Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762280 | G7JXR4 | 93.220 | 177 | 12 | 0 | 1 | 177 | 1 | 177 | 1.40e-116 | 337 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0762280 | Msa0983970 | 0.809372 | 1.955285e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762280 | MtrunA17_Chr5g0412491 | 92.814 | 167 | 12 | 0 | 11 | 177 | 1 | 167 | 1.94e-110 | 311 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762280 | AT1G26290.1 | 41.129 | 124 | 64 | 3 | 1 | 123 | 1 | 116 | 3.16e-16 | 72.0 |
Find 38 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTACTATGCCTATTATTT+TGG | 0.035699 | 5_2:+20214114 | Msa0762280:three_prime_UTR |
| ATTAAATATATAATTATATT+TGG | 0.157980 | 5_2:-20213412 | None:intergenic |
| AGATTTAGAAGAGGAGATTA+AGG | 0.230236 | 5_2:+20213640 | Msa0762280:CDS |
| TAACAAAGAAGCAAATAAAA+AGG | 0.238986 | 5_2:+20213832 | Msa0762280:CDS |
| AAGAAGTGAAGAAGATTGAT+TGG | 0.303832 | 5_2:+20213897 | Msa0762280:CDS |
| GGTTTAAAGCTCAATTTCTT+TGG | 0.309820 | 5_2:+20214054 | Msa0762280:exon |
| AAAATTCATCCCAAAATAAT+AGG | 0.313572 | 5_2:-20214124 | None:intergenic |
| TTCAACTCCTCTTCTGAAAT+AGG | 0.330003 | 5_2:-20213584 | None:intergenic |
| TATGATCCTTTCACAGGAAT+AGG | 0.366159 | 5_2:-20213947 | None:intergenic |
| TATATTTAATTGAAATATCA+TGG | 0.373913 | 5_2:+20213425 | Msa0762280:exon |
| ATGATCCTTTCACAGGAATA+GGG | 0.396452 | 5_2:-20213946 | None:intergenic |
| ATCTTGCTCGTCGATTGCTT+CGG | 0.414612 | 5_2:+20213675 | Msa0762280:CDS |
| AACTTTATTCATATCCGATC+AGG | 0.435022 | 5_2:-20214147 | None:intergenic |
| AGGAATTAAAATTGAAATCA+AGG | 0.437360 | 5_2:+20213805 | Msa0762280:CDS |
| TTGATTGGGAGAAGAGTTTG+AGG | 0.445900 | 5_2:+20213912 | Msa0762280:CDS |
| GAAGGAGAGAGATGCAAAAG+AGG | 0.478321 | 5_2:+20213712 | Msa0762280:CDS |
| TTAGAAGAGGAGATTAAGGA+AGG | 0.483543 | 5_2:+20213644 | Msa0762280:CDS |
| GGTGATTATAAGAATGAGAA+TGG | 0.483752 | 5_2:+20213778 | Msa0762280:CDS |
| AGAAGTGAAGAAGATTGATT+GGG | 0.484700 | 5_2:+20213898 | Msa0762280:CDS |
| AAAGGATAAAATAATGATGA+GGG | 0.497614 | 5_2:+20213985 | Msa0762280:CDS |
| TGATTGGGAGAAGAGTTTGA+GGG | 0.505362 | 5_2:+20213913 | Msa0762280:CDS |
| TTAGCATATGATCCTTTCAC+AGG | 0.512165 | 5_2:-20213953 | None:intergenic |
| ATATGCTAAATCAAAGCAAA+AGG | 0.512701 | 5_2:+20213967 | Msa0762280:CDS |
| TGAAATATCATGGATCATCA+TGG | 0.515805 | 5_2:+20213435 | Msa0762280:exon |
| AGAAGCTACTTCAGTTGTTG+TGG | 0.517257 | 5_2:+20214029 | Msa0762280:CDS |
| ATTATAAGAATGAGAATGGA+AGG | 0.530576 | 5_2:+20213782 | Msa0762280:CDS |
| GCTACTTCAGTTGTTGTGGA+AGG | 0.547620 | 5_2:+20214033 | Msa0762280:CDS |
| TGAGGTTCCTATTTCAGAAG+AGG | 0.548928 | 5_2:+20213577 | Msa0762280:CDS |
