AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0773060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773060 MtrunA17_Chr5g0426551 99.615 260 1 0 1 260 1 260 0.0 532
Msa0773060 MtrunA17_Chr3g0132301 90.385 260 25 0 1 260 1 260 9.36e-177 486
Msa0773060 MtrunA17_Chr3g0133161 75.949 237 56 1 8 243 11 247 1.98e-135 382
Msa0773060 MtrunA17_Chr2g0325591 72.016 243 66 1 8 250 7 247 7.25e-129 365
Msa0773060 MtrunA17_Chr2g0283791 69.020 255 72 3 6 256 3 254 4.36e-126 358
Msa0773060 MtrunA17_Chr8g0379011 71.967 239 65 1 8 246 7 243 1.50e-125 356
Msa0773060 MtrunA17_Chr5g0418971 70.325 246 71 1 3 248 2 245 1.71e-124 354
Msa0773060 MtrunA17_Chr2g0316011 66.154 260 85 2 1 259 1 258 8.43e-124 353
Msa0773060 MtrunA17_Chr4g0043141 64.017 239 65 2 27 246 1 237 1.15e-105 306
Msa0773060 MtrunA17_Chr1g0164601 58.723 235 91 4 8 240 11 241 7.81e-91 269
Msa0773060 MtrunA17_Chr3g0082111 58.298 235 92 4 8 240 41 271 5.11e-90 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773060 AT1G78300.1 90.157 254 24 1 8 260 5 258 1.78e-169 468
Msa0773060 AT1G35160.1 85.985 264 33 2 1 260 1 264 1.87e-167 464
Msa0773060 AT4G09000.1 88.933 253 26 1 1 251 1 253 2.54e-166 461
Msa0773060 AT1G35160.2 77.740 292 33 3 1 260 1 292 3.52e-161 449
Msa0773060 AT4G09000.2 89.344 244 24 1 1 242 1 244 2.71e-160 447
Msa0773060 AT5G38480.1 81.496 254 46 1 6 258 2 255 3.30e-154 429
Msa0773060 AT3G02520.1 80.784 255 48 1 6 259 3 257 1.34e-152 426
Msa0773060 AT3G02520.2 80.784 255 48 1 6 259 3 257 1.34e-152 426
Msa0773060 AT5G38480.3 81.496 254 45 2 6 258 2 254 1.02e-151 423
Msa0773060 AT5G38480.2 81.496 254 45 2 6 258 2 254 1.02e-151 423
Msa0773060 AT5G10450.2 79.508 244 49 1 4 246 3 246 3.72e-145 406
Msa0773060 AT5G10450.1 79.668 241 48 1 4 243 3 243 8.60e-145 405
Msa0773060 AT5G10450.4 77.510 249 55 1 4 251 3 251 8.93e-145 406
Msa0773060 AT5G10450.3 80.000 240 47 1 4 242 3 242 5.64e-144 404
Msa0773060 AT5G16050.1 79.310 261 50 2 3 260 2 261 3.35e-143 402
Msa0773060 AT5G16050.2 79.310 261 50 2 3 260 2 261 3.35e-143 402
Msa0773060 AT5G65430.2 78.138 247 52 2 1 246 1 246 2.19e-142 399
Msa0773060 AT5G65430.1 78.279 244 51 2 1 243 1 243 6.17e-142 398
Msa0773060 AT5G65430.3 78.601 243 50 2 1 242 1 242 2.83e-141 397
Msa0773060 AT1G26480.1 74.180 244 60 3 8 250 10 251 7.56e-129 366
Msa0773060 AT2G42590.3 70.539 241 69 1 8 248 7 245 3.36e-122 349
Msa0773060 AT2G42590.1 70.539 241 69 1 8 248 7 245 3.39e-122 349
Msa0773060 AT2G42590.2 70.833 240 68 1 8 247 7 244 9.15e-122 348
Msa0773060 AT1G34760.2 71.008 238 67 1 8 245 5 240 3.80e-121 346
Msa0773060 AT1G34760.1 71.008 238 67 1 8 245 5 240 4.76e-121 346
Msa0773060 AT1G22300.2 68.254 252 73 2 8 259 5 249 6.77e-117 335
Msa0773060 AT1G22300.1 68.254 252 73 2 8 259 5 249 6.77e-117 335
