AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0768830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0768830 MtrunA17_Chr5g0419811 83.957 187 19 2 34 209 1 187 2.09e-108 308
Msa0768830 MtrunA17_Chr3g0109391 66.667 123 30 2 60 182 587 698 1.66e-42 152
Msa0768830 MtrunA17_Chr5g0433301 51.639 122 46 2 61 182 581 689 3.94e-29 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCTTACCACATTGTTTC+TGG 0.133950 5_2:+35613625 Msa0768830:three_prime_UTR
ACAATGCAATTAGGGGAATT+TGG 0.182664 5_2:+35613364 Msa0768830:CDS
ATGTCGCATGCGCAATATTC+TGG 0.202860 5_2:+35613166 Msa0768830:CDS
CACCTGAGAAAATATGATTT+TGG 0.206900 5_2:-35613231 None:intergenic
ACCAATTCAAATATCATCTT+TGG 0.219702 5_2:+35612919 Msa0768830:CDS
CCCAACTTTCTCTTATAAAT+AGG 0.235957 5_2:-35613676 Msa0768840:intron
TTGGAAGGAGTATTACATTT+TGG 0.238603 5_2:-35613652 Msa0768840:CDS
GGTGCTTCGACAATGCAATT+AGG 0.245347 5_2:+35613355 Msa0768830:CDS
GTGCTTCGACAATGCAATTA+GGG 0.285038 5_2:+35613356 Msa0768830:CDS
CAAGCGGGAGAAATACATTA+AGG 0.307728 5_2:+35613594 Msa0768830:three_prime_UTR
CCACTACTTGGTAAACTTGA+TGG 0.323390 5_2:-35613058 None:intergenic
CCGGGTTACACCACTTCATT+AGG 0.330990 5_2:+35613325 Msa0768830:CDS
TTAACTAAAGTTGATGAATC+TGG 0.331573 5_2:-35613735 Msa0768840:intron
GTTGGAAATTCATTTGATAT+TGG 0.350671 5_2:+35612974 Msa0768830:CDS
TCAACAAAAGTTGATGTATC+TGG 0.356195 5_2:+35613699 Msa0768830:three_prime_UTR
GATCATTCTTGAATAGCATT+TGG 0.373233 5_2:+35613526 Msa0768830:intron
TCATCGTTGCATTGCACTAA+AGG 0.394418 5_2:+35613016 Msa0768830:CDS
ACCTGAAGATATTCAAAAGA+TGG 0.405470 5_2:+35613566 Msa0768830:CDS
TCATCTTTGGATTCAACAAT+GGG 0.406650 5_2:+35612932 Msa0768830:CDS
GATTCAACAATGGGGAGTAT+TGG 0.411860 5_2:+35612941 Msa0768830:CDS
TCCAAAGATGATATTTGAAT+TGG 0.413568 5_2:-35612920 None:intergenic
AACAATTACCTCAACAATCA+TGG 0.416923 5_2:+35613098 Msa0768830:CDS
CCTATTTATAAGAGAAAGTT+GGG 0.422085 5_2:+35613676 Msa0768830:three_prime_UTR
AAAGATGATATTTGAATTGG+TGG 0.425517 5_2:-35612917 None:intergenic
TCATCTTTATTATTTATGTC+AGG 0.427766 5_2:+35613770 Msa0768830:three_prime_UTR
GCAATATTCTGGACAAGATT+CGG 0.442351 5_2:+35613177 Msa0768830:CDS
TCCTATTTATAAGAGAAAGT+TGG 0.442773 5_2:+35613675 Msa0768830:three_prime_UTR
GATATTTGAATTGGTGGAAC+TGG 0.454943 5_2:-35612911 None:intergenic
TCTACTCCCCACCACTGTCC+CGG 0.468646 5_2:+35613306 Msa0768830:CDS
CTCTTATAAATAGGATTGGA+AGG 0.470484 5_2:-35613667 Msa0768840:intron
CTTTCTCTTATAAATAGGAT+TGG 0.470767 5_2:-35613671 Msa0768840:intron
TTGCATTGCACTAAAGGAAA+AGG 0.472909 5_2:+35613022 Msa0768830:CDS
