AlfalfaGEDB | Msa0724890

M. sativa cultivar zhongmu-4 / Msa0724890

KEGG: K16282
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010498 GO:0010646 GO:0010647 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0023051 GO:0023056 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080134 GO:0140096 GO:1901419 GO:1901421 GO:1901564 GO:1901565 GO:1901575 GO:1905957 GO:1905959
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0724890	AES96596.1	94.737	133	7	0	1	133	21	153	6.24e-89	265

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0724890	sp\|Q9XF92\|BRH1_ARATH	37.755	98	52	3	19	113	62	153	1.30e-12	64.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0724890	G7KB55	94.737	133	7	0	1	133	21	153	2.98e-89	265

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0724890	Msa0765280	0.870968	9.771295e-67	-8.615850e-47
Msa0724890	Msa0787720	0.801872	7.335098e-49	-8.615850e-47
Msa0724890	Msa0950620	0.808130	3.603151e-50	-8.615850e-47
Msa0223100	Msa0724890	0.803959	2.717096e-49	-8.615850e-47
Msa0223380	Msa0724890	0.803574	3.266960e-49	-8.615850e-47
Msa0505060	Msa0724890	0.800479	1.413891e-48	-8.615850e-47
Msa0530380	Msa0724890	0.809335	1.990513e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0724890	MtrunA17_Chr5g0416351	94.737	133	7	0	1	133	21	153	5.74e-93	265
Msa0724890	MtrunA17_Chr5g0416381	92.481	133	10	0	1	133	60	192	2.09e-92	265
Msa0724890	MtrunA17_Chr5g0416361	92.481	133	10	0	1	133	3	135	7.88e-91	259
Msa0724890	MtrunA17_Chr3g0104311	86.466	133	15	1	1	133	7	136	3.84e-82	237
Msa0724890	MtrunA17_Chr3g0104301	84.962	133	17	1	1	133	7	136	1.40e-81	236
Msa0724890	MtrunA17_Chr3g0104321	84.962	133	17	1	1	133	7	136	1.40e-81	236
Msa0724890	MtrunA17_Chr5g0427781	77.037	135	29	2	1	133	3	137	6.15e-73	214
Msa0724890	MtrunA17_Chr5g0427771	76.296	135	30	2	1	133	3	137	1.10e-71	211
Msa0724890	MtrunA17_Chr5g0425981	72.932	133	36	0	1	133	3	135	2.48e-71	209
Msa0724890	MtrunA17_Chr1g0171061	76.852	108	25	0	1	108	3	110	6.73e-61	183
Msa0724890	MtrunA17_Chr0c01g0489421	51.128	133	65	0	1	133	3	135	1.75e-45	144
Msa0724890	MtrunA17_Chr0c01g0489441	38.655	119	58	5	1	114	24	132	4.75e-19	77.4
Msa0724890	MtrunA17_Chr4g0039541	48.000	75	37	1	32	104	35	109	6.10e-18	74.3
Msa0724890	MtrunA17_Chr5g0397351	50.000	50	24	1	54	102	80	129	1.50e-12	60.5
Msa0724890	MtrunA17_Chr3g0124531	41.935	62	31	1	45	106	64	120	4.54e-12	59.7
Msa0724890	MtrunA17_Chr8g0387291	43.333	60	33	1	55	113	86	145	6.44e-12	59.3
Msa0724890	MtrunA17_Chr8g0348551	34.146	82	53	1	33	113	70	151	7.89e-12	59.3
Msa0724890	MtrunA17_Chr1g0148631	46.154	52	27	1	49	100	335	385	8.33e-12	61.2
Msa0724890	MtrunA17_Chr8g0364881	40.000	80	45	3	23	100	61	139	1.09e-11	60.5
Msa0724890	MtrunA17_Chr3g0120681	50.980	51	24	1	54	103	98	148	1.47e-11	58.2
Msa0724890	MtrunA17_Chr8g0369691	41.071	56	32	1	43	97	432	487	1.70e-11	60.1
Msa0724890	MtrunA17_Chr5g0413841	37.500	80	47	3	23	100	109	187	2.83e-11	59.7
Msa0724890	MtrunA17_Chr4g0065171	41.509	53	31	0	48	100	477	529	4.52e-11	58.9
Msa0724890	MtrunA17_Chr4g0059281	36.250	80	48	3	23	100	99	177	4.53e-11	58.9
Msa0724890	MtrunA17_Chr3g0123581	52.174	46	22	0	53	98	104	149	6.43e-11	56.6
Msa0724890	MtrunA17_Chr3g0138951	40.385	52	30	1	49	100	482	532	8.79e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0724890	AT3G61460.1	37.755	98	52	3	19	113	62	153	1.33e-13	64.3
Msa0724890	AT2G04240.1	52.941	51	24	0	50	100	98	148	1.73e-13	63.9
Msa0724890	AT2G04240.2	52.941	51	24	0	50	100	98	148	1.73e-13	63.9
Msa0724890	AT2G01150.1	28.155	103	72	2	1	102	20	121	2.97e-12	60.5
Msa0724890	AT1G23980.1	38.750	80	46	3	23	100	111	189	3.75e-12	62.4
Msa0724890	AT2G01150.3	28.155	103	72	2	1	102	46	147	4.74e-12	60.5
Msa0724890	AT2G01150.2	28.155	103	72	2	1	102	69	170	7.79e-12	60.1
Msa0724890	AT1G63840.1	33.663	101	64	3	22	120	57	156	3.43e-11	57.8
Msa0724890	AT1G15100.1	41.818	55	32	0	54	108	85	139	9.03e-11	56.6

