AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1347060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1347060 MtrunA17_Chr7g0255841 100.000 92 0 0 1 92 1 92 7.13e-65 190
Msa1347060 MtrunA17_Chr3g0081631 72.222 90 24 1 4 92 2 91 1.26e-44 139
Msa1347060 MtrunA17_Chr6g0449111 72.059 68 19 0 7 74 5 72 6.26e-35 114
Msa1347060 MtrunA17_Chr3g0144631 68.354 79 21 1 1 75 1 79 5.32e-34 112
Msa1347060 MtrunA17_Chr1g0155181 59.302 86 29 1 6 85 1 86 6.14e-33 109
Msa1347060 MtrunA17_Chr3g0141601 57.143 91 35 2 6 92 1 91 3.14e-32 107
Msa1347060 MtrunA17_Chr5g0404101 63.014 73 27 0 4 76 11 83 1.24e-30 103
Msa1347060 MtrunA17_Chr8g0373131 56.716 67 28 1 9 75 2 67 7.81e-25 88.6
Msa1347060 MtrunA17_Chr8g0373111 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
Msa1347060 MtrunA17_Chr8g0373121 56.716 67 28 1 9 75 2 67 8.21e-25 88.6
Msa1347060 MtrunA17_Chr3g0141641 45.055 91 41 2 11 92 2 92 3.32e-24 87.4
Msa1347060 MtrunA17_Chr5g0404301 60.317 63 24 1 10 72 2 63 9.33e-24 85.5
Msa1347060 MtrunA17_Chr8g0373101 56.452 62 26 1 11 72 4 64 6.78e-23 83.6
Msa1347060 MtrunA17_Chr5g0436501 54.930 71 32 0 12 82 27 97 1.30e-20 82.8
Msa1347060 MtrunA17_Chr8g0364741 50.667 75 37 0 6 80 4 78 2.62e-20 78.6
Msa1347060 MtrunA17_Chr1g0162081 50.000 78 39 0 1 78 1 78 1.38e-19 80.5
Msa1347060 MtrunA17_Chr8g0365011 54.167 72 33 0 9 80 5 76 2.84e-19 79.3
Msa1347060 MtrunA17_Chr3g0104071 50.685 73 36 0 2 74 33 105 9.65e-19 78.2
Msa1347060 MtrunA17_Chr5g0429521 47.826 69 36 0 11 79 28 96 5.02e-18 76.3
Msa1347060 MtrunA17_Chr7g0241761 52.174 69 33 0 12 80 35 103 5.19e-18 76.3
Msa1347060 MtrunA17_Chr1g0194071 57.627 59 24 1 11 68 10 68 6.75e-16 66.6
Msa1347060 MtrunA17_Chr1g0210471 47.619 63 33 0 12 74 24 86 7.82e-15 67.0
Msa1347060 MtrunA17_Chr7g0270711 55.000 60 26 1 11 69 10 69 3.19e-14 62.0
Msa1347060 MtrunA17_Chr4g0062741 45.205 73 36 2 6 74 1 73 4.56e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1347060 AT2G21650.1 64.773 88 24 1 12 92 14 101 4.16e-38 123
Msa1347060 AT4G39250.1 64.368 87 25 1 12 92 14 100 1.32e-37 122
Msa1347060 AT1G75250.2 55.670 97 37 1 1 91 1 97 5.60e-36 117
Msa1347060 AT1G75250.1 69.333 75 23 0 1 75 1 75 1.47e-35 117
Msa1347060 AT1G19510.1 59.783 92 37 0 1 92 1 92 2.64e-29 100
Msa1347060 AT2G18328.1 62.295 61 23 0 15 75 14 74 1.84e-24 88.2
Msa1347060 AT5G05790.2 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
Msa1347060 AT5G05790.1 52.857 70 33 0 9 78 28 97 3.54e-20 82.0
Msa1347060 AT4G36570.1 75.000 44 11 0 12 55 12 55 4.92e-20 76.3
Msa1347060 AT3G11280.2 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
Msa1347060 AT3G11280.1 52.239 67 32 0 12 78 33 99 1.03e-18 77.8
