AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0337510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337510 MtrunA17_Chr2g0328391 89.583 96 10 0 2 97 254 349 6.61e-52 173
Msa0337510 MtrunA17_Chr4g0031711 63.889 72 26 0 26 97 340 411 2.76e-28 107
Msa0337510 MtrunA17_Chr4g0031721 56.522 92 38 1 7 96 274 365 1.88e-26 102
Msa0337510 MtrunA17_Chr4g0031701 62.857 70 26 0 28 97 235 304 6.49e-25 98.2
Msa0337510 MtrunA17_Chr7g0238891 57.500 80 34 0 29 108 354 433 2.10e-24 96.7
Msa0337510 MtrunA17_Chr4g0031731 61.429 70 27 0 28 97 235 304 2.26e-24 96.7
Msa0337510 MtrunA17_Chr2g0328381 58.462 65 27 0 32 96 260 324 9.63e-22 89.0
Msa0337510 MtrunA17_Chr2g0328361 57.812 64 27 0 33 96 260 323 3.99e-21 87.4
Msa0337510 MtrunA17_Chr2g0328341 60.317 63 22 1 37 96 259 321 5.10e-20 84.3
Msa0337510 MtrunA17_Chr2g0328401 56.338 71 29 1 27 95 268 338 4.83e-18 78.6
Msa0337510 MtrunA17_Chr4g0031741 63.636 55 20 0 42 96 279 333 1.89e-17 77.0
Msa0337510 MtrunA17_Chr2g0328411 50.649 77 31 2 27 96 262 338 4.84e-16 72.8
Msa0337510 MtrunA17_Chr2g0328431 49.351 77 32 1 27 96 262 338 8.63e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337510 AT1G07530.1 50.000 88 42 1 11 96 358 445 2.36e-22 91.3
Msa0337510 AT1G07520.1 61.765 68 23 1 29 96 303 367 1.35e-20 86.3
Msa0337510 AT1G07520.3 61.765 68 23 1 29 96 304 368 1.35e-20 86.3
Msa0337510 AT1G07520.2 61.765 68 23 1 29 96 139 203 1.76e-20 85.9
Msa0337510 AT1G07520.4 61.765 68 23 1 29 96 139 203 1.76e-20 85.9
Msa0337510 AT2G37650.1 52.703 74 35 0 35 108 335 408 1.83e-19 83.2
Msa0337510 AT2G37650.2 52.703 74 35 0 35 108 323 396 1.95e-19 82.8
Msa0337510 AT2G29060.1 48.718 78 40 0 27 104 301 378 4.67e-19 81.6
Msa0337510 AT2G29060.2 48.718 78 40 0 27 104 264 341 4.69e-19 81.6
Msa0337510 AT2G29065.1 62.500 56 21 0 41 96 246 301 2.87e-15 70.9
Msa0337510 AT5G59450.1 40.860 93 50 2 7 96 186 276 6.90e-15 70.1
Msa0337510 AT3G46600.3 47.826 69 36 0 28 96 161 229 4.10e-14 67.8
Msa0337510 AT3G46600.1 47.826 69 36 0 28 96 193 261 4.21e-14 67.8
Msa0337510 AT3G46600.2 52.830 53 25 0 44 96 1 53 7.35e-12 61.2

Find 30 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGACTATGACAATGTAAT+TGG 0.287106 2_4:-57548569 Msa0337510:CDS
AGGAAGCATTCTTGTCCTTC+AGG 0.331779 2_4:-57549120 Msa0337510:CDS
TTTCTGCTTACTTTCAGATA+TGG 0.359746 2_4:+57548539 None:intergenic
TGTGGATTTGAGGACCCTTT+TGG 0.385294 2_4:-57549208 Msa0337510:CDS
ATGAATTGTTGAAGCAGAAT+AGG 0.424251 2_4:-57549140 Msa0337510:CDS
CTGTGTCTGCTAATGACAAT+AGG 0.426533 2_4:-57549170 Msa0337510:CDS
AGAGGGATGTTGTGGATTTG+AGG 0.451718 2_4:-57549218 Msa0337510:CDS
ACACAGGTTGGTGAACTTGA+TGG 0.468419 2_4:-57549273 Msa0337510:CDS
