AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0748270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748270 MtrunA17_Chr7g0255841 100.000 92 0 0 1 92 1 92 7.13e-65 190
Msa0748270 MtrunA17_Chr3g0081631 72.222 90 24 1 4 92 2 91 1.26e-44 139
Msa0748270 MtrunA17_Chr6g0449111 72.059 68 19 0 7 74 5 72 6.26e-35 114
Msa0748270 MtrunA17_Chr3g0144631 68.354 79 21 1 1 75 1 79 5.32e-34 112
Msa0748270 MtrunA17_Chr1g0155181 59.302 86 29 1 6 85 1 86 6.14e-33 109
Msa0748270 MtrunA17_Chr3g0141601 57.143 91 35 2 6 92 1 91 3.14e-32 107
Msa0748270 MtrunA17_Chr5g0404101 63.014 73 27 0 4 76 11 83 1.24e-30 103
Msa0748270 MtrunA17_Chr8g0373131 56.716 67 28 1 9 75 2 67 7.81e-25 88.6
Msa0748270 MtrunA17_Chr8g0373111 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
Msa0748270 MtrunA17_Chr8g0373121 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
Msa0748270 MtrunA17_Chr3g0141641 45.055 91 41 2 11 92 2 92 3.32e-24 87.4
Msa0748270 MtrunA17_Chr5g0404301 60.317 63 24 1 10 72 2 63 9.33e-24 85.5
Msa0748270 MtrunA17_Chr8g0373101 56.452 62 26 1 11 72 4 64 6.78e-23 83.6
Msa0748270 MtrunA17_Chr5g0436501 54.930 71 32 0 12 82 27 97 1.30e-20 82.8
Msa0748270 MtrunA17_Chr8g0364741 50.667 75 37 0 6 80 4 78 2.62e-20 78.6
Msa0748270 MtrunA17_Chr1g0162081 50.000 78 39 0 1 78 1 78 1.38e-19 80.5
Msa0748270 MtrunA17_Chr8g0365011 54.167 72 33 0 9 80 5 76 2.84e-19 79.3
Msa0748270 MtrunA17_Chr3g0104071 50.685 73 36 0 2 74 33 105 9.65e-19 78.2
Msa0748270 MtrunA17_Chr5g0429521 47.826 69 36 0 11 79 28 96 5.02e-18 76.3
Msa0748270 MtrunA17_Chr7g0241761 52.174 69 33 0 12 80 35 103 5.19e-18 76.3
Msa0748270 MtrunA17_Chr1g0194071 57.627 59 24 1 11 68 10 68 6.75e-16 66.6
Msa0748270 MtrunA17_Chr1g0210471 47.619 63 33 0 12 74 24 86 7.82e-15 67.0
Msa0748270 MtrunA17_Chr7g0270711 55.000 60 26 1 11 69 10 69 3.19e-14 62.0
Msa0748270 MtrunA17_Chr4g0062741 45.205 73 36 2 6 74 1 73 4.56e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748270 AT2G21650.1 64.773 88 24 1 12 92 14 101 4.16e-38 123
Msa0748270 AT4G39250.1 64.368 87 25 1 12 92 14 100 1.32e-37 122
Msa0748270 AT1G75250.2 55.670 97 37 1 1 91 1 97 5.60e-36 117
Msa0748270 AT1G75250.1 69.333 75 23 0 1 75 1 75 1.47e-35 117
Msa0748270 AT1G19510.1 59.783 92 37 0 1 92 1 92 2.64e-29 100
Msa0748270 AT2G18328.1 62.295 61 23 0 15 75 14 74 1.84e-24 88.2
Msa0748270 AT5G05790.2 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
Msa0748270 AT5G05790.1 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
Msa0748270 AT4G36570.1 75.000 44 11 0 12 55 12 55 4.92e-20 76.3