Msa1347060 AT2G38090.1 52.174 69 33 0 12 80 29 97 2.27e-18 77.4
Msa1347060 AT5G58900.1 50.000 74 34 1 4 74 23 96 3.03e-17 74.3
Msa1347060 AT5G08520.1 52.381 63 30 0 12 74 12 74 5.44e-16 70.9
Msa1347060 AT1G49010.1 46.479 71 35 1 7 74 3 73 1.43e-14 67.4
Msa1347060 AT5G23650.1 46.377 69 37 0 6 74 7 75 2.01e-14 67.0
Msa1347060 AT5G04760.1 41.791 67 38 1 8 74 2 67 4.55e-13 62.4
Msa1347060 AT5G01200.1 42.424 66 37 1 10 74 28 93 2.05e-11 58.2

Find 33 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCTAGTATCCTAATTAA+TGG 0.186484 tig0009457:+13657 Msa1347060:three_prime_UTR
AACAATTAATAAGCTAGTTT+TGG 0.255773 tig0009457:-13107 Msa1347060:intergenic
ACCCCTCAATACCATTAATT+AGG 0.339309 tig0009457:-13668 Msa1347060:intergenic
TACTTTCTTGTTGTTGAAGC+AGG 0.354538 tig0009457:+13798 Msa1347060:three_prime_UTR
TCTAGTTCAATGTCAGCTTC+TGG 0.381239 tig0009457:+13163 Msa1347060:CDS
GATATTAAACATATTGAGTC+AGG 0.394581 tig0009457:+13334 Msa1347060:CDS
TTGTTGTTGATTGCACTTGT+TGG 0.397526 tig0009457:-13620 Msa1347060:intergenic
TAATGTTTCTTAACTTCCTC+AGG 0.405406 tig0009457:-13295 Msa1347060:intergenic
GCCATTTCTTAATTAATTCA+AGG 0.418850 tig0009457:-13139 Msa1347060:intergenic
ACCTTGAATTAATTAAGAAA+TGG 0.425549 tig0009457:+13138 Msa1347060:exon
ATCCTAATTAATGGTATTGA+GGG 0.440615 tig0009457:+13666 Msa1347060:three_prime_UTR
CAGTGTCTCATGAGGAGAAA+AGG 0.467404 tig0009457:+13389 Msa1347060:CDS
TATCCTAATTAATGGTATTG+AGG 0.477146 tig0009457:+13665 Msa1347060:three_prime_UTR
CAATGTCAGCTTCTGGCTCA+TGG 0.480309 tig0009457:+13170 Msa1347060:CDS
TTCTGGCTCATGGAGTGTTA+AGG 0.488975 tig0009457:+13180 Msa1347060:CDS
TTGCACTTGTTGGTCAATGA+AGG 0.513934 tig0009457:-13610 Msa1347060:intergenic
GCTACATTGTACCAACGATC+TGG 0.520197 tig0009457:-13250 Msa1347060:intergenic
TAATTAATTCAAGGTATGAA+AGG 0.527947 tig0009457:-13130 Msa1347060:intergenic
CACTTATGAAGAGTGGTAAG+TGG 0.542822 tig0009457:-13075 Msa1347060:intergenic
CTTCAACAACAAGAAAGTAA+AGG 0.546066 tig0009457:-13794 Msa1347060:intergenic
AACAGCTAAAGCTCTTTCAA+AGG 0.549456 tig0009457:-13212 Msa1347060:intergenic
ACATTATGAACTTCTTGTTG+AGG 0.570098 tig0009457:+13312 Msa1347060:CDS
AACAACAAGAAAGTAAAGGA+TGG 0.573425 tig0009457:-13790 Msa1347060:intergenic
GAAAATTTCAGTGTCTCATG+AGG 0.590467 tig0009457:+13381 Msa1347060:CDS
TACAATGTAGCTCATGCTGT+TGG 0.593737 tig0009457:+13262 Msa1347060:CDS
TGAGAATCACTTATGAAGAG+TGG 0.595892 tig0009457:-13082 Msa1347060:intergenic
AATGTAGCTCATGCTGTTGG+TGG 0.602491 tig0009457:+13265 Msa1347060:CDS
ACAAAGACACTCCAGATCGT+TGG 0.612332 tig0009457:+13239 Msa1347060:CDS
CAACTTTATTAAGAACGAGG+AGG 0.657197 tig0009457:-13845 Msa1347060:intergenic