| AAAAGGATAAAATAATGATG+AGG | 0.564917 | 5_2:+20213984 | Msa0762280:CDS |
| CAAAATTAGAGCACTTTCTG+AGG | 0.566466 | 5_2:+20213757 | Msa0762280:CDS |
| GATTGAAAGAGATTTAGAAG+AGG | 0.567737 | 5_2:+20213631 | Msa0762280:CDS |
| GAGGGGAAAGAATGCAAGTG+AGG | 0.574468 | 5_2:+20214003 | Msa0762280:CDS |
| TCAAGCCCTATTCCTGTGAA+AGG | 0.575760 | 5_2:+20213941 | Msa0762280:CDS |
| TGGGAGAAGAGTTTGAGGGC+AGG | 0.600710 | 5_2:+20213917 | Msa0762280:CDS |
| CATCATGGAATTTACACCAA+AGG | 0.610685 | 5_2:+20213450 | Msa0762280:CDS |
| ATAAGAATGAGAATGGAAGG+AGG | 0.629401 | 5_2:+20213785 | Msa0762280:CDS |
| AAGGATAAAATAATGATGAG+GGG | 0.646560 | 5_2:+20213986 | Msa0762280:CDS |
| AATTAAAATTGAAATCAAGG+AGG | 0.676534 | 5_2:+20213808 | Msa0762280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTTAATTGAAATATCA+TGG | + | chr5_2:20213425-20213444 | Msa0762280:exon | 10.0% |
| !! | TATATTTAATTGAAATATCA+TGG | + | chr5_2:20213425-20213444 | Msa0762280:exon | 10.0% |
| !!! | ATTTTCTTGCTTTTTTTAAT+TGG | + | chr5_2:20214081-20214100 | Msa0762280:three_prime_UTR | 15.0% |
| !!! | ATTTTCTTGCTTTTTTTAAT+TGG | + | chr5_2:20214081-20214100 | Msa0762280:three_prime_UTR | 15.0% |
| !! | AGGAATTAAAATTGAAATCA+AGG | + | chr5_2:20213805-20213824 | Msa0762280:CDS | 20.0% |
| !! | AATTAAAATTGAAATCAAGG+AGG | + | chr5_2:20213808-20213827 | Msa0762280:CDS | 20.0% |
| !! | TAACAAAGAAGCAAATAAAA+AGG | + | chr5_2:20213832-20213851 | Msa0762280:CDS | 20.0% |
| !! | AACAAAGAAGCAAATAAAAA+GGG | + | chr5_2:20213833-20213852 | Msa0762280:CDS | 20.0% |
| !!! | ACTTCTTTAAAACAGTTTTT+GGG | - | chr5_2:20213884-20213903 | None:intergenic | 20.0% |
| !! | AAAAGGATAAAATAATGATG+AGG | + | chr5_2:20213984-20214003 | Msa0762280:CDS | 20.0% |
| !! | AAAGGATAAAATAATGATGA+GGG | + | chr5_2:20213985-20214004 | Msa0762280:CDS | 20.0% |
| !! | AAAATTCATCCCAAAATAAT+AGG | - | chr5_2:20214127-20214146 | None:intergenic | 20.0% |
| !! | AGGAATTAAAATTGAAATCA+AGG | + | chr5_2:20213805-20213824 | Msa0762280:CDS | 20.0% |
| !! | AATTAAAATTGAAATCAAGG+AGG | + | chr5_2:20213808-20213827 | Msa0762280:CDS | 20.0% |
| !! | TAACAAAGAAGCAAATAAAA+AGG | + | chr5_2:20213832-20213851 | Msa0762280:CDS | 20.0% |
| !! | AACAAAGAAGCAAATAAAAA+GGG | + | chr5_2:20213833-20213852 | Msa0762280:CDS | 20.0% |
| !!! | ACTTCTTTAAAACAGTTTTT+GGG | - | chr5_2:20213884-20213903 | None:intergenic | 20.0% |
| !! | AAAAGGATAAAATAATGATG+AGG | + | chr5_2:20213984-20214003 | Msa0762280:CDS | 20.0% |
| !! | AAAGGATAAAATAATGATGA+GGG | + | chr5_2:20213985-20214004 | Msa0762280:CDS | 20.0% |
| !! | AAAATTCATCCCAAAATAAT+AGG | - | chr5_2:20214127-20214146 | None:intergenic | 20.0% |
