AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0764660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0764660 MtrunA17_Chr7g0243051 86.000 50 7 0 92 141 153 202 5.96e-22 88.6
Msa0764660 MtrunA17_Chr4g0007601 57.143 56 24 0 92 147 137 192 3.01e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0764660 AT3G51780.1 44.554 101 46 2 41 141 115 205 1.04e-15 73.2

Find 94 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAATTGTATTGATTTAT+TGG 0.104172 5_2:-24960925 Msa0764660:three_prime_UTR
AATCTCTATGATGTCATTTA+AGG 0.161713 5_2:-24960990 Msa0764660:three_prime_UTR
AAAAGTGCGTTATATAATTT+TGG 0.163153 5_2:-24960068 Msa0764660:three_prime_UTR
TTTGGTGTTCAAGTTGAAAT+AGG 0.189818 5_2:-24960111 Msa0764660:three_prime_UTR
GCATTTGATTGCTCTGTTTC+GGG 0.251674 5_2:-24961262 Msa0764660:CDS
CGTTGATTTGATTGAAGTTT+TGG 0.252157 5_2:-24960161 Msa0764660:three_prime_UTR
CTTAGACATAGTAAGCTTAC+AGG 0.258182 5_2:+24959992 None:intergenic
CAATTACATGGTAAGCTTAC+AGG 0.259734 5_2:+24959966 None:intergenic
TTTCTGTTTCGATGGTGATT+AGG 0.264345 5_2:-24962559 Msa0764660:CDS
GGCATTTCAAGTTGCAATAA+TGG 0.264466 5_2:-24962388 Msa0764660:CDS
TTCTGTTTCGATGGTGATTA+GGG 0.268495 5_2:-24962558 Msa0764660:CDS
TGCATTTGATTGCTCTGTTT+CGG 0.276018 5_2:-24961263 Msa0764660:CDS
TCAATTGCTCGACCGATTCC+TGG 0.321499 5_2:-24960896 Msa0764660:three_prime_UTR
TATGCATGTAAAGACAATAA+TGG 0.325264 5_2:+24960446 None:intergenic
AAAGCACATGCTCTTCTATC+TGG 0.336971 5_2:-24960239 Msa0764660:three_prime_UTR
TATTCCTTGGCTCATCTAAT+AGG 0.338115 5_2:+24960390 None:intergenic
TTGGTGTTCAAGTTGAAATA+GGG 0.340448 5_2:-24960110 Msa0764660:three_prime_UTR
TTATTGTCTTTACATGCATA+TGG 0.358776 5_2:-24960443 Msa0764660:three_prime_UTR
AAGAGACTGGTCAAGCTATT+AGG 0.365063 5_2:-24962323 Msa0764660:CDS
GACCAAAGTGCTAGATATAA+TGG 0.367410 5_2:-24960734 Msa0764660:three_prime_UTR
GCTGAAGGTGGATGGTATTA+AGG 0.373063 5_2:-24961758 Msa0764660:CDS
GCGTTGTTGGTTGATGTTGT+AGG 0.374231 5_2:+24961043 None:intergenic
AAGTAGAATCATGGACAATT+TGG 0.378644 5_2:-24960129 Msa0764660:three_prime_UTR
ATGCATGTAAAGACAATAAT+GGG 0.379762 5_2:+24960447 None:intergenic
TTGGCTCAATCTCGCGTTGT+TGG 0.389258 5_2:+24961030 None:intergenic
CAAGTTGCAATAATGGATAC+AGG 0.389553 5_2:-24962381 Msa0764660:CDS
CTCTCTGAATCGCGTCCAAC+TGG 0.394223 5_2:-24962498 Msa0764660:intron
TAATGCAGATTGGCCTCAAC+AGG 0.398732 5_2:-24962522 Msa0764660:CDS
CTGTTTCGGGTTGACTTCGC+AGG 0.406464 5_2:-24961249 Msa0764660:CDS
GTCTTGGATGTGGCTGTCAA+TGG 0.409273 5_2:-24961841 Msa0764660:CDS
CATGTGCTTTAAGAAATTAT+AGG 0.414034 5_2:+24960252 None:intergenic
CGGGTTGACTTCGCAGGAAG+AGG 0.421872 5_2:-24961243 Msa0764660:CDS
AAAAGGGTTTCGAAGTTTCC+AGG 0.425046 5_2:-24960696 Msa0764660:three_prime_UTR
AGGAGAGCTTGCTGAGCGCC+AGG 0.429092 5_2:-24961223 Msa0764660:intron
ATGAATCTGCCATTTGCAAT+AGG 0.433789 5_2:+24960471 None:intergenic
TTTACATTGACTCTATTCCT+TGG 0.441317 5_2:+24960377 None:intergenic
AGTTTCCAGGATTGACGGTT+CGG 0.445988 5_2:-24960683 Msa0764660:three_prime_UTR
