AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0763120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0763120 MtrunA17_Chr2g0282761 98.246 57 1 0 1 57 1 57 1.15e-33 117
Msa0763120 MtrunA17_Chr4g0040501 84.211 57 9 0 1 57 1 57 9.35e-28 102
Msa0763120 MtrunA17_Chr2g0282741 82.456 57 10 0 1 57 1 57 5.13e-27 99.8
Msa0763120 MtrunA17_Chr4g0006391 75.439 57 14 0 1 57 1 57 2.41e-26 98.2
Msa0763120 MtrunA17_Chr3g0138471 77.193 57 13 0 1 57 1 57 1.07e-25 96.3
Msa0763120 MtrunA17_Chr2g0324921 75.439 57 14 0 1 57 1 57 3.47e-25 95.1
Msa0763120 MtrunA17_Chr7g0263971 57.407 54 23 0 4 57 369 422 1.09e-16 72.4
Msa0763120 MtrunA17_Chr7g0269841 62.264 53 19 1 5 57 27 78 1.12e-15 69.3
Msa0763120 MtrunA17_Chr5g0415731 47.368 76 30 2 4 72 5 77 3.95e-13 60.1
Msa0763120 MtrunA17_Chr5g0427221 51.852 54 25 1 4 57 48 100 1.85e-12 58.2
Msa0763120 MtrunA17_Chr3g0093111 50.000 56 27 1 3 57 1 56 2.31e-12 60.1
Msa0763120 MtrunA17_Chr6g0460461 41.270 63 33 1 3 61 1 63 2.61e-12 58.9
Msa0763120 MtrunA17_Chr3g0123861 59.259 54 20 1 4 57 82 133 2.95e-12 59.7
Msa0763120 MtrunA17_Chr5g0426951 47.059 68 32 2 4 71 83 146 3.03e-12 59.7
Msa0763120 MtrunA17_Chr6g0460411 41.270 63 33 1 3 61 1 63 4.08e-12 58.5
Msa0763120 MtrunA17_Chr7g0271891 44.643 56 28 1 5 57 17 72 4.24e-12 57.8
Msa0763120 MtrunA17_Chr3g0091361 48.214 56 29 0 3 58 4 59 4.45e-12 58.5
Msa0763120 MtrunA17_Chr4g0021141 48.148 54 27 1 4 57 22 74 8.27e-12 56.2
Msa0763120 MtrunA17_Chr3g0087741 51.852 54 25 1 4 57 89 141 2.37e-11 57.4
Msa0763120 MtrunA17_Chr6g0460381 39.683 63 34 1 3 61 2 64 3.69e-11 55.8
Msa0763120 MtrunA17_Chr1g0159491 45.455 55 29 1 3 57 5 58 4.86e-11 53.1
Msa0763120 MtrunA17_Chr2g0307061 50.000 54 25 1 4 57 110 161 5.71e-11 56.2
Msa0763120 MtrunA17_Chr8g0334811 56.604 53 18 1 5 57 15 62 6.22e-11 56.2
Msa0763120 MtrunA17_Chr4g0010781 46.269 67 32 2 5 71 87 149 6.42e-11 56.2
Msa0763120 MtrunA17_Chr6g0478651 55.172 58 20 2 2 57 66 119 6.82e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0763120 AT3G08910.1 77.193 57 13 0 1 57 1 57 1.85e-26 98.6
Msa0763120 AT5G01390.4 75.439 57 14 0 1 57 1 57 2.26e-26 96.7
Msa0763120 AT5G01390.3 75.439 57 14 0 1 57 1 57 2.48e-26 96.7
Msa0763120 AT5G01390.2 75.439 57 14 0 1 57 1 57 5.70e-26 96.3
Msa0763120 AT5G01390.1 75.439 57 14 0 1 57 1 57 1.01e-25 96.7
Msa0763120 AT4G28480.2 75.439 57 14 0 1 57 1 57 1.13e-25 95.9
Msa0763120 AT2G20560.1 75.439 57 14 0 1 57 1 57 1.29e-25 96.7
Msa0763120 AT4G28480.1 75.439 57 14 0 1 57 1 57 2.29e-25 95.9
Msa0763120 AT5G25530.2 75.439 57 14 0 1 57 1 57 3.25e-25 95.5
Msa0763120 AT5G25530.1 76.364 55 13 0 1 55 1 55 5.53e-25 95.1
Msa0763120 AT1G10350.1 64.912 57 20 0 1 57 1 57 6.56e-22 86.7
Msa0763120 AT1G59725.1 65.517 58 19 1 1 57 1 58 2.87e-20 82.0
