AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0735250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735250 MtrunA17_Chr5g0432341 85.075 268 13 1 1 241 55 322 6.40e-165 458
Msa0735250 MtrunA17_Chr5g0432351 84.758 269 14 1 1 242 55 323 9.39e-165 458
Msa0735250 MtrunA17_Chr5g0432331 78.358 268 31 1 1 241 86 353 5.60e-154 432
Msa0735250 MtrunA17_Chr5g0432401 69.874 239 60 1 1 239 59 285 4.40e-121 346
Msa0735250 MtrunA17_Chr3g0107881 60.075 268 79 2 1 240 57 324 2.25e-104 305
Msa0735250 MtrunA17_Chr4g0013171 57.249 269 82 5 1 240 57 321 2.05e-95 282
Msa0735250 MtrunA17_Chr4g0013181 56.877 269 83 5 1 240 89 353 1.02e-93 279
Msa0735250 MtrunA17_Chr4g0013201 54.478 268 89 6 1 240 76 338 5.89e-89 266
Msa0735250 MtrunA17_Chr2g0320061 52.985 268 94 5 1 240 55 318 7.65e-88 263
Msa0735250 MtrunA17_Chr2g0320071 53.358 268 92 7 1 240 55 317 1.85e-87 261
Msa0735250 MtrunA17_Chr3g0114451 53.358 268 92 5 1 240 56 318 2.27e-87 261
Msa0735250 MtrunA17_Chr5g0432441 54.412 272 87 8 1 240 60 326 4.77e-87 261
Msa0735250 MtrunA17_Chr7g0244461 51.493 268 97 5 1 240 50 312 5.16e-86 258
Msa0735250 MtrunA17_Chr3g0106921 53.506 271 90 8 1 240 55 320 7.97e-86 258
Msa0735250 MtrunA17_Chr2g0320101 52.985 268 93 7 1 240 55 317 1.70e-85 256
Msa0735250 MtrunA17_Chr2g0320031 51.493 268 98 5 1 240 56 319 1.93e-85 256
Msa0735250 MtrunA17_Chr2g0320051 52.239 268 96 5 1 240 55 318 5.99e-85 255
Msa0735250 MtrunA17_Chr3g0129051 52.593 270 93 6 1 240 58 322 8.53e-85 255
Msa0735250 MtrunA17_Chr7g0244451 52.239 268 95 5 1 240 54 316 1.60e-84 254
Msa0735250 MtrunA17_Chr4g0013191 51.481 270 96 6 1 240 76 340 9.01e-84 253
Msa0735250 MtrunA17_Chr5g0438021 51.481 270 96 6 1 240 61 325 1.26e-83 252
Msa0735250 MtrunA17_Chr1g0213061 52.222 270 94 7 1 240 57 321 1.38e-83 252
Msa0735250 MtrunA17_Chr2g0320091 51.301 269 97 7 1 240 55 318 4.28e-82 248
Msa0735250 MtrunA17_Chr3g0129061 52.963 270 92 6 1 240 58 322 1.07e-78 239
Msa0735250 MtrunA17_Chr7g0224371 47.940 267 107 6 1 240 61 322 3.02e-77 236
Msa0735250 MtrunA17_Chr3g0129041 52.963 270 92 6 1 240 58 322 8.59e-77 234
Msa0735250 MtrunA17_Chr3g0106901 51.119 268 98 5 1 240 61 323 8.85e-76 232
Msa0735250 MtrunA17_Chr5g0404721 46.909 275 107 6 1 240 60 330 3.89e-73 226
Msa0735250 MtrunA17_Chr4g0043391 47.059 272 108 7 1 240 57 324 1.54e-72 224
Msa0735250 MtrunA17_Chr4g0038171 47.426 272 107 7 1 240 54 321 2.18e-72 223
Msa0735250 MtrunA17_Chr8g0372601 46.182 275 109 6 1 240 60 330 2.26e-71 221
Msa0735250 MtrunA17_Chr4g0038161 45.956 272 110 7 1 240 54 320 2.09e-69 216
Msa0735250 MtrunA17_Chr4g0038151 45.956 272 110 7 1 240 55 321 4.91e-69 215
Msa0735250 MtrunA17_Chr2g0292251 44.404 277 116 5 1 242 58 331 5.52e-68 213
Msa0735250 MtrunA17_Chr2g0292271 43.682 277 118 5 1 242 59 332 1.99e-67 212
Msa0735250 MtrunA17_Chr2g0291751 44.928 276 114 6 1 241 59 331 2.88e-67 211
Msa0735250 MtrunA17_Chr1g0155611 43.636 275 117 5 1 240 76 347 3.44e-67 211
Msa0735250 MtrunA17_Chr2g0292091 43.273 275 118 6 1 240 54 325 6.18e-67 210
Msa0735250 MtrunA17_Chr2g0292181 45.455 275 112 6 1 240 68 339 2.52e-66 209
Msa0735250 MtrunA17_Chr4g0070951 45.683 278 109 7 1 241 58 330 5.83e-66 207
Msa0735250 MtrunA17_Chr1g0175561 42.754 276 119 5 1 240 54 326 1.59e-65 206
Msa0735250 MtrunA17_Chr5g0402571 47.653 277 101 11 1 240 57 326 2.19e-65 206
Msa0735250 MtrunA17_Chr2g0292211 43.636 275 117 5 1 240 70 341 1.30e-64 205
Msa0735250 MtrunA17_Chr5g0402581 46.209 277 105 11 1 240 57 326 1.83e-64 203
Msa0735250 MtrunA17_Chr7g0258811 43.116 276 118 7 1 240 99 371 2.33e-64 204
Msa0735250 MtrunA17_Chr2g0292241 43.636 275 117 5 1 240 59 330 6.71e-64 202