Msa0773060 AT1G22300.3 69.874 239 70 1 8 246 5 241 1.40e-115 332
Msa0773060 AT1G78220.1 48.927 233 116 2 8 239 5 235 1.31e-73 225
Msa0773060 AT1G22290.2 45.933 209 91 6 12 217 9 198 4.64e-48 159
Msa0773060 AT1G22290.1 45.631 206 90 6 12 214 9 195 6.36e-48 157
Msa0773060 AT2G10450.1 71.429 49 14 0 207 255 16 64 7.81e-18 76.3

Find 75 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTGTAAAAGATGAATT+TGG 0.144512 5_2:+47722378 Msa0773060:five_prime_UTR
ATGGCAAATCCGTACAAATT+TGG 0.171832 5_2:+47722464 Msa0773060:five_prime_UTR
TTCAAATTCAAATGTATAAA+TGG 0.227294 5_2:+47722445 Msa0773060:five_prime_UTR
TGCTGCTTCTGGTGATTCTA+AGG 0.228252 5_2:+47723016 Msa0773060:CDS
AAGAATACCGTTACAGATAT+TGG 0.234430 5_2:-47722964 None:intergenic
TACTGCTCATCGGCGCCTTT+TGG 0.244944 5_2:-47724675 None:intergenic
TGGCAAATCCGTACAAATTT+GGG 0.259825 5_2:+47722465 Msa0773060:five_prime_UTR
TTGATTCCTTCTGCTGCTTC+TGG 0.276388 5_2:+47723005 Msa0773060:CDS
CGATGAGGCTATTGCTGAAT+TGG 0.295305 5_2:+47724434 Msa0773060:CDS
AATTGAAGAACAAACTAAAA+TGG 0.306511 5_2:-47724841 None:intergenic
CGAAGAGATGGTCGATTTCA+TGG 0.314624 5_2:+47722737 Msa0773060:CDS
AAATTTCTATCATATGAAAT+TGG 0.325146 5_2:+47724906 Msa0773060:three_prime_UTR
TGCTTGTGGCCTTGCAAAAC+AGG 0.353700 5_2:+47723969 Msa0773060:CDS
GATAATCACCTTTCATCTTA+AGG 0.367061 5_2:-47723046 None:intergenic
AAATTGTAAAAGATGAATTT+GGG 0.395537 5_2:+47722379 Msa0773060:five_prime_UTR
TTGTTTGGGTTTCAGGATGA+TGG 0.406709 5_2:+47724627 Msa0773060:intron
TTCGTAACGTTCGGCTTGTT+CGG 0.410967 5_2:-47722718 None:intergenic
TTTATGATATATGGATGCAC+TGG 0.414849 5_2:+47724817 Msa0773060:three_prime_UTR
AGAGCAAGACCAAGCCTGAT+TGG 0.420669 5_2:-47723901 None:intergenic
TCCGTGATAACCTCACCCTT+TGG 0.424479 5_2:+47724508 Msa0773060:CDS
GACCATCTCTTCGTAACGTT+CGG 0.437327 5_2:-47722727 None:intergenic
AGATGAAAGGTGATTATCAT+AGG 0.447012 5_2:+47723051 Msa0773060:CDS
TACCACCAACTCACCCAATC+AGG 0.450085 5_2:+47723887 Msa0773060:CDS
GCAAGGCCACAAGCACGATC+AGG 0.466255 5_2:-47723961 None:intergenic
ATTGCTGAATTGGATACATT+GGG 0.469687 5_2:+47724444 Msa0773060:CDS
GCTTTCTTCCTTCTGTTCGA+TGG 0.473046 5_2:-47722877 None:intergenic
TACTGCTTATAAAGCTGCTC+AGG 0.475422 5_2:+47723130 Msa0773060:CDS
ATCAAAGAAGCCGCACCAAA+AGG 0.476807 5_2:+47724660 Msa0773060:CDS
GAGCAAGACCAAGCCTGATT+GGG 0.484933 5_2:-47723900 None:intergenic
AAACTCTTCACTCTTCAGAC+AGG 0.486125 5_2:+47725043 Msa0773060:three_prime_UTR
TTGTGAGAAATGTTTGTAAA+GGG 0.487958 5_2:-47722579 None:intergenic
TATTGCTGAATTGGATACAT+TGG 0.488219 5_2:+47724443 Msa0773060:CDS
TTGCCATGGATCGATGAATC+TGG 0.497923 5_2:-47722650 None:intergenic
CTCTTCACGCGGTGTTGGTG+CGG 0.499045 5_2:-47722673 None:intergenic
GGTGAGTTCTTCGCTTTCGA+CGG 0.507397 5_2:-47722775 None:intergenic
CGGTGTTGGTGCGGTTGCCA+TGG 0.510579 5_2:-47722664 None:intergenic
