AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0754160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0754160 MtrunA17_Chr5g0398801 81.457 151 0 1 1 123 1 151 3.62e-81 234
Msa0754160 MtrunA17_Chr4g0011261 77.483 151 6 1 1 123 1 151 1.53e-76 223
Msa0754160 MtrunA17_Chr4g0056921 75.497 151 9 1 1 123 1 151 2.53e-75 220
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0754160 AT4G00100.1 75.497 151 9 1 1 123 1 151 7.69e-76 221
Msa0754160 AT3G60770.1 74.834 151 10 1 1 123 1 151 7.52e-75 219

Find 67 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATACGCCCCATGGTTTC+TGG 0.160395 5_2:+7717543 None:intergenic
GGCTCATTCTTCATGCTTTC+CGG 0.179180 5_2:-7714995 Msa0754160:three_prime_UTR
ACATTTCAGTGTTGCGGTAT+AGG 0.253624 5_2:-7717581 Msa0754160:five_prime_UTR
TTGGTTGCTTAGAGCTTGTA+TGG 0.287863 5_2:-7715017 Msa0754160:exon
CGCCCCATGGTTTCTGGCTT+CGG 0.325578 5_2:+7717549 None:intergenic
ATGCTTGCTGATCGCTTAGA+AGG 0.330073 5_2:-7714703 Msa0754160:three_prime_UTR
CATTTCAGTGTTGCGGTATA+GGG 0.330127 5_2:-7717580 Msa0754160:five_prime_UTR
CTCTTGCTTGTGTTTGGGTT+GGG 0.345954 5_2:+7717659 None:intergenic
TTACAGAAAGGCAATACAAT+AGG 0.375684 5_2:+7714644 None:intergenic
GTGCAGCAACCCTCTGTTAC+CGG 0.381872 5_2:+7714976 None:intergenic
AGAAAATTGACTTGCTCCTC+TGG 0.385566 5_2:+7714613 None:intergenic
GGTTTCAATCCGCAAGCATT+TGG 0.402835 5_2:-7716421 Msa0754160:CDS
GTAACAGAGGGTTGCTGCAC+TGG 0.405636 5_2:-7714973 Msa0754160:three_prime_UTR
AGGTTTATCTAGAGACTTGA+TGG 0.413371 5_2:-7714930 Msa0754160:three_prime_UTR
ACTCTTGCTTGTGTTTGGGT+TGG 0.418402 5_2:+7717658 None:intergenic
CACGAAGAATAACACCGATC+TGG 0.418817 5_2:+7717234 None:intergenic
TATTTGCAAGTTTGCTAAGA+AGG 0.425487 5_2:-7717270 Msa0754160:CDS
AATTTATCCCTTGATTAACT+AGG 0.428497 5_2:-7717621 Msa0754160:five_prime_UTR
AAACCGCCTTCTTTATCAAA+TGG 0.432414 5_2:+7716439 None:intergenic
AACAAGGATGAGTCTGAACT+TGG 0.451307 5_2:+7716374 None:intergenic
TACAGATCCTCGGGAATCTC+AGG 0.472902 5_2:+7716462 None:intergenic
ATTTATCCCTTGATTAACTA+GGG 0.478848 5_2:-7717620 Msa0754160:five_prime_UTR
ATCAAATGGTACAGATCCTC+GGG 0.499985 5_2:+7716453 None:intergenic
ATACAAACCCTAGTTAATCA+AGG 0.505909 5_2:+7717613 None:intergenic
TGTTCCTCTCCAAATGCTTG+CGG 0.511208 5_2:+7716412 None:intergenic
ACGAAGAATAACACCGATCT+GGG 0.513161 5_2:+7717235 None:intergenic
ATGGGGCGTATGCATAGTAA+AGG 0.515615 5_2:-7717534 Msa0754160:CDS
TATCAAATGGTACAGATCCT+CGG 0.516683 5_2:+7716452 None:intergenic
ATATCTTTCTACTTTATCAA+AGG 0.517054 5_2:+7714879 None:intergenic
AAATCTTACTACTTCCAGAC+AGG 0.518633 5_2:+7716297 None:intergenic
ATTTGCAAGTTTGCTAAGAA+GGG 0.526647 5_2:-7717269 Msa0754160:CDS
ATCAACAACTGCCAGCACTT+TGG 0.526669 5_2:-7715036 Msa0754160:CDS
GAAGAGATCTTAAGCCAGCT+AGG 0.528242 5_2:+7717389 None:intergenic
TGCATTTACAAATGAAACAT+AGG 0.530773 5_2:-7714743 Msa0754160:three_prime_UTR
