Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0484280 | XP_024639888.1 | 94.845 | 97 | 4 | 1 | 1 | 97 | 1 | 96 | 4.41e-56 | 178 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0484280 | G7K9C1 | 94.845 | 97 | 4 | 1 | 1 | 97 | 1 | 96 | 2.11e-56 | 178 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006080 | Msa0484280 | 0.802484 | 5.489043e-49 | -8.615850e-47 |
| Msa0101530 | Msa0484280 | 0.810455 | 1.142700e-50 | -8.615850e-47 |
| Msa0265650 | Msa0484280 | 0.809798 | 1.583136e-50 | -8.615850e-47 |
| Msa0303900 | Msa0484280 | 0.828317 | 9.614995e-55 | -8.615850e-47 |
| Msa0333770 | Msa0484280 | 0.816424 | 5.570245e-52 | -8.615850e-47 |
| Msa0484280 | Msa0490100 | 0.808355 | 3.225719e-50 | -8.615850e-47 |
| Msa0484280 | Msa0501080 | 0.803006 | 4.282868e-49 | -8.615850e-47 |
| Msa0484280 | Msa0647300 | 0.811777 | 5.908646e-51 | -8.615850e-47 |
| Msa0484280 | Msa0686830 | 0.816455 | 5.481684e-52 | -8.615850e-47 |
| Msa0484280 | Msa0752570 | 0.879659 | 1.040197e-69 | -8.615850e-47 |
| Msa0484280 | Msa0838210 | 0.805184 | 1.508826e-49 | -8.615850e-47 |
| Msa0484280 | Msa1034950 | 0.802817 | 4.686150e-49 | -8.615850e-47 |
| Msa0484280 | Msa1075010 | 0.802269 | 6.077993e-49 | -8.615850e-47 |
| Msa0484280 | Msa1094810 | 0.825998 | 3.452391e-54 | -8.615850e-47 |
| Msa0484280 | Msa1284020 | 0.804793 | 1.821592e-49 | -8.615850e-47 |
| Msa0484280 | Msa1322500 | 0.815629 | 8.384337e-52 | -8.615850e-47 |
| Msa0484280 | Msa1384700 | 0.800509 | 1.394156e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0484280 | MtrunA17_Chr5g0396641 | 94.845 | 97 | 4 | 1 | 1 | 97 | 1 | 96 | 4.06e-60 | 178 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTGATCTCAAGCATTTC+AGG | 0.222831 | 3_4:+1611524 | None:intergenic |
| TAACAAAAGAGGAGCTTAAA+TGG | 0.305453 | 3_4:-1611667 | Msa0484280:CDS |
| CACTATGAGGCCAGTGGAGA+TGG | 0.430323 | 3_4:-1611771 | Msa0484280:CDS |
| TCTAAAATGCTCTCTAATGA+AGG | 0.434702 | 3_4:+1611551 | None:intergenic |
| TCATCAATTAATGATACTAA+AGG | 0.445970 | 3_4:-1611719 | Msa0484280:CDS |
| TGTTGTTGCTCCATCTCCAC+TGG | 0.456318 | 3_4:+1611761 | None:intergenic |
| CATCAATTAATGATACTAAA+GGG | 0.470382 | 3_4:-1611718 | Msa0484280:CDS |
| AATTGATCTCAAGCATTTCA+GGG | 0.481268 | 3_4:+1611525 | None:intergenic |
| GAAAATTGTACTAACAAAAG+AGG | 0.510037 | 3_4:-1611678 | Msa0484280:CDS |
| GTGCTTGAAGAGATAGAGAA+AGG | 0.542667 | 3_4:-1611599 | Msa0484280:CDS |
| ATATTTCAACTCAACAAGAA+AGG | 0.554722 | 3_4:-1611641 | Msa0484280:CDS |
| AGTAATCATGGGAAATTGCA+TGG | 0.557962 | 3_4:-1611801 | None:intergenic |
| CAAGAAAGGAGGAATGAAAG+TGG | 0.568740 | 3_4:-1611627 | Msa0484280:CDS |