| ! | GTCAAATAAAAATGTTCTTG+AGG | + | chr5_2:20213559-20213578 | Msa0762280:CDS | 25.0% |
| ! | ATTATAAGAATGAGAATGGA+AGG | + | chr5_2:20213782-20213801 | Msa0762280:CDS | 25.0% |
| !!! | CACTTCTTTAAAACAGTTTT+TGG | - | chr5_2:20213885-20213904 | None:intergenic | 25.0% |
| ! | ATATGCTAAATCAAAGCAAA+AGG | + | chr5_2:20213967-20213986 | Msa0762280:CDS | 25.0% |
| ! | AAGGATAAAATAATGATGAG+GGG | + | chr5_2:20213986-20214005 | Msa0762280:CDS | 25.0% |
| !! | TCTTACTATGCCTATTATTT+TGG | + | chr5_2:20214114-20214133 | Msa0762280:three_prime_UTR | 25.0% |
| !! | CTTACTATGCCTATTATTTT+GGG | + | chr5_2:20214115-20214134 | Msa0762280:three_prime_UTR | 25.0% |
| ! | GTCAAATAAAAATGTTCTTG+AGG | + | chr5_2:20213559-20213578 | Msa0762280:CDS | 25.0% |
| ! | ATTATAAGAATGAGAATGGA+AGG | + | chr5_2:20213782-20213801 | Msa0762280:CDS | 25.0% |
| !!! | CACTTCTTTAAAACAGTTTT+TGG | - | chr5_2:20213885-20213904 | None:intergenic | 25.0% |
| ! | ATATGCTAAATCAAAGCAAA+AGG | + | chr5_2:20213967-20213986 | Msa0762280:CDS | 25.0% |
| ! | AAGGATAAAATAATGATGAG+GGG | + | chr5_2:20213986-20214005 | Msa0762280:CDS | 25.0% |
| !! | TCTTACTATGCCTATTATTT+TGG | + | chr5_2:20214114-20214133 | Msa0762280:three_prime_UTR | 25.0% |
| !! | CTTACTATGCCTATTATTTT+GGG | + | chr5_2:20214115-20214134 | Msa0762280:three_prime_UTR | 25.0% |
| TGAAATATCATGGATCATCA+TGG | + | chr5_2:20213435-20213454 | Msa0762280:exon | 30.0% | |
| CTAGAATTTCATTTCACCTT+TGG | - | chr5_2:20213469-20213488 | None:intergenic | 30.0% | |
| GATTGAAAGAGATTTAGAAG+AGG | + | chr5_2:20213631-20213650 | Msa0762280:CDS | 30.0% | |
| AGATTTAGAAGAGGAGATTA+AGG | + | chr5_2:20213640-20213659 | Msa0762280:CDS | 30.0% | |
| GGTGATTATAAGAATGAGAA+TGG | + | chr5_2:20213778-20213797 | Msa0762280:CDS | 30.0% | |
| !!! | CAGTTTTTGGGTCTAAAATT+TGG | - | chr5_2:20213872-20213891 | None:intergenic | 30.0% |
| AAGAAGTGAAGAAGATTGAT+TGG | + | chr5_2:20213897-20213916 | Msa0762280:CDS | 30.0% | |
| AGAAGTGAAGAAGATTGATT+GGG | + | chr5_2:20213898-20213917 | Msa0762280:CDS | 30.0% | |
| ! | GGTTTAAAGCTCAATTTCTT+TGG | + | chr5_2:20214054-20214073 | Msa0762280:exon | 30.0% |
| AACTTTATTCATATCCGATC+AGG | - | chr5_2:20214150-20214169 | None:intergenic | 30.0% | |
| TGAAATATCATGGATCATCA+TGG | + | chr5_2:20213435-20213454 | Msa0762280:exon | 30.0% | |
| CTAGAATTTCATTTCACCTT+TGG | - | chr5_2:20213469-20213488 | None:intergenic | 30.0% | |
| GATTGAAAGAGATTTAGAAG+AGG | + | chr5_2:20213631-20213650 | Msa0762280:CDS | 30.0% | |
| AGATTTAGAAGAGGAGATTA+AGG | + | chr5_2:20213640-20213659 | Msa0762280:CDS | 30.0% | |
