AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0775600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0775600 MtrunA17_Chr5g0430101 77.679 112 25 0 1 112 71 182 5.89e-58 186
Msa0775600 MtrunA17_Chr5g0430111 72.321 112 31 0 1 112 72 183 1.65e-53 173
Msa0775600 MtrunA17_Chr5g0430091 81.633 98 18 0 1 98 72 169 5.97e-52 171
Msa0775600 MtrunA17_Chr5g0430031 72.321 112 31 0 1 112 72 183 1.13e-51 170
Msa0775600 MtrunA17_Chr5g0430081 78.571 112 24 0 1 112 70 181 1.38e-51 170
Msa0775600 MtrunA17_Chr5g0430171 76.531 98 23 0 1 98 72 169 2.05e-49 157
Msa0775600 MtrunA17_Chr5g0430131 76.531 98 23 0 1 98 72 169 3.47e-48 160
Msa0775600 MtrunA17_Chr8g0390881 66.071 112 38 0 1 112 71 182 1.00e-46 157
Msa0775600 MtrunA17_Chr6g0484841 67.890 109 35 0 1 109 72 180 1.65e-46 156
Msa0775600 MtrunA17_Chr6g0484771 61.607 112 43 0 1 112 1 112 2.99e-46 146
Msa0775600 MtrunA17_Chr6g0484811 64.286 112 40 0 1 112 72 183 1.01e-44 151
Msa0775600 MtrunA17_Chr2g0310391 47.934 121 63 0 1 121 63 183 1.88e-34 124
Msa0775600 MtrunA17_Chr2g0310381 47.934 121 63 0 1 121 63 183 9.25e-34 122
Msa0775600 MtrunA17_Chr3g0101631 57.778 90 38 0 1 90 72 161 3.18e-32 118
Msa0775600 MtrunA17_Chr3g0101601 58.889 90 37 0 1 90 72 161 3.30e-32 118
Msa0775600 MtrunA17_Chr3g0101621 58.889 90 37 0 1 90 69 158 4.82e-32 117
Msa0775600 MtrunA17_Chr8g0382901 52.679 112 51 1 1 112 527 636 1.22e-30 114
Msa0775600 MtrunA17_Chr8g0382901 48.214 112 58 0 1 112 63 174 6.35e-30 112
Msa0775600 MtrunA17_Chr1g0162811 48.624 109 54 1 1 109 73 179 1.73e-30 113
Msa0775600 MtrunA17_Chr1g0162801 48.624 109 54 1 1 109 73 179 1.09e-29 111
Msa0775600 MtrunA17_Chr3g0101741 48.624 109 54 1 1 109 1 107 1.44e-29 110
Msa0775600 MtrunA17_Chr2g0310371 55.952 84 37 0 1 84 63 146 2.55e-29 103
Msa0775600 MtrunA17_Chr4g0020041 47.706 109 55 1 1 109 73 179 2.60e-29 110
Msa0775600 MtrunA17_Chr3g0101721 49.541 109 53 1 1 109 73 179 3.18e-29 110
Msa0775600 MtrunA17_Chr5g0403321 45.455 110 58 1 3 112 74 181 6.53e-29 108
Msa0775600 MtrunA17_Chr1g0156101 45.690 116 63 0 1 116 68 183 8.71e-29 108
Msa0775600 MtrunA17_Chr5g0403351 46.226 106 55 1 1 106 72 175 1.19e-28 108
Msa0775600 MtrunA17_Chr5g0445531 49.541 109 53 1 1 109 75 181 1.33e-28 108
Msa0775600 MtrunA17_Chr3g0101581 44.643 112 60 1 1 112 72 181 2.54e-28 107
Msa0775600 MtrunA17_Chr3g0101661 48.214 112 56 1 1 112 74 183 2.59e-28 107
Msa0775600 MtrunA17_Chr3g0101611 44.545 110 59 1 1 110 73 180 5.51e-28 106
Msa0775600 MtrunA17_Chr3g0101571 45.536 112 59 1 1 112 71 180 6.27e-28 106
Msa0775600 MtrunA17_Chr5g0403331 46.226 106 55 1 1 106 73 176 6.69e-28 106
Msa0775600 MtrunA17_Chr1g0167321 46.218 119 60 2 1 119 70 184 9.40e-28 105
Msa0775600 MtrunA17_Chr3g0101751 45.872 109 57 1 1 109 78 184 1.28e-27 105
Msa0775600 MtrunA17_Chr3g0101761 45.946 111 58 1 1 111 71 179 2.31e-27 104
Msa0775600 MtrunA17_Chr3g0101831 47.706 109 55 1 1 109 73 179 2.40e-27 104
Msa0775600 MtrunA17_Chr6g0466331 46.789 109 56 1 1 109 70 176 2.61e-27 104
Msa0775600 MtrunA17_Chr3g0101821 44.954 109 58 1 1 109 71 177 3.39e-27 104
