AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0826330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826330 MtrunA17_Chr5g0447981 95.679 162 6 1 1 161 1 162 6.56e-112 314
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826330 AT2G04790.10 60.690 145 54 2 15 156 36 180 6.51e-63 191
Msa0826330 AT2G04790.15 60.690 145 54 2 15 156 34 178 6.81e-63 191
Msa0826330 AT2G04790.2 60.690 145 54 2 15 156 17 161 9.90e-63 190
Msa0826330 AT2G04790.13 64.394 132 44 2 15 143 36 167 6.42e-61 186
Msa0826330 AT2G04790.14 64.394 132 44 2 15 143 34 165 7.25e-61 186
Msa0826330 AT2G04790.1 64.394 132 44 2 15 143 17 148 1.05e-60 185
Msa0826330 AT2G04790.9 64.935 77 25 1 15 89 36 112 6.85e-32 110
Msa0826330 AT2G04790.12 64.935 77 25 1 15 89 36 112 6.85e-32 110
Msa0826330 AT2G04790.3 64.935 77 25 1 15 89 17 93 1.24e-31 109
Msa0826330 AT2G04790.4 64.935 77 25 1 15 89 17 93 1.24e-31 109
Msa0826330 AT2G04790.5 64.935 77 25 1 15 89 36 112 1.80e-31 110
Msa0826330 AT2G04790.6 64.935 77 25 1 15 89 36 112 1.80e-31 110
Msa0826330 AT2G04790.16 64.935 77 25 1 15 89 17 93 2.83e-31 109
Msa0826330 AT2G04790.8 72.727 44 12 0 15 58 36 79 3.60e-18 75.1
Msa0826330 AT2G04790.11 72.727 44 12 0 15 58 36 79 3.60e-18 75.1
Msa0826330 AT2G04790.7 72.727 44 12 0 15 58 36 79 3.60e-18 75.1

Find 57 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTACTCCCTTAATATT+TGG 0.106470 5_3:+66668306 Msa0826330:CDS
ATAAAGAGTTATTATGTATT+TGG 0.136701 5_3:-66669442 None:intergenic
ATCACTTTCCTCGATTTATT+TGG 0.194419 5_3:-66669354 None:intergenic
GCTGCAAATCCCAAATATTA+AGG 0.224867 5_3:-66668316 None:intergenic
TGCTTACTCCCTTAATATTT+GGG 0.251158 5_3:+66668307 Msa0826330:CDS
CAACTACCAGATTTAGATTC+TGG 0.293603 5_3:-66669633 None:intergenic
ATTCTGGATCTGAACTAATA+TGG 0.297613 5_3:-66669617 None:intergenic
TATATATCTTCAGGATCTTT+TGG 0.303960 5_3:-66668543 None:intergenic
GGTTTGGTCAGACAACTTAT+TGG 0.315958 5_3:+66669060 Msa0826330:intron
ATGTGGCAGCAATGCCTCTA+AGG 0.323320 5_3:+66668249 Msa0826330:CDS
AGTTATTATGTATTTGGAAT+AGG 0.336155 5_3:-66669436 None:intergenic
CCTCGATTTATTTGGCTTTG+AGG 0.364586 5_3:-66669346 None:intergenic
TTGGGATTTGCAGCATTTCA+TGG 0.365079 5_3:+66668325 Msa0826330:CDS
TACATTGTATTTCATCCATA+TGG 0.387239 5_3:+66669662 Msa0826330:three_prime_UTR
AATTTCTTCTCTTATATAAG+AGG 0.389578 5_3:-66669587 None:intergenic
GGTCCCTTTGAAGCCCTTAG+AGG 0.394810 5_3:-66668263 None:intergenic
TAAGTTTCTCTTACAAAAGT+AGG 0.398995 5_3:+66668134 Msa0826330:five_prime_UTR
TTCTCTTACAAAAGTAGGAT+TGG 0.405965 5_3:+66668139 Msa0826330:five_prime_UTR
TAAATCGAGGAAAGTGATCT+TGG 0.409500 5_3:+66669359 Msa0826330:three_prime_UTR
