AlfalfaGEDB | Msa0765280

M. sativa cultivar zhongmu-4 / Msa0765280

KEGG: K16282
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010498 GO:0010646 GO:0010647 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0023051 GO:0023056 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080134 GO:0140096 GO:1901419 GO:1901421 GO:1901564 GO:1901565 GO:1901575 GO:1905957 GO:1905959
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765280	AES96596.1	95.489	133	6	0	1	133	21	153	4.34e-90	268

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765280	sp\|Q9ZU51\|RHA2B_ARATH	29.126	103	71	2	1	102	20	121	1.87e-12	63.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765280	G7KB55	95.489	133	6	0	1	133	21	153	2.07e-90	268

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0724890	Msa0765280	0.870968	9.771295e-67	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765280	MtrunA17_Chr5g0416351	95.489	133	6	0	1	133	21	153	3.99e-94	268
Msa0765280	MtrunA17_Chr5g0416381	93.233	133	9	0	1	133	60	192	1.49e-93	268
Msa0765280	MtrunA17_Chr5g0416361	93.233	133	9	0	1	133	3	135	7.95e-92	261
Msa0765280	MtrunA17_Chr3g0104311	87.218	133	14	1	1	133	7	136	1.70e-83	240
Msa0765280	MtrunA17_Chr3g0104301	85.714	133	16	1	1	133	7	136	6.72e-83	239
Msa0765280	MtrunA17_Chr3g0104321	85.714	133	16	1	1	133	7	136	6.72e-83	239
Msa0765280	MtrunA17_Chr5g0427781	77.778	135	28	2	1	133	3	137	3.75e-74	217
Msa0765280	MtrunA17_Chr5g0427771	77.037	135	29	2	1	133	3	137	7.17e-73	214
Msa0765280	MtrunA17_Chr5g0425981	73.684	133	35	0	1	133	3	135	1.45e-72	213
Msa0765280	MtrunA17_Chr1g0171061	77.778	108	24	0	1	108	3	110	2.90e-62	187
Msa0765280	MtrunA17_Chr0c01g0489421	51.880	133	64	0	1	133	3	135	1.08e-46	147
Msa0765280	MtrunA17_Chr0c01g0489441	38.655	119	58	5	1	114	24	132	5.96e-19	77.4
Msa0765280	MtrunA17_Chr4g0039541	48.000	75	37	1	32	104	35	109	8.80e-18	73.9
Msa0765280	MtrunA17_Chr3g0124531	43.548	62	30	1	45	106	64	120	2.33e-13	63.2
Msa0765280	MtrunA17_Chr8g0369691	40.678	59	34	1	43	100	432	490	1.07e-12	63.5
Msa0765280	MtrunA17_Chr4g0065171	43.396	53	30	0	48	100	477	529	3.38e-12	62.4
Msa0765280	MtrunA17_Chr8g0387291	35.366	82	50	2	33	113	66	145	5.16e-12	59.7
Msa0765280	MtrunA17_Chr1g0148631	46.154	52	27	1	49	100	335	385	9.27e-12	60.8
Msa0765280	MtrunA17_Chr5g0408871	45.833	48	26	0	50	97	500	547	9.38e-12	60.8
Msa0765280	MtrunA17_Chr5g0397351	48.000	50	25	1	54	102	80	129	1.60e-11	57.8
Msa0765280	MtrunA17_Chr8g0382811	39.683	63	36	1	49	109	62	124	3.72e-11	57.4
Msa0765280	MtrunA17_Chr8g0364881	40.000	80	45	3	23	100	61	139	4.52e-11	58.9
Msa0765280	MtrunA17_Chr8g0348551	32.927	82	54	1	33	113	70	151	5.93e-11	57.0
Msa0765280	MtrunA17_Chr3g0123581	53.333	45	21	0	53	97	104	148	6.36e-11	56.6
Msa0765280	MtrunA17_Chr5g0413841	37.500	80	47	3	23	100	109	187	9.91e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765280	AT2G01150.1	29.126	103	71	2	1	102	20	121	1.90e-13	63.5
Msa0765280	AT2G01150.3	29.126	103	71	2	1	102	46	147	2.87e-13	63.5
Msa0765280	AT2G01150.2	29.126	103	71	2	1	102	69	170	5.05e-13	63.2
Msa0765280	AT2G04240.1	52.941	51	24	0	50	100	98	148	5.13e-13	62.8
Msa0765280	AT2G04240.2	52.941	51	24	0	50	100	98	148	5.13e-13	62.8
Msa0765280	AT3G61460.1	36.735	98	53	3	19	113	62	153	8.57e-13	62.4
Msa0765280	AT1G15100.1	43.636	55	31	0	54	108	85	139	5.86e-12	59.7
Msa0765280	AT1G23980.1	38.750	80	46	3	23	100	111	189	9.36e-12	61.2
Msa0765280	AT5G10650.1	37.500	56	34	1	51	106	471	525	3.24e-11	59.7
Msa0765280	AT5G10650.2	37.500	56	34	1	51	106	471	525	3.24e-11	59.7
Msa0765280	AT5G45290.1	44.898	49	27	0	49	97	484	532	8.83e-11	58.5
Msa0765280	AT1G53820.1	44.286	70	35	3	50	117	115	182	9.62e-11	58.2