ACAAAAGAACTTCTCCTAGC+TGG 0.534882 5_2:-7717403 Msa0754160:CDS
CTGGAATGCTAATTACAGAA+AGG 0.537400 5_2:+7714632 None:intergenic
TAACAAAATAAGAAATTGCA+TGG 0.540677 5_2:+7714906 None:intergenic
TCTGTACCATTTGATAAAGA+AGG 0.540683 5_2:-7716445 Msa0754160:CDS
TGGTTGCTTAGAGCTTGTAT+GGG 0.548197 5_2:-7715016 Msa0754160:exon
ATACCGCAACACTGAAATGT+TGG 0.548248 5_2:+7717584 None:intergenic
ACAGCCGAAGCCAGAAACCA+TGG 0.553216 5_2:-7717553 Msa0754160:exon
TTACCAACATTTCAGTGTTG+CGG 0.553262 5_2:-7717587 Msa0754160:five_prime_UTR
GGTTTGACTCCTTCCCAGAT+CGG 0.553356 5_2:-7717248 Msa0754160:intron
TGGGGCGTATGCATAGTAAA+GGG 0.554130 5_2:-7717533 Msa0754160:intron
CAGCCGAAGCCAGAAACCAT+GGG 0.554217 5_2:-7717552 Msa0754160:exon
TGCTGCACTGGCAATCTGCA+AGG 0.555960 5_2:-7714961 Msa0754160:three_prime_UTR
GCTCTAAGCAACCAAAGTGC+TGG 0.557410 5_2:+7715025 None:intergenic
TCATGCTTTCCGGTAACAGA+GGG 0.562108 5_2:-7714985 Msa0754160:three_prime_UTR
TTTACTATGCATACGCCCCA+TGG 0.572915 5_2:+7717536 None:intergenic
TTCTGTAATTAGCATTCCAG+AGG 0.574683 5_2:-7714629 Msa0754160:three_prime_UTR
TACAAACCCTAGTTAATCAA+GGG 0.593495 5_2:+7717614 None:intergenic
CAATCCGCAAGCATTTGGAG+AGG 0.603826 5_2:-7716416 Msa0754160:CDS
GGTTTATCTAGAGACTTGAT+GGG 0.612983 5_2:-7714929 Msa0754160:three_prime_UTR
AGAATAACACCGATCTGGGA+AGG 0.617486 5_2:+7717239 None:intergenic
GTACCATTTGATAAAGAAGG+CGG 0.628741 5_2:-7716442 Msa0754160:CDS
TGTAATAGCGAGCAAGACGG+TGG 0.633533 5_2:+7716340 None:intergenic
TTCATGCTTTCCGGTAACAG+AGG 0.633583 5_2:-7714986 Msa0754160:three_prime_UTR
GGTGGATTCTGCTCTCAACA+AGG 0.636755 5_2:+7716358 None:intergenic
GCATTTGGAGAGGAACAGAA+AGG 0.640840 5_2:-7716406 Msa0754160:CDS
TCTTACTACTTCCAGACAGG+GGG 0.663544 5_2:+7716300 None:intergenic
ATCTTACTACTTCCAGACAG+GGG 0.663935 5_2:+7716299 None:intergenic
GCTTAAGATCTCTTCACCAG+AGG 0.674370 5_2:-7717381 Msa0754160:intron
TCTTGTAATAGCGAGCAAGA+CGG 0.678094 5_2:+7716337 None:intergenic
GGAGAGGAACAGAAAGGACA+AGG 0.687113 5_2:-7716400 Msa0754160:CDS
AATCTTACTACTTCCAGACA+GGG 0.689024 5_2:+7716298 None:intergenic
ACTTGCACCTGAGATTCCCG+AGG 0.712102 5_2:-7716469 Msa0754160:CDS
AGCCGAAGCCAGAAACCATG+GGG 0.712434 5_2:-7717551 Msa0754160:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTATTTATTCAAACA+GGG + chr5_2:7715161-7715180 None:intergenic 10.0%
!! ATTAAATTATTTATTCAAAC+AGG + chr5_2:7715162-7715181 None:intergenic 10.0%
!!! ACATTTCTATTTATATTTTT+TGG + chr5_2:7715361-7715380 None:intergenic 10.0%
!! AATATAAATAGAAATGTTTA+AGG - chr5_2:7715364-7715383 Msa0754160:intron 10.0%
!! ATATAAATAGAAATGTTTAA+GGG - chr5_2:7715365-7715384 Msa0754160:intron 10.0%
!!! AGAATTTCATATTTAAAATT+TGG + chr5_2:7715392-7715411 None:intergenic 10.0%
!!! TTATATTTTGAATTTGTTTC+AGG - chr5_2:7714984-7715003 Msa0754160:three_prime_UTR 15.0%
!! TAATGCATTAGTTAAATTTA+TGG + chr5_2:7716486-7716505 None:intergenic 15.0%
!! TAAATTTAACTAATGCATTA+TGG - chr5_2:7716486-7716505 Msa0754160:intron 15.0%