TCGGAGCAAGACGTGCTTCG+TGG 0.514368 5_2:+47722841 Msa0773060:CDS
TTTGTGAGAAATGTTTGTAA+AGG 0.520519 5_2:-47722580 None:intergenic
AACTCTCCTGATCGTGCTTG+TGG 0.523777 5_2:+47723955 Msa0773060:CDS
GGATCGATGAATCTGGTTGA+AGG 0.526176 5_2:-47722643 None:intergenic
CCTTTGGACCTCTGACATGC+AGG 0.533382 5_2:+47724524 Msa0773060:CDS
TGAGAAATGTTTGTAAAGGG+TGG 0.536454 5_2:-47722576 None:intergenic
TTCTTCGCTTTCGACGGCGG+CGG 0.538091 5_2:-47722769 None:intergenic
GCGTGAAGAGTTCGTCTACA+TGG 0.544670 5_2:+47722686 Msa0773060:CDS
CCAACTCACCCAATCAGGCT+TGG 0.548958 5_2:+47723892 Msa0773060:CDS
CATCTCTTCCATCGAACAGA+AGG 0.549390 5_2:+47722869 Msa0773060:CDS
CCTGCATGTCAGAGGTCCAA+AGG 0.556945 5_2:-47724524 None:intergenic
CTGCATGTCAGAGGTCCAAA+GGG 0.558806 5_2:-47724523 None:intergenic
ACGAACTCTTCACGCGGTGT+TGG 0.561299 5_2:-47722678 None:intergenic
AGCCTGATTGGGTGAGTTGG+TGG 0.564969 5_2:-47723889 None:intergenic
TGAATTGGATACATTGGGAG+AGG 0.566946 5_2:+47724449 Msa0773060:CDS
AACTCTTCACTCTTCAGACA+GGG 0.571026 5_2:+47725044 Msa0773060:three_prime_UTR
TCCAAAGGGTGAGGTTATCA+CGG 0.578031 5_2:-47724509 None:intergenic
CCAAGCCTGATTGGGTGAGT+TGG 0.579199 5_2:-47723892 None:intergenic
CAACCAGATTCATCGATCCA+TGG 0.582939 5_2:+47722647 Msa0773060:exon
GAGTTCTTCGCTTTCGACGG+CGG 0.587801 5_2:-47722772 None:intergenic
GAACTTTCCAATATCTGTAA+CGG 0.597556 5_2:+47722957 Msa0773060:CDS
GGCAAATCCGTACAAATTTG+GGG 0.601322 5_2:+47722466 Msa0773060:five_prime_UTR
TGTCAGAGGTCCAAAGGGTG+AGG 0.606809 5_2:-47724518 None:intergenic
TTATGATATATGGATGCACT+GGG 0.612064 5_2:+47724818 Msa0773060:three_prime_UTR
AGATCTGTAATCACGAATCA+CGG 0.615306 5_2:-47722922 None:intergenic
GAATCACCAGAAGCAGCAGA+AGG 0.617542 5_2:-47723011 None:intergenic
TATCTTGCTGAGTTTAAGAG+TGG 0.621733 5_2:+47723074 Msa0773060:CDS
AGCCGAACGTTACGAAGAGA+TGG 0.627906 5_2:+47722725 Msa0773060:CDS
ACGATATGATTACTGCTCAT+CGG 0.628913 5_2:-47724685 None:intergenic
ATATCATACCTGCATGTCAG+AGG 0.643750 5_2:-47724532 None:intergenic
ATTGGGAGAGGAATCATACA+AGG 0.653137 5_2:+47724461 Msa0773060:CDS
GAGGAGGTTACGTTCTTCGA+CGG 0.656962 5_2:-47722796 None:intergenic
AAAGGGTGGATGTGAATGAG+AGG 0.658640 5_2:-47722562 None:intergenic
TAACATGTTCATCGTTACCA+CGG 0.672519 5_2:-47722899 None:intergenic
CCTTTCAAAACATGATACAG+AGG 0.674464 5_2:-47724735 None:intergenic
GAACAGAAGGAAGAAAGCCG+TGG 0.676297 5_2:+47722882 Msa0773060:CDS
GCAAATCCGTACAAATTTGG+GGG 0.683328 5_2:+47722467 Msa0773060:five_prime_UTR
TATTCATTAGGCTTTCGATG+AGG 0.689894 5_2:+47724419 Msa0773060:intron
ATGTAGACGAACTCTTCACG+CGG 0.772962 5_2:-47722684 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTGTAAAAGATGAATT+TGG + chr5_2:47722378-47722397 Msa0773060:five_prime_UTR 15.0%
!! AAATTGTAAAAGATGAATTT+GGG + chr5_2:47722379-47722398 Msa0773060:five_prime_UTR 15.0%
!!! TCTTATTTCAATTGTTTTTT+TGG + chr5_2:47722409-47722428 Msa0773060:intron 15.0%