Msa0748270 AT3G11280.2 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
Msa0748270 AT3G11280.1 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
Msa0748270 AT2G38090.1 52.174 69 33 0 12 80 29 97 2.27e-18 77.4
Msa0748270 AT5G58900.1 50.000 74 34 1 4 74 23 96 3.03e-17 74.3
Msa0748270 AT5G08520.1 52.381 63 30 0 12 74 12 74 5.44e-16 70.9
Msa0748270 AT1G49010.1 46.479 71 35 1 7 74 3 73 1.43e-14 67.4
Msa0748270 AT5G23650.1 46.377 69 37 0 6 74 7 75 2.01e-14 67.0
Msa0748270 AT5G04760.1 41.791 67 38 1 8 74 2 67 4.55e-13 62.4
Msa0748270 AT5G01200.1 42.424 66 37 1 10 74 28 93 2.05e-11 58.2

Find 33 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCTAGTATCCTAATTAA+TGG 0.186484 5_2:+1427632 Msa0748270:three_prime_UTR
AACAATTAATAAGCTAGTTT+TGG 0.255773 5_2:-1427082 None:intergenic
ACCCCTCAATACCATTAATT+AGG 0.339309 5_2:-1427643 None:intergenic
TACTTTCTTGTTGTTGAAGC+AGG 0.354538 5_2:+1427773 Msa0748270:three_prime_UTR
TCTAGTTCAATGTCAGCTTC+TGG 0.381239 5_2:+1427138 Msa0748270:CDS
GATATTAAACATATTGAGTC+AGG 0.394581 5_2:+1427309 Msa0748270:CDS
TTGTTGTTGATTGCACTTGT+TGG 0.397526 5_2:-1427595 None:intergenic
TAATGTTTCTTAACTTCCTC+AGG 0.405406 5_2:-1427270 None:intergenic
GCCATTTCTTAATTAATTCA+AGG 0.418850 5_2:-1427114 None:intergenic
ACCTTGAATTAATTAAGAAA+TGG 0.425549 5_2:+1427113 Msa0748270:exon
ATCCTAATTAATGGTATTGA+GGG 0.440615 5_2:+1427641 Msa0748270:three_prime_UTR
CAGTGTCTCATGAGGAGAAA+AGG 0.467404 5_2:+1427364 Msa0748270:CDS
TATCCTAATTAATGGTATTG+AGG 0.477146 5_2:+1427640 Msa0748270:three_prime_UTR
CAATGTCAGCTTCTGGCTCA+TGG 0.480309 5_2:+1427145 Msa0748270:CDS
TTCTGGCTCATGGAGTGTTA+AGG 0.488975 5_2:+1427155 Msa0748270:CDS
TTGCACTTGTTGGTCAATGA+AGG 0.513934 5_2:-1427585 None:intergenic
GCTACATTGTACCAACGATC+TGG 0.520197 5_2:-1427225 None:intergenic
TAATTAATTCAAGGTATGAA+AGG 0.527947 5_2:-1427105 None:intergenic
CACTTATGAAGAGTGGTAAG+TGG 0.542822 5_2:-1427050 None:intergenic
CTTCAACAACAAGAAAGTAA+AGG 0.546066 5_2:-1427769 None:intergenic
AACAGCTAAAGCTCTTTCAA+AGG 0.549456 5_2:-1427187 None:intergenic
ACATTATGAACTTCTTGTTG+AGG 0.570098 5_2:+1427287 Msa0748270:CDS
AACAACAAGAAAGTAAAGGA+TGG 0.573425 5_2:-1427765 None:intergenic
GAAAATTTCAGTGTCTCATG+AGG 0.590467 5_2:+1427356 Msa0748270:CDS
TACAATGTAGCTCATGCTGT+TGG 0.593737 5_2:+1427237 Msa0748270:CDS
TGAGAATCACTTATGAAGAG+TGG 0.595892 5_2:-1427057 None:intergenic
AATGTAGCTCATGCTGTTGG+TGG 0.602491 5_2:+1427240 Msa0748270:CDS
ACAAAGACACTCCAGATCGT+TGG 0.612332 5_2:+1427214 Msa0748270:CDS
CAACTTTATTAAGAACGAGG+AGG 0.657197 5_2:-1427820 None:intergenic
ATATTAAACATATTGAGTCA+GGG 0.667795 5_2:+1427310 Msa0748270:CDS
