AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0770320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0770320 MtrunA17_Chr5g0447981 95.062 162 7 1 1 161 1 162 2.08e-111 313
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0770320 AT2G04790.15 60.000 145 55 2 15 156 34 178 2.59e-62 190
Msa0770320 AT2G04790.10 60.000 145 55 2 15 156 36 180 2.61e-62 190
Msa0770320 AT2G04790.2 60.000 145 55 2 15 156 17 161 3.68e-62 189
Msa0770320 AT2G04790.13 63.636 132 45 2 15 143 36 167 2.23e-60 185
Msa0770320 AT2G04790.14 63.636 132 45 2 15 143 34 165 2.72e-60 184
Msa0770320 AT2G04790.1 63.636 132 45 2 15 143 17 148 3.33e-60 184
Msa0770320 AT2G04790.9 63.636 77 26 1 15 89 36 112 2.27e-31 109
Msa0770320 AT2G04790.12 63.636 77 26 1 15 89 36 112 2.27e-31 109
Msa0770320 AT2G04790.3 63.636 77 26 1 15 89 17 93 3.80e-31 108
Msa0770320 AT2G04790.4 63.636 77 26 1 15 89 17 93 3.80e-31 108
Msa0770320 AT2G04790.5 63.636 77 26 1 15 89 36 112 5.68e-31 108
Msa0770320 AT2G04790.6 63.636 77 26 1 15 89 36 112 5.68e-31 108
Msa0770320 AT2G04790.16 63.636 77 26 1 15 89 17 93 8.30e-31 108
Msa0770320 AT2G04790.8 72.727 44 12 0 15 58 36 79 3.64e-18 75.1
Msa0770320 AT2G04790.11 72.727 44 12 0 15 58 36 79 3.64e-18 75.1
Msa0770320 AT2G04790.7 72.727 44 12 0 15 58 36 79 3.64e-18 75.1

Find 58 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTACTCCCTTAATATT+TGG 0.106470 5_2:-40854275 Msa0770320:CDS
ATAAAGAGTTATTATGTATT+TGG 0.136701 5_2:+40853126 None:intergenic
ATCACTTTCCTCGATTTATT+TGG 0.194419 5_2:+40853214 None:intergenic
GCTGCAAATCCCAAATATTA+AGG 0.224867 5_2:+40854265 None:intergenic
TGCTTACTCCCTTAATATTT+GGG 0.251158 5_2:-40854274 Msa0770320:CDS
TACATATCTTCAGGATCTTT+TGG 0.289471 5_2:+40854036 None:intergenic
CAACTACCAGATTTAGATTC+TGG 0.293603 5_2:+40852935 None:intergenic
ATTCTGGATCTGAACTAATA+TGG 0.297613 5_2:+40852951 None:intergenic
GGTTTGGTCAGACAACTTAT+TGG 0.315958 5_2:-40853519 Msa0770320:CDS
ATGTGGCAGCAATGCCTCTA+AGG 0.323320 5_2:-40854332 Msa0770320:CDS
GACATTGAGTTGCTTTCCTC+TGG 0.325894 5_2:-40853445 Msa0770320:three_prime_UTR
AGTTATTATGTATTTGGAAT+AGG 0.336155 5_2:+40853132 None:intergenic
CCTCGATTTATTTGGCTTTG+AGG 0.364586 5_2:+40853222 None:intergenic
TTGGGATTTGCAGCATTTCA+TGG 0.365079 5_2:-40854256 Msa0770320:CDS
AATTTCTTCTCTTATATAAG+AGG 0.389578 5_2:+40852981 None:intergenic
GGTCCCTTTGAAGCCCTTAG+AGG 0.394810 5_2:+40854318 None:intergenic
TAAGTTTCTCTTACAAAAGT+AGG 0.398995 5_2:-40854447 Msa0770320:five_prime_UTR
TTCTCTTACAAAAGTAGGAT+TGG 0.405965 5_2:-40854442 Msa0770320:five_prime_UTR
TAAATCGAGGAAAGTGATCT+TGG 0.409500 5_2:-40853209 Msa0770320:three_prime_UTR
ATAAACATGTCCTAAGGAAC+TGG 0.422901 5_2:-40852881 Msa0770320:three_prime_UTR