TTTAGATCAATTGTCTCCCT+TGG 0.447551 5_2:+24961011 None:intergenic
ATTGCAGCCTATGGTTCTAA+AGG 0.449394 5_2:-24962199 Msa0764660:intron
GTTCGGTGAAGCTGGATGAT+AGG 0.450647 5_2:-24960666 Msa0764660:three_prime_UTR
GATTGACGGTTCGGTGAAGC+TGG 0.453321 5_2:-24960674 Msa0764660:three_prime_UTR
CATGTGTTTATAATGCAGAT+TGG 0.453463 5_2:-24962532 Msa0764660:CDS
AAATTCTTTGTCAGAAACCT+TGG 0.460069 5_2:+24961813 None:intergenic
CTTCACCGAACCGTCAATCC+TGG 0.462843 5_2:+24960678 None:intergenic
CAAATAGCCGCTCCAGGAAT+CGG 0.463361 5_2:+24960884 None:intergenic
GCAGGTGTTGGTCTTGGATG+TGG 0.467678 5_2:-24961851 Msa0764660:intron
CATAACACCAAAAGGACAAA+AGG 0.470282 5_2:+24960564 None:intergenic
ATCCATTATATCTAGCACTT+TGG 0.479735 5_2:+24960732 None:intergenic
AGAAAATGCATAACACCAAA+AGG 0.486251 5_2:+24960556 None:intergenic
TTCGAAGTTTCCAGGATTGA+CGG 0.486372 5_2:-24960688 Msa0764660:three_prime_UTR
ATCTAATAGGAACATGCAAA+TGG 0.486424 5_2:+24960403 None:intergenic
ACTTTCTTTCCTATTGCAAA+TGG 0.495002 5_2:-24960480 Msa0764660:three_prime_UTR
CTTAATGCAAAGTAGAATCA+TGG 0.495923 5_2:-24960138 Msa0764660:three_prime_UTR
ATACATGGAATTTGAAGAGC+TGG 0.496783 5_2:-24962171 Msa0764660:CDS
TTCACTACAGGATCTGTTGG+AGG 0.502861 5_2:-24962409 Msa0764660:intron
TGGTGTTCAAGTTGAAATAG+GGG 0.503556 5_2:-24960109 Msa0764660:three_prime_UTR
TTGGATGTGGCTGTCAATGG+AGG 0.505626 5_2:-24961838 Msa0764660:CDS
GGCAGGTCTGTATCAATGCT+TGG 0.505815 5_2:-24960639 Msa0764660:three_prime_UTR
GGTCAAGCTATTAGGTCCAC+TGG 0.511994 5_2:-24962315 Msa0764660:intron
TCCGTGCAAATAGCCGCTCC+AGG 0.524954 5_2:+24960878 None:intergenic
GTCGGCGACGGCGAGATTGA+CGG 0.530471 5_2:+24962912 None:intergenic
TCCTGGAGCGGCTATTTGCA+CGG 0.542718 5_2:-24960879 Msa0764660:three_prime_UTR
TGACTACAGAGTTACTTATG+AGG 0.552070 5_2:-24961786 Msa0764660:CDS
AATTATAAGCATAATTCAGA+TGG 0.556625 5_2:-24960512 Msa0764660:three_prime_UTR
GTTCTGCTTATCAAAGAGAC+TGG 0.560977 5_2:-24962336 Msa0764660:CDS
GTTCTAAAGGAGTTGATACA+TGG 0.564029 5_2:-24962186 Msa0764660:CDS
TACAGTTTGTAATTGATGCA+AGG 0.566888 5_2:-24959896 Msa0764660:three_prime_UTR
AGGTTCTGATCTTCAATCAG+TGG 0.567106 5_2:-24960756 Msa0764660:three_prime_UTR
TGAAGCTGGATGATAGGAAA+CGG 0.574761 5_2:-24960660 Msa0764660:three_prime_UTR
GCTGGATGATAGGAAACGGC+AGG 0.584808 5_2:-24960656 Msa0764660:three_prime_UTR
AGGAAATTGCTGAAGGTGGA+TGG 0.586998 5_2:-24961766 Msa0764660:CDS
GAATTTGAAGAGCTGGCAGA+AGG 0.589098 5_2:-24962164 Msa0764660:CDS
ACAGTGAAGATCGCAGGTGA+CGG 0.593330 5_2:+24962937 None:intergenic
ATCAACTCCTTTAGAACCAT+AGG 0.595242 5_2:+24962192 None:intergenic
ACGGCGACAGTGAAGATCGC+AGG 0.595661 5_2:+24962931 None:intergenic
TTAAGGCTGAAGGTGATGCT+AGG 0.600335 5_2:-24961741 Msa0764660:CDS
AGTGAGAACAACAGTTTGTG+TGG 0.607355 5_2:-24959840 Msa0764660:three_prime_UTR
TGGATGTGGCTGTCAATGGA+GGG 0.610730 5_2:-24961837 Msa0764660:CDS
GTGGATGGTATTAAGGCTGA+AGG 0.610870 5_2:-24961751 Msa0764660:CDS
GGCTGTCAATGGAGGGACCA+AGG 0.612660 5_2:-24961830 Msa0764660:CDS
