AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0761280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0761280 MtrunA17_Chr5g0411511 93.750 128 8 0 1 128 2 129 7.10e-77 223
Msa0761280 MtrunA17_Chr8g0366891 61.983 121 40 4 3 122 1 116 7.35e-45 142
Msa0761280 MtrunA17_Chr7g0249111 77.612 67 4 1 1 67 1 56 1.45e-20 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0761280 AT5G65660.1 57.480 127 52 2 2 128 1 125 1.47e-43 139

Find 77 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGGATTAACTATAACTTA+TGG 0.283702 5_2:-18443310 None:intergenic
GGACTTGGAAGGTGGGGATT+GGG 0.296152 5_2:-18443593 None:intergenic
AATGGTGATCACGGTGGTTT+GGG 0.312224 5_2:-18444041 None:intergenic
TGGACTTGGAAGGTGGGGAT+TGG 0.315598 5_2:-18443594 None:intergenic
GTGTGTAGTTGAGGAAATTA+AGG 0.324813 5_2:-18443332 None:intergenic
CAATGGTGATCACGGTGGTT+TGG 0.334209 5_2:-18444042 None:intergenic
TATTACCCTGGAGTGAGGTT+TGG 0.380467 5_2:-18443614 None:intergenic
TGTAGTTGGTGAAGTGTTGC+GGG 0.387881 5_2:-18443222 None:intergenic
AAGGATTAACTATAACTTAT+GGG 0.387964 5_2:-18443309 None:intergenic
CTCATCATCTTCAGCTTAAG+TGG 0.396808 5_2:+18443487 Msa0761280:CDS
GATCACGGTGGTTTGGGCGG+TGG 0.414427 5_2:-18444035 None:intergenic
ACTTGAGGTGGTGAAAGATC+TGG 0.414803 5_2:-18443412 None:intergenic
ATGTGCGGGCACTTGTACAA+TGG 0.420703 5_2:+18444392 Msa0761280:three_prime_UTR
TTGGTGAAGTGTTGCGGGAA+AGG 0.442491 5_2:-18443217 None:intergenic
TCTTCTCATGTTGCTACCAC+TGG 0.447898 5_2:+18443512 Msa0761280:CDS
CTTGCTCGTGTCAGCATTCG+CGG 0.454035 5_2:+18443982 Msa0761280:CDS
TAGAAAGGGTGGCAAGCAAA+TGG 0.454100 5_2:-18443255 None:intergenic
TAGAATGAAGTGAAATAGAA+AGG 0.457772 5_2:-18443270 None:intergenic
GAGGTTTGGACTTGGAAGGT+GGG 0.459370 5_2:-18443600 None:intergenic
TGAGGTTTGGACTTGGAAGG+TGG 0.462623 5_2:-18443601 None:intergenic
CTTCCTATTGTCTCATGTGC+GGG 0.469223 5_2:+18444378 Msa0761280:three_prime_UTR
CCTGGAGTGAGGTTTGGACT+TGG 0.474511 5_2:-18443608 None:intergenic
CCGTCCCTCGGTTTCCCACT+AGG 0.481711 5_2:+18443448 Msa0761280:CDS
GTGCCCGCACATGAGACAAT+AGG 0.486780 5_2:-18444381 None:intergenic
GTATCAATACAAATAATTAG+TGG 0.492035 5_2:-18444140 None:intergenic
TGTGCGGGCACTTGTACAAT+GGG 0.499636 5_2:+18444393 Msa0761280:three_prime_UTR
CCAAGTCCAAACCTCACTCC+AGG 0.502297 5_2:+18443608 Msa0761280:CDS
GTGTTGCGGGAAAGGATAGT+AGG 0.502510 5_2:-18443209 None:intergenic
AACTTTGGTACCTCATCCCC+TGG 0.505169 5_2:-18443948 None:intergenic
AGAAAGGGTGGCAAGCAAAT+GGG 0.506384 5_2:-18443254 None:intergenic
CAAGGCATGGCTATAAACTT+TGG 0.510814 5_2:-18443963 None:intergenic
TAAGGGATTATGCAGATGGC+AGG 0.513647 5_2:+18444305 Msa0761280:three_prime_UTR
TTGTAGTTGGTGAAGTGTTG+CGG 0.522503 5_2:-18443223 None:intergenic
TCAATCTCTCTCTCATCTTG+AGG 0.522920 5_2:+18443555 Msa0761280:CDS
AGTGTTGATGCCAGGGGATG+AGG 0.523209 5_2:+18443938 Msa0761280:CDS
GAGTGAGGTTTGGACTTGGA+AGG 0.526857 5_2:-18443604 None:intergenic