CAAACCTTCCTCACCTTCAA+AGG 0.430343 5_2:-40854226 Msa0770320:intron
TGTAGCAAACACTTAACATA+TGG 0.431558 5_2:-40853090 Msa0770320:three_prime_UTR
CTGCAAATCCCAAATATTAA+GGG 0.434798 5_2:+40854266 None:intergenic
AGTTGCTTTCCTCTGGATCA+TGG 0.435566 5_2:-40853438 Msa0770320:intron
TCTCTTGAGTGACATAGAAA+TGG 0.440683 5_2:-40854403 Msa0770320:exon
CGAAGCAAATGTTGGCTAGT+TGG 0.443608 5_2:-40853830 Msa0770320:CDS
TACATAGTATTTCATCCATA+TGG 0.446699 5_2:-40852906 Msa0770320:three_prime_UTR
GAACAACACTCCAGTTCCTT+AGG 0.449583 5_2:+40852871 None:intergenic
AATAGGATTGAAACCTTTGA+AGG 0.455552 5_2:+40854213 None:intergenic
AGGACATGTTTATCACCATA+TGG 0.457084 5_2:+40852891 None:intergenic
TCAGATCCAGAATCTAAATC+TGG 0.470856 5_2:-40852941 Msa0770320:three_prime_UTR
AATGCCTCTAAGGGCTTCAA+AGG 0.472734 5_2:-40854322 Msa0770320:CDS
TGGGAAACAGATTTGAGAAT+CGG 0.477510 5_2:-40853758 Msa0770320:CDS
ACTAGCCAACATTTGCTTCG+CGG 0.493748 5_2:+40853833 None:intergenic
AGTGCCGCATACATATCTTC+AGG 0.506500 5_2:+40854027 None:intergenic
GAAACCTTTGAAGGTGAGGA+AGG 0.532186 5_2:+40854222 None:intergenic
AGGCAGAGAGGAAGTGTATG+TGG 0.544412 5_2:-40854349 Msa0770320:CDS
TTATCTGGTAGAGCTGTGCC+AGG 0.545939 5_2:-40853997 Msa0770320:intron
TCAAAGACAAGAATGGTAAG+AGG 0.550312 5_2:-40854369 Msa0770320:CDS
AGTTGTATCAAAGACAAGAA+TGG 0.561294 5_2:-40854376 Msa0770320:CDS
TGTGGCAGCAATGCCTCTAA+GGG 0.570128 5_2:-40854331 Msa0770320:CDS
GATTGTCGCGATTATACCAA+TGG 0.578050 5_2:-40853728 Msa0770320:intron
ATGCCTCTAAGGGCTTCAAA+GGG 0.584284 5_2:-40854321 Msa0770320:CDS
TCATGATCATCTAGTTACCC+TGG 0.586008 5_2:+40853466 None:intergenic
ATGGTGATAAACATGTCCTA+AGG 0.593739 5_2:-40852887 Msa0770320:three_prime_UTR
GATTGAAACCTTTGAAGGTG+AGG 0.593749 5_2:+40854218 None:intergenic
GATTGCCGCGAAGCAAATGT+TGG 0.594551 5_2:-40853838 Msa0770320:CDS
GCAGCAGACATAAGCAATTG+TGG 0.599579 5_2:+40854294 None:intergenic
TCACAGGAGCTGTTCTTGTG+AGG 0.601866 5_2:-40853871 Msa0770320:intron
GAGGAAAGTGATCTTGGACA+TGG 0.610289 5_2:-40853203 Msa0770320:three_prime_UTR
AAGAATGGTAAGAGGCAGAG+AGG 0.650232 5_2:-40854361 Msa0770320:CDS
CCTCAAAGCCAAATAAATCG+AGG 0.665555 5_2:-40853222 Msa0770320:three_prime_UTR
ATATGCAGAAGCAGATGCTG+TGG 0.680638 5_2:-40853807 Msa0770320:CDS
AGATCCTGAAGATATGTATG+CGG 0.681453 5_2:-40854031 Msa0770320:CDS
GCTGAAATGTTTGAGAAACC+AGG 0.686204 5_2:-40853484 Msa0770320:CDS
CTGAAATGTTTGAGAAACCA+GGG 0.687722 5_2:-40853483 Msa0770320:CDS
GAAATCATACCATGATCCAG+AGG 0.692733 5_2:+40853429 None:intergenic
GCAGACATAAGCAATTGTGG+TGG 0.708742 5_2:+40854297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCGTTAAAAAAAAGTTT+AGG - chr5_2:40853139-40853158 Msa0770320:three_prime_UTR 15.0%
