AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0771290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0771290 MtrunA17_Chr5g0425231 95.946 74 3 0 1 74 1 74 3.49e-48 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0771290 AT1G22140.1 61.111 72 26 1 1 72 1 70 4.59e-26 91.3
Msa0771290 AT1G22140.3 61.111 72 26 1 1 72 1 70 4.59e-26 91.3
Msa0771290 AT1G22140.2 51.923 52 23 1 1 52 1 50 8.42e-11 52.4

Find 31 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATTATGTTTGTGCCTT+TGG 0.309264 5_2:-43619070 Msa0771290:three_prime_UTR
GGGCCTTTGTAGTTGGGTGG+TGG 0.311833 5_2:-43621618 Msa0771290:five_prime_UTR
AATATCCTCTGCCCGTTTCC+TGG 0.320075 5_2:+43619286 None:intergenic
ACAGCTCTCGGCCGTTCATT+TGG 0.346748 5_2:+43619249 None:intergenic
ATGCAAGTAAAAGTTGAATA+AGG 0.354751 5_2:+43618895 None:intergenic
GGCGCTTCCATGAGAGGAAT+TGG 0.399752 5_2:+43621519 None:intergenic
TGTGTGAGAGAAGCTCTTAT+TGG 0.421337 5_2:+43621545 None:intergenic
GGCCTTTGTAGTTGGGTGGT+GGG 0.434971 5_2:-43621617 Msa0771290:five_prime_UTR
CTTTGTGAATTATGATCAGT+TGG 0.440941 5_2:-43619005 Msa0771290:three_prime_UTR
TATTTATAGAAATATACTCT+TGG 0.447147 5_2:-43619093 Msa0771290:three_prime_UTR
AGCTGAACAATCCAGGAAAC+GGG 0.459746 5_2:-43619297 Msa0771290:CDS
AAGCTGAACAATCCAGGAAA+CGG 0.483722 5_2:-43619298 Msa0771290:CDS
TCAGAGAAGAAATTCTTTGT+CGG 0.484141 5_2:+43618963 None:intergenic
CTCTCGGCCGTTCATTTGGA+CGG 0.490547 5_2:+43619253 None:intergenic
CAACACAGTTTCTTTGCCAA+AGG 0.501740 5_2:+43619054 None:intergenic
CCAGAAAAGCTGAACAATCC+AGG 0.512185 5_2:-43619304 Msa0771290:CDS
CACAAGACCAATTCCTCTCA+TGG 0.513419 5_2:-43621526 Msa0771290:CDS
AGCTGTTTCTGCATGAGCCA+TGG 0.520951 5_2:-43619232 Msa0771290:CDS
GCTGTTTCTGCATGAGCCAT+GGG 0.535201 5_2:-43619231 Msa0771290:CDS
CGGGCAGAGGATATTGCCGC+TGG 0.540135 5_2:-43619278 Msa0771290:CDS
CCTTGTGGCGCTTCCATGAG+AGG 0.546025 5_2:+43621513 None:intergenic
GGTGGGAAACGAGAACGCGA+TGG 0.555499 5_2:-43621600 Msa0771290:exon
GTGGGAAACGAGAACGCGAT+GGG 0.571946 5_2:-43621599 Msa0771290:exon
GTTCATTTGGACGGTTCCAG+CGG 0.581449 5_2:+43619262 None:intergenic
ACAATCCAGGAAACGGGCAG+AGG 0.600748 5_2:-43619291 Msa0771290:CDS
GCTGGAACCGTCCAAATGAA+CGG 0.602726 5_2:-43619260 Msa0771290:CDS
CCTCTCATGGAAGCGCCACA+AGG 0.617168 5_2:-43621513 Msa0771290:intron
TTCCCACCACCCAACTACAA+AGG 0.632686 5_2:+43621615 None:intergenic
CGTGAGTCATCAAACACCCA+TGG 0.651485 5_2:+43619215 None:intergenic
GCTCATGCAGAAACAGCTCT+CGG 0.665779 5_2:+43619237 None:intergenic