Msa0735250 MtrunA17_Chr5g0421411 44.043 277 114 9 2 240 12 285 1.87e-62 197
Msa0735250 MtrunA17_Chr2g0292261 42.909 275 119 5 3 242 61 332 3.81e-62 198
Msa0735250 MtrunA17_Chr2g0292111 42.029 276 122 5 1 241 59 331 7.88e-62 197
Msa0735250 MtrunA17_Chr2g0299691 43.416 281 110 10 1 240 86 358 2.10e-61 196
Msa0735250 MtrunA17_Chr2g0292101 42.960 277 120 6 1 242 55 328 2.47e-61 196
Msa0735250 MtrunA17_Chr4g0068621 41.392 273 123 5 1 240 68 336 2.48e-61 196
Msa0735250 MtrunA17_Chr2g0292291 42.701 274 118 6 3 240 62 332 2.88e-60 193
Msa0735250 MtrunA17_Chr4g0013821 42.647 272 115 6 3 240 60 324 9.76e-60 192
Msa0735250 MtrunA17_Chr2g0292151 42.391 276 121 6 1 241 59 331 2.44e-59 191
Msa0735250 MtrunA17_Chr4g0013721 42.647 272 115 6 3 240 132 396 5.78e-59 192
Msa0735250 MtrunA17_Chr5g0400331 42.500 280 117 7 1 240 56 331 1.10e-57 186
Msa0735250 MtrunA17_Chr3g0128061 40.217 276 124 7 1 240 56 326 2.71e-57 185
Msa0735250 MtrunA17_Chr1g0188431 41.045 268 121 8 1 240 69 327 3.55e-53 174
Msa0735250 MtrunA17_Chr1g0194151 38.628 277 130 5 1 240 60 333 2.60e-52 172
Msa0735250 MtrunA17_Chr1g0164891 38.489 278 129 8 1 240 119 392 3.89e-51 171
Msa0735250 MtrunA17_Chr1g0194271 38.380 284 128 5 1 240 60 340 3.49e-50 167
Msa0735250 MtrunA17_Chr6g0477071 37.313 268 131 8 1 240 84 342 1.14e-48 163
Msa0735250 MtrunA17_Chr1g0204011 39.560 273 121 11 3 240 59 322 2.17e-48 162
Msa0735250 MtrunA17_Chr4g0024121 37.681 276 130 9 1 240 57 326 1.40e-47 160
Msa0735250 MtrunA17_Chr5g0432321 68.966 116 9 1 95 183 17 132 2.68e-47 153
Msa0735250 MtrunA17_Chr2g0322861 37.545 277 129 11 1 240 117 386 5.20e-45 155
Msa0735250 MtrunA17_Chr2g0279041 39.068 279 121 11 1 240 57 325 1.08e-43 150
Msa0735250 MtrunA17_Chr3g0114461 44.503 191 75 4 78 240 5 192 1.38e-42 143
Msa0735250 MtrunA17_Chr7g0254201 35.531 273 131 10 3 241 52 313 1.86e-42 146
Msa0735250 MtrunA17_Chr4g0076211 35.740 277 129 12 1 241 71 334 5.18e-42 145
Msa0735250 MtrunA17_Chr5g0405991 36.264 273 128 9 3 240 55 316 7.43e-41 142
Msa0735250 MtrunA17_Chr7g0225221 37.868 272 129 9 2 240 56 320 1.25e-40 142
Msa0735250 MtrunA17_Chr1g0157051 36.940 268 126 9 3 235 56 315 1.82e-40 141
Msa0735250 MtrunA17_Chr5g0432361 57.143 112 43 1 128 239 2 108 1.88e-40 135
Msa0735250 MtrunA17_Chr6g0460711 33.456 272 141 7 1 240 51 314 1.27e-39 139
Msa0735250 MtrunA17_Chr7g0268501 35.507 276 132 9 1 240 55 320 3.81e-39 138
Msa0735250 MtrunA17_Chr7g0268521 35.507 276 132 9 1 240 55 320 3.81e-39 138
Msa0735250 MtrunA17_Chr8g0371381 35.165 273 131 9 3 240 58 319 1.06e-38 136
Msa0735250 MtrunA17_Chr2g0316791 33.987 306 129 10 1 239 106 405 4.66e-38 137
Msa0735250 MtrunA17_Chr2g0329641 33.692 279 135 10 3 241 68 336 6.20e-38 135
Msa0735250 MtrunA17_Chr2g0316831 33.007 306 132 10 1 239 142 441 3.50e-37 135
Msa0735250 MtrunA17_Chr5g0401431 36.296 270 127 9 6 236 62 325 4.03e-37 132
Msa0735250 MtrunA17_Chr7g0254231 33.582 268 142 6 1 240 52 311 4.46e-37 132
Msa0735250 MtrunA17_Chr2g0296621 36.000 275 128 11 1 240 80 341 9.48e-37 132
Msa0735250 MtrunA17_Chr3g0114491 77.215 79 17 1 1 79 48 125 1.10e-36 126
Msa0735250 MtrunA17_Chr2g0316191 33.550 307 130 11 1 239 106 406 2.29e-36 132
Msa0735250 MtrunA17_Chr2g0279061 38.113 265 115 11 15 240 12 266 3.93e-36 128
Msa0735250 MtrunA17_Chr4g0063671 35.000 280 132 11 1 240 51 320 4.44e-36 130
Msa0735250 MtrunA17_Chr1g0148021 35.379 277 127 12 6 240 62 328 4.52e-36 130
Msa0735250 MtrunA17_Chr4g0072091 36.462 277 122 11 6 240 59 323 6.35e-36 129
Msa0735250 MtrunA17_Chr2g0316481 33.007 306 132 10 1 239 106 405 6.43e-36 131
Msa0735250 MtrunA17_Chr4g0046131 36.000 275 131 10 2 236 73 342 1.85e-35 129