CATTCATTCTTCTATACACA+TGG 0.526914 5_2:-18444102 None:intergenic
CTTGAGGTGGTGAAAGATCT+GGG 0.527529 5_2:-18443411 None:intergenic
GACACATCTCGACCGTCCCT+CGG 0.527794 5_2:+18443436 Msa0761280:CDS
AGTGTGACAGTGTTGATGCC+AGG 0.532871 5_2:+18443930 Msa0761280:CDS
AGCTGAAGATGATGAGCAAG+AGG 0.534577 5_2:-18443480 None:intergenic
AGAGGAGAGCAGTGCCTAGT+GGG 0.536628 5_2:-18443462 None:intergenic
CTTCTCATGTTGCTACCACT+GGG 0.549662 5_2:+18443513 Msa0761280:CDS
ATACTAAGGGATTATGCAGA+TGG 0.556384 5_2:+18444301 Msa0761280:three_prime_UTR
GTAGTTGAGGAAATTAAGGA+AGG 0.559913 5_2:-18443328 None:intergenic
GGTGATCACGGTGGTTTGGG+CGG 0.561792 5_2:-18444038 None:intergenic
AGGTTTGGACTTGGAAGGTG+GGG 0.562235 5_2:-18443599 None:intergenic
AGAATGAAGTGAAATAGAAA+GGG 0.563062 5_2:-18443269 None:intergenic
ATGAAGTGAAATAGAAAGGG+TGG 0.570081 5_2:-18443266 None:intergenic
GGTCGAGATGTGTCTACTTG+AGG 0.570607 5_2:-18443427 None:intergenic
CGCGAATGCTGACACGAGCA+AGG 0.571573 5_2:-18443981 None:intergenic
GAGTGTATCAACTAGAGAGA+AGG 0.573565 5_2:-18443162 None:intergenic
TGTTGGTGGATAGGAAATGG+AGG 0.574633 5_2:+18443890 Msa0761280:intron
TAGAGAGAAGGAGCAAGTTG+AGG 0.576455 5_2:-18443150 None:intergenic
TCTTCCTATTGTCTCATGTG+CGG 0.578753 5_2:+18444377 Msa0761280:three_prime_UTR
GGGTGGCAAGCAAATGGGAA+AGG 0.586490 5_2:-18443249 None:intergenic
ACATGCTACAATGGTGATCA+CGG 0.589068 5_2:-18444050 None:intergenic
GCGGGAAAGGATAGTAGGCG+TGG 0.589351 5_2:-18443204 None:intergenic
GTTGGTGGATAGGAAATGGA+GGG 0.592995 5_2:+18443891 Msa0761280:intron
TATATGTATGTGTGTAGTTG+AGG 0.597718 5_2:-18443341 None:intergenic
GAAATTATTACCCTGGAGTG+AGG 0.601022 5_2:-18443619 None:intergenic
CAATAATTAACATGCTACAA+TGG 0.602901 5_2:-18444059 None:intergenic
AAGGGATTATGCAGATGGCA+GGG 0.605632 5_2:+18444306 Msa0761280:three_prime_UTR
ATGCTGACACGAGCAAGGCA+TGG 0.607576 5_2:-18443976 None:intergenic
CAGTGCCTAGTGGGAAACCG+AGG 0.610817 5_2:-18443453 None:intergenic
CAAGTCCAAACCTCACTCCA+GGG 0.613631 5_2:+18443609 Msa0761280:CDS
GTGTGACAGTGTTGATGCCA+GGG 0.615539 5_2:+18443931 Msa0761280:CDS
CGGGAAAGGATAGTAGGCGT+GGG 0.622962 5_2:-18443203 None:intergenic
AAGAGGAGAGCAGTGCCTAG+TGG 0.632457 5_2:-18443463 None:intergenic
CGAGATGTGTCTACTTGAGG+TGG 0.649600 5_2:-18443424 None:intergenic
AGTGCCTAGTGGGAAACCGA+GGG 0.658274 5_2:-18443452 None:intergenic
TGGTGGATAGGAAATGGAGG+GGG 0.658677 5_2:+18443893 Msa0761280:intron
CCTAGTGGGAAACCGAGGGA+CGG 0.663157 5_2:-18443448 None:intergenic
TTGGTGGATAGGAAATGGAG+GGG 0.673145 5_2:+18443892 Msa0761280:intron
CAATGTGTTTGTCAGAAACG+TGG 0.674141 5_2:+18444176 Msa0761280:three_prime_UTR
TGTGACAGTGTTGATGCCAG+GGG 0.746992 5_2:+18443932 Msa0761280:CDS
TGCTACAATGGTGATCACGG+TGG 0.756673 5_2:-18444047 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGATTATAATTTTATCT+TGG - chr5_2:18443749-18443768 None:intergenic 10.0%