CTTTGGCAAAGAAACTGTGT+TGG 0.669822 5_2:-43619053 Msa0771290:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTAAAATATGTTAAAA+AGG + chr5_2:43620377-43620396 None:intergenic 10.0%
!!! TTTTAACATATTTTAAAATC+AGG - chr5_2:43620377-43620396 Msa0771290:intron 10.0%
!!! TTTAACATATTTTAAAATCA+GGG - chr5_2:43620378-43620397 Msa0771290:intron 10.0%
!! ATAAATTAAAATGTTAAAGT+AGG + chr5_2:43620490-43620509 None:intergenic 10.0%
!! TAAGATTTAGTAAAATGATA+TGG + chr5_2:43619067-43619086 None:intergenic 15.0%
!!! CATATCATTTATTTTAAGAT+CGG - chr5_2:43619631-43619650 Msa0771290:intron 15.0%
!! TATAAACTAACTACTTTAAA+GGG + chr5_2:43620043-43620062 None:intergenic 15.0%
!! TTTATCATTGAGTAATTAAA+TGG - chr5_2:43620520-43620539 Msa0771290:intron 15.0%
!! TATTTATAGAAATATACTCT+TGG - chr5_2:43621411-43621430 Msa0771290:intron 15.0%
!! TAAAAAAAAAAACGACACAT+TGG - chr5_2:43618859-43618878 Msa0771290:three_prime_UTR 20.0%
!!! TATATATATGCTATTTCTAC+AGG + chr5_2:43619322-43619341 None:intergenic 20.0%
!! TCTTGTAATTGAATCTTAAA+AGG + chr5_2:43619837-43619856 None:intergenic 20.0%
!! TTAAGATTCAATTACAAGAA+TGG - chr5_2:43619838-43619857 Msa0771290:intron 20.0%
!!! ATAAACTAACTACTTTAAAG+GGG + chr5_2:43620042-43620061 None:intergenic 20.0%
!! GTATAAACTAACTACTTTAA+AGG + chr5_2:43620044-43620063 None:intergenic 20.0%
!!! AAAGTAGTTAGTTTATACAA+CGG - chr5_2:43620046-43620065 Msa0771290:intron 20.0%
!!! ACTTTTAAAAATTTTGTCTG+TGG + chr5_2:43620130-43620149 None:intergenic 20.0%
!! AATATGTTAAAAAGGATCTA+AGG + chr5_2:43620369-43620388 None:intergenic 20.0%
!! AATGAATCCATCTATATATT+TGG + chr5_2:43620450-43620469 None:intergenic 20.0%
!! TTCTCAAAAAATCATCTTAA+AGG + chr5_2:43621090-43621109 None:intergenic 20.0%
!!! TTTTAGAGAAAGGATTAAAT+AGG + chr5_2:43621137-43621156 None:intergenic 20.0%
!!! AATTGCTTCCGTTTTATTAT+AGG - chr5_2:43619036-43619055 Msa0771290:three_prime_UTR 25.0%
! TGGCATATCCTATAATAAAA+CGG + chr5_2:43619047-43619066 None:intergenic 25.0%
!!! GTTGTTTTAATAAGCTATCT+AGG + chr5_2:43619257-43619276 None:intergenic 25.0%
! TCTTGTAACTGAATCTTAAA+AGG + chr5_2:43619948-43619967 None:intergenic 25.0%
! ACAATGAATAAACATGTTAG+TGG - chr5_2:43620155-43620174 Msa0771290:intron 25.0%
!!! TTGTTTTGAACTTTTGGTTA+TGG - chr5_2:43620338-43620357 Msa0771290:intron 25.0%
! CTAAAATACCTATCATTCAA+CGG + chr5_2:43620704-43620723 None:intergenic 25.0%
!! GTTGAATGATAGGTATTTTA+GGG - chr5_2:43620703-43620722 Msa0771290:intron 25.0%
! AGTATTTATAGAGTAGGTTT+AGG - chr5_2:43620762-43620781 Msa0771290:intron 25.0%
