AlfalfaGEDB | Msa0746260

M. sativa cultivar zhongmu-4 / Msa0746260

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746260	XP_003603231.1	56.774	155	53	3	1	145	165	315	7.25e-41	156

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746260	sp\|O64842\|PBL12_ARATH	75.000	88	20	1	1	88	171	256	2.67e-35	134

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746260	G7J941	56.774	155	53	3	1	145	165	315	3.46e-41	156

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0331080	Msa0746260	0.842371	2.693005e-58	-8.615850e-47
Msa0746260	Msa1301450	0.820160	7.944522e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746260	MtrunA17_Chr3g0136421	56.774	155	53	3	1	145	165	315	6.67e-45	156
Msa0746260	MtrunA17_Chr1g0150741	55.102	147	58	3	1	144	165	306	7.66e-42	148
Msa0746260	MtrunA17_Chr2g0300711	50.000	156	61	4	1	145	162	311	1.04e-36	137
Msa0746260	MtrunA17_Chr4g0070411	52.555	137	59	3	1	137	168	298	9.78e-36	134
Msa0746260	MtrunA17_Chr7g0245571	71.605	81	23	0	1	81	174	254	2.00e-32	124
Msa0746260	MtrunA17_Chr5g0416011	75.610	82	18	2	1	81	222	302	3.51e-32	124
Msa0746260	MtrunA17_Chr8g0362661	74.390	82	19	2	1	81	220	300	2.19e-31	122
Msa0746260	MtrunA17_Chr7g0216551	69.512	82	24	1	1	81	209	290	4.39e-31	120
Msa0746260	MtrunA17_Chr4g0064201	70.370	81	23	1	1	81	197	276	1.53e-30	119
Msa0746260	MtrunA17_Chr5g0408941	67.045	88	26	2	1	88	175	259	1.91e-30	118

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746260	AT2G26290.1	75.000	88	20	1	1	88	171	256	2.71e-36	134
Msa0746260	AT2G05940.1	77.778	81	18	0	1	81	170	250	8.39e-36	134
Msa0746260	AT5G01020.1	72.840	81	22	0	1	81	152	232	7.50e-33	125
Msa0746260	AT5G35580.1	70.370	81	24	0	1	81	159	239	3.44e-32	124
Msa0746260	AT5G35580.2	70.370	81	24	0	1	81	171	251	3.71e-32	124
Msa0746260	AT1G61590.1	65.909	88	28	1	1	88	182	267	1.17e-30	119
Msa0746260	AT5G56460.1	67.901	81	26	0	1	81	162	242	5.03e-29	114
Msa0746260	AT2G28930.1	47.887	142	60	6	1	137	166	298	5.35e-29	115
Msa0746260	AT2G28930.3	66.265	83	26	1	1	81	158	240	5.71e-29	114
Msa0746260	AT2G28930.2	47.887	142	60	6	1	137	155	287	5.91e-29	114