Msa0735250 MtrunA17_Chr6g0467121 37.079 267 126 12 5 240 108 363 2.90e-35 129
Msa0735250 MtrunA17_Chr6g0467161 34.328 268 134 10 5 240 79 336 6.28e-35 127
Msa0735250 MtrunA17_Chr6g0467251 35.361 263 131 9 5 236 70 324 7.15e-35 127
Msa0735250 MtrunA17_Chr1g0197411 33.455 275 140 8 5 241 62 331 2.93e-34 125
Msa0735250 MtrunA17_Chr2g0278671 36.268 284 121 11 2 240 55 323 1.32e-33 123
Msa0735250 MtrunA17_Chr4g0053871 34.962 266 131 8 6 236 62 320 1.70e-33 123
Msa0735250 MtrunA17_Chr8g0364291 35.294 272 134 8 6 240 58 324 2.57e-33 122
Msa0735250 MtrunA17_Chr1g0181511 31.387 274 149 8 2 242 58 325 7.79e-32 119
Msa0735250 MtrunA17_Chr4g0075241 29.044 272 154 6 3 240 61 327 3.80e-31 117
Msa0735250 MtrunA17_Chr2g0311251 32.000 300 135 10 5 239 44 339 6.05e-31 117
Msa0735250 MtrunA17_Chr2g0311241 29.480 346 131 11 1 239 90 429 1.06e-30 117
Msa0735250 MtrunA17_Chr2g0316141 33.333 273 126 11 17 239 11 277 2.95e-30 113
Msa0735250 MtrunA17_Chr2g0316181 33.333 273 126 11 17 239 11 277 2.95e-30 113
Msa0735250 MtrunA17_Chr3g0136751 30.545 275 141 8 5 240 59 322 3.65e-30 114
Msa0735250 MtrunA17_Chr6g0451571 31.541 279 142 9 2 240 65 334 6.61e-30 114
Msa0735250 MtrunA17_Chr1g0181431 30.882 272 149 8 4 242 60 325 5.23e-29 111
Msa0735250 MtrunA17_Chr5g0413051 30.216 278 149 9 3 240 105 377 9.47e-29 111
Msa0735250 MtrunA17_Chr1g0181481 30.882 272 149 8 4 242 60 325 1.54e-28 110
Msa0735250 MtrunA17_Chr2g0292281 35.545 211 91 5 64 240 17 216 5.21e-28 107
Msa0735250 MtrunA17_Chr2g0316811 47.727 132 63 4 1 130 80 207 1.41e-25 102
Msa0735250 MtrunA17_Chr4g0074851 28.309 272 156 7 4 242 65 330 2.19e-23 96.7
Msa0735250 MtrunA17_Chr4g0004591 26.804 291 113 9 4 239 110 355 4.92e-19 84.7
Msa0735250 MtrunA17_Chr3g0104101 39.535 129 67 5 123 240 21 149 4.56e-17 75.5
Msa0735250 MtrunA17_Chr6g0468731 54.795 73 28 3 10 82 22 89 6.26e-17 73.6
Msa0735250 MtrunA17_Chr1g0196191 51.471 68 31 2 13 80 27 92 1.33e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735250 AT5G05340.1 52.222 270 94 6 1 240 60 324 8.90e-86 258
Msa0735250 AT5G58390.1 50.923 271 97 7 1 240 51 316 5.73e-79 240
Msa0735250 AT5G58400.1 49.446 271 101 7 1 240 60 325 1.36e-76 234
Msa0735250 AT2G18150.1 44.727 275 113 6 1 240 66 336 3.43e-72 224
Msa0735250 AT1G14550.1 45.926 270 112 7 1 240 56 321 1.02e-70 219
Msa0735250 AT2G38390.1 44.928 276 113 5 1 240 61 333 1.04e-69 218
Msa0735250 AT5G66390.1 44.928 276 113 6 1 241 63 334 1.15e-69 217
Msa0735250 AT2G18140.1 45.091 275 112 7 1 240 65 335 1.32e-69 217
Msa0735250 AT4G08770.1 45.848 277 110 6 1 240 54 327 8.09e-69 215
Msa0735250 AT1G14540.1 45.353 269 114 7 1 240 51 315 6.80e-68 212
Msa0735250 AT4G36430.1 44.364 275 114 6 1 240 60 330 1.45e-67 212
Msa0735250 AT2G38380.1 44.203 276 115 5 1 240 61 333 8.53e-67 210
Msa0735250 AT5G06720.1 42.909 275 119 6 1 240 62 333 4.49e-66 208
Msa0735250 AT3G49110.1 43.728 279 117 6 1 242 63 338 2.20e-65 207
Msa0735250 AT5G06730.1 42.391 276 119 6 1 240 63 334 1.03e-64 205
Msa0735250 AT4G08780.1 44.043 277 115 6 1 240 54 327 1.05e-64 205
Msa0735250 AT3G49120.1 43.011 279 119 6 1 242 62 337 1.26e-64 205
Msa0735250 AT3G32980.1 43.728 279 117 6 1 242 61 336 1.38e-64 205
Msa0735250 AT3G50990.1 43.682 277 117 8 1 242 71 343 2.20e-63 201
Msa0735250 AT1G44970.1 42.545 275 120 5 1 240 75 346 2.45e-63 201
Msa0735250 AT5G19890.1 44.245 278 107 11 1 240 60 327 8.67e-63 199
Msa0735250 AT1G49570.1 43.796 274 111 9 1 236 78 346 2.78e-62 199
Msa0735250 AT4G16270.1 41.667 276 118 7 1 240 80 348 5.18e-60 193
Msa0735250 AT1G68850.1 41.367 278 121 9 2 240 61 335 7.07e-58 187
Msa0735250 AT2G35380.1 42.652 279 118 6 1 240 60 335 4.60e-55 180
Msa0735250 AT5G19880.1 41.281 281 118 8 1 240 55 329 6.15e-55 179
