AlfalfaGEDB | Msa0760850

M. sativa cultivar zhongmu-4 / Msa0760850

KEGG: K09510
GO: GO:0000226 GO:0001578 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005929 GO:0005930 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0030030 GO:0030031 GO:0031514 GO:0032838 GO:0035082 GO:0036126 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044782 GO:0060271 GO:0070925 GO:0071840 GO:0097014 GO:0097223 GO:0097224 GO:0097729 GO:0099568 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1904158
PFAM: PF00226.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760850	XP_003593643.1	100.000	54	0	0	1	54	1	54	1.01e-28	115

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760850	sp\|A9IGC5\|DNAJ_BORPD	69.118	68	17	2	4	71	5	68	7.61e-21	86.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760850	A2Q503	100.000	54	0	0	1	54	1	54	4.82e-29	115

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0760850	Msa0763120	0.917803	3.399636e-86	-8.615850e-47
Msa0243100	Msa0760850	0.808727	2.686495e-50	-8.615850e-47
Msa0484780	Msa0760850	0.806120	9.600065e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760850	MtrunA17_Chr2g0282761	100.000	54	0	0	1	54	1	54	9.28e-33	115
Msa0760850	MtrunA17_Chr4g0040501	85.185	54	8	0	1	54	1	54	6.79e-27	99.8
Msa0760850	MtrunA17_Chr2g0282741	83.333	54	9	0	1	54	1	54	3.33e-26	97.8
Msa0760850	MtrunA17_Chr4g0006391	77.778	54	12	0	1	54	1	54	5.50e-26	97.1
Msa0760850	MtrunA17_Chr3g0138471	77.193	57	13	0	1	57	1	57	7.17e-26	96.7
Msa0760850	MtrunA17_Chr2g0324921	77.778	54	12	0	1	54	1	54	6.83e-25	94.4
Msa0760850	MtrunA17_Chr7g0263971	56.863	51	22	0	4	54	369	419	8.09e-16	70.1
Msa0760850	MtrunA17_Chr7g0269841	62.000	50	18	1	5	54	27	75	7.39e-15	67.0
Msa0760850	MtrunA17_Chr3g0093111	50.943	53	25	1	3	54	1	53	3.80e-12	59.3
Msa0760850	MtrunA17_Chr5g0415731	46.053	76	31	2	4	72	5	77	4.51e-12	57.4
Msa0760850	MtrunA17_Chr7g0271891	44.643	56	28	1	5	57	17	72	6.28e-12	57.4
Msa0760850	MtrunA17_Chr5g0427221	51.852	54	25	1	4	57	48	100	7.20e-12	56.6
Msa0760850	MtrunA17_Chr3g0123861	58.824	51	19	1	4	54	82	130	1.42e-11	57.8
Msa0760850	MtrunA17_Chr4g0021141	49.020	51	25	1	4	54	22	71	1.53e-11	55.8
Msa0760850	MtrunA17_Chr5g0426951	45.588	68	33	2	4	71	83	146	2.68e-11	57.0
Msa0760850	MtrunA17_Chr6g0460461	39.683	63	34	1	3	61	1	63	3.74e-11	55.8
Msa0760850	MtrunA17_Chr3g0091361	46.429	56	30	0	3	58	4	59	4.43e-11	55.8
Msa0760850	MtrunA17_Chr6g0460411	39.683	63	34	1	3	61	1	63	5.63e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760850	AT5G01390.3	77.778	54	12	0	1	54	1	54	4.15e-26	95.9
Msa0760850	AT3G08910.1	79.630	54	11	0	1	54	1	54	4.50e-26	97.4
Msa0760850	AT5G01390.4	77.778	54	12	0	1	54	1	54	4.85e-26	95.5
Msa0760850	AT5G01390.2	77.778	54	12	0	1	54	1	54	1.04e-25	95.9
Msa0760850	AT5G01390.1	77.778	54	12	0	1	54	1	54	1.87e-25	95.9
