AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0764500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0764500 MtrunA17_Chr5g0414931 87.755 245 21 2 1 244 1 237 1.39e-126 358
Msa0764500 MtrunA17_Chr1g0170741 63.525 244 43 5 1 244 1 198 1.23e-81 243
Msa0764500 MtrunA17_Chr4g0059571 65.563 151 40 2 15 165 25 163 3.39e-59 186
Msa0764500 MtrunA17_Chr7g0256551 54.545 165 62 3 15 174 8 164 1.61e-54 175
Msa0764500 MtrunA17_Chr3g0118211 61.379 145 44 2 15 159 22 154 1.18e-53 171
Msa0764500 MtrunA17_Chr8g0352871 53.846 156 58 3 15 165 8 154 2.53e-47 154
Msa0764500 MtrunA17_Chr3g0082861 52.632 152 59 2 15 161 3 146 4.21e-47 154
Msa0764500 MtrunA17_Chr3g0128691 51.408 142 53 3 17 155 19 147 7.04e-44 145
Msa0764500 MtrunA17_Chr3g0124861 45.625 160 64 3 10 155 16 166 2.55e-41 141
Msa0764500 MtrunA17_Chr4g0021731 61.039 77 24 3 15 90 10 81 1.30e-21 85.9
Msa0764500 MtrunA17_Chr4g0030271 32.168 143 76 3 16 158 89 210 2.14e-14 72.4
Msa0764500 MtrunA17_Chr3g0127941 27.363 201 111 7 16 202 56 235 4.07e-14 71.2
Msa0764500 MtrunA17_Chr8g0345901 27.891 147 85 2 14 160 34 159 7.30e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0764500 AT1G59940.1 52.542 236 65 6 11 236 28 226 3.31e-67 207
Msa0764500 AT1G59940.2 52.542 236 65 6 11 236 120 318 7.23e-66 207
Msa0764500 AT1G10470.3 50.192 261 63 9 11 240 15 239 1.30e-63 199
Msa0764500 AT1G10470.1 50.192 261 63 9 11 240 29 253 2.72e-63 198
Msa0764500 AT3G48100.1 64.626 147 41 3 16 162 25 160 6.63e-58 182
Msa0764500 AT5G62920.1 68.116 138 30 3 16 152 25 149 2.72e-56 178
Msa0764500 AT1G19050.1 57.862 159 55 2 1 159 9 155 1.14e-53 172
Msa0764500 AT3G57040.2 52.514 179 66 4 15 187 8 173 2.88e-53 172
Msa0764500 AT3G57040.1 52.514 179 66 4 15 187 8 173 2.88e-53 172
Msa0764500 AT1G74890.1 62.319 138 40 2 15 152 17 142 1.06e-51 167
Msa0764500 AT2G41310.1 56.209 153 55 3 17 165 10 154 2.74e-50 164
Msa0764500 AT3G56380.1 54.225 142 54 2 14 155 18 148 2.30e-49 159
Msa0764500 AT3G56380.2 54.225 142 55 2 14 155 18 149 4.35e-48 156
Msa0764500 AT2G40670.1 53.846 143 55 3 14 155 27 159 3.29e-47 154
Msa0764500 AT2G40670.2 53.846 143 56 3 14 155 27 160 3.56e-46 152
Msa0764500 AT1G10470.2 40.120 167 45 5 105 240 35 177 3.48e-21 87.8
Msa0764500 AT2G07440.1 55.000 60 27 0 106 165 9 68 6.34e-15 70.1
Msa0764500 AT5G24470.1 28.276 145 81 5 16 160 52 173 1.46e-11 64.3
Msa0764500 AT2G46790.3 27.972 143 82 3 16 158 37 158 2.80e-11 63.2
Msa0764500 AT2G46790.1 27.972 143 82 3 16 158 37 158 3.25e-11 63.2

Find 68 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCACTCCTCAATATTTA+AGG 0.117042 5_2:+24622510 Msa0764500:five_prime_UTR
AATAAGAGCACTACAATTTC+TGG 0.202396 5_2:+24622935 Msa0764500:CDS
TTCATCTTTAGTAAAGAAAA+AGG 0.245307 5_2:-24624249 None:intergenic
TTCTCAAGAGGTTCATGTTT+TGG 0.250601 5_2:+24622648 Msa0764500:CDS
ATCCCCTTTATCTTTCTCTT+TGG 0.281133 5_2:+24622554 Msa0764500:five_prime_UTR
ACTGGCACCGAATGACTCTT+TGG 0.303564 5_2:+24623953 Msa0764500:exon
CAAAAGGGCATAATCTTTAT+AGG 0.307190 5_2:-24624289 None:intergenic
GAAACCAAAGAGAAAGATAA+AGG 0.307874 5_2:-24622558 None:intergenic