! GTATTTATAGAGTAGGTTTA+GGG - chr5_2:43620763-43620782 Msa0771290:intron 25.0%
! TTTCTCATAAAACCTATACT+TGG - chr5_2:43620788-43620807 Msa0771290:intron 25.0%
! AAATATTATGTTTGTGCCTT+TGG - chr5_2:43621434-43621453 Msa0771290:intron 25.0%
! ATGCAAGTAAAAGTTGAATA+AGG + chr5_2:43621612-43621631 None:intergenic 25.0%
!!! AGTTTGATCTGTTTCTTTTT+AGG - chr5_2:43621644-43621663 Msa0771290:five_prime_UTR 25.0%
AAAAAAAACGACACATTGGT+TGG - chr5_2:43618863-43618882 Msa0771290:three_prime_UTR 30.0%
! AAAAAAACGACACATTGGTT+GGG - chr5_2:43618864-43618883 Msa0771290:three_prime_UTR 30.0%
CAAAAATCATGATTGTTACC+AGG - chr5_2:43619136-43619155 Msa0771290:three_prime_UTR 30.0%
AATAAGCTGCTTATCCAAAT+AGG - chr5_2:43619393-43619412 Msa0771290:intron 30.0%
ATAAGCTGCTTATCCAAATA+GGG - chr5_2:43619394-43619413 Msa0771290:intron 30.0%
TCAAACTTCAAACCTTAAAC+AGG - chr5_2:43619473-43619492 Msa0771290:intron 30.0%
TCAAACCTTAAACAGGAAAT+TGG - chr5_2:43619480-43619499 Msa0771290:intron 30.0%
! AGTGTTGAAACATGAATACA+AGG + chr5_2:43619513-43619532 None:intergenic 30.0%
TTCAACACTTAGATGCAATT+TGG - chr5_2:43619524-43619543 Msa0771290:intron 30.0%
TCAACACTTAGATGCAATTT+GGG - chr5_2:43619525-43619544 Msa0771290:intron 30.0%
! GTGTTTTCATTGACGTATAT+TGG + chr5_2:43619672-43619691 None:intergenic 30.0%
CGTCAAATTCAACTGAAAAT+TGG + chr5_2:43619724-43619743 None:intergenic 30.0%
TCTCATAAAATGCACATGTA+AGG - chr5_2:43619884-43619903 Msa0771290:intron 30.0%
ACATGTAAGGTTATTGTTGA+AGG - chr5_2:43619897-43619916 Msa0771290:intron 30.0%
GAACTGACATTTCAAAATAG+TGG - chr5_2:43619970-43619989 Msa0771290:intron 30.0%
!! GAATATTTTTGCTCATAGCA+TGG + chr5_2:43620269-43620288 None:intergenic 30.0%
!!! CTGGATTTGTTTTGAACTTT+TGG - chr5_2:43620332-43620351 Msa0771290:intron 30.0%
CAACAGACCAAATATATAGA+TGG - chr5_2:43620440-43620459 Msa0771290:intron 30.0%
ATCACTAGTAATAACTGTGT+AGG + chr5_2:43620546-43620565 None:intergenic 30.0%
! CGTTGAATGATAGGTATTTT+AGG - chr5_2:43620702-43620721 Msa0771290:intron 30.0%
ACTCTATAAATACTAACCCA+TGG + chr5_2:43620756-43620775 None:intergenic 30.0%
!! TGGGTTAGTATTTATAGAGT+AGG - chr5_2:43620756-43620775 Msa0771290:intron 30.0%
TTGTGACCAATTTAATGAGA+TGG - chr5_2:43621037-43621056 Msa0771290:intron 30.0%
! AGAAGTTTTTGTCTATTGCA+AGG - chr5_2:43621106-43621125 Msa0771290:intron 30.0%
! GAAGTTTTTGTCTATTGCAA+GGG - chr5_2:43621107-43621126 Msa0771290:intron 30.0%
!! GATAGTAGCATTTTAGAGAA+AGG + chr5_2:43621147-43621166 None:intergenic 30.0%