!! AAATTTAACTAATGCATTAT+GGG - chr5_2:7716487-7716506 Msa0754160:intron 15.0%
!!! TTTTTAAAATAGATGAAAAG+CGG - chr5_2:7716634-7716653 Msa0754160:intron 15.0%
!! CTTTACAATTGATAAAATAG+AGG - chr5_2:7715222-7715241 Msa0754160:intron 20.0%
!!! AATATATCCCATTTTTTTGA+GGG - chr5_2:7715427-7715446 Msa0754160:intron 20.0%
!!! TGCTATCTTAAATATTTTGA+AGG + chr5_2:7715471-7715490 None:intergenic 20.0%
!! GAAGATTACATATGTATTTA+GGG + chr5_2:7715635-7715654 None:intergenic 20.0%
!! TGAAGATTACATATGTATTT+AGG + chr5_2:7715636-7715655 None:intergenic 20.0%
!! AAAAAAAGTAGATAATGTTG+AGG - chr5_2:7715739-7715758 Msa0754160:intron 20.0%
!!! TCATTGTTTTGTTAATATGA+AGG - chr5_2:7715795-7715814 Msa0754160:intron 20.0%
!!! TTTCTGTCATTTATTTGAAA+GGG - chr5_2:7716389-7716408 Msa0754160:CDS 20.0%
!! TATGCTTATACTTATTTAGT+AGG + chr5_2:7716738-7716757 None:intergenic 20.0%
!! AACAGAGTAAACAATATAAT+TGG + chr5_2:7716821-7716840 None:intergenic 20.0%
!! AGCAATTTATATCAAAGTAA+TGG + chr5_2:7716873-7716892 None:intergenic 20.0%
!! TAACAAAATAAGAAATTGCA+TGG + chr5_2:7717384-7717403 None:intergenic 20.0%
!! ATATCTTTCTACTTTATCAA+AGG + chr5_2:7717411-7717430 None:intergenic 20.0%
!! TTAGAAGGAAAAATTTACAT+CGG - chr5_2:7717599-7717618 Msa0754160:five_prime_UTR 20.0%
! AATTTATCCCTTGATTAACT+AGG - chr5_2:7714666-7714685 Msa0754160:three_prime_UTR 25.0%
! ATTTATCCCTTGATTAACTA+GGG - chr5_2:7714667-7714686 Msa0754160:three_prime_UTR 25.0%
! CTCAAAAAAATGGGATATAT+TGG + chr5_2:7715428-7715447 None:intergenic 25.0%
!!! CAATATATCCCATTTTTTTG+AGG - chr5_2:7715426-7715445 Msa0754160:intron 25.0%
! TATTTGTCCCTCAAAAAAAT+GGG + chr5_2:7715437-7715456 None:intergenic 25.0%
! ATATTTGTCCCTCAAAAAAA+TGG + chr5_2:7715438-7715457 None:intergenic 25.0%
! AAATACAAAACAAATGGCAA+AGG + chr5_2:7716022-7716041 None:intergenic 25.0%
! GATGGAAAATACAAAACAAA+TGG + chr5_2:7716028-7716047 None:intergenic 25.0%
! TGACATAAACAAGGTTAATT+CGG + chr5_2:7716085-7716104 None:intergenic 25.0%
! CCAATAAAAAAATGGAATCT+AGG + chr5_2:7716285-7716304 None:intergenic 25.0%
!!! CCTAGATTCCATTTTTTTAT+TGG - chr5_2:7716282-7716301 Msa0754160:intron 25.0%
!!! ATTCCATTTTTTTATTGGCA+GGG - chr5_2:7716287-7716306 Msa0754160:intron 25.0%
! AAAAGGGAAGTAATAATCAA+AGG - chr5_2:7716329-7716348 Msa0754160:CDS 25.0%
! AAAGGGAAGTAATAATCAAA+GGG - chr5_2:7716330-7716349 Msa0754160:CDS 25.0%
! AAGTAATAATCAAAGGGTAA+AGG - chr5_2:7716336-7716355 Msa0754160:CDS 25.0%
!! CTTTCTGTCATTTATTTGAA+AGG - chr5_2:7716388-7716407 Msa0754160:CDS 25.0%
! ATGAACTCAAAATTAAAACG+TGG + chr5_2:7716583-7716602 None:intergenic 25.0%
!!! TTCGTTCTAAATTTTTACTC+AGG - chr5_2:7716766-7716785 Msa0754160:intron 25.0%
! AATATAATTGGTATGTGCAA+TGG + chr5_2:7716809-7716828 None:intergenic 25.0%
!! GCATAATATTGTTTCTCAAT+AGG - chr5_2:7717051-7717070 Msa0754160:intron 25.0%
! TTCTCAATAGGCTAATATTA+TGG - chr5_2:7717063-7717082 Msa0754160:intron 25.0%