TCCTAATTAATGGTATTGAG+GGG 0.676186 5_2:+1427642 Msa0748270:three_prime_UTR
TTACAACTTTATTAAGAACG+AGG 0.684701 5_2:-1427823 None:intergenic
TGTTGGTGGCAAAACTCCTG+AGG 0.702099 5_2:+1427254 Msa0748270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAATTAATAAGCTAGTTT+TGG - chr5_2:1427085-1427104 None:intergenic 20.0%
!! TAATTAATTCAAGGTATGAA+AGG - chr5_2:1427108-1427127 None:intergenic 20.0%
!! ACCTTGAATTAATTAAGAAA+TGG + chr5_2:1427113-1427132 Msa0748270:exon 20.0%
!! ATATTAAACATATTGAGTCA+GGG + chr5_2:1427310-1427329 Msa0748270:CDS 20.0%
!!! ATTTTCTTGTAATTTGGAAA+TGG - chr5_2:1427342-1427361 None:intergenic 20.0%
!!! ACTGAAATTTTCTTGTAATT+TGG - chr5_2:1427348-1427367 None:intergenic 20.0%
!! TTTAATTATTTAACAAGCGT+AGG - chr5_2:1427435-1427454 None:intergenic 20.0%
!!! TACTCTTTACTTTCTTTTAT+AGG + chr5_2:1427548-1427567 Msa0748270:intron 20.0%
!! TATTCTAGTATCCTAATTAA+TGG + chr5_2:1427632-1427651 Msa0748270:three_prime_UTR 20.0%
!!! ATTTTATCCAAAAAGAGTAA+AGG - chr5_2:1427744-1427763 None:intergenic 20.0%
!!! AACAATTAATAAGCTAGTTT+TGG - chr5_2:1427085-1427104 None:intergenic 20.0%
!! TAATTAATTCAAGGTATGAA+AGG - chr5_2:1427108-1427127 None:intergenic 20.0%
!! ACCTTGAATTAATTAAGAAA+TGG + chr5_2:1427113-1427132 Msa0748270:exon 20.0%
!! ATATTAAACATATTGAGTCA+GGG + chr5_2:1427310-1427329 Msa0748270:CDS 20.0%
!!! ATTTTCTTGTAATTTGGAAA+TGG - chr5_2:1427342-1427361 None:intergenic 20.0%
!!! ACTGAAATTTTCTTGTAATT+TGG - chr5_2:1427348-1427367 None:intergenic 20.0%
!! TTTAATTATTTAACAAGCGT+AGG - chr5_2:1427435-1427454 None:intergenic 20.0%
!!! TACTCTTTACTTTCTTTTAT+AGG + chr5_2:1427548-1427567 Msa0748270:intron 20.0%
!! TATTCTAGTATCCTAATTAA+TGG + chr5_2:1427632-1427651 Msa0748270:three_prime_UTR 20.0%
!!! ATTTTATCCAAAAAGAGTAA+AGG - chr5_2:1427744-1427763 None:intergenic 20.0%
! GCCATTTCTTAATTAATTCA+AGG - chr5_2:1427117-1427136 None:intergenic 25.0%
! GATATTAAACATATTGAGTC+AGG + chr5_2:1427309-1427328 Msa0748270:CDS 25.0%
!! TTACTTTCTTTTATAGGATG+AGG + chr5_2:1427554-1427573 Msa0748270:intron 25.0%
! TATCCTAATTAATGGTATTG+AGG + chr5_2:1427640-1427659 Msa0748270:three_prime_UTR 25.0%
!! ATCCTAATTAATGGTATTGA+GGG + chr5_2:1427641-1427660 Msa0748270:three_prime_UTR 25.0%
!! ATTCTATCCTTTACTCTTTT+TGG + chr5_2:1427734-1427753 Msa0748270:three_prime_UTR 25.0%
! TTACAACTTTATTAAGAACG+AGG - chr5_2:1427826-1427845 None:intergenic 25.0%
! GCCATTTCTTAATTAATTCA+AGG - chr5_2:1427117-1427136 None:intergenic 25.0%
! GATATTAAACATATTGAGTC+AGG + chr5_2:1427309-1427328 Msa0748270:CDS 25.0%
!! TTACTTTCTTTTATAGGATG+AGG + chr5_2:1427554-1427573 Msa0748270:intron 25.0%