| GGTGATTATAAGAATGAGAA+TGG | + | chr5_2:20213778-20213797 | Msa0762280:CDS | 30.0% | |
| !!! | CAGTTTTTGGGTCTAAAATT+TGG | - | chr5_2:20213872-20213891 | None:intergenic | 30.0% |
| AAGAAGTGAAGAAGATTGAT+TGG | + | chr5_2:20213897-20213916 | Msa0762280:CDS | 30.0% | |
| AGAAGTGAAGAAGATTGATT+GGG | + | chr5_2:20213898-20213917 | Msa0762280:CDS | 30.0% | |
| ! | GGTTTAAAGCTCAATTTCTT+TGG | + | chr5_2:20214054-20214073 | Msa0762280:exon | 30.0% |
| AACTTTATTCATATCCGATC+AGG | - | chr5_2:20214150-20214169 | None:intergenic | 30.0% | |
| CATCATGGAATTTACACCAA+AGG | + | chr5_2:20213450-20213469 | Msa0762280:CDS | 35.0% | |
| !! | TTCAACTCCTCTTCTGAAAT+AGG | - | chr5_2:20213587-20213606 | None:intergenic | 35.0% |
| TTAGAAGAGGAGATTAAGGA+AGG | + | chr5_2:20213644-20213663 | Msa0762280:CDS | 35.0% | |
| ! | CAAAATTAGAGCACTTTCTG+AGG | + | chr5_2:20213757-20213776 | Msa0762280:CDS | 35.0% |
| ATAAGAATGAGAATGGAAGG+AGG | + | chr5_2:20213785-20213804 | Msa0762280:CDS | 35.0% | |
| ATGATCCTTTCACAGGAATA+GGG | - | chr5_2:20213949-20213968 | None:intergenic | 35.0% | |
| TATGATCCTTTCACAGGAAT+AGG | - | chr5_2:20213950-20213969 | None:intergenic | 35.0% | |
| TTAGCATATGATCCTTTCAC+AGG | - | chr5_2:20213956-20213975 | None:intergenic | 35.0% | |
| ! | TTGGGATGAATTTTCCTGAT+CGG | + | chr5_2:20214133-20214152 | Msa0762280:three_prime_UTR | 35.0% |
| CATCATGGAATTTACACCAA+AGG | + | chr5_2:20213450-20213469 | Msa0762280:CDS | 35.0% | |
| !! | TTCAACTCCTCTTCTGAAAT+AGG | - | chr5_2:20213587-20213606 | None:intergenic | 35.0% |
| TTAGAAGAGGAGATTAAGGA+AGG | + | chr5_2:20213644-20213663 | Msa0762280:CDS | 35.0% | |
| ! | CAAAATTAGAGCACTTTCTG+AGG | + | chr5_2:20213757-20213776 | Msa0762280:CDS | 35.0% |
| ATAAGAATGAGAATGGAAGG+AGG | + | chr5_2:20213785-20213804 | Msa0762280:CDS | 35.0% | |
| ATGATCCTTTCACAGGAATA+GGG | - | chr5_2:20213949-20213968 | None:intergenic | 35.0% | |
| TATGATCCTTTCACAGGAAT+AGG | - | chr5_2:20213950-20213969 | None:intergenic | 35.0% | |
| TTAGCATATGATCCTTTCAC+AGG | - | chr5_2:20213956-20213975 | None:intergenic | 35.0% | |
| ! | TTGGGATGAATTTTCCTGAT+CGG | + | chr5_2:20214133-20214152 | Msa0762280:three_prime_UTR | 35.0% |
| TGAGGTTCCTATTTCAGAAG+AGG | + | chr5_2:20213577-20213596 | Msa0762280:CDS | 40.0% | |
| TTGATTGGGAGAAGAGTTTG+AGG | + | chr5_2:20213912-20213931 | Msa0762280:CDS | 40.0% | |
| TGATTGGGAGAAGAGTTTGA+GGG | + | chr5_2:20213913-20213932 | Msa0762280:CDS | 40.0% | |
| AGAAGCTACTTCAGTTGTTG+TGG | + | chr5_2:20214029-20214048 | Msa0762280:CDS | 40.0% | |
| TGAGGTTCCTATTTCAGAAG+AGG | + | chr5_2:20213577-20213596 | Msa0762280:CDS | 40.0% | |
| TTGATTGGGAGAAGAGTTTG+AGG | + | chr5_2:20213912-20213931 | Msa0762280:CDS | 40.0% | |