Msa0735250 AT2G22420.1 38.686 274 125 8 3 241 55 320 1.78e-54 178
Msa0735250 AT1G71695.1 39.852 271 127 6 1 240 74 339 6.83e-54 177
Msa0735250 AT4G33420.1 39.179 268 126 7 1 240 67 325 2.66e-49 164
Msa0735250 AT4G33420.2 39.179 268 126 7 1 240 114 372 9.36e-49 164
Msa0735250 AT5G15180.1 38.321 274 129 7 1 240 62 329 1.88e-48 162
Msa0735250 AT3G01190.1 38.686 274 126 8 1 240 56 321 2.36e-48 162
Msa0735250 AT5G51890.1 38.686 274 123 11 3 240 58 322 1.46e-47 160
Msa0735250 AT3G03670.1 36.264 273 133 7 3 240 55 321 1.77e-47 160
Msa0735250 AT1G05240.1 36.264 273 130 8 1 236 56 321 8.67e-44 150
Msa0735250 AT1G05250.1 36.264 273 130 8 1 236 56 321 8.67e-44 150
Msa0735250 AT5G64120.1 38.321 274 125 10 1 240 65 328 1.78e-42 147
Msa0735250 AT4G11290.1 33.696 276 143 7 1 240 55 326 3.32e-42 146
Msa0735250 AT5G17820.1 35.165 273 127 9 3 240 56 313 2.50e-41 144
Msa0735250 AT3G21770.1 35.294 272 134 9 1 236 59 324 1.06e-40 142
Msa0735250 AT5G17820.2 35.662 272 127 9 3 240 109 366 1.60e-40 143
Msa0735250 AT1G05260.1 34.058 276 140 9 1 240 56 325 2.98e-40 141
Msa0735250 AT5G42180.1 36.029 272 129 9 3 239 55 316 6.89e-40 140
Msa0735250 AT5G64100.1 36.765 272 125 11 5 240 71 331 7.53e-40 140
Msa0735250 AT2G35380.2 39.357 249 109 6 31 240 2 247 9.55e-40 137
Msa0735250 AT5G39580.1 38.376 271 122 11 5 240 59 319 9.61e-39 137
Msa0735250 AT4G26010.1 34.211 266 137 9 3 240 54 309 2.20e-37 133
Msa0735250 AT4G26010.2 34.211 266 137 9 3 240 82 337 2.54e-37 134
Msa0735250 AT1G34510.1 33.208 265 141 8 3 240 54 309 3.26e-37 133
Msa0735250 AT5G22410.1 32.143 280 142 10 1 242 58 327 1.70e-36 131
Msa0735250 AT5G40150.1 33.091 275 139 7 2 240 63 328 2.17e-36 131
Msa0735250 AT4G33870.1 30.744 309 136 10 1 238 98 399 3.69e-36 132
Msa0735250 AT4G37530.1 35.842 279 128 10 4 240 60 329 7.70e-36 130
Msa0735250 AT3G28200.1 32.847 274 140 7 2 240 52 316 1.47e-35 129
Msa0735250 AT2G41480.1 35.145 276 129 11 5 240 63 328 3.37e-35 128
Msa0735250 AT5G67400.1 36.331 278 128 11 4 240 60 329 5.30e-34 125
Msa0735250 AT4G30170.1 34.420 276 132 11 4 240 60 325 8.55e-34 124
Msa0735250 AT5G64110.1 36.264 273 127 11 5 240 68 330 8.94e-34 124
Msa0735250 AT1G30870.1 34.892 278 130 12 1 240 81 345 8.97e-34 125
Msa0735250 AT5G24070.1 32.270 282 140 9 1 242 67 337 1.04e-33 124
Msa0735250 AT2G43480.1 32.500 280 138 10 1 240 67 335 2.23e-33 123
Msa0735250 AT4G17690.1 35.165 273 138 10 2 241 57 323 2.47e-33 123
Msa0735250 AT2G18980.1 34.657 277 126 12 6 240 60 323 3.73e-33 122
Msa0735250 AT3G49960.1 35.714 280 127 12 4 240 60 329 5.99e-33 122
Msa0735250 AT2G39040.1 33.096 281 141 8 1 240 76 350 6.94e-33 122
Msa0735250 AT1G24110.1 31.985 272 142 8 6 240 57 322 4.55e-32 120
Msa0735250 AT5G47000.1 34.066 273 140 10 2 240 64 330 1.23e-31 119
Msa0735250 AT2G34060.1 33.333 273 135 11 6 237 77 343 2.35e-30 115
Msa0735250 AT5G14130.1 32.103 271 141 8 6 240 67 330 4.70e-30 114
Msa0735250 AT4G37520.1 32.847 274 143 7 4 240 60 329 5.63e-30 114
Msa0735250 AT5G14130.2 32.103 271 141 8 6 240 108 371 8.61e-30 114
Msa0735250 AT1G77100.1 31.884 276 140 10 2 240 55 319 2.20e-29 112
Msa0735250 AT4G37520.2 32.482 274 141 8 4 240 60 326 4.71e-28 109
Msa0735250 AT4G21960.1 29.044 272 154 7 4 242 64 329 2.10e-27 107
Msa0735250 AT4G25980.1 31.655 278 145 10 1 240 56 326 2.89e-27 107
Msa0735250 AT4G37530.2 35.841 226 96 9 4 187 60 278 5.27e-27 105
Msa0735250 AT3G17070.1 29.091 275 153 6 3 240 70 339 5.47e-27 106
Msa0735250 AT2G24800.1 29.091 275 146 8 5 240 65 329 2.16e-25 102
Msa0735250 AT4G31760.1 28.938 273 148 8 5 240 63 326 1.61e-24 99.8
Msa0735250 AT4G25980.2 51.546 97 41 4 1 97 101 191 1.50e-23 96.3