! GTACATAAGAATAATAAACC+AGG + chr5_2:7717097-7717116 None:intergenic 25.0%
! TGCATTTACAAATGAAACAT+AGG - chr5_2:7717544-7717563 Msa0754160:exon 25.0%
! TAGAAGGAAAAATTTACATC+GGG - chr5_2:7717600-7717619 Msa0754160:five_prime_UTR 25.0%
! TTTTACTCTTGCTTGTGTTT+GGG + chr5_2:7714636-7714655 None:intergenic 30.0%
! TTTTTACTCTTGCTTGTGTT+TGG + chr5_2:7714637-7714656 None:intergenic 30.0%
TACAAACCCTAGTTAATCAA+GGG + chr5_2:7714676-7714695 None:intergenic 30.0%
ATACAAACCCTAGTTAATCA+AGG + chr5_2:7714677-7714696 None:intergenic 30.0%
GAAGAATGTTGAATTGAGAT+TGG + chr5_2:7714795-7714814 None:intergenic 30.0%
GTGAGAGAAATAAATTGAAG+AGG + chr5_2:7714817-7714836 None:intergenic 30.0%
!! ACCATTGATTTGATTCAGTA+AGG - chr5_2:7714837-7714856 Msa0754160:three_prime_UTR 30.0%
!! CCATTGATTTGATTCAGTAA+GGG - chr5_2:7714838-7714857 Msa0754160:three_prime_UTR 30.0%
AATGTGAAATTAGTTACCTC+TGG + chr5_2:7714925-7714944 None:intergenic 30.0%
!! TATTTGCAAGTTTGCTAAGA+AGG - chr5_2:7715017-7715036 Msa0754160:exon 30.0%
!! ATTTGCAAGTTTGCTAAGAA+GGG - chr5_2:7715018-7715037 Msa0754160:exon 30.0%
!! AAGCTGTTTTCACAAGTTAT+CGG - chr5_2:7715267-7715286 Msa0754160:intron 30.0%
AAGCACTTATGAGACTATTT+GGG + chr5_2:7715571-7715590 None:intergenic 30.0%
TAAGCACTTATGAGACTATT+TGG + chr5_2:7715572-7715591 None:intergenic 30.0%
!! TATTCATTTTAGTCACCACA+TGG + chr5_2:7715697-7715716 None:intergenic 30.0%
CAATGAATGAATAAACTCAG+CGG + chr5_2:7715781-7715800 None:intergenic 30.0%
TCTGTACCATTTGATAAAGA+AGG - chr5_2:7715842-7715861 Msa0754160:intron 30.0%
TTGTAGTTGAATCAATTGGA+TGG + chr5_2:7716046-7716065 None:intergenic 30.0%
GCATTTGTAGTTGAATCAAT+TGG + chr5_2:7716050-7716069 None:intergenic 30.0%
TTTGGCTAATGACATAAACA+AGG + chr5_2:7716094-7716113 None:intergenic 30.0%
CAAAGCTCAATATCGAAATT+TGG + chr5_2:7716112-7716131 None:intergenic 30.0%
AATTTCGATATTGAGCTTTG+TGG - chr5_2:7716112-7716131 Msa0754160:intron 30.0%
TTGAATTCGTTTACTTGTCT+AGG - chr5_2:7716190-7716209 Msa0754160:intron 30.0%
TGAATTCGTTTACTTGTCTA+GGG - chr5_2:7716191-7716210 Msa0754160:intron 30.0%
! TTTGGAATGCCTTAAAACTT+TGG - chr5_2:7716256-7716275 Msa0754160:intron 30.0%
TTGGAATGCCTTAAAACTTT+GGG - chr5_2:7716257-7716276 Msa0754160:intron 30.0%
!!! GATTCCATTTTTTTATTGGC+AGG - chr5_2:7716286-7716305 Msa0754160:intron 30.0%
TTTCCCTGCCAATAAAAAAA+TGG + chr5_2:7716293-7716312 None:intergenic 30.0%
TTTGAAAGGGTCATTCAAAT+TGG - chr5_2:7716402-7716421 Msa0754160:CDS 30.0%
TTGAAAGGGTCATTCAAATT+GGG - chr5_2:7716403-7716422 Msa0754160:CDS 30.0%
TTAAAACGTGGAAAACATGA+AGG + chr5_2:7716571-7716590 None:intergenic 30.0%
ATCAATTTCTGTTAATCACC+AGG - chr5_2:7716841-7716860 Msa0754160:intron 30.0%
TCAATTTCTGTTAATCACCA+GGG - chr5_2:7716842-7716861 Msa0754160:intron 30.0%
AGAATTGCAAATTAACAGGA+AGG + chr5_2:7717188-7717207 None:intergenic 30.0%