!! ATAAAGAGTTATTATGTATT+TGG + chr5_2:40854181-40854200 None:intergenic 15.0%
!!! TTTTCGTTAAAAAAAAGTTT+AGG - chr5_2:40853139-40853158 Msa0770320:three_prime_UTR 15.0%
!! ATAAAGAGTTATTATGTATT+TGG + chr5_2:40854181-40854200 None:intergenic 15.0%
!! AAGTGAATTCAACAAAAAAT+GGG - chr5_2:40853527-40853546 Msa0770320:intron 20.0%
!! AACAACATATTAATTACCAT+TGG + chr5_2:40853595-40853614 None:intergenic 20.0%
!! TGATAATATACAAACACTTA+TGG + chr5_2:40853674-40853693 None:intergenic 20.0%
!!! TTCAATCTGAATTGAATTTT+TGG - chr5_2:40853752-40853771 Msa0770320:CDS 20.0%
!!! TGAATTTTTGGTCAATTTAA+AGG - chr5_2:40853764-40853783 Msa0770320:CDS 20.0%
!!! AGTTATTATGTATTTGGAAT+AGG + chr5_2:40854175-40854194 None:intergenic 20.0%
!! AATTTCTTCTCTTATATAAG+AGG + chr5_2:40854326-40854345 None:intergenic 20.0%
!! AAGTGAATTCAACAAAAAAT+GGG - chr5_2:40853527-40853546 Msa0770320:intron 20.0%
!! AACAACATATTAATTACCAT+TGG + chr5_2:40853595-40853614 None:intergenic 20.0%
!! TGATAATATACAAACACTTA+TGG + chr5_2:40853674-40853693 None:intergenic 20.0%
!!! TTCAATCTGAATTGAATTTT+TGG - chr5_2:40853752-40853771 Msa0770320:CDS 20.0%
!!! TGAATTTTTGGTCAATTTAA+AGG - chr5_2:40853764-40853783 Msa0770320:CDS 20.0%
!!! AGTTATTATGTATTTGGAAT+AGG + chr5_2:40854175-40854194 None:intergenic 20.0%
!! AATTTCTTCTCTTATATAAG+AGG + chr5_2:40854326-40854345 None:intergenic 20.0%
! TAAGTTTCTCTTACAAAAGT+AGG - chr5_2:40852857-40852876 Msa0770320:three_prime_UTR 25.0%
!!! TATTGCTTGTACTTATTTTC+AGG - chr5_2:40853225-40853244 Msa0770320:three_prime_UTR 25.0%
! GTGAATTATATGAACAGAAT+TGG + chr5_2:40853417-40853436 None:intergenic 25.0%
! ATTCTGTTCATATAATTCAC+AGG - chr5_2:40853417-40853436 Msa0770320:intron 25.0%
! CAAGTGAATTCAACAAAAAA+TGG - chr5_2:40853526-40853545 Msa0770320:intron 25.0%
!! TTTTGGTCAATTTAAAGGTT+TGG - chr5_2:40853769-40853788 Msa0770320:CDS 25.0%
!! ACATGTTGACATATAGTTTT+TGG + chr5_2:40853985-40854004 None:intergenic 25.0%
! TACATAGTATTTCATCCATA+TGG - chr5_2:40854398-40854417 Msa0770320:exon 25.0%
! TAAGTTTCTCTTACAAAAGT+AGG - chr5_2:40852857-40852876 Msa0770320:three_prime_UTR 25.0%
!!! TATTGCTTGTACTTATTTTC+AGG - chr5_2:40853225-40853244 Msa0770320:three_prime_UTR 25.0%
! GTGAATTATATGAACAGAAT+TGG + chr5_2:40853417-40853436 None:intergenic 25.0%
! ATTCTGTTCATATAATTCAC+AGG - chr5_2:40853417-40853436 Msa0770320:intron 25.0%
! CAAGTGAATTCAACAAAAAA+TGG - chr5_2:40853526-40853545 Msa0770320:intron 25.0%
!! TTTTGGTCAATTTAAAGGTT+TGG - chr5_2:40853769-40853788 Msa0770320:CDS 25.0%
!! ACATGTTGACATATAGTTTT+TGG + chr5_2:40853985-40854004 None:intergenic 25.0%
! TACATAGTATTTCATCCATA+TGG - chr5_2:40854398-40854417 Msa0770320:exon 25.0%