| TGATTGGGAGAAGAGTTTGA+GGG | + | chr5_2:20213913-20213932 | Msa0762280:CDS | 40.0% | |
| AGAAGCTACTTCAGTTGTTG+TGG | + | chr5_2:20214029-20214048 | Msa0762280:CDS | 40.0% | |
| ! | ATCTTGCTCGTCGATTGCTT+CGG | + | chr5_2:20213675-20213694 | Msa0762280:CDS | 45.0% |
| !! | TGCTTCGGATTTTTCAGCAG+AGG | + | chr5_2:20213690-20213709 | Msa0762280:CDS | 45.0% |
| !! | TCGGATTTTTCAGCAGAGGA+AGG | + | chr5_2:20213694-20213713 | Msa0762280:CDS | 45.0% |
| GAAGGAGAGAGATGCAAAAG+AGG | + | chr5_2:20213712-20213731 | Msa0762280:CDS | 45.0% | |
| TCAAGCCCTATTCCTGTGAA+AGG | + | chr5_2:20213941-20213960 | Msa0762280:CDS | 45.0% | |
| GCTACTTCAGTTGTTGTGGA+AGG | + | chr5_2:20214033-20214052 | Msa0762280:CDS | 45.0% | |
| ! | ATCTTGCTCGTCGATTGCTT+CGG | + | chr5_2:20213675-20213694 | Msa0762280:CDS | 45.0% |
| !! | TGCTTCGGATTTTTCAGCAG+AGG | + | chr5_2:20213690-20213709 | Msa0762280:CDS | 45.0% |
| !! | TCGGATTTTTCAGCAGAGGA+AGG | + | chr5_2:20213694-20213713 | Msa0762280:CDS | 45.0% |
| GAAGGAGAGAGATGCAAAAG+AGG | + | chr5_2:20213712-20213731 | Msa0762280:CDS | 45.0% | |
| TCAAGCCCTATTCCTGTGAA+AGG | + | chr5_2:20213941-20213960 | Msa0762280:CDS | 45.0% | |
| GCTACTTCAGTTGTTGTGGA+AGG | + | chr5_2:20214033-20214052 | Msa0762280:CDS | 45.0% | |
| GAGGGGAAAGAATGCAAGTG+AGG | + | chr5_2:20214003-20214022 | Msa0762280:CDS | 50.0% | |
| GAGGGGAAAGAATGCAAGTG+AGG | + | chr5_2:20214003-20214022 | Msa0762280:CDS | 50.0% | |
| TGGGAGAAGAGTTTGAGGGC+AGG | + | chr5_2:20213917-20213936 | Msa0762280:CDS | 55.0% | |
| TGGGAGAAGAGTTTGAGGGC+AGG | + | chr5_2:20213917-20213936 | Msa0762280:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 20213414 | 20214182 | 20213414 | ID=Msa0762280;Name=Msa0762280 |
| chr5_2 | mRNA | 20213414 | 20214182 | 20213414 | ID=Msa0762280-mRNA-1;Parent=Msa0762280;Name=Msa0762280-mRNA-1;_AED=0.01;_eAED=0.01;_QI=30|1|1|1|1|1|2|122|177 |
| chr5_2 | exon | 20213414 | 20213471 | 20213414 | ID=Msa0762280-mRNA-1:exon:8554;Parent=Msa0762280-mRNA-1 |
| chr5_2 | exon | 20213555 | 20214182 | 20213555 | ID=Msa0762280-mRNA-1:exon:8555;Parent=Msa0762280-mRNA-1 |
| chr5_2 | five_prime_UTR | 20213414 | 20213443 | 20213414 | ID=Msa0762280-mRNA-1:five_prime_utr;Parent=Msa0762280-mRNA-1 |
| chr5_2 | CDS | 20213444 | 20213471 | 20213444 | ID=Msa0762280-mRNA-1:cds;Parent=Msa0762280-mRNA-1 |
| chr5_2 | CDS | 20213555 | 20214060 | 20213555 | ID=Msa0762280-mRNA-1:cds;Parent=Msa0762280-mRNA-1 |
| chr5_2 | three_prime_UTR | 20214061 | 20214182 | 20214061 | ID=Msa0762280-mRNA-1:three_prime_utr;Parent=Msa0762280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