GCAATTACATACCCGTTGTT+TGG 0.343337 5_2:+24624059 Msa0764500:three_prime_UTR
GTTTCCTTAGCCTGGTTTGA+AGG 0.364670 5_2:+24623090 Msa0764500:intron
CTCAAAATTTCTTCTTGTAA+AGG 0.371492 5_2:+24622715 Msa0764500:CDS
GTTCTTAATGATCTTATCCA+TGG 0.386429 5_2:+24624105 Msa0764500:three_prime_UTR
GAGGTGAACAAGTAAATGTT+TGG 0.399962 5_2:-24624020 None:intergenic
ATCTGATGTAAAACGTTTGA+AGG 0.402266 5_2:+24623667 Msa0764500:CDS
TTGTGATAACCAGTCATTCC+TGG 0.408381 5_2:-24623142 None:intergenic
AAACCAAAGAGAAAGATAAA+GGG 0.427147 5_2:-24622557 None:intergenic
TGAAAGTGTTGATGATGAAA+TGG 0.443172 5_2:-24623846 None:intergenic
AGCACTACAATTTCTGGGGT+TGG 0.454081 5_2:+24622941 Msa0764500:CDS
ATAAGAGCACTACAATTTCT+GGG 0.455946 5_2:+24622936 Msa0764500:CDS
ATTACAGATTATTGCATGCC+AGG 0.468259 5_2:+24623124 Msa0764500:CDS
TACATACCCGTTGTTTGGAC+CGG 0.469355 5_2:+24624064 Msa0764500:three_prime_UTR
TATTGCATGCCAGGAATGAC+TGG 0.472610 5_2:+24623133 Msa0764500:CDS
TCAGAAGACATGATCACTAC+TGG 0.477520 5_2:-24623272 None:intergenic
GGATAGGGATCAACAACAAA+AGG 0.483740 5_2:+24623783 Msa0764500:CDS
ACATCTTGCCACGCATTGAC+AGG 0.484895 5_2:+24623297 Msa0764500:CDS
TAACGATAACTTCAGTGTCT+TGG 0.490936 5_2:-24623724 None:intergenic
GTTGGTAACGGTGTAGGGAT+AGG 0.493017 5_2:+24623767 Msa0764500:CDS
TTCTTAATGATCTTATCCAT+GGG 0.496436 5_2:+24624106 Msa0764500:three_prime_UTR
TCCTTAGCCTGGTTTGAAGG+TGG 0.500088 5_2:+24623093 Msa0764500:intron
GCTTCCCGCTCTAACCTCCC+TGG 0.501953 5_2:-24623702 None:intergenic
ATTGGAGAATCAAGAATGTT+GGG 0.503176 5_2:-24623914 None:intergenic
TACTGGAATTGCTCTAAAAG+TGG 0.506655 5_2:-24623255 None:intergenic
GACAACCAGGGAGGTTAGAG+CGG 0.506833 5_2:+24623697 Msa0764500:CDS
GATTGGAGAATCAAGAATGT+TGG 0.511674 5_2:-24623915 None:intergenic
TTGGTAACGGTGTAGGGATA+GGG 0.512504 5_2:+24623768 Msa0764500:CDS
TTTACATCAGATAATTTCAC+TGG 0.513966 5_2:-24623656 None:intergenic
TAAAGGGGATTCTTTAACGT+AGG 0.531207 5_2:-24622541 None:intergenic
TTGATCGTAAAGTAATTGAA+AGG 0.534403 5_2:+24622689 Msa0764500:CDS
TGTCACAACCTTAAATATTG+AGG 0.535817 5_2:-24622518 None:intergenic
TGACAATCCAAAGAGTCATT+CGG 0.543587 5_2:-24623960 None:intergenic
ATCCGACGGCTTAAAATGAC+TGG 0.550169 5_2:+24623935 Msa0764500:CDS
TACACCGTTACCAACGGTGC+TGG 0.561396 5_2:-24623759 None:intergenic
GCACCGTTGGTAACGGTGTA+GGG 0.562150 5_2:+24623762 Msa0764500:CDS
TCTGATGTAAAACGTTTGAA+GGG 0.564044 5_2:+24623668 Msa0764500:CDS
AACGGTGCTGGTTACAGTGT+TGG 0.567334 5_2:-24623747 None:intergenic
TATTGATGTTGATTCTCAAG+AGG 0.575024 5_2:+24622636 Msa0764500:CDS
TTCTTGATTCTCCAATCCGA+CGG 0.580526 5_2:+24623921 Msa0764500:CDS
GAAGGTGACGGTGATGATGA+TGG 0.581069 5_2:-24623872 None:intergenic
AAACAAAATAAATTAACCCA+TGG 0.592303 5_2:-24624122 None:intergenic
ACTAGATCCACCTTCAAACC+AGG 0.592739 5_2:-24623100 None:intergenic
ACAACCAGGGAGGTTAGAGC+GGG 0.595105 5_2:+24623698 Msa0764500:CDS
AGCACCGTTGGTAACGGTGT+AGG 0.596200 5_2:+24623761 Msa0764500:CDS
TGAAGGGTTACATGACAACC+AGG 0.599838 5_2:+24623684 Msa0764500:CDS
GTAACCAGCACCGTTGGTAA+CGG 0.612875 5_2:+24623755 Msa0764500:CDS
AAATGTAGATGCTTGGAGGA+AGG 0.613553 5_2:+24623611 Msa0764500:intron
AACCAAAGAGAAAGATAAAG+GGG 0.615410 5_2:-24622556 None:intergenic