!!! AATTGATTATAATTTTATCT+TGG - chr5_2:18443749-18443768 None:intergenic 10.0%
!! AATAGATTGTAAAAATACTA+AGG + chr5_2:18444287-18444306 Msa0761280:three_prime_UTR 15.0%
!! ATAGATTGTAAAAATACTAA+GGG + chr5_2:18444288-18444307 Msa0761280:three_prime_UTR 15.0%
!! AATAGATTGTAAAAATACTA+AGG + chr5_2:18444287-18444306 Msa0761280:three_prime_UTR 15.0%
!! ATAGATTGTAAAAATACTAA+GGG + chr5_2:18444288-18444307 Msa0761280:three_prime_UTR 15.0%
!! AAGGATTAACTATAACTTAT+GGG - chr5_2:18443312-18443331 None:intergenic 20.0%
!!! TATATGAGTAGTAGTATTTT+AGG + chr5_2:18443360-18443379 Msa0761280:five_prime_UTR 20.0%
!!! TAGTATTTTAGGAATATCAA+AGG + chr5_2:18443371-18443390 Msa0761280:five_prime_UTR 20.0%
!!! GAATTTACAATTTTGTTTGT+TGG + chr5_2:18443873-18443892 Msa0761280:intron 20.0%
!! GTATCAATACAAATAATTAG+TGG - chr5_2:18444143-18444162 None:intergenic 20.0%
!!! TCGTAATTTTACTGTAATTT+TGG - chr5_2:18444223-18444242 None:intergenic 20.0%
!! AAGGATTAACTATAACTTAT+GGG - chr5_2:18443312-18443331 None:intergenic 20.0%
!!! TATATGAGTAGTAGTATTTT+AGG + chr5_2:18443360-18443379 Msa0761280:five_prime_UTR 20.0%
!!! TAGTATTTTAGGAATATCAA+AGG + chr5_2:18443371-18443390 Msa0761280:five_prime_UTR 20.0%
!!! GAATTTACAATTTTGTTTGT+TGG + chr5_2:18443873-18443892 Msa0761280:intron 20.0%
!! GTATCAATACAAATAATTAG+TGG - chr5_2:18444143-18444162 None:intergenic 20.0%
!!! TCGTAATTTTACTGTAATTT+TGG - chr5_2:18444223-18444242 None:intergenic 20.0%
! AGAATGAAGTGAAATAGAAA+GGG - chr5_2:18443272-18443291 None:intergenic 25.0%
! TAGAATGAAGTGAAATAGAA+AGG - chr5_2:18443273-18443292 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG - chr5_2:18443313-18443332 None:intergenic 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG + chr5_2:18443876-18443895 Msa0761280:intron 25.0%
! CAATAATTAACATGCTACAA+TGG - chr5_2:18444062-18444081 None:intergenic 25.0%
!!! CTGTAATTTTGGTAGAATTA+CGG - chr5_2:18444212-18444231 None:intergenic 25.0%
!! TTACAATCTATTGTTTTCGT+CGG - chr5_2:18444279-18444298 None:intergenic 25.0%
! AGAATGAAGTGAAATAGAAA+GGG - chr5_2:18443272-18443291 None:intergenic 25.0%
! TAGAATGAAGTGAAATAGAA+AGG - chr5_2:18443273-18443292 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG - chr5_2:18443313-18443332 None:intergenic 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG + chr5_2:18443876-18443895 Msa0761280:intron 25.0%
! CAATAATTAACATGCTACAA+TGG - chr5_2:18444062-18444081 None:intergenic 25.0%
!!! CTGTAATTTTGGTAGAATTA+CGG - chr5_2:18444212-18444231 None:intergenic 25.0%
!! TTACAATCTATTGTTTTCGT+CGG - chr5_2:18444279-18444298 None:intergenic 25.0%
TATATGTATGTGTGTAGTTG+AGG - chr5_2:18443344-18443363 None:intergenic 30.0%
AAAGAGTGAAATTATTACCC+TGG - chr5_2:18443629-18443648 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG - chr5_2:18443779-18443798 None:intergenic 30.0%