TTCTCTTACAAAAGTAGGAT+TGG - chr5_2:40852862-40852881 Msa0770320:three_prime_UTR 30.0%
AGTTGTATCAAAGACAAGAA+TGG - chr5_2:40852928-40852947 Msa0770320:three_prime_UTR 30.0%
TGCTTACTCCCTTAATATTT+GGG - chr5_2:40853030-40853049 Msa0770320:three_prime_UTR 30.0%
CTGCAAATCCCAAATATTAA+GGG + chr5_2:40853041-40853060 None:intergenic 30.0%
AATAGGATTGAAACCTTTGA+AGG + chr5_2:40853094-40853113 None:intergenic 30.0%
! ATTCCTCTCCTACATTTTAT+CGG - chr5_2:40853115-40853134 Msa0770320:three_prime_UTR 30.0%
ACGAAAAACCGATAAAATGT+AGG + chr5_2:40853126-40853145 None:intergenic 30.0%
! TACATATCTTCAGGATCTTT+TGG + chr5_2:40853271-40853290 None:intergenic 30.0%
! TAACTTGTGAACTTTCTGAT+TGG - chr5_2:40853350-40853369 Msa0770320:intron 30.0%
ACATAACTCTATACACAGAT+AGG + chr5_2:40853646-40853665 None:intergenic 30.0%
ATTGAACTGTTTGAGAATCA+TGG - chr5_2:40853903-40853922 Msa0770320:intron 30.0%
TATGTCAACATGTAATTACG+AGG - chr5_2:40853992-40854011 Msa0770320:intron 30.0%
ATCACTTTCCTCGATTTATT+TGG + chr5_2:40854093-40854112 None:intergenic 30.0%
TGTAGCAAACACTTAACATA+TGG - chr5_2:40854214-40854233 Msa0770320:intron 30.0%
ATTCTGGATCTGAACTAATA+TGG + chr5_2:40854356-40854375 None:intergenic 30.0%
TTCTCTTACAAAAGTAGGAT+TGG - chr5_2:40852862-40852881 Msa0770320:three_prime_UTR 30.0%
AGTTGTATCAAAGACAAGAA+TGG - chr5_2:40852928-40852947 Msa0770320:three_prime_UTR 30.0%
TGCTTACTCCCTTAATATTT+GGG - chr5_2:40853030-40853049 Msa0770320:three_prime_UTR 30.0%
CTGCAAATCCCAAATATTAA+GGG + chr5_2:40853041-40853060 None:intergenic 30.0%
AATAGGATTGAAACCTTTGA+AGG + chr5_2:40853094-40853113 None:intergenic 30.0%
! ATTCCTCTCCTACATTTTAT+CGG - chr5_2:40853115-40853134 Msa0770320:three_prime_UTR 30.0%
ACGAAAAACCGATAAAATGT+AGG + chr5_2:40853126-40853145 None:intergenic 30.0%
! TACATATCTTCAGGATCTTT+TGG + chr5_2:40853271-40853290 None:intergenic 30.0%
! TAACTTGTGAACTTTCTGAT+TGG - chr5_2:40853350-40853369 Msa0770320:intron 30.0%
ACATAACTCTATACACAGAT+AGG + chr5_2:40853646-40853665 None:intergenic 30.0%
ATTGAACTGTTTGAGAATCA+TGG - chr5_2:40853903-40853922 Msa0770320:intron 30.0%
TATGTCAACATGTAATTACG+AGG - chr5_2:40853992-40854011 Msa0770320:intron 30.0%
ATCACTTTCCTCGATTTATT+TGG + chr5_2:40854093-40854112 None:intergenic 30.0%
TGTAGCAAACACTTAACATA+TGG - chr5_2:40854214-40854233 Msa0770320:intron 30.0%
ATTCTGGATCTGAACTAATA+TGG + chr5_2:40854356-40854375 None:intergenic 30.0%
TCTCTTGAGTGACATAGAAA+TGG - chr5_2:40852901-40852920 Msa0770320:three_prime_UTR 35.0%
TCAAAGACAAGAATGGTAAG+AGG - chr5_2:40852935-40852954 Msa0770320:three_prime_UTR 35.0%
CTGCTTACTCCCTTAATATT+TGG - chr5_2:40853029-40853048 Msa0770320:three_prime_UTR 35.0%
GCTGCAAATCCCAAATATTA+AGG + chr5_2:40853042-40853061 None:intergenic 35.0%