Msa0735250 AT5G39580.2 54.128 109 42 6 5 113 59 159 7.12e-23 93.6
Msa0735250 AT3G17070.2 27.170 265 150 7 13 240 18 276 2.19e-20 87.8
Msa0735250 AT2G24800.2 39.024 123 68 3 5 127 65 180 5.17e-17 77.8

Find 33 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTAATTGAATTGTTATT+TGG 0.117052 5_1:+50383945 None:intergenic
CCAAATAACAATTCAATTAG+AGG 0.210718 5_1:-50383945 Msa0735250:CDS
GAAGCCTCTTACTGGGAAAA+AGG 0.220866 5_1:-50383050 Msa0735250:CDS
TTACAATCGCCGCCGATTCT+AGG 0.235834 5_1:+50383277 None:intergenic
TTGCAAATTTGCTGCAAAAT+TGG 0.238319 5_1:+50383309 None:intergenic
GGTAATTTGAAGCCTCTTAC+TGG 0.288056 5_1:-50383058 Msa0735250:CDS
GTAACACTTGGTACCAATAT+TGG 0.304974 5_1:+50383542 None:intergenic
AGTTGACACATCCTATTATA+AGG 0.341339 5_1:-50383224 Msa0735250:CDS
AACATCACGTTTGCAAGCTT+TGG 0.348583 5_1:+50383887 None:intergenic
ATACAACAAAGCCTTATAAT+AGG 0.352068 5_1:+50383213 None:intergenic
TAGGATGTGTCAACTTTGTT+AGG 0.364639 5_1:+50383232 None:intergenic
AGATGGGTGGTAACCAATAT+TGG 0.389155 5_1:-50383555 Msa0735250:CDS
GATGACACTCCTACATTCAT+TGG 0.405241 5_1:-50383984 Msa0735250:CDS
TGGAGTTTCTATGATTAAGA+TGG 0.424465 5_1:-50383080 Msa0735250:CDS
TACAATCGCCGCCGATTCTA+GGG 0.435646 5_1:+50383278 None:intergenic
TATTGGTACCAAGTGTTACT+AGG 0.448839 5_1:-50383538 Msa0735250:CDS
AGCTGCTATAGCTAAAATAT+CGG 0.458807 5_1:+50383854 None:intergenic
GGAGTTTCTATGATTAAGAT+GGG 0.461747 5_1:-50383079 Msa0735250:CDS
CTCTTCTTCCTAGTAACACT+TGG 0.476428 5_1:+50383530 None:intergenic
TGCAAAATTGGTGTCTATGT+TGG 0.481455 5_1:+50383321 None:intergenic
CAAAAGACATGCCCTAGAAT+CGG 0.510123 5_1:-50383289 Msa0735250:CDS
TTGTTAGGTGTAGAATCAAA+TGG 0.518388 5_1:+50383247 None:intergenic
GTAATTTGAAGCCTCTTACT+GGG 0.519840 5_1:-50383057 Msa0735250:CDS
TTACGCTAACAAAGCAGTCA+TGG 0.539113 5_1:+50384174 None:intergenic
ATATCTACGCAGGGTTGTGA+TGG 0.543434 5_1:-50384020 Msa0735250:intron
AGCTATAGCAGCTCGAGACT+CGG 0.543944 5_1:-50383844 Msa0735250:CDS
AAAGCTCTTGATCAGAATGA+AGG 0.549289 5_1:+50383179 None:intergenic
TACGCAGGGTTGTGATGGAT+CGG 0.567173 5_1:-50384015 Msa0735250:intron
TGGTTCTCAAAGTGATAGAT+TGG 0.568189 5_1:-50383146 Msa0735250:CDS
GTCTTCTCACCAATGAATGT+AGG 0.594677 5_1:+50383975 None:intergenic
AAGGCTTTGTTGTATAAACA+AGG 0.598286 5_1:-50383205 Msa0735250:CDS
TTCTTCAATCAACAGATGGG+TGG 0.637118 5_1:-50383568 Msa0735250:intron
AAGACATGCCCTAGAATCGG+CGG 0.670665 5_1:-50383286 Msa0735250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTATATCTTTTTATATTG+CGG + chr5_1:50383470-50383489 None:intergenic 15.0%
!!! ATATGAGTTTAATTTAAACA+TGG + chr5_1:50383560-50383579 None:intergenic 15.0%
!! AACTCATATTTAATATTTGT+TGG - chr5_1:50383571-50383590 Msa0735250:intron 15.0%
!!! AAGTATATCTTTTTATATTG+CGG + chr5_1:50383470-50383489 None:intergenic 15.0%
!!! ATATGAGTTTAATTTAAACA+TGG + chr5_1:50383560-50383579 None:intergenic 15.0%
!! AACTCATATTTAATATTTGT+TGG - chr5_1:50383571-50383590 Msa0735250:intron 15.0%
!!! GATTAAAAAGTGCAAAATAT+TGG + chr5_1:50383415-50383434 None:intergenic 20.0%
!!! TATTTTGCACTTTTTAATCA+TGG - chr5_1:50383416-50383435 Msa0735250:intron 20.0%
!! ACTTGTATTGTAGAAAAATT+TGG + chr5_1:50383501-50383520 None:intergenic 20.0%
!!! GATTAAAAAGTGCAAAATAT+TGG + chr5_1:50383415-50383434 None:intergenic 20.0%
!!! TATTTTGCACTTTTTAATCA+TGG - chr5_1:50383416-50383435 Msa0735250:intron 20.0%
!! ACTTGTATTGTAGAAAAATT+TGG + chr5_1:50383501-50383520 None:intergenic 20.0%
!! CCTCTAATTGAATTGTTATT+TGG + chr5_1:50383270-50383289 None:intergenic 25.0%
! CCAAATAACAATTCAATTAG+AGG - chr5_1:50383267-50383286 Msa0735250:CDS 25.0%
! ATTAGGAAAATCCAATTGTA+TGG + chr5_1:50383838-50383857 None:intergenic 25.0%