CGAAGAAACAAGGGAGGAAG+AGG 0.489890 2_4:-57549236 Msa0337510:CDS
AGGGAGGAAGAGGGATGTTG+TGG 0.530372 2_4:-57549226 Msa0337510:CDS
AGGTGATGCATCACAGAGAA+TGG 0.535230 2_4:-57549100 Msa0337510:CDS
GTCGTTGCGTGCTGAACAAA+AGG 0.535793 2_4:-57549319 Msa0337510:CDS
CTCTGTGATGCATCACCTGA+AGG 0.537655 2_4:+57549105 None:intergenic
CAAAAGGATGGATCAGTGGT+GGG 0.540630 2_4:-57549303 Msa0337510:CDS
CTTGTGCACACAGAACCAAA+AGG 0.550869 2_4:+57549193 None:intergenic
TTGTGCACACAGAACCAAAA+GGG 0.577974 2_4:+57549194 None:intergenic
TTGCGTGCTGAACAAAAGGA+TGG 0.589695 2_4:-57549315 Msa0337510:CDS
TGGTGAACTTGATGGAGGGA+AGG 0.605242 2_4:-57549265 Msa0337510:CDS
ACAAAAGGATGGATCAGTGG+TGG 0.605810 2_4:-57549304 Msa0337510:CDS
GAATGCGATTGAAGCGCGCA+TGG 0.609128 2_4:-57549064 Msa0337510:intron
AAGGCACGTTCGAAGAAACA+AGG 0.620215 2_4:-57549246 Msa0337510:CDS
CAGGTTGGTGAACTTGATGG+AGG 0.633962 2_4:-57549270 Msa0337510:CDS
AAAAGGATGGATCAGTGGTG+GGG 0.638533 2_4:-57549302 Msa0337510:CDS
AGGTTGGTGAACTTGATGGA+GGG 0.640702 2_4:-57549269 Msa0337510:CDS
GAAGAAACAAGGGAGGAAGA+GGG 0.647199 2_4:-57549235 Msa0337510:CDS
AGTGGTGGGGAGTAGAACAC+AGG 0.661039 2_4:-57549289 Msa0337510:CDS
TGAACAAAAGGATGGATCAG+TGG 0.692488 2_4:-57549307 Msa0337510:CDS
GTGGGGAGTAGAACACAGGT+TGG 0.695448 2_4:-57549285 Msa0337510:CDS
CACGTTCGAAGAAACAAGGG+AGG 0.709283 2_4:-57549242 Msa0337510:CDS
AGGCACGTTCGAAGAAACAA+GGG 0.717525 2_4:-57549245 Msa0337510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATGTTGTTTTGTAAAT+AGG - chr2_4:57549171-57549190 Msa0337510:CDS 15.0%
!!! ATATATGTTGTTTTGTAAAT+AGG - chr2_4:57549171-57549190 Msa0337510:CDS 15.0%
!! CATATATAATATTTGTGTAC+TGG + chr2_4:57548833-57548852 None:intergenic 20.0%
!!! AATGTGCAAATTTTTTGAAA+GGG + chr2_4:57548918-57548937 None:intergenic 20.0%
!!! AAATGTGCAAATTTTTTGAA+AGG + chr2_4:57548919-57548938 None:intergenic 20.0%
!!! TTTAAAAAACATGGCAATTT+TGG + chr2_4:57549011-57549030 None:intergenic 20.0%
!!! ACATACATCTTTAAAAAACA+TGG + chr2_4:57549020-57549039 None:intergenic 20.0%
!!! GTTTTTTAAAGATGTATGTT+TGG - chr2_4:57549021-57549040 Msa0337510:intron 20.0%
!! CATATATAATATTTGTGTAC+TGG + chr2_4:57548833-57548852 None:intergenic 20.0%
!!! AATGTGCAAATTTTTTGAAA+GGG + chr2_4:57548918-57548937 None:intergenic 20.0%
!!! AAATGTGCAAATTTTTTGAA+AGG + chr2_4:57548919-57548938 None:intergenic 20.0%
!!! TTTAAAAAACATGGCAATTT+TGG + chr2_4:57549011-57549030 None:intergenic 20.0%
!!! ACATACATCTTTAAAAAACA+TGG + chr2_4:57549020-57549039 None:intergenic 20.0%
!!! GTTTTTTAAAGATGTATGTT+TGG - chr2_4:57549021-57549040 Msa0337510:intron 20.0%
! TTAAAGATGTATGTTTGGAT+TGG - chr2_4:57549026-57549045 Msa0337510:intron 25.0%