TTGACGTTGTAAGACAAAAA+AGG - chr5_2:18443799-18443818 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG + chr5_2:18443881-18443900 Msa0761280:intron 30.0%
CATTCATTCTTCTATACACA+TGG - chr5_2:18444105-18444124 None:intergenic 30.0%
! TACAATCTATTGTTTTCGTC+GGG - chr5_2:18444278-18444297 None:intergenic 30.0%
TATATGTATGTGTGTAGTTG+AGG - chr5_2:18443344-18443363 None:intergenic 30.0%
AAAGAGTGAAATTATTACCC+TGG - chr5_2:18443629-18443648 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG - chr5_2:18443779-18443798 None:intergenic 30.0%
TTGACGTTGTAAGACAAAAA+AGG - chr5_2:18443799-18443818 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG + chr5_2:18443881-18443900 Msa0761280:intron 30.0%
CATTCATTCTTCTATACACA+TGG - chr5_2:18444105-18444124 None:intergenic 30.0%
! TACAATCTATTGTTTTCGTC+GGG - chr5_2:18444278-18444297 None:intergenic 30.0%
! ATGGGAAAGGATTTTGTAGT+TGG - chr5_2:18443239-18443258 None:intergenic 35.0%
ATGAAGTGAAATAGAAAGGG+TGG - chr5_2:18443269-18443288 None:intergenic 35.0%
GTAGTTGAGGAAATTAAGGA+AGG - chr5_2:18443331-18443350 None:intergenic 35.0%
GTGTGTAGTTGAGGAAATTA+AGG - chr5_2:18443335-18443354 None:intergenic 35.0%
ATACTAAGGGATTATGCAGA+TGG + chr5_2:18444301-18444320 Msa0761280:three_prime_UTR 35.0%
! ATGGGAAAGGATTTTGTAGT+TGG - chr5_2:18443239-18443258 None:intergenic 35.0%
ATGAAGTGAAATAGAAAGGG+TGG - chr5_2:18443269-18443288 None:intergenic 35.0%
GTAGTTGAGGAAATTAAGGA+AGG - chr5_2:18443331-18443350 None:intergenic 35.0%
GTGTGTAGTTGAGGAAATTA+AGG - chr5_2:18443335-18443354 None:intergenic 35.0%
ATACTAAGGGATTATGCAGA+TGG + chr5_2:18444301-18444320 Msa0761280:three_prime_UTR 35.0%
GAGTGTATCAACTAGAGAGA+AGG - chr5_2:18443165-18443184 None:intergenic 40.0%
!! TTGTAGTTGGTGAAGTGTTG+CGG - chr5_2:18443226-18443245 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG + chr5_2:18443487-18443506 Msa0761280:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG + chr5_2:18443555-18443574 Msa0761280:CDS 40.0%
GAAATTATTACCCTGGAGTG+AGG - chr5_2:18443622-18443641 None:intergenic 40.0%
! GTTTGTTGGTGGATAGGAAA+TGG + chr5_2:18443887-18443906 Msa0761280:intron 40.0%
CAAGGCATGGCTATAAACTT+TGG - chr5_2:18443966-18443985 None:intergenic 40.0%
ACATGCTACAATGGTGATCA+CGG - chr5_2:18444053-18444072 None:intergenic 40.0%
CAATGTGTTTGTCAGAAACG+TGG + chr5_2:18444176-18444195 Msa0761280:three_prime_UTR 40.0%
TCTTCCTATTGTCTCATGTG+CGG + chr5_2:18444377-18444396 Msa0761280:three_prime_UTR 40.0%
GAGTGTATCAACTAGAGAGA+AGG - chr5_2:18443165-18443184 None:intergenic 40.0%
!! TTGTAGTTGGTGAAGTGTTG+CGG - chr5_2:18443226-18443245 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG + chr5_2:18443487-18443506 Msa0761280:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG + chr5_2:18443555-18443574 Msa0761280:CDS 40.0%
GAAATTATTACCCTGGAGTG+AGG - chr5_2:18443622-18443641 None:intergenic 40.0%
! GTTTGTTGGTGGATAGGAAA+TGG + chr5_2:18443887-18443906 Msa0761280:intron 40.0%