!!! CTTATTTCAATTGTTTTTTT+GGG + chr5_2:47722410-47722429 Msa0773060:intron 15.0%
!! TTCAAATTCAAATGTATAAA+TGG + chr5_2:47722445-47722464 Msa0773060:five_prime_UTR 15.0%
!!! TAATATGATTTGTGTTAATT+AGG + chr5_2:47723368-47723387 Msa0773060:intron 15.0%
!! TCTTAATTAGGTTAAAATAA+TGG + chr5_2:47723480-47723499 Msa0773060:intron 15.0%
!!! ATTTTGGTTTATATTTTGTT+TGG + chr5_2:47724612-47724631 Msa0773060:intron 15.0%
!!! TTTTGGTTTATATTTTGTTT+GGG + chr5_2:47724613-47724632 Msa0773060:intron 15.0%
!! AAATTTCTATCATATGAAAT+TGG + chr5_2:47724906-47724925 Msa0773060:three_prime_UTR 15.0%
!!! TCATCTTTTACAATTTTCTT+AGG - chr5_2:47722375-47722394 None:intergenic 20.0%
!!! GATTTGATGATTTCTTAATT+AGG + chr5_2:47723468-47723487 Msa0773060:intron 20.0%
!! AAAATGAAAGTCTTAAATGA+TGG + chr5_2:47723567-47723586 Msa0773060:intron 20.0%
!! ACTACAAACTTTAATTGAAA+AGG - chr5_2:47724063-47724082 None:intergenic 20.0%
!!! TTTTCAATTAAAGTTTGTAG+TGG + chr5_2:47724062-47724081 Msa0773060:intron 20.0%
!! TAATTTCATTAACATGTTTG+TGG + chr5_2:47724223-47724242 Msa0773060:intron 20.0%
!! AATTTCATTAACATGTTTGT+GGG + chr5_2:47724224-47724243 Msa0773060:intron 20.0%
!! AATTCACATCAACATTAAAA+AGG - chr5_2:47724270-47724289 None:intergenic 20.0%
!! TAATGAATATAGAAAAGAAC+AGG - chr5_2:47724408-47724427 None:intergenic 20.0%
!!! TGTTCTTTTCTATATTCATT+AGG + chr5_2:47724407-47724426 Msa0773060:intron 20.0%
!!! CATATTCTGTTTATTGATTT+TGG + chr5_2:47724596-47724615 Msa0773060:intron 20.0%
!!! ATGGTCTATTTTATGATATA+TGG + chr5_2:47724808-47724827 Msa0773060:three_prime_UTR 20.0%
!! AATTGAAGAACAAACTAAAA+TGG - chr5_2:47724844-47724863 None:intergenic 20.0%
!!! GGGTTAACTATTTTTAATTC+AGG - chr5_2:47722507-47722526 None:intergenic 25.0%
!!! ATAGCAGTTTTTTGTTTTAG+TGG - chr5_2:47722537-47722556 None:intergenic 25.0%
! TTGTGAGAAATGTTTGTAAA+GGG - chr5_2:47722582-47722601 None:intergenic 25.0%
! TTTGTGAGAAATGTTTGTAA+AGG - chr5_2:47722583-47722602 None:intergenic 25.0%
! GAGATCTAACGTTATATTTA+TGG + chr5_2:47723168-47723187 Msa0773060:intron 25.0%
! GCAATATTTCAAACAAAGAA+TGG - chr5_2:47723278-47723297 None:intergenic 25.0%
! TTTAGGTAAGGGAAATTTAA+CGG + chr5_2:47723312-47723331 Msa0773060:intron 25.0%
!!! TTTTCAAATGAGTTGAATGT+AGG + chr5_2:47723524-47723543 Msa0773060:intron 25.0%
!! TTTCAAATGAGTTGAATGTA+GGG + chr5_2:47723525-47723544 Msa0773060:intron 25.0%
!!! TGTAGGGTATTGTTTATTTT+TGG + chr5_2:47723541-47723560 Msa0773060:intron 25.0%
!!! TTTAATTTGACTCTGTTTGT+AGG + chr5_2:47723846-47723865 Msa0773060:intron 25.0%
! TATTCTAGATACCGAATAAT+GGG + chr5_2:47723996-47724015 Msa0773060:intron 25.0%
! ACTACAGACTTTAATTGAAA+AGG - chr5_2:47724124-47724143 None:intergenic 25.0%
!! TTTTCAATTAAAGTCTGTAG+TGG + chr5_2:47724123-47724142 Msa0773060:intron 25.0%