!! ATACAACAAAGCCTTATAAT+AGG + chr5_1:50384002-50384021 None:intergenic 25.0%
!! TATGCTTTTGCTAAAGATTT+TGG - chr5_1:50384112-50384131 Msa0735250:intron 25.0%
!! CCTCTAATTGAATTGTTATT+TGG + chr5_1:50383270-50383289 None:intergenic 25.0%
! CCAAATAACAATTCAATTAG+AGG - chr5_1:50383267-50383286 Msa0735250:CDS 25.0%
! ATTAGGAAAATCCAATTGTA+TGG + chr5_1:50383838-50383857 None:intergenic 25.0%
!! ATACAACAAAGCCTTATAAT+AGG + chr5_1:50384002-50384021 None:intergenic 25.0%
!! TATGCTTTTGCTAAAGATTT+TGG - chr5_1:50384112-50384131 Msa0735250:intron 25.0%
! CAATTCAATTAGAGGTTTTG+AGG - chr5_1:50383275-50383294 Msa0735250:CDS 30.0%
AGCTGCTATAGCTAAAATAT+CGG + chr5_1:50383361-50383380 None:intergenic 30.0%
!!! ATGGTTTTAAGTTAGAGTTG+CGG + chr5_1:50383541-50383560 None:intergenic 30.0%
ATGTTTCTTCAATCAACAGA+TGG - chr5_1:50383640-50383659 Msa0735250:intron 30.0%
TGTTTCTTCAATCAACAGAT+GGG - chr5_1:50383641-50383660 Msa0735250:intron 30.0%
AGTTGTGAGAAGTTGAAAAA+TGG + chr5_1:50383755-50383774 None:intergenic 30.0%
! ATCACAAATTTCAAGTCACA+TGG - chr5_1:50383776-50383795 Msa0735250:intron 30.0%
ATAGAACAACCAAGTCTTTA+AGG + chr5_1:50383808-50383827 None:intergenic 30.0%
!! GTTTCTAAAGTTAGTGCATT+AGG + chr5_1:50383855-50383874 None:intergenic 30.0%
!! TTGCAAATTTGCTGCAAAAT+TGG + chr5_1:50383906-50383925 None:intergenic 30.0%
TTGTTAGGTGTAGAATCAAA+TGG + chr5_1:50383968-50383987 None:intergenic 30.0%
AGTTGACACATCCTATTATA+AGG - chr5_1:50383988-50384007 Msa0735250:CDS 30.0%
!! AAGGCTTTGTTGTATAAACA+AGG - chr5_1:50384007-50384026 Msa0735250:CDS 30.0%
TGGAGTTTCTATGATTAAGA+TGG - chr5_1:50384132-50384151 Msa0735250:intron 30.0%
GGAGTTTCTATGATTAAGAT+GGG - chr5_1:50384133-50384152 Msa0735250:intron 30.0%
! CAATTCAATTAGAGGTTTTG+AGG - chr5_1:50383275-50383294 Msa0735250:CDS 30.0%
AGCTGCTATAGCTAAAATAT+CGG + chr5_1:50383361-50383380 None:intergenic 30.0%
!!! ATGGTTTTAAGTTAGAGTTG+CGG + chr5_1:50383541-50383560 None:intergenic 30.0%
ATGTTTCTTCAATCAACAGA+TGG - chr5_1:50383640-50383659 Msa0735250:intron 30.0%
TGTTTCTTCAATCAACAGAT+GGG - chr5_1:50383641-50383660 Msa0735250:intron 30.0%
AGTTGTGAGAAGTTGAAAAA+TGG + chr5_1:50383755-50383774 None:intergenic 30.0%
! ATCACAAATTTCAAGTCACA+TGG - chr5_1:50383776-50383795 Msa0735250:intron 30.0%
ATAGAACAACCAAGTCTTTA+AGG + chr5_1:50383808-50383827 None:intergenic 30.0%
!! GTTTCTAAAGTTAGTGCATT+AGG + chr5_1:50383855-50383874 None:intergenic 30.0%
!! TTGCAAATTTGCTGCAAAAT+TGG + chr5_1:50383906-50383925 None:intergenic 30.0%
TTGTTAGGTGTAGAATCAAA+TGG + chr5_1:50383968-50383987 None:intergenic 30.0%
AGTTGACACATCCTATTATA+AGG - chr5_1:50383988-50384007 Msa0735250:CDS 30.0%
!! AAGGCTTTGTTGTATAAACA+AGG - chr5_1:50384007-50384026 Msa0735250:CDS 30.0%
TGGAGTTTCTATGATTAAGA+TGG - chr5_1:50384132-50384151 Msa0735250:intron 30.0%
GGAGTTTCTATGATTAAGAT+GGG - chr5_1:50384133-50384152 Msa0735250:intron 30.0%
ATTGGAAAGAGTCCTAACAA+TGG + chr5_1:50383397-50383416 None:intergenic 35.0%
TTATATTGCGGTCGCAATTA+GGG + chr5_1:50383458-50383477 None:intergenic 35.0%
TTTATATTGCGGTCGCAATT+AGG + chr5_1:50383459-50383478 None:intergenic 35.0%
GTAACACTTGGTACCAATAT+TGG + chr5_1:50383673-50383692 None:intergenic 35.0%
! TATTGGTACCAAGTGTTACT+AGG - chr5_1:50383674-50383693 Msa0735250:intron 35.0%
TGTGAGAAGTTGAAAAATGG+AGG + chr5_1:50383752-50383771 None:intergenic 35.0%
TGGACTAAACCTTAAAGACT+TGG - chr5_1:50383796-50383815 Msa0735250:intron 35.0%
! AAAGACTTGGTTGTTCTATC+TGG - chr5_1:50383809-50383828 Msa0735250:intron 35.0%
! TGCAAAATTGGTGTCTATGT+TGG + chr5_1:50383894-50383913 None:intergenic 35.0%
TAGGATGTGTCAACTTTGTT+AGG + chr5_1:50383983-50384002 None:intergenic 35.0%
AAAGCTCTTGATCAGAATGA+AGG + chr5_1:50384036-50384055 None:intergenic 35.0%