TTAGTTACTGCAGTGGACAG+TGG 0.615929 5_2:+24622912 Msa0764500:intron
GGATAAGATCATTAAGAACA+AGG 0.618730 5_2:-24624101 None:intergenic
AACACTGTAACCAGCACCGT+TGG 0.627935 5_2:+24623749 Msa0764500:CDS
GAAGACAACGGTGATGATGA+AGG 0.631209 5_2:-24623890 None:intergenic
ATGCTTGGAGGAAGGTGCAG+AGG 0.640084 5_2:+24623619 Msa0764500:CDS
TAAGAGCACTACAATTTCTG+GGG 0.651134 5_2:+24622937 Msa0764500:CDS
TATCCCTACACCGTTACCAA+CGG 0.659748 5_2:-24623765 None:intergenic
GTTGATGATGAAATGGACAG+TGG 0.726606 5_2:-24623839 None:intergenic
AACGGTGATGATGAAGGTGA+CGG 0.733254 5_2:-24623884 None:intergenic
GAAGGGTTACATGACAACCA+GGG 0.738936 5_2:+24623685 Msa0764500:CDS
AGAATGTTGGGTGAAGACAA+CGG 0.739291 5_2:-24623902 None:intergenic
GGGTTACATGACAACCAGGG+AGG 0.751767 5_2:+24623688 Msa0764500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTTGTATTAAAAAATGT+AGG - chr5_2:24623470-24623489 None:intergenic 15.0%
!! TCATATACTTTGAATTAAAT+TGG - chr5_2:24623503-24623522 None:intergenic 15.0%
!! GAAATTATTATGTAGAAAAA+TGG + chr5_2:24623567-24623586 Msa0764500:intron 15.0%
!! TACTTTGTATTAAAAAATGT+AGG - chr5_2:24623470-24623489 None:intergenic 15.0%
!! TCATATACTTTGAATTAAAT+TGG - chr5_2:24623503-24623522 None:intergenic 15.0%
!! GAAATTATTATGTAGAAAAA+TGG + chr5_2:24623567-24623586 Msa0764500:intron 15.0%
!! ACAAAACAAAAAGAAGAAAA+TGG + chr5_2:24622747-24622766 Msa0764500:intron 20.0%
!!! TTTCTTTTGCTCATTTTTAA+AGG + chr5_2:24622772-24622791 Msa0764500:intron 20.0%
!! CAATAAAGTGTAAAAATGAA+GGG - chr5_2:24623173-24623192 None:intergenic 20.0%
!! TCAATAAAGTGTAAAAATGA+AGG - chr5_2:24623174-24623193 None:intergenic 20.0%
!! AAACAAAATAAATTAACCCA+TGG - chr5_2:24624125-24624144 None:intergenic 20.0%
!!! TTTGTTTTTTAGTATGAAAG+TGG + chr5_2:24624138-24624157 Msa0764500:three_prime_UTR 20.0%
!!! TTGTTTTTTAGTATGAAAGT+GGG + chr5_2:24624139-24624158 Msa0764500:three_prime_UTR 20.0%
!! GTAAAAAAAGAAGTAGAAAT+TGG + chr5_2:24624166-24624185 Msa0764500:three_prime_UTR 20.0%
!! ATCCAAAAAATTATGAAAGT+GGG + chr5_2:24624189-24624208 Msa0764500:three_prime_UTR 20.0%
!! TTCATCTTTAGTAAAGAAAA+AGG - chr5_2:24624252-24624271 None:intergenic 20.0%
!! ACAAAACAAAAAGAAGAAAA+TGG + chr5_2:24622747-24622766 Msa0764500:intron 20.0%
!!! TTTCTTTTGCTCATTTTTAA+AGG + chr5_2:24622772-24622791 Msa0764500:intron 20.0%
!! CAATAAAGTGTAAAAATGAA+GGG - chr5_2:24623173-24623192 None:intergenic 20.0%
!! TCAATAAAGTGTAAAAATGA+AGG - chr5_2:24623174-24623193 None:intergenic 20.0%
!! AAACAAAATAAATTAACCCA+TGG - chr5_2:24624125-24624144 None:intergenic 20.0%
!!! TTTGTTTTTTAGTATGAAAG+TGG + chr5_2:24624138-24624157 Msa0764500:three_prime_UTR 20.0%
!!! TTGTTTTTTAGTATGAAAGT+GGG + chr5_2:24624139-24624158 Msa0764500:three_prime_UTR 20.0%
!! GTAAAAAAAGAAGTAGAAAT+TGG + chr5_2:24624166-24624185 Msa0764500:three_prime_UTR 20.0%
!! ATCCAAAAAATTATGAAAGT+GGG + chr5_2:24624189-24624208 Msa0764500:three_prime_UTR 20.0%
!! TTCATCTTTAGTAAAGAAAA+AGG - chr5_2:24624252-24624271 None:intergenic 20.0%
! ATATCACTCCTCAATATTTA+AGG + chr5_2:24622510-24622529 Msa0764500:five_prime_UTR 25.0%
! AAACCAAAGAGAAAGATAAA+GGG - chr5_2:24622560-24622579 None:intergenic 25.0%