GGACATCTAAATCAAAATGT+GGG 0.484157 5_2:-35613210 None:intergenic
TGCTCAAGTGAGGTAGAAGT+TGG 0.486956 5_2:-35613880 Msa0768840:intron
GTGTAACCCGGGACAGTGGT+GGG 0.487177 5_2:-35613313 None:intergenic
GGGGAGTATTGGACAAGGAT+TGG 0.490348 5_2:+35612952 Msa0768830:CDS
ATCAACAACGGACCACTACT+TGG 0.491789 5_2:-35613070 None:intergenic
ATCATCTTTGGATTCAACAA+TGG 0.498569 5_2:+35612931 Msa0768830:CDS
TAGTGCAATGCAACGATGAA+TGG 0.498785 5_2:-35613012 None:intergenic
TGGACATCTAAATCAAAATG+TGG 0.502102 5_2:-35613211 None:intergenic
TCAAAAGATGGGATAACAAG+CGG 0.507610 5_2:+35613578 Msa0768830:exon
AAGCGGGAGAAATACATTAA+GGG 0.512222 5_2:+35613595 Msa0768830:three_prime_UTR
GGTGTAACCCGGGACAGTGG+TGG 0.512415 5_2:-35613314 None:intergenic
AGTATTGGACAAGGATTGGT+TGG 0.518348 5_2:+35612956 Msa0768830:CDS
GAGAAATACATTAAGGGCAT+TGG 0.518833 5_2:+35613601 Msa0768830:three_prime_UTR
ACCACTTCATTAGGTGAGAC+CGG 0.518859 5_2:+35613334 Msa0768830:CDS
CCTGAAGATATTCAAAAGAT+GGG 0.518915 5_2:+35613567 Msa0768830:CDS
CCTAATGAAGTGGTGTAACC+CGG 0.520984 5_2:-35613325 None:intergenic
CTACTCCCCACCACTGTCCC+GGG 0.522171 5_2:+35613307 Msa0768830:CDS
ATTAGGGGAATTTGGATTCG+AGG 0.533461 5_2:+35613372 Msa0768830:CDS
TGTAACCCGGGACAGTGGTG+GGG 0.544537 5_2:-35613312 None:intergenic
AGTATGCAGGTCGATACCTT+TGG 0.549314 5_2:+35613136 Msa0768830:CDS
ATTTGAATTGGTGGAACTGG+TGG 0.550148 5_2:-35612908 None:intergenic
CAAAAGATGGGATAACAAGC+GGG 0.554197 5_2:+35613579 Msa0768830:exon
TAGAAGATCAGATGAATCGA+TGG 0.554577 5_2:-35613287 None:intergenic
ACCTGCATACTTTGATACCT+CGG 0.565962 5_2:-35613124 None:intergenic
TTAGGGGAATTTGGATTCGA+GGG 0.567383 5_2:+35613373 Msa0768830:CDS
AGATCAGATGAATCGATGGT+GGG 0.567478 5_2:-35613283 None:intergenic
TAATTGCATTGTCGAAGCAC+CGG 0.570679 5_2:-35613353 None:intergenic
TCCGAGGTATCAAAGTATGC+AGG 0.573808 5_2:+35613123 Msa0768830:CDS
ATGCGACATTGCGTTGCCAA+AGG 0.575172 5_2:-35613152 None:intergenic
AGCATTATATCTGAATACAC+TGG 0.584252 5_2:+35612866 Msa0768830:CDS
GACATCTAAATCAAAATGTG+GGG 0.585558 5_2:-35613209 None:intergenic
ACCGGTCTCACCTAATGAAG+TGG 0.597496 5_2:-35613335 None:intergenic
AAGATCAGATGAATCGATGG+TGG 0.602370 5_2:-35613284 None:intergenic
GAAACTGGTACTTACTCCCA+CGG 0.602394 5_2:-35613406 None:intergenic
GATCAGATGAATCGATGGTG+GGG 0.608527 5_2:-35613282 None:intergenic
CCATCAAGTTTACCAAGTAG+TGG 0.609404 5_2:+35613058 Msa0768830:CDS
CTCGGAGTCCATGATTGTTG+AGG 0.616580 5_2:-35613106 None:intergenic
AATTGTTCATTCATCAACAA+CGG 0.631258 5_2:-35613082 None:intergenic
CTAATGAAGTGGTGTAACCC+GGG 0.645481 5_2:-35613324 None:intergenic