!!! TTTTTGAGGTTTCTTTTCGT+TGG - chr5_2:43621312-43621331 Msa0771290:intron 30.0%
!!! TTTTGAGGTTTCTTTTCGTT+GGG - chr5_2:43621313-43621332 Msa0771290:intron 30.0%
CTTTGTGAATTATGATCAGT+TGG - chr5_2:43621499-43621518 Msa0771290:intron 30.0%
TCAGAGAAGAAATTCTTTGT+CGG + chr5_2:43621544-43621563 None:intergenic 30.0%
! AAAAAACGACACATTGGTTG+GGG - chr5_2:43618865-43618884 Msa0771290:three_prime_UTR 35.0%
GTTCAAAATTAGCGCATGTT+TGG - chr5_2:43619158-43619177 Msa0771290:three_prime_UTR 35.0%
GCTAACCAATTTCCTGTTTA+AGG + chr5_2:43619488-43619507 None:intergenic 35.0%
TTGAATTTGACGCAACACAT+GGG - chr5_2:43619732-43619751 Msa0771290:intron 35.0%
GGACTGACATTTCAAAATAG+TGG - chr5_2:43619859-43619878 Msa0771290:intron 35.0%
TTCTCATAAAACGCACTTGT+AGG - chr5_2:43619994-43620013 Msa0771290:intron 35.0%
! ACTAACTACTTTAAAGGGGA+TGG + chr5_2:43620038-43620057 None:intergenic 35.0%
TCTAGCTATCCATATATTCC+AGG + chr5_2:43620193-43620212 None:intergenic 35.0%
GTTATTACTAGTGATCATGC+CGG - chr5_2:43620551-43620570 Msa0771290:intron 35.0%
AATGAAACAACCCCAATTAG+AGG + chr5_2:43620609-43620628 None:intergenic 35.0%
AAGGACAGTTATCCAAGTAT+AGG + chr5_2:43620803-43620822 None:intergenic 35.0%
AGCCTCTTAGAACATATGTT+GGG - chr5_2:43620969-43620988 Msa0771290:intron 35.0%
! AACCCAACATATGTTCTAAG+AGG + chr5_2:43620974-43620993 None:intergenic 35.0%
TCTTAGAACATATGTTGGGT+TGG - chr5_2:43620973-43620992 Msa0771290:intron 35.0%
CTTAGAACATATGTTGGGTT+GGG - chr5_2:43620974-43620993 Msa0771290:intron 35.0%
AATACTATCCGTAGTTGTTC+CGG + chr5_2:43621012-43621031 None:intergenic 35.0%
CCAAGTCCATCTCATTAAAT+TGG + chr5_2:43621046-43621065 None:intergenic 35.0%
! CCAATTTAATGAGATGGACT+TGG - chr5_2:43621043-43621062 Msa0771290:intron 35.0%
!! TTTGATGACTCACGTTTTTG+AGG - chr5_2:43621298-43621317 Msa0771290:intron 35.0%
! CGTCGATAAAAAGCATACAT+TGG + chr5_2:43621365-43621384 None:intergenic 35.0%
!! ATGTATGCTTTTTATCGACG+TGG - chr5_2:43621365-43621384 Msa0771290:intron 35.0%
!! TGTATGCTTTTTATCGACGT+GGG - chr5_2:43621366-43621385 Msa0771290:intron 35.0%
! AAAAACGACACATTGGTTGG+GGG - chr5_2:43618866-43618885 Msa0771290:three_prime_UTR 40.0%
TGTGTGAGAGAAGCTCTTAT+TGG + chr5_2:43618962-43618981 None:intergenic 40.0%
GACGCAATAAACTAACCTTG+TGG + chr5_2:43619009-43619028 None:intergenic 40.0%
! ACATGCGCTAATTTTGAACC+TGG + chr5_2:43619157-43619176 None:intergenic 40.0%
CACAAACAGAGTAACAACCA+AGG + chr5_2:43619420-43619439 None:intergenic 40.0%
GACTTTAGTAGCATGATCTG+TGG - chr5_2:43619446-43619465 Msa0771290:intron 40.0%