Msa0775600 MtrunA17_Chr5g0445461 44.037 109 59 1 1 109 75 181 3.45e-27 104
Msa0775600 MtrunA17_Chr3g0101651 46.789 109 56 1 1 109 74 180 5.04e-27 103
Msa0775600 MtrunA17_Chr6g0465511 44.828 116 62 1 1 116 70 183 5.10e-27 103
Msa0775600 MtrunA17_Chr3g0101701 43.966 116 63 1 1 116 74 187 5.62e-27 103
Msa0775600 MtrunA17_Chr5g0445441 46.226 106 55 1 1 106 116 219 6.35e-27 103
Msa0775600 MtrunA17_Chr3g0101811 46.789 109 56 1 1 109 73 179 1.39e-26 102
Msa0775600 MtrunA17_Chr3g0101691 47.170 106 54 1 1 106 74 177 2.68e-26 102
Msa0775600 MtrunA17_Chr3g0101711 45.536 112 59 1 1 112 73 182 3.10e-26 101
Msa0775600 MtrunA17_Chr1g0156081 42.241 116 67 0 1 116 92 207 3.16e-26 101
Msa0775600 MtrunA17_Chr8g0361471 45.794 107 56 1 1 107 70 174 5.28e-26 100
Msa0775600 MtrunA17_Chr8g0357431 50.000 90 45 0 1 90 73 162 6.86e-26 100
Msa0775600 MtrunA17_Chr3g0101671 46.789 109 56 1 1 109 74 180 8.80e-26 100
Msa0775600 MtrunA17_Chr3g0101731 44.037 109 59 1 1 109 72 178 9.11e-26 100
Msa0775600 MtrunA17_Chr4g0076161 46.809 94 50 0 1 94 75 168 1.46e-25 99.8
Msa0775600 MtrunA17_Chr5g0445451 44.340 106 57 1 1 106 75 178 1.96e-25 99.4
Msa0775600 MtrunA17_Chr5g0445421 41.284 109 62 1 1 109 149 255 2.78e-25 99.4
Msa0775600 MtrunA17_Chr5g0445751 41.284 109 62 1 1 109 80 186 2.98e-25 97.1
Msa0775600 MtrunA17_Chr3g0101801 43.119 109 60 1 1 109 78 184 3.75e-25 98.6
Msa0775600 MtrunA17_Chr2g0280261 40.708 113 65 1 1 113 70 180 4.43e-25 98.6
Msa0775600 MtrunA17_Chr5g0445411 44.086 93 52 0 1 93 82 174 5.80e-25 93.2
Msa0775600 MtrunA17_Chr5g0445381 44.954 109 58 1 1 109 106 212 8.55e-25 97.8
Msa0775600 MtrunA17_Chr1g0174441 41.964 112 65 0 1 112 91 202 9.94e-25 97.4
Msa0775600 MtrunA17_Chr5g0431641 44.037 109 59 1 1 109 75 181 1.77e-24 96.7
Msa0775600 MtrunA17_Chr8g0357421 50.000 90 45 0 1 90 73 162 1.94e-24 96.7
Msa0775600 MtrunA17_Chr5g0445341 47.059 85 44 1 1 84 1 85 4.61e-24 88.2
Msa0775600 MtrunA17_Chr5g0446151 43.243 111 57 2 1 109 77 183 6.93e-24 95.1
Msa0775600 MtrunA17_Chr1g0156061 46.316 95 51 0 1 95 91 185 8.93e-24 94.7
Msa0775600 MtrunA17_Chr4g0047631 37.500 120 72 1 1 120 84 200 1.81e-23 94.0
Msa0775600 MtrunA17_Chr6g0463801 40.179 112 65 1 1 112 73 182 1.99e-23 94.0
Msa0775600 MtrunA17_Chr4g0076181 53.247 77 36 0 1 77 1 77 2.13e-23 89.0
Msa0775600 MtrunA17_Chr3g0116681 46.591 88 47 0 3 90 77 164 2.53e-23 93.6
Msa0775600 MtrunA17_Chr6g0463791 48.810 84 43 0 1 84 73 156 3.71e-23 93.2
Msa0775600 MtrunA17_Chr6g0472311 49.412 85 43 0 1 85 76 160 5.56e-23 92.8
Msa0775600 MtrunA17_Chr6g0463611 37.500 112 69 1 1 112 87 197 6.28e-23 91.3
Msa0775600 MtrunA17_Chr5g0446161 41.284 109 62 1 1 109 77 183 6.80e-23 92.4
Msa0775600 MtrunA17_Chr5g0431671 42.202 109 61 1 1 109 76 182 1.28e-22 91.7
Msa0775600 MtrunA17_Chr4g0024771 39.286 112 66 1 1 112 75 184 1.71e-22 91.3
Msa0775600 MtrunA17_Chr7g0258221 36.792 106 67 0 1 106 69 174 1.75e-22 91.3
Msa0775600 MtrunA17_Chr4g0058201 35.714 112 72 0 1 112 63 174 3.20e-22 90.5
Msa0775600 MtrunA17_Chr3g0116741 44.330 97 54 0 3 99 88 184 4.83e-22 90.1
Msa0775600 MtrunA17_Chr1g0174461 38.655 119 71 1 1 119 71 187 6.70e-22 89.7