ACTTATGAGGAAATTGCTGA+AGG 0.615202 5_2:-24961773 Msa0764660:CDS
TGCTCGACCGATTCCTGGAG+CGG 0.617065 5_2:-24960891 Msa0764660:three_prime_UTR
TATGAGGAAATTGCTGAAGG+TGG 0.618935 5_2:-24961770 Msa0764660:CDS
TGTTCCTATTAGATGAGCCA+AGG 0.620048 5_2:-24960394 Msa0764660:three_prime_UTR
ACAACGCGAGATTGAGCCAA+GGG 0.632853 5_2:-24961027 Msa0764660:CDS
TTGACTAAAAGCACATCACA+AGG 0.643518 5_2:-24959946 Msa0764660:three_prime_UTR
CGATTCAGAGAGTCCTGTTG+AGG 0.644994 5_2:+24962509 None:intergenic
TTCGACGGCGTGATCGACGA+CGG 0.649107 5_2:+24962821 None:intergenic
GCTATTTGCACGGAATCACA+AGG 0.650119 5_2:-24960869 Msa0764660:intron
AGTGAATCAAAAGTAAGTTG+AGG 0.653725 5_2:+24960593 None:intergenic
GAGAACGTGTATGTCGGCGA+CGG 0.655422 5_2:+24962900 None:intergenic
AACAACGCGAGATTGAGCCA+AGG 0.662847 5_2:-24961028 Msa0764660:CDS
GTGGAGATTAGTGTGTTCGA+CGG 0.684024 5_2:+24962806 None:intergenic
TAGGTTACAGAGAAAAGCTG+AGG 0.684948 5_2:-24961722 Msa0764660:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAGTGACAATTTATTTTT+AGG - chr5_2:24960832-24960851 Msa0764660:intron 15.0%
!!! AAAACAATTTTTTTTGAGAT+AGG - chr5_2:24961263-24961282 Msa0764660:CDS 15.0%
!! TCAAACATATATAATATAGA+AGG + chr5_2:24961409-24961428 None:intergenic 15.0%
!! AACACAGTAAATAAAATTTA+AGG + chr5_2:24961731-24961750 None:intergenic 15.0%
!!! CTTTAATTGTATTGATTTAT+TGG - chr5_2:24961881-24961900 Msa0764660:intron 15.0%
!!! GATTATTATTTTAGTGAAAA+GGG - chr5_2:24962094-24962113 Msa0764660:intron 15.0%
!!! TCATTTTTTTTAACAAAACA+TGG + chr5_2:24962461-24962480 None:intergenic 15.0%
!! TAAAATAGCCAAATCAAAAA+AGG + chr5_2:24960111-24960130 None:intergenic 20.0%
!!! AATTTTTTTTTCTGTTTCGA+TGG - chr5_2:24960239-24960258 Msa0764660:three_prime_UTR 20.0%
!!! TTTGTCAAAGATTGTATTTT+TGG - chr5_2:24960355-24960374 Msa0764660:three_prime_UTR 20.0%
!!! TTGTCAAAGATTGTATTTTT+GGG - chr5_2:24960356-24960375 Msa0764660:three_prime_UTR 20.0%
!!! ATTACTGATTTTAATTTTGC+AGG - chr5_2:24960937-24960956 Msa0764660:three_prime_UTR 20.0%
!!! TTTAATACCATGATTTTGTT+TGG + chr5_2:24961206-24961225 None:intergenic 20.0%
!! TATATGTTAGAATAGAAGTT+TGG - chr5_2:24961338-24961357 Msa0764660:intron 20.0%
!!! ACTTTTGCGATTAAAATAAT+GGG + chr5_2:24961633-24961652 None:intergenic 20.0%
!!! AACTTTTGCGATTAAAATAA+TGG + chr5_2:24961634-24961653 None:intergenic 20.0%
!!! TTTTTATTATTTCCTTCTCT+AGG - chr5_2:24961658-24961677 Msa0764660:intron 20.0%
!!! GGATTATTATTTTAGTGAAA+AGG - chr5_2:24962093-24962112 Msa0764660:intron 20.0%
!!! TTATGCATTTTCTTTTTATG+AGG - chr5_2:24962260-24962279 Msa0764660:intron 20.0%
!! AATTATAAGCATAATTCAGA+TGG - chr5_2:24962294-24962313 Msa0764660:intron 20.0%
!!! GTTAATAGATAAAAAGTATG+TGG - chr5_2:24962503-24962522 Msa0764660:CDS 20.0%
!!! AAAAGTGCGTTATATAATTT+TGG - chr5_2:24962738-24962757 Msa0764660:intron 20.0%
!!! TTTTCTCTCCTTTTTTGATT+TGG - chr5_2:24960100-24960119 Msa0764660:three_prime_UTR 25.0%
! CGAAACAGAAAAAAAAATTC+TGG + chr5_2:24960238-24960257 None:intergenic 25.0%