Find 23 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAATATTGTTTAGATAAA+TGG	0.100921	5_2:-25968042	Msa0765280:CDS
AACTCGGTGATGGCCCTTTC+TGG	0.154175	5_2:+25967971	None:intergenic
TAGTTTACACTCATATAAAA+TGG	0.187893	5_2:-25968258	Msa0765280:CDS
TCATACATTTCTCCTATTAC+TGG	0.278088	5_2:+25968181	None:intergenic
GAAAGGGCCATCACCGAGTT+TGG	0.327950	5_2:-25967968	Msa0765280:CDS
AGAATCATGAAGCTTGTAGA+AGG	0.385315	5_2:+25968210	None:intergenic
AACATCTTCCTTTGAGCCAT+GGG	0.423046	5_2:+25968138	None:intergenic
CAACATCTTCCTTTGAGCCA+TGG	0.448119	5_2:+25968137	None:intergenic
AACAATATTTGTGATACATA+TGG	0.455611	5_2:+25968053	None:intergenic
TCTCAATGATTCTCTACAAA+GGG	0.470759	5_2:+25967994	None:intergenic
AGTTTACACTCATATAAAAT+GGG	0.485230	5_2:-25968257	Msa0765280:CDS
TGTCTCTGCAAAATAGAAGA+AGG	0.553274	5_2:-25968103	Msa0765280:CDS
TTCAGCTCCAAACTCGGTGA+TGG	0.557942	5_2:+25967961	None:intergenic
GTCTCAATGATTCTCTACAA+AGG	0.562350	5_2:+25967993	None:intergenic
AGAATCATTGAGACCAGAAA+GGG	0.573118	5_2:-25967984	Msa0765280:CDS
AAGAATTTCAGCTCCAAACT+CGG	0.579655	5_2:+25967955	None:intergenic
GAGAATCATTGAGACCAGAA+AGG	0.589828	5_2:-25967985	Msa0765280:CDS
TGAACACACCCATGGCTCAA+AGG	0.591217	5_2:-25968146	Msa0765280:CDS
TGCAATTATGAACACACCCA+TGG	0.592390	5_2:-25968154	Msa0765280:CDS
GAATCATGAAGCTTGTAGAA+GGG	0.606509	5_2:+25968211	None:intergenic
CTCTACAAAGGGGACAAGTG+TGG	0.650261	5_2:+25968005	None:intergenic
CATCACGATCTCGATCACTA+CGG	0.660210	5_2:+25967912	None:intergenic
CTCAATGATTCTCTACAAAG+GGG	0.693684	5_2:+25967995	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAAATATTGTTTAGATAAA+TGG	-	chr5_2:25968127-25968146	Msa0765280:CDS	15.0%
!!	TAGTTTACACTCATATAAAA+TGG	-	chr5_2:25967911-25967930	Msa0765280:CDS	20.0%
!!	AGTTTACACTCATATAAAAT+GGG	-	chr5_2:25967912-25967931	Msa0765280:CDS	20.0%
!!	AACAATATTTGTGATACATA+TGG	+	chr5_2:25968119-25968138	None:intergenic	20.0%
!!	GATTCTAATTTTCCAGTAAT+AGG	-	chr5_2:25967976-25967995	Msa0765280:CDS	25.0%
	TCATACATTTCTCCTATTAC+TGG	+	chr5_2:25967991-25968010	None:intergenic	30.0%
	TCTCAATGATTCTCTACAAA+GGG	+	chr5_2:25968178-25968197	None:intergenic	30.0%
	GAATCATGAAGCTTGTAGAA+GGG	+	chr5_2:25967961-25967980	None:intergenic	35.0%
	AGAATCATGAAGCTTGTAGA+AGG	+	chr5_2:25967962-25967981	None:intergenic	35.0%