Msa0763120 AT3G47940.1 67.241 58 18 1 1 57 1 58 5.31e-20 81.6
Msa0763120 AT2G41000.2 47.458 59 27 1 3 57 2 60 1.83e-14 65.1
Msa0763120 AT2G41000.1 47.458 59 27 1 3 57 2 60 3.31e-14 63.9
Msa0763120 AT5G03160.1 53.704 54 25 0 4 57 370 423 4.26e-14 65.5
Msa0763120 AT3G62600.1 53.571 56 25 1 2 57 24 78 5.51e-13 62.4
Msa0763120 AT2G22360.1 54.412 68 26 2 4 71 86 148 7.75e-13 62.0
Msa0763120 AT5G48030.1 57.407 54 22 1 4 57 94 146 1.24e-12 61.2
Msa0763120 AT4G39960.1 52.941 68 27 2 4 71 85 147 2.23e-12 60.5
Msa0763120 AT4G39960.2 52.941 68 27 2 4 71 85 147 2.23e-12 60.5
Msa0763120 AT1G56300.2 48.214 56 26 1 5 57 14 69 3.40e-12 57.4
Msa0763120 AT3G17830.1 51.389 72 26 3 2 71 61 125 3.63e-12 60.1
Msa0763120 AT1G56300.1 48.214 56 26 1 5 57 14 69 4.64e-12 57.8
Msa0763120 AT5G16650.1 52.727 55 25 1 4 58 11 64 8.44e-12 56.6
Msa0763120 AT5G16650.2 52.727 55 25 1 4 58 11 64 8.81e-12 57.4
Msa0763120 AT1G59980.2 51.923 52 24 1 6 57 25 75 9.57e-12 58.9
Msa0763120 AT1G59980.1 51.923 52 24 1 6 57 25 75 2.26e-11 57.8
Msa0763120 AT1G68370.1 54.902 51 22 1 4 54 17 66 4.36e-11 57.0

Find 30 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGATTACTATGAGATTT+TGG 0.205034 5_2:+21833978 Msa0763120:CDS
GTAGATTTGTTGTGCGTAAT+TGG 0.247560 5_2:+21833167 Msa0763120:five_prime_UTR
TTATTGTCAGGATTCTTATC+AGG 0.253732 5_2:-21834069 None:intergenic
ACAGATTAAACCTTTATTCT+TGG 0.256184 5_2:-21833140 None:intergenic
TGATCTCCGGCCGTGGTATA+TGG 0.302314 5_2:+21834581 Msa0763120:CDS
CTTATCAGGGTGCCATTTCA+TGG 0.338061 5_2:-21834055 None:intergenic
GACTCCATGACCAAGAATAA+AGG 0.373010 5_2:+21833130 Msa0763120:five_prime_UTR
TTGGTCCAGGGTTTGATCTC+CGG 0.395380 5_2:+21834568 Msa0763120:intron
ATAGAAAACTTGCCATGAAA+TGG 0.412382 5_2:+21834043 Msa0763120:CDS
TGGTTCTAGCCTGGTGGATA+AGG 0.413461 5_2:+21833297 Msa0763120:five_prime_UTR
TCAGCATCATTCTTATTGTC+AGG 0.435149 5_2:-21834081 None:intergenic
TAAACCTTTATTCTTGGTCA+TGG 0.437062 5_2:-21833134 None:intergenic
GAAAGCTAAGTAGTGGAAGA+TGG 0.444821 5_2:+21833954 Msa0763120:exon
TATTGTCAGGATTCTTATCA+GGG 0.462954 5_2:-21834068 None:intergenic
TAAGTAGTGGAAGATGGGTT+TGG 0.464260 5_2:+21833960 Msa0763120:exon
TACATATACCTCATATGCTT+CGG 0.471997 5_2:-21834124 None:intergenic
GTAACTAAGATCAATTTAAG+AGG 0.474293 5_2:-21834629 None:intergenic
ACTCATATCCGAAGCATATG+AGG 0.475266 5_2:+21834116 Msa0763120:CDS
AAAGCTAAGTAGTGGAAGAT+GGG 0.475347 5_2:+21833955 Msa0763120:exon
GAAAAGTGAAAGCTAAGTAG+TGG 0.495138 5_2:+21833947 Msa0763120:five_prime_UTR
GGTGGATAAGGCAAACATAC+AGG 0.505654 5_2:+21833309 Msa0763120:five_prime_UTR
CAGGGTTTGATCTCCGGCCG+TGG 0.523748 5_2:+21834574 Msa0763120:intron
GATTTGTTGTGCGTAATTGG+AGG 0.553928 5_2:+21833170 Msa0763120:five_prime_UTR
GTATGTTTGCCTTATCCACC+AGG 0.559232 5_2:-21833306 None:intergenic