Msa0760850	AT4G28480.2	77.778	54	12	0	1	54	1	54	2.34e-25	95.1
Msa0760850	AT4G28480.1	77.778	54	12	0	1	54	1	54	4.41e-25	95.1
Msa0760850	AT2G20560.1	75.926	54	13	0	1	54	1	54	1.32e-24	94.0
Msa0760850	AT5G25530.2	75.926	54	13	0	1	54	1	54	2.39e-24	93.2
Msa0760850	AT5G25530.1	75.926	54	13	0	1	54	1	54	2.51e-24	93.2
Msa0760850	AT1G10350.1	66.667	54	18	0	1	54	1	54	1.39e-21	85.9
Msa0760850	AT1G59725.1	67.273	55	17	1	1	54	1	55	5.76e-20	81.3
Msa0760850	AT3G47940.1	69.091	55	16	1	1	54	1	55	1.11e-19	80.9
Msa0760850	AT2G41000.2	46.429	56	26	1	3	54	2	57	2.37e-13	62.0
Msa0760850	AT5G03160.1	52.941	51	24	0	4	54	370	420	2.62e-13	63.2
Msa0760850	AT2G41000.1	46.429	56	26	1	3	54	2	57	4.08e-13	61.2
Msa0760850	AT3G62600.1	52.830	53	24	1	2	54	24	75	3.82e-12	59.7
Msa0760850	AT2G22360.1	60.784	51	18	1	4	54	86	134	4.50e-12	59.7
Msa0760850	AT5G48030.1	56.863	51	21	1	4	54	94	143	5.76e-12	59.3
Msa0760850	AT1G56300.2	49.057	53	24	1	5	54	14	66	6.64e-12	56.6
Msa0760850	AT1G56300.1	49.057	53	24	1	5	54	14	66	8.08e-12	57.0
Msa0760850	AT4G39960.1	58.824	51	19	1	4	54	85	133	1.05e-11	58.5
Msa0760850	AT4G39960.2	58.824	51	19	1	4	54	85	133	1.05e-11	58.5
Msa0760850	AT5G16650.1	52.727	55	25	1	4	58	11	64	1.53e-11	55.8
Msa0760850	AT5G16650.2	52.727	55	25	1	4	58	11	64	2.01e-11	56.2
Msa0760850	AT3G17830.1	50.000	72	27	3	2	71	61	125	3.02e-11	57.4
Msa0760850	AT1G59980.2	51.020	49	23	1	6	54	25	72	3.82e-11	57.0
Msa0760850	AT1G59980.1	51.020	49	23	1	6	54	25	72	8.08e-11	56.2

Find 30 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGGATTACTATGAGATTT+TGG	0.205034	5_2:+17499626	Msa0760850:CDS
GTAGATTTGTTGTGCGTAAT+TGG	0.247560	5_2:+17498815	Msa0760850:five_prime_UTR
TTATTGTCAGGATTCTTATC+AGG	0.253732	5_2:-17499717	None:intergenic
ACAGATTAAACCTTTATTCT+TGG	0.256184	5_2:-17498788	None:intergenic
TGATCTCCGGCCGTGGTATA+TGG	0.302314	5_2:+17500229	Msa0760850:CDS
CTTATCAGGGTGCCATTTCA+TGG	0.338061	5_2:-17499703	None:intergenic
GACTCCATGACCAAGAATAA+AGG	0.373010	5_2:+17498778	Msa0760850:five_prime_UTR
TTGGTCCAGGGTTTGATCTC+CGG	0.395380	5_2:+17500216	Msa0760850:intron
ATAGAAAACTTGCCATGAAA+TGG	0.412382	5_2:+17499691	Msa0760850:CDS
TGGTTCTAGCCTGGTGGATA+AGG	0.413461	5_2:+17498945	Msa0760850:five_prime_UTR
TCAGCATCATTCTTATTGTC+AGG	0.435149	5_2:-17499729	None:intergenic
TAAACCTTTATTCTTGGTCA+TGG	0.437062	5_2:-17498782	None:intergenic
GAAAGCTAAGTAGTGGAAGA+TGG	0.444821	5_2:+17499602	Msa0760850:exon
TATTGTCAGGATTCTTATCA+GGG	0.462954	5_2:-17499716	None:intergenic
TAAGTAGTGGAAGATGGGTT+TGG	0.464260	5_2:+17499608	Msa0760850:exon
GTAACTAAGATCAATTTAAG+AGG	0.474293	5_2:-17500277	None:intergenic
AAAGCTAAGTAGTGGAAGAT+GGG	0.475347	5_2:+17499603	Msa0760850:exon
TACATATACCACATATGCTT+CGG	0.486898	5_2:-17499772	None:intergenic
GAAAAGTGAAAGCTAAGTAG+TGG	0.495138	5_2:+17499595	Msa0760850:five_prime_UTR
GGTGGATAAGGCAAACATAC+AGG	0.505654	5_2:+17498957	Msa0760850:five_prime_UTR
