three_prime_UTR 30.0%
!! TTTTAGTTAGAGTTGTTGGT+GGG + chr5_1:47826244-47826263 Msa0734170:three_prime_UTR 30.0%
!! ATTTTGATGCGCCAATTTTT+TGG + chr5_1:47826322-47826341 Msa0734170:three_prime_UTR 30.0%
! TTGTTGTTGTGACAATATTG+GGG + chr5_1:47826364-47826383 Msa0734170:three_prime_UTR 30.0%
GAACCAAAACACAACATTTA+TGG - chr5_1:47826398-47826417 None:intergenic 30.0%
GTAATTTCTTGTATCTTGCT+CGG + chr5_1:47826500-47826519 Msa0734170:three_prime_UTR 30.0%
!!! AGAGTGTTTTTTAGGGCAAT+GGG - chr5_1:47823943-47823962 None:intergenic 35.0%
!!! TAGAGTGTTTTTTAGGGCAA+TGG - chr5_1:47823944-47823963 None:intergenic 35.0%
! GAATGATGGTAATGAGAAGA+AGG - chr5_1:47824006-47824025 None:intergenic 35.0%
TGGTGAAATTGTGTTTAAGC+TGG + chr5_1:47824310-47824329 Msa0734170:CDS 35.0%
GGTGAAATTGTGTTTAAGCT+GGG + chr5_1:47824311-47824330 Msa0734170:CDS 35.0%
GTGAAATTGTGTTTAAGCTG+GGG + chr5_1:47824312-47824331 Msa0734170:CDS 35.0%
CATTTGCAAATAATCAGTGG+CGG + chr5_1:47824433-47824452 Msa0734170:intron 35.0%
CCAAAAAATGTTATTCGCAG+GGG + chr5_1:47824500-47824519 Msa0734170:intron 35.0%
GTGAAATTGAAGCAATCATG+AGG + chr5_1:47825366-47825385 Msa0734170:CDS 35.0%
! TCATTTTCTGTAGAGACTTG+GGG - chr5_1:47825613-47825632 None:intergenic 35.0%
TTCAAGACTCCATCTTATTG+TGG + chr5_1:47825664-47825683 Msa0734170:CDS 35.0%
ATTGTGGCAATTAACTATGG+AGG + chr5_1:47825680-47825699 Msa0734170:CDS 35.0%
GAGTTAGCAACTTCTTGTTA+TGG + chr5_1:47825867-47825886 Msa0734170:CDS 35.0%
! CAACTTCTTGTTATGGCAAT+TGG + chr5_1:47825874-47825893 Msa0734170:CDS 35.0%
!! CTTTTAGTTAGAGTTGTTGG+TGG + chr5_1:47826243-47826262 Msa0734170:three_prime_UTR 35.0%
!! ATTTGCTTTTGCAGAACACT+TGG + chr5_1:47826272-47826291 Msa0734170:three_prime_UTR 35.0%
!!! ATGCGCCAATTTTTTGGTTT+TGG + chr5_1:47826328-47826347 Msa0734170:three_prime_UTR 35.0%
!!! TGCGCCAATTTTTTGGTTTT+GGG + chr5_1:47826329-47826348 Msa0734170:three_prime_UTR 35.0%
! GTGACAATATTGGGGTTTTA+TGG + chr5_1:47826372-47826391 Msa0734170:three_prime_UTR 35.0%
! TGGCCATAAATGTTGTGTTT+TGG + chr5_1:47826392-47826411 Msa0734170:three_prime_UTR 35.0%
!!! TGTTGTGTTTTGGTTCTTTG+AGG + chr5_1:47826402-47826421 Msa0734170:three_prime_UTR 35.0%
TGAGGTCATATAGCTTATGT+TGG + chr5_1:47826420-47826439 Msa0734170:three_prime_UTR 35.0%
GAGGTCATATAGCTTATGTT+GGG + chr5_1:47826421-47826440 Msa0734170:three_prime_UTR 35.0%
!!! GAGTGTTTTTTAGGGCAATG+GGG - chr5_1:47823942-47823961 None:intergenic 40.0%
! GAATCGTTGTGATTGGTAAC+GGG - chr5_1:47824028-47824047 None:intergenic 40.0%
GGAATCGTTGTGATTGGTAA+CGG - chr5_1:47824029-47824048 None:intergenic 40.0%