!! CTTTTCCAGAAAATTACTTT+AGG - chr5_2:24960692-24960711 Msa0764660:three_prime_UTR 25.0%
!! GTGATTTTACTTTCATGATT+TGG - chr5_2:24960731-24960750 Msa0764660:three_prime_UTR 25.0%
! AGAATAGAAGCTACAAATAA+TGG - chr5_2:24961304-24961323 Msa0764660:intron 25.0%
!!! TCTCATGTTTGCTATTTTAT+CGG + chr5_2:24961475-24961494 None:intergenic 25.0%
! GAGAAGGAAATAATAAAAAG+AGG + chr5_2:24961657-24961676 None:intergenic 25.0%
!! AATCTCTATGATGTCATTTA+AGG - chr5_2:24961816-24961835 Msa0764660:CDS 25.0%
! AACCTAATAACATTTAGCAA+AGG + chr5_2:24962035-24962054 None:intergenic 25.0%
!! TTTTCTTTTTATGAGGTATG+TGG - chr5_2:24962267-24962286 Msa0764660:intron 25.0%
! ATGCATGTAAAGACAATAAT+GGG + chr5_2:24962362-24962381 None:intergenic 25.0%
! TATGCATGTAAAGACAATAA+TGG + chr5_2:24962363-24962382 None:intergenic 25.0%
! TTATTGTCTTTACATGCATA+TGG - chr5_2:24962363-24962382 Msa0764660:CDS 25.0%
!!! CATGTGCTTTAAGAAATTAT+AGG + chr5_2:24962557-24962576 None:intergenic 25.0%
!! TATATAACGCACTTTTAATG+TGG + chr5_2:24962734-24962753 None:intergenic 25.0%
CATGTGTTTATAATGCAGAT+TGG - chr5_2:24960274-24960293 Msa0764660:three_prime_UTR 30.0%
!!! TTTTTGGGTTCATGCAATTT+TGG - chr5_2:24960371-24960390 Msa0764660:three_prime_UTR 30.0%
! CAATTTTGGATTTTCACTAC+AGG - chr5_2:24960385-24960404 Msa0764660:three_prime_UTR 30.0%
!!! AATCAATTTTTTGACACCAG+TGG + chr5_2:24960510-24960529 None:intergenic 30.0%
AACAACATATCTCATTCACA+GGG + chr5_2:24960556-24960575 None:intergenic 30.0%
!! TATTTTTCAATTGCAGCCTA+TGG - chr5_2:24960598-24960617 Msa0764660:three_prime_UTR 30.0%
! AGAAGCCTAAAGTAATTTTC+TGG + chr5_2:24960700-24960719 None:intergenic 30.0%
!!! GATTTTAATTTTGCAGGTGT+TGG - chr5_2:24960943-24960962 Msa0764660:three_prime_UTR 30.0%
AAATTCTTTGTCAGAAACCT+TGG + chr5_2:24960996-24961015 None:intergenic 30.0%
AAAAGCTGAGGTAATGTTTA+TGG - chr5_2:24961096-24961115 Msa0764660:intron 30.0%
!! GATTTTGTTTGGCACATATA+TGG + chr5_2:24961195-24961214 None:intergenic 30.0%
ATATGTGCCAAACAAAATCA+TGG - chr5_2:24961196-24961215 Msa0764660:intron 30.0%
ACATAAAACAAACCTAGAGA+AGG + chr5_2:24961673-24961692 None:intergenic 30.0%
!!! TCTAGGTTTGTTTTATGTGA+AGG - chr5_2:24961675-24961694 Msa0764660:intron 30.0%
! TATGATGTTTTGCTTGATGA+TGG - chr5_2:24961702-24961721 Msa0764660:intron 30.0%
TAATAACATTTAGCAAAGGC+AGG + chr5_2:24962031-24962050 None:intergenic 30.0%
TGCCTTTGCTAAATGTTATT+AGG - chr5_2:24962030-24962049 Msa0764660:intron 30.0%
ATCCATTATATCTAGCACTT+TGG + chr5_2:24962077-24962096 None:intergenic 30.0%
AGTGAATCAAAAGTAAGTTG+AGG + chr5_2:24962216-24962235 None:intergenic 30.0%
AGAAAATGCATAACACCAAA+AGG + chr5_2:24962253-24962272 None:intergenic 30.0%
ACTTTCTTTCCTATTGCAAA+TGG - chr5_2:24962326-24962345 Msa0764660:CDS 30.0%
ATCTAATAGGAACATGCAAA+TGG + chr5_2:24962406-24962425 None:intergenic 30.0%
!! TTTACATTGACTCTATTCCT+TGG + chr5_2:24962432-24962451 None:intergenic 30.0%
!!! CGTTGATTTGATTGAAGTTT+TGG - chr5_2:24962645-24962664 Msa0764660:intron 30.0%