!! AATGTTGATGTGAATTTGTT+AGG + chr5_2:47724274-47724293 Msa0773060:intron 25.0%
!! TGATTCTGTTCTGTTATTTA+TGG + chr5_2:47724362-47724381 Msa0773060:intron 25.0%
!!! TTATATTTTGTTTGGGTTTC+AGG + chr5_2:47724620-47724639 Msa0773060:intron 25.0%
!!! CAGTTTTTTGTTTTAGTGGA+CGG - chr5_2:47722533-47722552 None:intergenic 30.0%
GAACTTTCCAATATCTGTAA+CGG + chr5_2:47722957-47722976 Msa0773060:CDS 30.0%
AAGAATACCGTTACAGATAT+TGG - chr5_2:47722967-47722986 None:intergenic 30.0%
! GTTTTCTACCTTAAGATGAA+AGG + chr5_2:47723038-47723057 Msa0773060:CDS 30.0%
GATAATCACCTTTCATCTTA+AGG - chr5_2:47723049-47723068 None:intergenic 30.0%
AGATGAAAGGTGATTATCAT+AGG + chr5_2:47723051-47723070 Msa0773060:CDS 30.0%
TTAGATCTCAGAGAAAAAGA+TGG - chr5_2:47723157-47723176 None:intergenic 30.0%
! TCTATAACTGACTTTAGGTA+AGG + chr5_2:47723300-47723319 Msa0773060:intron 30.0%
! CTATAACTGACTTTAGGTAA+GGG + chr5_2:47723301-47723320 Msa0773060:intron 30.0%
! TTTAACGGAGTTTTTGTGTT+TGG + chr5_2:47723327-47723346 Msa0773060:intron 30.0%
GTATTCTAGATACCGAATAA+TGG + chr5_2:47723995-47724014 Msa0773060:intron 30.0%
TATTTGTTCCTCAACAAGTT+TGG + chr5_2:47724326-47724345 Msa0773060:intron 30.0%
TATTGCTGAATTGGATACAT+TGG + chr5_2:47724443-47724462 Msa0773060:CDS 30.0%
ATTGCTGAATTGGATACATT+GGG + chr5_2:47724444-47724463 Msa0773060:CDS 30.0%
!!! CTCTGTATCATGTTTTGAAA+GGG + chr5_2:47724736-47724755 Msa0773060:three_prime_UTR 30.0%
!!! TCTGTATCATGTTTTGAAAG+GGG + chr5_2:47724737-47724756 Msa0773060:three_prime_UTR 30.0%
TTTATGATATATGGATGCAC+TGG + chr5_2:47724817-47724836 Msa0773060:three_prime_UTR 30.0%
TTATGATATATGGATGCACT+GGG + chr5_2:47724818-47724837 Msa0773060:three_prime_UTR 30.0%
!!! TCAGCTGATTTTTTTCATTG+AGG + chr5_2:47724958-47724977 Msa0773060:three_prime_UTR 30.0%
ATGGCAAATCCGTACAAATT+TGG + chr5_2:47722464-47722483 Msa0773060:five_prime_UTR 35.0%
TGGCAAATCCGTACAAATTT+GGG + chr5_2:47722465-47722484 Msa0773060:five_prime_UTR 35.0%
AATTTGCCCCCAAATTTGTA+CGG - chr5_2:47722476-47722495 None:intergenic 35.0%
TGAGAAATGTTTGTAAAGGG+TGG - chr5_2:47722579-47722598 None:intergenic 35.0%
TAACATGTTCATCGTTACCA+CGG - chr5_2:47722902-47722921 None:intergenic 35.0%
AGATCTGTAATCACGAATCA+CGG - chr5_2:47722925-47722944 None:intergenic 35.0%
TATCTTGCTGAGTTTAAGAG+TGG + chr5_2:47723074-47723093 Msa0773060:CDS 35.0%
! TGCGATCTATAACTGACTTT+AGG + chr5_2:47723295-47723314 Msa0773060:intron 35.0%
ATATTGACACCTCTGATCAA+AGG + chr5_2:47723694-47723713 Msa0773060:intron 35.0%
TTGAACATAATCATGTGCGT+CGG - chr5_2:47723743-47723762 None:intergenic 35.0%
! GTGTTATTATTGCGTCAGTT+TGG + chr5_2:47723799-47723818 Msa0773060:intron 35.0%
! GAATACTAACCTGTTTTGCA+AGG - chr5_2:47723981-47724000 None:intergenic 35.0%
GAACATCATCTCCCATTATT+CGG - chr5_2:47724010-47724029 None:intergenic 35.0%
AATGGGAGATGATGTTCATT+TGG + chr5_2:47724013-47724032 Msa0773060:intron 35.0%