! TATCCTAATTAATGGTATTG+AGG + chr5_2:1427640-1427659 Msa0748270:three_prime_UTR 25.0%
!! ATCCTAATTAATGGTATTGA+GGG + chr5_2:1427641-1427660 Msa0748270:three_prime_UTR 25.0%
!! ATTCTATCCTTTACTCTTTT+TGG + chr5_2:1427734-1427753 Msa0748270:three_prime_UTR 25.0%
! TTACAACTTTATTAAGAACG+AGG - chr5_2:1427826-1427845 None:intergenic 25.0%
TAATGTTTCTTAACTTCCTC+AGG - chr5_2:1427273-1427292 None:intergenic 30.0%
ACATTATGAACTTCTTGTTG+AGG + chr5_2:1427287-1427306 Msa0748270:CDS 30.0%
! TCCTAATTAATGGTATTGAG+GGG + chr5_2:1427642-1427661 Msa0748270:three_prime_UTR 30.0%
AACAACAAGAAAGTAAAGGA+TGG - chr5_2:1427768-1427787 None:intergenic 30.0%
CTTCAACAACAAGAAAGTAA+AGG - chr5_2:1427772-1427791 None:intergenic 30.0%
TAATGTTTCTTAACTTCCTC+AGG - chr5_2:1427273-1427292 None:intergenic 30.0%
ACATTATGAACTTCTTGTTG+AGG + chr5_2:1427287-1427306 Msa0748270:CDS 30.0%
! TCCTAATTAATGGTATTGAG+GGG + chr5_2:1427642-1427661 Msa0748270:three_prime_UTR 30.0%
AACAACAAGAAAGTAAAGGA+TGG - chr5_2:1427768-1427787 None:intergenic 30.0%
CTTCAACAACAAGAAAGTAA+AGG - chr5_2:1427772-1427791 None:intergenic 30.0%
TGAGAATCACTTATGAAGAG+TGG - chr5_2:1427060-1427079 None:intergenic 35.0%
AACAGCTAAAGCTCTTTCAA+AGG - chr5_2:1427190-1427209 None:intergenic 35.0%
GAAAATTTCAGTGTCTCATG+AGG + chr5_2:1427356-1427375 Msa0748270:CDS 35.0%
! TTGTTGTTGATTGCACTTGT+TGG - chr5_2:1427598-1427617 None:intergenic 35.0%
ACCCCTCAATACCATTAATT+AGG - chr5_2:1427646-1427665 None:intergenic 35.0%
!! TACTTTCTTGTTGTTGAAGC+AGG + chr5_2:1427773-1427792 Msa0748270:three_prime_UTR 35.0%
CAACTTTATTAAGAACGAGG+AGG - chr5_2:1427823-1427842 None:intergenic 35.0%
TGAGAATCACTTATGAAGAG+TGG - chr5_2:1427060-1427079 None:intergenic 35.0%
AACAGCTAAAGCTCTTTCAA+AGG - chr5_2:1427190-1427209 None:intergenic 35.0%
GAAAATTTCAGTGTCTCATG+AGG + chr5_2:1427356-1427375 Msa0748270:CDS 35.0%
! TTGTTGTTGATTGCACTTGT+TGG - chr5_2:1427598-1427617 None:intergenic 35.0%
ACCCCTCAATACCATTAATT+AGG - chr5_2:1427646-1427665 None:intergenic 35.0%
!! TACTTTCTTGTTGTTGAAGC+AGG + chr5_2:1427773-1427792 Msa0748270:three_prime_UTR 35.0%
CAACTTTATTAAGAACGAGG+AGG - chr5_2:1427823-1427842 None:intergenic 35.0%
CACTTATGAAGAGTGGTAAG+TGG - chr5_2:1427053-1427072 None:intergenic 40.0%
! TCTAGTTCAATGTCAGCTTC+TGG + chr5_2:1427138-1427157 Msa0748270:CDS 40.0%
TACAATGTAGCTCATGCTGT+TGG + chr5_2:1427237-1427256 Msa0748270:CDS 40.0%
! TTGCACTTGTTGGTCAATGA+AGG - chr5_2:1427588-1427607 None:intergenic 40.0%
CACTTATGAAGAGTGGTAAG+TGG - chr5_2:1427053-1427072 None:intergenic 40.0%