CTTAATGCAAAGTAGAATCA+TGG - chr5_2:24962668-24962687 Msa0764660:intron 30.0%
AAGTAGAATCATGGACAATT+TGG - chr5_2:24962677-24962696 Msa0764660:intron 30.0%
!! TTTGGTGTTCAAGTTGAAAT+AGG - chr5_2:24962695-24962714 Msa0764660:intron 30.0%
!! TTGGTGTTCAAGTTGAAATA+GGG - chr5_2:24962696-24962715 Msa0764660:intron 30.0%
! TGTGCTTTTAGTCAATTACA+TGG + chr5_2:24962855-24962874 None:intergenic 30.0%
TACAGTTTGTAATTGATGCA+AGG - chr5_2:24962910-24962929 Msa0764660:intron 30.0%
!!! GTTTTAGAGAACGTGTATGT+CGG + chr5_2:24959915-24959934 None:intergenic 35.0%
ATGCTGAAAAAGATGGTGAA+CGG + chr5_2:24960025-24960044 None:intergenic 35.0%
TTTGATCGATTGAGAGGATT+GGG - chr5_2:24960142-24960161 Msa0764660:three_prime_UTR 35.0%
TTTCTGTTTCGATGGTGATT+AGG - chr5_2:24960247-24960266 Msa0764660:three_prime_UTR 35.0%
TTCTGTTTCGATGGTGATTA+GGG - chr5_2:24960248-24960267 Msa0764660:three_prime_UTR 35.0%
CAAAAAAAACCCATACCAGT+TGG + chr5_2:24960326-24960345 None:intergenic 35.0%
! ATTTTCACTACAGGATCTGT+TGG - chr5_2:24960394-24960413 Msa0764660:three_prime_UTR 35.0%
!! GGCATTTCAAGTTGCAATAA+TGG - chr5_2:24960418-24960437 Msa0764660:three_prime_UTR 35.0%
!! CAAGTTGCAATAATGGATAC+AGG - chr5_2:24960425-24960444 Msa0764660:three_prime_UTR 35.0%
GAACAACATATCTCATTCAC+AGG + chr5_2:24960557-24960576 None:intergenic 35.0%
!! ATCAACTCCTTTAGAACCAT+AGG + chr5_2:24960617-24960636 None:intergenic 35.0%
!! GTTCTAAAGGAGTTGATACA+TGG - chr5_2:24960620-24960639 Msa0764660:three_prime_UTR 35.0%
ATACATGGAATTTGAAGAGC+TGG - chr5_2:24960635-24960654 Msa0764660:three_prime_UTR 35.0%
!!! TGGAGTATTGATCGCTTTTT+AGG - chr5_2:24960751-24960770 Msa0764660:three_prime_UTR 35.0%
!!! GGAGTATTGATCGCTTTTTA+GGG - chr5_2:24960752-24960771 Msa0764660:three_prime_UTR 35.0%
TGACTACAGAGTTACTTATG+AGG - chr5_2:24961020-24961039 Msa0764660:CDS 35.0%
ACTTATGAGGAAATTGCTGA+AGG - chr5_2:24961033-24961052 Msa0764660:CDS 35.0%
CATGAGACAATCATGTGATT+TGG - chr5_2:24961488-24961507 Msa0764660:intron 35.0%
TGCATTTGATTGCTCTGTTT+CGG - chr5_2:24961543-24961562 Msa0764660:intron 35.0%
TAATGACTAATTCACAGACC+TGG + chr5_2:24961604-24961623 None:intergenic 35.0%
TTTAGATCAATTGTCTCCCT+TGG + chr5_2:24961798-24961817 None:intergenic 35.0%
! GACCAAAGTGCTAGATATAA+TGG - chr5_2:24962072-24962091 Msa0764660:intron 35.0%
! TTTGACACCTTTTGTCCTTT+TGG - chr5_2:24962235-24962254 Msa0764660:intron 35.0%
CATAACACCAAAAGGACAAA+AGG + chr5_2:24962245-24962264 None:intergenic 35.0%
! ATGAATCTGCCATTTGCAAT+AGG + chr5_2:24962338-24962357 None:intergenic 35.0%
TATTCCTTGGCTCATCTAAT+AGG + chr5_2:24962419-24962438 None:intergenic 35.0%
!! TGGTGTTCAAGTTGAAATAG+GGG - chr5_2:24962697-24962716 Msa0764660:intron 35.0%
! ATAACGCACTTTTAATGTGG+AGG + chr5_2:24962731-24962750 None:intergenic 35.0%
CTTAGACATAGTAAGCTTAC+AGG + chr5_2:24962817-24962836 None:intergenic 35.0%
CAATTACATGGTAAGCTTAC+AGG + chr5_2:24962843-24962862 None:intergenic 35.0%
! TTGACTAAAAGCACATCACA+AGG - chr5_2:24962860-24962879 Msa0764660:intron 35.0%