AAAGAAAGTCCTATCTTGAG+TGG 0.579591 5_2:+21833920 Msa0763120:five_prime_UTR
TATCCACCAGGCTAGAACCA+TGG 0.582402 5_2:-21833294 None:intergenic
AATGTGTGGACTTTCTCTGA+TGG 0.591588 5_2:+21833102 Msa0763120:five_prime_UTR
TTTCCATGGTTCTAGCCTGG+TGG 0.591889 5_2:+21833291 Msa0763120:five_prime_UTR
AGAGTGTCTACAAACAGAGC+AGG 0.623091 5_2:-21833209 None:intergenic
GACTGAAATGTATTAATGTG+TGG 0.658305 5_2:+21833088 Msa0763120:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTGATTACTATTTATA+TGG + chr5_2:21833490-21833509 Msa0763120:intron 15.0%
!! AATAATAGATAAAATATCTG+AGG - chr5_2:21833523-21833542 None:intergenic 15.0%
!!! TTTTCTGATTACTATTTATA+TGG + chr5_2:21833490-21833509 Msa0763120:intron 15.0%
!! AATAATAGATAAAATATCTG+AGG - chr5_2:21833523-21833542 None:intergenic 15.0%
!! AAAAATAACCATCATGTAAT+GGG - chr5_2:21833363-21833382 None:intergenic 20.0%
!! TAAAAATAACCATCATGTAA+TGG - chr5_2:21833364-21833383 None:intergenic 20.0%
!! TGCAAAATTAACATTTAAGT+TGG + chr5_2:21833458-21833477 Msa0763120:intron 20.0%
!!! CTATTATTGTGTTGTTTTAA+CGG + chr5_2:21833535-21833554 Msa0763120:intron 20.0%
!! TTTATCTGTTACAACAAAAT+TGG + chr5_2:21833598-21833617 Msa0763120:intron 20.0%
!! TGTAATGATTATTATTGAAC+AGG - chr5_2:21833869-21833888 None:intergenic 20.0%
!!! AAATTGAAAATTGAGGATTT+TGG - chr5_2:21834434-21834453 None:intergenic 20.0%
!! GGAAAAAAAATTGAAAATTG+AGG - chr5_2:21834441-21834460 None:intergenic 20.0%
!! AAAAATAACCATCATGTAAT+GGG - chr5_2:21833363-21833382 None:intergenic 20.0%
!! TAAAAATAACCATCATGTAA+TGG - chr5_2:21833364-21833383 None:intergenic 20.0%
!! TGCAAAATTAACATTTAAGT+TGG + chr5_2:21833458-21833477 Msa0763120:intron 20.0%
!!! CTATTATTGTGTTGTTTTAA+CGG + chr5_2:21833535-21833554 Msa0763120:intron 20.0%
!! TTTATCTGTTACAACAAAAT+TGG + chr5_2:21833598-21833617 Msa0763120:intron 20.0%
!! TGTAATGATTATTATTGAAC+AGG - chr5_2:21833869-21833888 None:intergenic 20.0%
!!! AAATTGAAAATTGAGGATTT+TGG - chr5_2:21834434-21834453 None:intergenic 20.0%
!! GGAAAAAAAATTGAAAATTG+AGG - chr5_2:21834441-21834460 None:intergenic 20.0%
! ACAGATTAAACCTTTATTCT+TGG - chr5_2:21833143-21833162 None:intergenic 25.0%
! AATGTGGTATACTTAAAGTT+TGG - chr5_2:21833428-21833447 None:intergenic 25.0%
! ATTCTCACTATACTTTAACA+AGG + chr5_2:21833561-21833580 Msa0763120:intron 25.0%
! ATTATTATTGAACAGGACTA+TGG - chr5_2:21833862-21833881 None:intergenic 25.0%
!! TTTGGATTACTATGAGATTT+TGG + chr5_2:21833978-21833997 Msa0763120:CDS 25.0%
! GTTTAATTGCTACAAAACTA+TGG + chr5_2:21834169-21834188 Msa0763120:intron 25.0%
!!! ATTATCTTGTTTCTCTGTTT+TGG + chr5_2:21834239-21834258 Msa0763120:intron 25.0%
!!! AAAATTGAGGATTTTGGTAA+GGG - chr5_2:21834428-21834447 None:intergenic 25.0%
!! ATATGTTTTTCCATATACCA+CGG - chr5_2:21834594-21834613 None:intergenic 25.0%
! GGAAAAACATATCTTATGAT+AGG + chr5_2:21834602-21834621 Msa0763120:CDS 25.0%