!! CTCTTTGAAGAAAAAAAAGA+GGG - chr5_2:24622589-24622608 None:intergenic 25.0%
!! TCTCTTTGAAGAAAAAAAAG+AGG - chr5_2:24622590-24622609 None:intergenic 25.0%
! TTGATCGTAAAGTAATTGAA+AGG + chr5_2:24622689-24622708 Msa0764500:CDS 25.0%
! CTCAAAATTTCTTCTTGTAA+AGG + chr5_2:24622715-24622734 Msa0764500:CDS 25.0%
!!! TTTATTTTTAGTTACTGCAG+TGG + chr5_2:24622905-24622924 Msa0764500:intron 25.0%
! ATTGAAAAAATGTAGATGCT+TGG + chr5_2:24623604-24623623 Msa0764500:intron 25.0%
! TTTACATCAGATAATTTCAC+TGG - chr5_2:24623659-24623678 None:intergenic 25.0%
!! TGCATTTTAAACATCAAAAG+AGG - chr5_2:24624042-24624061 None:intergenic 25.0%
! TTCTTAATGATCTTATCCAT+GGG + chr5_2:24624106-24624125 Msa0764500:three_prime_UTR 25.0%
! GATCCAAAAAATTATGAAAG+TGG + chr5_2:24624188-24624207 Msa0764500:three_prime_UTR 25.0%
!!! AACCCACTTTCATAATTTTT+TGG - chr5_2:24624194-24624213 None:intergenic 25.0%
! ATATCACTCCTCAATATTTA+AGG + chr5_2:24622510-24622529 Msa0764500:five_prime_UTR 25.0%
! AAACCAAAGAGAAAGATAAA+GGG - chr5_2:24622560-24622579 None:intergenic 25.0%
!! CTCTTTGAAGAAAAAAAAGA+GGG - chr5_2:24622589-24622608 None:intergenic 25.0%
!! TCTCTTTGAAGAAAAAAAAG+AGG - chr5_2:24622590-24622609 None:intergenic 25.0%
! TTGATCGTAAAGTAATTGAA+AGG + chr5_2:24622689-24622708 Msa0764500:CDS 25.0%
! CTCAAAATTTCTTCTTGTAA+AGG + chr5_2:24622715-24622734 Msa0764500:CDS 25.0%
!!! TTTATTTTTAGTTACTGCAG+TGG + chr5_2:24622905-24622924 Msa0764500:intron 25.0%
! ATTGAAAAAATGTAGATGCT+TGG + chr5_2:24623604-24623623 Msa0764500:intron 25.0%
! TTTACATCAGATAATTTCAC+TGG - chr5_2:24623659-24623678 None:intergenic 25.0%
!! TGCATTTTAAACATCAAAAG+AGG - chr5_2:24624042-24624061 None:intergenic 25.0%
! TTCTTAATGATCTTATCCAT+GGG + chr5_2:24624106-24624125 Msa0764500:three_prime_UTR 25.0%
! GATCCAAAAAATTATGAAAG+TGG + chr5_2:24624188-24624207 Msa0764500:three_prime_UTR 25.0%
!!! AACCCACTTTCATAATTTTT+TGG - chr5_2:24624194-24624213 None:intergenic 25.0%
TGTCACAACCTTAAATATTG+AGG - chr5_2:24622521-24622540 None:intergenic 30.0%
AACCAAAGAGAAAGATAAAG+GGG - chr5_2:24622559-24622578 None:intergenic 30.0%
GAAACCAAAGAGAAAGATAA+AGG - chr5_2:24622561-24622580 None:intergenic 30.0%
! TTTTTCTTCAAAGAGAGTGA+TGG + chr5_2:24622594-24622613 Msa0764500:exon 30.0%
!! TATTGATGTTGATTCTCAAG+AGG + chr5_2:24622636-24622655 Msa0764500:CDS 30.0%
!! AATAAGAGCACTACAATTTC+TGG + chr5_2:24622935-24622954 Msa0764500:CDS 30.0%
!! ATAAGAGCACTACAATTTCT+GGG + chr5_2:24622936-24622955 Msa0764500:CDS 30.0%
!!! TTTATTTTGTTTCCTTAGCC+TGG + chr5_2:24623082-24623101 Msa0764500:intron 30.0%
ATCTGATGTAAAACGTTTGA+AGG + chr5_2:24623667-24623686 Msa0764500:CDS 30.0%
TCTGATGTAAAACGTTTGAA+GGG + chr5_2:24623668-24623687 Msa0764500:CDS 30.0%
!! TGAAAGTGTTGATGATGAAA+TGG - chr5_2:24623849-24623868 None:intergenic 30.0%
ATTGGAGAATCAAGAATGTT+GGG - chr5_2:24623917-24623936 None:intergenic 30.0%
GGATAAGATCATTAAGAACA+AGG - chr5_2:24624104-24624123 None:intergenic 30.0%
GTTCTTAATGATCTTATCCA+TGG + chr5_2:24624105-24624124 Msa0764500:three_prime_UTR 30.0%
TGTCACAACCTTAAATATTG+AGG - chr5_2:24622521-24622540 None:intergenic 30.0%