TTTAGTAGCATGATCTGTGG+TGG - chr5_2:43619449-43619468 Msa0771290:intron 40.0%
GTTGAATTTGACGCAACACA+TGG - chr5_2:43619731-43619750 Msa0771290:intron 40.0%
CTTCAACAAATGCGAAGAAC+AGG + chr5_2:43619789-43619808 None:intergenic 40.0%
! CTGTTCTTCGCATTTGTTGA+AGG - chr5_2:43619787-43619806 Msa0771290:intron 40.0%
CCATCAAATCAAGCATTACG+TGG + chr5_2:43620095-43620114 None:intergenic 40.0%
! CCACGTAATGCTTGATTTGA+TGG - chr5_2:43620092-43620111 Msa0771290:intron 40.0%
TATATTCCAGGAAGCCATCA+GGG + chr5_2:43620181-43620200 None:intergenic 40.0%
ATATATTCCAGGAAGCCATC+AGG + chr5_2:43620182-43620201 None:intergenic 40.0%
TGATGGCTTCCTGGAATATA+TGG - chr5_2:43620181-43620200 Msa0771290:intron 40.0%
!! ACAAGCCTTATTCCACTAAG+TGG - chr5_2:43620238-43620257 Msa0771290:intron 40.0%
!! CAAGCCTTATTCCACTAAGT+GGG - chr5_2:43620239-43620258 Msa0771290:intron 40.0%
!! AAGCCTTATTCCACTAAGTG+GGG - chr5_2:43620240-43620259 Msa0771290:intron 40.0%
!! CTAGTAGCTCTGAGATATTC+TGG - chr5_2:43620313-43620332 Msa0771290:intron 40.0%
GCGTTGAATGACCTCTAATT+GGG - chr5_2:43620595-43620614 Msa0771290:intron 40.0%
CGTTGAATGACCTCTAATTG+GGG - chr5_2:43620596-43620615 Msa0771290:intron 40.0%
AATACTAACCCATGGACACT+GGG + chr5_2:43620748-43620767 None:intergenic 40.0%
AAATACTAACCCATGGACAC+TGG + chr5_2:43620749-43620768 None:intergenic 40.0%
TACTTGGATAACTGTCCTTG+AGG - chr5_2:43620804-43620823 Msa0771290:intron 40.0%
ACTTGGATAACTGTCCTTGA+GGG - chr5_2:43620805-43620824 Msa0771290:intron 40.0%
ACAGTTGTGAACAGATCAAG+AGG - chr5_2:43620848-43620867 Msa0771290:intron 40.0%
TGAACAGATCAAGAGGAATC+TGG - chr5_2:43620855-43620874 Msa0771290:intron 40.0%
!! TCATTTTTCCTGTGATGTCC+TGG - chr5_2:43620906-43620925 Msa0771290:intron 40.0%
TCTAAGAGGCTCTTTGTACA+AGG + chr5_2:43620960-43620979 None:intergenic 40.0%
GAGCCTCTTAGAACATATGT+TGG - chr5_2:43620968-43620987 Msa0771290:intron 40.0%
! CTATCTGTATTGCCTTTTGC+AGG - chr5_2:43621162-43621181 Msa0771290:intron 40.0%
AAGCTGAACAATCCAGGAAA+CGG - chr5_2:43621206-43621225 Msa0771290:intron 40.0%
CAACACAGTTTCTTTGCCAA+AGG + chr5_2:43621453-43621472 None:intergenic 40.0%
CTTTGGCAAAGAAACTGTGT+TGG - chr5_2:43621451-43621470 Msa0771290:intron 40.0%
CACAAGACCAATTCCTCTCA+TGG - chr5_2:43618978-43618997 Msa0771290:three_prime_UTR 45.0%
TGCTTATCCAAATAGGGCCT+TGG - chr5_2:43619400-43619419 Msa0771290:intron 45.0%
GTAACAACCAAGGCCCTATT+TGG + chr5_2:43619410-43619429 None:intergenic 45.0%
!! GATGCAATTTGGGTTGAGCT+TGG - chr5_2:43619535-43619554 Msa0771290:intron 45.0%