TTGGTTGTCCAGAAACAATG+TGG 0.649003 5_2:-35613633 Msa0768840:CDS
TGCTTCGACAATGCAATTAG+GGG 0.669628 5_2:+35613357 Msa0768830:CDS
CTCAACAATCATGGACTCCG+AGG 0.676275 5_2:+35613107 Msa0768830:CDS
ACATCTAAATCAAAATGTGG+GGG 0.693255 5_2:-35613208 None:intergenic
ACAATGGGGAGTATTGGACA+AGG 0.710979 5_2:+35612947 Msa0768830:CDS
AGTGGTGTAACCCGGGACAG+TGG 0.726941 5_2:-35613317 None:intergenic
CATCTTTGGATTCAACAATG+GGG 0.731611 5_2:+35612933 Msa0768830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATCTTTATTATTTATGTC+AGG + chr5_2:35613770-35613789 Msa0768830:three_prime_UTR 20.0%
!! TCATCTTTATTATTTATGTC+AGG + chr5_2:35613770-35613789 Msa0768830:three_prime_UTR 20.0%
! AAAGATGATATTTGAATTGG+TGG - chr5_2:35612920-35612939 None:intergenic 25.0%
! ACCAATTCAAATATCATCTT+TGG + chr5_2:35612919-35612938 Msa0768830:CDS 25.0%
! TCCAAAGATGATATTTGAAT+TGG - chr5_2:35612923-35612942 None:intergenic 25.0%
! GTTGGAAATTCATTTGATAT+TGG + chr5_2:35612974-35612993 Msa0768830:CDS 25.0%
! AATTGTTCATTCATCAACAA+CGG - chr5_2:35613085-35613104 None:intergenic 25.0%
! CTTTCTCTTATAAATAGGAT+TGG - chr5_2:35613674-35613693 Msa0768840:intron 25.0%
! TCCTATTTATAAGAGAAAGT+TGG + chr5_2:35613675-35613694 Msa0768830:three_prime_UTR 25.0%
! CCTATTTATAAGAGAAAGTT+GGG + chr5_2:35613676-35613695 Msa0768830:three_prime_UTR 25.0%
!!! TTAACTAAAGTTGATGAATC+TGG - chr5_2:35613738-35613757 Msa0768840:intron 25.0%
! AAAGATGATATTTGAATTGG+TGG - chr5_2:35612920-35612939 None:intergenic 25.0%
! ACCAATTCAAATATCATCTT+TGG + chr5_2:35612919-35612938 Msa0768830:CDS 25.0%
! TCCAAAGATGATATTTGAAT+TGG - chr5_2:35612923-35612942 None:intergenic 25.0%
! GTTGGAAATTCATTTGATAT+TGG + chr5_2:35612974-35612993 Msa0768830:CDS 25.0%
! AATTGTTCATTCATCAACAA+CGG - chr5_2:35613085-35613104 None:intergenic 25.0%
! CTTTCTCTTATAAATAGGAT+TGG - chr5_2:35613674-35613693 Msa0768840:intron 25.0%
! TCCTATTTATAAGAGAAAGT+TGG + chr5_2:35613675-35613694 Msa0768830:three_prime_UTR 25.0%
! CCTATTTATAAGAGAAAGTT+GGG + chr5_2:35613676-35613695 Msa0768830:three_prime_UTR 25.0%
!!! TTAACTAAAGTTGATGAATC+TGG - chr5_2:35613738-35613757 Msa0768840:intron 25.0%
AGCATTATATCTGAATACAC+TGG + chr5_2:35612866-35612885 Msa0768830:CDS 30.0%
ATCATCTTTGGATTCAACAA+TGG + chr5_2:35612931-35612950 Msa0768830:CDS 30.0%
TCATCTTTGGATTCAACAAT+GGG + chr5_2:35612932-35612951 Msa0768830:CDS 30.0%
AACAATTACCTCAACAATCA+TGG + chr5_2:35613098-35613117 Msa0768830:CDS 30.0%
ACATCTAAATCAAAATGTGG+GGG - chr5_2:35613211-35613230 None:intergenic 30.0%