! TCTAGTTCAATGTCAGCTTC+TGG + chr5_2:1427138-1427157 Msa0748270:CDS 40.0%
TACAATGTAGCTCATGCTGT+TGG + chr5_2:1427237-1427256 Msa0748270:CDS 40.0%
! TTGCACTTGTTGGTCAATGA+AGG - chr5_2:1427588-1427607 None:intergenic 40.0%
!! TTCTGGCTCATGGAGTGTTA+AGG + chr5_2:1427155-1427174 Msa0748270:CDS 45.0%
ACAAAGACACTCCAGATCGT+TGG + chr5_2:1427214-1427233 Msa0748270:CDS 45.0%
GCTACATTGTACCAACGATC+TGG - chr5_2:1427228-1427247 None:intergenic 45.0%
AATGTAGCTCATGCTGTTGG+TGG + chr5_2:1427240-1427259 Msa0748270:CDS 45.0%
CAGTGTCTCATGAGGAGAAA+AGG + chr5_2:1427364-1427383 Msa0748270:CDS 45.0%
ATGAAGAGTGGTAAGTGGAG+AGG - chr5_2:1427048-1427067 None:intergenic 45.0%
!! TTCTGGCTCATGGAGTGTTA+AGG + chr5_2:1427155-1427174 Msa0748270:CDS 45.0%
ACAAAGACACTCCAGATCGT+TGG + chr5_2:1427214-1427233 Msa0748270:CDS 45.0%
GCTACATTGTACCAACGATC+TGG - chr5_2:1427228-1427247 None:intergenic 45.0%
AATGTAGCTCATGCTGTTGG+TGG + chr5_2:1427240-1427259 Msa0748270:CDS 45.0%
CAGTGTCTCATGAGGAGAAA+AGG + chr5_2:1427364-1427383 Msa0748270:CDS 45.0%
!! CAATGTCAGCTTCTGGCTCA+TGG + chr5_2:1427145-1427164 Msa0748270:CDS 50.0%
!! TGTTGGTGGCAAAACTCCTG+AGG + chr5_2:1427254-1427273 Msa0748270:CDS 50.0%
!! CAATGTCAGCTTCTGGCTCA+TGG + chr5_2:1427145-1427164 Msa0748270:CDS 50.0%
!! TGTTGGTGGCAAAACTCCTG+AGG + chr5_2:1427254-1427273 Msa0748270:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 1427047 1427947 1427047 ID=Msa0748270;Name=Msa0748270
chr5_2 mRNA 1427047 1427947 1427047 ID=Msa0748270-mRNA-1;Parent=Msa0748270;Name=Msa0748270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=85|1|1|1|1|1|2|353|92
chr5_2 exon 1427047 1427385 1427047 ID=Msa0748270-mRNA-1:exon:372;Parent=Msa0748270-mRNA-1
chr5_2 exon 1427570 1427947 1427570 ID=Msa0748270-mRNA-1:exon:373;Parent=Msa0748270-mRNA-1
chr5_2 five_prime_UTR 1427047 1427131 1427047 ID=Msa0748270-mRNA-1:five_prime_utr;Parent=Msa0748270-mRNA-1
chr5_2 CDS 1427132 1427385 1427132 ID=Msa0748270-mRNA-1:cds;Parent=Msa0748270-mRNA-1
chr5_2 CDS 1427570 1427594 1427570 ID=Msa0748270-mRNA-1:cds;Parent=Msa0748270-mRNA-1
chr5_2 three_prime_UTR 1427595 1427947 1427595 ID=Msa0748270-mRNA-1:three_prime_utr;Parent=Msa0748270-mRNA-1
Gene Sequence

>Msa0748270

ATGGCATCTAGTTCAATGTCAGCTTCTGGCTCATGGAGTGTTAAGGAGAACAAAGCCTTTGAAAGAGCTTTAGCTGTTTATGACAAAGACACTCCAGATCGTTGGTACAATGTAGCTCATGCTGTTGGTGGCAAAACTCCTGAGGAAGTTAAGAAACATTATGAACTTCTTGTTGAGGATATTAAACATATTGAGTCAGGGAAAGTTCCATTTCCAAATTACAAGAAAATTTCAGTGTCTCATGAGGAGAAAAGGATGAGGAACATGAGCCTTCATTGA

Protein sequence

>Msa0748270

MASSSMSASGSWSVKENKAFERALAVYDKDTPDRWYNVAHAVGGKTPEEVKKHYELLVEDIKHIESGKVPFPNYKKISVSHEEKRMRNMSLH*