CAGGGTTTGATCTCCGGCCG+TGG	0.523748	5_2:+17500222	Msa0760850:intron
ACTCATATCCGAAGCATATG+TGG	0.527454	5_2:+17499764	Msa0760850:CDS
GATTTGTTGTGCGTAATTGG+AGG	0.553928	5_2:+17498818	Msa0760850:five_prime_UTR
GTATGTTTGCCTTATCCACC+AGG	0.559232	5_2:-17498954	None:intergenic
AAAGAAAGTCCTATCTTGAG+TGG	0.579591	5_2:+17499568	Msa0760850:five_prime_UTR
TATCCACCAGGCTAGAACCA+TGG	0.582402	5_2:-17498942	None:intergenic
AATGTGTGGACTTTCTCTGA+TGG	0.591588	5_2:+17498750	Msa0760850:five_prime_UTR
TTTCCATGGTTCTAGCCTGG+TGG	0.591889	5_2:+17498939	Msa0760850:five_prime_UTR
AGAGTGTCTACAAACAGAGC+AGG	0.623091	5_2:-17498857	None:intergenic
GACTGAAATGTATTAATGTG+TGG	0.658305	5_2:+17498736	Msa0760850:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTCTGATTACTATTTATA+TGG	+	chr5_2:17499138-17499157	Msa0760850:intron	15.0%
!!	AATAATAGATAAAATATCTG+AGG	-	chr5_2:17499171-17499190	None:intergenic	15.0%
!!!	TTTTCTGATTACTATTTATA+TGG	+	chr5_2:17499138-17499157	Msa0760850:intron	15.0%
!!	AATAATAGATAAAATATCTG+AGG	-	chr5_2:17499171-17499190	None:intergenic	15.0%
!!	AAAAATAACCATCATGTAAT+GGG	-	chr5_2:17499011-17499030	None:intergenic	20.0%
!!	TAAAAATAACCATCATGTAA+TGG	-	chr5_2:17499012-17499031	None:intergenic	20.0%
!!	TGCAAAATTAACATTTAAGT+TGG	+	chr5_2:17499106-17499125	Msa0760850:intron	20.0%
!!!	CTATTATTGTGTTGTTTTAA+CGG	+	chr5_2:17499183-17499202	Msa0760850:intron	20.0%
!!	TTTATCTGTTACAACAAAAT+TGG	+	chr5_2:17499246-17499265	Msa0760850:intron	20.0%
!!	TGTAATGATTATTATTGAAC+AGG	-	chr5_2:17499517-17499536	None:intergenic	20.0%
!!!	AAATTGAAAATTGAGGATTT+TGG	-	chr5_2:17500082-17500101	None:intergenic	20.0%
!!	GGAAAAAAAATTGAAAATTG+AGG	-	chr5_2:17500089-17500108	None:intergenic	20.0%
!!	AAAAATAACCATCATGTAAT+GGG	-	chr5_2:17499011-17499030	None:intergenic	20.0%
!!	TAAAAATAACCATCATGTAA+TGG	-	chr5_2:17499012-17499031	None:intergenic	20.0%
!!	TGCAAAATTAACATTTAAGT+TGG	+	chr5_2:17499106-17499125	Msa0760850:intron	20.0%
!!!	CTATTATTGTGTTGTTTTAA+CGG	+	chr5_2:17499183-17499202	Msa0760850:intron	20.0%
!!	TTTATCTGTTACAACAAAAT+TGG	+	chr5_2:17499246-17499265	Msa0760850:intron	20.0%
!!	TGTAATGATTATTATTGAAC+AGG	-	chr5_2:17499517-17499536	None:intergenic	20.0%
!!!	AAATTGAAAATTGAGGATTT+TGG	-	chr5_2:17500082-17500101	None:intergenic	20.0%
!!	GGAAAAAAAATTGAAAATTG+AGG	-	chr5_2:17500089-17500108	None:intergenic	20.0%
!	ACAGATTAAACCTTTATTCT+TGG	-	chr5_2:17498791-17498810	None:intergenic	25.0%
!	AATGTGGTATACTTAAAGTT+TGG	-	chr5_2:17499076-17499095	None:intergenic	25.0%
!	ATTCTCACTATACTTTAACA+AGG	+	chr5_2:17499209-17499228	Msa0760850:intron	25.0%
!	ATTATTATTGAACAGGACTA+TGG	-	chr5_2:17499510-17499529	None:intergenic	25.0%
!!	TTTGGATTACTATGAGATTT+TGG	+	chr5_2:17499626-17499645	Msa0760850:CDS	25.0%
!	GTTTAATTGCTACAAAACTA+TGG	+	chr5_2:17499817-17499836	Msa0760850:intron	25.0%
!!!	ATTATCTTGTTTCTCTGTTT+TGG	+	chr5_2:17499887-17499906	Msa0760850:intron	25.0%