! ATAATTGTGTTTGTTCATGA+AGG - chr2_4:57549077-57549096 Msa0337510:CDS 25.0%
! TAGGAAACTTCTATTTGATT+AGG - chr2_4:57549190-57549209 Msa0337510:CDS 25.0%
! GTTAGAGATGAGAAAATTAA+GGG + chr2_4:57549233-57549252 None:intergenic 25.0%
! AGTTAGAGATGAGAAAATTA+AGG + chr2_4:57549234-57549253 None:intergenic 25.0%
!!! TCTCTAACTTTTGTTTATTG+TGG - chr2_4:57549245-57549264 Msa0337510:CDS 25.0%
! TTAAAGATGTATGTTTGGAT+TGG - chr2_4:57549026-57549045 Msa0337510:intron 25.0%
! ATAATTGTGTTTGTTCATGA+AGG - chr2_4:57549077-57549096 Msa0337510:CDS 25.0%
! TAGGAAACTTCTATTTGATT+AGG - chr2_4:57549190-57549209 Msa0337510:CDS 25.0%
! GTTAGAGATGAGAAAATTAA+GGG + chr2_4:57549233-57549252 None:intergenic 25.0%
! AGTTAGAGATGAGAAAATTA+AGG + chr2_4:57549234-57549253 None:intergenic 25.0%
!!! TCTCTAACTTTTGTTTATTG+TGG - chr2_4:57549245-57549264 Msa0337510:CDS 25.0%
ATGAATTGTTGAAGCAGAAT+AGG - chr2_4:57548719-57548738 Msa0337510:intron 30.0%
! CAGCAACATTAAACATCTTT+TGG + chr2_4:57548858-57548877 None:intergenic 30.0%
!! AAGCTAAGTTGATGTATCAT+AGG + chr2_4:57549117-57549136 None:intergenic 30.0%
AGTGACTATGACAATGTAAT+TGG - chr2_4:57549290-57549309 Msa0337510:CDS 30.0%
TTTCTGCTTACTTTCAGATA+TGG + chr2_4:57549323-57549342 None:intergenic 30.0%
ATGAATTGTTGAAGCAGAAT+AGG - chr2_4:57548719-57548738 Msa0337510:intron 30.0%
! CAGCAACATTAAACATCTTT+TGG + chr2_4:57548858-57548877 None:intergenic 30.0%
!! AAGCTAAGTTGATGTATCAT+AGG + chr2_4:57549117-57549136 None:intergenic 30.0%
AGTGACTATGACAATGTAAT+TGG - chr2_4:57549290-57549309 Msa0337510:CDS 30.0%
TTTCTGCTTACTTTCAGATA+TGG + chr2_4:57549323-57549342 None:intergenic 30.0%
TTGTGTTTGTTCATGAAGGT+TGG - chr2_4:57549081-57549100 Msa0337510:CDS 35.0%
TTGTGTTTGTTCATGAAGGT+TGG - chr2_4:57549081-57549100 Msa0337510:CDS 35.0%
TGAACAAAAGGATGGATCAG+TGG - chr2_4:57548552-57548571 Msa0337510:CDS 40.0%
TTGTGCACACAGAACCAAAA+GGG + chr2_4:57548668-57548687 None:intergenic 40.0%
CTGTGTCTGCTAATGACAAT+AGG - chr2_4:57548689-57548708 Msa0337510:intron 40.0%
GGCAAGCAGAGATAAAAACT+TGG + chr2_4:57548897-57548916 None:intergenic 40.0%
TGAACAAAAGGATGGATCAG+TGG - chr2_4:57548552-57548571 Msa0337510:CDS 40.0%
TTGTGCACACAGAACCAAAA+GGG + chr2_4:57548668-57548687 None:intergenic 40.0%
CTGTGTCTGCTAATGACAAT+AGG - chr2_4:57548689-57548708 Msa0337510:intron 40.0%
GGCAAGCAGAGATAAAAACT+TGG + chr2_4:57548897-57548916 None:intergenic 40.0%
TTGCGTGCTGAACAAAAGGA+TGG - chr2_4:57548544-57548563 Msa0337510:CDS 45.0%
ACAAAAGGATGGATCAGTGG+TGG - chr2_4:57548555-57548574 Msa0337510:CDS 45.0%
CAAAAGGATGGATCAGTGGT+GGG - chr2_4:57548556-57548575 Msa0337510:CDS 45.0%