GACATCTAAATCAAAATGTG+GGG - chr5_2:35613212-35613231 None:intergenic 30.0%
GGACATCTAAATCAAAATGT+GGG - chr5_2:35613213-35613232 None:intergenic 30.0%
TGGACATCTAAATCAAAATG+TGG - chr5_2:35613214-35613233 None:intergenic 30.0%
! GTCCAAAATCATATTTTCTC+AGG + chr5_2:35613229-35613248 Msa0768830:CDS 30.0%
! CACCTGAGAAAATATGATTT+TGG - chr5_2:35613234-35613253 None:intergenic 30.0%
!! CTTGATGATGTAATTGAAAC+TGG - chr5_2:35613424-35613443 None:intergenic 30.0%
GATCATTCTTGAATAGCATT+TGG + chr5_2:35613526-35613545 Msa0768830:intron 30.0%
ACCTGAAGATATTCAAAAGA+TGG + chr5_2:35613566-35613585 Msa0768830:CDS 30.0%
CCTGAAGATATTCAAAAGAT+GGG + chr5_2:35613567-35613586 Msa0768830:CDS 30.0%
!!! TTGGAAGGAGTATTACATTT+TGG - chr5_2:35613655-35613674 Msa0768840:CDS 30.0%
CTCTTATAAATAGGATTGGA+AGG - chr5_2:35613670-35613689 Msa0768840:intron 30.0%
CCCAACTTTCTCTTATAAAT+AGG - chr5_2:35613679-35613698 Msa0768840:intron 30.0%
!! TCAACAAAAGTTGATGTATC+TGG + chr5_2:35613699-35613718 Msa0768830:three_prime_UTR 30.0%
AGCATTATATCTGAATACAC+TGG + chr5_2:35612866-35612885 Msa0768830:CDS 30.0%
ATCATCTTTGGATTCAACAA+TGG + chr5_2:35612931-35612950 Msa0768830:CDS 30.0%
TCATCTTTGGATTCAACAAT+GGG + chr5_2:35612932-35612951 Msa0768830:CDS 30.0%
AACAATTACCTCAACAATCA+TGG + chr5_2:35613098-35613117 Msa0768830:CDS 30.0%
ACATCTAAATCAAAATGTGG+GGG - chr5_2:35613211-35613230 None:intergenic 30.0%
GACATCTAAATCAAAATGTG+GGG - chr5_2:35613212-35613231 None:intergenic 30.0%
GGACATCTAAATCAAAATGT+GGG - chr5_2:35613213-35613232 None:intergenic 30.0%
TGGACATCTAAATCAAAATG+TGG - chr5_2:35613214-35613233 None:intergenic 30.0%
! GTCCAAAATCATATTTTCTC+AGG + chr5_2:35613229-35613248 Msa0768830:CDS 30.0%
! CACCTGAGAAAATATGATTT+TGG - chr5_2:35613234-35613253 None:intergenic 30.0%
!! CTTGATGATGTAATTGAAAC+TGG - chr5_2:35613424-35613443 None:intergenic 30.0%
GATCATTCTTGAATAGCATT+TGG + chr5_2:35613526-35613545 Msa0768830:intron 30.0%
ACCTGAAGATATTCAAAAGA+TGG + chr5_2:35613566-35613585 Msa0768830:CDS 30.0%
CCTGAAGATATTCAAAAGAT+GGG + chr5_2:35613567-35613586 Msa0768830:CDS 30.0%
!!! TTGGAAGGAGTATTACATTT+TGG - chr5_2:35613655-35613674 Msa0768840:CDS 30.0%
CTCTTATAAATAGGATTGGA+AGG - chr5_2:35613670-35613689 Msa0768840:intron 30.0%
CCCAACTTTCTCTTATAAAT+AGG - chr5_2:35613679-35613698 Msa0768840:intron 30.0%
!! TCAACAAAAGTTGATGTATC+TGG + chr5_2:35613699-35613718 Msa0768830:three_prime_UTR 30.0%
! TACACTGGAAGATCAGTTTT+AGG + chr5_2:35612881-35612900 Msa0768830:CDS 35.0%
! ACACTGGAAGATCAGTTTTA+GGG + chr5_2:35612882-35612901 Msa0768830:CDS 35.0%