! TCTGATCAAGAGCTTTTCAA+AGG - chr5_1:50384040-50384059 Msa0735250:intron 35.0%
TGGTTCTCAAAGTGATAGAT+TGG - chr5_1:50384066-50384085 Msa0735250:intron 35.0%
! GTAATTTGAAGCCTCTTACT+GGG - chr5_1:50384155-50384174 Msa0735250:intron 35.0%
ATTGGAAAGAGTCCTAACAA+TGG + chr5_1:50383397-50383416 None:intergenic 35.0%
TTATATTGCGGTCGCAATTA+GGG + chr5_1:50383458-50383477 None:intergenic 35.0%
TTTATATTGCGGTCGCAATT+AGG + chr5_1:50383459-50383478 None:intergenic 35.0%
GTAACACTTGGTACCAATAT+TGG + chr5_1:50383673-50383692 None:intergenic 35.0%
! TATTGGTACCAAGTGTTACT+AGG - chr5_1:50383674-50383693 Msa0735250:intron 35.0%
TGTGAGAAGTTGAAAAATGG+AGG + chr5_1:50383752-50383771 None:intergenic 35.0%
TGGACTAAACCTTAAAGACT+TGG - chr5_1:50383796-50383815 Msa0735250:intron 35.0%
! AAAGACTTGGTTGTTCTATC+TGG - chr5_1:50383809-50383828 Msa0735250:intron 35.0%
! TGCAAAATTGGTGTCTATGT+TGG + chr5_1:50383894-50383913 None:intergenic 35.0%
TAGGATGTGTCAACTTTGTT+AGG + chr5_1:50383983-50384002 None:intergenic 35.0%
AAAGCTCTTGATCAGAATGA+AGG + chr5_1:50384036-50384055 None:intergenic 35.0%
! TCTGATCAAGAGCTTTTCAA+AGG - chr5_1:50384040-50384059 Msa0735250:intron 35.0%
TGGTTCTCAAAGTGATAGAT+TGG - chr5_1:50384066-50384085 Msa0735250:intron 35.0%
! GTAATTTGAAGCCTCTTACT+GGG - chr5_1:50384155-50384174 Msa0735250:intron 35.0%
TTACGCTAACAAAGCAGTCA+TGG + chr5_1:50383041-50383060 None:intergenic 40.0%
ACGTATTGTCATATCTACGC+AGG - chr5_1:50383182-50383201 Msa0735250:CDS 40.0%
CGTATTGTCATATCTACGCA+GGG - chr5_1:50383183-50383202 Msa0735250:CDS 40.0%
GATGACACTCCTACATTCAT+TGG - chr5_1:50383228-50383247 Msa0735250:CDS 40.0%
GTCTTCTCACCAATGAATGT+AGG + chr5_1:50383240-50383259 None:intergenic 40.0%
AACATCACGTTTGCAAGCTT+TGG + chr5_1:50383328-50383347 None:intergenic 40.0%
TTCTTCAATCAACAGATGGG+TGG - chr5_1:50383644-50383663 Msa0735250:intron 40.0%
AGATGGGTGGTAACCAATAT+TGG - chr5_1:50383657-50383676 Msa0735250:intron 40.0%
CTCTTCTTCCTAGTAACACT+TGG + chr5_1:50383685-50383704 None:intergenic 40.0%
GAGAAGTTGAAAAATGGAGG+AGG + chr5_1:50383749-50383768 None:intergenic 40.0%
CAAAAGACATGCCCTAGAAT+CGG - chr5_1:50383923-50383942 Msa0735250:CDS 40.0%
! CAAGAGCTTTTCAAAGGTGA+TGG - chr5_1:50384046-50384065 Msa0735250:intron 40.0%
! GGTAATTTGAAGCCTCTTAC+TGG - chr5_1:50384154-50384173 Msa0735250:intron 40.0%
AAGCCTCTTACTGGGAAAAA+GGG - chr5_1:50384163-50384182 Msa0735250:intron 40.0%
TTACGCTAACAAAGCAGTCA+TGG + chr5_1:50383041-50383060 None:intergenic 40.0%
ACGTATTGTCATATCTACGC+AGG - chr5_1:50383182-50383201 Msa0735250:CDS 40.0%
CGTATTGTCATATCTACGCA+GGG - chr5_1:50383183-50383202 Msa0735250:CDS 40.0%
GATGACACTCCTACATTCAT+TGG - chr5_1:50383228-50383247 Msa0735250:CDS 40.0%
GTCTTCTCACCAATGAATGT+AGG + chr5_1:50383240-50383259 None:intergenic 40.0%
AACATCACGTTTGCAAGCTT+TGG + chr5_1:50383328-50383347 None:intergenic 40.0%
TTCTTCAATCAACAGATGGG+TGG - chr5_1:50383644-50383663 Msa0735250:intron 40.0%
AGATGGGTGGTAACCAATAT+TGG - chr5_1:50383657-50383676 Msa0735250:intron 40.0%
CTCTTCTTCCTAGTAACACT+TGG + chr5_1:50383685-50383704 None:intergenic 40.0%
GAGAAGTTGAAAAATGGAGG+AGG + chr5_1:50383749-50383768 None:intergenic 40.0%
CAAAAGACATGCCCTAGAAT+CGG - chr5_1:50383923-50383942 Msa0735250:CDS 40.0%
! CAAGAGCTTTTCAAAGGTGA+TGG - chr5_1:50384046-50384065 Msa0735250:intron 40.0%
! GGTAATTTGAAGCCTCTTAC+TGG - chr5_1:50384154-50384173 Msa0735250:intron 40.0%
AAGCCTCTTACTGGGAAAAA+GGG - chr5_1:50384163-50384182 Msa0735250:intron 40.0%
ATATCTACGCAGGGTTGTGA+TGG - chr5_1:50383192-50383211 Msa0735250:CDS 45.0%
! GACTTGGTTGTTCTATCTGG+TGG - chr5_1:50383812-50383831 Msa0735250:intron 45.0%
CTATCTGGTGGCCATACAAT+TGG - chr5_1:50383824-50383843 Msa0735250:intron 45.0%