AAAAGGATGGATCAGTGGTG+GGG - chr2_4:57548557-57548576 Msa0337510:CDS 45.0%
!! ACACAGGTTGGTGAACTTGA+TGG - chr2_4:57548586-57548605 Msa0337510:CDS 45.0%
!! AGGTTGGTGAACTTGATGGA+GGG - chr2_4:57548590-57548609 Msa0337510:intron 45.0%
AAGGCACGTTCGAAGAAACA+AGG - chr2_4:57548613-57548632 Msa0337510:intron 45.0%
AGGCACGTTCGAAGAAACAA+GGG - chr2_4:57548614-57548633 Msa0337510:intron 45.0%
GAAGAAACAAGGGAGGAAGA+GGG - chr2_4:57548624-57548643 Msa0337510:intron 45.0%
AGAGGGATGTTGTGGATTTG+AGG - chr2_4:57548641-57548660 Msa0337510:intron 45.0%
! TGTGGATTTGAGGACCCTTT+TGG - chr2_4:57548651-57548670 Msa0337510:intron 45.0%
CTTGTGCACACAGAACCAAA+AGG + chr2_4:57548669-57548688 None:intergenic 45.0%
!! AGGAAGCATTCTTGTCCTTC+AGG - chr2_4:57548739-57548758 Msa0337510:intron 45.0%
AGGTGATGCATCACAGAGAA+TGG - chr2_4:57548759-57548778 Msa0337510:intron 45.0%
TTGCGTGCTGAACAAAAGGA+TGG - chr2_4:57548544-57548563 Msa0337510:CDS 45.0%
ACAAAAGGATGGATCAGTGG+TGG - chr2_4:57548555-57548574 Msa0337510:CDS 45.0%
CAAAAGGATGGATCAGTGGT+GGG - chr2_4:57548556-57548575 Msa0337510:CDS 45.0%
AAAAGGATGGATCAGTGGTG+GGG - chr2_4:57548557-57548576 Msa0337510:CDS 45.0%
!! ACACAGGTTGGTGAACTTGA+TGG - chr2_4:57548586-57548605 Msa0337510:CDS 45.0%
!! AGGTTGGTGAACTTGATGGA+GGG - chr2_4:57548590-57548609 Msa0337510:intron 45.0%
AAGGCACGTTCGAAGAAACA+AGG - chr2_4:57548613-57548632 Msa0337510:intron 45.0%
AGGCACGTTCGAAGAAACAA+GGG - chr2_4:57548614-57548633 Msa0337510:intron 45.0%
GAAGAAACAAGGGAGGAAGA+GGG - chr2_4:57548624-57548643 Msa0337510:intron 45.0%
AGAGGGATGTTGTGGATTTG+AGG - chr2_4:57548641-57548660 Msa0337510:intron 45.0%
! TGTGGATTTGAGGACCCTTT+TGG - chr2_4:57548651-57548670 Msa0337510:intron 45.0%
CTTGTGCACACAGAACCAAA+AGG + chr2_4:57548669-57548688 None:intergenic 45.0%
!! AGGAAGCATTCTTGTCCTTC+AGG - chr2_4:57548739-57548758 Msa0337510:intron 45.0%
AGGTGATGCATCACAGAGAA+TGG - chr2_4:57548759-57548778 Msa0337510:intron 45.0%
!! AAAAAAAATATATTGAAATA+TGG + chr2_4:57549140-57549159 None:intergenic 5.0%
!!! TTCAATATATTTTTTTTTTA+TGG - chr2_4:57549143-57549162 Msa0337510:CDS 5.0%
!! AAAAAAAATATATTGAAATA+TGG + chr2_4:57549140-57549159 None:intergenic 5.0%
!!! TTCAATATATTTTTTTTTTA+TGG - chr2_4:57549143-57549162 Msa0337510:CDS 5.0%
GTCGTTGCGTGCTGAACAAA+AGG - chr2_4:57548540-57548559 Msa0337510:CDS 50.0%
!! CAGGTTGGTGAACTTGATGG+AGG - chr2_4:57548589-57548608 Msa0337510:intron 50.0%
TGGTGAACTTGATGGAGGGA+AGG - chr2_4:57548594-57548613 Msa0337510:intron 50.0%
CACGTTCGAAGAAACAAGGG+AGG - chr2_4:57548617-57548636 Msa0337510:intron 50.0%
CGAAGAAACAAGGGAGGAAG+AGG - chr2_4:57548623-57548642 Msa0337510:intron 50.0%