AACCAAAGAGAAAGATAAAG+GGG - chr5_2:24622559-24622578 None:intergenic 30.0%
GAAACCAAAGAGAAAGATAA+AGG - chr5_2:24622561-24622580 None:intergenic 30.0%
! TTTTTCTTCAAAGAGAGTGA+TGG + chr5_2:24622594-24622613 Msa0764500:exon 30.0%
!! TATTGATGTTGATTCTCAAG+AGG + chr5_2:24622636-24622655 Msa0764500:CDS 30.0%
!! AATAAGAGCACTACAATTTC+TGG + chr5_2:24622935-24622954 Msa0764500:CDS 30.0%
!! ATAAGAGCACTACAATTTCT+GGG + chr5_2:24622936-24622955 Msa0764500:CDS 30.0%
!!! TTTATTTTGTTTCCTTAGCC+TGG + chr5_2:24623082-24623101 Msa0764500:intron 30.0%
ATCTGATGTAAAACGTTTGA+AGG + chr5_2:24623667-24623686 Msa0764500:CDS 30.0%
TCTGATGTAAAACGTTTGAA+GGG + chr5_2:24623668-24623687 Msa0764500:CDS 30.0%
!! TGAAAGTGTTGATGATGAAA+TGG - chr5_2:24623849-24623868 None:intergenic 30.0%
ATTGGAGAATCAAGAATGTT+GGG - chr5_2:24623917-24623936 None:intergenic 30.0%
GGATAAGATCATTAAGAACA+AGG - chr5_2:24624104-24624123 None:intergenic 30.0%
GTTCTTAATGATCTTATCCA+TGG + chr5_2:24624105-24624124 Msa0764500:three_prime_UTR 30.0%
TAAAGGGGATTCTTTAACGT+AGG - chr5_2:24622544-24622563 None:intergenic 35.0%
ATCCCCTTTATCTTTCTCTT+TGG + chr5_2:24622554-24622573 Msa0764500:five_prime_UTR 35.0%
! TTCTCAAGAGGTTCATGTTT+TGG + chr5_2:24622648-24622667 Msa0764500:CDS 35.0%
!! TAAGAGCACTACAATTTCTG+GGG + chr5_2:24622937-24622956 Msa0764500:CDS 35.0%
ATTACAGATTATTGCATGCC+AGG + chr5_2:24623124-24623143 Msa0764500:CDS 35.0%
! TCCAAGTTTCAATTTGAAGC+AGG + chr5_2:24623226-24623245 Msa0764500:intron 35.0%
TCCTGCTTCAAATTGAAACT+TGG - chr5_2:24623230-24623249 None:intergenic 35.0%
!! TACTGGAATTGCTCTAAAAG+TGG - chr5_2:24623258-24623277 None:intergenic 35.0%
AAAAAATACCTGTCAATGCG+TGG - chr5_2:24623308-24623327 None:intergenic 35.0%
GAAAAAATGTAGATGCTTGG+AGG + chr5_2:24623607-24623626 Msa0764500:intron 35.0%
TAACGATAACTTCAGTGTCT+TGG - chr5_2:24623727-24623746 None:intergenic 35.0%
GATTGGAGAATCAAGAATGT+TGG - chr5_2:24623918-24623937 None:intergenic 35.0%
TGACAATCCAAAGAGTCATT+CGG - chr5_2:24623963-24623982 None:intergenic 35.0%
GAGGTGAACAAGTAAATGTT+TGG - chr5_2:24624023-24624042 None:intergenic 35.0%
TAAAGGGGATTCTTTAACGT+AGG - chr5_2:24622544-24622563 None:intergenic 35.0%
ATCCCCTTTATCTTTCTCTT+TGG + chr5_2:24622554-24622573 Msa0764500:five_prime_UTR 35.0%
! TTCTCAAGAGGTTCATGTTT+TGG + chr5_2:24622648-24622667 Msa0764500:CDS 35.0%
!! TAAGAGCACTACAATTTCTG+GGG + chr5_2:24622937-24622956 Msa0764500:CDS 35.0%
ATTACAGATTATTGCATGCC+AGG + chr5_2:24623124-24623143 Msa0764500:CDS 35.0%
! TCCAAGTTTCAATTTGAAGC+AGG + chr5_2:24623226-24623245 Msa0764500:intron 35.0%
TCCTGCTTCAAATTGAAACT+TGG - chr5_2:24623230-24623249 None:intergenic 35.0%
!! TACTGGAATTGCTCTAAAAG+TGG - chr5_2:24623258-24623277 None:intergenic 35.0%
AAAAAATACCTGTCAATGCG+TGG - chr5_2:24623308-24623327 None:intergenic 35.0%
GAAAAAATGTAGATGCTTGG+AGG + chr5_2:24623607-24623626 Msa0764500:intron 35.0%
TAACGATAACTTCAGTGTCT+TGG - chr5_2:24623727-24623746 None:intergenic 35.0%
GATTGGAGAATCAAGAATGT+TGG - chr5_2:24623918-24623937 None:intergenic 35.0%
TGACAATCCAAAGAGTCATT+CGG - chr5_2:24623963-24623982 None:intergenic 35.0%
GAGGTGAACAAGTAAATGTT+TGG - chr5_2:24624023-24624042 None:intergenic 35.0%