!!!	AAAATTGAGGATTTTGGTAA+GGG	-	chr5_2:17500076-17500095	None:intergenic	25.0%
!!	ATATGTTTTTCCATATACCA+CGG	-	chr5_2:17500242-17500261	None:intergenic	25.0%
!	GGAAAAACATATCTTATGAT+AGG	+	chr5_2:17500250-17500269	Msa0760850:CDS	25.0%
!	ACAGATTAAACCTTTATTCT+TGG	-	chr5_2:17498791-17498810	None:intergenic	25.0%
!	AATGTGGTATACTTAAAGTT+TGG	-	chr5_2:17499076-17499095	None:intergenic	25.0%
!	ATTCTCACTATACTTTAACA+AGG	+	chr5_2:17499209-17499228	Msa0760850:intron	25.0%
!	ATTATTATTGAACAGGACTA+TGG	-	chr5_2:17499510-17499529	None:intergenic	25.0%
!!	TTTGGATTACTATGAGATTT+TGG	+	chr5_2:17499626-17499645	Msa0760850:CDS	25.0%
!	GTTTAATTGCTACAAAACTA+TGG	+	chr5_2:17499817-17499836	Msa0760850:intron	25.0%
!!!	ATTATCTTGTTTCTCTGTTT+TGG	+	chr5_2:17499887-17499906	Msa0760850:intron	25.0%
!!!	AAAATTGAGGATTTTGGTAA+GGG	-	chr5_2:17500076-17500095	None:intergenic	25.0%
!!	ATATGTTTTTCCATATACCA+CGG	-	chr5_2:17500242-17500261	None:intergenic	25.0%
!	GGAAAAACATATCTTATGAT+AGG	+	chr5_2:17500250-17500269	Msa0760850:CDS	25.0%
	GACTGAAATGTATTAATGTG+TGG	+	chr5_2:17498736-17498755	Msa0760850:five_prime_UTR	30.0%
	TAAACCTTTATTCTTGGTCA+TGG	-	chr5_2:17498785-17498804	None:intergenic	30.0%
	TTACAGTTTCAAAAAAGGTC+GGG	-	chr5_2:17498892-17498911	None:intergenic	30.0%
	GTTACAGTTTCAAAAAAGGT+CGG	-	chr5_2:17498893-17498912	None:intergenic	30.0%
	CATCGTTACAGTTTCAAAAA+AGG	-	chr5_2:17498897-17498916	None:intergenic	30.0%
!	TGTTGCAAAAAGATTTTCCA+TGG	+	chr5_2:17498925-17498944	Msa0760850:five_prime_UTR	30.0%
	TTAAAGTTTGGCCTAATTAC+TGG	-	chr5_2:17499064-17499083	None:intergenic	30.0%
	TTTGCACTATGAAAAGAATG+TGG	-	chr5_2:17499092-17499111	None:intergenic	30.0%
	TTCAAAATTCCTGCTAGATA+GGG	+	chr5_2:17499274-17499293	Msa0760850:intron	30.0%
!	TGATGCTGAAATTTTCTGTT+GGG	+	chr5_2:17499375-17499394	Msa0760850:intron	30.0%
	ATAGAAAACTTGCCATGAAA+TGG	+	chr5_2:17499691-17499710	Msa0760850:CDS	30.0%
	TATTGTCAGGATTCTTATCA+GGG	-	chr5_2:17499719-17499738	None:intergenic	30.0%
	TTATTGTCAGGATTCTTATC+AGG	-	chr5_2:17499720-17499739	None:intergenic	30.0%
	TACATATACCACATATGCTT+CGG	-	chr5_2:17499775-17499794	None:intergenic	30.0%
!!	TGTTTACTGTCTTTTGTTTG+AGG	+	chr5_2:17499933-17499952	Msa0760850:intron	30.0%
	TACAATCAGTGATATACAGT+CGG	+	chr5_2:17499991-17500010	Msa0760850:intron	30.0%
	AAATTCTTTGACCCTTCTTA+GGG	-	chr5_2:17500033-17500052	None:intergenic	30.0%
	AAAATTCTTTGACCCTTCTT+AGG	-	chr5_2:17500034-17500053	None:intergenic	30.0%
!!	GAAAATTGAGGATTTTGGTA+AGG	-	chr5_2:17500077-17500096	None:intergenic	30.0%
	ACATTCAGATTGGATAATGA+AGG	-	chr5_2:17500110-17500129	None:intergenic	30.0%
	TTCATTATCCAATCTGAATG+TGG	+	chr5_2:17500109-17500128	Msa0760850:intron	30.0%
!	TTTGACTTTCATCCTAAGTA+AGG	+	chr5_2:17500175-17500194	Msa0760850:intron	30.0%
	GACTGAAATGTATTAATGTG+TGG	+	chr5_2:17498736-17498755	Msa0760850:five_prime_UTR	30.0%