CAAGGCATGGCTATAAACTT+TGG - chr5_2:18443966-18443985 None:intergenic 40.0%
ACATGCTACAATGGTGATCA+CGG - chr5_2:18444053-18444072 None:intergenic 40.0%
CAATGTGTTTGTCAGAAACG+TGG + chr5_2:18444176-18444195 Msa0761280:three_prime_UTR 40.0%
TCTTCCTATTGTCTCATGTG+CGG + chr5_2:18444377-18444396 Msa0761280:three_prime_UTR 40.0%
TAGAGAGAAGGAGCAAGTTG+AGG - chr5_2:18443153-18443172 None:intergenic 45.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG - chr5_2:18443225-18443244 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAT+GGG - chr5_2:18443257-18443276 None:intergenic 45.0%
TAGAAAGGGTGGCAAGCAAA+TGG - chr5_2:18443258-18443277 None:intergenic 45.0%
CTTGAGGTGGTGAAAGATCT+GGG - chr5_2:18443414-18443433 None:intergenic 45.0%
ACTTGAGGTGGTGAAAGATC+TGG - chr5_2:18443415-18443434 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG - chr5_2:18443483-18443502 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG + chr5_2:18443512-18443531 Msa0761280:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG + chr5_2:18443513-18443532 Msa0761280:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG - chr5_2:18443531-18443550 None:intergenic 45.0%
TATTACCCTGGAGTGAGGTT+TGG - chr5_2:18443617-18443636 None:intergenic 45.0%
! TGTTGGTGGATAGGAAATGG+AGG + chr5_2:18443890-18443909 Msa0761280:intron 45.0%
! GTTGGTGGATAGGAAATGGA+GGG + chr5_2:18443891-18443910 Msa0761280:intron 45.0%
! TTGGTGGATAGGAAATGGAG+GGG + chr5_2:18443892-18443911 Msa0761280:intron 45.0%
GAAATGGAGGGGGAAAAGAA+GGG + chr5_2:18443903-18443922 Msa0761280:CDS 45.0%
AAATGGAGGGGGAAAAGAAG+GGG + chr5_2:18443904-18443923 Msa0761280:CDS 45.0%
AATGGTGATCACGGTGGTTT+GGG - chr5_2:18444044-18444063 None:intergenic 45.0%
TAAGGGATTATGCAGATGGC+AGG + chr5_2:18444305-18444324 Msa0761280:three_prime_UTR 45.0%
AAGGGATTATGCAGATGGCA+GGG + chr5_2:18444306-18444325 Msa0761280:three_prime_UTR 45.0%
CTTCCTATTGTCTCATGTGC+GGG + chr5_2:18444378-18444397 Msa0761280:three_prime_UTR 45.0%
TAGAGAGAAGGAGCAAGTTG+AGG - chr5_2:18443153-18443172 None:intergenic 45.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG - chr5_2:18443225-18443244 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAT+GGG - chr5_2:18443257-18443276 None:intergenic 45.0%
TAGAAAGGGTGGCAAGCAAA+TGG - chr5_2:18443258-18443277 None:intergenic 45.0%
CTTGAGGTGGTGAAAGATCT+GGG - chr5_2:18443414-18443433 None:intergenic 45.0%
ACTTGAGGTGGTGAAAGATC+TGG - chr5_2:18443415-18443434 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG - chr5_2:18443483-18443502 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG + chr5_2:18443512-18443531 Msa0761280:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG + chr5_2:18443513-18443532 Msa0761280:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG - chr5_2:18443531-18443550 None:intergenic 45.0%