TGGTAGAATTGCAAATTAAC+AGG + chr5_2:7717192-7717211 None:intergenic 30.0%
TTACAGAAAGGCAATACAAT+AGG + chr5_2:7717646-7717665 None:intergenic 30.0%
TTACCAACATTTCAGTGTTG+CGG - chr5_2:7714700-7714719 Msa0754160:three_prime_UTR 35.0%
TGTTGAATTGAGATTGGAAG+AGG + chr5_2:7714789-7714808 None:intergenic 35.0%
CCCTTACTGAATCAAATCAA+TGG + chr5_2:7714841-7714860 None:intergenic 35.0%
! AGTTCTTTTGTAGGGTAAAG+CGG + chr5_2:7714875-7714894 None:intergenic 35.0%
! CTAGGAGAAGTTCTTTTGTA+GGG + chr5_2:7714883-7714902 None:intergenic 35.0%
GTTATTCTTCGTGATTCTCA+CGG - chr5_2:7715063-7715082 Msa0754160:intron 35.0%
!! AGCTGTTTTCACAAGTTATC+GGG - chr5_2:7715268-7715287 Msa0754160:intron 35.0%
TGAGGGACAAATATTAGAAC+AGG - chr5_2:7715444-7715463 Msa0754160:intron 35.0%
GAGGGACAAATATTAGAACA+GGG - chr5_2:7715445-7715464 Msa0754160:intron 35.0%
TATCAAATGGTACAGATCCT+CGG + chr5_2:7715838-7715857 None:intergenic 35.0%
GTACCATTTGATAAAGAAGG+CGG - chr5_2:7715845-7715864 Msa0754160:intron 35.0%
AAACCGCCTTCTTTATCAAA+TGG + chr5_2:7715851-7715870 None:intergenic 35.0%
AATCTTACTACTTCCAGACA+GGG + chr5_2:7715992-7716011 None:intergenic 35.0%
AAATCTTACTACTTCCAGAC+AGG + chr5_2:7715993-7716012 None:intergenic 35.0%
TTGATTCAACTACAAATGCC+AGG - chr5_2:7716051-7716070 Msa0754160:intron 35.0%
GAATTCGTTTACTTGTCTAG+GGG - chr5_2:7716192-7716211 Msa0754160:intron 35.0%
TGGAATGCCTTAAAACTTTG+GGG - chr5_2:7716258-7716277 Msa0754160:intron 35.0%
TTTATTGGCAGGGAAATATG+AGG - chr5_2:7716297-7716316 Msa0754160:intron 35.0%
ATATGAGGCATGTACACAAA+AGG - chr5_2:7716312-7716331 Msa0754160:CDS 35.0%
TATGAGGCATGTACACAAAA+GGG - chr5_2:7716313-7716332 Msa0754160:CDS 35.0%
AAAGACTTCAATGTCACTCT+TGG + chr5_2:7716691-7716710 None:intergenic 35.0%
GTAGGATAAAACACATACGA+AGG + chr5_2:7716720-7716739 None:intergenic 35.0%
TCAAAGTAATGGTAAAACCC+TGG + chr5_2:7716862-7716881 None:intergenic 35.0%
AATATTATGGTGTGATTGCC+TGG - chr5_2:7717076-7717095 Msa0754160:intron 35.0%
CAAAAGTCATTAGTTGCGAT+AGG + chr5_2:7717219-7717238 None:intergenic 35.0%
AGGTTTATCTAGAGACTTGA+TGG - chr5_2:7717357-7717376 Msa0754160:intron 35.0%
GGTTTATCTAGAGACTTGAT+GGG - chr5_2:7717358-7717377 Msa0754160:intron 35.0%
CTGGAATGCTAATTACAGAA+AGG + chr5_2:7717658-7717677 None:intergenic 35.0%
TTCTGTAATTAGCATTCCAG+AGG - chr5_2:7717658-7717677 Msa0754160:five_prime_UTR 35.0%
ATACCGCAACACTGAAATGT+TGG + chr5_2:7714706-7714725 None:intergenic 40.0%
ACATTTCAGTGTTGCGGTAT+AGG - chr5_2:7714706-7714725 Msa0754160:three_prime_UTR 40.0%
CATTTCAGTGTTGCGGTATA+GGG - chr5_2:7714707-7714726 Msa0754160:three_prime_UTR 40.0%
! GCTAGGAGAAGTTCTTTTGT+AGG + chr5_2:7714884-7714903 None:intergenic 40.0%
ACAAAAGAACTTCTCCTAGC+TGG - chr5_2:7714884-7714903 Msa0754160:three_prime_UTR 40.0%
ACGAAGAATAACACCGATCT+GGG + chr5_2:7715055-7715074 None:intergenic 40.0%
!! TACCATGAGCTTTGAGGATA+CGG + chr5_2:7715128-7715147 None:intergenic 40.0%