	TAAACCTTTATTCTTGGTCA+TGG	-	chr5_2:17498785-17498804	None:intergenic	30.0%
	TTACAGTTTCAAAAAAGGTC+GGG	-	chr5_2:17498892-17498911	None:intergenic	30.0%
	GTTACAGTTTCAAAAAAGGT+CGG	-	chr5_2:17498893-17498912	None:intergenic	30.0%
	CATCGTTACAGTTTCAAAAA+AGG	-	chr5_2:17498897-17498916	None:intergenic	30.0%
!	TGTTGCAAAAAGATTTTCCA+TGG	+	chr5_2:17498925-17498944	Msa0760850:five_prime_UTR	30.0%
	TTAAAGTTTGGCCTAATTAC+TGG	-	chr5_2:17499064-17499083	None:intergenic	30.0%
	TTTGCACTATGAAAAGAATG+TGG	-	chr5_2:17499092-17499111	None:intergenic	30.0%
	TTCAAAATTCCTGCTAGATA+GGG	+	chr5_2:17499274-17499293	Msa0760850:intron	30.0%
!	TGATGCTGAAATTTTCTGTT+GGG	+	chr5_2:17499375-17499394	Msa0760850:intron	30.0%
	ATAGAAAACTTGCCATGAAA+TGG	+	chr5_2:17499691-17499710	Msa0760850:CDS	30.0%
	TATTGTCAGGATTCTTATCA+GGG	-	chr5_2:17499719-17499738	None:intergenic	30.0%
	TTATTGTCAGGATTCTTATC+AGG	-	chr5_2:17499720-17499739	None:intergenic	30.0%
	TACATATACCACATATGCTT+CGG	-	chr5_2:17499775-17499794	None:intergenic	30.0%
!!	TGTTTACTGTCTTTTGTTTG+AGG	+	chr5_2:17499933-17499952	Msa0760850:intron	30.0%
	TACAATCAGTGATATACAGT+CGG	+	chr5_2:17499991-17500010	Msa0760850:intron	30.0%
	AAATTCTTTGACCCTTCTTA+GGG	-	chr5_2:17500033-17500052	None:intergenic	30.0%
	AAAATTCTTTGACCCTTCTT+AGG	-	chr5_2:17500034-17500053	None:intergenic	30.0%
!!	GAAAATTGAGGATTTTGGTA+AGG	-	chr5_2:17500077-17500096	None:intergenic	30.0%
	ACATTCAGATTGGATAATGA+AGG	-	chr5_2:17500110-17500129	None:intergenic	30.0%
	TTCATTATCCAATCTGAATG+TGG	+	chr5_2:17500109-17500128	Msa0760850:intron	30.0%
!	TTTGACTTTCATCCTAAGTA+AGG	+	chr5_2:17500175-17500194	Msa0760850:intron	30.0%
!!	GTAGATTTGTTGTGCGTAAT+TGG	+	chr5_2:17498815-17498834	Msa0760850:five_prime_UTR	35.0%
!	AATGGGTTTGATCAAAACAG+AGG	-	chr5_2:17498994-17499013	None:intergenic	35.0%
	TGATCAAACCCATTACATGA+TGG	+	chr5_2:17499000-17499019	Msa0760850:intron	35.0%
	CATTGTCAGTTCCAGTAATT+AGG	+	chr5_2:17499050-17499069	Msa0760850:intron	35.0%
	CTGTTACAACAAAATTGGAG+AGG	+	chr5_2:17499251-17499270	Msa0760850:intron	35.0%
	GTTCAAAATTCCTGCTAGAT+AGG	+	chr5_2:17499273-17499292	Msa0760850:intron	35.0%
	ACATATTTACCCTATCTAGC+AGG	-	chr5_2:17499286-17499305	None:intergenic	35.0%
!	CTGATGCTGAAATTTTCTGT+TGG	+	chr5_2:17499374-17499393	Msa0760850:intron	35.0%
!	GATGCTGAAATTTTCTGTTG+GGG	+	chr5_2:17499376-17499395	Msa0760850:intron	35.0%
	GTTGATCAGAAACTTCACTT+TGG	-	chr5_2:17499473-17499492	None:intergenic	35.0%
!!!	CAGGTTTTTTGTCTTGTTTC+TGG	-	chr5_2:17499544-17499563	None:intergenic	35.0%
!	GATAGGACTTTCTTTTCTTC+AGG	-	chr5_2:17499563-17499582	None:intergenic	35.0%
	AAAGAAAGTCCTATCTTGAG+TGG	+	chr5_2:17499568-17499587	Msa0760850:five_prime_UTR	35.0%
!	TTTTCTCTTCCACTCAAGAT+AGG	-	chr5_2:17499580-17499599	None:intergenic	35.0%
	GAAAAGTGAAAGCTAAGTAG+TGG	+	chr5_2:17499595-17499614	Msa0760850:five_prime_UTR	35.0%