ATAAACATGTCCTAAGGAAC+TGG 0.422901 5_3:+66669687 Msa0826330:three_prime_UTR
TAGATGATCATGACATTCTC+TGG 0.426557 5_3:+66669123 Msa0826330:exon
CAAACCTTCCTCACCTTCAA+AGG 0.430343 5_3:+66668355 Msa0826330:CDS
TGTAGCAAACACTTAACATA+TGG 0.431558 5_3:+66669478 Msa0826330:three_prime_UTR
CTGCAAATCCCAAATATTAA+GGG 0.434798 5_3:-66668315 None:intergenic
TCTCTTGAGTGACATAGAAA+TGG 0.440683 5_3:+66668178 Msa0826330:exon
CGAAGCAAATGTTGGCTAGT+TGG 0.443608 5_3:+66668749 Msa0826330:CDS
GAACAACACTCCAGTTCCTT+AGG 0.449583 5_3:-66669697 None:intergenic
AATAGGATTGAAACCTTTGA+AGG 0.455552 5_3:-66668368 None:intergenic
AGGACATGTTTATCACCATA+TGG 0.457084 5_3:-66669677 None:intergenic
TCAGATCCAGAATCTAAATC+TGG 0.470856 5_3:+66669627 Msa0826330:three_prime_UTR
AATGCCTCTAAGGGCTTCAA+AGG 0.472734 5_3:+66668259 Msa0826330:CDS
AGTGCCGCATATATATCTTC+AGG 0.473960 5_3:-66668552 None:intergenic
TGGGAAACAGATTTGAGAAT+CGG 0.477510 5_3:+66668821 Msa0826330:CDS
ACTAGCCAACATTTGCTTCG+CGG 0.493748 5_3:-66668746 None:intergenic
GAAACCTTTGAAGGTGAGGA+AGG 0.532186 5_3:-66668359 None:intergenic
TCATGACATTCTCTGGATCA+TGG 0.535394 5_3:+66669130 Msa0826330:three_prime_UTR
AGGCAGAGAGGAAGTGTATG+TGG 0.544412 5_3:+66668232 Msa0826330:CDS
TTATCTGGTAGAGCTGTGCC+AGG 0.545939 5_3:+66668582 Msa0826330:CDS
TCAAAGACAAGAATGGTAAG+AGG 0.550312 5_3:+66668212 Msa0826330:CDS
AGTTGTATCAAAGACAAGAA+TGG 0.561294 5_3:+66668205 Msa0826330:CDS
TGTGGCAGCAATGCCTCTAA+GGG 0.570128 5_3:+66668250 Msa0826330:CDS
GATTGTCGCGATTATACCAA+TGG 0.578050 5_3:+66668851 Msa0826330:CDS
ATGCCTCTAAGGGCTTCAAA+GGG 0.584284 5_3:+66668260 Msa0826330:CDS
TCATGATCATCTAGTTACCC+TGG 0.586008 5_3:-66669113 None:intergenic
ATGGTGATAAACATGTCCTA+AGG 0.593739 5_3:+66669681 Msa0826330:three_prime_UTR
GATTGAAACCTTTGAAGGTG+AGG 0.593749 5_3:-66668363 None:intergenic
GATTGCCGCGAAGCAAATGT+TGG 0.594551 5_3:+66668741 Msa0826330:CDS
GCAGCAGACATAAGCAATTG+TGG 0.599579 5_3:-66668287 None:intergenic
TCACAGGAGCTGTTCTTGTG+AGG 0.601866 5_3:+66668708 Msa0826330:intron
GAGGAAAGTGATCTTGGACA+TGG 0.610289 5_3:+66669365 Msa0826330:three_prime_UTR
AAGAATGGTAAGAGGCAGAG+AGG 0.650232 5_3:+66668220 Msa0826330:CDS
CCTCAAAGCCAAATAAATCG+AGG 0.665555 5_3:+66669346 Msa0826330:three_prime_UTR
ATATGCAGAAGCAGATGCTG+TGG 0.680638 5_3:+66668772 Msa0826330:CDS
AGATCCTGAAGATATATATG+CGG 0.681441 5_3:+66668548 Msa0826330:CDS
GCTGAAATGTTTGAGAAACC+AGG 0.686204 5_3:+66669095 Msa0826330:CDS
CTGAAATGTTTGAGAAACCA+GGG 0.687722 5_3:+66669096 Msa0826330:CDS
GCAGACATAAGCAATTGTGG+TGG 0.708742 5_3:-66668284 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCGTTAAAAAAAAGTTT+AGG + chr5_3:66668414-66668433 Msa0826330:intron 15.0%