!! GCTGTTTTCACAAGTTATCG+GGG - chr5_2:7715269-7715288 Msa0754160:intron 40.0%
TTCAGCACCTCAAAATTTCG+AGG + chr5_2:7715334-7715353 None:intergenic 40.0%
!! ACCTGTTCGTAAGCTGTTTT+CGG + chr5_2:7715528-7715547 None:intergenic 40.0%
CCACATGGACACAAATTCAT+AGG + chr5_2:7715682-7715701 None:intergenic 40.0%
CCTATGAATTTGTGTCCATG+TGG - chr5_2:7715679-7715698 Msa0754160:intron 40.0%
ATCAAATGGTACAGATCCTC+GGG + chr5_2:7715837-7715856 None:intergenic 40.0%
AACAAGGATGAGTCTGAACT+TGG + chr5_2:7715916-7715935 None:intergenic 40.0%
TCTTGTAATAGCGAGCAAGA+CGG + chr5_2:7715953-7715972 None:intergenic 40.0%
ATCTTACTACTTCCAGACAG+GGG + chr5_2:7715991-7716010 None:intergenic 40.0%
! TTCGATATTGAGCTTTGTGG+TGG - chr5_2:7716115-7716134 Msa0754160:intron 40.0%
! TCTAGGTCCCCAAAGTTTTA+AGG + chr5_2:7716268-7716287 None:intergenic 40.0%
ATGGGTCCTAGTGAACTTTA+CGG + chr5_2:7716433-7716452 None:intergenic 40.0%
AACAAGATCCTTCTAAGTGC+AGG + chr5_2:7716461-7716480 None:intergenic 40.0%
CTAGTCCTCTCTGAATAGAA+CGG - chr5_2:7716517-7716536 Msa0754160:intron 40.0%
GTTAACCGTTCTATTCAGAG+AGG + chr5_2:7716525-7716544 None:intergenic 40.0%
GCACAGCAACATGAATGAAA+TGG - chr5_2:7716935-7716954 Msa0754160:intron 40.0%
AATTAACAGGAAGGCAAGAC+TGG + chr5_2:7717179-7717198 None:intergenic 40.0%
CATTAGTTGCGATAGGAACA+TGG + chr5_2:7717212-7717231 None:intergenic 40.0%
TTGGTTGCTTAGAGCTTGTA+TGG - chr5_2:7717270-7717289 Msa0754160:CDS 40.0%
TGGTTGCTTAGAGCTTGTAT+GGG - chr5_2:7717271-7717290 Msa0754160:CDS 40.0%
!! CAATCTGCAAGGTCATTTTG+AGG - chr5_2:7717337-7717356 Msa0754160:intron 40.0%
!! AGAAAATTGACTTGCTCCTC+TGG + chr5_2:7717677-7717696 None:intergenic 40.0%
! CTCTTGCTTGTGTTTGGGTT+GGG + chr5_2:7714631-7714650 None:intergenic 45.0%
ACTCTTGCTTGTGTTTGGGT+TGG + chr5_2:7714632-7714651 None:intergenic 45.0%
TTTACTATGCATACGCCCCA+TGG + chr5_2:7714754-7714773 None:intergenic 45.0%
ATGGGGCGTATGCATAGTAA+AGG - chr5_2:7714753-7714772 Msa0754160:three_prime_UTR 45.0%
TGGGGCGTATGCATAGTAAA+GGG - chr5_2:7714754-7714773 Msa0754160:three_prime_UTR 45.0%
! TCTTTTGTAGGGTAAAGCGG+AGG + chr5_2:7714872-7714891 None:intergenic 45.0%
GAAGAGATCTTAAGCCAGCT+AGG + chr5_2:7714901-7714920 None:intergenic 45.0%
GCTTAAGATCTCTTCACCAG+AGG - chr5_2:7714906-7714925 Msa0754160:three_prime_UTR 45.0%
AGAATAACACCGATCTGGGA+AGG + chr5_2:7715051-7715070 None:intergenic 45.0%
CACGAAGAATAACACCGATC+TGG + chr5_2:7715056-7715075 None:intergenic 45.0%
GAGAGATACCATGAGCTTTG+AGG + chr5_2:7715134-7715153 None:intergenic 45.0%
! TTTTCACAAGTTATCGGGGC+TGG - chr5_2:7715273-7715292 Msa0754160:intron 45.0%
! CACAAGGCCTCGAAATTTTG+AGG - chr5_2:7715324-7715343 Msa0754160:intron 45.0%
GATAGCATTGCAAGTTACGC+TGG - chr5_2:7715484-7715503 Msa0754160:intron 45.0%
GGTTTCAATCCGCAAGCATT+TGG - chr5_2:7715866-7715885 Msa0754160:intron 45.0%
TGTTCCTCTCCAAATGCTTG+CGG + chr5_2:7715878-7715897 None:intergenic 45.0%
GCATTTGGAGAGGAACAGAA+AGG - chr5_2:7715881-7715900 Msa0754160:intron 45.0%