! ACAGATTAAACCTTTATTCT+TGG - chr5_2:21833143-21833162 None:intergenic 25.0%
! AATGTGGTATACTTAAAGTT+TGG - chr5_2:21833428-21833447 None:intergenic 25.0%
! ATTCTCACTATACTTTAACA+AGG + chr5_2:21833561-21833580 Msa0763120:intron 25.0%
! ATTATTATTGAACAGGACTA+TGG - chr5_2:21833862-21833881 None:intergenic 25.0%
!! TTTGGATTACTATGAGATTT+TGG + chr5_2:21833978-21833997 Msa0763120:CDS 25.0%
! GTTTAATTGCTACAAAACTA+TGG + chr5_2:21834169-21834188 Msa0763120:intron 25.0%
!!! ATTATCTTGTTTCTCTGTTT+TGG + chr5_2:21834239-21834258 Msa0763120:intron 25.0%
!!! AAAATTGAGGATTTTGGTAA+GGG - chr5_2:21834428-21834447 None:intergenic 25.0%
!! ATATGTTTTTCCATATACCA+CGG - chr5_2:21834594-21834613 None:intergenic 25.0%
! GGAAAAACATATCTTATGAT+AGG + chr5_2:21834602-21834621 Msa0763120:CDS 25.0%
GACTGAAATGTATTAATGTG+TGG + chr5_2:21833088-21833107 Msa0763120:five_prime_UTR 30.0%
TAAACCTTTATTCTTGGTCA+TGG - chr5_2:21833137-21833156 None:intergenic 30.0%
TTACAGTTTCAAAAAAGGTC+GGG - chr5_2:21833244-21833263 None:intergenic 30.0%
GTTACAGTTTCAAAAAAGGT+CGG - chr5_2:21833245-21833264 None:intergenic 30.0%
CATCGTTACAGTTTCAAAAA+AGG - chr5_2:21833249-21833268 None:intergenic 30.0%
! TGTTGCAAAAAGATTTTCCA+TGG + chr5_2:21833277-21833296 Msa0763120:five_prime_UTR 30.0%
TTAAAGTTTGGCCTAATTAC+TGG - chr5_2:21833416-21833435 None:intergenic 30.0%
TTTGCACTATGAAAAGAATG+TGG - chr5_2:21833444-21833463 None:intergenic 30.0%
TTCAAAATTCCTGCTAGATA+GGG + chr5_2:21833626-21833645 Msa0763120:intron 30.0%
! TGATGCTGAAATTTTCTGTT+GGG + chr5_2:21833727-21833746 Msa0763120:intron 30.0%
ATAGAAAACTTGCCATGAAA+TGG + chr5_2:21834043-21834062 Msa0763120:CDS 30.0%
TATTGTCAGGATTCTTATCA+GGG - chr5_2:21834071-21834090 None:intergenic 30.0%
TTATTGTCAGGATTCTTATC+AGG - chr5_2:21834072-21834091 None:intergenic 30.0%
TACATATACCTCATATGCTT+CGG - chr5_2:21834127-21834146 None:intergenic 30.0%
!! TGTTTACTGTCTTTTGTTTG+AGG + chr5_2:21834285-21834304 Msa0763120:intron 30.0%
TACAATCAGTGATATACAGT+CGG + chr5_2:21834343-21834362 Msa0763120:intron 30.0%
AAATTCTTTGACCCTTCTTA+GGG - chr5_2:21834385-21834404 None:intergenic 30.0%
AAAATTCTTTGACCCTTCTT+AGG - chr5_2:21834386-21834405 None:intergenic 30.0%
!! GAAAATTGAGGATTTTGGTA+AGG - chr5_2:21834429-21834448 None:intergenic 30.0%
ACATTCAGATTGGATAATGA+AGG - chr5_2:21834462-21834481 None:intergenic 30.0%
TTCATTATCCAATCTGAATG+TGG + chr5_2:21834461-21834480 Msa0763120:intron 30.0%
! TTTGACTTTCATCCTAAGTA+AGG + chr5_2:21834527-21834546 Msa0763120:intron 30.0%
GACTGAAATGTATTAATGTG+TGG + chr5_2:21833088-21833107 Msa0763120:five_prime_UTR 30.0%
TAAACCTTTATTCTTGGTCA+TGG - chr5_2:21833137-21833156 None:intergenic 30.0%
TTACAGTTTCAAAAAAGGTC+GGG - chr5_2:21833244-21833263 None:intergenic 30.0%