!! ATAAAGAGTTATTATGTATT+TGG - chr5_3:66669445-66669464 None:intergenic 15.0%
!!! TTTTCGTTAAAAAAAAGTTT+AGG + chr5_3:66668414-66668433 Msa0826330:intron 15.0%
!! ATAAAGAGTTATTATGTATT+TGG - chr5_3:66669445-66669464 None:intergenic 15.0%
!!! TATTGCTAGTATTTATTTTC+AGG + chr5_3:66668500-66668519 Msa0826330:intron 20.0%
!! AAGTGAATTCAACAAAAAAT+GGG + chr5_3:66668802-66668821 Msa0826330:CDS 20.0%
!! AACAACATATTAATTACCAT+TGG - chr5_3:66668870-66668889 None:intergenic 20.0%
!! TGATAATATACAAACACTTA+TGG - chr5_3:66668949-66668968 None:intergenic 20.0%
!!! TTCAATCTGAATTGAATTTT+TGG + chr5_3:66669027-66669046 Msa0826330:intron 20.0%
!!! TGAATTTTTGGTCAATTTAA+AGG + chr5_3:66669039-66669058 Msa0826330:intron 20.0%
!!! AGTTATTATGTATTTGGAAT+AGG - chr5_3:66669439-66669458 None:intergenic 20.0%
!! AATTTCTTCTCTTATATAAG+AGG - chr5_3:66669590-66669609 None:intergenic 20.0%
!!! TATTGCTAGTATTTATTTTC+AGG + chr5_3:66668500-66668519 Msa0826330:intron 20.0%
!! AAGTGAATTCAACAAAAAAT+GGG + chr5_3:66668802-66668821 Msa0826330:CDS 20.0%
!! AACAACATATTAATTACCAT+TGG - chr5_3:66668870-66668889 None:intergenic 20.0%
!! TGATAATATACAAACACTTA+TGG - chr5_3:66668949-66668968 None:intergenic 20.0%
!!! TTCAATCTGAATTGAATTTT+TGG + chr5_3:66669027-66669046 Msa0826330:intron 20.0%
!!! TGAATTTTTGGTCAATTTAA+AGG + chr5_3:66669039-66669058 Msa0826330:intron 20.0%
!!! AGTTATTATGTATTTGGAAT+AGG - chr5_3:66669439-66669458 None:intergenic 20.0%
!! AATTTCTTCTCTTATATAAG+AGG - chr5_3:66669590-66669609 None:intergenic 20.0%
! TAAGTTTCTCTTACAAAAGT+AGG + chr5_3:66668134-66668153 Msa0826330:five_prime_UTR 25.0%
!! TATATATCTTCAGGATCTTT+TGG - chr5_3:66668546-66668565 None:intergenic 25.0%
! GTGAATTATATGAACAGAAT+TGG - chr5_3:66668692-66668711 None:intergenic 25.0%
! ATTCTGTTCATATAATTCAC+AGG + chr5_3:66668692-66668711 Msa0826330:intron 25.0%
! CAAGTGAATTCAACAAAAAA+TGG + chr5_3:66668801-66668820 Msa0826330:CDS 25.0%
!! TTTTGGTCAATTTAAAGGTT+TGG + chr5_3:66669044-66669063 Msa0826330:intron 25.0%
!! ACATGTTGACATATAGTTTT+TGG - chr5_3:66669249-66669268 None:intergenic 25.0%
! AATAAAACAAGCAGAAAATC+AGG - chr5_3:66669297-66669316 None:intergenic 25.0%
! TACATTGTATTTCATCCATA+TGG + chr5_3:66669662-66669681 Msa0826330:three_prime_UTR 25.0%
! TAAGTTTCTCTTACAAAAGT+AGG + chr5_3:66668134-66668153 Msa0826330:five_prime_UTR 25.0%
!! TATATATCTTCAGGATCTTT+TGG - chr5_3:66668546-66668565 None:intergenic 25.0%
! GTGAATTATATGAACAGAAT+TGG - chr5_3:66668692-66668711 None:intergenic 25.0%
! ATTCTGTTCATATAATTCAC+AGG + chr5_3:66668692-66668711 Msa0826330:intron 25.0%
! CAAGTGAATTCAACAAAAAA+TGG + chr5_3:66668801-66668820 Msa0826330:CDS 25.0%