TCTTACTACTTCCAGACAGG+GGG + chr5_2:7715990-7716009 None:intergenic 45.0%
! GTTAATTCGGTGACTAAGCC+TGG + chr5_2:7716072-7716091 None:intergenic 45.0%
TGGGTCCTAGTGAACTTTAC+GGG + chr5_2:7716432-7716451 None:intergenic 45.0%
TTCTAAGTGCAGGTGCTCAT+GGG + chr5_2:7716451-7716470 None:intergenic 45.0%
ATGAAGGAAGATGCACCCAA+GGG + chr5_2:7716555-7716574 None:intergenic 45.0%
GACTAGAGCTGAAGATGACA+CGG + chr5_2:7716607-7716626 None:intergenic 45.0%
GCAAGACTGGAAAGTTCAAG+AGG + chr5_2:7717166-7717185 None:intergenic 45.0%
ATCAACAACTGCCAGCACTT+TGG - chr5_2:7717251-7717270 Msa0754160:CDS 45.0%
GGCTCATTCTTCATGCTTTC+CGG - chr5_2:7717292-7717311 Msa0754160:intron 45.0%
TTCATGCTTTCCGGTAACAG+AGG - chr5_2:7717301-7717320 Msa0754160:intron 45.0%
TCATGCTTTCCGGTAACAGA+GGG - chr5_2:7717302-7717321 Msa0754160:intron 45.0%
! ATGCTTGCTGATCGCTTAGA+AGG - chr5_2:7717584-7717603 Msa0754160:five_prime_UTR 45.0%
!!! ATTAAAAATTAATTAAGTTT+TGG - chr5_2:7716238-7716257 Msa0754160:intron 5.0%
GGTTTGACTCCTTCCCAGAT+CGG - chr5_2:7715039-7715058 Msa0754160:CDS 50.0%
TGATTCTCACGGCATTGCTC+AGG - chr5_2:7715074-7715093 Msa0754160:intron 50.0%
!! CATGAGCTTTGAGGATACGG+AGG + chr5_2:7715125-7715144 None:intergenic 50.0%
CTCCGTATCCTCAAAGCTCA+TGG - chr5_2:7715123-7715142 Msa0754160:intron 50.0%
GCAAAGCGGGATACTACACA+AGG - chr5_2:7715308-7715327 Msa0754160:intron 50.0%
GCCGAAAACAGCTTACGAAC+AGG - chr5_2:7715524-7715543 Msa0754160:intron 50.0%
TACAGATCCTCGGGAATCTC+AGG + chr5_2:7715828-7715847 None:intergenic 50.0%
CAATCCGCAAGCATTTGGAG+AGG - chr5_2:7715871-7715890 Msa0754160:intron 50.0%
GGAGAGGAACAGAAAGGACA+AGG - chr5_2:7715887-7715906 Msa0754160:intron 50.0%
! GGTGGATTCTGCTCTCAACA+AGG + chr5_2:7715932-7715951 None:intergenic 50.0%
TGTAATAGCGAGCAAGACGG+TGG + chr5_2:7715950-7715969 None:intergenic 50.0%
GGCATCCCGTAAAGTTCACT+AGG - chr5_2:7716424-7716443 Msa0754160:CDS 50.0%
CTTCTAAGTGCAGGTGCTCA+TGG + chr5_2:7716452-7716471 None:intergenic 50.0%
CATGAGCACCTGCACTTAGA+AGG - chr5_2:7716450-7716469 Msa0754160:CDS 50.0%
ACGGTTAACATCCTTCCCCT+TGG - chr5_2:7716536-7716555 Msa0754160:intron 50.0%
CGGTTAACATCCTTCCCCTT+GGG - chr5_2:7716537-7716556 Msa0754160:intron 50.0%
TGAAGGAAGATGCACCCAAG+GGG + chr5_2:7716554-7716573 None:intergenic 50.0%
CATGAAGGAAGATGCACCCA+AGG + chr5_2:7716556-7716575 None:intergenic 50.0%
! TGTTGCTGTGCAGAAGCACA+CGG + chr5_2:7716926-7716945 None:intergenic 50.0%
! GCTCTAAGCAACCAAAGTGC+TGG + chr5_2:7717265-7717284 None:intergenic 50.0%
ACAGCCGAAGCCAGAAACCA+TGG - chr5_2:7714734-7714753 Msa0754160:three_prime_UTR 55.0%
CAGCCGAAGCCAGAAACCAT+GGG - chr5_2:7714735-7714754 Msa0754160:three_prime_UTR 55.0%
AGCCGAAGCCAGAAACCATG+GGG - chr5_2:7714736-7714755 Msa0754160:three_prime_UTR 55.0%
TGCATACGCCCCATGGTTTC+TGG + chr5_2:7714747-7714766 None:intergenic 55.0%
!! GCTCAGGTCAAGAGCGTTAC+TGG - chr5_2:7715090-7715109 Msa0754160:intron 55.0%