!! GATGTGAATTTGTTAGGTTC+TGG + chr5_2:47724280-47724299 Msa0773060:intron 35.0%
TATTCATTAGGCTTTCGATG+AGG + chr5_2:47724419-47724438 Msa0773060:intron 35.0%
ACGATATGATTACTGCTCAT+CGG - chr5_2:47724688-47724707 None:intergenic 35.0%
CCTTTCAAAACATGATACAG+AGG - chr5_2:47724738-47724757 None:intergenic 35.0%
!! CCTCTGTATCATGTTTTGAA+AGG + chr5_2:47724735-47724754 Msa0773060:three_prime_UTR 35.0%
! GTTGATGCTACAGAGATTTT+AGG + chr5_2:47724780-47724799 Msa0773060:three_prime_UTR 35.0%
! ACAGAGATTTTAGGCATTCA+TGG + chr5_2:47724789-47724808 Msa0773060:three_prime_UTR 35.0%
GGCAAATCCGTACAAATTTG+GGG + chr5_2:47722466-47722485 Msa0773060:five_prime_UTR 40.0%
GCAAATCCGTACAAATTTGG+GGG + chr5_2:47722467-47722486 Msa0773060:five_prime_UTR 40.0%
!!! TTTGTTTTAGTGGACGGTGA+GGG - chr5_2:47722527-47722546 None:intergenic 40.0%
!!! TTTTGTTTTAGTGGACGGTG+AGG - chr5_2:47722528-47722547 None:intergenic 40.0%
TACTGCTTATAAAGCTGCTC+AGG + chr5_2:47723130-47723149 Msa0773060:CDS 40.0%
!! ATGCTACACCAAACTTGTTG+AGG - chr5_2:47724337-47724356 None:intergenic 40.0%
TGAATTGGATACATTGGGAG+AGG + chr5_2:47724449-47724468 Msa0773060:CDS 40.0%
ATTGGGAGAGGAATCATACA+AGG + chr5_2:47724461-47724480 Msa0773060:CDS 40.0%
ATATCATACCTGCATGTCAG+AGG - chr5_2:47724535-47724554 None:intergenic 40.0%
! TTGTTTGGGTTTCAGGATGA+TGG + chr5_2:47724627-47724646 Msa0773060:intron 40.0%
!!! TCATGTTTTGAAAGGGGAGA+AGG + chr5_2:47724743-47724762 Msa0773060:three_prime_UTR 40.0%
AAACTCTTCACTCTTCAGAC+AGG + chr5_2:47725043-47725062 Msa0773060:three_prime_UTR 40.0%
AACTCTTCACTCTTCAGACA+GGG + chr5_2:47725044-47725063 Msa0773060:three_prime_UTR 40.0%
AAAGGGTGGATGTGAATGAG+AGG - chr5_2:47722565-47722584 None:intergenic 45.0%
GGATCGATGAATCTGGTTGA+AGG - chr5_2:47722646-47722665 None:intergenic 45.0%
CAACCAGATTCATCGATCCA+TGG + chr5_2:47722647-47722666 Msa0773060:exon 45.0%
TTGCCATGGATCGATGAATC+TGG - chr5_2:47722653-47722672 None:intergenic 45.0%
ATGTAGACGAACTCTTCACG+CGG - chr5_2:47722687-47722706 None:intergenic 45.0%
! TTCGTAACGTTCGGCTTGTT+CGG - chr5_2:47722721-47722740 None:intergenic 45.0%
GACCATCTCTTCGTAACGTT+CGG - chr5_2:47722730-47722749 None:intergenic 45.0%
! CGAAGAGATGGTCGATTTCA+TGG + chr5_2:47722737-47722756 Msa0773060:CDS 45.0%
! GATGACGTTTTTGTAGGCGA+CGG - chr5_2:47722823-47722842 None:intergenic 45.0%
GTCGCCTACAAAAACGTCAT+CGG + chr5_2:47722822-47722841 Msa0773060:CDS 45.0%
! TGCTCCGATGACGTTTTTGT+AGG - chr5_2:47722829-47722848 None:intergenic 45.0%
CATCTCTTCCATCGAACAGA+AGG + chr5_2:47722869-47722888 Msa0773060:CDS 45.0%
GCTTTCTTCCTTCTGTTCGA+TGG - chr5_2:47722880-47722899 None:intergenic 45.0%
! TTGATTCCTTCTGCTGCTTC+TGG + chr5_2:47723005-47723024 Msa0773060:CDS 45.0%
! TGCTGCTTCTGGTGATTCTA+AGG + chr5_2:47723016-47723035 Msa0773060:CDS 45.0%