Msa0775600 MtrunA17_Chr4g0011881 36.207 116 74 0 1 116 66 181 7.70e-22 89.4
Msa0775600 MtrunA17_Chr4g0058231 36.697 109 69 0 1 109 63 171 9.43e-22 89.0
Msa0775600 MtrunA17_Chr4g0011861 33.621 116 77 0 1 116 65 180 1.23e-21 87.4
Msa0775600 MtrunA17_Chr4g0047691 35.246 122 76 1 1 122 87 205 1.52e-21 88.6
Msa0775600 MtrunA17_Chr8g0372251 36.842 114 69 2 3 116 72 182 4.13e-21 87.4
Msa0775600 MtrunA17_Chr5g0431631 49.333 75 38 0 1 75 87 161 4.63e-21 82.8
Msa0775600 MtrunA17_Chr4g0030701 37.069 116 73 0 1 116 70 185 6.22e-21 86.7
Msa0775600 MtrunA17_Chr5g0431601 46.988 83 43 1 1 83 1 82 6.88e-21 80.1
Msa0775600 MtrunA17_Chr4g0058261 38.739 111 66 1 1 109 97 207 7.20e-21 86.7
Msa0775600 MtrunA17_Chr8g0362481 38.095 105 65 0 1 105 1 105 2.05e-20 85.1
Msa0775600 MtrunA17_Chr8g0344151 43.023 86 49 0 1 86 71 156 2.47e-20 85.1
Msa0775600 MtrunA17_Chr4g0011871 34.783 115 75 0 2 116 66 180 2.63e-20 85.1
Msa0775600 MtrunA17_Chr8g0362701 39.048 105 64 0 1 105 70 174 4.76e-20 84.3
Msa0775600 MtrunA17_Chr4g0058281 38.532 109 67 0 1 109 81 189 5.48e-20 84.0
Msa0775600 MtrunA17_Chr4g0058191 39.362 94 57 0 1 94 60 153 6.39e-20 84.0
Msa0775600 MtrunA17_Chr5g0419691 32.759 116 78 0 1 116 65 180 7.04e-20 84.0
Msa0775600 MtrunA17_Chr3g0117321 45.238 84 46 0 1 84 42 125 1.01e-19 78.6
Msa0775600 MtrunA17_Chr8g0344181 41.935 93 54 0 1 93 73 165 1.30e-19 83.2
Msa0775600 MtrunA17_Chr3g0094231 36.190 105 67 0 1 105 71 175 1.54e-19 82.8
Msa0775600 MtrunA17_Chr3g0086691 36.697 109 69 0 1 109 67 175 1.78e-19 82.8
Msa0775600 MtrunA17_Chr4g0061381 36.697 109 65 1 1 105 66 174 2.11e-19 82.4
Msa0775600 MtrunA17_Chr1g0174471 38.235 102 63 0 5 106 89 190 2.17e-19 82.4
Msa0775600 MtrunA17_Chr5g0402971 41.489 94 55 0 2 95 78 171 2.80e-19 81.3
Msa0775600 MtrunA17_Chr2g0314771 31.933 119 79 1 1 119 1 117 3.32e-19 78.2
Msa0775600 MtrunA17_Chr3g0084921 39.048 105 64 0 3 107 72 176 4.23e-19 81.6
Msa0775600 MtrunA17_Chr4g0047641 41.176 85 50 0 1 85 85 169 5.02e-19 81.3
Msa0775600 MtrunA17_Chr3g0086651 36.937 111 64 1 1 105 67 177 5.10e-19 81.3
Msa0775600 MtrunA17_Chr3g0086661 36.937 111 64 1 1 105 67 177 5.41e-19 81.3
Msa0775600 MtrunA17_Chr4g0035921 35.965 114 70 1 3 116 74 184 5.68e-19 81.3
Msa0775600 MtrunA17_Chr5g0405001 34.211 114 72 2 3 116 81 191 6.39e-19 81.3
Msa0775600 MtrunA17_Chr5g0431501 33.621 116 77 0 1 116 69 184 8.31e-19 80.9
Msa0775600 MtrunA17_Chr5g0431461 33.621 116 77 0 1 116 69 184 8.31e-19 80.9
Msa0775600 MtrunA17_Chr4g0047661 42.353 85 49 0 1 85 83 167 1.29e-18 80.1
Msa0775600 MtrunA17_Chr1g0174451 35.714 112 72 0 1 112 72 183 1.37e-18 80.1
Msa0775600 MtrunA17_Chr5g0430051 75.000 44 11 0 1 44 73 116 2.82e-18 74.3
Msa0775600 MtrunA17_Chr2g0314721 36.190 105 67 0 1 105 79 183 3.17e-18 79.0
Msa0775600 MtrunA17_Chr1g0174421 34.579 107 70 0 1 107 83 189 5.88e-18 77.8
Msa0775600 MtrunA17_Chr1g0207841 33.621 116 77 0 1 116 73 188 9.97e-18 77.8
Msa0775600 MtrunA17_Chr4g0011981 34.951 103 67 0 2 104 66 168 1.01e-17 77.8
Msa0775600 MtrunA17_Chr1g0207851 34.862 109 70 1 1 109 73 180 1.42e-17 77.0
