Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0757750 | sp|Q8W4F0|DAR1_ARATH | 74.545 | 55 | 7 | 2 | 1 | 48 | 342 | 396 | 8.27e-17 | 75.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0757750 | K7LNC3 | 71.429 | 63 | 15 | 1 | 1 | 60 | 345 | 407 | 2.74e-17 | 84.0 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0757740 | Msa0757750 | 0.842285 | 2.838761e-58 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0757750 | MtrunA17_Chr8g0373781 | 75.510 | 49 | 8 | 2 | 1 | 45 | 313 | 361 | 3.01e-16 | 70.9 |
| Msa0757750 | MtrunA17_Chr5g0436051 | 61.818 | 55 | 14 | 2 | 1 | 48 | 407 | 461 | 3.75e-15 | 67.8 |
| Msa0757750 | MtrunA17_Chr3g0112601 | 60.417 | 48 | 16 | 1 | 1 | 45 | 297 | 344 | 4.32e-14 | 64.7 |
| Msa0757750 | MtrunA17_Chr1g0154501 | 61.224 | 49 | 16 | 1 | 1 | 46 | 263 | 311 | 1.86e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0757750 | AT4G36860.3 | 74.545 | 55 | 7 | 2 | 1 | 48 | 366 | 420 | 8.03e-18 | 75.9 |
| Msa0757750 | AT4G36860.2 | 74.545 | 55 | 7 | 2 | 1 | 48 | 342 | 396 | 8.33e-18 | 75.5 |
| Msa0757750 | AT4G36860.1 | 74.545 | 55 | 7 | 2 | 1 | 48 | 342 | 396 | 8.41e-18 | 75.5 |
| Msa0757750 | AT1G19270.1 | 71.429 | 49 | 11 | 1 | 1 | 46 | 324 | 372 | 1.94e-17 | 74.7 |
| Msa0757750 | AT1G19270.2 | 71.429 | 49 | 11 | 1 | 1 | 46 | 324 | 372 | 1.94e-17 | 74.7 |
| Msa0757750 | AT2G39830.2 | 62.500 | 48 | 15 | 1 | 1 | 45 | 314 | 361 | 1.83e-15 | 68.9 |
| Msa0757750 | AT2G39830.1 | 62.500 | 48 | 15 | 1 | 1 | 45 | 314 | 361 | 2.12e-15 | 68.9 |
| Msa0757750 | AT2G39830.3 | 62.500 | 48 | 15 | 1 | 1 | 45 | 305 | 352 | 2.40e-15 | 68.6 |
| Msa0757750 | AT2G39830.4 | 62.500 | 48 | 15 | 1 | 1 | 45 | 314 | 361 | 2.92e-15 | 68.6 |
Find 21 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGCAAGTTCCTATGCTTT+TGG | 0.302344 | 5_2:+12706565 | Msa0757750:CDS |
| AAGCAGAATCCTCTAGTTTC+AGG | 0.379897 | 5_2:-12706741 | None:intergenic |
| AGTGTGTAAAGACTCGAATT+TGG | 0.401580 | 5_2:-12706806 | None:intergenic |
| TCAAGGACTTTACCTGTTGA+TGG | 0.405715 | 5_2:+12707088 | Msa0757750:CDS |
| ATCCTCTAGTTTCAGGTAAA+CGG | 0.410172 | 5_2:-12706734 | None:intergenic |
| GTTCTGTACCTCCCATCAAC+AGG | 0.480493 | 5_2:-12707100 | None:intergenic |
| ATAGGTTGTTAACAGGATCA+AGG | 0.497707 | 5_2:+12707071 | Msa0757750:intron |
| TTGGAAGCAGCCAATCCTTA+CGG | 0.503948 | 5_2:-12706787 | None:intergenic |
| ACTGTAACCACCGTAAGGAT+TGG | 0.535045 | 5_2:+12706777 | Msa0757750:CDS |
| CAAGGACTTTACCTGTTGAT+GGG | 0.548839 | 5_2:+12707089 | Msa0757750:CDS |
| ACAAGCACTAAATGAGGCCA+TGG | 0.572592 | 5_2:+12706595 | Msa0757750:CDS |
| TGAAGGTTTAAATATGAAAG+CGG | 0.579739 | 5_2:+12706541 | None:intergenic |
| AACAAACTGTAACCACCGTA+AGG | 0.585176 | 5_2:+12706772 | Msa0757750:CDS |