GTTACAGTTTCAAAAAAGGT+CGG - chr5_2:21833245-21833264 None:intergenic 30.0%
CATCGTTACAGTTTCAAAAA+AGG - chr5_2:21833249-21833268 None:intergenic 30.0%
! TGTTGCAAAAAGATTTTCCA+TGG + chr5_2:21833277-21833296 Msa0763120:five_prime_UTR 30.0%
TTAAAGTTTGGCCTAATTAC+TGG - chr5_2:21833416-21833435 None:intergenic 30.0%
TTTGCACTATGAAAAGAATG+TGG - chr5_2:21833444-21833463 None:intergenic 30.0%
TTCAAAATTCCTGCTAGATA+GGG + chr5_2:21833626-21833645 Msa0763120:intron 30.0%
! TGATGCTGAAATTTTCTGTT+GGG + chr5_2:21833727-21833746 Msa0763120:intron 30.0%
ATAGAAAACTTGCCATGAAA+TGG + chr5_2:21834043-21834062 Msa0763120:CDS 30.0%
TATTGTCAGGATTCTTATCA+GGG - chr5_2:21834071-21834090 None:intergenic 30.0%
TTATTGTCAGGATTCTTATC+AGG - chr5_2:21834072-21834091 None:intergenic 30.0%
TACATATACCTCATATGCTT+CGG - chr5_2:21834127-21834146 None:intergenic 30.0%
!! TGTTTACTGTCTTTTGTTTG+AGG + chr5_2:21834285-21834304 Msa0763120:intron 30.0%
TACAATCAGTGATATACAGT+CGG + chr5_2:21834343-21834362 Msa0763120:intron 30.0%
AAATTCTTTGACCCTTCTTA+GGG - chr5_2:21834385-21834404 None:intergenic 30.0%
AAAATTCTTTGACCCTTCTT+AGG - chr5_2:21834386-21834405 None:intergenic 30.0%
!! GAAAATTGAGGATTTTGGTA+AGG - chr5_2:21834429-21834448 None:intergenic 30.0%
ACATTCAGATTGGATAATGA+AGG - chr5_2:21834462-21834481 None:intergenic 30.0%
TTCATTATCCAATCTGAATG+TGG + chr5_2:21834461-21834480 Msa0763120:intron 30.0%
! TTTGACTTTCATCCTAAGTA+AGG + chr5_2:21834527-21834546 Msa0763120:intron 30.0%
!! GTAGATTTGTTGTGCGTAAT+TGG + chr5_2:21833167-21833186 Msa0763120:five_prime_UTR 35.0%
! AATGGGTTTGATCAAAACAG+AGG - chr5_2:21833346-21833365 None:intergenic 35.0%
TGATCAAACCCATTACATGA+TGG + chr5_2:21833352-21833371 Msa0763120:intron 35.0%
CATTGTCAGTTCCAGTAATT+AGG + chr5_2:21833402-21833421 Msa0763120:intron 35.0%
CTGTTACAACAAAATTGGAG+AGG + chr5_2:21833603-21833622 Msa0763120:intron 35.0%
GTTCAAAATTCCTGCTAGAT+AGG + chr5_2:21833625-21833644 Msa0763120:intron 35.0%
ACATATTTACCCTATCTAGC+AGG - chr5_2:21833638-21833657 None:intergenic 35.0%
! CTGATGCTGAAATTTTCTGT+TGG + chr5_2:21833726-21833745 Msa0763120:intron 35.0%
! GATGCTGAAATTTTCTGTTG+GGG + chr5_2:21833728-21833747 Msa0763120:intron 35.0%
GTTGATCAGAAACTTCACTT+TGG - chr5_2:21833825-21833844 None:intergenic 35.0%
!!! CAGGTTTTTTGTCTTGTTTC+TGG - chr5_2:21833896-21833915 None:intergenic 35.0%
! GATAGGACTTTCTTTTCTTC+AGG - chr5_2:21833915-21833934 None:intergenic 35.0%
AAAGAAAGTCCTATCTTGAG+TGG + chr5_2:21833920-21833939 Msa0763120:five_prime_UTR 35.0%
! TTTTCTCTTCCACTCAAGAT+AGG - chr5_2:21833932-21833951 None:intergenic 35.0%
GAAAAGTGAAAGCTAAGTAG+TGG + chr5_2:21833947-21833966 Msa0763120:five_prime_UTR 35.0%
! AAAGCTAAGTAGTGGAAGAT+GGG + chr5_2:21833955-21833974 Msa0763120:exon 35.0%
! GGATTACTATGAGATTTTGG+AGG + chr5_2:21833981-21834000 Msa0763120:CDS 35.0%