CGATGAGGCTATTGCTGAAT+TGG + chr5_2:47724434-47724453 Msa0773060:CDS 45.0%
TCCAAAGGGTGAGGTTATCA+CGG - chr5_2:47724512-47724531 None:intergenic 45.0%
ATCAAAGAAGCCGCACCAAA+AGG + chr5_2:47724660-47724679 Msa0773060:CDS 45.0%
GCGTGAAGAGTTCGTCTACA+TGG + chr5_2:47722686-47722705 Msa0773060:CDS 50.0%
AGCCGAACGTTACGAAGAGA+TGG + chr5_2:47722725-47722744 Msa0773060:CDS 50.0%
GGTGAGTTCTTCGCTTTCGA+CGG - chr5_2:47722778-47722797 None:intergenic 50.0%
GAGGAGGTTACGTTCTTCGA+CGG - chr5_2:47722799-47722818 None:intergenic 50.0%
GAACAGAAGGAAGAAAGCCG+TGG + chr5_2:47722882-47722901 Msa0773060:CDS 50.0%
GAATCACCAGAAGCAGCAGA+AGG - chr5_2:47723014-47723033 None:intergenic 50.0%
TACCACCAACTCACCCAATC+AGG + chr5_2:47723887-47723906 Msa0773060:CDS 50.0%
GAGCAAGACCAAGCCTGATT+GGG - chr5_2:47723903-47723922 None:intergenic 50.0%
AGAGCAAGACCAAGCCTGAT+TGG - chr5_2:47723904-47723923 None:intergenic 50.0%
AACTCTCCTGATCGTGCTTG+TGG + chr5_2:47723955-47723974 Msa0773060:CDS 50.0%
! TGCTTGTGGCCTTGCAAAAC+AGG + chr5_2:47723969-47723988 Msa0773060:CDS 50.0%
! TTGTGGGAGCTTTAGTGCAG+TGG + chr5_2:47724240-47724259 Msa0773060:intron 50.0%
! TGTGGGAGCTTTAGTGCAGT+GGG + chr5_2:47724241-47724260 Msa0773060:intron 50.0%
TCCGTGATAACCTCACCCTT+TGG + chr5_2:47724508-47724527 Msa0773060:CDS 50.0%
CTGCATGTCAGAGGTCCAAA+GGG - chr5_2:47724526-47724545 None:intergenic 50.0%
ACGAACTCTTCACGCGGTGT+TGG - chr5_2:47722681-47722700 None:intergenic 55.0%
GAGTTCTTCGCTTTCGACGG+CGG - chr5_2:47722775-47722794 None:intergenic 55.0%
! TTTTGTAGGCGACGGAGAGG+AGG - chr5_2:47722815-47722834 None:intergenic 55.0%
! CGTTTTTGTAGGCGACGGAG+AGG - chr5_2:47722818-47722837 None:intergenic 55.0%
CCAGACACGCCTTTGATCAG+AGG - chr5_2:47723706-47723725 None:intergenic 55.0%
! CCTCTGATCAAAGGCGTGTC+TGG + chr5_2:47723703-47723722 Msa0773060:intron 55.0%
! AGGCGTGTCTGGTTTCTGAC+AGG + chr5_2:47723714-47723733 Msa0773060:intron 55.0%
! GGCGTGTCTGGTTTCTGACA+GGG + chr5_2:47723715-47723734 Msa0773060:intron 55.0%
! GCGTGTCTGGTTTCTGACAG+GGG + chr5_2:47723716-47723735 Msa0773060:intron 55.0%
! AGCCTGATTGGGTGAGTTGG+TGG - chr5_2:47723892-47723911 None:intergenic 55.0%
CCAAGCCTGATTGGGTGAGT+TGG - chr5_2:47723895-47723914 None:intergenic 55.0%
CCAACTCACCCAATCAGGCT+TGG + chr5_2:47723892-47723911 Msa0773060:CDS 55.0%
TGTCAGAGGTCCAAAGGGTG+AGG - chr5_2:47724521-47724540 None:intergenic 55.0%
CCTGCATGTCAGAGGTCCAA+AGG - chr5_2:47724527-47724546 None:intergenic 55.0%
CCTTTGGACCTCTGACATGC+AGG + chr5_2:47724524-47724543 Msa0773060:CDS 55.0%
! TACTGCTCATCGGCGCCTTT+TGG - chr5_2:47724678-47724697 None:intergenic 55.0%
!! CTCTTCACGCGGTGTTGGTG+CGG - chr5_2:47722676-47722695 None:intergenic 60.0%
TTCTTCGCTTTCGACGGCGG+CGG - chr5_2:47722772-47722791 None:intergenic 60.0%
TCGGAGCAAGACGTGCTTCG+TGG + chr5_2:47722841-47722860 Msa0773060:CDS 60.0%