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Find 87 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTGGAGGAAAAGATA+TGG	0.381962	5_1:+68414311	None:intergenic
AAATGTGCACCACAATCTGC+AGG	0.517804	5_1:-68412902	Msa0746260:CDS
AACAGAGTCATACCAGTGGT+TGG	0.615367	5_1:+68416304	None:intergenic
AACCTGAGAAATAGAGCAAC+CGG	0.455042	5_1:+68416994	None:intergenic
AACCTTGGATTAGCTGAAGA+TGG	0.530476	5_1:-68415078	Msa0746260:CDS
AAGAAGGAAAAGAAGCCATG+AGG	0.668105	5_1:-68416332	Msa0746260:five_prime_UTR
AAGCTTCCTTTCATAATGGA+AGG	0.546475	5_1:-68412407	Msa0746260:CDS
AAGGATTTGATGTCTAGATT+TGG	0.358983	5_1:-68416442	Msa0746260:five_prime_UTR
AATGGACCTTCCATTATGAA+AGG	0.578687	5_1:+68412401	None:intergenic
AATGTGCACCACAATCTGCA+GGG	0.681242	5_1:-68412901	Msa0746260:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCAAGGTACATAAATAAAAG+AGG	+	chr5_1:68411874-68411893	None:intergenic	30.0%
AACACTTGGAGTCAATTTAA+TGG	+	chr5_1:68412269-68412288	None:intergenic	30.0%
AAGGATTTGATGTCTAGATT+TGG	-	chr5_1:68412304-68412323	Msa0746260:intron	30.0%
GAAAAGGTAATATGATCTCA+AGG	-	chr5_1:68412474-68412493	Msa0746260:intron	30.0%
CAAAATTTCACTCAAGCTTA+TGG	-	chr5_1:68412741-68412760	Msa0746260:intron	30.0%
AGGACTTAAAAAATCTCACA+AGG	-	chr5_1:68412849-68412868	Msa0746260:intron	30.0%
TCCAAATATATTACTGGACT+CGG	-	chr5_1:68413541-68413560	Msa0746260:intron	30.0%
GTTGTGTAATCCTGAATTTA+GGG	+	chr5_1:68413638-68413657	None:intergenic	30.0%
AGTTGTGTAATCCTGAATTT+AGG	+	chr5_1:68413639-68413658	None:intergenic	30.0%
ACAACTAAACTATCAAACCT+TGG	-	chr5_1:68413653-68413672	Msa0746260:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	68411736	68417032	68411736	ID=Msa0746260;Name=Msa0746260
chr5_1	mRNA	68411736	68417032	68411736	ID=Msa0746260-mRNA-1;Parent=Msa0746260;Name=Msa0746260-mRNA-1;_AED=0.30;_eAED=0.30;_QI=516\|0.57\|0.6\|1\|0.71\|0.6\|15\|151\|293
chr5_1	exon	68411736	68411937	68411736	ID=Msa0746260-mRNA-1:exon:20424;Parent=Msa0746260-mRNA-1
chr5_1	exon	68412353	68412444	68412353	ID=Msa0746260-mRNA-1:exon:20423;Parent=Msa0746260-mRNA-1
chr5_1	exon	68412887	68412941	68412887	ID=Msa0746260-mRNA-1:exon:20422;Parent=Msa0746260-mRNA-1
chr5_1	exon	68413026	68413095	68413026	ID=Msa0746260-mRNA-1:exon:20421;Parent=Msa0746260-mRNA-1
chr5_1	exon	68413586	68413801	68413586	ID=Msa0746260-mRNA-1:exon:20420;Parent=Msa0746260-mRNA-1
chr5_1	exon	68414216	68414342	68414216	ID=Msa0746260-mRNA-1:exon:20419;Parent=Msa0746260-mRNA-1
chr5_1	exon	68414586	68414616	68414586	ID=Msa0746260-mRNA-1:exon:20418;Parent=Msa0746260-mRNA-1
chr5_1	exon	68415056	68415121	68415056	ID=Msa0746260-mRNA-1:exon:20417;Parent=Msa0746260-mRNA-1
chr5_1	exon	68415206	68415342	68415206	ID=Msa0746260-mRNA-1:exon:20416;Parent=Msa0746260-mRNA-1
chr5_1	exon	68415445	68415577	68415445	ID=Msa0746260-mRNA-1:exon:20415;Parent=Msa0746260-mRNA-1
chr5_1	exon	68415898	68415936	68415898	ID=Msa0746260-mRNA-1:exon:20414;Parent=Msa0746260-mRNA-1
chr5_1	exon	68415965	68416061	68415965	ID=Msa0746260-mRNA-1:exon:20413;Parent=Msa0746260-mRNA-1
chr5_1	exon	68416289	68416461	68416289	ID=Msa0746260-mRNA-1:exon:20412;Parent=Msa0746260-mRNA-1
chr5_1	exon	68416554	68416628	68416554	ID=Msa0746260-mRNA-1:exon:20411;Parent=Msa0746260-mRNA-1
chr5_1	exon	68416997	68417032	68416997	ID=Msa0746260-mRNA-1:exon:20410;Parent=Msa0746260-mRNA-1
chr5_1	five_prime_UTR	68416997	68417032	68416997	ID=Msa0746260-mRNA-1:five_prime_utr;Parent=Msa0746260-mRNA-1
chr5_1	five_prime_UTR	68416554	68416628	68416554	ID=Msa0746260-mRNA-1:five_prime_utr;Parent=Msa0746260-mRNA-1
chr5_1	five_prime_UTR	68416289	68416461	68416289	ID=Msa0746260-mRNA-1:five_prime_utr;Parent=Msa0746260-mRNA-1
chr5_1	five_prime_UTR	68415965	68416061	68415965	ID=Msa0746260-mRNA-1:five_prime_utr;Parent=Msa0746260-mRNA-1
chr5_1	five_prime_UTR	68415898	68415936	68415898	ID=Msa0746260-mRNA-1:five_prime_utr;Parent=Msa0746260-mRNA-1
chr5_1	five_prime_UTR	68415482	68415577	68415482	ID=Msa0746260-mRNA-1:five_prime_utr;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68415445	68415481	68415445	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68415206	68415342	68415206	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68415056	68415121	68415056	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68414586	68414616	68414586	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68414216	68414342	68414216	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68413586	68413801	68413586	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68413026	68413095	68413026	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68412887	68412941	68412887	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68412353	68412444	68412353	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	CDS	68411887	68411937	68411887	ID=Msa0746260-mRNA-1:cds;Parent=Msa0746260-mRNA-1
chr5_1	three_prime_UTR	68411736	68411886	68411736	ID=Msa0746260-mRNA-1:three_prime_utr;Parent=Msa0746260-mRNA-1