TATTACCCTGGAGTGAGGTT+TGG - chr5_2:18443617-18443636 None:intergenic 45.0%
! TGTTGGTGGATAGGAAATGG+AGG + chr5_2:18443890-18443909 Msa0761280:intron 45.0%
! GTTGGTGGATAGGAAATGGA+GGG + chr5_2:18443891-18443910 Msa0761280:intron 45.0%
! TTGGTGGATAGGAAATGGAG+GGG + chr5_2:18443892-18443911 Msa0761280:intron 45.0%
GAAATGGAGGGGGAAAAGAA+GGG + chr5_2:18443903-18443922 Msa0761280:CDS 45.0%
AAATGGAGGGGGAAAAGAAG+GGG + chr5_2:18443904-18443923 Msa0761280:CDS 45.0%
AATGGTGATCACGGTGGTTT+GGG - chr5_2:18444044-18444063 None:intergenic 45.0%
TAAGGGATTATGCAGATGGC+AGG + chr5_2:18444305-18444324 Msa0761280:three_prime_UTR 45.0%
AAGGGATTATGCAGATGGCA+GGG + chr5_2:18444306-18444325 Msa0761280:three_prime_UTR 45.0%
CTTCCTATTGTCTCATGTGC+GGG + chr5_2:18444378-18444397 Msa0761280:three_prime_UTR 45.0%
!!! ATAATTTGAAATTATAAAAT+TGG + chr5_2:18443691-18443710 Msa0761280:intron 5.0%
!!! ATAATTTGAAATTATAAAAT+TGG + chr5_2:18443691-18443710 Msa0761280:intron 5.0%
GTGTTGCGGGAAAGGATAGT+AGG - chr5_2:18443212-18443231 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG - chr5_2:18443220-18443239 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG - chr5_2:18443427-18443446 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG - chr5_2:18443430-18443449 None:intergenic 50.0%
AGGTTTGGACTTGGAAGGTG+GGG - chr5_2:18443602-18443621 None:intergenic 50.0%
GAGGTTTGGACTTGGAAGGT+GGG - chr5_2:18443603-18443622 None:intergenic 50.0%
TGAGGTTTGGACTTGGAAGG+TGG - chr5_2:18443604-18443623 None:intergenic 50.0%
GAGTGAGGTTTGGACTTGGA+AGG - chr5_2:18443607-18443626 None:intergenic 50.0%
CAAGTCCAAACCTCACTCCA+GGG + chr5_2:18443609-18443628 Msa0761280:CDS 50.0%
! TGGTGGATAGGAAATGGAGG+GGG + chr5_2:18443893-18443912 Msa0761280:intron 50.0%
GGAAATGGAGGGGGAAAAGA+AGG + chr5_2:18443902-18443921 Msa0761280:intron 50.0%
!! AGTGTGACAGTGTTGATGCC+AGG + chr5_2:18443930-18443949 Msa0761280:CDS 50.0%
!! GTGTGACAGTGTTGATGCCA+GGG + chr5_2:18443931-18443950 Msa0761280:CDS 50.0%
!! TGTGACAGTGTTGATGCCAG+GGG + chr5_2:18443932-18443951 Msa0761280:CDS 50.0%
AACTTTGGTACCTCATCCCC+TGG - chr5_2:18443951-18443970 None:intergenic 50.0%
CAATGGTGATCACGGTGGTT+TGG - chr5_2:18444045-18444064 None:intergenic 50.0%
TGCTACAATGGTGATCACGG+TGG - chr5_2:18444050-18444069 None:intergenic 50.0%
ATGTGCGGGCACTTGTACAA+TGG + chr5_2:18444392-18444411 Msa0761280:three_prime_UTR 50.0%
TGTGCGGGCACTTGTACAAT+GGG + chr5_2:18444393-18444412 Msa0761280:three_prime_UTR 50.0%
GTGTTGCGGGAAAGGATAGT+AGG - chr5_2:18443212-18443231 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG - chr5_2:18443220-18443239 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG - chr5_2:18443427-18443446 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG - chr5_2:18443430-18443449 None:intergenic 50.0%
AGGTTTGGACTTGGAAGGTG+GGG - chr5_2:18443602-18443621 None:intergenic 50.0%
GAGGTTTGGACTTGGAAGGT+GGG - chr5_2:18443603-18443622 None:intergenic 50.0%
