Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0752570 | G7K9C1 | 93.814 | 97 | 5 | 1 | 1 | 97 | 1 | 96 | 6.04e-56 | 177 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0686830 | Msa0752570 | 0.803339 | 3.655156e-49 | -8.615850e-47 |
| Msa0484280 | Msa0752570 | 0.879659 | 1.040197e-69 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0752570 | MtrunA17_Chr5g0396641 | 93.814 | 97 | 5 | 1 | 1 | 97 | 1 | 96 | 1.16e-59 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTGATCTCAAGCATTTC+AGG | 0.222831 | 5_2:-6296849 | None:intergenic |
| TAACAAAAGAGGAGCTTAAA+TGG | 0.305453 | 5_2:+6296706 | Msa0752570:CDS |
| CACTATGAGGCCAGTGGAGA+TGG | 0.430323 | 5_2:+6296602 | Msa0752570:CDS |
| TCATCAATTAATGATACTAA+AGG | 0.445970 | 5_2:+6296654 | Msa0752570:CDS |
| TCGAAAATGCTCTCTAATGA+AGG | 0.448930 | 5_2:-6296822 | None:intergenic |
| TGTTGTTGCTCCATCTCCAC+TGG | 0.456318 | 5_2:-6296612 | None:intergenic |
| CATCAATTAATGATACTAAA+GGG | 0.470382 | 5_2:+6296655 | Msa0752570:CDS |
| AATTGATCTCAAGCATTTCA+GGG | 0.481268 | 5_2:-6296848 | None:intergenic |
| GAAAATTGTACTAACAAAAG+AGG | 0.510037 | 5_2:+6296695 | Msa0752570:CDS |
| GTGCTTGAAGAGATAGAGAA+AGG | 0.542667 | 5_2:+6296774 | Msa0752570:CDS |
| ATATTTCAACTCAACAAGAA+AGG | 0.554722 | 5_2:+6296732 | Msa0752570:CDS |
| AGTAATCATGGGAAATTGCA+TGG | 0.557962 | 5_2:+6296572 | None:intergenic |
| CAAGAAAGGAGGAATGAAAG+TGG | 0.568740 | 5_2:+6296746 | Msa0752570:CDS |
| GCATGGAGACATACACTATG+AGG | 0.579359 | 5_2:+6296589 | Msa0752570:CDS |
| ATTGATCTCAAGCATTTCAG+GGG | 0.625182 | 5_2:-6296847 | None:intergenic |
| TTTCAACTCAACAAGAAAGG+AGG | 0.647403 | 5_2:+6296735 | Msa0752570:CDS |
| GACATACACTATGAGGCCAG+TGG | 0.675454 | 5_2:+6296596 | Msa0752570:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATCAATTAATGATACTAA+AGG | + | chr5_2:6296654-6296673 | Msa0752570:CDS | 20.0% |
| !! | CATCAATTAATGATACTAAA+GGG | + | chr5_2:6296655-6296674 | Msa0752570:CDS | 20.0% |
| ! | AATGATACTAAAGGGAAAAA+TGG | + | chr5_2:6296663-6296682 | Msa0752570:CDS | 25.0% |
| ! | GAAAATTGTACTAACAAAAG+AGG | + | chr5_2:6296695-6296714 | Msa0752570:CDS | 25.0% |
| ! | ATATTTCAACTCAACAAGAA+AGG | + | chr5_2:6296732-6296751 | Msa0752570:CDS | 25.0% |
| TAACAAAAGAGGAGCTTAAA+TGG | + | chr5_2:6296706-6296725 | Msa0752570:CDS | 30.0% | |
| AAAGGTAGACAAAAAGTTGA+GGG | + | chr5_2:6296792-6296811 | Msa0752570:CDS | 30.0% | |
| AATTGATCTCAAGCATTTCA+GGG | - | chr5_2:6296851-6296870 | None:intergenic | 30.0% | |
| TTTCAACTCAACAAGAAAGG+AGG | + | chr5_2:6296735-6296754 | Msa0752570:CDS | 35.0% | |
| GAAAGGTAGACAAAAAGTTG+AGG | + | chr5_2:6296791-6296810 | Msa0752570:CDS | 35.0% | |
| TCGAAAATGCTCTCTAATGA+AGG | - | chr5_2:6296825-6296844 | None:intergenic | 35.0% | |
| ATTGATCTCAAGCATTTCAG+GGG | - | chr5_2:6296850-6296869 | None:intergenic | 35.0% | |
| CAATTGATCTCAAGCATTTC+AGG | - | chr5_2:6296852-6296871 | None:intergenic | 35.0% | |
| CAAGAAAGGAGGAATGAAAG+TGG | + | chr5_2:6296746-6296765 | Msa0752570:CDS | 40.0% | |
| GTGCTTGAAGAGATAGAGAA+AGG | + | chr5_2:6296774-6296793 | Msa0752570:CDS | 40.0% | |
| GTAGACAAAAAGTTGAGGGA+TGG | + | chr5_2:6296796-6296815 | Msa0752570:CDS | 40.0% | |
| GCATGGAGACATACACTATG+AGG | + | chr5_2:6296589-6296608 | Msa0752570:CDS | 45.0% | |
| GACATACACTATGAGGCCAG+TGG | + | chr5_2:6296596-6296615 | Msa0752570:CDS | 50.0% | |
| TGTTGTTGCTCCATCTCCAC+TGG | - | chr5_2:6296615-6296634 | None:intergenic | 50.0% | |
| CACTATGAGGCCAGTGGAGA+TGG | + | chr5_2:6296602-6296621 | Msa0752570:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 6296579 | 6296872 | 6296579 | ID=Msa0752570;Name=Msa0752570 |
| chr5_2 | mRNA | 6296579 | 6296872 | 6296579 | ID=Msa0752570-mRNA-1;Parent=Msa0752570;Name=Msa0752570-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|97 |
| chr5_2 | exon | 6296579 | 6296872 | 6296579 | ID=Msa0752570-mRNA-1:exon:2656;Parent=Msa0752570-mRNA-1 |
| chr5_2 | CDS | 6296579 | 6296872 | 6296579 | ID=Msa0752570-mRNA-1:cds;Parent=Msa0752570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
