AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0580500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580500 MtrunA17_Chr5g0398801 84.106 151 0 1 1 127 1 151 1.73e-84 243
Msa0580500 MtrunA17_Chr4g0011261 80.132 151 6 1 1 127 1 151 8.33e-80 231
Msa0580500 MtrunA17_Chr4g0056921 78.146 151 9 1 1 127 1 151 1.05e-78 228
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580500 AT4G00100.1 78.146 151 9 1 1 127 1 151 3.12e-79 230
Msa0580500 AT3G60770.1 77.483 151 10 1 1 127 1 151 2.71e-78 228

Find 68 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATACGCCCCATGGTTTC+TGG 0.160395 4_1:+85599453 None:intergenic
GGCTCATTCTTCATGCTTTC+CGG 0.179180 4_1:-85596898 Msa0580500:three_prime_UTR
ACATTTCAGTGTTGCGGTAT+AGG 0.253624 4_1:-85599491 Msa0580500:five_prime_UTR
TTGGTTGCTTAGAGCTTGTA+TGG 0.287863 4_1:-85596920 Msa0580500:exon
ATGCTTGCTGATCGCTTAGA+AGG 0.322278 4_1:-85596603 Msa0580500:three_prime_UTR
CGCCCCATGGTTTCTGGCTT+CGG 0.325578 4_1:+85599459 None:intergenic
CATTTCAGTGTTGCGGTATA+GGG 0.330127 4_1:-85599490 Msa0580500:five_prime_UTR
CTCTTGCTTGTGTTTGGGTT+GGG 0.345954 4_1:+85599569 None:intergenic
TTACAGAAAGGCAATACAAT+AGG 0.375684 4_1:+85596543 None:intergenic
GTGCAGCAACCCTCTGTTAC+CGG 0.381872 4_1:+85596879 None:intergenic
AGAAAATTGACTTGCTCCTC+TGG 0.385566 4_1:+85596512 None:intergenic
GGTTTCAATCCGCAAGCATT+TGG 0.402835 4_1:-85598326 Msa0580500:CDS
GTAACAGAGGGTTGCTGCAC+TGG 0.405636 4_1:-85596876 Msa0580500:three_prime_UTR
AGGTTTATCTAGAGACTTGA+TGG 0.413371 4_1:-85596833 Msa0580500:three_prime_UTR
ACTCTTGCTTGTGTTTGGGT+TGG 0.418402 4_1:+85599568 None:intergenic
CACGAAGAATAACACCGATC+TGG 0.418817 4_1:+85599142 None:intergenic
TATTTGCAAGTTTGCTAAGA+AGG 0.425487 4_1:-85599178 Msa0580500:CDS
AATTTATCCCTTGATTAACT+AGG 0.428497 4_1:-85599531 Msa0580500:five_prime_UTR
AAACCGCCTTCTTTATCAAA+TGG 0.432414 4_1:+85598344 None:intergenic
AACAAGGATGAGTCTGAACT+TGG 0.451307 4_1:+85598279 None:intergenic
TACAGATCCTCGGGAATCTC+AGG 0.472902 4_1:+85598367 None:intergenic
ATTTATCCCTTGATTAACTA+GGG 0.478848 4_1:-85599530 Msa0580500:five_prime_UTR
ATCAAATGGTACAGATCCTC+GGG 0.499985 4_1:+85598358 None:intergenic
ATACAAACCCTAGTTAATCA+AGG 0.505909 4_1:+85599523 None:intergenic
TGTTCCTCTCCAAATGCTTG+CGG 0.511208 4_1:+85598317 None:intergenic
ACGAAGAATAACACCGATCT+GGG 0.513161 4_1:+85599143 None:intergenic
ATGGGGCGTATGCATAGTAA+AGG 0.515615 4_1:-85599444 Msa0580500:CDS
TATCAAATGGTACAGATCCT+CGG 0.516683 4_1:+85598357 None:intergenic
ATATCTTTCTACTTTATCAA+AGG 0.517054 4_1:+85596782 None:intergenic
AAATCTTACTACTTCCAGAC+AGG 0.518633 4_1:+85598202 None:intergenic
ATTTGCAAGTTTGCTAAGAA+GGG 0.526647 4_1:-85599177 Msa0580500:CDS
ATCAACAACTGCCAGCACTT+TGG 0.526669 4_1:-85596939 Msa0580500:CDS
GAAGAGATCTTAAGCCAGCT+AGG 0.528242 4_1:+85599297 None:intergenic
TGCATTTACAAATGAAACAT+AGG 0.530773 4_1:-85596643 Msa0580500:three_prime_UTR
ACAAAAGAACTTCTCCTAGC+TGG 0.534882 4_1:-85599311 Msa0580500:CDS