Msa0775600 MtrunA17_Chr5g0431511 36.449 107 68 0 3 109 71 177 1.45e-17 77.4
Msa0775600 MtrunA17_Chr4g0011961 33.043 115 77 0 2 116 66 180 2.69e-17 76.6
Msa0775600 MtrunA17_Chr1g0174481 37.736 106 66 0 1 106 69 174 2.82e-17 76.3
Msa0775600 MtrunA17_Chr8g0339311 43.478 92 52 0 2 93 82 173 3.67e-17 76.3
Msa0775600 MtrunA17_Chr4g0030711 32.759 116 78 0 1 116 72 187 4.04e-17 75.9
Msa0775600 MtrunA17_Chr8g0339331 36.264 91 58 0 3 93 78 168 7.07e-17 75.1
Msa0775600 MtrunA17_Chr7g0258211 40.476 84 50 0 1 84 74 157 7.54e-17 75.1
Msa0775600 MtrunA17_Chr4g0011921 33.621 116 77 0 1 116 65 180 8.48e-17 75.1
Msa0775600 MtrunA17_Chr3g0094171 30.275 109 76 0 1 109 75 183 1.79e-16 73.9
Msa0775600 MtrunA17_Chr5g0406651 33.654 104 69 0 1 104 66 169 1.98e-16 73.9
Msa0775600 MtrunA17_Chr2g0304871 35.644 101 65 0 2 102 77 177 2.10e-16 73.9
Msa0775600 MtrunA17_Chr1g0160851 29.412 119 82 1 1 119 69 185 2.14e-16 73.9
Msa0775600 MtrunA17_Chr7g0254641 33.645 107 71 0 3 109 74 180 2.45e-16 73.6
Msa0775600 MtrunA17_Chr4g0011901 33.981 103 68 0 2 104 66 168 2.89e-16 73.6
Msa0775600 MtrunA17_Chr7g0218601 31.304 115 79 0 2 116 71 185 3.58e-16 73.2
Msa0775600 MtrunA17_Chr2g0314731 31.933 119 79 1 1 119 68 184 3.76e-16 73.2
Msa0775600 MtrunA17_Chr4g0046671 41.176 85 50 0 2 86 82 166 5.91e-16 72.0
Msa0775600 MtrunA17_Chr4g0011931 34.615 104 68 0 1 104 65 168 8.40e-16 71.6
Msa0775600 MtrunA17_Chr4g0046631 40.000 85 51 0 2 86 84 168 1.18e-15 71.6
Msa0775600 MtrunA17_Chr6g0451691 35.870 92 59 0 2 93 79 170 1.21e-15 71.6
Msa0775600 MtrunA17_Chr7g0235551 36.559 93 59 0 1 93 69 161 1.37e-15 71.6
Msa0775600 MtrunA17_Chr8g0356001 29.630 108 76 0 1 108 76 183 5.88e-15 69.7
Msa0775600 MtrunA17_Chr1g0205361 28.571 119 83 1 1 119 69 185 8.39e-15 69.3
Msa0775600 MtrunA17_Chr6g0451681 34.375 96 63 0 1 96 98 193 1.09e-14 68.9
Msa0775600 MtrunA17_Chr5g0395331 31.481 108 74 0 1 108 72 179 1.25e-14 68.9
Msa0775600 MtrunA17_Chr8g0355961 28.704 108 77 0 1 108 76 183 1.48e-14 68.6
Msa0775600 MtrunA17_Chr4g0046691 36.047 86 55 0 1 86 76 161 2.68e-14 67.4
Msa0775600 MtrunA17_Chr6g0451751 33.696 92 61 0 2 93 79 170 2.82e-14 67.8
Msa0775600 MtrunA17_Chr4g0008101 32.609 92 62 0 2 93 77 168 3.85e-14 67.4
Msa0775600 MtrunA17_Chr8g0351801 43.421 76 43 0 11 86 80 155 4.40e-14 67.4
Msa0775600 MtrunA17_Chr4g0057001 33.645 107 69 1 3 109 87 191 4.99e-14 67.0
Msa0775600 MtrunA17_Chr4g0039901 31.481 108 74 0 1 108 70 177 5.05e-14 67.0
Msa0775600 MtrunA17_Chr7g0255531 32.456 114 68 1 1 114 63 167 5.58e-14 67.0
Msa0775600 MtrunA17_Chr1g0176831 29.412 119 82 1 1 119 98 214 7.49e-14 66.6
Msa0775600 MtrunA17_Chr4g0056951 34.579 107 68 1 3 109 81 185 7.71e-14 66.6
Msa0775600 MtrunA17_Chr5g0395321 26.852 108 79 0 1 108 77 184 9.51e-14 66.2
Msa0775600 MtrunA17_Chr4g0030741 32.710 107 71 1 1 107 99 204 2.06e-13 65.1
Msa0775600 MtrunA17_Chr1g0205371 26.050 119 86 1 1 119 70 186 3.28e-13 64.7
Msa0775600 MtrunA17_Chr6g0451741 33.696 92 61 0 2 93 98 189 3.82e-13 64.7
Msa0775600 MtrunA17_Chr6g0466941 30.435 92 64 0 2 93 72 163 4.69e-13 64.3