Gene Sequence

>Msa0746260

ATGACAAGAGGGGGCTTGGAGAATCAATTGTTCAGAAGATCCTCTGCTACATTACCATGGTCAACTAGAATGAAAATTGCATTAGGTGCAGCTAAGGGACTAGCTTTCCTTCATGAAGCAGATAAACTTGTAATATATAGGGATTTCAAAACTCCAAATATATTACTGGACTCGGATTACACAACTAAACTATCAAACCTTGGATTAGCTGAAGATGGTCCTGAAGGAGAAGAAACACATGTCATCTTTCTACCAAGAGTGATGTGTATAGAGTTACAAACCATATCTTTTCCTCCAAATTTTGATCTTATTGGCAGCGCTGGTAATCATTCGGCTGCAGTGACTTATATCATAATTAGCATTGGCGGTTGCATGATCCTCAGTTGTAGTGCTGATAGGTTTAGGCCACCAATTCTTATCGATGTGAGCAAGATAGACATGTCAACTACTGTCCTTGGCTTCAAAATTTCCATGCCAGTCATGATTGCTCCAACTGCTATGCAGAAAATGGCTCATCCGGAGGGTATCATTGCTTTTTCTTTAAATTCACTTGAATTTCTGAAATGCAAAATTAAGTTGTTTTGTTTTGTGCAATATTTTGTTGGAAGGAAAAAACAGCCTGGTTTTAAGCAAATGAAGATTATAAGGCAGGAGAGGCATACAGTTTGCCTCATTGAGTTTGAAGATGTGAATAGTGCTACAAATGTGCACCACAATCTGCAGGGTGCTGTTATCCCAAGGTTGCAACATGACAAAGCTTCCTTTCATAATGGAAGGTCCATTTCTGCTACTGCCATATTGTTGCAGGTGATTTTTGTTGGCACGGGTAAGGATCCTTACTCACTTGATATAGGAAGATTTTTCACAGGATTTGGAATTTGA

Protein sequence

>Msa0746260

MTRGGLENQLFRRSSATLPWSTRMKIALGAAKGLAFLHEADKLVIYRDFKTPNILLDSDYTTKLSNLGLAEDGPEGEETHVIFLPRVMCIELQTISFPPNFDLIGSAGNHSAAVTYIIISIGGCMILSCSADRFRPPILIDVSKIDMSTTVLGFKISMPVMIAPTAMQKMAHPEGIIAFSLNSLEFLKCKIKLFCFVQYFVGRKKQPGFKQMKIIRQERHTVCLIEFEDVNSATNVHHNLQGAVIPRLQHDKASFHNGRSISATAILLQVIFVGTGKDPYSLDIGRFFTGFGI*