!! TTTTGGTCAATTTAAAGGTT+TGG + chr5_3:66669044-66669063 Msa0826330:intron 25.0%
!! ACATGTTGACATATAGTTTT+TGG - chr5_3:66669249-66669268 None:intergenic 25.0%
! AATAAAACAAGCAGAAAATC+AGG - chr5_3:66669297-66669316 None:intergenic 25.0%
! TACATTGTATTTCATCCATA+TGG + chr5_3:66669662-66669681 Msa0826330:three_prime_UTR 25.0%
TTCTCTTACAAAAGTAGGAT+TGG + chr5_3:66668139-66668158 Msa0826330:five_prime_UTR 30.0%
AGTTGTATCAAAGACAAGAA+TGG + chr5_3:66668205-66668224 Msa0826330:CDS 30.0%
TGCTTACTCCCTTAATATTT+GGG + chr5_3:66668307-66668326 Msa0826330:CDS 30.0%
CTGCAAATCCCAAATATTAA+GGG - chr5_3:66668318-66668337 None:intergenic 30.0%
AATAGGATTGAAACCTTTGA+AGG - chr5_3:66668371-66668390 None:intergenic 30.0%
TAACGAAAAACCGATAATGT+AGG - chr5_3:66668403-66668422 None:intergenic 30.0%
AGATCCTGAAGATATATATG+CGG + chr5_3:66668548-66668567 Msa0826330:CDS 30.0%
ACATAACTCTATACACAGAT+AGG - chr5_3:66668921-66668940 None:intergenic 30.0%
ATTGAACTGTTTGAGAATCA+TGG + chr5_3:66669167-66669186 Msa0826330:intron 30.0%
TATGTCAACATGTAATTACG+AGG + chr5_3:66669256-66669275 Msa0826330:intron 30.0%
ATCACTTTCCTCGATTTATT+TGG - chr5_3:66669357-66669376 None:intergenic 30.0%
TGTAGCAAACACTTAACATA+TGG + chr5_3:66669478-66669497 Msa0826330:three_prime_UTR 30.0%
ATTCTGGATCTGAACTAATA+TGG - chr5_3:66669620-66669639 None:intergenic 30.0%
TTCTCTTACAAAAGTAGGAT+TGG + chr5_3:66668139-66668158 Msa0826330:five_prime_UTR 30.0%
AGTTGTATCAAAGACAAGAA+TGG + chr5_3:66668205-66668224 Msa0826330:CDS 30.0%
TGCTTACTCCCTTAATATTT+GGG + chr5_3:66668307-66668326 Msa0826330:CDS 30.0%
CTGCAAATCCCAAATATTAA+GGG - chr5_3:66668318-66668337 None:intergenic 30.0%
AATAGGATTGAAACCTTTGA+AGG - chr5_3:66668371-66668390 None:intergenic 30.0%
TAACGAAAAACCGATAATGT+AGG - chr5_3:66668403-66668422 None:intergenic 30.0%
AGATCCTGAAGATATATATG+CGG + chr5_3:66668548-66668567 Msa0826330:CDS 30.0%
ACATAACTCTATACACAGAT+AGG - chr5_3:66668921-66668940 None:intergenic 30.0%
ATTGAACTGTTTGAGAATCA+TGG + chr5_3:66669167-66669186 Msa0826330:intron 30.0%
TATGTCAACATGTAATTACG+AGG + chr5_3:66669256-66669275 Msa0826330:intron 30.0%
ATCACTTTCCTCGATTTATT+TGG - chr5_3:66669357-66669376 None:intergenic 30.0%
TGTAGCAAACACTTAACATA+TGG + chr5_3:66669478-66669497 Msa0826330:three_prime_UTR 30.0%
ATTCTGGATCTGAACTAATA+TGG - chr5_3:66669620-66669639 None:intergenic 30.0%
TCTCTTGAGTGACATAGAAA+TGG + chr5_3:66668178-66668197 Msa0826330:exon 35.0%
TCAAAGACAAGAATGGTAAG+AGG + chr5_3:66668212-66668231 Msa0826330:CDS 35.0%
CTGCTTACTCCCTTAATATT+TGG + chr5_3:66668306-66668325 Msa0826330:CDS 35.0%
GCTGCAAATCCCAAATATTA+AGG - chr5_3:66668319-66668338 None:intergenic 35.0%