!	AAAGCTAAGTAGTGGAAGAT+GGG	+	chr5_2:17499603-17499622	Msa0760850:exon	35.0%
!	GGATTACTATGAGATTTTGG+AGG	+	chr5_2:17499629-17499648	Msa0760850:CDS	35.0%
	TCAGCATCATTCTTATTGTC+AGG	-	chr5_2:17499732-17499751	None:intergenic	35.0%
!	ACTGATCATTCATGTTTCTG+AGG	+	chr5_2:17499843-17499862	Msa0760850:intron	35.0%
!	GTGTTTGAGATCCAATTTGA+TGG	+	chr5_2:17499967-17499986	Msa0760850:intron	35.0%
	ACTGATTGTAGCCATCAAAT+TGG	-	chr5_2:17499981-17500000	None:intergenic	35.0%
	AATTCTTTGACCCTTCTTAG+GGG	-	chr5_2:17500032-17500051	None:intergenic	35.0%
	TTACATGGAACTCCTTACTT+AGG	-	chr5_2:17500190-17500209	None:intergenic	35.0%
!!	GTAGATTTGTTGTGCGTAAT+TGG	+	chr5_2:17498815-17498834	Msa0760850:five_prime_UTR	35.0%
!	AATGGGTTTGATCAAAACAG+AGG	-	chr5_2:17498994-17499013	None:intergenic	35.0%
	TGATCAAACCCATTACATGA+TGG	+	chr5_2:17499000-17499019	Msa0760850:intron	35.0%
	CATTGTCAGTTCCAGTAATT+AGG	+	chr5_2:17499050-17499069	Msa0760850:intron	35.0%
	CTGTTACAACAAAATTGGAG+AGG	+	chr5_2:17499251-17499270	Msa0760850:intron	35.0%
	GTTCAAAATTCCTGCTAGAT+AGG	+	chr5_2:17499273-17499292	Msa0760850:intron	35.0%
	ACATATTTACCCTATCTAGC+AGG	-	chr5_2:17499286-17499305	None:intergenic	35.0%
!	CTGATGCTGAAATTTTCTGT+TGG	+	chr5_2:17499374-17499393	Msa0760850:intron	35.0%
!	GATGCTGAAATTTTCTGTTG+GGG	+	chr5_2:17499376-17499395	Msa0760850:intron	35.0%
	GTTGATCAGAAACTTCACTT+TGG	-	chr5_2:17499473-17499492	None:intergenic	35.0%
!!!	CAGGTTTTTTGTCTTGTTTC+TGG	-	chr5_2:17499544-17499563	None:intergenic	35.0%
!	GATAGGACTTTCTTTTCTTC+AGG	-	chr5_2:17499563-17499582	None:intergenic	35.0%
	AAAGAAAGTCCTATCTTGAG+TGG	+	chr5_2:17499568-17499587	Msa0760850:five_prime_UTR	35.0%
!	TTTTCTCTTCCACTCAAGAT+AGG	-	chr5_2:17499580-17499599	None:intergenic	35.0%
	GAAAAGTGAAAGCTAAGTAG+TGG	+	chr5_2:17499595-17499614	Msa0760850:five_prime_UTR	35.0%
!	AAAGCTAAGTAGTGGAAGAT+GGG	+	chr5_2:17499603-17499622	Msa0760850:exon	35.0%
!	GGATTACTATGAGATTTTGG+AGG	+	chr5_2:17499629-17499648	Msa0760850:CDS	35.0%
	TCAGCATCATTCTTATTGTC+AGG	-	chr5_2:17499732-17499751	None:intergenic	35.0%
!	ACTGATCATTCATGTTTCTG+AGG	+	chr5_2:17499843-17499862	Msa0760850:intron	35.0%
!	GTGTTTGAGATCCAATTTGA+TGG	+	chr5_2:17499967-17499986	Msa0760850:intron	35.0%
	ACTGATTGTAGCCATCAAAT+TGG	-	chr5_2:17499981-17500000	None:intergenic	35.0%
	AATTCTTTGACCCTTCTTAG+GGG	-	chr5_2:17500032-17500051	None:intergenic	35.0%
	TTACATGGAACTCCTTACTT+AGG	-	chr5_2:17500190-17500209	None:intergenic	35.0%
	AATGTGTGGACTTTCTCTGA+TGG	+	chr5_2:17498750-17498769	Msa0760850:five_prime_UTR	40.0%
	GACTCCATGACCAAGAATAA+AGG	+	chr5_2:17498778-17498797	Msa0760850:five_prime_UTR	40.0%
!!	GATTTGTTGTGCGTAATTGG+AGG	+	chr5_2:17498818-17498837	Msa0760850:five_prime_UTR	40.0%
!	TTTTGACACACCATGTCTGT+CGG	+	chr5_2:17499331-17499350	Msa0760850:intron	40.0%
!	GAAAGCTAAGTAGTGGAAGA+TGG	+	chr5_2:17499602-17499621	Msa0760850:exon	40.0%