TTGTGATAACCAGTCATTCC+TGG - chr5_2:24623145-24623164 None:intergenic 40.0%
TCAGAAGACATGATCACTAC+TGG - chr5_2:24623275-24623294 None:intergenic 40.0%
AAATGTAGATGCTTGGAGGA+AGG + chr5_2:24623611-24623630 Msa0764500:intron 40.0%
GGATAGGGATCAACAACAAA+AGG + chr5_2:24623783-24623802 Msa0764500:CDS 40.0%
!! GTTGATGATGAAATGGACAG+TGG - chr5_2:24623842-24623861 None:intergenic 40.0%
AGAATGTTGGGTGAAGACAA+CGG - chr5_2:24623905-24623924 None:intergenic 40.0%
TTCTTGATTCTCCAATCCGA+CGG + chr5_2:24623921-24623940 Msa0764500:CDS 40.0%
GCAATTACATACCCGTTGTT+TGG + chr5_2:24624059-24624078 Msa0764500:three_prime_UTR 40.0%
ACAAAAACCGGTCCAAACAA+CGG - chr5_2:24624074-24624093 None:intergenic 40.0%
CAAGGCAATGCAACAAAAAC+CGG - chr5_2:24624086-24624105 None:intergenic 40.0%
TTGTGATAACCAGTCATTCC+TGG - chr5_2:24623145-24623164 None:intergenic 40.0%
TCAGAAGACATGATCACTAC+TGG - chr5_2:24623275-24623294 None:intergenic 40.0%
AAATGTAGATGCTTGGAGGA+AGG + chr5_2:24623611-24623630 Msa0764500:intron 40.0%
GGATAGGGATCAACAACAAA+AGG + chr5_2:24623783-24623802 Msa0764500:CDS 40.0%
!! GTTGATGATGAAATGGACAG+TGG - chr5_2:24623842-24623861 None:intergenic 40.0%
AGAATGTTGGGTGAAGACAA+CGG - chr5_2:24623905-24623924 None:intergenic 40.0%
TTCTTGATTCTCCAATCCGA+CGG + chr5_2:24623921-24623940 Msa0764500:CDS 40.0%
GCAATTACATACCCGTTGTT+TGG + chr5_2:24624059-24624078 Msa0764500:three_prime_UTR 40.0%
ACAAAAACCGGTCCAAACAA+CGG - chr5_2:24624074-24624093 None:intergenic 40.0%
CAAGGCAATGCAACAAAAAC+CGG - chr5_2:24624086-24624105 None:intergenic 40.0%
TTAGTTACTGCAGTGGACAG+TGG + chr5_2:24622912-24622931 Msa0764500:intron 45.0%
!! AGCACTACAATTTCTGGGGT+TGG + chr5_2:24622941-24622960 Msa0764500:CDS 45.0%
! GTTTCCTTAGCCTGGTTTGA+AGG + chr5_2:24623090-24623109 Msa0764500:intron 45.0%
ACTAGATCCACCTTCAAACC+AGG - chr5_2:24623103-24623122 None:intergenic 45.0%
TATTGCATGCCAGGAATGAC+TGG + chr5_2:24623133-24623152 Msa0764500:CDS 45.0%
TGAAGGGTTACATGACAACC+AGG + chr5_2:24623684-24623703 Msa0764500:CDS 45.0%
GAAGGGTTACATGACAACCA+GGG + chr5_2:24623685-24623704 Msa0764500:CDS 45.0%
TATCCCTACACCGTTACCAA+CGG - chr5_2:24623768-24623787 None:intergenic 45.0%
!! TTGGTAACGGTGTAGGGATA+GGG + chr5_2:24623768-24623787 Msa0764500:CDS 45.0%
! AACGGTGATGATGAAGGTGA+CGG - chr5_2:24623887-24623906 None:intergenic 45.0%
! GAAGACAACGGTGATGATGA+AGG - chr5_2:24623893-24623912 None:intergenic 45.0%
!! GTCATTTTAAGCCGTCGGAT+TGG - chr5_2:24623935-24623954 None:intergenic 45.0%
ATCCGACGGCTTAAAATGAC+TGG + chr5_2:24623935-24623954 Msa0764500:CDS 45.0%
!! TGCCAGTCATTTTAAGCCGT+CGG - chr5_2:24623940-24623959 None:intergenic 45.0%
TACATACCCGTTGTTTGGAC+CGG + chr5_2:24624064-24624083 Msa0764500:three_prime_UTR 45.0%
CAAAAACCGGTCCAAACAAC+GGG - chr5_2:24624073-24624092 None:intergenic 45.0%
TTAGTTACTGCAGTGGACAG+TGG + chr5_2:24622912-24622931 Msa0764500:intron 45.0%
!! AGCACTACAATTTCTGGGGT+TGG + chr5_2:24622941-24622960 Msa0764500:CDS 45.0%
! GTTTCCTTAGCCTGGTTTGA+AGG + chr5_2:24623090-24623109 Msa0764500:intron 45.0%
ACTAGATCCACCTTCAAACC+AGG - chr5_2:24623103-24623122 None:intergenic 45.0%
TATTGCATGCCAGGAATGAC+TGG + chr5_2:24623133-24623152 Msa0764500:CDS 45.0%