| GCATGGAGACATACACTATG+AGG | 0.579359 | 3_4:-1611784 | Msa0484280:CDS |
| ATTGATCTCAAGCATTTCAG+GGG | 0.625182 | 3_4:+1611526 | None:intergenic |
| TTTCAACTCAACAAGAAAGG+AGG | 0.647403 | 3_4:-1611638 | Msa0484280:CDS |
| GACATACACTATGAGGCCAG+TGG | 0.675454 | 3_4:-1611777 | Msa0484280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATCAATTAATGATACTAA+AGG | - | chr3_4:1611598-1611617 | Msa0484280:CDS | 20.0% |
| !! | CATCAATTAATGATACTAAA+GGG | - | chr3_4:1611599-1611618 | Msa0484280:CDS | 20.0% |
| ! | AATGATACTAAAGGGAAAAA+TGG | - | chr3_4:1611607-1611626 | Msa0484280:CDS | 25.0% |
| ! | GAAAATTGTACTAACAAAAG+AGG | - | chr3_4:1611639-1611658 | Msa0484280:CDS | 25.0% |
| ! | ATATTTCAACTCAACAAGAA+AGG | - | chr3_4:1611676-1611695 | Msa0484280:CDS | 25.0% |
| TAACAAAAGAGGAGCTTAAA+TGG | - | chr3_4:1611650-1611669 | Msa0484280:CDS | 30.0% | |
| AAAGGTAGACAAAAAGTTGA+GGG | - | chr3_4:1611736-1611755 | Msa0484280:CDS | 30.0% | |
| ! | TCTAAAATGCTCTCTAATGA+AGG | + | chr3_4:1611769-1611788 | None:intergenic | 30.0% |
| AATTGATCTCAAGCATTTCA+GGG | + | chr3_4:1611795-1611814 | None:intergenic | 30.0% | |
| TTTCAACTCAACAAGAAAGG+AGG | - | chr3_4:1611679-1611698 | Msa0484280:CDS | 35.0% | |
| GAAAGGTAGACAAAAAGTTG+AGG | - | chr3_4:1611735-1611754 | Msa0484280:CDS | 35.0% | |
| ATTGATCTCAAGCATTTCAG+GGG | + | chr3_4:1611794-1611813 | None:intergenic | 35.0% | |
| CAATTGATCTCAAGCATTTC+AGG | + | chr3_4:1611796-1611815 | None:intergenic | 35.0% | |
| CAAGAAAGGAGGAATGAAAG+TGG | - | chr3_4:1611690-1611709 | Msa0484280:CDS | 40.0% | |
| GTGCTTGAAGAGATAGAGAA+AGG | - | chr3_4:1611718-1611737 | Msa0484280:CDS | 40.0% | |
| GTAGACAAAAAGTTGAGGGA+TGG | - | chr3_4:1611740-1611759 | Msa0484280:CDS | 40.0% | |
| GCATGGAGACATACACTATG+AGG | - | chr3_4:1611533-1611552 | Msa0484280:CDS | 45.0% | |
| GACATACACTATGAGGCCAG+TGG | - | chr3_4:1611540-1611559 | Msa0484280:CDS | 50.0% | |
| TGTTGTTGCTCCATCTCCAC+TGG | + | chr3_4:1611559-1611578 | None:intergenic | 50.0% | |
| CACTATGAGGCCAGTGGAGA+TGG | - | chr3_4:1611546-1611565 | Msa0484280:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 1611523 | 1611816 | 1611523 | ID=Msa0484280;Name=Msa0484280 |
| chr3_4 | mRNA | 1611523 | 1611816 | 1611523 | ID=Msa0484280-mRNA-1;Parent=Msa0484280;Name=Msa0484280-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|97 |
| chr3_4 | exon | 1611523 | 1611816 | 1611523 | ID=Msa0484280-mRNA-1:exon:484;Parent=Msa0484280-mRNA-1 |
| chr3_4 | CDS | 1611523 | 1611816 | 1611523 | ID=Msa0484280-mRNA-1:cds;Parent=Msa0484280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