!	TAAGTAGTGGAAGATGGGTT+TGG	+	chr5_2:17499608-17499627	Msa0760850:exon	40.0%
	ACTCATATCCGAAGCATATG+TGG	+	chr5_2:17499764-17499783	Msa0760850:CDS	40.0%
	ATACAGTCGGATGAAAACTG+AGG	+	chr5_2:17500004-17500023	Msa0760850:intron	40.0%
	AAATAGCGCCACATTCAGAT+TGG	-	chr5_2:17500120-17500139	None:intergenic	40.0%
	GAGTTCCATGTAAGCATCAT+TGG	+	chr5_2:17500197-17500216	Msa0760850:intron	40.0%
	ATGTAAGCATCATTGGTCCA+GGG	+	chr5_2:17500204-17500223	Msa0760850:intron	40.0%
	AATGTGTGGACTTTCTCTGA+TGG	+	chr5_2:17498750-17498769	Msa0760850:five_prime_UTR	40.0%
	GACTCCATGACCAAGAATAA+AGG	+	chr5_2:17498778-17498797	Msa0760850:five_prime_UTR	40.0%
!!	GATTTGTTGTGCGTAATTGG+AGG	+	chr5_2:17498818-17498837	Msa0760850:five_prime_UTR	40.0%
!	TTTTGACACACCATGTCTGT+CGG	+	chr5_2:17499331-17499350	Msa0760850:intron	40.0%
!	GAAAGCTAAGTAGTGGAAGA+TGG	+	chr5_2:17499602-17499621	Msa0760850:exon	40.0%
!	TAAGTAGTGGAAGATGGGTT+TGG	+	chr5_2:17499608-17499627	Msa0760850:exon	40.0%
	ACTCATATCCGAAGCATATG+TGG	+	chr5_2:17499764-17499783	Msa0760850:CDS	40.0%
	ATACAGTCGGATGAAAACTG+AGG	+	chr5_2:17500004-17500023	Msa0760850:intron	40.0%
	AAATAGCGCCACATTCAGAT+TGG	-	chr5_2:17500120-17500139	None:intergenic	40.0%
	GAGTTCCATGTAAGCATCAT+TGG	+	chr5_2:17500197-17500216	Msa0760850:intron	40.0%
	ATGTAAGCATCATTGGTCCA+GGG	+	chr5_2:17500204-17500223	Msa0760850:intron	40.0%
!	AGAGTGTCTACAAACAGAGC+AGG	-	chr5_2:17498860-17498879	None:intergenic	45.0%
!!!	GATTTTCCATGGTTCTAGCC+TGG	+	chr5_2:17498936-17498955	Msa0760850:five_prime_UTR	45.0%
	GTATGTTTGCCTTATCCACC+AGG	-	chr5_2:17498957-17498976	None:intergenic	45.0%
	GGTGGATAAGGCAAACATAC+AGG	+	chr5_2:17498957-17498976	Msa0760850:five_prime_UTR	45.0%
!!	CTTATCAGGGTGCCATTTCA+TGG	-	chr5_2:17499706-17499725	None:intergenic	45.0%
	AAACTGAGGATCCCCTAAGA+AGG	+	chr5_2:17500018-17500037	Msa0760850:intron	45.0%
	AACTGAGGATCCCCTAAGAA+GGG	+	chr5_2:17500019-17500038	Msa0760850:intron	45.0%
	CTGGACCAATGATGCTTACA+TGG	-	chr5_2:17500205-17500224	None:intergenic	45.0%
	CATGTAAGCATCATTGGTCC+AGG	+	chr5_2:17500203-17500222	Msa0760850:intron	45.0%
!	GTTTTTCCATATACCACGGC+CGG	-	chr5_2:17500238-17500257	None:intergenic	45.0%
!	AGAGTGTCTACAAACAGAGC+AGG	-	chr5_2:17498860-17498879	None:intergenic	45.0%
!!!	GATTTTCCATGGTTCTAGCC+TGG	+	chr5_2:17498936-17498955	Msa0760850:five_prime_UTR	45.0%
	GTATGTTTGCCTTATCCACC+AGG	-	chr5_2:17498957-17498976	None:intergenic	45.0%
	GGTGGATAAGGCAAACATAC+AGG	+	chr5_2:17498957-17498976	Msa0760850:five_prime_UTR	45.0%
!!	CTTATCAGGGTGCCATTTCA+TGG	-	chr5_2:17499706-17499725	None:intergenic	45.0%
	AAACTGAGGATCCCCTAAGA+AGG	+	chr5_2:17500018-17500037	Msa0760850:intron	45.0%
	AACTGAGGATCCCCTAAGAA+GGG	+	chr5_2:17500019-17500038	Msa0760850:intron	45.0%
	CTGGACCAATGATGCTTACA+TGG	-	chr5_2:17500205-17500224	None:intergenic	45.0%
	CATGTAAGCATCATTGGTCC+AGG	+	chr5_2:17500203-17500222	Msa0760850:intron	45.0%