AATTTGACGCAACACATGGG+AGG - chr5_2:43619735-43619754 Msa0771290:intron 45.0%
AAACATGTTAGTGGCCCTGA+TGG - chr5_2:43620164-43620183 Msa0771290:intron 45.0%
TCAACCCAGGATCTTAGATC+CGG + chr5_2:43620573-43620592 None:intergenic 45.0%
! GATCTAAGATCCTGGGTTGA+TGG - chr5_2:43620573-43620592 Msa0771290:intron 45.0%
! GGCGTTGAATGACCTCTAAT+TGG - chr5_2:43620594-43620613 Msa0771290:intron 45.0%
! CTTTTTGTCCGAGCGAATGA+GGG - chr5_2:43620637-43620656 Msa0771290:intron 45.0%
TCAAGAGGAATCTGGACTGA+AGG - chr5_2:43620863-43620882 Msa0771290:intron 45.0%
CAAGAGGAATCTGGACTGAA+GGG - chr5_2:43620864-43620883 Msa0771290:intron 45.0%
AAGGGTCCAAATTGCTTCCA+GGG - chr5_2:43620882-43620901 Msa0771290:intron 45.0%
AATGACCCCTGGAAGCAATT+TGG + chr5_2:43620891-43620910 None:intergenic 45.0%
ATCACAGGAAAAATGACCCC+TGG + chr5_2:43620902-43620921 None:intergenic 45.0%
GTCCATACCATGGTCCTTAT+GGG - chr5_2:43620934-43620953 Msa0771290:intron 45.0%
CTCTTTGTACAAGGCCCATA+AGG + chr5_2:43620951-43620970 None:intergenic 45.0%
!! ACATATGTTGGGTTGGGTCT+CGG - chr5_2:43620980-43620999 Msa0771290:intron 45.0%
ATCCGTAGTTGTTCCGGTTT+AGG + chr5_2:43621006-43621025 None:intergenic 45.0%
ATTCAGCAGCATCCTGCAAA+AGG + chr5_2:43621177-43621196 None:intergenic 45.0%
! CCTGGATTGTTCAGCTTTTC+TGG + chr5_2:43621203-43621222 None:intergenic 45.0%
CCAGAAAAGCTGAACAATCC+AGG - chr5_2:43621200-43621219 Msa0771290:intron 45.0%
AGCTGAACAATCCAGGAAAC+GGG - chr5_2:43621207-43621226 Msa0771290:intron 45.0%
!! AAAAATTTGATATAATTTAA+CGG + chr5_2:43619096-43619115 None:intergenic 5.0%
TTCCCACCACCCAACTACAA+AGG + chr5_2:43618892-43618911 None:intergenic 50.0%
CGACCCCACTTAGTGGAATA+AGG + chr5_2:43620246-43620265 None:intergenic 50.0%
! TCATGCCGGATCTAAGATCC+TGG - chr5_2:43620565-43620584 Msa0771290:intron 50.0%
! CATGCCGGATCTAAGATCCT+GGG - chr5_2:43620566-43620585 Msa0771290:intron 50.0%
TCATTCAACGCCATCAACCC+AGG + chr5_2:43620586-43620605 None:intergenic 50.0%
CCTCATTCGCTCGGACAAAA+AGG + chr5_2:43620639-43620658 None:intergenic 50.0%
! CCTTTTTGTCCGAGCGAATG+AGG - chr5_2:43620636-43620655 Msa0771290:intron 50.0%
! AGTAGGCGCCGTTGAATGAT+AGG - chr5_2:43620693-43620712 Msa0771290:intron 50.0%
GTACCTAACCCAGTGTCCAT+GGG - chr5_2:43620737-43620756 Msa0771290:intron 50.0%
TAACCCATGGACACTGGGTT+AGG + chr5_2:43620743-43620762 None:intergenic 50.0%
AGGACTACAGTTGTCCCTCA+AGG + chr5_2:43620822-43620841 None:intergenic 50.0%
AGGGACAACTGTAGTCCTTG+AGG - chr5_2:43620824-43620843 Msa0771290:intron 50.0%