CTCTGTGATGCATCACCTGA+AGG + chr2_4:57548757-57548776 None:intergenic 50.0%
GTCGTTGCGTGCTGAACAAA+AGG - chr2_4:57548540-57548559 Msa0337510:CDS 50.0%
!! CAGGTTGGTGAACTTGATGG+AGG - chr2_4:57548589-57548608 Msa0337510:intron 50.0%
TGGTGAACTTGATGGAGGGA+AGG - chr2_4:57548594-57548613 Msa0337510:intron 50.0%
CACGTTCGAAGAAACAAGGG+AGG - chr2_4:57548617-57548636 Msa0337510:intron 50.0%
CGAAGAAACAAGGGAGGAAG+AGG - chr2_4:57548623-57548642 Msa0337510:intron 50.0%
CTCTGTGATGCATCACCTGA+AGG + chr2_4:57548757-57548776 None:intergenic 50.0%
! AGTGGTGGGGAGTAGAACAC+AGG - chr2_4:57548570-57548589 Msa0337510:CDS 55.0%
GTGGGGAGTAGAACACAGGT+TGG - chr2_4:57548574-57548593 Msa0337510:CDS 55.0%
AGGGAGGAAGAGGGATGTTG+TGG - chr2_4:57548633-57548652 Msa0337510:intron 55.0%
GAATGCGATTGAAGCGCGCA+TGG - chr2_4:57548795-57548814 Msa0337510:intron 55.0%
! AGTGGTGGGGAGTAGAACAC+AGG - chr2_4:57548570-57548589 Msa0337510:CDS 55.0%
GTGGGGAGTAGAACACAGGT+TGG - chr2_4:57548574-57548593 Msa0337510:CDS 55.0%
AGGGAGGAAGAGGGATGTTG+TGG - chr2_4:57548633-57548652 Msa0337510:intron 55.0%
GAATGCGATTGAAGCGCGCA+TGG - chr2_4:57548795-57548814 Msa0337510:intron 55.0%
GCGATTGAAGCGCGCATGGT+TGG - chr2_4:57548799-57548818 Msa0337510:intron 60.0%
GCGATTGAAGCGCGCATGGT+TGG - chr2_4:57548799-57548818 Msa0337510:intron 60.0%
! GAAGCGCGCATGGTTGGTGC+TGG - chr2_4:57548805-57548824 Msa0337510:intron 65.0%
! GAAGCGCGCATGGTTGGTGC+TGG - chr2_4:57548805-57548824 Msa0337510:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 57548529 57549352 57548529 ID=Msa0337510;Name=Msa0337510
chr2_4 mRNA 57548529 57549352 57548529 ID=Msa0337510-mRNA-1;Parent=Msa0337510;Name=Msa0337510-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0|1|0|0.5|2|0|121
chr2_4 exon 57548529 57548606 57548529 ID=Msa0337510-mRNA-1:exon:17533;Parent=Msa0337510-mRNA-1
chr2_4 exon 57549065 57549352 57549065 ID=Msa0337510-mRNA-1:exon:17532;Parent=Msa0337510-mRNA-1
chr2_4 CDS 57549065 57549352 57549065 ID=Msa0337510-mRNA-1:cds;Parent=Msa0337510-mRNA-1
chr2_4 CDS 57548529 57548606 57548529 ID=Msa0337510-mRNA-1:cds;Parent=Msa0337510-mRNA-1
Gene Sequence

>Msa0337510

AAAGAAAATGTGTCGTTGCGTGCTGAACAAAAGGATGGATCAGTGGTGGGGAGTAGAACACAGGTTGGTGAACTTGATGGAGGGAAGGCACGTTCGAAGAAACAAGGGAGGAAGAGGGATGTTGTGGATTTGAGGACCCTTTTGGTTCTGTGTGCACAAGCTGTGTCTGCTAATGACAATAGGACTGCTAATGAATTGTTGAAGCAGAATAGGAAGCATTCTTGTCCTTCAGGTGATGCATCACAGAGAATGGCTCATTACTTTGCGAATGCGATTGAAGCGCGCATGATTCAAGTCAACAGAAGTGACTATGACAATGTAATTGGAGCTTTGCCATATCTGAAAGTAAGCAGAAAAGCTATGTAA

Protein sequence

>Msa0337510

KENVSLRAEQKDGSVVGSRTQVGELDGGKARSKKQGRKRDVVDLRTLLVLCAQAVSANDNRTANELLKQNRKHSCPSGDASQRMAHYFANAIEARMIQVNRSDYDNVIGALPYLKVSRKAM*