GAAGCCTCTTACTGGGAAAA+AGG - chr5_1:50384162-50384181 Msa0735250:intron 45.0%
! TCACCCTTTTTCCCAGTAAG+AGG + chr5_1:50384169-50384188 None:intergenic 45.0%
CTGGGAAAAAGGGTGAGATT+AGG - chr5_1:50384173-50384192 Msa0735250:intron 45.0%
ATATCTACGCAGGGTTGTGA+TGG - chr5_1:50383192-50383211 Msa0735250:CDS 45.0%
! GACTTGGTTGTTCTATCTGG+TGG - chr5_1:50383812-50383831 Msa0735250:intron 45.0%
CTATCTGGTGGCCATACAAT+TGG - chr5_1:50383824-50383843 Msa0735250:intron 45.0%
GAAGCCTCTTACTGGGAAAA+AGG - chr5_1:50384162-50384181 Msa0735250:intron 45.0%
! TCACCCTTTTTCCCAGTAAG+AGG + chr5_1:50384169-50384188 None:intergenic 45.0%
CTGGGAAAAAGGGTGAGATT+AGG - chr5_1:50384173-50384192 Msa0735250:intron 45.0%
!! TACGCAGGGTTGTGATGGAT+CGG - chr5_1:50383197-50383216 Msa0735250:CDS 50.0%
AGCTATAGCAGCTCGAGACT+CGG - chr5_1:50383368-50383387 Msa0735250:intron 50.0%
! GAGACTCGGTTGCCATTGTT+AGG - chr5_1:50383382-50383401 Msa0735250:intron 50.0%
AAGACATGCCCTAGAATCGG+CGG - chr5_1:50383926-50383945 Msa0735250:CDS 50.0%
!! TACAATCGCCGCCGATTCTA+GGG + chr5_1:50383937-50383956 None:intergenic 50.0%
!! TTACAATCGCCGCCGATTCT+AGG + chr5_1:50383938-50383957 None:intergenic 50.0%
!! TACGCAGGGTTGTGATGGAT+CGG - chr5_1:50383197-50383216 Msa0735250:CDS 50.0%
AGCTATAGCAGCTCGAGACT+CGG - chr5_1:50383368-50383387 Msa0735250:intron 50.0%
! GAGACTCGGTTGCCATTGTT+AGG - chr5_1:50383382-50383401 Msa0735250:intron 50.0%
AAGACATGCCCTAGAATCGG+CGG - chr5_1:50383926-50383945 Msa0735250:CDS 50.0%
!! TACAATCGCCGCCGATTCTA+GGG + chr5_1:50383937-50383956 None:intergenic 50.0%
!! TTACAATCGCCGCCGATTCT+AGG + chr5_1:50383938-50383957 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 50383009 50384225 50383009 ID=Msa0735250;Name=Msa0735250
chr5_1 mRNA 50383009 50384225 50383009 ID=Msa0735250-mRNA-1;Parent=Msa0735250;Name=Msa0735250-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|4|0|242
chr5_1 exon 50384178 50384225 50384178 ID=Msa0735250-mRNA-1:exon:14232;Parent=Msa0735250-mRNA-1
chr5_1 exon 50383836 50384030 50383836 ID=Msa0735250-mRNA-1:exon:14231;Parent=Msa0735250-mRNA-1
chr5_1 exon 50383445 50383575 50383445 ID=Msa0735250-mRNA-1:exon:14230;Parent=Msa0735250-mRNA-1
chr5_1 exon 50383009 50383363 50383009 ID=Msa0735250-mRNA-1:exon:14229;Parent=Msa0735250-mRNA-1
chr5_1 CDS 50384178 50384225 50384178 ID=Msa0735250-mRNA-1:cds;Parent=Msa0735250-mRNA-1
chr5_1 CDS 50383836 50384030 50383836 ID=Msa0735250-mRNA-1:cds;Parent=Msa0735250-mRNA-1
chr5_1 CDS 50383445 50383575 50383445 ID=Msa0735250-mRNA-1:cds;Parent=Msa0735250-mRNA-1
chr5_1 CDS 50383009 50383363 50383009 ID=Msa0735250-mRNA-1:cds;Parent=Msa0735250-mRNA-1
Gene Sequence

>Msa0735250

ATGGGAGCATCGCTGCTACGTTTGCATTTCCATGACTGCTTTGTTAGCGGTTGTGATGGATCGGTTCTGCTAGATGACACTCCTACATTCATTGGTGAGAAGACTGCATTTCCAAATAACAATTCAATTAGAGGTTTTGAGGTTGTTGATCAAATTAAAGCTGCAGTTACCAAAGCTTGCAAACGTGATGTTGTATCTTGTGCCGATATTTTAGCTATAGCAGCTCGAGACTCGGTTGCCATTATGGGTGGTAACCAATATTGGTACCAAGTGTTACTAGGAAGAAGAGATTCAATATTTGCAAGCAGAGATGCAGCAAATACAAATCTTCCTCCTCCATTTTTCAACTTCTCACAACTCATCACAAATTTCAAAAACAGAATCTACAATGAAACCAACATAGACACCAATTTTGCAGCAAATTTGCAAAAGACATGCCCTAGAATCGGCGGCGATTGTAATTTAGCACCATTTGATTCTACACCTAACAAAGTTGACACATCCTATTATAAGGCTTTGTTGTATAAACAAGGTCTCCTTCATTCTGATCAAGAGCTTTTCAAAGGTGATGGTTCTCAAAGTGATAGATTGGTGCAACTTTATAGTAAAAATTCTTATGCTTTTGCTAAAGATTTTGGAGTTTCTATGATTAAGATGGGTAATTTGAAGCCTCTTACTGGGAAAAAGGGTGAGATTAGGTGTAATTGCAGAAAAGTCAATCAGAACTGA
Protein sequence

>Msa0735250

MGASLLRLHFHDCFVSGCDGSVLLDDTPTFIGEKTAFPNNNSIRGFEVVDQIKAAVTKACKRDVVSCADILAIAARDSVAIMGGNQYWYQVLLGRRDSIFASRDAANTNLPPPFFNFSQLITNFKNRIYNETNIDTNFAANLQKTCPRIGGDCNLAPFDSTPNKVDTSYYKALLYKQGLLHSDQELFKGDGSQSDRLVQLYSKNSYAFAKDFGVSMIKMGNLKPLTGKKGEIRCNCRKVNQN*