GGGACAACTGTAGTCCTTGA+GGG - chr5_2:43620825-43620844 Msa0771290:intron 50.0%
CTGTTCACAACTGTCCCTCA+AGG + chr5_2:43620842-43620861 None:intergenic 50.0%
GAAGGGTCCAAATTGCTTCC+AGG - chr5_2:43620881-43620900 Msa0771290:intron 50.0%
AGGGTCCAAATTGCTTCCAG+GGG - chr5_2:43620883-43620902 Msa0771290:intron 50.0%
TTCCTGTGATGTCCTGGATG+AGG - chr5_2:43620912-43620931 Msa0771290:intron 50.0%
GGTCCATACCATGGTCCTTA+TGG - chr5_2:43620933-43620952 Msa0771290:intron 50.0%
TACAAGGCCCATAAGGACCA+TGG + chr5_2:43620944-43620963 None:intergenic 50.0%
GGCCTAAACCGGAACAACTA+CGG - chr5_2:43621001-43621020 Msa0771290:intron 50.0%
AATATCCTCTGCCCGTTTCC+TGG + chr5_2:43621221-43621240 None:intergenic 50.0%
GTTCATTTGGACGGTTCCAG+CGG + chr5_2:43621245-43621264 None:intergenic 50.0%
GCTGGAACCGTCCAAATGAA+CGG - chr5_2:43621244-43621263 Msa0771290:intron 50.0%
GCTCATGCAGAAACAGCTCT+CGG + chr5_2:43621270-43621289 None:intergenic 50.0%
!! AGCTGTTTCTGCATGAGCCA+TGG - chr5_2:43621272-43621291 Msa0771290:intron 50.0%
!! GCTGTTTCTGCATGAGCCAT+GGG - chr5_2:43621273-43621292 Msa0771290:intron 50.0%
CGTGAGTCATCAAACACCCA+TGG + chr5_2:43621292-43621311 None:intergenic 50.0%
TGGTTGGGGGCCTTTGTAGT+TGG - chr5_2:43618879-43618898 Msa0771290:three_prime_UTR 55.0%
GGTTGGGGGCCTTTGTAGTT+GGG - chr5_2:43618880-43618899 Msa0771290:three_prime_UTR 55.0%
GGCCTTTGTAGTTGGGTGGT+GGG - chr5_2:43618887-43618906 Msa0771290:three_prime_UTR 55.0%
GTGGGAAACGAGAACGCGAT+GGG - chr5_2:43618905-43618924 Msa0771290:three_prime_UTR 55.0%
GGCGCTTCCATGAGAGGAAT+TGG + chr5_2:43618988-43619007 None:intergenic 55.0%
TTGACGCAACACATGGGAGG+AGG - chr5_2:43619738-43619757 Msa0771290:intron 55.0%
CAACACATGGGAGGAGGAGT+GGG - chr5_2:43619744-43619763 Msa0771290:intron 55.0%
AACACATGGGAGGAGGAGTG+GGG - chr5_2:43619745-43619764 Msa0771290:intron 55.0%
GAGGAGGAGTGGGGTGAAAT+AGG - chr5_2:43619754-43619773 Msa0771290:intron 55.0%
TTTGTCCGAGCGAATGAGGG+TGG - chr5_2:43620640-43620659 Msa0771290:intron 55.0%
GTACACCACCCTCATTCGCT+CGG + chr5_2:43620648-43620667 None:intergenic 55.0%
TGTACCTGCACACACTCCGA+CGG - chr5_2:43620663-43620682 Msa0771290:intron 55.0%
ACTCCGACGGTCAAGTCAGT+AGG - chr5_2:43620676-43620695 Msa0771290:intron 55.0%
CGTACCTAACCCAGTGTCCA+TGG - chr5_2:43620736-43620755 Msa0771290:intron 55.0%
GACCTCATCCAGGACATCAC+AGG + chr5_2:43620917-43620936 None:intergenic 55.0%
CCATGGTATGGACCTCATCC+AGG + chr5_2:43620927-43620946 None:intergenic 55.0%
CCTGGATGAGGTCCATACCA+TGG - chr5_2:43620924-43620943 Msa0771290:intron 55.0%