GATATTTGAATTGGTGGAAC+TGG - chr5_2:35612914-35612933 None:intergenic 35.0%
CATCTTTGGATTCAACAATG+GGG + chr5_2:35612933-35612952 Msa0768830:CDS 35.0%
TTGCATTGCACTAAAGGAAA+AGG + chr5_2:35613022-35613041 Msa0768830:CDS 35.0%
GCAATATTCTGGACAAGATT+CGG + chr5_2:35613177-35613196 Msa0768830:CDS 35.0%
TAGAAGATCAGATGAATCGA+TGG - chr5_2:35613290-35613309 None:intergenic 35.0%
ACAATGCAATTAGGGGAATT+TGG + chr5_2:35613364-35613383 Msa0768830:CDS 35.0%
! CCCATCTTTTGAATATCTTC+AGG - chr5_2:35613570-35613589 None:intergenic 35.0%
TCAAAAGATGGGATAACAAG+CGG + chr5_2:35613578-35613597 Msa0768830:exon 35.0%
AAGCGGGAGAAATACATTAA+GGG + chr5_2:35613595-35613614 Msa0768830:three_prime_UTR 35.0%
! GAGAAATACATTAAGGGCAT+TGG + chr5_2:35613601-35613620 Msa0768830:three_prime_UTR 35.0%
CAATCTTACCACATTGTTTC+TGG + chr5_2:35613625-35613644 Msa0768830:three_prime_UTR 35.0%
! TACACTGGAAGATCAGTTTT+AGG + chr5_2:35612881-35612900 Msa0768830:CDS 35.0%
! ACACTGGAAGATCAGTTTTA+GGG + chr5_2:35612882-35612901 Msa0768830:CDS 35.0%
GATATTTGAATTGGTGGAAC+TGG - chr5_2:35612914-35612933 None:intergenic 35.0%
CATCTTTGGATTCAACAATG+GGG + chr5_2:35612933-35612952 Msa0768830:CDS 35.0%
TTGCATTGCACTAAAGGAAA+AGG + chr5_2:35613022-35613041 Msa0768830:CDS 35.0%
GCAATATTCTGGACAAGATT+CGG + chr5_2:35613177-35613196 Msa0768830:CDS 35.0%
TAGAAGATCAGATGAATCGA+TGG - chr5_2:35613290-35613309 None:intergenic 35.0%
ACAATGCAATTAGGGGAATT+TGG + chr5_2:35613364-35613383 Msa0768830:CDS 35.0%
! CCCATCTTTTGAATATCTTC+AGG - chr5_2:35613570-35613589 None:intergenic 35.0%
TCAAAAGATGGGATAACAAG+CGG + chr5_2:35613578-35613597 Msa0768830:exon 35.0%
AAGCGGGAGAAATACATTAA+GGG + chr5_2:35613595-35613614 Msa0768830:three_prime_UTR 35.0%
! GAGAAATACATTAAGGGCAT+TGG + chr5_2:35613601-35613620 Msa0768830:three_prime_UTR 35.0%
CAATCTTACCACATTGTTTC+TGG + chr5_2:35613625-35613644 Msa0768830:three_prime_UTR 35.0%
ATTTGAATTGGTGGAACTGG+TGG - chr5_2:35612911-35612930 None:intergenic 40.0%
! GATTCAACAATGGGGAGTAT+TGG + chr5_2:35612941-35612960 Msa0768830:CDS 40.0%
! AGTATTGGACAAGGATTGGT+TGG + chr5_2:35612956-35612975 Msa0768830:CDS 40.0%
TAGTGCAATGCAACGATGAA+TGG - chr5_2:35613015-35613034 None:intergenic 40.0%
TCATCGTTGCATTGCACTAA+AGG + chr5_2:35613016-35613035 Msa0768830:CDS 40.0%
CCACTACTTGGTAAACTTGA+TGG - chr5_2:35613061-35613080 None:intergenic 40.0%
CCATCAAGTTTACCAAGTAG+TGG + chr5_2:35613058-35613077 Msa0768830:CDS 40.0%
ACCTGCATACTTTGATACCT+CGG - chr5_2:35613127-35613146 None:intergenic 40.0%
AGATCAGATGAATCGATGGT+GGG - chr5_2:35613286-35613305 None:intergenic 40.0%