	TGTCTCTGCAAAATAGAAGA+AGG	-	chr5_2:25968066-25968085	Msa0765280:CDS	35.0%
	CTCAATGATTCTCTACAAAG+GGG	+	chr5_2:25968177-25968196	None:intergenic	35.0%
	GTCTCAATGATTCTCTACAA+AGG	+	chr5_2:25968179-25968198	None:intergenic	35.0%
	AGAATCATTGAGACCAGAAA+GGG	-	chr5_2:25968185-25968204	Msa0765280:CDS	35.0%
	AAGAATTTCAGCTCCAAACT+CGG	+	chr5_2:25968217-25968236	None:intergenic	35.0%
!	ATCGAGATCGTGATGATTTT+TGG	-	chr5_2:25968265-25968284	Msa0765280:CDS	35.0%
	TGCAATTATGAACACACCCA+TGG	-	chr5_2:25968015-25968034	Msa0765280:CDS	40.0%
	AACATCTTCCTTTGAGCCAT+GGG	+	chr5_2:25968034-25968053	None:intergenic	40.0%
	GAGAATCATTGAGACCAGAA+AGG	-	chr5_2:25968184-25968203	Msa0765280:CDS	40.0%
	CAACATCTTCCTTTGAGCCA+TGG	+	chr5_2:25968035-25968054	None:intergenic	45.0%
	CATCACGATCTCGATCACTA+CGG	+	chr5_2:25968260-25968279	None:intergenic	45.0%
	TGAACACACCCATGGCTCAA+AGG	-	chr5_2:25968023-25968042	Msa0765280:CDS	50.0%
	CTCTACAAAGGGGACAAGTG+TGG	+	chr5_2:25968167-25968186	None:intergenic	50.0%
!	TTCAGCTCCAAACTCGGTGA+TGG	+	chr5_2:25968211-25968230	None:intergenic	50.0%
!	AACTCGGTGATGGCCCTTTC+TGG	+	chr5_2:25968201-25968220	None:intergenic	55.0%
	GAAAGGGCCATCACCGAGTT+TGG	-	chr5_2:25968201-25968220	Msa0765280:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	25967895	25968296	25967895	ID=Msa0765280;Name=Msa0765280
chr5_2	mRNA	25967895	25968296	25967895	ID=Msa0765280-mRNA-1;Parent=Msa0765280;Name=Msa0765280-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|133
chr5_2	exon	25967895	25968296	25967895	ID=Msa0765280-mRNA-1:exon:10392;Parent=Msa0765280-mRNA-1
chr5_2	CDS	25967895	25968296	25967895	ID=Msa0765280-mRNA-1:cds;Parent=Msa0765280-mRNA-1

Gene Sequence

>Msa0765280

ATGAAGTACTTAACTCTAGTTTACACTCATATAAAATGGGTATTAGATATTCTTACATATTATCCCTTCTACAAGCTTCATGATTCTAATTTTCCAGTAATAGGAGAAATGTATGATATATGCAATTATGAACACACCCATGGCTCAAAGGAAGATGTTGAATGTGCTGTGTGTCTCTGCAAAATAGAAGAAGGAGATGAGATTAGTGTATTGAGATGTGACCATATGTATCACAAATATTGTTTAGATAAATGGATTAGTTTCAAGAACCACACTTGTCCCCTTTGTAGAGAATCATTGAGACCAGAAAGGGCCATCACCGAGTTTGGAGCTGAAATTCTTTCATTTAATTTTTGTGTCATCCGTAGTGATCGAGATCGTGATGATTTTTGGCTACGTTGA

Protein sequence

>Msa0765280

MKYLTLVYTHIKWVLDILTYYPFYKLHDSNFPVIGEMYDICNYEHTHGSKEDVECAVCLCKIEEGDEISVLRCDHMYHKYCLDKWISFKNHTCPLCRESLRPERAITEFGAEILSFNFCVIRSDRDRDDFWLR*