| CGAGAGACAAGCACTAAATG+AGG | 0.612239 | 5_2:+12706589 | Msa0757750:CDS |
| CACCGTTTACCTGAAACTAG+AGG | 0.629415 | 5_2:+12706732 | Msa0757750:CDS |
| TGTCTCTCGACCAAAAGCAT+AGG | 0.641531 | 5_2:-12706575 | None:intergenic |
| TTTGACATATACATCCTCCA+TGG | 0.643441 | 5_2:-12706612 | None:intergenic |
| GGACTTTACCTGTTGATGGG+AGG | 0.645756 | 5_2:+12707092 | Msa0757750:CDS |
| GAAGCAGCCAATCCTTACGG+TGG | 0.653785 | 5_2:-12706784 | None:intergenic |
| GAGGTACAGAACTAATTACA+AGG | 0.671044 | 5_2:+12707111 | Msa0757750:CDS |
| AGCACTAAATGAGGCCATGG+AGG | 0.685154 | 5_2:+12706598 | Msa0757750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAAGAAAACCAAGTAT+AGG | + | chr5_2:12706892-12706911 | Msa0757750:intron | 20.0% |
| !!! | TTTTAAGAAAACCAAGTATA+GGG | + | chr5_2:12706893-12706912 | Msa0757750:intron | 20.0% |
| !! | TTCAAGAACTTTATGTTTAT+AGG | + | chr5_2:12707053-12707072 | Msa0757750:intron | 20.0% |
| !!! | TACTTGGTTTTCTTAAAATC+TGG | - | chr5_2:12706891-12706910 | None:intergenic | 25.0% |
| ! | TTGTGAAAGTAGATGTTATA+TGG | - | chr5_2:12706999-12707018 | None:intergenic | 25.0% |
| ! | TATGTTTATAGGTTGTTAAC+AGG | + | chr5_2:12707064-12707083 | Msa0757750:intron | 25.0% |
| TTTGCTTTACTATCGAATGT+AGG | + | chr5_2:12706704-12706723 | Msa0757750:intron | 30.0% | |
| TTGCTTTACTATCGAATGTA+GGG | + | chr5_2:12706705-12706724 | Msa0757750:intron | 30.0% | |
| TAGATGTTATATGGTAACCT+AGG | - | chr5_2:12706990-12707009 | None:intergenic | 30.0% | |
| TTTGACATATACATCCTCCA+TGG | - | chr5_2:12706615-12706634 | None:intergenic | 35.0% | |
| AGTTAGGATAACAGAAAGAC+AGG | - | chr5_2:12706672-12706691 | None:intergenic | 35.0% | |
| CAAAGTAGCAACTCTAAGTT+AGG | - | chr5_2:12706688-12706707 | None:intergenic | 35.0% | |
| ! | ATCCTCTAGTTTCAGGTAAA+CGG | - | chr5_2:12706737-12706756 | None:intergenic | 35.0% |
| AGTGTGTAAAGACTCGAATT+TGG | - | chr5_2:12706809-12706828 | None:intergenic | 35.0% | |
| !!! | GTGAAGTGACTTGTTTTGTA+TGG | + | chr5_2:12706960-12706979 | Msa0757750:intron | 35.0% |
| TGTTATATGGTAACCTAGGA+AGG | - | chr5_2:12706986-12707005 | None:intergenic | 35.0% | |
| ATAGGTTGTTAACAGGATCA+AGG | + | chr5_2:12707071-12707090 | Msa0757750:intron | 35.0% | |
| AGGATAACAGAAAGACAGGA+GGG | - | chr5_2:12706668-12706687 | None:intergenic | 40.0% | |
| TAGGATAACAGAAAGACAGG+AGG | - | chr5_2:12706669-12706688 | None:intergenic | 40.0% | |
| ! | AAGCAGAATCCTCTAGTTTC+AGG | - | chr5_2:12706744-12706763 | None:intergenic | 40.0% |
| AACAAACTGTAACCACCGTA+AGG | + | chr5_2:12706772-12706791 | Msa0757750:CDS | 40.0% | |
| AACAAGTCACTTCACATCCA+CGG | - | chr5_2:12706955-12706974 | None:intergenic | 40.0% | |
| !! | TTGTTTTGTATGGCCTTCCT+AGG | + | chr5_2:12706970-12706989 | Msa0757750:intron | 40.0% |