AATGTAGGAGAGGAATGAAT+AGG + chr5_2:40853111-40853130 None:intergenic 35.0%
AAACCGATAAAATGTAGGAG+AGG + chr5_2:40853121-40853140 None:intergenic 35.0%
AGATCCTGAAGATATGTATG+CGG - chr5_2:40853273-40853292 Msa0770320:intron 35.0%
AATTAAGTCCAAATCTCACC+TGG + chr5_2:40853328-40853347 None:intergenic 35.0%
TGGGAAACAGATTTGAGAAT+CGG - chr5_2:40853546-40853565 Msa0770320:intron 35.0%
CTGAAATGTTTGAGAAACCA+GGG - chr5_2:40853821-40853840 Msa0770320:CDS 35.0%
AATCATGGCATGAGATATAG+AGG - chr5_2:40853918-40853937 Msa0770320:intron 35.0%
! TCCATCTCTAATTTTCTCTG+AGG + chr5_2:40853944-40853963 None:intergenic 35.0%
TAAATCGAGGAAAGTGATCT+TGG - chr5_2:40854095-40854114 Msa0770320:intron 35.0%
TCAGATCCAGAATCTAAATC+TGG - chr5_2:40854363-40854382 Msa0770320:CDS 35.0%
CAACTACCAGATTTAGATTC+TGG + chr5_2:40854372-40854391 None:intergenic 35.0%
AGGACATGTTTATCACCATA+TGG + chr5_2:40854416-40854435 None:intergenic 35.0%
ATGGTGATAAACATGTCCTA+AGG - chr5_2:40854417-40854436 Msa0770320:five_prime_UTR 35.0%
ATAAACATGTCCTAAGGAAC+TGG - chr5_2:40854423-40854442 Msa0770320:five_prime_UTR 35.0%
TCTCTTGAGTGACATAGAAA+TGG - chr5_2:40852901-40852920 Msa0770320:three_prime_UTR 35.0%
TCAAAGACAAGAATGGTAAG+AGG - chr5_2:40852935-40852954 Msa0770320:three_prime_UTR 35.0%
CTGCTTACTCCCTTAATATT+TGG - chr5_2:40853029-40853048 Msa0770320:three_prime_UTR 35.0%
GCTGCAAATCCCAAATATTA+AGG + chr5_2:40853042-40853061 None:intergenic 35.0%
AATGTAGGAGAGGAATGAAT+AGG + chr5_2:40853111-40853130 None:intergenic 35.0%
AAACCGATAAAATGTAGGAG+AGG + chr5_2:40853121-40853140 None:intergenic 35.0%
AGATCCTGAAGATATGTATG+CGG - chr5_2:40853273-40853292 Msa0770320:intron 35.0%
AATTAAGTCCAAATCTCACC+TGG + chr5_2:40853328-40853347 None:intergenic 35.0%
TGGGAAACAGATTTGAGAAT+CGG - chr5_2:40853546-40853565 Msa0770320:intron 35.0%
CTGAAATGTTTGAGAAACCA+GGG - chr5_2:40853821-40853840 Msa0770320:CDS 35.0%
AATCATGGCATGAGATATAG+AGG - chr5_2:40853918-40853937 Msa0770320:intron 35.0%
! TCCATCTCTAATTTTCTCTG+AGG + chr5_2:40853944-40853963 None:intergenic 35.0%
TAAATCGAGGAAAGTGATCT+TGG - chr5_2:40854095-40854114 Msa0770320:intron 35.0%
TCAGATCCAGAATCTAAATC+TGG - chr5_2:40854363-40854382 Msa0770320:CDS 35.0%
CAACTACCAGATTTAGATTC+TGG + chr5_2:40854372-40854391 None:intergenic 35.0%
AGGACATGTTTATCACCATA+TGG + chr5_2:40854416-40854435 None:intergenic 35.0%
ATGGTGATAAACATGTCCTA+AGG - chr5_2:40854417-40854436 Msa0770320:five_prime_UTR 35.0%
ATAAACATGTCCTAAGGAAC+TGG - chr5_2:40854423-40854442 Msa0770320:five_prime_UTR 35.0%
TTGGGATTTGCAGCATTTCA+TGG - chr5_2:40853048-40853067 Msa0770320:three_prime_UTR 40.0%
! GATTGAAACCTTTGAAGGTG+AGG + chr5_2:40853089-40853108 None:intergenic 40.0%