! TCAAGTGTCGTGTTTGGAAT+CGG - chr5_1:47824105-47824124 None:intergenic 40.0%
! TCATACTCAAGTGTCGTGTT+TGG - chr5_1:47824111-47824130 None:intergenic 40.0%
AAACACGACACTTGAGTATG+AGG + chr5_1:47824110-47824129 Msa0734170:CDS 40.0%
AAGGCTACATATCCCTCTAA+CGG - chr5_1:47824270-47824289 None:intergenic 40.0%
TCGTGTTGTCTACTGAAAAC+TGG + chr5_1:47824355-47824374 Msa0734170:CDS 40.0%
CGTGTTGTCTACTGAAAACT+GGG + chr5_1:47824356-47824375 Msa0734170:CDS 40.0%
!!! CCCCTGCGAATAACATTTTT+TGG - chr5_1:47824503-47824522 None:intergenic 40.0%
ATAGGCTCCACCAAATTATG+TGG - chr5_1:47825119-47825138 None:intergenic 40.0%
TCCAACGCACATTCTTTGAA+CGG - chr5_1:47825155-47825174 None:intergenic 40.0%
!!! GGTTGCTGAGTTTTGATTGT+TGG + chr5_1:47825292-47825311 Msa0734170:intron 40.0%
!!! GTTTTGATTGTTGGTGCAGT+TGG + chr5_1:47825301-47825320 Msa0734170:intron 40.0%
ACAGAGTAGCAGTAAGAGTT+TGG - chr5_1:47825394-47825413 None:intergenic 40.0%
! GTAGCTAAGAAAGTGCAAGA+TGG + chr5_1:47825734-47825753 Msa0734170:CDS 40.0%
! GGATTAGTAGATCAGGGTAA+GGG - chr5_1:47825829-47825848 None:intergenic 40.0%
CTTACCCTGATCTACTAATC+CGG + chr5_1:47825828-47825847 Msa0734170:CDS 40.0%
CAACAGAGAGAAAGACGTTT+CGG + chr5_1:47825977-47825996 Msa0734170:CDS 40.0%
ATTTAAGGAAGGAGTCCTCA+AGG + chr5_1:47826074-47826093 Msa0734170:three_prime_UTR 40.0%
TTTAAGGAAGGAGTCCTCAA+GGG + chr5_1:47826075-47826094 Msa0734170:three_prime_UTR 40.0%
!!! TCAAGGGGTTTTGGTTGATT+CGG + chr5_1:47826091-47826110 Msa0734170:three_prime_UTR 40.0%
GATGAGATTAAGGTGCAATG+TGG + chr5_1:47826183-47826202 Msa0734170:three_prime_UTR 40.0%
TGGCGCATCAAAATACAACA+AGG - chr5_1:47826316-47826335 None:intergenic 40.0%
!!! GCGCCAATTTTTTGGTTTTG+GGG + chr5_1:47826330-47826349 Msa0734170:three_prime_UTR 40.0%
!!! CGCCAATTTTTTGGTTTTGG+GGG + chr5_1:47826331-47826350 Msa0734170:three_prime_UTR 40.0%
CTCCCCCAAAACCAAAAAAT+TGG - chr5_1:47826336-47826355 None:intergenic 40.0%
CAACAGACATTGCAACTAGT+AGG - chr5_1:47826481-47826500 None:intergenic 40.0%
TTGGTTGTTGTCTTTGGTGG+TGG - chr5_1:47823976-47823995 None:intergenic 45.0%
! GAGTTGGTTGTTGTCTTTGG+TGG - chr5_1:47823979-47823998 None:intergenic 45.0%
! GCAGAGTTGGTTGTTGTCTT+TGG - chr5_1:47823982-47824001 None:intergenic 45.0%
ATGAGAAGAAGGAGCAGAGT+TGG - chr5_1:47823995-47824014 None:intergenic 45.0%
!! GTGATTGGTAACGGGAATGA+TGG - chr5_1:47824020-47824039 None:intergenic 45.0%
GTGAAGGGAATCGTTGTGAT+TGG - chr5_1:47824035-47824054 None:intergenic 45.0%
! TAGCAACCATGGGTTGTGAA+GGG - chr5_1:47824050-47824069 None:intergenic 45.0%