AAGATCAGATGAATCGATGG+TGG - chr5_2:35613287-35613306 None:intergenic 40.0%
TAATTGCATTGTCGAAGCAC+CGG - chr5_2:35613356-35613375 None:intergenic 40.0%
GTGCTTCGACAATGCAATTA+GGG + chr5_2:35613356-35613375 Msa0768830:CDS 40.0%
TGCTTCGACAATGCAATTAG+GGG + chr5_2:35613357-35613376 Msa0768830:CDS 40.0%
ATTAGGGGAATTTGGATTCG+AGG + chr5_2:35613372-35613391 Msa0768830:CDS 40.0%
TTAGGGGAATTTGGATTCGA+GGG + chr5_2:35613373-35613392 Msa0768830:CDS 40.0%
CAAAAGATGGGATAACAAGC+GGG + chr5_2:35613579-35613598 Msa0768830:exon 40.0%
CAAGCGGGAGAAATACATTA+AGG + chr5_2:35613594-35613613 Msa0768830:three_prime_UTR 40.0%
TTGGTTGTCCAGAAACAATG+TGG - chr5_2:35613636-35613655 Msa0768840:CDS 40.0%
ATTTGAATTGGTGGAACTGG+TGG - chr5_2:35612911-35612930 None:intergenic 40.0%
! GATTCAACAATGGGGAGTAT+TGG + chr5_2:35612941-35612960 Msa0768830:CDS 40.0%
! AGTATTGGACAAGGATTGGT+TGG + chr5_2:35612956-35612975 Msa0768830:CDS 40.0%
TAGTGCAATGCAACGATGAA+TGG - chr5_2:35613015-35613034 None:intergenic 40.0%
TCATCGTTGCATTGCACTAA+AGG + chr5_2:35613016-35613035 Msa0768830:CDS 40.0%
CCACTACTTGGTAAACTTGA+TGG - chr5_2:35613061-35613080 None:intergenic 40.0%
CCATCAAGTTTACCAAGTAG+TGG + chr5_2:35613058-35613077 Msa0768830:CDS 40.0%
ACCTGCATACTTTGATACCT+CGG - chr5_2:35613127-35613146 None:intergenic 40.0%
AGATCAGATGAATCGATGGT+GGG - chr5_2:35613286-35613305 None:intergenic 40.0%
AAGATCAGATGAATCGATGG+TGG - chr5_2:35613287-35613306 None:intergenic 40.0%
TAATTGCATTGTCGAAGCAC+CGG - chr5_2:35613356-35613375 None:intergenic 40.0%
GTGCTTCGACAATGCAATTA+GGG + chr5_2:35613356-35613375 Msa0768830:CDS 40.0%
TGCTTCGACAATGCAATTAG+GGG + chr5_2:35613357-35613376 Msa0768830:CDS 40.0%
ATTAGGGGAATTTGGATTCG+AGG + chr5_2:35613372-35613391 Msa0768830:CDS 40.0%
TTAGGGGAATTTGGATTCGA+GGG + chr5_2:35613373-35613392 Msa0768830:CDS 40.0%
CAAAAGATGGGATAACAAGC+GGG + chr5_2:35613579-35613598 Msa0768830:exon 40.0%
CAAGCGGGAGAAATACATTA+AGG + chr5_2:35613594-35613613 Msa0768830:three_prime_UTR 40.0%
TTGGTTGTCCAGAAACAATG+TGG - chr5_2:35613636-35613655 Msa0768840:CDS 40.0%
!! ACAATGGGGAGTATTGGACA+AGG + chr5_2:35612947-35612966 Msa0768830:CDS 45.0%
ATCAACAACGGACCACTACT+TGG - chr5_2:35613073-35613092 None:intergenic 45.0%
! TCCGAGGTATCAAAGTATGC+AGG + chr5_2:35613123-35613142 Msa0768830:CDS 45.0%
AGTATGCAGGTCGATACCTT+TGG + chr5_2:35613136-35613155 Msa0768830:CDS 45.0%
ATGTCGCATGCGCAATATTC+TGG + chr5_2:35613166-35613185 Msa0768830:CDS 45.0%
GATCAGATGAATCGATGGTG+GGG - chr5_2:35613285-35613304 None:intergenic 45.0%
CTAATGAAGTGGTGTAACCC+GGG - chr5_2:35613327-35613346 None:intergenic 45.0%