TCAGCATCATTCTTATTGTC+AGG - chr5_2:21834084-21834103 None:intergenic 35.0%
! ACTGATCATTCATGTTTCTG+AGG + chr5_2:21834195-21834214 Msa0763120:intron 35.0%
! GTGTTTGAGATCCAATTTGA+TGG + chr5_2:21834319-21834338 Msa0763120:intron 35.0%
ACTGATTGTAGCCATCAAAT+TGG - chr5_2:21834333-21834352 None:intergenic 35.0%
AATTCTTTGACCCTTCTTAG+GGG - chr5_2:21834384-21834403 None:intergenic 35.0%
TTACATGGAACTCCTTACTT+AGG - chr5_2:21834542-21834561 None:intergenic 35.0%
!! GTAGATTTGTTGTGCGTAAT+TGG + chr5_2:21833167-21833186 Msa0763120:five_prime_UTR 35.0%
! AATGGGTTTGATCAAAACAG+AGG - chr5_2:21833346-21833365 None:intergenic 35.0%
TGATCAAACCCATTACATGA+TGG + chr5_2:21833352-21833371 Msa0763120:intron 35.0%
CATTGTCAGTTCCAGTAATT+AGG + chr5_2:21833402-21833421 Msa0763120:intron 35.0%
CTGTTACAACAAAATTGGAG+AGG + chr5_2:21833603-21833622 Msa0763120:intron 35.0%
GTTCAAAATTCCTGCTAGAT+AGG + chr5_2:21833625-21833644 Msa0763120:intron 35.0%
ACATATTTACCCTATCTAGC+AGG - chr5_2:21833638-21833657 None:intergenic 35.0%
! CTGATGCTGAAATTTTCTGT+TGG + chr5_2:21833726-21833745 Msa0763120:intron 35.0%
! GATGCTGAAATTTTCTGTTG+GGG + chr5_2:21833728-21833747 Msa0763120:intron 35.0%
GTTGATCAGAAACTTCACTT+TGG - chr5_2:21833825-21833844 None:intergenic 35.0%
!!! CAGGTTTTTTGTCTTGTTTC+TGG - chr5_2:21833896-21833915 None:intergenic 35.0%
! GATAGGACTTTCTTTTCTTC+AGG - chr5_2:21833915-21833934 None:intergenic 35.0%
AAAGAAAGTCCTATCTTGAG+TGG + chr5_2:21833920-21833939 Msa0763120:five_prime_UTR 35.0%
! TTTTCTCTTCCACTCAAGAT+AGG - chr5_2:21833932-21833951 None:intergenic 35.0%
GAAAAGTGAAAGCTAAGTAG+TGG + chr5_2:21833947-21833966 Msa0763120:five_prime_UTR 35.0%
! AAAGCTAAGTAGTGGAAGAT+GGG + chr5_2:21833955-21833974 Msa0763120:exon 35.0%
! GGATTACTATGAGATTTTGG+AGG + chr5_2:21833981-21834000 Msa0763120:CDS 35.0%
TCAGCATCATTCTTATTGTC+AGG - chr5_2:21834084-21834103 None:intergenic 35.0%
! ACTGATCATTCATGTTTCTG+AGG + chr5_2:21834195-21834214 Msa0763120:intron 35.0%
! GTGTTTGAGATCCAATTTGA+TGG + chr5_2:21834319-21834338 Msa0763120:intron 35.0%
ACTGATTGTAGCCATCAAAT+TGG - chr5_2:21834333-21834352 None:intergenic 35.0%
AATTCTTTGACCCTTCTTAG+GGG - chr5_2:21834384-21834403 None:intergenic 35.0%
TTACATGGAACTCCTTACTT+AGG - chr5_2:21834542-21834561 None:intergenic 35.0%
AATGTGTGGACTTTCTCTGA+TGG + chr5_2:21833102-21833121 Msa0763120:five_prime_UTR 40.0%
GACTCCATGACCAAGAATAA+AGG + chr5_2:21833130-21833149 Msa0763120:five_prime_UTR 40.0%
!! GATTTGTTGTGCGTAATTGG+AGG + chr5_2:21833170-21833189 Msa0763120:five_prime_UTR 40.0%
! TTTTGACACACCATGTCTGT+CGG + chr5_2:21833683-21833702 Msa0763120:intron 40.0%
! GAAAGCTAAGTAGTGGAAGA+TGG + chr5_2:21833954-21833973 Msa0763120:exon 40.0%
! TAAGTAGTGGAAGATGGGTT+TGG + chr5_2:21833960-21833979 Msa0763120:exon 40.0%