CTGGAATGCTAATTACAGAA+AGG 0.537400 4_1:+85596531 None:intergenic
TAACAAAATAAGAAATTGCA+TGG 0.540677 4_1:+85596809 None:intergenic
TCTGTACCATTTGATAAAGA+AGG 0.540683 4_1:-85598350 Msa0580500:CDS
TGGTTGCTTAGAGCTTGTAT+GGG 0.548197 4_1:-85596919 Msa0580500:exon
ATACCGCAACACTGAAATGT+TGG 0.548248 4_1:+85599494 None:intergenic
ACAGCCGAAGCCAGAAACCA+TGG 0.553216 4_1:-85599463 Msa0580500:exon
TTACCAACATTTCAGTGTTG+CGG 0.553262 4_1:-85599497 Msa0580500:five_prime_UTR
GGTTTGACTCCTTCCCAGAT+CGG 0.553356 4_1:-85599156 Msa0580500:CDS
TGGGGCGTATGCATAGTAAA+GGG 0.554130 4_1:-85599443 Msa0580500:intron
CAGCCGAAGCCAGAAACCAT+GGG 0.554217 4_1:-85599462 Msa0580500:exon
TGCTGCACTGGCAATCTGCA+AGG 0.555960 4_1:-85596864 Msa0580500:three_prime_UTR
GCTCTAAGCAACCAAAGTGC+TGG 0.557410 4_1:+85596928 None:intergenic
TCATGCTTTCCGGTAACAGA+GGG 0.562108 4_1:-85596888 Msa0580500:three_prime_UTR
TGCTTGCTGATCGCTTAGAA+GGG 0.562937 4_1:-85596602 Msa0580500:three_prime_UTR
TTTACTATGCATACGCCCCA+TGG 0.572915 4_1:+85599446 None:intergenic
TTCTGTAATTAGCATTCCAG+AGG 0.574683 4_1:-85596528 Msa0580500:three_prime_UTR
TACAAACCCTAGTTAATCAA+GGG 0.593495 4_1:+85599524 None:intergenic
CAATCCGCAAGCATTTGGAG+AGG 0.603826 4_1:-85598321 Msa0580500:CDS
GGTTTATCTAGAGACTTGAT+GGG 0.612983 4_1:-85596832 Msa0580500:three_prime_UTR
AGAATAACACCGATCTGGGA+AGG 0.617486 4_1:+85599147 None:intergenic
GTACCATTTGATAAAGAAGG+CGG 0.628741 4_1:-85598347 Msa0580500:CDS
TGTAATAGCGAGCAAGACGG+TGG 0.633533 4_1:+85598245 None:intergenic
TTCATGCTTTCCGGTAACAG+AGG 0.633583 4_1:-85596889 Msa0580500:three_prime_UTR
GGTGGATTCTGCTCTCAACA+AGG 0.636755 4_1:+85598263 None:intergenic
GCATTTGGAGAGGAACAGAA+AGG 0.640840 4_1:-85598311 Msa0580500:CDS
TCTTACTACTTCCAGACAGG+GGG 0.663544 4_1:+85598205 None:intergenic
ATCTTACTACTTCCAGACAG+GGG 0.663935 4_1:+85598204 None:intergenic
GCTTAAGATCTCTTCACCAG+AGG 0.674370 4_1:-85599289 Msa0580500:intron
TCTTGTAATAGCGAGCAAGA+CGG 0.678094 4_1:+85598242 None:intergenic
GGAGAGGAACAGAAAGGACA+AGG 0.687113 4_1:-85598305 Msa0580500:CDS
AATCTTACTACTTCCAGACA+GGG 0.689024 4_1:+85598203 None:intergenic
ACTTGCACCTGAGATTCCCG+AGG 0.712102 4_1:-85598374 Msa0580500:CDS
AGCCGAAGCCAGAAACCATG+GGG 0.712434 4_1:-85599461 Msa0580500:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTATTTATTCAAACA+GGG + chr4_1:85597062-85597081 None:intergenic 10.0%
!! ATTAAATTATTTATTCAAAC+AGG + chr4_1:85597063-85597082 None:intergenic 10.0%
!!! CATTTCTATTTTATATTTTT+TGG + chr4_1:85597263-85597282 None:intergenic 10.0%
!! ATATAAAATAGAAATGTTTA+AGG - chr4_1:85597267-85597286 Msa0580500:intron 10.0%
!! TATAAAATAGAAATGTTTAA+GGG - chr4_1:85597268-85597287 Msa0580500:intron 10.0%
!!! AGAATTTCATATTTAAAATT+TGG + chr4_1:85597295-85597314 None:intergenic 10.0%
!!! TTATATTTTGAATTTGTTTC+AGG - chr4_1:85596885-85596904 Msa0580500:three_prime_UTR 15.0%
!! TAATGCATTAGTTAAATTTA+TGG + chr4_1:85598393-85598412 None:intergenic 15.0%
!! TAAATTTAACTAATGCATTA+TGG - chr4_1:85598393-85598412 Msa0580500:intron 15.0%