Find 22 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAATATTGTTTAGATAAA+TGG	0.100921	5_1:+24013411	Msa0724890:CDS
AACTCGGTGATGGCCCTTTC+TGG	0.154175	5_1:-24013482	None:intergenic
TAGTTTACACTCATATAAAA+TGG	0.187893	5_1:+24013195	Msa0724890:CDS
TCATACATTTCTCCTATTAC+TGG	0.278088	5_1:-24013272	None:intergenic
GAAAGGGCCATCACCGAGTT+TGG	0.327950	5_1:+24013485	Msa0724890:CDS
AGAATCATGAAGCTTGTAGA+AGG	0.385315	5_1:-24013243	None:intergenic
AACATCTTCCTTTGAGCCAT+GGG	0.423046	5_1:-24013315	None:intergenic
CAACATCTTCCTTTGAGCCA+TGG	0.448119	5_1:-24013316	None:intergenic
AACAATATTTGTGATACATA+TGG	0.455611	5_1:-24013400	None:intergenic
TCTCAATGATTCTCTACAAA+GGG	0.470759	5_1:-24013459	None:intergenic
AGTTTACACTCATATAAAAT+GGG	0.485230	5_1:+24013196	Msa0724890:CDS
TTCAGCTCCAAACTCGGTGA+TGG	0.557942	5_1:-24013492	None:intergenic
GTCTCAATGATTCTCTACAA+AGG	0.562350	5_1:-24013460	None:intergenic
AGAATCATTGAGACCAGAAA+GGG	0.573118	5_1:+24013469	Msa0724890:CDS
AAGAATTTCAGCTCCAAACT+CGG	0.579655	5_1:-24013498	None:intergenic
GAGAATCATTGAGACCAGAA+AGG	0.589828	5_1:+24013468	Msa0724890:CDS
TGAACACACCCATGGCTCAA+AGG	0.591217	5_1:+24013307	Msa0724890:CDS
TGCAATTATGAACACACCCA+TGG	0.592390	5_1:+24013299	Msa0724890:CDS
GAATCATGAAGCTTGTAGAA+GGG	0.606509	5_1:-24013242	None:intergenic
CTCTACAAAGGGGACAAGTG+TGG	0.650261	5_1:-24013448	None:intergenic
CATCACGATCTCGATCACTA+CGG	0.660210	5_1:-24013541	None:intergenic
CTCAATGATTCTCTACAAAG+GGG	0.693684	5_1:-24013458	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAAATATTGTTTAGATAAA+TGG	+	chr5_1:24013411-24013430	Msa0724890:CDS	15.0%
!!	TAGTTTACACTCATATAAAA+TGG	+	chr5_1:24013195-24013214	Msa0724890:CDS	20.0%
!!	AGTTTACACTCATATAAAAT+GGG	+	chr5_1:24013196-24013215	Msa0724890:CDS	20.0%
!!	AACAATATTTGTGATACATA+TGG	-	chr5_1:24013403-24013422	None:intergenic	20.0%
!!	GATTCTAATTTTCCAGTAAT+AGG	+	chr5_1:24013260-24013279	Msa0724890:CDS	25.0%
	TCATACATTTCTCCTATTAC+TGG	-	chr5_1:24013275-24013294	None:intergenic	30.0%
	TCTCAATGATTCTCTACAAA+GGG	-	chr5_1:24013462-24013481	None:intergenic	30.0%
	GAATCATGAAGCTTGTAGAA+GGG	-	chr5_1:24013245-24013264	None:intergenic	35.0%
	AGAATCATGAAGCTTGTAGA+AGG	-	chr5_1:24013246-24013265	None:intergenic	35.0%