ACTCATATCCGAAGCATATG+AGG + chr5_2:21834116-21834135 Msa0763120:CDS 40.0%
ATACAGTCGGATGAAAACTG+AGG + chr5_2:21834356-21834375 Msa0763120:intron 40.0%
AAATAGCGCCACATTCAGAT+TGG - chr5_2:21834472-21834491 None:intergenic 40.0%
GAGTTCCATGTAAGCATCAT+TGG + chr5_2:21834549-21834568 Msa0763120:intron 40.0%
ATGTAAGCATCATTGGTCCA+GGG + chr5_2:21834556-21834575 Msa0763120:intron 40.0%
AATGTGTGGACTTTCTCTGA+TGG + chr5_2:21833102-21833121 Msa0763120:five_prime_UTR 40.0%
GACTCCATGACCAAGAATAA+AGG + chr5_2:21833130-21833149 Msa0763120:five_prime_UTR 40.0%
!! GATTTGTTGTGCGTAATTGG+AGG + chr5_2:21833170-21833189 Msa0763120:five_prime_UTR 40.0%
! TTTTGACACACCATGTCTGT+CGG + chr5_2:21833683-21833702 Msa0763120:intron 40.0%
! GAAAGCTAAGTAGTGGAAGA+TGG + chr5_2:21833954-21833973 Msa0763120:exon 40.0%
! TAAGTAGTGGAAGATGGGTT+TGG + chr5_2:21833960-21833979 Msa0763120:exon 40.0%
ACTCATATCCGAAGCATATG+AGG + chr5_2:21834116-21834135 Msa0763120:CDS 40.0%
ATACAGTCGGATGAAAACTG+AGG + chr5_2:21834356-21834375 Msa0763120:intron 40.0%
AAATAGCGCCACATTCAGAT+TGG - chr5_2:21834472-21834491 None:intergenic 40.0%
GAGTTCCATGTAAGCATCAT+TGG + chr5_2:21834549-21834568 Msa0763120:intron 40.0%
ATGTAAGCATCATTGGTCCA+GGG + chr5_2:21834556-21834575 Msa0763120:intron 40.0%
! AGAGTGTCTACAAACAGAGC+AGG - chr5_2:21833212-21833231 None:intergenic 45.0%
!!! GATTTTCCATGGTTCTAGCC+TGG + chr5_2:21833288-21833307 Msa0763120:five_prime_UTR 45.0%
GTATGTTTGCCTTATCCACC+AGG - chr5_2:21833309-21833328 None:intergenic 45.0%
GGTGGATAAGGCAAACATAC+AGG + chr5_2:21833309-21833328 Msa0763120:five_prime_UTR 45.0%
!! CTTATCAGGGTGCCATTTCA+TGG - chr5_2:21834058-21834077 None:intergenic 45.0%
AAACTGAGGATCCCCTAAGA+AGG + chr5_2:21834370-21834389 Msa0763120:intron 45.0%
AACTGAGGATCCCCTAAGAA+GGG + chr5_2:21834371-21834390 Msa0763120:intron 45.0%
CTGGACCAATGATGCTTACA+TGG - chr5_2:21834557-21834576 None:intergenic 45.0%
CATGTAAGCATCATTGGTCC+AGG + chr5_2:21834555-21834574 Msa0763120:intron 45.0%
! GTTTTTCCATATACCACGGC+CGG - chr5_2:21834590-21834609 None:intergenic 45.0%
! AGAGTGTCTACAAACAGAGC+AGG - chr5_2:21833212-21833231 None:intergenic 45.0%
!!! GATTTTCCATGGTTCTAGCC+TGG + chr5_2:21833288-21833307 Msa0763120:five_prime_UTR 45.0%
GTATGTTTGCCTTATCCACC+AGG - chr5_2:21833309-21833328 None:intergenic 45.0%
GGTGGATAAGGCAAACATAC+AGG + chr5_2:21833309-21833328 Msa0763120:five_prime_UTR 45.0%
!! CTTATCAGGGTGCCATTTCA+TGG - chr5_2:21834058-21834077 None:intergenic 45.0%
AAACTGAGGATCCCCTAAGA+AGG + chr5_2:21834370-21834389 Msa0763120:intron 45.0%
AACTGAGGATCCCCTAAGAA+GGG + chr5_2:21834371-21834390 Msa0763120:intron 45.0%
CTGGACCAATGATGCTTACA+TGG - chr5_2:21834557-21834576 None:intergenic 45.0%
CATGTAAGCATCATTGGTCC+AGG + chr5_2:21834555-21834574 Msa0763120:intron 45.0%
! GTTTTTCCATATACCACGGC+CGG - chr5_2:21834590-21834609 None:intergenic 45.0%