CCTAATGAAGTGGTGTAACC+CGG - chr5_2:35613328-35613347 None:intergenic 45.0%
ACCACTTCATTAGGTGAGAC+CGG + chr5_2:35613334-35613353 Msa0768830:CDS 45.0%
GGTGCTTCGACAATGCAATT+AGG + chr5_2:35613355-35613374 Msa0768830:CDS 45.0%
GAAACTGGTACTTACTCCCA+CGG - chr5_2:35613409-35613428 None:intergenic 45.0%
!! ACAATGGGGAGTATTGGACA+AGG + chr5_2:35612947-35612966 Msa0768830:CDS 45.0%
ATCAACAACGGACCACTACT+TGG - chr5_2:35613073-35613092 None:intergenic 45.0%
! TCCGAGGTATCAAAGTATGC+AGG + chr5_2:35613123-35613142 Msa0768830:CDS 45.0%
AGTATGCAGGTCGATACCTT+TGG + chr5_2:35613136-35613155 Msa0768830:CDS 45.0%
ATGTCGCATGCGCAATATTC+TGG + chr5_2:35613166-35613185 Msa0768830:CDS 45.0%
GATCAGATGAATCGATGGTG+GGG - chr5_2:35613285-35613304 None:intergenic 45.0%
CTAATGAAGTGGTGTAACCC+GGG - chr5_2:35613327-35613346 None:intergenic 45.0%
CCTAATGAAGTGGTGTAACC+CGG - chr5_2:35613328-35613347 None:intergenic 45.0%
ACCACTTCATTAGGTGAGAC+CGG + chr5_2:35613334-35613353 Msa0768830:CDS 45.0%
GGTGCTTCGACAATGCAATT+AGG + chr5_2:35613355-35613374 Msa0768830:CDS 45.0%
GAAACTGGTACTTACTCCCA+CGG - chr5_2:35613409-35613428 None:intergenic 45.0%
!! TCATATAATTTAAAAATAAA+AGG - chr5_2:35613480-35613499 None:intergenic 5.0%
!! TCATATAATTTAAAAATAAA+AGG - chr5_2:35613480-35613499 None:intergenic 5.0%
!! GGGGAGTATTGGACAAGGAT+TGG + chr5_2:35612952-35612971 Msa0768830:CDS 50.0%
CTCGGAGTCCATGATTGTTG+AGG - chr5_2:35613109-35613128 None:intergenic 50.0%
CTCAACAATCATGGACTCCG+AGG + chr5_2:35613107-35613126 Msa0768830:CDS 50.0%
ATGCGACATTGCGTTGCCAA+AGG - chr5_2:35613155-35613174 None:intergenic 50.0%
CCGGGTTACACCACTTCATT+AGG + chr5_2:35613325-35613344 Msa0768830:CDS 50.0%
ACCGGTCTCACCTAATGAAG+TGG - chr5_2:35613338-35613357 None:intergenic 50.0%
!! TCGAGGGTTTTGATGATCCG+TGG + chr5_2:35613389-35613408 Msa0768830:CDS 50.0%
!! CGAGGGTTTTGATGATCCGT+GGG + chr5_2:35613390-35613409 Msa0768830:CDS 50.0%
!! GGGGAGTATTGGACAAGGAT+TGG + chr5_2:35612952-35612971 Msa0768830:CDS 50.0%
CTCGGAGTCCATGATTGTTG+AGG - chr5_2:35613109-35613128 None:intergenic 50.0%
CTCAACAATCATGGACTCCG+AGG + chr5_2:35613107-35613126 Msa0768830:CDS 50.0%
ATGCGACATTGCGTTGCCAA+AGG - chr5_2:35613155-35613174 None:intergenic 50.0%
CCGGGTTACACCACTTCATT+AGG + chr5_2:35613325-35613344 Msa0768830:CDS 50.0%
ACCGGTCTCACCTAATGAAG+TGG - chr5_2:35613338-35613357 None:intergenic 50.0%
!! TCGAGGGTTTTGATGATCCG+TGG + chr5_2:35613389-35613408 Msa0768830:CDS 50.0%
!! CGAGGGTTTTGATGATCCGT+GGG + chr5_2:35613390-35613409 Msa0768830:CDS 50.0%