Msa0775600 MtrunA17_Chr7g0218611 32.407 108 73 0 2 109 70 177 5.88e-13 63.9
Msa0775600 MtrunA17_Chr6g0458561 30.769 91 63 0 3 93 94 184 1.11e-12 63.2
Msa0775600 MtrunA17_Chr3g0107561 33.981 103 68 0 3 105 85 187 1.31e-12 62.8
Msa0775600 MtrunA17_Chr8g0344191 36.047 86 55 0 1 86 104 189 2.40e-12 62.4
Msa0775600 MtrunA17_Chr4g0052861 35.106 94 59 2 1 93 67 159 4.54e-12 61.6
Msa0775600 MtrunA17_Chr7g0265611 30.526 95 66 0 3 97 83 177 6.86e-12 60.8
Msa0775600 MtrunA17_Chr5g0395381 26.724 116 84 1 1 116 77 191 6.96e-12 60.8
Msa0775600 MtrunA17_Chr5g0401681 30.303 99 69 0 2 100 68 166 9.12e-12 60.8
Msa0775600 MtrunA17_Chr1g0200961 25.926 108 80 0 1 108 63 170 9.21e-12 60.8
Msa0775600 MtrunA17_Chr1g0197871 28.571 105 75 0 1 105 69 173 1.64e-11 60.1
Msa0775600 MtrunA17_Chr4g0046321 29.907 107 73 2 2 107 72 177 2.40e-11 59.3
Msa0775600 MtrunA17_Chr6g0466901 31.522 92 63 0 2 93 72 163 2.88e-11 59.3
Msa0775600 MtrunA17_Chr4g0039391 34.783 92 59 1 3 93 72 163 2.90e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0775600 AT1G11610.2 42.593 108 62 0 1 108 68 175 1.07e-24 97.8
Msa0775600 AT1G11610.1 42.593 108 62 0 1 108 68 175 1.09e-24 97.8
Msa0775600 AT2G30770.1 40.741 108 64 0 1 108 68 175 3.53e-24 96.3
Msa0775600 AT3G48320.1 41.964 112 63 1 1 112 67 176 3.96e-24 96.3
Msa0775600 AT3G26320.1 40.187 107 64 0 1 107 66 172 2.92e-23 93.6
Msa0775600 AT3G48290.2 39.286 112 66 1 1 112 68 177 7.54e-23 92.8
Msa0775600 AT3G48290.1 39.286 112 66 1 1 112 68 177 9.70e-23 92.4
Msa0775600 AT3G48280.1 40.179 112 65 1 1 112 65 174 2.67e-22 90.9
Msa0775600 AT3G26210.1 41.284 109 64 0 1 109 66 174 1.62e-21 89.0
Msa0775600 AT3G48300.1 39.286 112 66 1 1 112 64 173 1.90e-21 88.6
Msa0775600 AT3G48300.2 39.286 112 66 1 1 112 66 175 1.91e-21 88.6
Msa0775600 AT3G26330.1 38.393 112 69 0 1 112 66 177 2.04e-21 88.6
Msa0775600 AT3G26190.1 38.532 109 67 0 1 109 63 171 4.22e-21 87.8
Msa0775600 AT1G13090.1 40.678 118 63 1 3 120 65 175 4.83e-21 85.9
Msa0775600 AT3G26290.2 37.037 108 68 0 1 108 79 186 8.21e-21 87.0
Msa0775600 AT2G30750.1 43.333 90 51 0 1 90 68 157 8.68e-21 86.7
Msa0775600 AT3G26290.1 37.037 108 68 0 1 108 66 173 9.80e-21 86.7
Msa0775600 AT3G44250.1 44.681 94 52 0 3 96 65 158 1.17e-20 86.3
Msa0775600 AT3G48310.1 38.393 112 67 1 1 112 67 176 1.50e-20 86.3
Msa0775600 AT3G26300.1 38.532 109 67 0 1 109 66 174 1.98e-20 85.9
Msa0775600 AT4G13310.2 39.175 97 59 0 1 97 67 163 2.14e-20 85.1
Msa0775600 AT1G13100.1 40.678 118 63 1 3 120 62 172 2.47e-20 85.5
Msa0775600 AT5G42590.1 40.000 95 57 0 1 95 67 161 2.73e-20 85.5
Msa0775600 AT5G24960.2 36.082 97 62 0 1 97 68 164 2.93e-20 84.7
Msa0775600 AT4G13310.1 35.593 118 74 1 1 116 67 184 3.99e-20 84.7
Msa0775600 AT3G53280.1 43.878 98 55 0 1 98 63 160 4.20e-20 84.7
Msa0775600 AT5G24950.1 33.929 112 74 0 1 112 67 178 4.90e-20 84.7
Msa0775600 AT1G13110.1 39.024 123 68 1 1 123 67 182 5.20e-20 84.7
Msa0775600 AT4G20235.1 39.091 110 64 2 13 119 82 191 5.50e-20 83.6
Msa0775600 AT3G26280.1 38.793 116 71 0 1 116 67 182 5.79e-20 84.3
Msa0775600 AT5G24960.1 36.082 97 62 0 1 97 68 164 6.02e-20 84.3