ATATTAAACATATTGAGTCA+GGG 0.667795 tig0009457:+13335 Msa1347060:CDS
TCCTAATTAATGGTATTGAG+GGG 0.676186 tig0009457:+13667 Msa1347060:three_prime_UTR
TTACAACTTTATTAAGAACG+AGG 0.684701 tig0009457:-13848 Msa1347060:intergenic
TGTTGGTGGCAAAACTCCTG+AGG 0.702099 tig0009457:+13279 Msa1347060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAATTAATAAGCTAGTTT+TGG - tig0009457:13110-13129 None:intergenic 20.0%
!! TAATTAATTCAAGGTATGAA+AGG - tig0009457:13133-13152 None:intergenic 20.0%
!! ACCTTGAATTAATTAAGAAA+TGG + tig0009457:13138-13157 Msa1347060:exon 20.0%
!! ATATTAAACATATTGAGTCA+GGG + tig0009457:13335-13354 Msa1347060:CDS 20.0%
!!! ATTTTCTTGTAATTTGGAAA+TGG - tig0009457:13367-13386 Msa1347060:intergenic 20.0%
!!! ACTGAAATTTTCTTGTAATT+TGG - tig0009457:13373-13392 Msa1347060:intergenic 20.0%
!! TTTAATTATTTAACAAGCGT+AGG - tig0009457:13460-13479 Msa1347060:intergenic 20.0%
!!! TACTCTTTACTTTCTTTTAT+AGG + tig0009457:13573-13592 Msa1347060:intron 20.0%
!! TATTCTAGTATCCTAATTAA+TGG + tig0009457:13657-13676 Msa1347060:three_prime_UTR 20.0%
!!! ATTTTATCCAAAAAGAGTAA+AGG - tig0009457:13769-13788 Msa1347060:intergenic 20.0%
!!! AACAATTAATAAGCTAGTTT+TGG - tig0009457:13110-13129 Msa1347060:intergenic 20.0%
!! TAATTAATTCAAGGTATGAA+AGG - tig0009457:13133-13152 Msa1347060:intergenic 20.0%
!! ACCTTGAATTAATTAAGAAA+TGG + tig0009457:13138-13157 Msa1347060:exon 20.0%
!! ATATTAAACATATTGAGTCA+GGG + tig0009457:13335-13354 Msa1347060:CDS 20.0%
!!! ATTTTCTTGTAATTTGGAAA+TGG - tig0009457:13367-13386 Msa1347060:intergenic 20.0%
!!! ACTGAAATTTTCTTGTAATT+TGG - tig0009457:13373-13392 Msa1347060:intergenic 20.0%
!! TTTAATTATTTAACAAGCGT+AGG - tig0009457:13460-13479 Msa1347060:intergenic 20.0%
!!! TACTCTTTACTTTCTTTTAT+AGG + tig0009457:13573-13592 Msa1347060:intron 20.0%
!! TATTCTAGTATCCTAATTAA+TGG + tig0009457:13657-13676 Msa1347060:three_prime_UTR 20.0%
!!! ATTTTATCCAAAAAGAGTAA+AGG - tig0009457:13769-13788 Msa1347060:intergenic 20.0%
! GCCATTTCTTAATTAATTCA+AGG - tig0009457:13142-13161 Msa1347060:intergenic 25.0%
! GATATTAAACATATTGAGTC+AGG + tig0009457:13334-13353 Msa1347060:CDS 25.0%
!! TTACTTTCTTTTATAGGATG+AGG + tig0009457:13579-13598 Msa1347060:intron 25.0%
! TATCCTAATTAATGGTATTG+AGG + tig0009457:13665-13684 Msa1347060:three_prime_UTR 25.0%
!! ATCCTAATTAATGGTATTGA+GGG + tig0009457:13666-13685 Msa1347060:three_prime_UTR 25.0%
!! ATTCTATCCTTTACTCTTTT+TGG + tig0009457:13759-13778 Msa1347060:three_prime_UTR 25.0%
! TTACAACTTTATTAAGAACG+AGG - tig0009457:13851-13870 Msa1347060:intergenic 25.0%
! GCCATTTCTTAATTAATTCA+AGG - tig0009457:13142-13161 Msa1347060:intergenic 25.0%
! GATATTAAACATATTGAGTC+AGG + tig0009457:13334-13353 Msa1347060:CDS 25.0%