AATGTAGGAGAGGAATGAAT+AGG - chr5_3:66668388-66668407 None:intergenic 35.0%
TCATTCCTCTCCTACATTAT+CGG + chr5_3:66668390-66668409 Msa0826330:intron 35.0%
AAAAACCGATAATGTAGGAG+AGG - chr5_3:66668398-66668417 None:intergenic 35.0%
AATAAAGTCCAAATCTCACC+TGG - chr5_3:66668603-66668622 None:intergenic 35.0%
TAACTCGTGAACTTTCTGAT+TGG + chr5_3:66668625-66668644 Msa0826330:intron 35.0%
TGGGAAACAGATTTGAGAAT+CGG + chr5_3:66668821-66668840 Msa0826330:CDS 35.0%
CTGAAATGTTTGAGAAACCA+GGG + chr5_3:66669096-66669115 Msa0826330:CDS 35.0%
TAGATGATCATGACATTCTC+TGG + chr5_3:66669123-66669142 Msa0826330:exon 35.0%
AATCATGGCATGAGATATAG+AGG + chr5_3:66669182-66669201 Msa0826330:intron 35.0%
! TCCATCTCTAATTTTCTCTG+AGG - chr5_3:66669208-66669227 None:intergenic 35.0%
TAAATCGAGGAAAGTGATCT+TGG + chr5_3:66669359-66669378 Msa0826330:three_prime_UTR 35.0%
TCAGATCCAGAATCTAAATC+TGG + chr5_3:66669627-66669646 Msa0826330:three_prime_UTR 35.0%
CAACTACCAGATTTAGATTC+TGG - chr5_3:66669636-66669655 None:intergenic 35.0%
AGGACATGTTTATCACCATA+TGG - chr5_3:66669680-66669699 None:intergenic 35.0%
ATGGTGATAAACATGTCCTA+AGG + chr5_3:66669681-66669700 Msa0826330:three_prime_UTR 35.0%
ATAAACATGTCCTAAGGAAC+TGG + chr5_3:66669687-66669706 Msa0826330:three_prime_UTR 35.0%
TCTCTTGAGTGACATAGAAA+TGG + chr5_3:66668178-66668197 Msa0826330:exon 35.0%
TCAAAGACAAGAATGGTAAG+AGG + chr5_3:66668212-66668231 Msa0826330:CDS 35.0%
CTGCTTACTCCCTTAATATT+TGG + chr5_3:66668306-66668325 Msa0826330:CDS 35.0%
GCTGCAAATCCCAAATATTA+AGG - chr5_3:66668319-66668338 None:intergenic 35.0%
AATGTAGGAGAGGAATGAAT+AGG - chr5_3:66668388-66668407 None:intergenic 35.0%
TCATTCCTCTCCTACATTAT+CGG + chr5_3:66668390-66668409 Msa0826330:intron 35.0%
AAAAACCGATAATGTAGGAG+AGG - chr5_3:66668398-66668417 None:intergenic 35.0%
AATAAAGTCCAAATCTCACC+TGG - chr5_3:66668603-66668622 None:intergenic 35.0%
TAACTCGTGAACTTTCTGAT+TGG + chr5_3:66668625-66668644 Msa0826330:intron 35.0%
TGGGAAACAGATTTGAGAAT+CGG + chr5_3:66668821-66668840 Msa0826330:CDS 35.0%
CTGAAATGTTTGAGAAACCA+GGG + chr5_3:66669096-66669115 Msa0826330:CDS 35.0%
TAGATGATCATGACATTCTC+TGG + chr5_3:66669123-66669142 Msa0826330:exon 35.0%
AATCATGGCATGAGATATAG+AGG + chr5_3:66669182-66669201 Msa0826330:intron 35.0%
! TCCATCTCTAATTTTCTCTG+AGG - chr5_3:66669208-66669227 None:intergenic 35.0%
TAAATCGAGGAAAGTGATCT+TGG + chr5_3:66669359-66669378 Msa0826330:three_prime_UTR 35.0%
TCAGATCCAGAATCTAAATC+TGG + chr5_3:66669627-66669646 Msa0826330:three_prime_UTR 35.0%
CAACTACCAGATTTAGATTC+TGG - chr5_3:66669636-66669655 None:intergenic 35.0%
AGGACATGTTTATCACCATA+TGG - chr5_3:66669680-66669699 None:intergenic 35.0%