!! TTTCCATGGTTCTAGCCTGG+TGG + chr5_2:21833291-21833310 Msa0763120:five_prime_UTR 50.0%
TATCCACCAGGCTAGAACCA+TGG - chr5_2:21833297-21833316 None:intergenic 50.0%
!! TGGTTCTAGCCTGGTGGATA+AGG + chr5_2:21833297-21833316 Msa0763120:five_prime_UTR 50.0%
! ACCATGTCTGTCGGTGTTTG+TGG + chr5_2:21833692-21833711 Msa0763120:intron 50.0%
TCCACAAACACCGACAGACA+TGG - chr5_2:21833696-21833715 None:intergenic 50.0%
TTGGTCCAGGGTTTGATCTC+CGG + chr5_2:21834568-21834587 Msa0763120:intron 50.0%
!! TTTCCATGGTTCTAGCCTGG+TGG + chr5_2:21833291-21833310 Msa0763120:five_prime_UTR 50.0%
TATCCACCAGGCTAGAACCA+TGG - chr5_2:21833297-21833316 None:intergenic 50.0%
!! TGGTTCTAGCCTGGTGGATA+AGG + chr5_2:21833297-21833316 Msa0763120:five_prime_UTR 50.0%
! ACCATGTCTGTCGGTGTTTG+TGG + chr5_2:21833692-21833711 Msa0763120:intron 50.0%
TCCACAAACACCGACAGACA+TGG - chr5_2:21833696-21833715 None:intergenic 50.0%
TTGGTCCAGGGTTTGATCTC+CGG + chr5_2:21834568-21834587 Msa0763120:intron 50.0%
TGATCTCCGGCCGTGGTATA+TGG + chr5_2:21834581-21834600 Msa0763120:CDS 55.0%
TGATCTCCGGCCGTGGTATA+TGG + chr5_2:21834581-21834600 Msa0763120:CDS 55.0%
CACGGCCGGAGATCAAACCC+TGG - chr5_2:21834576-21834595 None:intergenic 65.0%
CAGGGTTTGATCTCCGGCCG+TGG + chr5_2:21834574-21834593 Msa0763120:intron 65.0%
CACGGCCGGAGATCAAACCC+TGG - chr5_2:21834576-21834595 None:intergenic 65.0%
CAGGGTTTGATCTCCGGCCG+TGG + chr5_2:21834574-21834593 Msa0763120:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 21833053 21834636 21833053 ID=Msa0763120;Name=Msa0763120
chr5_2 mRNA 21833053 21834636 21833053 ID=Msa0763120-mRNA-1;Parent=Msa0763120;Name=Msa0763120-mRNA-1;_AED=0.21;_eAED=0.21;_QI=354|0.5|0.33|0.66|0.5|0.33|3|0|74
chr5_2 exon 21833053 21833330 21833053 ID=Msa0763120-mRNA-1:exon:9064;Parent=Msa0763120-mRNA-1
chr5_2 exon 21833897 21834137 21833897 ID=Msa0763120-mRNA-1:exon:9065;Parent=Msa0763120-mRNA-1
chr5_2 exon 21834577 21834636 21834577 ID=Msa0763120-mRNA-1:exon:9066;Parent=Msa0763120-mRNA-1
chr5_2 five_prime_UTR 21833053 21833330 21833053 ID=Msa0763120-mRNA-1:five_prime_utr;Parent=Msa0763120-mRNA-1
chr5_2 five_prime_UTR 21833897 21833972 21833897 ID=Msa0763120-mRNA-1:five_prime_utr;Parent=Msa0763120-mRNA-1
chr5_2 CDS 21833973 21834137 21833973 ID=Msa0763120-mRNA-1:cds;Parent=Msa0763120-mRNA-1
chr5_2 CDS 21834577 21834636 21834577 ID=Msa0763120-mRNA-1:cds;Parent=Msa0763120-mRNA-1
Gene Sequence

>Msa0763120

ATGGGTTTGGATTACTATGAGATTTTGGAGGTTGACAAGAACGCTACAGATGATGAGTTGAAGAAAGCATATAGAAAACTTGCCATGAAATGGCACCCTGATAAGAATCCTGACAATAAGAATGATGCTGAGACTAAATTCAAACTCATATCCGAAGCATATGAGGGTTTGATCTCCGGCCGTGGTATATGGAAAAACATATCTTATGATAGGTCTACCTCTTAA
Protein sequence

>Msa0763120

MGLDYYEILEVDKNATDDELKKAYRKLAMKWHPDKNPDNKNDAETKFKLISEAYEGLISGRGIWKNISYDRSTS*