GACATGCATGCTGAAAAAGA+TGG + chr5_2:24960032-24960051 None:intergenic 40.0%
!!! CTTTTTCAGCATGCATGTCT+AGG - chr5_2:24960033-24960052 Msa0764660:three_prime_UTR 40.0%
TTCCTCTTTGATCGATTGAG+AGG - chr5_2:24960136-24960155 Msa0764660:three_prime_UTR 40.0%
ATCCTCTCAATCGATCAAAG+AGG + chr5_2:24960141-24960160 None:intergenic 40.0%
CTTTGATCGATTGAGAGGAT+TGG - chr5_2:24960141-24960160 Msa0764660:three_prime_UTR 40.0%
GTTCTGCTTATCAAAGAGAC+TGG - chr5_2:24960470-24960489 Msa0764660:three_prime_UTR 40.0%
! AAGAGACTGGTCAAGCTATT+AGG - chr5_2:24960483-24960502 Msa0764660:three_prime_UTR 40.0%
! ATTGTTTTCTTAGCTCCCAC+TGG - chr5_2:24960527-24960546 Msa0764660:three_prime_UTR 40.0%
!! ATTGCAGCCTATGGTTCTAA+AGG - chr5_2:24960607-24960626 Msa0764660:three_prime_UTR 40.0%
!!! AATTTTGCAGGTGTTGGTCT+TGG - chr5_2:24960949-24960968 Msa0764660:three_prime_UTR 40.0%
TATGAGGAAATTGCTGAAGG+TGG - chr5_2:24961036-24961055 Msa0764660:CDS 40.0%
TAGGTTACAGAGAAAAGCTG+AGG - chr5_2:24961084-24961103 Msa0764660:intron 40.0%
GCATTTGATTGCTCTGTTTC+GGG - chr5_2:24961544-24961563 Msa0764660:intron 40.0%
! AGGTTCTGATCTTCAATCAG+TGG - chr5_2:24962050-24962069 Msa0764660:intron 40.0%
AAAAGGGTTTCGAAGTTTCC+AGG - chr5_2:24962110-24962129 Msa0764660:intron 40.0%
TTCGAAGTTTCCAGGATTGA+CGG - chr5_2:24962118-24962137 Msa0764660:intron 40.0%
TGAAGCTGGATGATAGGAAA+CGG - chr5_2:24962146-24962165 Msa0764660:intron 40.0%
TGTTCCTATTAGATGAGCCA+AGG - chr5_2:24962412-24962431 Msa0764660:intron 40.0%
AAAGCACATGCTCTTCTATC+TGG - chr5_2:24962567-24962586 Msa0764660:CDS 40.0%
AGTGAGAACAACAGTTTGTG+TGG - chr5_2:24962966-24962985 Msa0764660:five_prime_UTR 40.0%
GTGGAGATTAGTGTGTTCGA+CGG + chr5_2:24960003-24960022 None:intergenic 45.0%
CTGAAAAAGATGGTGAACGG+TGG + chr5_2:24960022-24960041 None:intergenic 45.0%
!! TAATGCAGATTGGCCTCAAC+AGG - chr5_2:24960284-24960303 Msa0764660:three_prime_UTR 45.0%
TTCACTACAGGATCTGTTGG+AGG - chr5_2:24960397-24960416 Msa0764660:three_prime_UTR 45.0%
GAATTTGAAGAGCTGGCAGA+AGG - chr5_2:24960642-24960661 Msa0764660:three_prime_UTR 45.0%
!!! TGATCGCTTTTTAGGGCTCA+TGG - chr5_2:24960759-24960778 Msa0764660:intron 45.0%
AGGAAATTGCTGAAGGTGGA+TGG - chr5_2:24961040-24961059 Msa0764660:CDS 45.0%
GCTGAAGGTGGATGGTATTA+AGG - chr5_2:24961048-24961067 Msa0764660:CDS 45.0%
GTGGATGGTATTAAGGCTGA+AGG - chr5_2:24961055-24961074 Msa0764660:intron 45.0%
TTAAGGCTGAAGGTGATGCT+AGG - chr5_2:24961065-24961084 Msa0764660:intron 45.0%
! GCGTTGTTGGTTGATGTTGT+AGG + chr5_2:24961766-24961785 None:intergenic 45.0%
GCTATTTGCACGGAATCACA+AGG - chr5_2:24961937-24961956 Msa0764660:intron 45.0%
! GCCTTTCTGCTTCCTTTTTG+TGG - chr5_2:24961984-24962003 Msa0764660:intron 45.0%
GCCACAAAAAGGAAGCAGAA+AGG + chr5_2:24961988-24962007 None:intergenic 45.0%
AGTTTCCAGGATTGACGGTT+CGG - chr5_2:24962123-24962142 Msa0764660:intron 45.0%
!!! ATTTTATTAAAAAAATTGAT+TGG - chr5_2:24962622-24962641 Msa0764660:intron 5.0%
ACAGTGAAGATCGCAGGTGA+CGG + chr5_2:24959872-24959891 None:intergenic 50.0%