ATGGTGATAAACATGTCCTA+AGG + chr5_3:66669681-66669700 Msa0826330:three_prime_UTR 35.0%
ATAAACATGTCCTAAGGAAC+TGG + chr5_3:66669687-66669706 Msa0826330:three_prime_UTR 35.0%
TTGGGATTTGCAGCATTTCA+TGG + chr5_3:66668325-66668344 Msa0826330:CDS 40.0%
! GATTGAAACCTTTGAAGGTG+AGG - chr5_3:66668366-66668385 None:intergenic 40.0%
AGTGCCGCATATATATCTTC+AGG - chr5_3:66668555-66668574 None:intergenic 40.0%
GATTGTCGCGATTATACCAA+TGG + chr5_3:66668851-66668870 Msa0826330:CDS 40.0%
! GGTTTGGTCAGACAACTTAT+TGG + chr5_3:66669060-66669079 Msa0826330:intron 40.0%
GCTGAAATGTTTGAGAAACC+AGG + chr5_3:66669095-66669114 Msa0826330:CDS 40.0%
TCATGATCATCTAGTTACCC+TGG - chr5_3:66669116-66669135 None:intergenic 40.0%
TCATGACATTCTCTGGATCA+TGG + chr5_3:66669130-66669149 Msa0826330:three_prime_UTR 40.0%
GCCTCAGAGAAAATTAGAGA+TGG + chr5_3:66669204-66669223 Msa0826330:intron 40.0%
!! CCTCGATTTATTTGGCTTTG+AGG - chr5_3:66669349-66669368 None:intergenic 40.0%
CCTCAAAGCCAAATAAATCG+AGG + chr5_3:66669346-66669365 Msa0826330:three_prime_UTR 40.0%
TTGGGATTTGCAGCATTTCA+TGG + chr5_3:66668325-66668344 Msa0826330:CDS 40.0%
! GATTGAAACCTTTGAAGGTG+AGG - chr5_3:66668366-66668385 None:intergenic 40.0%
AGTGCCGCATATATATCTTC+AGG - chr5_3:66668555-66668574 None:intergenic 40.0%
GATTGTCGCGATTATACCAA+TGG + chr5_3:66668851-66668870 Msa0826330:CDS 40.0%
! GGTTTGGTCAGACAACTTAT+TGG + chr5_3:66669060-66669079 Msa0826330:intron 40.0%
GCTGAAATGTTTGAGAAACC+AGG + chr5_3:66669095-66669114 Msa0826330:CDS 40.0%
TCATGATCATCTAGTTACCC+TGG - chr5_3:66669116-66669135 None:intergenic 40.0%
TCATGACATTCTCTGGATCA+TGG + chr5_3:66669130-66669149 Msa0826330:three_prime_UTR 40.0%
GCCTCAGAGAAAATTAGAGA+TGG + chr5_3:66669204-66669223 Msa0826330:intron 40.0%
!! CCTCGATTTATTTGGCTTTG+AGG - chr5_3:66669349-66669368 None:intergenic 40.0%
CCTCAAAGCCAAATAAATCG+AGG + chr5_3:66669346-66669365 Msa0826330:three_prime_UTR 40.0%
AAGAATGGTAAGAGGCAGAG+AGG + chr5_3:66668220-66668239 Msa0826330:CDS 45.0%
! AATGCCTCTAAGGGCTTCAA+AGG + chr5_3:66668259-66668278 Msa0826330:CDS 45.0%
! ATGCCTCTAAGGGCTTCAAA+GGG + chr5_3:66668260-66668279 Msa0826330:CDS 45.0%
GCAGACATAAGCAATTGTGG+TGG - chr5_3:66668287-66668306 None:intergenic 45.0%
GCAGCAGACATAAGCAATTG+TGG - chr5_3:66668290-66668309 None:intergenic 45.0%
CAAACCTTCCTCACCTTCAA+AGG + chr5_3:66668355-66668374 Msa0826330:CDS 45.0%
! GAAACCTTTGAAGGTGAGGA+AGG - chr5_3:66668362-66668381 None:intergenic 45.0%
TTAGACGCTCTCTAAGCTTG+AGG - chr5_3:66668472-66668491 None:intergenic 45.0%
ACTAGCCAACATTTGCTTCG+CGG - chr5_3:66668749-66668768 None:intergenic 45.0%