! ACACGTTTGGTAGCAACCAT+GGG - chr5_1:47824060-47824079 None:intergenic 45.0%
CAAACGTGTCAGTTGCACTA+CGG + chr5_1:47824071-47824090 Msa0734170:CDS 45.0%
CAGCAGCAAGTCTTCATCTA+GGG - chr5_1:47824154-47824173 None:intergenic 45.0%
TCAGCAGCAAGTCTTCATCT+AGG - chr5_1:47824155-47824174 None:intergenic 45.0%
AATTTCACCACCCTTCTCAG+GGG - chr5_1:47824300-47824319 None:intergenic 45.0%
CAATTTCACCACCCTTCTCA+GGG - chr5_1:47824301-47824320 None:intergenic 45.0%
ACAATTTCACCACCCTTCTC+AGG - chr5_1:47824302-47824321 None:intergenic 45.0%
!! TAATCAGTGGCGGATCTACT+TGG + chr5_1:47824443-47824462 Msa0734170:intron 45.0%
AGCTCGCACAATTTCTACGA+GGG - chr5_1:47824902-47824921 None:intergenic 45.0%
AAGCTCGCACAATTTCTACG+AGG - chr5_1:47824903-47824922 None:intergenic 45.0%
ACCGTTCAAAGAATGTGCGT+TGG + chr5_1:47825151-47825170 Msa0734170:intron 45.0%
TTGATTGTTGGTGCAGTTGG+AGG + chr5_1:47825304-47825323 Msa0734170:intron 45.0%
!! CGGATTAGTAGATCAGGGTA+AGG - chr5_1:47825830-47825849 None:intergenic 45.0%
TTAAGGAAGGAGTCCTCAAG+GGG + chr5_1:47826076-47826095 Msa0734170:three_prime_UTR 45.0%
GAATCAACCAAAACCCCTTG+AGG - chr5_1:47826092-47826111 None:intergenic 45.0%
GCTTATGTTGGGTTCTTGAG+TGG + chr5_1:47826432-47826451 Msa0734170:three_prime_UTR 45.0%
!!! AATGATTTTTTTTTTTTTTT+TGG + chr5_1:47825271-47825290 Msa0734170:intron 5.0%
ACGATTCCCTTCACAACCCA+TGG + chr5_1:47824041-47824060 Msa0734170:CDS 50.0%
! GTAGCAACCATGGGTTGTGA+AGG - chr5_1:47824051-47824070 None:intergenic 50.0%
! GACACGTTTGGTAGCAACCA+TGG - chr5_1:47824061-47824080 None:intergenic 50.0%
CGTAGTGCAACTGACACGTT+TGG - chr5_1:47824073-47824092 None:intergenic 50.0%
CACGACACTTGAGTATGAGG+CGG + chr5_1:47824113-47824132 Msa0734170:CDS 50.0%
ACACTTGAGTATGAGGCGGA+AGG + chr5_1:47824117-47824136 Msa0734170:CDS 50.0%
!! TGAAGACTTGCTGCTGATGC+CGG + chr5_1:47824158-47824177 Msa0734170:CDS 50.0%
ACAATCACCGCCACATGCTT+TGG - chr5_1:47824207-47824226 None:intergenic 50.0%
AAAGCATGTGGCGGTGATTG+TGG + chr5_1:47824206-47824225 Msa0734170:CDS 50.0%
TGATTGTGGATGGACACAGG+AGG + chr5_1:47824220-47824239 Msa0734170:CDS 50.0%
GCTACATATCCCTCTAACGG+CGG - chr5_1:47824267-47824286 None:intergenic 50.0%
AAACTGGGTTCTTCGTCCGA+AGG + chr5_1:47824371-47824390 Msa0734170:CDS 50.0%
AATGTTATTCGCAGGGGCGA+AGG + chr5_1:47824506-47824525 Msa0734170:intron 50.0%
ATGTTATTCGCAGGGGCGAA+GGG + chr5_1:47824507-47824526 Msa0734170:intron 50.0%
CTCGCACAATTTCTACGAGG+GGG - chr5_1:47824900-47824919 None:intergenic 50.0%
GCTCGCACAATTTCTACGAG+GGG - chr5_1:47824901-47824920 None:intergenic 50.0%