!! TTACTTTCTTTTATAGGATG+AGG + tig0009457:13579-13598 Msa1347060:intron 25.0%
! TATCCTAATTAATGGTATTG+AGG + tig0009457:13665-13684 Msa1347060:three_prime_UTR 25.0%
!! ATCCTAATTAATGGTATTGA+GGG + tig0009457:13666-13685 Msa1347060:three_prime_UTR 25.0%
!! ATTCTATCCTTTACTCTTTT+TGG + tig0009457:13759-13778 Msa1347060:three_prime_UTR 25.0%
! TTACAACTTTATTAAGAACG+AGG - tig0009457:13851-13870 Msa1347060:intergenic 25.0%
TAATGTTTCTTAACTTCCTC+AGG - tig0009457:13298-13317 Msa1347060:intergenic 30.0%
ACATTATGAACTTCTTGTTG+AGG + tig0009457:13312-13331 Msa1347060:CDS 30.0%
! TCCTAATTAATGGTATTGAG+GGG + tig0009457:13667-13686 Msa1347060:three_prime_UTR 30.0%
AACAACAAGAAAGTAAAGGA+TGG - tig0009457:13793-13812 Msa1347060:intergenic 30.0%
CTTCAACAACAAGAAAGTAA+AGG - tig0009457:13797-13816 Msa1347060:intergenic 30.0%
TAATGTTTCTTAACTTCCTC+AGG - tig0009457:13298-13317 Msa1347060:intergenic 30.0%
ACATTATGAACTTCTTGTTG+AGG + tig0009457:13312-13331 Msa1347060:CDS 30.0%
! TCCTAATTAATGGTATTGAG+GGG + tig0009457:13667-13686 Msa1347060:three_prime_UTR 30.0%
AACAACAAGAAAGTAAAGGA+TGG - tig0009457:13793-13812 Msa1347060:intergenic 30.0%
CTTCAACAACAAGAAAGTAA+AGG - tig0009457:13797-13816 Msa1347060:intergenic 30.0%
TGAGAATCACTTATGAAGAG+TGG - tig0009457:13085-13104 None:intergenic 35.0%
AACAGCTAAAGCTCTTTCAA+AGG - tig0009457:13215-13234 Msa1347060:intergenic 35.0%
GAAAATTTCAGTGTCTCATG+AGG + tig0009457:13381-13400 Msa1347060:CDS 35.0%
! TTGTTGTTGATTGCACTTGT+TGG - tig0009457:13623-13642 Msa1347060:intergenic 35.0%
ACCCCTCAATACCATTAATT+AGG - tig0009457:13671-13690 Msa1347060:intergenic 35.0%
!! TACTTTCTTGTTGTTGAAGC+AGG + tig0009457:13798-13817 Msa1347060:three_prime_UTR 35.0%
CAACTTTATTAAGAACGAGG+AGG - tig0009457:13848-13867 Msa1347060:intergenic 35.0%
TGAGAATCACTTATGAAGAG+TGG - tig0009457:13085-13104 Msa1347060:intergenic 35.0%
AACAGCTAAAGCTCTTTCAA+AGG - tig0009457:13215-13234 Msa1347060:intergenic 35.0%
GAAAATTTCAGTGTCTCATG+AGG + tig0009457:13381-13400 Msa1347060:CDS 35.0%
! TTGTTGTTGATTGCACTTGT+TGG - tig0009457:13623-13642 Msa1347060:intergenic 35.0%
ACCCCTCAATACCATTAATT+AGG - tig0009457:13671-13690 Msa1347060:intergenic 35.0%
!! TACTTTCTTGTTGTTGAAGC+AGG + tig0009457:13798-13817 Msa1347060:three_prime_UTR 35.0%
CAACTTTATTAAGAACGAGG+AGG - tig0009457:13848-13867 Msa1347060:intergenic 35.0%
CACTTATGAAGAGTGGTAAG+TGG - tig0009457:13078-13097 None:intergenic 40.0%
! TCTAGTTCAATGTCAGCTTC+TGG + tig0009457:13163-13182 Msa1347060:CDS 40.0%
TACAATGTAGCTCATGCTGT+TGG + tig0009457:13262-13281 Msa1347060:CDS 40.0%
! TTGCACTTGTTGGTCAATGA+AGG - tig0009457:13613-13632 Msa1347060:intergenic 40.0%