GATTGTCGCGATTATACCAA+TGG - chr5_2:40853576-40853595 Msa0770320:intron 40.0%
! GGTTTGGTCAGACAACTTAT+TGG - chr5_2:40853785-40853804 Msa0770320:CDS 40.0%
GCTGAAATGTTTGAGAAACC+AGG - chr5_2:40853820-40853839 Msa0770320:CDS 40.0%
TCATGATCATCTAGTTACCC+TGG + chr5_2:40853841-40853860 None:intergenic 40.0%
GAAATCATACCATGATCCAG+AGG + chr5_2:40853878-40853897 None:intergenic 40.0%
GCCTCAGAGAAAATTAGAGA+TGG - chr5_2:40853940-40853959 Msa0770320:intron 40.0%
!! CCTCGATTTATTTGGCTTTG+AGG + chr5_2:40854085-40854104 None:intergenic 40.0%
CCTCAAAGCCAAATAAATCG+AGG - chr5_2:40854082-40854101 Msa0770320:intron 40.0%
TTGGGATTTGCAGCATTTCA+TGG - chr5_2:40853048-40853067 Msa0770320:three_prime_UTR 40.0%
! GATTGAAACCTTTGAAGGTG+AGG + chr5_2:40853089-40853108 None:intergenic 40.0%
GATTGTCGCGATTATACCAA+TGG - chr5_2:40853576-40853595 Msa0770320:intron 40.0%
! GGTTTGGTCAGACAACTTAT+TGG - chr5_2:40853785-40853804 Msa0770320:CDS 40.0%
GCTGAAATGTTTGAGAAACC+AGG - chr5_2:40853820-40853839 Msa0770320:CDS 40.0%
TCATGATCATCTAGTTACCC+TGG + chr5_2:40853841-40853860 None:intergenic 40.0%
GAAATCATACCATGATCCAG+AGG + chr5_2:40853878-40853897 None:intergenic 40.0%
GCCTCAGAGAAAATTAGAGA+TGG - chr5_2:40853940-40853959 Msa0770320:intron 40.0%
!! CCTCGATTTATTTGGCTTTG+AGG + chr5_2:40854085-40854104 None:intergenic 40.0%
CCTCAAAGCCAAATAAATCG+AGG - chr5_2:40854082-40854101 Msa0770320:intron 40.0%
AAGAATGGTAAGAGGCAGAG+AGG - chr5_2:40852943-40852962 Msa0770320:three_prime_UTR 45.0%
! AATGCCTCTAAGGGCTTCAA+AGG - chr5_2:40852982-40853001 Msa0770320:three_prime_UTR 45.0%
! ATGCCTCTAAGGGCTTCAAA+GGG - chr5_2:40852983-40853002 Msa0770320:three_prime_UTR 45.0%
GCAGACATAAGCAATTGTGG+TGG + chr5_2:40853010-40853029 None:intergenic 45.0%
GCAGCAGACATAAGCAATTG+TGG + chr5_2:40853013-40853032 None:intergenic 45.0%
CAAACCTTCCTCACCTTCAA+AGG - chr5_2:40853078-40853097 Msa0770320:three_prime_UTR 45.0%
! GAAACCTTTGAAGGTGAGGA+AGG + chr5_2:40853085-40853104 None:intergenic 45.0%
TTAGACGCTCTCTAAGCTTG+AGG + chr5_2:40853197-40853216 None:intergenic 45.0%
AGTGCCGCATACATATCTTC+AGG + chr5_2:40853280-40853299 None:intergenic 45.0%
ACTAGCCAACATTTGCTTCG+CGG + chr5_2:40853474-40853493 None:intergenic 45.0%
! CGAAGCAAATGTTGGCTAGT+TGG - chr5_2:40853474-40853493 Msa0770320:exon 45.0%
ATATGCAGAAGCAGATGCTG+TGG - chr5_2:40853497-40853516 Msa0770320:CDS 45.0%
! GACATTGAGTTGCTTTCCTC+TGG - chr5_2:40853859-40853878 Msa0770320:CDS 45.0%
! AGTTGCTTTCCTCTGGATCA+TGG - chr5_2:40853866-40853885 Msa0770320:CDS 45.0%
GAGGAAAGTGATCTTGGACA+TGG - chr5_2:40854101-40854120 Msa0770320:intron 45.0%
GAACAACACTCCAGTTCCTT+AGG + chr5_2:40854436-40854455 None:intergenic 45.0%