TGAGGTTTGGACTTGGAAGG+TGG - chr5_2:18443604-18443623 None:intergenic 50.0%
GAGTGAGGTTTGGACTTGGA+AGG - chr5_2:18443607-18443626 None:intergenic 50.0%
CAAGTCCAAACCTCACTCCA+GGG + chr5_2:18443609-18443628 Msa0761280:CDS 50.0%
! TGGTGGATAGGAAATGGAGG+GGG + chr5_2:18443893-18443912 Msa0761280:intron 50.0%
GGAAATGGAGGGGGAAAAGA+AGG + chr5_2:18443902-18443921 Msa0761280:intron 50.0%
!! AGTGTGACAGTGTTGATGCC+AGG + chr5_2:18443930-18443949 Msa0761280:CDS 50.0%
!! GTGTGACAGTGTTGATGCCA+GGG + chr5_2:18443931-18443950 Msa0761280:CDS 50.0%
!! TGTGACAGTGTTGATGCCAG+GGG + chr5_2:18443932-18443951 Msa0761280:CDS 50.0%
AACTTTGGTACCTCATCCCC+TGG - chr5_2:18443951-18443970 None:intergenic 50.0%
CAATGGTGATCACGGTGGTT+TGG - chr5_2:18444045-18444064 None:intergenic 50.0%
TGCTACAATGGTGATCACGG+TGG - chr5_2:18444050-18444069 None:intergenic 50.0%
ATGTGCGGGCACTTGTACAA+TGG + chr5_2:18444392-18444411 Msa0761280:three_prime_UTR 50.0%
TGTGCGGGCACTTGTACAAT+GGG + chr5_2:18444393-18444412 Msa0761280:three_prime_UTR 50.0%
CGGGAAAGGATAGTAGGCGT+GGG - chr5_2:18443206-18443225 None:intergenic 55.0%
GGGTGGCAAGCAAATGGGAA+AGG - chr5_2:18443252-18443271 None:intergenic 55.0%
AGTGCCTAGTGGGAAACCGA+GGG - chr5_2:18443455-18443474 None:intergenic 55.0%
AGAGGAGAGCAGTGCCTAGT+GGG - chr5_2:18443465-18443484 None:intergenic 55.0%
AAGAGGAGAGCAGTGCCTAG+TGG - chr5_2:18443466-18443485 None:intergenic 55.0%
GGACTTGGAAGGTGGGGATT+GGG - chr5_2:18443596-18443615 None:intergenic 55.0%
TGGACTTGGAAGGTGGGGAT+TGG - chr5_2:18443597-18443616 None:intergenic 55.0%
CCTGGAGTGAGGTTTGGACT+TGG - chr5_2:18443611-18443630 None:intergenic 55.0%
CCAAGTCCAAACCTCACTCC+AGG + chr5_2:18443608-18443627 Msa0761280:CDS 55.0%
!! AGTGTTGATGCCAGGGGATG+AGG + chr5_2:18443938-18443957 Msa0761280:CDS 55.0%
ATGCTGACACGAGCAAGGCA+TGG - chr5_2:18443979-18443998 None:intergenic 55.0%
CTTGCTCGTGTCAGCATTCG+CGG + chr5_2:18443982-18444001 Msa0761280:CDS 55.0%
GTGCCCGCACATGAGACAAT+AGG - chr5_2:18444384-18444403 None:intergenic 55.0%
CGGGAAAGGATAGTAGGCGT+GGG - chr5_2:18443206-18443225 None:intergenic 55.0%
GGGTGGCAAGCAAATGGGAA+AGG - chr5_2:18443252-18443271 None:intergenic 55.0%
AGTGCCTAGTGGGAAACCGA+GGG - chr5_2:18443455-18443474 None:intergenic 55.0%
AGAGGAGAGCAGTGCCTAGT+GGG - chr5_2:18443465-18443484 None:intergenic 55.0%
AAGAGGAGAGCAGTGCCTAG+TGG - chr5_2:18443466-18443485 None:intergenic 55.0%
GGACTTGGAAGGTGGGGATT+GGG - chr5_2:18443596-18443615 None:intergenic 55.0%
TGGACTTGGAAGGTGGGGAT+TGG - chr5_2:18443597-18443616 None:intergenic 55.0%
CCTGGAGTGAGGTTTGGACT+TGG - chr5_2:18443611-18443630 None:intergenic 55.0%
CCAAGTCCAAACCTCACTCC+AGG + chr5_2:18443608-18443627 Msa0761280:CDS 55.0%
!! AGTGTTGATGCCAGGGGATG+AGG + chr5_2:18443938-18443957 Msa0761280:CDS 55.0%
ATGCTGACACGAGCAAGGCA+TGG - chr5_2:18443979-18443998 None:intergenic 55.0%