! CGAAGCAAATGTTGGCTAGT+TGG + chr5_3:66668749-66668768 Msa0826330:CDS 45.0%
ATATGCAGAAGCAGATGCTG+TGG + chr5_3:66668772-66668791 Msa0826330:CDS 45.0%
GAGGAAAGTGATCTTGGACA+TGG + chr5_3:66669365-66669384 Msa0826330:three_prime_UTR 45.0%
GAACAACACTCCAGTTCCTT+AGG - chr5_3:66669700-66669719 None:intergenic 45.0%
AAGAATGGTAAGAGGCAGAG+AGG + chr5_3:66668220-66668239 Msa0826330:CDS 45.0%
! AATGCCTCTAAGGGCTTCAA+AGG + chr5_3:66668259-66668278 Msa0826330:CDS 45.0%
! ATGCCTCTAAGGGCTTCAAA+GGG + chr5_3:66668260-66668279 Msa0826330:CDS 45.0%
GCAGACATAAGCAATTGTGG+TGG - chr5_3:66668287-66668306 None:intergenic 45.0%
GCAGCAGACATAAGCAATTG+TGG - chr5_3:66668290-66668309 None:intergenic 45.0%
CAAACCTTCCTCACCTTCAA+AGG + chr5_3:66668355-66668374 Msa0826330:CDS 45.0%
! GAAACCTTTGAAGGTGAGGA+AGG - chr5_3:66668362-66668381 None:intergenic 45.0%
TTAGACGCTCTCTAAGCTTG+AGG - chr5_3:66668472-66668491 None:intergenic 45.0%
ACTAGCCAACATTTGCTTCG+CGG - chr5_3:66668749-66668768 None:intergenic 45.0%
! CGAAGCAAATGTTGGCTAGT+TGG + chr5_3:66668749-66668768 Msa0826330:CDS 45.0%
ATATGCAGAAGCAGATGCTG+TGG + chr5_3:66668772-66668791 Msa0826330:CDS 45.0%
GAGGAAAGTGATCTTGGACA+TGG + chr5_3:66669365-66669384 Msa0826330:three_prime_UTR 45.0%
GAACAACACTCCAGTTCCTT+AGG - chr5_3:66669700-66669719 None:intergenic 45.0%
! AGGCAGAGAGGAAGTGTATG+TGG + chr5_3:66668232-66668251 Msa0826330:CDS 50.0%
ATGTGGCAGCAATGCCTCTA+AGG + chr5_3:66668249-66668268 Msa0826330:CDS 50.0%
TGTGGCAGCAATGCCTCTAA+GGG + chr5_3:66668250-66668269 Msa0826330:CDS 50.0%
GTCCGAATGTTCACCATCGA+CGG + chr5_3:66668445-66668464 Msa0826330:intron 50.0%
GTCCGTCGATGGTGAACATT+CGG - chr5_3:66668450-66668469 None:intergenic 50.0%
!! GCGGCACTAGCAGTTTTATC+TGG + chr5_3:66668567-66668586 Msa0826330:CDS 50.0%
TTATCTGGTAGAGCTGTGCC+AGG + chr5_3:66668582-66668601 Msa0826330:CDS 50.0%
TCACAGGAGCTGTTCTTGTG+AGG + chr5_3:66668708-66668727 Msa0826330:intron 50.0%
GATTGCCGCGAAGCAAATGT+TGG + chr5_3:66668741-66668760 Msa0826330:CDS 50.0%
! AGGCAGAGAGGAAGTGTATG+TGG + chr5_3:66668232-66668251 Msa0826330:CDS 50.0%
ATGTGGCAGCAATGCCTCTA+AGG + chr5_3:66668249-66668268 Msa0826330:CDS 50.0%
TGTGGCAGCAATGCCTCTAA+GGG + chr5_3:66668250-66668269 Msa0826330:CDS 50.0%
GTCCGAATGTTCACCATCGA+CGG + chr5_3:66668445-66668464 Msa0826330:intron 50.0%
GTCCGTCGATGGTGAACATT+CGG - chr5_3:66668450-66668469 None:intergenic 50.0%
!! GCGGCACTAGCAGTTTTATC+TGG + chr5_3:66668567-66668586 Msa0826330:CDS 50.0%
TTATCTGGTAGAGCTGTGCC+AGG + chr5_3:66668582-66668601 Msa0826330:CDS 50.0%
TCACAGGAGCTGTTCTTGTG+AGG + chr5_3:66668708-66668727 Msa0826330:intron 50.0%
GATTGCCGCGAAGCAAATGT+TGG + chr5_3:66668741-66668760 Msa0826330:CDS 50.0%
