AlfalfaGEDB | Msa0736450

M. sativa cultivar zhongmu-4 / Msa0736450

KEGG: -
GO: GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0019219 GO:0019222 GO:0022411 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0090601 GO:0090602 GO:0090603 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736450	XP_003616168.1	91.091	651	51	1	1	651	1	644	0.0	1242

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736450	sp\|A4VCM0\|NAC45_ARATH	38.977	567	233	22	1	557	1	464	1.54e-107	336

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736450	G7KFT9	91.091	651	51	1	1	651	1	644	0.0	1242

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TFs/TRs:

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Gene ID	Type	Classification
Msa0736450	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0736450	Msa0855730	0.871334	7.397494e-67	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736450	MtrunA17_Chr5g0433781	91.091	651	51	1	1	651	1	644	0.0	1242
Msa0736450	MtrunA17_Chr3g0109821	63.596	673	216	19	1	650	1	667	0.0	803
Msa0736450	MtrunA17_Chr6g0481091	74.375	160	41	0	1	160	1	160	9.80e-86	268
Msa0736450	MtrunA17_Chr2g0309891	74.545	165	41	1	1	164	1	165	1.76e-85	271
Msa0736450	MtrunA17_Chr5g0400461	64.417	163	48	2	1	156	1	160	1.38e-67	224
Msa0736450	MtrunA17_Chr4g0054791	61.635	159	58	1	1	156	1	159	1.86e-64	215
Msa0736450	MtrunA17_Chr1g0201021	61.728	162	61	1	8	169	21	181	1.46e-60	212
Msa0736450	MtrunA17_Chr2g0318081	56.329	158	67	1	1	158	11	166	5.98e-60	204
Msa0736450	MtrunA17_Chr8g0372121	48.352	182	89	3	1	180	1	179	1.11e-58	199
Msa0736450	MtrunA17_Chr8g0359921	57.500	160	64	2	1	158	9	166	1.88e-58	199

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736450	AT1G65910.1	44.702	604	255	21	1	580	1	549	1.21e-148	445
Msa0736450	AT3G03200.1	38.977	567	233	22	1	557	1	464	1.57e-108	336
Msa0736450	AT5G17260.1	40.180	555	216	20	1	545	1	449	3.48e-108	335
Msa0736450	AT3G17730.1	75.000	160	40	0	1	160	1	160	3.75e-87	273
Msa0736450	AT1G54330.1	74.699	166	38	2	1	166	1	162	7.52e-82	261
Msa0736450	AT4G17980.1	63.750	160	54	2	1	156	1	160	4.38e-69	226
Msa0736450	AT4G17980.2	63.750	160	54	2	1	156	1	160	7.02e-69	226
Msa0736450	AT5G46590.1	60.248	161	62	2	1	159	1	161	3.80e-66	219
Msa0736450	AT3G10500.1	47.521	242	115	3	4	234	7	247	9.88e-63	218
Msa0736450	AT5G04410.1	59.639	166	66	1	4	169	7	171	4.74e-62	216

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Find 162 sgRNAs with CRISPR-Local