TGAAGGGTTACATGACAACC+AGG + chr5_2:24623684-24623703 Msa0764500:CDS 45.0%
GAAGGGTTACATGACAACCA+GGG + chr5_2:24623685-24623704 Msa0764500:CDS 45.0%
TATCCCTACACCGTTACCAA+CGG - chr5_2:24623768-24623787 None:intergenic 45.0%
!! TTGGTAACGGTGTAGGGATA+GGG + chr5_2:24623768-24623787 Msa0764500:CDS 45.0%
! AACGGTGATGATGAAGGTGA+CGG - chr5_2:24623887-24623906 None:intergenic 45.0%
! GAAGACAACGGTGATGATGA+AGG - chr5_2:24623893-24623912 None:intergenic 45.0%
!! GTCATTTTAAGCCGTCGGAT+TGG - chr5_2:24623935-24623954 None:intergenic 45.0%
ATCCGACGGCTTAAAATGAC+TGG + chr5_2:24623935-24623954 Msa0764500:CDS 45.0%
!! TGCCAGTCATTTTAAGCCGT+CGG - chr5_2:24623940-24623959 None:intergenic 45.0%
TACATACCCGTTGTTTGGAC+CGG + chr5_2:24624064-24624083 Msa0764500:three_prime_UTR 45.0%
CAAAAACCGGTCCAAACAAC+GGG - chr5_2:24624073-24624092 None:intergenic 45.0%
!! CTAAAATAAAAAATTTAAAT+TGG - chr5_2:24623012-24623031 None:intergenic 5.0%
!!! ATTTAAATTTTTTATTTTAG+CGG + chr5_2:24623012-24623031 Msa0764500:intron 5.0%
!! CTAAAATAAAAAATTTAAAT+TGG - chr5_2:24623012-24623031 None:intergenic 5.0%
!!! ATTTAAATTTTTTATTTTAG+CGG + chr5_2:24623012-24623031 Msa0764500:intron 5.0%
! TCCTTAGCCTGGTTTGAAGG+TGG + chr5_2:24623093-24623112 Msa0764500:intron 50.0%
TCCACCTTCAAACCAGGCTA+AGG - chr5_2:24623097-24623116 None:intergenic 50.0%
! ACATCTTGCCACGCATTGAC+AGG + chr5_2:24623297-24623316 Msa0764500:CDS 50.0%
!! AACGGTGCTGGTTACAGTGT+TGG - chr5_2:24623750-24623769 None:intergenic 50.0%
AACACTGTAACCAGCACCGT+TGG + chr5_2:24623749-24623768 Msa0764500:CDS 50.0%
! GTAACCAGCACCGTTGGTAA+CGG + chr5_2:24623755-24623774 Msa0764500:CDS 50.0%
!! GTTGGTAACGGTGTAGGGAT+AGG + chr5_2:24623767-24623786 Msa0764500:CDS 50.0%
! GAAGGTGACGGTGATGATGA+TGG - chr5_2:24623875-24623894 None:intergenic 50.0%
! ACTGGCACCGAATGACTCTT+TGG + chr5_2:24623953-24623972 Msa0764500:exon 50.0%
! TCCTTAGCCTGGTTTGAAGG+TGG + chr5_2:24623093-24623112 Msa0764500:intron 50.0%
TCCACCTTCAAACCAGGCTA+AGG - chr5_2:24623097-24623116 None:intergenic 50.0%
! ACATCTTGCCACGCATTGAC+AGG + chr5_2:24623297-24623316 Msa0764500:CDS 50.0%
!! AACGGTGCTGGTTACAGTGT+TGG - chr5_2:24623750-24623769 None:intergenic 50.0%
AACACTGTAACCAGCACCGT+TGG + chr5_2:24623749-24623768 Msa0764500:CDS 50.0%
! GTAACCAGCACCGTTGGTAA+CGG + chr5_2:24623755-24623774 Msa0764500:CDS 50.0%
!! GTTGGTAACGGTGTAGGGAT+AGG + chr5_2:24623767-24623786 Msa0764500:CDS 50.0%
! GAAGGTGACGGTGATGATGA+TGG - chr5_2:24623875-24623894 None:intergenic 50.0%
! ACTGGCACCGAATGACTCTT+TGG + chr5_2:24623953-24623972 Msa0764500:exon 50.0%
! ATGCTTGGAGGAAGGTGCAG+AGG + chr5_2:24623619-24623638 Msa0764500:CDS 55.0%
GGGTTACATGACAACCAGGG+AGG + chr5_2:24623688-24623707 Msa0764500:CDS 55.0%
GACAACCAGGGAGGTTAGAG+CGG + chr5_2:24623697-24623716 Msa0764500:CDS 55.0%
ACAACCAGGGAGGTTAGAGC+GGG + chr5_2:24623698-24623717 Msa0764500:CDS 55.0%
TACACCGTTACCAACGGTGC+TGG - chr5_2:24623762-24623781 None:intergenic 55.0%
!! AGCACCGTTGGTAACGGTGT+AGG + chr5_2:24623761-24623780 Msa0764500:CDS 55.0%
!! GCACCGTTGGTAACGGTGTA+GGG + chr5_2:24623762-24623781 Msa0764500:CDS 55.0%