CACTTATGAAGAGTGGTAAG+TGG - tig0009457:13078-13097 Msa1347060:intergenic 40.0%
! TCTAGTTCAATGTCAGCTTC+TGG + tig0009457:13163-13182 Msa1347060:CDS 40.0%
TACAATGTAGCTCATGCTGT+TGG + tig0009457:13262-13281 Msa1347060:CDS 40.0%
! TTGCACTTGTTGGTCAATGA+AGG - tig0009457:13613-13632 Msa1347060:intergenic 40.0%
!! TTCTGGCTCATGGAGTGTTA+AGG + tig0009457:13180-13199 Msa1347060:CDS 45.0%
ACAAAGACACTCCAGATCGT+TGG + tig0009457:13239-13258 Msa1347060:CDS 45.0%
GCTACATTGTACCAACGATC+TGG - tig0009457:13253-13272 Msa1347060:intergenic 45.0%
AATGTAGCTCATGCTGTTGG+TGG + tig0009457:13265-13284 Msa1347060:CDS 45.0%
CAGTGTCTCATGAGGAGAAA+AGG + tig0009457:13389-13408 Msa1347060:CDS 45.0%
ATGAAGAGTGGTAAGTGGAG+AGG - tig0009457:13073-13092 Msa1347060:intergenic 45.0%
!! TTCTGGCTCATGGAGTGTTA+AGG + tig0009457:13180-13199 Msa1347060:CDS 45.0%
ACAAAGACACTCCAGATCGT+TGG + tig0009457:13239-13258 Msa1347060:CDS 45.0%
GCTACATTGTACCAACGATC+TGG - tig0009457:13253-13272 Msa1347060:intergenic 45.0%
AATGTAGCTCATGCTGTTGG+TGG + tig0009457:13265-13284 Msa1347060:CDS 45.0%
CAGTGTCTCATGAGGAGAAA+AGG + tig0009457:13389-13408 Msa1347060:CDS 45.0%
!! CAATGTCAGCTTCTGGCTCA+TGG + tig0009457:13170-13189 Msa1347060:CDS 50.0%
!! TGTTGGTGGCAAAACTCCTG+AGG + tig0009457:13279-13298 Msa1347060:CDS 50.0%
!! CAATGTCAGCTTCTGGCTCA+TGG + tig0009457:13170-13189 Msa1347060:CDS 50.0%
!! TGTTGGTGGCAAAACTCCTG+AGG + tig0009457:13279-13298 Msa1347060:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0009457 gene 13072 13972 13072 ID=Msa1347060;Name=Msa1347060
tig0009457 mRNA 13072 13972 13072 ID=Msa1347060-mRNA-1;Parent=Msa1347060;Name=Msa1347060-mRNA-1;_AED=0.01;_eAED=0.01;_QI=85|1|1|1|1|1|2|353|92
tig0009457 exon 13072 13410 13072 ID=Msa1347060-mRNA-1:exon:5781;Parent=Msa1347060-mRNA-1
tig0009457 exon 13595 13972 13595 ID=Msa1347060-mRNA-1:exon:5782;Parent=Msa1347060-mRNA-1
tig0009457 five_prime_UTR 13072 13156 13072 ID=Msa1347060-mRNA-1:five_prime_utr;Parent=Msa1347060-mRNA-1
tig0009457 CDS 13157 13410 13157 ID=Msa1347060-mRNA-1:cds;Parent=Msa1347060-mRNA-1
tig0009457 CDS 13595 13619 13595 ID=Msa1347060-mRNA-1:cds;Parent=Msa1347060-mRNA-1
tig0009457 three_prime_UTR 13620 13972 13620 ID=Msa1347060-mRNA-1:three_prime_utr;Parent=Msa1347060-mRNA-1
Gene Sequence

>Msa1347060

ATGGCATCTAGTTCAATGTCAGCTTCTGGCTCATGGAGTGTTAAGGAGAACAAAGCCTTTGAAAGAGCTTTAGCTGTTTATGACAAAGACACTCCAGATCGTTGGTACAATGTAGCTCATGCTGTTGGTGGCAAAACTCCTGAGGAAGTTAAGAAACATTATGAACTTCTTGTTGAGGATATTAAACATATTGAGTCAGGGAAAGTTCCATTTCCAAATTACAAGAAAATTTCAGTGTCTCATGAGGAGAAAAGGATGAGGAACATGAGCCTTCATTGA

Protein sequence

>Msa1347060

MASSSMSASGSWSVKENKAFERALAVYDKDTPDRWYNVAHAVGGKTPEEVKKHYELLVEDIKHIESGKVPFPNYKKISVSHEEKRMRNMSLH*