CTTGCTCGTGTCAGCATTCG+CGG + chr5_2:18443982-18444001 Msa0761280:CDS 55.0%
GTGCCCGCACATGAGACAAT+AGG - chr5_2:18444384-18444403 None:intergenic 55.0%
GCGGGAAAGGATAGTAGGCG+TGG - chr5_2:18443207-18443226 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG + chr5_2:18443436-18443455 Msa0761280:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG - chr5_2:18443451-18443470 None:intergenic 60.0%
CAGTGCCTAGTGGGAAACCG+AGG - chr5_2:18443456-18443475 None:intergenic 60.0%
CGCGAATGCTGACACGAGCA+AGG - chr5_2:18443984-18444003 None:intergenic 60.0%
! GGTGATCACGGTGGTTTGGG+CGG - chr5_2:18444041-18444060 None:intergenic 60.0%
GCGGGAAAGGATAGTAGGCG+TGG - chr5_2:18443207-18443226 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG + chr5_2:18443436-18443455 Msa0761280:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG - chr5_2:18443451-18443470 None:intergenic 60.0%
CAGTGCCTAGTGGGAAACCG+AGG - chr5_2:18443456-18443475 None:intergenic 60.0%
CGCGAATGCTGACACGAGCA+AGG - chr5_2:18443984-18444003 None:intergenic 60.0%
! GGTGATCACGGTGGTTTGGG+CGG - chr5_2:18444041-18444060 None:intergenic 60.0%
CCGTCCCTCGGTTTCCCACT+AGG + chr5_2:18443448-18443467 Msa0761280:CDS 65.0%
! GATCACGGTGGTTTGGGCGG+TGG - chr5_2:18444038-18444057 None:intergenic 65.0%
CCGTCCCTCGGTTTCCCACT+AGG + chr5_2:18443448-18443467 Msa0761280:CDS 65.0%
! GATCACGGTGGTTTGGGCGG+TGG - chr5_2:18444038-18444057 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 18443119 18444420 18443119 ID=Msa0761280;Name=Msa0761280
chr5_2 mRNA 18443119 18444420 18443119 ID=Msa0761280-mRNA-1;Parent=Msa0761280;Name=Msa0761280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=278|1|1|1|1|1|2|365|128
chr5_2 exon 18443119 18443630 18443119 ID=Msa0761280-mRNA-1:exon:8007;Parent=Msa0761280-mRNA-1
chr5_2 exon 18443903 18444420 18443903 ID=Msa0761280-mRNA-1:exon:8008;Parent=Msa0761280-mRNA-1
chr5_2 five_prime_UTR 18443119 18443396 18443119 ID=Msa0761280-mRNA-1:five_prime_utr;Parent=Msa0761280-mRNA-1
chr5_2 CDS 18443397 18443630 18443397 ID=Msa0761280-mRNA-1:cds;Parent=Msa0761280-mRNA-1
chr5_2 CDS 18443903 18444055 18443903 ID=Msa0761280-mRNA-1:cds;Parent=Msa0761280-mRNA-1
chr5_2 three_prime_UTR 18444056 18444420 18444056 ID=Msa0761280-mRNA-1:three_prime_utr;Parent=Msa0761280-mRNA-1
Gene Sequence

>Msa0761280

ATGATGATGAAAAGCCCAGATCTTTCACCACCTCAAGTAGACACATCTCGACCGTCCCTCGGTTTCCCACTAGGCACTGCTCTCCTCTTGCTCATCATCTTCAGCTTAAGTGGTATCTTCTCATGTTGCTACCACTGGGAAAAGTTTCGTTCACTTCATCAATCTCTCTCTCATCTTGAGGCTGCACAAGCACATACCCAATCCCCACCTTCCAAGTCCAAACCTCACTCCAGGGAAATGGAGGGGGAAAAGAAGGGGCAGAGTGTGACAGTGTTGATGCCAGGGGATGAGGTACCAAAGTTTATAGCCATGCCTTGCTCGTGTCAGCATTCGCGGCTAGAAGAGATTATTGTCAACATCGAGAAGCCACCGCCCAAACCACCGTGA
Protein sequence

>Msa0761280

MMMKSPDLSPPQVDTSRPSLGFPLGTALLLLIIFSLSGIFSCCYHWEKFRSLHQSLSHLEAAQAHTQSPPSKSKPHSREMEGEKKGQSVTVLMPGDEVPKFIAMPCSCQHSRLEEIIVNIEKPPPKPP*