AAGAATGGTAAGAGGCAGAG+AGG - chr5_2:40852943-40852962 Msa0770320:three_prime_UTR 45.0%
! AATGCCTCTAAGGGCTTCAA+AGG - chr5_2:40852982-40853001 Msa0770320:three_prime_UTR 45.0%
! ATGCCTCTAAGGGCTTCAAA+GGG - chr5_2:40852983-40853002 Msa0770320:three_prime_UTR 45.0%
GCAGACATAAGCAATTGTGG+TGG + chr5_2:40853010-40853029 None:intergenic 45.0%
GCAGCAGACATAAGCAATTG+TGG + chr5_2:40853013-40853032 None:intergenic 45.0%
CAAACCTTCCTCACCTTCAA+AGG - chr5_2:40853078-40853097 Msa0770320:three_prime_UTR 45.0%
! GAAACCTTTGAAGGTGAGGA+AGG + chr5_2:40853085-40853104 None:intergenic 45.0%
TTAGACGCTCTCTAAGCTTG+AGG + chr5_2:40853197-40853216 None:intergenic 45.0%
AGTGCCGCATACATATCTTC+AGG + chr5_2:40853280-40853299 None:intergenic 45.0%
ACTAGCCAACATTTGCTTCG+CGG + chr5_2:40853474-40853493 None:intergenic 45.0%
! CGAAGCAAATGTTGGCTAGT+TGG - chr5_2:40853474-40853493 Msa0770320:exon 45.0%
ATATGCAGAAGCAGATGCTG+TGG - chr5_2:40853497-40853516 Msa0770320:CDS 45.0%
! GACATTGAGTTGCTTTCCTC+TGG - chr5_2:40853859-40853878 Msa0770320:CDS 45.0%
! AGTTGCTTTCCTCTGGATCA+TGG - chr5_2:40853866-40853885 Msa0770320:CDS 45.0%
GAGGAAAGTGATCTTGGACA+TGG - chr5_2:40854101-40854120 Msa0770320:intron 45.0%
GAACAACACTCCAGTTCCTT+AGG + chr5_2:40854436-40854455 None:intergenic 45.0%
! AGGCAGAGAGGAAGTGTATG+TGG - chr5_2:40852955-40852974 Msa0770320:three_prime_UTR 50.0%
ATGTGGCAGCAATGCCTCTA+AGG - chr5_2:40852972-40852991 Msa0770320:three_prime_UTR 50.0%
TGTGGCAGCAATGCCTCTAA+GGG - chr5_2:40852973-40852992 Msa0770320:three_prime_UTR 50.0%
GTCCGAATGTTCACCATCGA+CGG - chr5_2:40853170-40853189 Msa0770320:three_prime_UTR 50.0%
GTCCGTCGATGGTGAACATT+CGG + chr5_2:40853175-40853194 None:intergenic 50.0%
!! GCGGCACTAGCAGTTTTATC+TGG - chr5_2:40853292-40853311 Msa0770320:intron 50.0%
TTATCTGGTAGAGCTGTGCC+AGG - chr5_2:40853307-40853326 Msa0770320:intron 50.0%
TCACAGGAGCTGTTCTTGTG+AGG - chr5_2:40853433-40853452 Msa0770320:intron 50.0%
GATTGCCGCGAAGCAAATGT+TGG - chr5_2:40853466-40853485 Msa0770320:exon 50.0%
! AGGCAGAGAGGAAGTGTATG+TGG - chr5_2:40852955-40852974 Msa0770320:three_prime_UTR 50.0%
ATGTGGCAGCAATGCCTCTA+AGG - chr5_2:40852972-40852991 Msa0770320:three_prime_UTR 50.0%
TGTGGCAGCAATGCCTCTAA+GGG - chr5_2:40852973-40852992 Msa0770320:three_prime_UTR 50.0%
GTCCGAATGTTCACCATCGA+CGG - chr5_2:40853170-40853189 Msa0770320:three_prime_UTR 50.0%
GTCCGTCGATGGTGAACATT+CGG + chr5_2:40853175-40853194 None:intergenic 50.0%
!! GCGGCACTAGCAGTTTTATC+TGG - chr5_2:40853292-40853311 Msa0770320:intron 50.0%
TTATCTGGTAGAGCTGTGCC+AGG - chr5_2:40853307-40853326 Msa0770320:intron 50.0%
TCACAGGAGCTGTTCTTGTG+AGG - chr5_2:40853433-40853452 Msa0770320:intron 50.0%
GATTGCCGCGAAGCAAATGT+TGG - chr5_2:40853466-40853485 Msa0770320:exon 50.0%
!! GGTCCCTTTGAAGCCCTTAG+AGG + chr5_2:40852989-40853008 None:intergenic 55.0%
CTAAGCTTGAGGTCCGTCGA+TGG + chr5_2:40853186-40853205 None:intergenic 55.0%
GAGCTGTGCCAGGTGAGATT+TGG - chr5_2:40853317-40853336 Msa0770320:intron 55.0%
!! GGTCCCTTTGAAGCCCTTAG+AGG + chr5_2:40852989-40853008 None:intergenic 55.0%
CTAAGCTTGAGGTCCGTCGA+TGG + chr5_2:40853186-40853205 None:intergenic 55.0%
GAGCTGTGCCAGGTGAGATT+TGG - chr5_2:40853317-40853336 Msa0770320:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 40852856 40854470 40852856 ID=Msa0770320;Name=Msa0770320
chr5_2 mRNA 40852856 40854470 40852856 ID=Msa0770320-mRNA-1;Parent=Msa0770320;Name=Msa0770320-mRNA-1;_AED=0.13;_eAED=0.13;_QI=64|1|1|1|0.75|0.8|5|446|161
chr5_2 exon 40852856 40853264 40852856 ID=Msa0770320-mRNA-1:exon:12941;Parent=Msa0770320-mRNA-1
chr5_2 exon 40853439 40853540 40853439 ID=Msa0770320-mRNA-1:exon:12940;Parent=Msa0770320-mRNA-1
chr5_2 exon 40853729 40853887 40853729 ID=Msa0770320-mRNA-1:exon:12939;Parent=Msa0770320-mRNA-1
chr5_2 exon 40853998 40854079 40853998 ID=Msa0770320-mRNA-1:exon:12938;Parent=Msa0770320-mRNA-1
chr5_2 exon 40854227 40854470 40854227 ID=Msa0770320-mRNA-1:exon:12937;Parent=Msa0770320-mRNA-1
chr5_2 five_prime_UTR 40854407 40854470 40854407 ID=Msa0770320-mRNA-1:five_prime_utr;Parent=Msa0770320-mRNA-1
chr5_2 CDS 40854227 40854406 40854227 ID=Msa0770320-mRNA-1:cds;Parent=Msa0770320-mRNA-1
chr5_2 CDS 40853998 40854079 40853998 ID=Msa0770320-mRNA-1:cds;Parent=Msa0770320-mRNA-1
chr5_2 CDS 40853729 40853887 40853729 ID=Msa0770320-mRNA-1:cds;Parent=Msa0770320-mRNA-1
chr5_2 CDS 40853476 40853540 40853476 ID=Msa0770320-mRNA-1:cds;Parent=Msa0770320-mRNA-1
chr5_2 three_prime_UTR 40853439 40853475 40853439 ID=Msa0770320-mRNA-1:three_prime_utr;Parent=Msa0770320-mRNA-1
chr5_2 three_prime_UTR 40852856 40853264 40852856 ID=Msa0770320-mRNA-1:three_prime_utr;Parent=Msa0770320-mRNA-1
Gene Sequence

>Msa0770320

ATGGAATCAGTTGTATCAAAGACAAGAATGGTAAGAGGCAGAGAGGAAGTGTATGTGGCAGCAATGCCTCTAAGGGCTTCAAAGGGACCACCACAATTGCTTATGTCTGCTGCTTACTCCCTTAATATTTGGGATTTGCAGCATTTCATGGTCATCATCAAACCTTCCTCACCTTCAAAGGTTCTAGTTTTTGATTTTCAACCAAAAGATCCTGAAGATATGTATGCGGCACTAGCAGTTTTATCTGGTAGAGCTGTGCCAGGAGCTGTTCTTGTGAGGAAGTTAAAAAGATTGCCGCGAAGCAAATGTTGGCTAGTTGGATATGCAGAAGCAGATGCTGTGGAAATAGCAAGTGAATTCAACAAAAAATGGGAAACAGATTTGAGAATCGGTGTTAATGATTGTCGCGATTATACCAATGGTTTGGTCAGACAACTTATTGGCGAAGAAGATGTGCTGAAATGTTTGAGAAACCAGGGTAACTAG
Protein sequence

>Msa0770320

MESVVSKTRMVRGREEVYVAAMPLRASKGPPQLLMSAAYSLNIWDLQHFMVIIKPSSPSKVLVFDFQPKDPEDMYAALAVLSGRAVPGAVLVRKLKRLPRSKCWLVGYAEADAVEIASEFNKKWETDLRIGVNDCRDYTNGLVRQLIGEEDVLKCLRNQGN*