| TCAAGGACTTTACCTGTTGA+TGG | + | chr5_2:12707088-12707107 | Msa0757750:CDS | 40.0% | |
| CAAGGACTTTACCTGTTGAT+GGG | + | chr5_2:12707089-12707108 | Msa0757750:CDS | 40.0% | |
| ! | GCAGCAAGTTCCTATGCTTT+TGG | + | chr5_2:12706565-12706584 | Msa0757750:CDS | 45.0% |
| ! | TGTCTCTCGACCAAAAGCAT+AGG | - | chr5_2:12706578-12706597 | None:intergenic | 45.0% |
| CGAGAGACAAGCACTAAATG+AGG | + | chr5_2:12706589-12706608 | Msa0757750:CDS | 45.0% | |
| ACAAGCACTAAATGAGGCCA+TGG | + | chr5_2:12706595-12706614 | Msa0757750:CDS | 45.0% | |
| TAACAGAAAGACAGGAGGGA+AGG | - | chr5_2:12706664-12706683 | None:intergenic | 45.0% | |
| CACCGTTTACCTGAAACTAG+AGG | + | chr5_2:12706732-12706751 | Msa0757750:CDS | 45.0% | |
| ACTGTAACCACCGTAAGGAT+TGG | + | chr5_2:12706777-12706796 | Msa0757750:CDS | 45.0% | |
| TTGGAAGCAGCCAATCCTTA+CGG | - | chr5_2:12706790-12706809 | None:intergenic | 45.0% | |
| ACAAGTCACTTCACATCCAC+GGG | - | chr5_2:12706954-12706973 | None:intergenic | 45.0% | |
| AGCACTAAATGAGGCCATGG+AGG | + | chr5_2:12706598-12706617 | Msa0757750:CDS | 50.0% | |
| TGTCTGCGACTCCCTATACT+TGG | - | chr5_2:12706907-12706926 | None:intergenic | 50.0% | |
| AGTATAGGGAGTCGCAGACA+TGG | + | chr5_2:12706907-12706926 | Msa0757750:intron | 50.0% | |
| ACGGGTCAGTCTATAAGGCT+TGG | - | chr5_2:12706936-12706955 | None:intergenic | 50.0% | |
| ! | AAGCCTTATAGACTGACCCG+TGG | + | chr5_2:12706935-12706954 | Msa0757750:intron | 50.0% |
| CATCCACGGGTCAGTCTATA+AGG | - | chr5_2:12706941-12706960 | None:intergenic | 50.0% | |
| ! | GGACTTTACCTGTTGATGGG+AGG | + | chr5_2:12707092-12707111 | Msa0757750:CDS | 50.0% |
| GTTCTGTACCTCCCATCAAC+AGG | - | chr5_2:12707103-12707122 | None:intergenic | 50.0% | |
| GAAGCAGCCAATCCTTACGG+TGG | - | chr5_2:12706787-12706806 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 12706554 | 12707130 | 12706554 | ID=Msa0757750;Name=Msa0757750 |
| chr5_2 | mRNA | 12706554 | 12707130 | 12706554 | ID=Msa0757750-mRNA-1;Parent=Msa0757750;Name=Msa0757750-mRNA-1;_AED=0.56;_eAED=0.58;_QI=0|0|0|0.66|0.5|0.66|3|0|68 |
| chr5_2 | exon | 12706554 | 12706622 | 12706554 | ID=Msa0757750-mRNA-1:exon:5718;Parent=Msa0757750-mRNA-1 |
| chr5_2 | exon | 12706726 | 12706815 | 12706726 | ID=Msa0757750-mRNA-1:exon:5719;Parent=Msa0757750-mRNA-1 |
| chr5_2 | exon | 12707086 | 12707130 | 12707086 | ID=Msa0757750-mRNA-1:exon:5720;Parent=Msa0757750-mRNA-1 |
| chr5_2 | CDS | 12706554 | 12706622 | 12706554 | ID=Msa0757750-mRNA-1:cds;Parent=Msa0757750-mRNA-1 |
| chr5_2 | CDS | 12706726 | 12706815 | 12706726 | ID=Msa0757750-mRNA-1:cds;Parent=Msa0757750-mRNA-1 |
| chr5_2 | CDS | 12707086 | 12707130 | 12707086 | ID=Msa0757750-mRNA-1:cds;Parent=Msa0757750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