	CTCAATGATTCTCTACAAAG+GGG	-	chr5_1:24013461-24013480	None:intergenic	35.0%
	GTCTCAATGATTCTCTACAA+AGG	-	chr5_1:24013463-24013482	None:intergenic	35.0%
	AGAATCATTGAGACCAGAAA+GGG	+	chr5_1:24013469-24013488	Msa0724890:CDS	35.0%
	AAGAATTTCAGCTCCAAACT+CGG	-	chr5_1:24013501-24013520	None:intergenic	35.0%
!	ATCGAGATCGTGATGATTTT+TGG	+	chr5_1:24013549-24013568	Msa0724890:CDS	35.0%
	TGCAATTATGAACACACCCA+TGG	+	chr5_1:24013299-24013318	Msa0724890:CDS	40.0%
	AACATCTTCCTTTGAGCCAT+GGG	-	chr5_1:24013318-24013337	None:intergenic	40.0%
	GAGAATCATTGAGACCAGAA+AGG	+	chr5_1:24013468-24013487	Msa0724890:CDS	40.0%
	CAACATCTTCCTTTGAGCCA+TGG	-	chr5_1:24013319-24013338	None:intergenic	45.0%
	CATCACGATCTCGATCACTA+CGG	-	chr5_1:24013544-24013563	None:intergenic	45.0%
	TGAACACACCCATGGCTCAA+AGG	+	chr5_1:24013307-24013326	Msa0724890:CDS	50.0%
	CTCTACAAAGGGGACAAGTG+TGG	-	chr5_1:24013451-24013470	None:intergenic	50.0%
!	TTCAGCTCCAAACTCGGTGA+TGG	-	chr5_1:24013495-24013514	None:intergenic	50.0%
!	AACTCGGTGATGGCCCTTTC+TGG	-	chr5_1:24013485-24013504	None:intergenic	55.0%
	GAAAGGGCCATCACCGAGTT+TGG	+	chr5_1:24013485-24013504	Msa0724890:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	24013179	24013580	24013179	ID=Msa0724890;Name=Msa0724890
chr5_1	mRNA	24013179	24013580	24013179	ID=Msa0724890-mRNA-1;Parent=Msa0724890;Name=Msa0724890-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|133
chr5_1	exon	24013179	24013580	24013179	ID=Msa0724890-mRNA-1:exon:8844;Parent=Msa0724890-mRNA-1
chr5_1	CDS	24013179	24013580	24013179	ID=Msa0724890-mRNA-1:cds;Parent=Msa0724890-mRNA-1

Gene Sequence

>Msa0724890

ATGAAGTACTTAACTCTAGTTTACACTCATATAAAATGGGTATTAGATATTCTTACATATTATCCCTTCTACAAGCTTCATGATTCTAATTTTCCAGTAATAGGAGAAATGTATGATATATGCAATTATGAACACACCCATGGCTCAAAGGAAGATGTTGAATGTGCTGTGTGTCTCTGCAAAATAGAAGAAGAAGATGAGATTAGTGTATTGAGATGTGACCATATGTATCACAAATATTGTTTAGATAAATGGATTAGTTTCAAGAACCACACTTGTCCCCTTTGTAGAGAATCATTGAGACCAGAAAGGGCCATCACCGAGTTTGGAGCTGAAATTCTTTCATTTAATTTTTGTGTCATCCGTAGTGATCGAGATCGTGATGATTTTTGGCTACGTTGA

Protein sequence

>Msa0724890

MKYLTLVYTHIKWVLDILTYYPFYKLHDSNFPVIGEMYDICNYEHTHGSKEDVECAVCLCKIEEEDEISVLRCDHMYHKYCLDKWISFKNHTCPLCRESLRPERAITEFGAEILSFNFCVIRSDRDRDDFWLR*