Find 330 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCCTAGTTTACTATCG+AGG	0.634321	5_1:-52911160	Msa0736450:CDS
AAACCTGGAGGCAATGAAAC+AGG	0.435510	5_1:+52912901	None:intergenic
AAAGGTTAGTCATAGAATGT+TGG	0.457270	5_1:-52908377	Msa0736450:CDS
AAAGTAAAATCATCTTCATA+AGG	0.480913	5_1:+52909365	None:intergenic
AAATTACCTGGCAAGTCCCA+CGG	0.604416	5_1:+52912766	None:intergenic
AAATTATTTGATGCAAATGA+AGG	0.433232	5_1:-52910474	Msa0736450:CDS
AACACAAGATCTATAGAGAT+AGG	0.493319	5_1:-52909266	Msa0736450:CDS
AACCAAATCTGGGTATTGGA+AGG	0.600307	5_1:-52911236	Msa0736450:CDS
AACTTGGTGAAATAATGTCA+TGG	0.614916	5_1:+52908336	None:intergenic
AAGATGATGATAATGGAGAA+TGG	0.544768	5_1:-52908235	Msa0736450:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATGGCCATAAGATTGAAT+TGG	-	chr5_1:52907984-52908003	Msa0736450:CDS	30.0%
TGATCTCCAATTCAATCTTA+TGG	+	chr5_1:52907993-52908012	None:intergenic	30.0%
GCTATAGTTCATATCATAGA+AGG	-	chr5_1:52908512-52908531	Msa0736450:CDS	30.0%
CTATACTTGACCAAAAAAGT+AGG	+	chr5_1:52908942-52908961	None:intergenic	30.0%
TATATCCTAACTAATTTGGC+GGG	-	chr5_1:52909201-52909220	Msa0736450:CDS	30.0%
ATTATTACCTGGGAATGATA+TGG	-	chr5_1:52909488-52909507	Msa0736450:CDS	30.0%
AAGTGAGAAAAAGAAAGAAG+GGG	+	chr5_1:52909765-52909784	None:intergenic	30.0%
TGAAAAATAATCACAACCTC+AGG	-	chr5_1:52910148-52910167	Msa0736450:intron	30.0%
GGACAATATGGTAATATTAC+AGG	-	chr5_1:52910230-52910249	Msa0736450:intron	30.0%
GTAGTTGGAAGACTAAATAA+TGG	+	chr5_1:52910455-52910474	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	52907895	52912935	52907895	ID=Msa0736450;Name=Msa0736450
chr5_1	mRNA	52907895	52912935	52907895	ID=Msa0736450-mRNA-1;Parent=Msa0736450;Name=Msa0736450-mRNA-1;_AED=0.03;_eAED=0.03;_QI=6\|1\|0.83\|1\|1\|1\|6\|0\|651
chr5_1	exon	52907895	52908559	52907895	ID=Msa0736450-mRNA-1:exon:14868;Parent=Msa0736450-mRNA-1
chr5_1	exon	52908955	52909030	52908955	ID=Msa0736450-mRNA-1:exon:14867;Parent=Msa0736450-mRNA-1
chr5_1	exon	52909181	52909609	52909181	ID=Msa0736450-mRNA-1:exon:14866;Parent=Msa0736450-mRNA-1
chr5_1	exon	52910310	52910660	52910310	ID=Msa0736450-mRNA-1:exon:14865;Parent=Msa0736450-mRNA-1
chr5_1	exon	52911072	52911349	52911072	ID=Msa0736450-mRNA-1:exon:14864;Parent=Msa0736450-mRNA-1
chr5_1	exon	52912773	52912935	52912773	ID=Msa0736450-mRNA-1:exon:14863;Parent=Msa0736450-mRNA-1
chr5_1	five_prime_UTR	52912930	52912935	52912930	ID=Msa0736450-mRNA-1:five_prime_utr;Parent=Msa0736450-mRNA-1
chr5_1	CDS	52912773	52912929	52912773	ID=Msa0736450-mRNA-1:cds;Parent=Msa0736450-mRNA-1
chr5_1	CDS	52911072	52911349	52911072	ID=Msa0736450-mRNA-1:cds;Parent=Msa0736450-mRNA-1
chr5_1	CDS	52910310	52910660	52910310	ID=Msa0736450-mRNA-1:cds;Parent=Msa0736450-mRNA-1
chr5_1	CDS	52909181	52909609	52909181	ID=Msa0736450-mRNA-1:cds;Parent=Msa0736450-mRNA-1
chr5_1	CDS	52908955	52909030	52908955	ID=Msa0736450-mRNA-1:cds;Parent=Msa0736450-mRNA-1
chr5_1	CDS	52907895	52908559	52907895	ID=Msa0736450-mRNA-1:cds;Parent=Msa0736450-mRNA-1