ACAGTGAAGATCGCAGGTGA+CGG + chr5_2:24959872-24959891 None:intergenic 50.0%
CGATTCAGAGAGTCCTGTTG+AGG + chr5_2:24960300-24960319 None:intergenic 50.0%
TGAATCGCGTCCAACTGGTA+TGG - chr5_2:24960313-24960332 Msa0764660:three_prime_UTR 50.0%
GAATCGCGTCCAACTGGTAT+GGG - chr5_2:24960314-24960333 Msa0764660:three_prime_UTR 50.0%
GGTCAAGCTATTAGGTCCAC+TGG - chr5_2:24960491-24960510 Msa0764660:three_prime_UTR 50.0%
TCATTCACAGGGTGACCAGT+GGG + chr5_2:24960545-24960564 None:intergenic 50.0%
GTCTTGGATGTGGCTGTCAA+TGG - chr5_2:24960965-24960984 Msa0764660:three_prime_UTR 50.0%
TTGGATGTGGCTGTCAATGG+AGG - chr5_2:24960968-24960987 Msa0764660:three_prime_UTR 50.0%
TGGATGTGGCTGTCAATGGA+GGG - chr5_2:24960969-24960988 Msa0764660:three_prime_UTR 50.0%
TTGGCTCAATCTCGCGTTGT+TGG + chr5_2:24961779-24961798 None:intergenic 50.0%
AACAACGCGAGATTGAGCCA+AGG - chr5_2:24961778-24961797 Msa0764660:CDS 50.0%
ACAACGCGAGATTGAGCCAA+GGG - chr5_2:24961779-24961798 Msa0764660:CDS 50.0%
! TCAATTGCTCGACCGATTCC+TGG - chr5_2:24961910-24961929 Msa0764660:intron 50.0%
CAAATAGCCGCTCCAGGAAT+CGG + chr5_2:24961925-24961944 None:intergenic 50.0%
GTAGCAGCTTGGCCACAAAA+AGG + chr5_2:24961999-24962018 None:intergenic 50.0%
! GTTCGGTGAAGCTGGATGAT+AGG - chr5_2:24962140-24962159 Msa0764660:intron 50.0%
GGCAGGTCTGTATCAATGCT+TGG - chr5_2:24962167-24962186 Msa0764660:CDS 50.0%
GAGAACGTGTATGTCGGCGA+CGG + chr5_2:24959909-24959928 None:intergenic 55.0%
CTCTCTGAATCGCGTCCAAC+TGG - chr5_2:24960308-24960327 Msa0764660:three_prime_UTR 55.0%
CTCATTCACAGGGTGACCAG+TGG + chr5_2:24960546-24960565 None:intergenic 55.0%
!! GCAGGTGTTGGTCTTGGATG+TGG - chr5_2:24960955-24960974 Msa0764660:three_prime_UTR 55.0%
!! CTGTTTCGGGTTGACTTCGC+AGG - chr5_2:24961557-24961576 Msa0764660:intron 55.0%
TCCTGGAGCGGCTATTTGCA+CGG - chr5_2:24961927-24961946 Msa0764660:intron 55.0%
GGTGCAGATGAGTAGCAGCT+TGG + chr5_2:24962010-24962029 None:intergenic 55.0%
CTTCACCGAACCGTCAATCC+TGG + chr5_2:24962131-24962150 None:intergenic 55.0%
!! GATTGACGGTTCGGTGAAGC+TGG - chr5_2:24962132-24962151 Msa0764660:intron 55.0%
GCTGGATGATAGGAAACGGC+AGG - chr5_2:24962150-24962169 Msa0764660:CDS 55.0%
ACGGCGACAGTGAAGATCGC+AGG + chr5_2:24959878-24959897 None:intergenic 60.0%
ACGGCGACAGTGAAGATCGC+AGG + chr5_2:24959878-24959897 None:intergenic 60.0%
GACGGCGACGACGAGATTGA+CGG + chr5_2:24959970-24959989 None:intergenic 60.0%
TTCGACGGCGTGATCGACGA+CGG + chr5_2:24959988-24960007 None:intergenic 60.0%
GGCTGTCAATGGAGGGACCA+AGG - chr5_2:24960976-24960995 Msa0764660:three_prime_UTR 60.0%
! CGGGTTGACTTCGCAGGAAG+AGG - chr5_2:24961563-24961582 Msa0764660:intron 60.0%
! TGCTCGACCGATTCCTGGAG+CGG - chr5_2:24961915-24961934 Msa0764660:intron 60.0%
TCCGTGCAAATAGCCGCTCC+AGG + chr5_2:24961931-24961950 None:intergenic 60.0%
GTCGGCGACGGCGAGATTGA+CGG + chr5_2:24959897-24959916 None:intergenic 65.0%
!! AGGAGAGCTTGCTGAGCGCC+AGG - chr5_2:24961583-24961602 Msa0764660:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 24959832 24962996 24959832 ID=Msa0764660;Name=Msa0764660