!! GGTCCCTTTGAAGCCCTTAG+AGG - chr5_3:66668266-66668285 None:intergenic 55.0%
CTAAGCTTGAGGTCCGTCGA+TGG - chr5_3:66668461-66668480 None:intergenic 55.0%
GAGCTGTGCCAGGTGAGATT+TGG + chr5_3:66668592-66668611 Msa0826330:intron 55.0%
!! GGTCCCTTTGAAGCCCTTAG+AGG - chr5_3:66668266-66668285 None:intergenic 55.0%
CTAAGCTTGAGGTCCGTCGA+TGG - chr5_3:66668461-66668480 None:intergenic 55.0%
GAGCTGTGCCAGGTGAGATT+TGG + chr5_3:66668592-66668611 Msa0826330:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 66668133 66669734 66668133 ID=Msa0826330;Name=Msa0826330
chr5_3 mRNA 66668133 66669734 66668133 ID=Msa0826330-mRNA-1;Parent=Msa0826330;Name=Msa0826330-mRNA-1;_AED=0.15;_eAED=0.15;_QI=64|1|1|1|0.75|0.8|5|435|161
chr5_3 exon 66668133 66668376 66668133 ID=Msa0826330-mRNA-1:exon:20911;Parent=Msa0826330-mRNA-1
chr5_3 exon 66668522 66668603 66668522 ID=Msa0826330-mRNA-1:exon:20912;Parent=Msa0826330-mRNA-1
chr5_3 exon 66668714 66668872 66668714 ID=Msa0826330-mRNA-1:exon:20913;Parent=Msa0826330-mRNA-1
chr5_3 exon 66669061 66669151 66669061 ID=Msa0826330-mRNA-1:exon:20914;Parent=Msa0826330-mRNA-1
chr5_3 exon 66669326 66669734 66669326 ID=Msa0826330-mRNA-1:exon:20915;Parent=Msa0826330-mRNA-1
chr5_3 five_prime_UTR 66668133 66668196 66668133 ID=Msa0826330-mRNA-1:five_prime_utr;Parent=Msa0826330-mRNA-1
chr5_3 CDS 66668197 66668376 66668197 ID=Msa0826330-mRNA-1:cds;Parent=Msa0826330-mRNA-1
chr5_3 CDS 66668522 66668603 66668522 ID=Msa0826330-mRNA-1:cds;Parent=Msa0826330-mRNA-1
chr5_3 CDS 66668714 66668872 66668714 ID=Msa0826330-mRNA-1:cds;Parent=Msa0826330-mRNA-1
chr5_3 CDS 66669061 66669125 66669061 ID=Msa0826330-mRNA-1:cds;Parent=Msa0826330-mRNA-1
chr5_3 three_prime_UTR 66669126 66669151 66669126 ID=Msa0826330-mRNA-1:three_prime_utr;Parent=Msa0826330-mRNA-1
chr5_3 three_prime_UTR 66669326 66669734 66669326 ID=Msa0826330-mRNA-1:three_prime_utr;Parent=Msa0826330-mRNA-1
Gene Sequence

>Msa0826330

ATGGAATCAGTTGTATCAAAGACAAGAATGGTAAGAGGCAGAGAGGAAGTGTATGTGGCAGCAATGCCTCTAAGGGCTTCAAAGGGACCACCACAATTGCTTATGTCTGCTGCTTACTCCCTTAATATTTGGGATTTGCAGCATTTCATGGTCATCATCAAACCTTCCTCACCTTCAAAGGTTCTAGTTTTTGATTTTCAACCAAAAGATCCTGAAGATATATATGCGGCACTAGCAGTTTTATCTGGTAGAGCTGTGCCAGGAGCTGTTCTTGTGAGGAAGTTAAAAAGATTGCCGCGAAGCAAATGTTGGCTAGTTGGATATGCAGAAGCAGATGCTGTGGAAATAGCAAGTGAATTCAACAAAAAATGGGAAACAGATTTGAGAATCGGTGTTAATGATTGTCGCGATTATACCAATGGTTTGGTCAGACAACTTATTGGCGAAGAAGATGTGCTGAAATGTTTGAGAAACCAGGGTAACTAG
Protein sequence

>Msa0826330

MESVVSKTRMVRGREEVYVAAMPLRASKGPPQLLMSAAYSLNIWDLQHFMVIIKPSSPSKVLVFDFQPKDPEDIYAALAVLSGRAVPGAVLVRKLKRLPRSKCWLVGYAEADAVEIASEFNKKWETDLRIGVNDCRDYTNGLVRQLIGEEDVLKCLRNQGN*