!! AAATTTAACTAATGCATTAT+GGG - chr4_1:85598394-85598413 Msa0580500:intron 15.0%
!!! TTTTTAAAATAGATGAAAAG+CGG - chr4_1:85598541-85598560 Msa0580500:intron 15.0%
!! CTTTACAATTGATAAAATAG+AGG - chr4_1:85597123-85597142 Msa0580500:intron 20.0%
!!! AATATATCCCATTTTTTTGA+GGG - chr4_1:85597330-85597349 Msa0580500:intron 20.0%
!!! TGCTATCTTAAATATTTTGA+AGG + chr4_1:85597374-85597393 None:intergenic 20.0%
!! GAAGATTACATATGTATTTA+GGG + chr4_1:85597538-85597557 None:intergenic 20.0%
!! TGAAGATTACATATGTATTT+AGG + chr4_1:85597539-85597558 None:intergenic 20.0%
!! AAAAAAAGTAGATAATGTTG+AGG - chr4_1:85597643-85597662 Msa0580500:intron 20.0%
!!! TCATTGTTTTGTTAATATGA+AGG - chr4_1:85597699-85597718 Msa0580500:intron 20.0%
!!! TTTCTGTCATTTATTTGAAA+GGG - chr4_1:85598294-85598313 Msa0580500:CDS 20.0%
!! TATGCTTATACTTATTTAGT+AGG + chr4_1:85598645-85598664 None:intergenic 20.0%
!! AACAGAGTAAACAATATAAT+TGG + chr4_1:85598728-85598747 None:intergenic 20.0%
!! AGCAATTTATATCAAAGTAA+TGG + chr4_1:85598780-85598799 None:intergenic 20.0%
!! TACATAAGAAATAATAAACC+AGG + chr4_1:85599002-85599021 None:intergenic 20.0%
!! TAACAAAATAAGAAATTGCA+TGG + chr4_1:85599290-85599309 None:intergenic 20.0%
!! ATATCTTTCTACTTTATCAA+AGG + chr4_1:85599317-85599336 None:intergenic 20.0%
! AATTTATCCCTTGATTAACT+AGG - chr4_1:85596565-85596584 Msa0580500:three_prime_UTR 25.0%
! ATTTATCCCTTGATTAACTA+GGG - chr4_1:85596566-85596585 Msa0580500:three_prime_UTR 25.0%
! TGAGAGAAAATAAATTGAAG+AGG + chr4_1:85596716-85596735 None:intergenic 25.0%
!!! CATTGATTTGATTTCAGTAA+GGG - chr4_1:85596739-85596758 Msa0580500:three_prime_UTR 25.0%
! CTCAAAAAAATGGGATATAT+TGG + chr4_1:85597331-85597350 None:intergenic 25.0%
!!! CAATATATCCCATTTTTTTG+AGG - chr4_1:85597329-85597348 Msa0580500:intron 25.0%
! TATTTGTCCCTCAAAAAAAT+GGG + chr4_1:85597340-85597359 None:intergenic 25.0%
! ATATTTGTCCCTCAAAAAAA+TGG + chr4_1:85597341-85597360 None:intergenic 25.0%
! AAATACAAAACAAATGGCAA+AGG + chr4_1:85597926-85597945 None:intergenic 25.0%
! GATGGAAAATACAAAACAAA+TGG + chr4_1:85597932-85597951 None:intergenic 25.0%
! TGACATAAACAAGGTTAATT+CGG + chr4_1:85597989-85598008 None:intergenic 25.0%
! CCAATAAAAAAATGGAATCT+AGG + chr4_1:85598189-85598208 None:intergenic 25.0%
!!! CCTAGATTCCATTTTTTTAT+TGG - chr4_1:85598186-85598205 Msa0580500:intron 25.0%
!!! ATTCCATTTTTTTATTGGCA+GGG - chr4_1:85598191-85598210 Msa0580500:intron 25.0%
! AAAAGGGAAGTAATAATCAA+AGG - chr4_1:85598234-85598253 Msa0580500:CDS 25.0%
! AAAGGGAAGTAATAATCAAA+GGG - chr4_1:85598235-85598254 Msa0580500:CDS 25.0%
! AAGTAATAATCAAAGGGTAA+AGG - chr4_1:85598241-85598260 Msa0580500:CDS 25.0%
!! CTTTCTGTCATTTATTTGAA+AGG - chr4_1:85598293-85598312 Msa0580500:CDS 25.0%
! ATGAACTCAAAATTAAAACG+TGG + chr4_1:85598490-85598509 None:intergenic 25.0%
!!! TTCGTTCTAAATTTTTACTC+AGG - chr4_1:85598673-85598692 Msa0580500:intron 25.0%
! AATATAATTGGTATGTGCAA+TGG + chr4_1:85598716-85598735 None:intergenic 25.0%
!! GCATAATATTGTTTCTCAAT+AGG - chr4_1:85598956-85598975 Msa0580500:intron 25.0%
! TTCTCAATAGGCTAATATTA+TGG - chr4_1:85598968-85598987 Msa0580500:intron 25.0%
! TGCATTTACAAATGAAACAT+AGG - chr4_1:85599453-85599472 Msa0580500:exon 25.0%
! TAGAAGGGAAAAATTTACAT+CGG - chr4_1:85599509-85599528 Msa0580500:five_prime_UTR 25.0%
! TTTTACTCTTGCTTGTGTTT+GGG + chr4_1:85596535-85596554 None:intergenic 30.0%
! TTTTTACTCTTGCTTGTGTT+TGG + chr4_1:85596536-85596555 None:intergenic 30.0%
TACAAACCCTAGTTAATCAA+GGG + chr4_1:85596575-85596594 None:intergenic 30.0%
ATACAAACCCTAGTTAATCA+AGG + chr4_1:85596576-85596595 None:intergenic 30.0%
GAAGAATGTTGAATTGAGAT+TGG + chr4_1:85596694-85596713 None:intergenic 30.0%
CCTTACTGAAATCAAATCAA+TGG + chr4_1:85596741-85596760 None:intergenic 30.0%
!! CCATTGATTTGATTTCAGTA+AGG - chr4_1:85596738-85596757 Msa0580500:three_prime_UTR 30.0%
AATGTGAAATTAGTTACCTC+TGG + chr4_1:85596826-85596845 None:intergenic 30.0%
!! TATTTGCAAGTTTGCTAAGA+AGG - chr4_1:85596918-85596937 Msa0580500:exon 30.0%
!! ATTTGCAAGTTTGCTAAGAA+GGG - chr4_1:85596919-85596938 Msa0580500:exon 30.0%
!! AAGCTGTTTTCACAAGTTAT+CGG - chr4_1:85597169-85597188 Msa0580500:intron 30.0%
AAGCACTTATGAGACTATTT+GGG + chr4_1:85597474-85597493 None:intergenic 30.0%
TAAGCACTTATGAGACTATT+TGG + chr4_1:85597475-85597494 None:intergenic 30.0%
!!! ATTCATTTTTAGTCACCACA+TGG + chr4_1:85597600-85597619 None:intergenic 30.0%
CAATGAATGAATAAACTCAG+CGG + chr4_1:85597685-85597704 None:intergenic 30.0%
TCTGTACCATTTGATAAAGA+AGG - chr4_1:85597746-85597765 Msa0580500:intron 30.0%
TTGTAGTTGAATCAATTGGA+TGG + chr4_1:85597950-85597969 None:intergenic 30.0%
GCATTTGTAGTTGAATCAAT+TGG + chr4_1:85597954-85597973 None:intergenic 30.0%
TTTGGCTAATGACATAAACA+AGG + chr4_1:85597998-85598017 None:intergenic 30.0%
CAAAGCTCAATATCGAAATT+TGG + chr4_1:85598016-85598035 None:intergenic 30.0%
AATTTCGATATTGAGCTTTG+TGG - chr4_1:85598016-85598035 Msa0580500:intron 30.0%
TTGAATTCGTTTACTTGTCT+AGG - chr4_1:85598094-85598113 Msa0580500:intron 30.0%
TGAATTCGTTTACTTGTCTA+GGG - chr4_1:85598095-85598114 Msa0580500:intron 30.0%
! TTTGGAATGCCTTAAAACTT+TGG - chr4_1:85598160-85598179 Msa0580500:intron 30.0%
TTGGAATGCCTTAAAACTTT+GGG - chr4_1:85598161-85598180 Msa0580500:intron 30.0%
!!! GATTCCATTTTTTTATTGGC+AGG - chr4_1:85598190-85598209 Msa0580500:intron 30.0%
TTTCCCTGCCAATAAAAAAA+TGG + chr4_1:85598197-85598216 None:intergenic 30.0%
TATGAGGCATGTTTACAAAA+AGG - chr4_1:85598217-85598236 Msa0580500:CDS 30.0%
ATGAGGCATGTTTACAAAAA+GGG - chr4_1:85598218-85598237 Msa0580500:CDS 30.0%
TTGAAAGGGTCATTCAAATT+TGG - chr4_1:85598308-85598327 Msa0580500:CDS 30.0%
TGAAAGGGTCATTCAAATTT+GGG - chr4_1:85598309-85598328 Msa0580500:CDS 30.0%
TTAAAACGTGGAAAACATGA+AGG + chr4_1:85598478-85598497 None:intergenic 30.0%
ATCAATTTCTGTTAATCACC+AGG - chr4_1:85598748-85598767 Msa0580500:intron 30.0%
TCAATTTCTGTTAATCACCA+GGG - chr4_1:85598749-85598768 Msa0580500:intron 30.0%
AGAATTGCAAATTAACAGGA+AGG + chr4_1:85599094-85599113 None:intergenic 30.0%
TGGTAGAATTGCAAATTAAC+AGG + chr4_1:85599098-85599117 None:intergenic 30.0%
AGAAGGGAAAAATTTACATC+GGG - chr4_1:85599510-85599529 Msa0580500:five_prime_UTR 30.0%
TTACAGAAAGGCAATACAAT+AGG + chr4_1:85599556-85599575 None:intergenic 30.0%
TTACCAACATTTCAGTGTTG+CGG - chr4_1:85596599-85596618 Msa0580500:three_prime_UTR 35.0%
TGTTGAATTGAGATTGGAAG+AGG + chr4_1:85596688-85596707 None:intergenic 35.0%
! AGTTCTTTTGTAGGGTAAAG+CGG + chr4_1:85596776-85596795 None:intergenic 35.0%
! CTAGGAGAAGTTCTTTTGTA+GGG + chr4_1:85596784-85596803 None:intergenic 35.0%
GTTATTCTTCGTGATTCTCA+CGG - chr4_1:85596964-85596983 Msa0580500:intron 35.0%
!! AGCTGTTTTCACAAGTTATC+GGG - chr4_1:85597170-85597189 Msa0580500:intron 35.0%
TGAGGGACAAATATTAGAAC+AGG - chr4_1:85597347-85597366 Msa0580500:intron 35.0%
GAGGGACAAATATTAGAACA+GGG - chr4_1:85597348-85597367 Msa0580500:intron 35.0%
TATCAAATGGTACAGATCCT+CGG + chr4_1:85597742-85597761 None:intergenic 35.0%
GTACCATTTGATAAAGAAGG+CGG - chr4_1:85597749-85597768 Msa0580500:intron 35.0%
AAACCGCCTTCTTTATCAAA+TGG + chr4_1:85597755-85597774 None:intergenic 35.0%
AATCTTACTACTTCCAGACA+GGG + chr4_1:85597896-85597915 None:intergenic 35.0%
AAATCTTACTACTTCCAGAC+AGG + chr4_1:85597897-85597916 None:intergenic 35.0%
TTGATTCAACTACAAATGCC+AGG - chr4_1:85597955-85597974 Msa0580500:intron 35.0%
GAATTCGTTTACTTGTCTAG+GGG - chr4_1:85598096-85598115 Msa0580500:intron 35.0%
TGGAATGCCTTAAAACTTTG+GGG - chr4_1:85598162-85598181 Msa0580500:intron 35.0%
TTTATTGGCAGGGAAATATG+AGG - chr4_1:85598201-85598220 Msa0580500:intron 35.0%
AAAGACTTCAATGTCACTCT+TGG + chr4_1:85598598-85598617 None:intergenic 35.0%
GTAGGATAAAACACATACGA+AGG + chr4_1:85598627-85598646 None:intergenic 35.0%
TCAAAGTAATGGTAAAACCC+TGG + chr4_1:85598769-85598788 None:intergenic 35.0%
AATATTATGGTGTGATTGCC+TGG - chr4_1:85598981-85599000 Msa0580500:intron 35.0%
CAAAAGTCATTAGTTGCGAT+AGG + chr4_1:85599125-85599144 None:intergenic 35.0%
AGGTTTATCTAGAGACTTGA+TGG - chr4_1:85599263-85599282 Msa0580500:intron 35.0%
GGTTTATCTAGAGACTTGAT+GGG - chr4_1:85599264-85599283 Msa0580500:intron 35.0%
CTGGAATGCTAATTACAGAA+AGG + chr4_1:85599568-85599587 None:intergenic 35.0%
TTCTGTAATTAGCATTCCAG+AGG - chr4_1:85599568-85599587 Msa0580500:five_prime_UTR 35.0%
ATACCGCAACACTGAAATGT+TGG + chr4_1:85596605-85596624 None:intergenic 40.0%
ACATTTCAGTGTTGCGGTAT+AGG - chr4_1:85596605-85596624 Msa0580500:three_prime_UTR 40.0%
CATTTCAGTGTTGCGGTATA+GGG - chr4_1:85596606-85596625 Msa0580500:three_prime_UTR 40.0%
! GCTAGGAGAAGTTCTTTTGT+AGG + chr4_1:85596785-85596804 None:intergenic 40.0%
ACAAAAGAACTTCTCCTAGC+TGG - chr4_1:85596785-85596804 Msa0580500:three_prime_UTR 40.0%
ACGAAGAATAACACCGATCT+GGG + chr4_1:85596956-85596975 None:intergenic 40.0%
!! TACCATGAGCTTTGAGGATA+CGG + chr4_1:85597029-85597048 None:intergenic 40.0%
!! GCTGTTTTCACAAGTTATCG+GGG - chr4_1:85597171-85597190 Msa0580500:intron 40.0%
TTCAGCACCTCAAAATTTCG+AGG + chr4_1:85597236-85597255 None:intergenic 40.0%
!! ACCTGTTCGTAAGCTGTTTT+CGG + chr4_1:85597431-85597450 None:intergenic 40.0%
CCACATGGACACAAATTCAT+AGG + chr4_1:85597585-85597604 None:intergenic 40.0%
CCTATGAATTTGTGTCCATG+TGG - chr4_1:85597582-85597601 Msa0580500:intron 40.0%
ATCAAATGGTACAGATCCTC+GGG + chr4_1:85597741-85597760 None:intergenic 40.0%
AACAAGGATGAGTCTGAACT+TGG + chr4_1:85597820-85597839 None:intergenic 40.0%
TCTTGTAATAGCGAGCAAGA+CGG + chr4_1:85597857-85597876 None:intergenic 40.0%
ATCTTACTACTTCCAGACAG+GGG + chr4_1:85597895-85597914 None:intergenic 40.0%
! TTCGATATTGAGCTTTGTGG+TGG - chr4_1:85598019-85598038 Msa0580500:intron 40.0%
! TCTAGGTCCCCAAAGTTTTA+AGG + chr4_1:85598172-85598191 None:intergenic 40.0%
ATGGGTCCTAGTGAACTTTA+CGG + chr4_1:85598339-85598358 None:intergenic 40.0%
CTAGTCCTCTCTGAATAGAA+CGG - chr4_1:85598424-85598443 Msa0580500:intron 40.0%
GTTAACCGTTCTATTCAGAG+AGG + chr4_1:85598432-85598451 None:intergenic 40.0%
GCACAGCAACATGAATGAAA+TGG - chr4_1:85598840-85598859 Msa0580500:intron 40.0%
AATTAACAGGAAGGCAAGAC+TGG + chr4_1:85599085-85599104 None:intergenic 40.0%
CATTAGTTGCGATAGGAACA+TGG + chr4_1:85599118-85599137 None:intergenic 40.0%
TTGGTTGCTTAGAGCTTGTA+TGG - chr4_1:85599176-85599195 Msa0580500:CDS 40.0%
TGGTTGCTTAGAGCTTGTAT+GGG - chr4_1:85599177-85599196 Msa0580500:CDS 40.0%
!! CAATCTGCAAGGTCATTTTG+AGG - chr4_1:85599243-85599262 Msa0580500:intron 40.0%
!! AGAAAATTGACTTGCTCCTC+TGG + chr4_1:85599587-85599606 None:intergenic 40.0%
! CTCTTGCTTGTGTTTGGGTT+GGG + chr4_1:85596530-85596549 None:intergenic 45.0%
ACTCTTGCTTGTGTTTGGGT+TGG + chr4_1:85596531-85596550 None:intergenic 45.0%
TTTACTATGCATACGCCCCA+TGG + chr4_1:85596653-85596672 None:intergenic 45.0%
ATGGGGCGTATGCATAGTAA+AGG - chr4_1:85596652-85596671 Msa0580500:three_prime_UTR 45.0%
TGGGGCGTATGCATAGTAAA+GGG - chr4_1:85596653-85596672 Msa0580500:three_prime_UTR 45.0%
! TCTTTTGTAGGGTAAAGCGG+AGG + chr4_1:85596773-85596792 None:intergenic 45.0%
GAAGAGATCTTAAGCCAGCT+AGG + chr4_1:85596802-85596821 None:intergenic 45.0%
GCTTAAGATCTCTTCACCAG+AGG - chr4_1:85596807-85596826 Msa0580500:three_prime_UTR 45.0%
AGAATAACACCGATCTGGGA+AGG + chr4_1:85596952-85596971 None:intergenic 45.0%
CACGAAGAATAACACCGATC+TGG + chr4_1:85596957-85596976 None:intergenic 45.0%
GAGAGATACCATGAGCTTTG+AGG + chr4_1:85597035-85597054 None:intergenic 45.0%
! TTTTCACAAGTTATCGGGGC+TGG - chr4_1:85597175-85597194 Msa0580500:intron 45.0%
! CACAAGGCCTCGAAATTTTG+AGG - chr4_1:85597226-85597245 Msa0580500:intron 45.0%
GATAGCATTGCAAGTTACGC+TGG - chr4_1:85597387-85597406 Msa0580500:intron 45.0%
GGTTTCAATCCGCAAGCATT+TGG - chr4_1:85597770-85597789 Msa0580500:intron 45.0%
TGTTCCTCTCCAAATGCTTG+CGG + chr4_1:85597782-85597801 None:intergenic 45.0%
GCATTTGGAGAGGAACAGAA+AGG - chr4_1:85597785-85597804 Msa0580500:intron 45.0%
TCTTACTACTTCCAGACAGG+GGG + chr4_1:85597894-85597913 None:intergenic 45.0%
! GTTAATTCGGTGACTAAGCC+TGG + chr4_1:85597976-85597995 None:intergenic 45.0%
TGGGTCCTAGTGAACTTTAC+GGG + chr4_1:85598338-85598357 None:intergenic 45.0%
ACAAGATCCTTCCTAAGTGC+AGG + chr4_1:85598367-85598386 None:intergenic 45.0%
ATGAAGGAAGATGCACCCAA+GGG + chr4_1:85598462-85598481 None:intergenic 45.0%
GACTAGAGCTGAAGATGACA+CGG + chr4_1:85598514-85598533 None:intergenic 45.0%
GCAAGACTGGAAAGTTCAAG+AGG + chr4_1:85599072-85599091 None:intergenic 45.0%
ATCAACAACTGCCAGCACTT+TGG - chr4_1:85599157-85599176 Msa0580500:CDS 45.0%
GGCTCATTCTTCATGCTTTC+CGG - chr4_1:85599198-85599217 Msa0580500:intron 45.0%
TTCATGCTTTCCGGTAACAG+AGG - chr4_1:85599207-85599226 Msa0580500:intron 45.0%
TCATGCTTTCCGGTAACAGA+GGG - chr4_1:85599208-85599227 Msa0580500:intron 45.0%
! ATGCTTGCTGATCGCTTAGA+AGG - chr4_1:85599493-85599512 Msa0580500:five_prime_UTR 45.0%
! TGCTTGCTGATCGCTTAGAA+GGG - chr4_1:85599494-85599513 Msa0580500:five_prime_UTR 45.0%
!!! ATTAAAAATTAATTAAGTTT+TGG - chr4_1:85598142-85598161 Msa0580500:intron 5.0%
GGTTTGACTCCTTCCCAGAT+CGG - chr4_1:85596940-85596959 Msa0580500:CDS 50.0%
TGATTCTCACGGCATTGCTC+AGG - chr4_1:85596975-85596994 Msa0580500:intron 50.0%
!! CATGAGCTTTGAGGATACGG+AGG + chr4_1:85597026-85597045 None:intergenic 50.0%
CTCCGTATCCTCAAAGCTCA+TGG - chr4_1:85597024-85597043 Msa0580500:intron 50.0%
GCAAAGCGGGATACTACACA+AGG - chr4_1:85597210-85597229 Msa0580500:intron 50.0%
GCCGAAAACAGCTTACGAAC+AGG - chr4_1:85597427-85597446 Msa0580500:intron 50.0%
TACAGATCCTCGGGAATCTC+AGG + chr4_1:85597732-85597751 None:intergenic 50.0%
CAATCCGCAAGCATTTGGAG+AGG - chr4_1:85597775-85597794 Msa0580500:intron 50.0%
GGAGAGGAACAGAAAGGACA+AGG - chr4_1:85597791-85597810 Msa0580500:intron 50.0%
! GGTGGATTCTGCTCTCAACA+AGG + chr4_1:85597836-85597855 None:intergenic 50.0%
TGTAATAGCGAGCAAGACGG+TGG + chr4_1:85597854-85597873 None:intergenic 50.0%
GGCATCCCGTAAAGTTCACT+AGG - chr4_1:85598330-85598349 Msa0580500:CDS 50.0%
TCCTAAGTGCAGGTGCTCAT+GGG + chr4_1:85598357-85598376 None:intergenic 50.0%
TTCCTAAGTGCAGGTGCTCA+TGG + chr4_1:85598358-85598377 None:intergenic 50.0%
ATGAGCACCTGCACTTAGGA+AGG - chr4_1:85598357-85598376 Msa0580500:CDS 50.0%
ACGGTTAACATCCTTCCCCT+TGG - chr4_1:85598443-85598462 Msa0580500:intron 50.0%
CGGTTAACATCCTTCCCCTT+GGG - chr4_1:85598444-85598463 Msa0580500:intron 50.0%
TGAAGGAAGATGCACCCAAG+GGG + chr4_1:85598461-85598480 None:intergenic 50.0%
CATGAAGGAAGATGCACCCA+AGG + chr4_1:85598463-85598482 None:intergenic 50.0%
! TGTTGCTGTGCAGAAGCACA+CGG + chr4_1:85598831-85598850 None:intergenic 50.0%
! GCTCTAAGCAACCAAAGTGC+TGG + chr4_1:85599171-85599190 None:intergenic 50.0%
ACAGCCGAAGCCAGAAACCA+TGG - chr4_1:85596633-85596652 Msa0580500:three_prime_UTR 55.0%
CAGCCGAAGCCAGAAACCAT+GGG - chr4_1:85596634-85596653 Msa0580500:three_prime_UTR 55.0%
AGCCGAAGCCAGAAACCATG+GGG - chr4_1:85596635-85596654 Msa0580500:three_prime_UTR 55.0%
TGCATACGCCCCATGGTTTC+TGG + chr4_1:85596646-85596665 None:intergenic 55.0%
!! GCTCAGGTCAAGAGCGTTAC+TGG - chr4_1:85596991-85597010 Msa0580500:intron 55.0%
TGTAGTATCCCGCTTTGCGC+AGG + chr4_1:85597208-85597227 None:intergenic 55.0%
ACTTGCACCTGAGATTCCCG+AGG - chr4_1:85597722-85597741 Msa0580500:intron 55.0%
ACCCATGAGCACCTGCACTT+AGG - chr4_1:85598353-85598372 Msa0580500:CDS 55.0%
! GTTGCTGTGCAGAAGCACAC+GGG + chr4_1:85598830-85598849 None:intergenic 55.0%
GTGCAGCAACCCTCTGTTAC+CGG + chr4_1:85599220-85599239 None:intergenic 55.0%
GTAACAGAGGGTTGCTGCAC+TGG - chr4_1:85599220-85599239 Msa0580500:intron 55.0%
! TGCTGCACTGGCAATCTGCA+AGG - chr4_1:85599232-85599251 Msa0580500:intron 55.0%
!! CGCCCCATGGTTTCTGGCTT+CGG + chr4_1:85596640-85596659 None:intergenic 60.0%
GGTGAACAGCCTGCGCAAAG+CGG - chr4_1:85597196-85597215 Msa0580500:intron 60.0%
GTGAACAGCCTGCGCAAAGC+GGG - chr4_1:85597197-85597216 Msa0580500:intron 60.0%
CCAGACAGGGGGAAGCTTCT+TGG + chr4_1:85597883-85597902 None:intergenic 60.0%
CCAAGAAGCTTCCCCCTGTC+TGG - chr4_1:85597880-85597899 Msa0580500:intron 60.0%
GGAAGATGCACCCAAGGGGA+AGG + chr4_1:85598457-85598476 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 85596511 85599607 85596511 ID=Msa0580500;Name=Msa0580500
chr4_1 mRNA 85596511 85599607 85596511 ID=Msa0580500-mRNA-1;Parent=Msa0580500;Name=Msa0580500-mRNA-1;_AED=0.10;_eAED=0.10;_QI=141|0.75|0.8|1|0.75|0.8|5|420|127
chr4_1 exon 85596511 85596964 85596511 ID=Msa0580500-mRNA-1:exon:31500;Parent=Msa0580500-mRNA-1
chr4_1 exon 85598211 85598397 85598211 ID=Msa0580500-mRNA-1:exon:31499;Parent=Msa0580500-mRNA-1
chr4_1 exon 85599145 85599211 85599145 ID=Msa0580500-mRNA-1:exon:31498;Parent=Msa0580500-mRNA-1
chr4_1 exon 85599290 85599362 85599290 ID=Msa0580500-mRNA-1:exon:31497;Parent=Msa0580500-mRNA-1
chr4_1 exon 85599444 85599607 85599444 ID=Msa0580500-mRNA-1:exon:31496;Parent=Msa0580500-mRNA-1
chr4_1 five_prime_UTR 85599467 85599607 85599467 ID=Msa0580500-mRNA-1:five_prime_utr;Parent=Msa0580500-mRNA-1
chr4_1 CDS 85599444 85599466 85599444 ID=Msa0580500-mRNA-1:cds;Parent=Msa0580500-mRNA-1
chr4_1 CDS 85599290 85599362 85599290 ID=Msa0580500-mRNA-1:cds;Parent=Msa0580500-mRNA-1
chr4_1 CDS 85599145 85599211 85599145 ID=Msa0580500-mRNA-1:cds;Parent=Msa0580500-mRNA-1
chr4_1 CDS 85598211 85598397 85598211 ID=Msa0580500-mRNA-1:cds;Parent=Msa0580500-mRNA-1
chr4_1 CDS 85596931 85596964 85596931 ID=Msa0580500-mRNA-1:cds;Parent=Msa0580500-mRNA-1
chr4_1 three_prime_UTR 85596511 85596930 85596511 ID=Msa0580500-mRNA-1:three_prime_utr;Parent=Msa0580500-mRNA-1
Gene Sequence

>Msa0580500

ATGGGGCGTATGCATAGTAAAGGTAAGGGTATTTCTTCCTCCGCTTTACCCTACAAAAGAACTTCTCCTAGCTGGCTTAAGATCTCTTCACCAGAGGTTGATGAGACTATTTGCAAGTTTGCTAAGAAGGGTTTGACTCCTTCCCAGATCGGTGTTATTCTTCGACTTGCACCTGAGATTCCCGAGGATCTGTACCATTTGATAAAGAAGGCGGTTTCAATCCGCAAGCATTTGGAGAGGAACAGAAAGGACAAGGACTCCAAGTTCAGACTCATCCTTGTTGAGAGCAGAATCCACCGTCTTGCTCGCTATTACAAGAAAACCAAGAAGCTTCCCCCTGTCTGGAAGTACGAATCAACAACTGCCAGCACTTTGGTTGCTTAG
Protein sequence

>Msa0580500

MGRMHSKGKGISSSALPYKRTSPSWLKISSPEVDETICKFAKKGLTPSQIGVILRLAPEIPEDLYHLIKKAVSIRKHLERNRKDKDSKFRLILVESRIHRLARYYKKTKKLPPVWKYESTTASTLVA*