! ATGCTTGGAGGAAGGTGCAG+AGG + chr5_2:24623619-24623638 Msa0764500:CDS 55.0%
GGGTTACATGACAACCAGGG+AGG + chr5_2:24623688-24623707 Msa0764500:CDS 55.0%
GACAACCAGGGAGGTTAGAG+CGG + chr5_2:24623697-24623716 Msa0764500:CDS 55.0%
ACAACCAGGGAGGTTAGAGC+GGG + chr5_2:24623698-24623717 Msa0764500:CDS 55.0%
TACACCGTTACCAACGGTGC+TGG - chr5_2:24623762-24623781 None:intergenic 55.0%
!! AGCACCGTTGGTAACGGTGT+AGG + chr5_2:24623761-24623780 Msa0764500:CDS 55.0%
!! GCACCGTTGGTAACGGTGTA+GGG + chr5_2:24623762-24623781 Msa0764500:CDS 55.0%
GCTTCCCGCTCTAACCTCCC+TGG - chr5_2:24623705-24623724 None:intergenic 65.0%
GCTTCCCGCTCTAACCTCCC+TGG - chr5_2:24623705-24623724 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 24622498 24624296 24622498 ID=Msa0764500;Name=Msa0764500
chr5_2 mRNA 24622498 24624296 24622498 ID=Msa0764500-mRNA-1;Parent=Msa0764500;Name=Msa0764500-mRNA-1;_AED=0.08;_eAED=0.08;_QI=115|0.75|0.6|1|1|1|5|329|244
chr5_2 exon 24622498 24622736 24622498 ID=Msa0764500-mRNA-1:exon:9984;Parent=Msa0764500-mRNA-1
chr5_2 exon 24622916 24623001 24622916 ID=Msa0764500-mRNA-1:exon:9985;Parent=Msa0764500-mRNA-1
chr5_2 exon 24623100 24623204 24623100 ID=Msa0764500-mRNA-1:exon:9986;Parent=Msa0764500-mRNA-1
chr5_2 exon 24623248 24623318 24623248 ID=Msa0764500-mRNA-1:exon:9987;Parent=Msa0764500-mRNA-1
chr5_2 exon 24623619 24624296 24623619 ID=Msa0764500-mRNA-1:exon:9988;Parent=Msa0764500-mRNA-1
chr5_2 five_prime_UTR 24622498 24622612 24622498 ID=Msa0764500-mRNA-1:five_prime_utr;Parent=Msa0764500-mRNA-1
chr5_2 CDS 24622613 24622736 24622613 ID=Msa0764500-mRNA-1:cds;Parent=Msa0764500-mRNA-1
chr5_2 CDS 24622916 24623001 24622916 ID=Msa0764500-mRNA-1:cds;Parent=Msa0764500-mRNA-1
chr5_2 CDS 24623100 24623204 24623100 ID=Msa0764500-mRNA-1:cds;Parent=Msa0764500-mRNA-1
chr5_2 CDS 24623248 24623318 24623248 ID=Msa0764500-mRNA-1:cds;Parent=Msa0764500-mRNA-1
chr5_2 CDS 24623619 24623967 24623619 ID=Msa0764500-mRNA-1:cds;Parent=Msa0764500-mRNA-1
chr5_2 three_prime_UTR 24623968 24624296 24623968 ID=Msa0764500-mRNA-1:three_prime_utr;Parent=Msa0764500-mRNA-1
Gene Sequence

>Msa0764500

ATGGAGACAAACAGTGTTGTTTCTATTGATGTTGATTCTCAAGAGGTTCATGTTTTGGCTGTTGATGATAGTCTTGTTGATCGTAAAGTAATTGAAAGGTTGCTCAAAATTTCTTCTTGTAAAGTTACTGCAGTGGACAGTGGAATAAGAGCACTACAATTTCTGGGGTTGGTTGATGAGAAAGAGACTTCTGAATCTGATGCTTTTGTTCCTGGTTTGAAGGTGGATCTAGTAATTACAGATTATTGCATGCCAGGAATGACTGGTTATCACAAACAATCCCTTCATTTTTACACTTTATTGATTGATTATAATGAATCATCCACTTTTAGAGCAATTCCAGTAGTGATCATGTCTTCTGAAAACATCTTGCCACGCATTGACAGATGCTTGGAGGAAGGTGCAGAGGATTTCATAGTGAAGCCAGTGAAATTATCTGATGTAAAACGTTTGAAGGGTTACATGACAACCAGGGAGGTTAGAGCGGGAAGCCAAGACACTGAAGTTATCGTTACCAACACTGTAACCAGCACCGTTGGTAACGGTGTAGGGATAGGGATCAACAACAAAAGGAAGCTTGAAGAAGCATCTGATTTGTCATCATCACCACTGTCCATTTCATCATCAACACTTTCATCACCATCATCATCACCGTCACCTTCATCATCACCGTTGTCTTCACCCAACATTCTTGATTCTCCAATCCGACGGCTTAAAATGACTGGCACCGAATGA
Protein sequence

>Msa0764500

METNSVVSIDVDSQEVHVLAVDDSLVDRKVIERLLKISSCKVTAVDSGIRALQFLGLVDEKETSESDAFVPGLKVDLVITDYCMPGMTGYHKQSLHFYTLLIDYNESSTFRAIPVVIMSSENILPRIDRCLEEGAEDFIVKPVKLSDVKRLKGYMTTREVRAGSQDTEVIVTNTVTSTVGNGVGIGINNKRKLEEASDLSSSPLSISSSTLSSPSSSPSPSSSPLSSPNILDSPIRRLKMTGTE*