chr5_2 mRNA 24959832 24962996 24959832 ID=Msa0764660-mRNA-1;Parent=Msa0764660;Name=Msa0764660-mRNA-1;_AED=0.04;_eAED=0.04;_QI=142|0.87|0.77|1|0.5|0.55|9|1087|181
chr5_2 exon 24959832 24960776 24959832 ID=Msa0764660-mRNA-1:exon:10081;Parent=Msa0764660-mRNA-1
chr5_2 exon 24960870 24961071 24960870 ID=Msa0764660-mRNA-1:exon:10080;Parent=Msa0764660-mRNA-1
chr5_2 exon 24961224 24961289 24961224 ID=Msa0764660-mRNA-1:exon:10079;Parent=Msa0764660-mRNA-1
chr5_2 exon 24961723 24961869 24961723 ID=Msa0764660-mRNA-1:exon:10078;Parent=Msa0764660-mRNA-1
chr5_2 exon 24962147 24962214 24962147 ID=Msa0764660-mRNA-1:exon:10077;Parent=Msa0764660-mRNA-1
chr5_2 exon 24962316 24962421 24962316 ID=Msa0764660-mRNA-1:exon:10076;Parent=Msa0764660-mRNA-1
chr5_2 exon 24962499 24962589 24962499 ID=Msa0764660-mRNA-1:exon:10075;Parent=Msa0764660-mRNA-1
chr5_2 exon 24962774 24962840 24962774 ID=Msa0764660-mRNA-1:exon:10074;Parent=Msa0764660-mRNA-1
chr5_2 exon 24962914 24962996 24962914 ID=Msa0764660-mRNA-1:exon:10073;Parent=Msa0764660-mRNA-1
chr5_2 five_prime_UTR 24962914 24962996 24962914 ID=Msa0764660-mRNA-1:five_prime_utr;Parent=Msa0764660-mRNA-1
chr5_2 five_prime_UTR 24962782 24962840 24962782 ID=Msa0764660-mRNA-1:five_prime_utr;Parent=Msa0764660-mRNA-1
chr5_2 CDS 24962774 24962781 24962774 ID=Msa0764660-mRNA-1:cds;Parent=Msa0764660-mRNA-1
chr5_2 CDS 24962499 24962589 24962499 ID=Msa0764660-mRNA-1:cds;Parent=Msa0764660-mRNA-1
chr5_2 CDS 24962316 24962421 24962316 ID=Msa0764660-mRNA-1:cds;Parent=Msa0764660-mRNA-1
chr5_2 CDS 24962147 24962214 24962147 ID=Msa0764660-mRNA-1:cds;Parent=Msa0764660-mRNA-1
chr5_2 CDS 24961723 24961869 24961723 ID=Msa0764660-mRNA-1:cds;Parent=Msa0764660-mRNA-1
chr5_2 CDS 24961224 24961289 24961224 ID=Msa0764660-mRNA-1:cds;Parent=Msa0764660-mRNA-1
chr5_2 CDS 24961012 24961071 24961012 ID=Msa0764660-mRNA-1:cds;Parent=Msa0764660-mRNA-1
chr5_2 three_prime_UTR 24960870 24961011 24960870 ID=Msa0764660-mRNA-1:three_prime_utr;Parent=Msa0764660-mRNA-1
chr5_2 three_prime_UTR 24959832 24960776 24959832 ID=Msa0764660-mRNA-1:three_prime_utr;Parent=Msa0764660-mRNA-1
Gene Sequence

>Msa0764660

ATGTCTAGAATTTTTTTTTCTGTTTCGATGGTGATTAGGGTTTCATGTGTTTATAATGCAGATTGGCCTCAACAGGACTCTCTGAATCGCGTCCAACTGGATCTGTTGGAGGCATTTCAAGTTGCAATAATGGATACAGGTTCAAGAAAAACATTGATTTCTGTTCTGCTTATCAAAGAGACTGGTCAAGCTATTAGGTCCACTGCCTATGGTTCTAAAGGAGTTGATACATGGAATTTGAAGAGCTGGCAGAAGGAAATTAGTGACAATGATGTGTTGGTCTTGGATGTGGCTGTCAATGGAGGGACCAAGGTTTCTGACAAAGAATTTGTTGTGACTACAGAGTTACTTATGAGGAAATTGCTGAAGGTGGATGGTATTAAGGCTGAAGGTGATGCTAGGTTACAGAGAAAAGCTGAGATTGTGCATTTGATTGCTCTGTTTCGGGTTGACTTCGCAGGAAGAGGAGAGCTTGCTGAGCGCCAGATAATACCTACAACATCAACCAACAACGCGAGATTGAGCCAAGGGAGACAATTGATCTAA
Protein sequence

>Msa0764660

MSRIFFSVSMVIRVSCVYNADWPQQDSLNRVQLDLLEAFQVAIMDTGSRKTLISVLLIKETGQAIRSTAYGSKGVDTWNLKSWQKEISDNDVLVLDVAVNGGTKVSDKEFVVTTELLMRKLLKVDGIKAEGDARLQRKAEIVHLIALFRVDFAGRGELAERQIIPTTSTNNARLSQGRQLI*