Msa0775600 AT1G13090.2 40.678 118 63 1 3 120 65 175 6.26e-20 84.3
Msa0775600 AT3G26280.2 38.793 116 71 0 1 116 84 199 6.89e-20 84.3
Msa0775600 AT3G26310.1 34.959 123 74 1 1 123 65 181 7.07e-20 84.3
Msa0775600 AT3G48270.1 38.393 112 67 1 1 112 66 175 8.98e-20 84.0
Msa0775600 AT3G26200.1 42.202 109 63 0 1 109 63 171 9.03e-20 84.0
Msa0775600 AT5G57260.1 34.821 112 73 0 1 112 65 176 1.35e-19 83.6
Msa0775600 AT5G06900.1 35.345 116 75 0 1 116 69 184 1.67e-19 83.2
Msa0775600 AT5G07990.1 38.393 112 65 1 1 108 68 179 3.19e-19 82.4
Msa0775600 AT3G26270.1 37.615 109 68 0 1 109 67 175 3.54e-19 82.0
Msa0775600 AT1G13080.1 43.396 106 60 0 3 108 68 173 1.77e-18 80.1
Msa0775600 AT3G53305.1 38.095 105 65 0 1 105 1 105 2.00e-18 79.3
Msa0775600 AT5G25130.1 35.514 107 69 0 1 107 64 170 3.49e-18 79.3
Msa0775600 AT4G13770.1 34.862 109 71 0 1 109 66 174 4.79e-18 79.0
Msa0775600 AT5G35715.1 38.095 105 65 0 1 105 1 105 5.17e-18 79.0
Msa0775600 AT3G26150.1 38.318 107 66 0 1 107 65 171 5.33e-18 79.0
Msa0775600 AT4G20240.1 33.929 112 74 0 1 112 68 179 5.80e-18 78.6
Msa0775600 AT4G22710.1 34.862 109 71 0 1 109 89 197 8.79e-18 78.2
Msa0775600 AT4G22690.1 34.862 109 71 0 1 109 120 228 8.91e-18 78.2
Msa0775600 AT4G13290.1 35.052 97 63 0 1 97 68 164 1.04e-17 78.2
Msa0775600 AT4G31500.1 33.333 108 72 0 2 109 66 173 1.26e-17 77.8
Msa0775600 AT4G20240.2 33.929 112 74 0 1 112 68 179 1.40e-17 77.4
Msa0775600 AT5G25140.1 32.456 114 77 0 3 116 66 179 1.52e-17 77.4
Msa0775600 AT3G26180.1 38.318 107 66 0 1 107 65 171 2.10e-17 77.0
Msa0775600 AT4G36220.1 34.211 114 72 2 3 116 77 187 2.50e-17 77.0
Msa0775600 AT3G26170.1 38.318 107 66 0 1 107 65 171 2.60e-17 77.0
Msa0775600 AT5G25180.1 33.645 107 71 0 1 107 64 170 4.30e-17 76.3
Msa0775600 AT3G53300.1 37.143 105 66 0 1 105 63 167 5.38e-17 75.9
Msa0775600 AT2G24180.1 40.000 90 54 0 1 90 71 160 8.20e-17 75.5
Msa0775600 AT4G37410.1 29.730 111 77 1 3 112 68 178 3.01e-16 73.9
Msa0775600 AT3G26160.1 34.862 109 71 0 1 109 65 173 3.10e-16 73.9
Msa0775600 AT1G01280.1 32.710 107 72 0 3 109 69 175 3.75e-16 73.6
Msa0775600 AT5G10600.1 39.560 91 55 0 3 93 84 174 4.47e-16 73.6
Msa0775600 AT5G44620.1 36.449 107 68 0 3 109 83 189 1.23e-15 72.0
Msa0775600 AT5G25120.1 33.019 106 71 0 1 106 64 169 1.28e-15 72.0
Msa0775600 AT5G10610.1 32.039 103 70 0 3 105 70 172 1.71e-15 71.6
Msa0775600 AT2G40890.1 33.663 101 67 0 1 101 62 162 2.34e-15 71.2
Msa0775600 AT5G06905.1 35.294 119 75 1 1 119 66 182 2.42e-15 71.2
Msa0775600 AT3G26220.1 39.252 107 65 0 3 109 66 172 2.89e-15 71.2
Msa0775600 AT4G31970.1 38.710 93 57 0 1 93 70 162 3.94e-15 70.9
Msa0775600 AT2G02580.1 39.252 107 65 0 1 107 66 172 7.49e-15 69.7
Msa0775600 AT4G12330.1 35.780 109 68 1 3 109 83 191 1.67e-14 68.9
Msa0775600 AT2G42250.1 37.736 106 66 0 1 106 76 181 2.17e-14 68.6
Msa0775600 AT4G31940.1 39.535 86 52 0 1 86 70 155 2.17e-14 68.6
Msa0775600 AT2G23220.1 33.673 98 65 0 3 100 86 183 2.32e-14 68.6
Msa0775600 AT5G67310.1 34.831 89 57 1 3 90 80 168 2.81e-14 68.2
Msa0775600 AT2G23190.1 34.343 99 65 0 2 100 114 212 1.25e-13 66.2
Msa0775600 AT5G04330.1 36.905 84 53 0 1 84 67 150 2.07e-13 65.9
Msa0775600 AT4G37340.1 32.323 99 67 0 1 99 68 166 2.23e-13 65.9
Msa0775600 AT3G28740.1 32.323 99 67 0 2 100 78 176 2.97e-13 65.5
Msa0775600 AT5G36220.1 33.673 98 64 1 1 97 74 171 7.02e-13 64.3
Msa0775600 AT3G26830.1 33.636 110 73 0 3 112 65 174 1.26e-12 63.5
Msa0775600 AT3G53290.1 35.870 92 59 0 16 107 8 99 1.28e-12 63.5
Msa0775600 AT3G20950.1 31.034 116 80 0 1 116 82 197 1.38e-12 63.5
Msa0775600 AT3G32047.1 30.476 105 73 0 3 107 80 184 1.45e-12 63.5
Msa0775600 AT1G50520.1 36.449 107 61 3 1 102 79 183 2.10e-12 62.8
Msa0775600 AT2G05180.1 33.962 106 70 0 1 106 77 182 2.23e-12 62.8
Msa0775600 AT4G37400.1 29.474 95 66 1 2 95 67 161 2.72e-12 62.4
Msa0775600 AT3G25180.1 28.829 111 79 0 2 112 77 187 3.59e-12 62.4
Msa0775600 AT3G25180.2 28.829 111 79 0 2 112 77 187 3.85e-12 62.0
Msa0775600 AT3G20940.1 31.034 116 80 0 1 116 78 193 4.95e-12 62.0
Msa0775600 AT5G42580.1 31.356 118 77 2 1 116 75 190 7.18e-12 61.2
Msa0775600 AT4G37310.1 34.409 93 60 1 2 93 68 160 9.11e-12 61.2
Msa0775600 AT4G37320.1 27.027 111 80 1 3 112 71 181 9.97e-12 60.8
Msa0775600 AT3G20110.1 34.862 109 67 2 1 107 75 181 1.00e-11 60.8
Msa0775600 AT4G37330.1 27.500 120 86 1 3 121 71 190 1.16e-11 60.8
Msa0775600 AT3G26230.1 33.636 110 73 0 3 112 63 172 1.33e-11 60.5
Msa0775600 AT4G12300.1 36.905 84 53 0 3 86 79 162 1.51e-11 60.5
Msa0775600 AT3G20140.1 32.231 121 72 3 1 119 78 190 2.05e-11 60.1
Msa0775600 AT3G20090.2 29.907 107 75 0 1 107 79 185 3.18e-11 59.7
Msa0775600 AT3G20130.1 30.000 110 77 0 3 112 80 189 3.57e-11 59.3
Msa0775600 AT5G57220.1 26.786 112 80 2 2 112 66 176 3.75e-11 59.3
Msa0775600 AT4G37360.1 29.000 100 71 0 1 100 68 167 3.99e-11 59.3
Msa0775600 AT3G52970.2 26.364 110 79 2 1 109 72 180 4.79e-11 58.9
Msa0775600 AT2G25160.2 32.258 124 79 2 4 123 74 196 5.04e-11 58.9
Msa0775600 AT2G14100.1 28.972 107 76 0 1 107 79 185 5.58e-11 58.9
Msa0775600 AT4G37430.1 30.526 95 65 1 2 95 64 158 5.82e-11 58.9
Msa0775600 AT4G15330.1 33.981 103 68 0 1 103 74 176 7.09e-11 58.5
Msa0775600 AT2G25160.1 32.258 124 79 2 4 123 74 196 7.12e-11 58.5
Msa0775600 AT3G52970.1 28.713 101 70 2 1 100 72 171 8.45e-11 58.2

Find 19 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTGAGTATGGTCCTTAT+TGG 0.276407 5_2:-52583525 Msa0775600:CDS
ACCTAACTCCTCTTGTCTAA+TGG 0.328737 5_2:+52583438 None:intergenic
ACAAGAGGAGTTAGGTGCTT+TGG 0.377792 5_2:-52583431 Msa0775600:CDS
CCTAACTCCTCTTGTCTAAT+GGG 0.378390 5_2:+52583439 None:intergenic
TTTCTCATACTACGCCAATA+AGG 0.393309 5_2:+52583511 None:intergenic
ATCAAAGATGCTTGAACCTT+CGG 0.394613 5_2:+52583577 None:intergenic
CGGGATTTGACCAAGTTGTA+AGG 0.446147 5_2:+52583672 None:intergenic
CCCATTAGACAAGAGGAGTT+AGG 0.485227 5_2:-52583439 Msa0775600:CDS
GCATCTTTGATCATGTCTTA+CGG 0.497791 5_2:-52583565 Msa0775600:CDS
AGGTGCTTTGGTTATGTCGT+TGG 0.507960 5_2:-52583419 Msa0775600:CDS
GAAGATGAAACGATTATAGT+CGG 0.518940 5_2:+52583652 None:intergenic
ATCATGTCTTACGGTTCCAA+AGG 0.530155 5_2:-52583556 Msa0775600:CDS
TCAATCATGTCCTTACAACT+TGG 0.544912 5_2:-52583682 None:intergenic
GTTTGCTCCCATTAGACAAG+AGG 0.549275 5_2:-52583446 Msa0775600:CDS
TCATGTCTTACGGTTCCAAA+GGG 0.555929 5_2:-52583555 Msa0775600:CDS
AAGATGAAACGATTATAGTC+GGG 0.566598 5_2:+52583653 None:intergenic
AAGATGCTTGAACCTTCGGC+CGG 0.567329 5_2:+52583581 None:intergenic
TTGGAGAAAACTGCTATGCT+AGG 0.573451 5_2:-52583400 Msa0775600:CDS
GAAAACTGCTATGCTAGGTG+AGG 0.690846 5_2:-52583395 Msa0775600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGATGAAACGATTATAGTC+GGG + chr5_2:52583350-52583369 None:intergenic 30.0%
GAAGATGAAACGATTATAGT+CGG + chr5_2:52583351-52583370 None:intergenic 30.0%
ATCAAAGATGCTTGAACCTT+CGG + chr5_2:52583426-52583445 None:intergenic 35.0%
GCATCTTTGATCATGTCTTA+CGG - chr5_2:52583435-52583454 Msa0775600:CDS 35.0%
TTTCTGAGTATGGTCCTTAT+TGG - chr5_2:52583475-52583494 Msa0775600:CDS 35.0%
TTTCTCATACTACGCCAATA+AGG + chr5_2:52583492-52583511 None:intergenic 35.0%
!! ATGACATCGTTTTTGCAAGC+CGG - chr5_2:52583400-52583419 Msa0775600:CDS 40.0%
ATCATGTCTTACGGTTCCAA+AGG - chr5_2:52583444-52583463 Msa0775600:CDS 40.0%
TCATGTCTTACGGTTCCAAA+GGG - chr5_2:52583445-52583464 Msa0775600:CDS 40.0%
CTCAGAAAAAGCAAACCCTT+TGG + chr5_2:52583463-52583482 None:intergenic 40.0%
!!! GGGTTTGCTTTTTCTGAGTA+TGG - chr5_2:52583465-52583484 Msa0775600:CDS 40.0%
CCTAACTCCTCTTGTCTAAT+GGG + chr5_2:52583564-52583583 None:intergenic 40.0%
ACCTAACTCCTCTTGTCTAA+TGG + chr5_2:52583565-52583584 None:intergenic 40.0%
TTGGAGAAAACTGCTATGCT+AGG - chr5_2:52583600-52583619 Msa0775600:CDS 40.0%
CGGGATTTGACCAAGTTGTA+AGG + chr5_2:52583331-52583350 None:intergenic 45.0%
GTTTGCTCCCATTAGACAAG+AGG - chr5_2:52583554-52583573 Msa0775600:CDS 45.0%
CCCATTAGACAAGAGGAGTT+AGG - chr5_2:52583561-52583580 Msa0775600:CDS 45.0%
ACAAGAGGAGTTAGGTGCTT+TGG - chr5_2:52583569-52583588 Msa0775600:CDS 45.0%
AGGTGCTTTGGTTATGTCGT+TGG - chr5_2:52583581-52583600 Msa0775600:CDS 45.0%
GAAAACTGCTATGCTAGGTG+AGG - chr5_2:52583605-52583624 Msa0775600:CDS 45.0%
AAGATGCTTGAACCTTCGGC+CGG + chr5_2:52583422-52583441 None:intergenic 50.0%
! CGTTTTTGCAAGCCGGCCGA+AGG - chr5_2:52583407-52583426 Msa0775600:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 52583324 52583698 52583324 ID=Msa0775600;Name=Msa0775600
chr5_2 mRNA 52583324 52583698 52583324 ID=Msa0775600-mRNA-1;Parent=Msa0775600;Name=Msa0775600-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|124
chr5_2 exon 52583324 52583698 52583324 ID=Msa0775600-mRNA-1:exon:15721;Parent=Msa0775600-mRNA-1
chr5_2 CDS 52583324 52583698 52583324 ID=Msa0775600-mRNA-1:cds;Parent=Msa0775600-mRNA-1
Gene Sequence

>Msa0775600

ATGTCCTTACAACTTGGTCAAATCCCGACTATAATCGTTTCATCTTCAGAAGCTGCAGAATCATTTCTCAAAACTCATGACATCGTTTTTGCAAGCCGGCCGAAGGTTCAAGCATCTTTGATCATGTCTTACGGTTCCAAAGGGTTTGCTTTTTCTGAGTATGGTCCTTATTGGCGTAGTATGAGAAAACTTTGCACTTTGAAACTTCTTAGTGCTTCAAAAGTTGAAATGTTTGCTCCCATTAGACAAGAGGAGTTAGGTGCTTTGGTTATGTCGTTGGAGAAAACTGCTATGCTAGGTGAGGTTGTGAATGTTAGTGAAGTTGTAGAGAATCTTAAAAAGACACTAAAAATACTAGTCATTGTTATACCCTAA
Protein sequence

>Msa0775600

MSLQLGQIPTIIVSSSEAAESFLKTHDIVFASRPKVQASLIMSYGSKGFAFSEYGPYWRSMRKLCTLKLLSASKVEMFAPIRQEELGALVMSLEKTAMLGEVVNVSEVVENLKKTLKILVIVIP*