!! CGGTCCGGATTAGTAGATCA+GGG - chr5_1:47825835-47825854 None:intergenic 50.0%
CCTGATCTACTAATCCGGAC+CGG + chr5_1:47825833-47825852 Msa0734170:CDS 50.0%
GCTAACTCGAAGTTCTCCAC+CGG - chr5_1:47825855-47825874 None:intergenic 50.0%
CAGAGAGAAAGACGTTTCGG+TGG + chr5_1:47825980-47825999 Msa0734170:CDS 50.0%
AGAGAAAGACGTTTCGGTGG+TGG + chr5_1:47825983-47826002 Msa0734170:CDS 50.0%
GAGAAAGACGTTTCGGTGGT+GGG + chr5_1:47825984-47826003 Msa0734170:CDS 50.0%
! AAGGAGTCCTCAAGGGGTTT+TGG + chr5_1:47826082-47826101 Msa0734170:three_prime_UTR 50.0%
GGAGCTGATGCCAAAGCATG+TGG + chr5_1:47824194-47824213 Msa0734170:CDS 55.0%
GCTGATGCCAAAGCATGTGG+CGG + chr5_1:47824197-47824216 Msa0734170:CDS 55.0%
CATGTGGCGGTGATTGTGGA+TGG + chr5_1:47824210-47824229 Msa0734170:CDS 55.0%
CGGTGATTGTGGATGGACAC+AGG + chr5_1:47824217-47824236 Msa0734170:CDS 55.0%
TTGTGGATGGACACAGGAGG+TGG + chr5_1:47824223-47824242 Msa0734170:CDS 55.0%
TGTGGATGGACACAGGAGGT+GGG + chr5_1:47824224-47824243 Msa0734170:CDS 55.0%
CTTAGTGCAGCCCCTGAGAA+GGG + chr5_1:47824287-47824306 Msa0734170:CDS 55.0%
TCATGGCCATGGGGTAGCAA+TGG + chr5_1:47824532-47824551 Msa0734170:intron 55.0%
!! CCGGTCCGGATTAGTAGATC+AGG - chr5_1:47825836-47825855 None:intergenic 55.0%
GATCTACTAATCCGGACCGG+TGG + chr5_1:47825836-47825855 Msa0734170:CDS 55.0%
AGCTCCTCCTTCAGCTCTGC+CGG - chr5_1:47824180-47824199 None:intergenic 60.0%
AGGAGGTGGGCGAAAACGAG+AGG + chr5_1:47824237-47824256 Msa0734170:CDS 60.0%
GGAGGTGGGCGAAAACGAGA+GGG + chr5_1:47824238-47824257 Msa0734170:CDS 60.0%
AGAGGGTTGCCGCCGTTAGA+GGG + chr5_1:47824255-47824274 Msa0734170:CDS 60.0%
CCTTCTCAGGGGCTGCACTA+AGG - chr5_1:47824289-47824308 None:intergenic 60.0%
CCTTAGTGCAGCCCCTGAGA+AGG + chr5_1:47824286-47824305 Msa0734170:CDS 60.0%
GCGAAGGGCTTCATGGCCAT+GGG + chr5_1:47824522-47824541 Msa0734170:intron 60.0%
CGAAGGGCTTCATGGCCATG+GGG + chr5_1:47824523-47824542 Msa0734170:intron 60.0%
GGGTAGCCATTGCTACCCCA+TGG - chr5_1:47824541-47824560 None:intergenic 60.0%
AATTGTGCGAGCTTCGCCGC+TGG + chr5_1:47824909-47824928 Msa0734170:intron 60.0%
CTCGAAGTTCTCCACCGGTC+CGG - chr5_1:47825850-47825869 None:intergenic 60.0%
GCTGATGCCGGCAGAGCTGA+AGG + chr5_1:47824170-47824189 Msa0734170:CDS 65.0%
GATGCCGGCAGAGCTGAAGG+AGG + chr5_1:47824173-47824192 Msa0734170:CDS 65.0%
GAGAGGGTTGCCGCCGTTAG+AGG + chr5_1:47824254-47824273 Msa0734170:CDS 65.0%
AGTGCAGCCCCTGAGAAGGG+TGG + chr5_1:47824290-47824309 Msa0734170:CDS 65.0%
GGCGAAGGGCTTCATGGCCA+TGG + chr5_1:47824521-47824540 Msa0734170:intron 65.0%
CGCAGGGGCGAAGGGCTTCA+TGG + chr5_1:47824515-47824534 Msa0734170:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 47823917 47826576 47823917 ID=Msa0734170;Name=Msa0734170
chr5_1 mRNA 47823917 47826576 47823917 ID=Msa0734170-mRNA-1;Parent=Msa0734170;Name=Msa0734170-mRNA-1;_AED=0.02;_eAED=0.02;_QI=5|1|1|1|1|1|3|561|328
chr5_1 exon 47823917 47824392 47823917 ID=Msa0734170-mRNA-1:exon:13694;Parent=Msa0734170-mRNA-1
chr5_1 exon 47825320 47825387 47825320 ID=Msa0734170-mRNA-1:exon:13695;Parent=Msa0734170-mRNA-1
chr5_1 exon 47825568 47826576 47825568 ID=Msa0734170-mRNA-1:exon:13696;Parent=Msa0734170-mRNA-1
chr5_1 five_prime_UTR 47823917 47823921 47823917 ID=Msa0734170-mRNA-1:five_prime_utr;Parent=Msa0734170-mRNA-1
chr5_1 CDS 47823922 47824392 47823922 ID=Msa0734170-mRNA-1:cds;Parent=Msa0734170-mRNA-1
chr5_1 CDS 47825320 47825387 47825320 ID=Msa0734170-mRNA-1:cds;Parent=Msa0734170-mRNA-1
chr5_1 CDS 47825568 47826015 47825568 ID=Msa0734170-mRNA-1:cds;Parent=Msa0734170-mRNA-1
chr5_1 three_prime_UTR 47826016 47826576 47826016 ID=Msa0734170-mRNA-1:three_prime_utr;Parent=Msa0734170-mRNA-1
Gene Sequence

>Msa0734170

ATGTTCTCGTTGAGTCTCCCCATTGCCCTAAAAAACACTCTAATAACTTGTCCACCACCAAAGACAACAACCAACTCTGCTCCTTCTTCTCATTACCATCATTCCCGTTACCAATCACAACGATTCCCTTCACAACCCATGGTTGCTACCAAACGTGTCAGTTGCACTACGGCTGCTGCTCCGATTCCAAACACGACACTTGAGTATGAGGCGGAAGGAAGATCTTCTTCCCTAGATGAAGACTTGCTGCTGATGCCGGCAGAGCTGAAGGAGGAGCTGATGCCAAAGCATGTGGCGGTGATTGTGGATGGACACAGGAGGTGGGCGAAAACGAGAGGGTTGCCGCCGTTAGAGGGATATGTAGCCTTAGTGCAGCCCCTGAGAAGGGTGGTGAAATTGTGTTTAAGCTGGGGAATAAAGATTTTCACTGTTTTCGTGTTGTCTACTGAAAACTGGGTTCTTCGTCCGAAGTTGGAGGTTGAATTCTTGTTAAGCCTCTTTGAAAGAACAATCAATTGTGAAATTGAAGCAATCATGAGAGAAAGAATTAAAATATCTGTGATTGGTGATTCATCAAAGTTCCCCAAGTCTCTACAGAAAATGATAGCTTATATTGAGGAGAAGACAAAGGATAATTCAAGACTCCATCTTATTGTGGCAATTAACTATGGAGGAAGATATGATGTAGTCAAAGCATGTAAAAGCGTAGCTAAGAAAGTGCAAGATGGAATTATTCATCTAGAAGACATTGATGAAAACATTATTGGAAATGATTTGGAGACTAAATGTATCGAGTTCCCTTACCCTGATCTACTAATCCGGACCGGTGGAGAACTTCGAGTTAGCAACTTCTTGTTATGGCAATTGGCATATACAGAACTTTTCTTTAATCGAAAACTTTGGCCAGATTTTGAGAAAAATGATTTTGTTGAGGCCTTAAGTTCATTTCAACAGAGAGAAAGACGTTTCGGTGGTGGGCAACATTAG
Protein sequence

>Msa0734170

MFSLSLPIALKNTLITCPPPKTTTNSAPSSHYHHSRYQSQRFPSQPMVATKRVSCTTAAAPIPNTTLEYEAEGRSSSLDEDLLLMPAELKEELMPKHVAVIVDGHRRWAKTRGLPPLEGYVALVQPLRRVVKLCLSWGIKIFTVFVLSTENWVLRPKLEVEFLLSLFERTINCEIEAIMRERIKISVIGDSSKFPKSLQKMIAYIEEKTKDNSRLHLIVAINYGGRYDVVKACKSVAKKVQDGIIHLEDIDENIIGNDLETKCIEFPYPDLLIRTGGELRVSNFLLWQLAYTELFFNRKLWPDFEKNDFVEALSSFQQRERRFGGGQH*