TCTACTCCCCACCACTGTCC+CGG + chr5_2:35613306-35613325 Msa0768830:CDS 60.0%
TGTAACCCGGGACAGTGGTG+GGG - chr5_2:35613315-35613334 None:intergenic 60.0%
GTGTAACCCGGGACAGTGGT+GGG - chr5_2:35613316-35613335 None:intergenic 60.0%
AGTGGTGTAACCCGGGACAG+TGG - chr5_2:35613320-35613339 None:intergenic 60.0%
TCTACTCCCCACCACTGTCC+CGG + chr5_2:35613306-35613325 Msa0768830:CDS 60.0%
TGTAACCCGGGACAGTGGTG+GGG - chr5_2:35613315-35613334 None:intergenic 60.0%
GTGTAACCCGGGACAGTGGT+GGG - chr5_2:35613316-35613335 None:intergenic 60.0%
AGTGGTGTAACCCGGGACAG+TGG - chr5_2:35613320-35613339 None:intergenic 60.0%
CTACTCCCCACCACTGTCCC+GGG + chr5_2:35613307-35613326 Msa0768830:CDS 65.0%
GGTGTAACCCGGGACAGTGG+TGG - chr5_2:35613317-35613336 None:intergenic 65.0%
CTACTCCCCACCACTGTCCC+GGG + chr5_2:35613307-35613326 Msa0768830:CDS 65.0%
GGTGTAACCCGGGACAGTGG+TGG - chr5_2:35613317-35613336 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 35612851 35613889 35612851 ID=Msa0768830;Name=Msa0768830
chr5_2 mRNA 35612851 35613889 35612851 ID=Msa0768830-mRNA-1;Parent=Msa0768830;Name=Msa0768830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|1|1|2|296|209
chr5_2 exon 35612851 35613413 35612851 ID=Msa0768830-mRNA-1:exon:12092;Parent=Msa0768830-mRNA-1
chr5_2 exon 35613527 35613889 35613527 ID=Msa0768830-mRNA-1:exon:12093;Parent=Msa0768830-mRNA-1
chr5_2 CDS 35612851 35613413 35612851 ID=Msa0768830-mRNA-1:cds;Parent=Msa0768830-mRNA-1
chr5_2 CDS 35613527 35613593 35613527 ID=Msa0768830-mRNA-1:cds;Parent=Msa0768830-mRNA-1
chr5_2 three_prime_UTR 35613594 35613889 35613594 ID=Msa0768830-mRNA-1:three_prime_utr;Parent=Msa0768830-mRNA-1
Gene Sequence

>Msa0768830

CTTTATAAAGAATCTAGCATTATATCTGAATACACTGGAAGATCAGTTTTAGGGTTTCCACCAGTTCCACCAATTCAAATATCATCTTTGGATTCAACAATGGGGAGTATTGGACAAGGATTGGTTGGAAATTCATTTGATATTGGTGAAATTCATAACAACCATTCATCGTTGCATTGCACTAAAGGAAAAGGAAAACTCTTCAATCCATCAAGTTTACCAAGTAGTGGTCCGTTGTTGATGAATGAACAATTACCTCAACAATCATGGACTCCGAGGTATCAAAGTATGCAGGTCGATACCTTTGGCAACGCAATGTCGCATGCGCAATATTCTGGACAAGATTCGGCAATTGTGCCCCCACATTTTGATTTAGATGTCCAAAATCATATTTTCTCAGGTGTCAATATTGATTCAACTGATCTTCTACTCCCCACCATCGATTCATCTGATCTTCTACTCCCCACCACTGTCCCGGGTTACACCACTTCATTAGGTGAGACCGGTGCTTCGACAATGCAATTAGGGGAATTTGGATTCGAGGGTTTTGATGATCCGTGGGAATCATTCTTGAATAGCATTTGGCATAATGAAATACTTTCACCTGAAGATATTCAAAAGATGGGATAA
Protein sequence

>Msa0768830

LYKESSIISEYTGRSVLGFPPVPPIQISSLDSTMGSIGQGLVGNSFDIGEIHNNHSSLHCTKGKGKLFNPSSLPSSGPLLMNEQLPQQSWTPRYQSMQVDTFGNAMSHAQYSGQDSAIVPPHFDLDVQNHIFSGVNIDSTDLLLPTIDSSDLLLPTTVPGYTTSLGETGASTMQLGEFGFEGFDDPWESFLNSIWHNEILSPEDIQKMG*