GGCCCATAAGGACCATGGTA+TGG + chr5_2:43620939-43620958 None:intergenic 55.0%
ACAATCCAGGAAACGGGCAG+AGG - chr5_2:43621213-43621232 Msa0771290:intron 55.0%
CTCTCGGCCGTTCATTTGGA+CGG + chr5_2:43621254-43621273 None:intergenic 55.0%
ACAGCTCTCGGCCGTTCATT+TGG + chr5_2:43621258-43621277 None:intergenic 55.0%
TGGGGGCCTTTGTAGTTGGG+TGG - chr5_2:43618883-43618902 Msa0771290:three_prime_UTR 60.0%
GGGCCTTTGTAGTTGGGTGG+TGG - chr5_2:43618886-43618905 Msa0771290:three_prime_UTR 60.0%
GGTGGGAAACGAGAACGCGA+TGG - chr5_2:43618904-43618923 Msa0771290:three_prime_UTR 60.0%
CCTTGTGGCGCTTCCATGAG+AGG + chr5_2:43618994-43619013 None:intergenic 60.0%
!! CCTCTCATGGAAGCGCCACA+AGG - chr5_2:43618991-43619010 Msa0771290:three_prime_UTR 60.0%
GCAACACATGGGAGGAGGAG+TGG - chr5_2:43619743-43619762 Msa0771290:intron 60.0%
TAGTGGCCCTGATGGCTTCC+TGG - chr5_2:43620172-43620191 Msa0771290:intron 60.0%
AGCATGGCGACCCCACTTAG+TGG + chr5_2:43620253-43620272 None:intergenic 60.0%
TTGACCGTCGGAGTGTGTGC+AGG + chr5_2:43620670-43620689 None:intergenic 60.0%
!! GCGCCTACTGACTTGACCGT+CGG + chr5_2:43620682-43620701 None:intergenic 60.0%
GGTTGGGTCTCGGCCTAAAC+CGG - chr5_2:43620990-43621009 Msa0771290:intron 60.0%
CGGGCAGAGGATATTGCCGC+TGG - chr5_2:43621226-43621245 Msa0771290:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 43618854 43621672 43618854 ID=Msa0771290;Name=Msa0771290
chr5_2 mRNA 43618854 43621672 43618854 ID=Msa0771290-mRNA-1;Parent=Msa0771290;Name=Msa0771290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=69|1|1|1|1|1|2|354|74
chr5_2 exon 43618854 43619342 43618854 ID=Msa0771290-mRNA-1:exon:13423;Parent=Msa0771290-mRNA-1
chr5_2 exon 43621514 43621672 43621514 ID=Msa0771290-mRNA-1:exon:13422;Parent=Msa0771290-mRNA-1
chr5_2 five_prime_UTR 43621604 43621672 43621604 ID=Msa0771290-mRNA-1:five_prime_utr;Parent=Msa0771290-mRNA-1
chr5_2 CDS 43621514 43621603 43621514 ID=Msa0771290-mRNA-1:cds;Parent=Msa0771290-mRNA-1
chr5_2 CDS 43619208 43619342 43619208 ID=Msa0771290-mRNA-1:cds;Parent=Msa0771290-mRNA-1
chr5_2 three_prime_UTR 43618854 43619207 43618854 ID=Msa0771290-mRNA-1:three_prime_utr;Parent=Msa0771290-mRNA-1
Gene Sequence

>Msa0771290

ATGGGAGCAGAAAGTGAAAAGAAAGAAGAAGAGAGACCAATAAGAGCTTCTCTCACACAAGACCAATTCCTCTCATGGAAGCGCCACAAGGATGCTGCTGAATCAGCCAGAAAAGCTGAACAATCCAGGAAACGGGCAGAGGATATTGCCGCTGGAACCGTCCAAATGAACGGCCGAGAGCTGTTTCTGCATGAGCCATGGGTGTTTGATGACTCACGTTTTTGA
Protein sequence

>Msa0771290

MGAESEKKEEERPIRASLTQDQFLSWKRHKDAAESARKAEQSRKRAEDIAAGTVQMNGRELFLHEPWVFDDSRF*