TGTAGTATCCCGCTTTGCGC+AGG + chr5_2:7715306-7715325 None:intergenic 55.0%
ACTTGCACCTGAGATTCCCG+AGG - chr5_2:7715818-7715837 Msa0754160:intron 55.0%
! GTTGCTGTGCAGAAGCACAC+GGG + chr5_2:7716925-7716944 None:intergenic 55.0%
GTGCAGCAACCCTCTGTTAC+CGG + chr5_2:7717314-7717333 None:intergenic 55.0%
GTAACAGAGGGTTGCTGCAC+TGG - chr5_2:7717314-7717333 Msa0754160:intron 55.0%
! TGCTGCACTGGCAATCTGCA+AGG - chr5_2:7717326-7717345 Msa0754160:intron 55.0%
!! CGCCCCATGGTTTCTGGCTT+CGG + chr5_2:7714741-7714760 None:intergenic 60.0%
GGTGAACAGCCTGCGCAAAG+CGG - chr5_2:7715294-7715313 Msa0754160:intron 60.0%
GTGAACAGCCTGCGCAAAGC+GGG - chr5_2:7715295-7715314 Msa0754160:intron 60.0%
CCAGACAGGGGGAAGCTTCT+TGG + chr5_2:7715979-7715998 None:intergenic 60.0%
CCAAGAAGCTTCCCCCTGTC+TGG - chr5_2:7715976-7715995 Msa0754160:intron 60.0%
GGAAGATGCACCCAAGGGGA+AGG + chr5_2:7716550-7716569 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 7714612 7717697 7714612 ID=Msa0754160;Name=Msa0754160
chr5_2 mRNA 7714612 7717697 7714612 ID=Msa0754160-mRNA-1;Parent=Msa0754160;Name=Msa0754160-mRNA-1;_AED=0.07;_eAED=0.07;_QI=141|0.75|0.8|1|0.75|0.8|5|416|123
chr5_2 exon 7714612 7715061 7714612 ID=Msa0754160-mRNA-1:exon:3522;Parent=Msa0754160-mRNA-1
chr5_2 exon 7716306 7716492 7716306 ID=Msa0754160-mRNA-1:exon:3521;Parent=Msa0754160-mRNA-1
chr5_2 exon 7717249 7717303 7717249 ID=Msa0754160-mRNA-1:exon:3520;Parent=Msa0754160-mRNA-1
chr5_2 exon 7717382 7717454 7717382 ID=Msa0754160-mRNA-1:exon:3519;Parent=Msa0754160-mRNA-1
chr5_2 exon 7717534 7717697 7717534 ID=Msa0754160-mRNA-1:exon:3518;Parent=Msa0754160-mRNA-1
chr5_2 five_prime_UTR 7717557 7717697 7717557 ID=Msa0754160-mRNA-1:five_prime_utr;Parent=Msa0754160-mRNA-1
chr5_2 CDS 7717534 7717556 7717534 ID=Msa0754160-mRNA-1:cds;Parent=Msa0754160-mRNA-1
chr5_2 CDS 7717382 7717454 7717382 ID=Msa0754160-mRNA-1:cds;Parent=Msa0754160-mRNA-1
chr5_2 CDS 7717249 7717303 7717249 ID=Msa0754160-mRNA-1:cds;Parent=Msa0754160-mRNA-1
chr5_2 CDS 7716306 7716492 7716306 ID=Msa0754160-mRNA-1:cds;Parent=Msa0754160-mRNA-1
chr5_2 CDS 7715028 7715061 7715028 ID=Msa0754160-mRNA-1:cds;Parent=Msa0754160-mRNA-1
chr5_2 three_prime_UTR 7714612 7715027 7714612 ID=Msa0754160-mRNA-1:three_prime_utr;Parent=Msa0754160-mRNA-1
Gene Sequence

>Msa0754160

ATGGGGCGTATGCATAGTAAAGGTAAGGGTATTTCTTCCTCCGCTTTACCCTACAAAAGAACTTCTCCTAGCTGGCTTAAGATCTCTTCACCAGAGGTTGATGAGACTATTTGCAAGTTTGCTAAGAAGGGTTTGACTCCTTCCCAGATCGGACTTGCACCTGAGATTCCCGAGGATCTGTACCATTTGATAAAGAAGGCGGTTTCAATCCGCAAGCATTTGGAGAGGAACAGAAAGGACAAGGACTCCAAGTTCAGACTCATCCTTGTTGAGAGCAGAATCCACCGTCTTGCTCGCTATTACAAGAAAACCAAGAAGCTTCCCCCTGTCTGGAAGTACGAATCAACAACTGCCAGCACTTTGGTTGCTTAG
Protein sequence

>Msa0754160

MGRMHSKGKGISSSALPYKRTSPSWLKISSPEVDETICKFAKKGLTPSQIGLAPEIPEDLYHLIKKAVSIRKHLERNRKDKDSKFRLILVESRIHRLARYYKKTKKLPPVWKYESTTASTLVA*