!	GTTTTTCCATATACCACGGC+CGG	-	chr5_2:17500238-17500257	None:intergenic	45.0%
!!	TTTCCATGGTTCTAGCCTGG+TGG	+	chr5_2:17498939-17498958	Msa0760850:five_prime_UTR	50.0%
	TATCCACCAGGCTAGAACCA+TGG	-	chr5_2:17498945-17498964	None:intergenic	50.0%
!!	TGGTTCTAGCCTGGTGGATA+AGG	+	chr5_2:17498945-17498964	Msa0760850:five_prime_UTR	50.0%
!	ACCATGTCTGTCGGTGTTTG+TGG	+	chr5_2:17499340-17499359	Msa0760850:intron	50.0%
	TCCACAAACACCGACAGACA+TGG	-	chr5_2:17499344-17499363	None:intergenic	50.0%
	TTGGTCCAGGGTTTGATCTC+CGG	+	chr5_2:17500216-17500235	Msa0760850:intron	50.0%
!!	TTTCCATGGTTCTAGCCTGG+TGG	+	chr5_2:17498939-17498958	Msa0760850:five_prime_UTR	50.0%
	TATCCACCAGGCTAGAACCA+TGG	-	chr5_2:17498945-17498964	None:intergenic	50.0%
!!	TGGTTCTAGCCTGGTGGATA+AGG	+	chr5_2:17498945-17498964	Msa0760850:five_prime_UTR	50.0%
!	ACCATGTCTGTCGGTGTTTG+TGG	+	chr5_2:17499340-17499359	Msa0760850:intron	50.0%
	TCCACAAACACCGACAGACA+TGG	-	chr5_2:17499344-17499363	None:intergenic	50.0%
	TTGGTCCAGGGTTTGATCTC+CGG	+	chr5_2:17500216-17500235	Msa0760850:intron	50.0%
	TGATCTCCGGCCGTGGTATA+TGG	+	chr5_2:17500229-17500248	Msa0760850:CDS	55.0%
	TGATCTCCGGCCGTGGTATA+TGG	+	chr5_2:17500229-17500248	Msa0760850:CDS	55.0%
	CACGGCCGGAGATCAAACCC+TGG	-	chr5_2:17500224-17500243	None:intergenic	65.0%
	CAGGGTTTGATCTCCGGCCG+TGG	+	chr5_2:17500222-17500241	Msa0760850:intron	65.0%
	CACGGCCGGAGATCAAACCC+TGG	-	chr5_2:17500224-17500243	None:intergenic	65.0%
	CAGGGTTTGATCTCCGGCCG+TGG	+	chr5_2:17500222-17500241	Msa0760850:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	17498701	17500284	17498701	ID=Msa0760850;Name=Msa0760850
chr5_2	mRNA	17498701	17500284	17498701	ID=Msa0760850-mRNA-1;Parent=Msa0760850;Name=Msa0760850-mRNA-1;_AED=0.21;_eAED=0.21;_QI=354\|0.5\|0.33\|0.66\|0.5\|0.33\|3\|0\|74
chr5_2	exon	17498701	17498978	17498701	ID=Msa0760850-mRNA-1:exon:7758;Parent=Msa0760850-mRNA-1
chr5_2	exon	17499545	17499785	17499545	ID=Msa0760850-mRNA-1:exon:7759;Parent=Msa0760850-mRNA-1
chr5_2	exon	17500225	17500284	17500225	ID=Msa0760850-mRNA-1:exon:7760;Parent=Msa0760850-mRNA-1
chr5_2	five_prime_UTR	17498701	17498978	17498701	ID=Msa0760850-mRNA-1:five_prime_utr;Parent=Msa0760850-mRNA-1
chr5_2	five_prime_UTR	17499545	17499620	17499545	ID=Msa0760850-mRNA-1:five_prime_utr;Parent=Msa0760850-mRNA-1
chr5_2	CDS	17499621	17499785	17499621	ID=Msa0760850-mRNA-1:cds;Parent=Msa0760850-mRNA-1
chr5_2	CDS	17500225	17500284	17500225	ID=Msa0760850-mRNA-1:cds;Parent=Msa0760850-mRNA-1

Gene Sequence	>Msa0760850 ATGGGTTTGGATTACTATGAGATTTTGGAGGTTGACAAGAACGCTACAGATGATGAGTTGAAGAAAGCATATAGAAAACTTGCCATGAAATGGCACCCTGATAAGAATCCTGACAATAAGAATGATGCTGAGACTAAATTCAAACTCATATCCGAAGCATATGTGGGTTTGATCTCCGGCCGTGGTATATGGAAAAACATATCTTATGATAGGTCTACCTCTTAA
Protein sequence	>Msa0760850 MGLDYYEILEVDKNATDDELKKAYRKLAMKWHPDKNPDNKNDAETKFKLISEAYVGLISGRGIWKNISYDRSTS*