GCAAGGCCACAAGCACGATC+AGG - chr5_2:47723964-47723983 None:intergenic 60.0%
! CTCATCGGCGCCTTTTGGTG+CGG - chr5_2:47724673-47724692 None:intergenic 60.0%
!! CGGTGTTGGTGCGGTTGCCA+TGG - chr5_2:47722667-47722686 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 47722360 47725156 47722360 ID=Msa0773060;Name=Msa0773060
chr5_2 mRNA 47722360 47725156 47722360 ID=Msa0773060-mRNA-1;Parent=Msa0773060;Name=Msa0773060-mRNA-1;_AED=0.02;_eAED=0.02;_QI=286|1|1|1|0.75|0.6|5|458|260
chr5_2 exon 47722360 47722410 47722360 ID=Msa0773060-mRNA-1:exon:14261;Parent=Msa0773060-mRNA-1
chr5_2 exon 47722431 47723151 47722431 ID=Msa0773060-mRNA-1:exon:14262;Parent=Msa0773060-mRNA-1
chr5_2 exon 47723868 47723990 47723868 ID=Msa0773060-mRNA-1:exon:14263;Parent=Msa0773060-mRNA-1
chr5_2 exon 47724429 47724545 47724429 ID=Msa0773060-mRNA-1:exon:14264;Parent=Msa0773060-mRNA-1
chr5_2 exon 47724642 47725156 47724642 ID=Msa0773060-mRNA-1:exon:14265;Parent=Msa0773060-mRNA-1
chr5_2 five_prime_UTR 47722360 47722410 47722360 ID=Msa0773060-mRNA-1:five_prime_utr;Parent=Msa0773060-mRNA-1
chr5_2 five_prime_UTR 47722431 47722665 47722431 ID=Msa0773060-mRNA-1:five_prime_utr;Parent=Msa0773060-mRNA-1
chr5_2 CDS 47722666 47723151 47722666 ID=Msa0773060-mRNA-1:cds;Parent=Msa0773060-mRNA-1
chr5_2 CDS 47723868 47723990 47723868 ID=Msa0773060-mRNA-1:cds;Parent=Msa0773060-mRNA-1
chr5_2 CDS 47724429 47724545 47724429 ID=Msa0773060-mRNA-1:cds;Parent=Msa0773060-mRNA-1
chr5_2 CDS 47724642 47724698 47724642 ID=Msa0773060-mRNA-1:cds;Parent=Msa0773060-mRNA-1
chr5_2 three_prime_UTR 47724699 47725156 47724699 ID=Msa0773060-mRNA-1:three_prime_utr;Parent=Msa0773060-mRNA-1
Gene Sequence

>Msa0773060

ATGGCAACCGCACCAACACCGCGTGAAGAGTTCGTCTACATGGCGAAACTCGCCGAACAAGCCGAACGTTACGAAGAGATGGTCGATTTCATGGAAAAAGTAACCGCCGCCGTCGAAAGCGAAGAACTCACCGTCGAAGAACGTAACCTCCTCTCCGTCGCCTACAAAAACGTCATCGGAGCAAGACGTGCTTCGTGGCGTATCATCTCTTCCATCGAACAGAAGGAAGAAAGCCGTGGTAACGATGAACATGTTACCGTGATTCGTGATTACAGATCTAAGATCGAAGCTGAACTTTCCAATATCTGTAACGGTATTCTTAAGCTTCTTGATTCTCGCTTGATTCCTTCTGCTGCTTCTGGTGATTCTAAGGTTTTCTACCTTAAGATGAAAGGTGATTATCATAGGTATCTTGCTGAGTTTAAGAGTGGCGCTGAGCGTAAAGATGCTGCTGAAAGTACTCTTACTGCTTATAAAGCTGCTCAGGACATTGCTAACTCTGAGCTACCACCAACTCACCCAATCAGGCTTGGTCTTGCTCTGAACTTCTCAGTGTTTTACTATGAGATTCTTAACTCTCCTGATCGTGCTTGTGGCCTTGCAAAACAGGCTTTCGATGAGGCTATTGCTGAATTGGATACATTGGGAGAGGAATCATACAAGGACAGCACTTTGATCATGCAACTTCTCCGTGATAACCTCACCCTTTGGACCTCTGACATGCAGGATGATGGTGCAGATGAAATCAAAGAAGCCGCACCAAAAGGCGCCGATGAGCAGTAA
Protein sequence

>Msa0773060

MATAPTPREEFVYMAKLAEQAERYEEMVDFMEKVTAAVESEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNDEHVTVIRDYRSKIEAELSNICNGILKLLDSRLIPSAASGDSKVFYLKMKGDYHRYLAEFKSGAERKDAAESTLTAYKAAQDIANSELPPTHPIRLGLALNFSVFYYEILNSPDRACGLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQDDGADEIKEAAPKGADEQ*