AlfalfaGEDB | Msa0756840

M. sativa cultivar zhongmu-4 / Msa0756840

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007275 GO:0007623 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0009639 GO:0009791 GO:0009889 GO:0009900 GO:0009908 GO:0010029 GO:0010047 GO:0010114 GO:0010154 GO:0010187 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048511 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0099402 GO:0140110 GO:1900140 GO:1903506 GO:2000026 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756840	sp\|Q9FUA4\|SPT_ARATH	49.099	222	86	5	78	296	160	357	2.90e-46	164

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756840	G7K6L5	94.925	335	16	1	14	347	10	344	0.0	604

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TFs/TRs:

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Gene ID	Type	Classification
Msa0756840	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0342990	Msa0756840	0.925739	1.219080e-90	-8.615850e-47
Msa0714770	Msa0756840	0.913517	5.614260e-84	-8.615850e-47
Msa0756840	Msa0836170	0.948388	1.041375e-106	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756840	MtrunA17_Chr5g0402031	94.925	335	16	1	14	347	10	344	0.0	604
Msa0756840	MtrunA17_Chr1g0153241	81.319	91	16	1	97	186	73	163	3.26e-42	147
Msa0756840	MtrunA17_Chr1g0193321	57.983	119	49	1	79	197	434	551	1.28e-33	131
Msa0756840	MtrunA17_Chr7g0262691	57.500	120	42	3	72	183	213	331	6.06e-30	119
Msa0756840	MtrunA17_Chr1g0182791	59.813	107	38	3	77	183	289	390	1.84e-29	118
Msa0756840	MtrunA17_Chr3g0095391	48.551	138	60	5	109	243	310	439	1.30e-26	109
Msa0756840	MtrunA17_Chr7g0271251	55.140	107	42	1	104	204	428	534	4.24e-24	103
Msa0756840	MtrunA17_Chr7g0231461	68.182	66	21	0	109	174	298	363	9.53e-23	99.0
Msa0756840	MtrunA17_Chr7g0270871	50.549	91	42	1	88	178	185	272	3.94e-22	95.9
Msa0756840	MtrunA17_Chr4g0074161	36.029	136	67	2	40	172	152	270	6.29e-19	87.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756840	AT4G36930.1	49.099	222	86	5	78	296	160	357	2.95e-47	164
Msa0756840	AT5G67110.1	52.381	168	57	5	16	181	14	160	3.62e-37	133
Msa0756840	AT1G09530.3	60.177	113	40	3	82	191	311	421	1.73e-31	124
Msa0756840	AT1G09530.2	60.177	113	40	3	82	191	311	421	1.73e-31	124
Msa0756840	AT1G09530.5	60.177	113	40	3	82	191	311	421	1.73e-31	124
Msa0756840	AT1G09530.1	60.177	113	40	3	82	191	311	421	1.73e-31	124
Msa0756840	AT1G09530.4	60.177	113	40	3	82	191	311	421	1.73e-31	124
Msa0756840	AT1G09530.6	60.177	113	40	3	82	191	311	421	1.73e-31	124
Msa0756840	AT2G20180.7	64.516	93	23	1	80	172	189	271	6.43e-30	118
Msa0756840	AT2G20180.1	64.516	93	23	1	80	172	189	271	6.43e-30	118

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Find 88 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATACAAACCTGTACT+TGG	0.469577	5_2:+11408824	None:intergenic
AACAACACAGAAACTTCTTT+CGG	0.333925	5_2:-11407974	Msa0756840:CDS
AACAAGTCTTCGCCGTGTAT+TGG	0.455886	5_2:+11407805	None:intergenic
AACATAACAACAACATGGCT+CGG	0.564736	5_2:-11409935	Msa0756840:CDS
AAGAAGATGAAGATGAACGA+AGG	0.614318	5_2:+11409853	None:intergenic
AAGCCTTCATGAAAGTACAT+AGG	0.613283	5_2:+11408178	None:intergenic
AAGGAGTTGTCTAGGGGATG+AGG	0.512056	5_2:+11408073	None:intergenic
AAGGGATTTCTATGTTTACG+AGG	0.664139	5_2:+11407393	None:intergenic
AATCTATTAGGCGGTTCTCA+AGG	0.591562	5_2:-11407745	Msa0756840:intron
AATGGAACATAACAACAACA+TGG	0.706557	5_2:-11409940	Msa0756840:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGGAACATAACAACAACA+TGG	-	chr5_2:11407414-11407433	Msa0756840:three_prime_UTR	30.0%
TTTACTCAATGTCTTGGAAA+AGG	-	chr5_2:11407845-11407864	Msa0756840:intron	30.0%
TTATGCACCTTGTTAGAATT+TGG	+	chr5_2:11408371-11408390	None:intergenic	30.0%
AAAAGATACAAACCTGTACT+TGG	+	chr5_2:11408533-11408552	None:intergenic	30.0%
ATTCTAAATACTTCTCTCGA+CGG	-	chr5_2:11408795-11408814	Msa0756840:intron	30.0%
ACGTCTTAAATTTGAATCGT+CGG	-	chr5_2:11408847-11408866	Msa0756840:CDS	30.0%
AGAGAATTACCAACATTTGA+AGG	-	chr5_2:11408915-11408934	Msa0756840:intron	30.0%
ATAAAGTAAGGAGTTGTCTA+GGG	+	chr5_2:11409291-11409310	None:intergenic	30.0%
TAGATTGGATGCATAAAGTA+AGG	+	chr5_2:11409303-11409322	None:intergenic	30.0%
AACAACACAGAAACTTCTTT+CGG	-	chr5_2:11409380-11409399	Msa0756840:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	11407326	11410050	11407326	ID=Msa0756840;Name=Msa0756840
chr5_2	mRNA	11407326	11410050	11407326	ID=Msa0756840-mRNA-1;Parent=Msa0756840;Name=Msa0756840-mRNA-1;_AED=0.03;_eAED=0.03;_QI=89\|0.83\|0.85\|1\|1\|1\|7\|188\|347
chr5_2	exon	11407326	11407659	11407326	ID=Msa0756840-mRNA-1:exon:5148;Parent=Msa0756840-mRNA-1
chr5_2	exon	11407746	11407827	11407746	ID=Msa0756840-mRNA-1:exon:5147;Parent=Msa0756840-mRNA-1
chr5_2	exon	11407922	11408233	11407922	ID=Msa0756840-mRNA-1:exon:5146;Parent=Msa0756840-mRNA-1
chr5_2	exon	11408837	11408902	11408837	ID=Msa0756840-mRNA-1:exon:5145;Parent=Msa0756840-mRNA-1
chr5_2	exon	11408994	11409059	11408994	ID=Msa0756840-mRNA-1:exon:5144;Parent=Msa0756840-mRNA-1
chr5_2	exon	11409155	11409262	11409155	ID=Msa0756840-mRNA-1:exon:5143;Parent=Msa0756840-mRNA-1
chr5_2	exon	11409698	11410050	11409698	ID=Msa0756840-mRNA-1:exon:5142;Parent=Msa0756840-mRNA-1
chr5_2	five_prime_UTR	11409962	11410050	11409962	ID=Msa0756840-mRNA-1:five_prime_utr;Parent=Msa0756840-mRNA-1
chr5_2	CDS	11409698	11409961	11409698	ID=Msa0756840-mRNA-1:cds;Parent=Msa0756840-mRNA-1
chr5_2	CDS	11409155	11409262	11409155	ID=Msa0756840-mRNA-1:cds;Parent=Msa0756840-mRNA-1
chr5_2	CDS	11408994	11409059	11408994	ID=Msa0756840-mRNA-1:cds;Parent=Msa0756840-mRNA-1
chr5_2	CDS	11408837	11408902	11408837	ID=Msa0756840-mRNA-1:cds;Parent=Msa0756840-mRNA-1
chr5_2	CDS	11407922	11408233	11407922	ID=Msa0756840-mRNA-1:cds;Parent=Msa0756840-mRNA-1
chr5_2	CDS	11407746	11407827	11407746	ID=Msa0756840-mRNA-1:cds;Parent=Msa0756840-mRNA-1
chr5_2	CDS	11407514	11407659	11407514	ID=Msa0756840-mRNA-1:cds;Parent=Msa0756840-mRNA-1
chr5_2	three_prime_UTR	11407326	11407513	11407326	ID=Msa0756840-mRNA-1:three_prime_utr;Parent=Msa0756840-mRNA-1

Gene Sequence

>Msa0756840

ATGGAACATAACAACAACATGGCTCGGTTTGACAAAAACATGTCCTCTTCACAAGACGAAATCTCTCTTTTTCTTCATCAAATTCTCCTTCGTTCATCTTCATCTTCTTCCATGCCAGGATCCATTAACCAGCTTCAAGATGGAAAGATCTCAGCCTTTGATTCAACTTCTAGACCAAACCATGCTTTAAATGTATCTTCCTCTTCTGTTGGAGCAGGAGGACTCAGTGGTAATGACACTGATGAATATGACTGTGAAAGTGAGGAGGGTGTGGAAGCATTGATTGAAGAAGTTCCAACAAAGTCAGCTGGTGCTTCTAGGAGTTCTTCAAAAAGAAGTAGAGCAGCTGAAGTTCATAATTTGTCAGAAAAGAGAAGAAGGAGTAGGATTAATGAGAAAATGAAAGCTTTACAAAATCTAATTCCAAATTCTAACAAGACTGATAAAGCTTCAATGCTAGATGAAGCTATTGAATACCTTAAACAGCTTCAGCTCCAAGTACAGATGCTGTCATTGAGAAATGGATTGAGTTTGCATCCTATGTACTTTCATGAAGGCTTACATTCTCTGCCTTTGTCTCGGATGAGTACGGAGTTGAGTGAAGGAAACGGTTCTAACCCTTTAAATATGACATCTACTCTGCCTCATCCCCTAGACAACTCCTTACTTTATGCATCCAATCTACCTAACAAAAACACATTGTCGAGTCAGCCATCAATGTCTTCCTATCCTTCATATATTAACAACACAGAAACTTCTTTCGGGGTGGAATCTGGAATCCCAGCACAGAAAAAACGTCTCCAACAATCTTCAGAGCCAATACACGGCGAAGACTTGTTGCAGCACCAGCAATTGAATGCAATAAATTCTTTTACCAATCTATTAGGCGGTTCTCAAGCTGTCAAAGAATTTCAGTCGGGCGCAATGGCTACACCTACTACTAACAATACTTTGCAAACTTGTGTTGCCGGAAGAGATCAGTCAGGAGCGGTCATGAGAAATAGTGAGCCCAACATTATATTTACATCGCAGTTAAGTAGGTAA

Protein sequence

>Msa0756840

MEHNNNMARFDKNMSSSQDEISLFLHQILLRSSSSSSMPGSINQLQDGKISAFDSTSRPNHALNVSSSSVGAGGLSGNDTDEYDCESEEGVEALIEEVPTKSAGASRSSSKRSRAAEVHNLSEKRRRSRINEKMKALQNLIPNSNKTDKASMLDEAIEYLKQLQLQVQMLSLRNGLSLHPMYFHEGLHSLPLSRMSTELSEGNGSNPLNMTSTLPHPLDNSLLYASNLPNKNTLSSQPSMSSYPSYINNTETSFGVESGIPAQKKRLQQSSEPIHGEDLLQHQQLNAINSFTNLLGGSQAVKEFQSGAMATPTTNNTLQTCVAGRDQSGAVMRNSEPNIIFTSQLSR*