Gene Sequence

>Msa0736450

ATGGCACCTGTTTCATTGCCTCCAGGTTTCAGGTTCCACCCTACGGATGAAGAACTTGTTGCTTATTATCTAAATAGGAAGATTAATGGCCATAAGATTGAATTGGAGATCATAGCTGAAGTTGATCTCTATAAGTGTGAACCGTGGGACTTGCCAGGGAAATCATTATTACCTGGGAATGATATGGAGTGGTATTTCTTTAGCCCTCGTGATAGGAAGTACCCGAATGGATCTAGAACGAATCGTGCAACCAAATCTGGGTATTGGAAGGCAACCGGGAAGGATCGAAAAGTGAATTCTCAAGCTCGAGCAGTAGGGGTGAAGAAAACCCTAGTTTACTATCGAGGAAGAGCACCCCATGGCTCTCGTACAAATTGGGTTATGCATGAATATCGTCTTGATGAGAGGGAATGTGAAACCACTCCTAGCTTGCAGGATGCATATGCTCTTTGCCGTGTGATCAAGAAGAGTACAGTGATTGTGCCAAAAGTTGGAGGACAATATGGTAATATTACAGGCCATGCAAACCAGATAACAAGTGACCAATCAACAAACGTTGAGCTATACTCTGAAAGAAGGGGTGAAAATTTAGCTAGTTCAAATTATTTGATGCAAATGAAGGCTTGCTCACCTCACAATATGGTACATGAGACTTCTCTCAATATCAATGGTACGACAAGGGATCATGGAAATTGGGCGCAATTCTCATCTGAAGACCCATTATTTAGTCTTCCAACTACTTCTTCATTTACTAATTATGGAGCCATAACATACCCTCCATCCAAGGTGGATATTGCACTAGAGTGTGCAAGGATGCAACATAGGTTTACTATGCCTCCATTGGAGGTGGAGGAATTTCCTCAAGTTGGAATACCTCATGTGACACAACAAGGAAGCAGAATTGCAAATGATATCTTGCAGGGAATTCTTTCACTTAATCATGCTTCTCAGGGATTGATGAATCAGTCCAGTTATTTACAAGCCTTGGATGACAATGAAAATTATGCTCCTTATGAAGATGATTTTACTTTCATGGTTGACACTAACTACAATCACATGAATGATATGAGCTCCATGGGATATGTTGACAAAACTTGGGAAGATTTGAACACAAGATCTATAGAGATAGGTGATCTAGAGGATCAAGAGTCAAAGGCCAAACGGATGGTAGAGAATTTGAGATGGATTGGAATGCCAAGAGAAGATTTTGAAAAGATGGAAGAACAAAATGTTGTCCCAATCGAGGAGATTTCATGCTCCCAAACATCAAGGGAACACAATGGCATGCAAGAATACGAGCGGCACAATAAGAACCTTGAACAAAATGACAATGATGTCAATGAATTCTCATGGGGATTCATCAATGATGGTGACCCCAACGAGAATTTTATAGAAGATGGTAGCATGGATCATTACTCAAATTCTCCAAGCTTTGAGATCACTGAGGAAATAAAGGTTAGTCATAGAATGTTGGTTTCAACTCAACAAGTAGCCATGACATTATTTCACCAAGTTGTTCCTTCACAAACACTTCAAATTCAACTCAATCCAATGATGCCAAACAATAAACACTCTATAGAGAACGTAGATGCAAAGATGATGATAATGGAGAATGGAGGATCTTTCTTCATGAAGTTCAAGGCCTATGTGATGGGGAAATGGAACCTATTCAACCCATCAAAGAAAATAGCAAGTTTGGTTGTATTTGTATTTGCACTTCTTTTGATGCATCGTGTTTATTTGGAGGAAGAAGTAGAAATCTGCAAACCAAAACCAAAAAAGATGAAAGAGAAAAGAATGAGAGAATCACATGAGAGAGTCAAATGGAATGAGTCAAAAGTGGTTTGGTTTGTTGGTGTAAAATGTGAGAAAGGATTAAGTGTGGTCTTGAAGGAAATAGGCATTTTCTTCACCATATTTTTGGCGCTTTGTACCACATGGGCTAGCCATGGCTAA

Protein sequence

>Msa0736450

MAPVSLPPGFRFHPTDEELVAYYLNRKINGHKIELEIIAEVDLYKCEPWDLPGKSLLPGNDMEWYFFSPRDRKYPNGSRTNRATKSGYWKATGKDRKVNSQARAVGVKKTLVYYRGRAPHGSRTNWVMHEYRLDERECETTPSLQDAYALCRVIKKSTVIVPKVGGQYGNITGHANQITSDQSTNVELYSERRGENLASSNYLMQMKACSPHNMVHETSLNINGTTRDHGNWAQFSSEDPLFSLPTTSSFTNYGAITYPPSKVDIALECARMQHRFTMPPLEVEEFPQVGIPHVTQQGSRIANDILQGILSLNHASQGLMNQSSYLQALDDNENYAPYEDDFTFMVDTNYNHMNDMSSMGYVDKTWEDLNTRSIEIGDLEDQESKAKRMVENLRWIGMPREDFEKMEEQNVVPIEEISCSQTSREHNGMQEYERHNKNLEQNDNDVNEFSWGFINDGDPNENFIEDGSMDHYSNSPSFEITEEIKVSHRMLVSTQQVAMTLFHQVVPSQTLQIQLNPMMPNNKHSIENVDAKMMIMENGGSFFMKFKAYVMGKWNLFNPSKKIASLVVFVFALLLMHRVYLEEEVEICKPKPKKMKEKRMRESHERVKWNESKVVWFVGVKCEKGLSVVLKEIGIFFTIFLALCTTWASHG*