Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0778390 | A0A396HU69 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 4.22e-37 | 127 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0329680 | Msa0778390 | 0.808751 | 2.655744e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0778390 | MtrunA17_Chr5g0434361 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 8.12e-41 | 127 |
| Msa0778390 | MtrunA17_Chr5g0434351 | 84.507 | 71 | 10 | 1 | 1 | 70 | 1 | 71 | 5.77e-37 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 8 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTTGTCGAAAGTGCTTT+TGG | 0.264907 | 5_2:-58825719 | None:intergenic |
| TCCTAGTTTAAACATATTCA+TGG | 0.328729 | 5_2:-58825541 | None:intergenic |
| GCCTTTGTTGAGTTGCTTTG+TGG | 0.344129 | 5_2:+58825584 | Msa0778390:CDS |
| TCCATGAATATGTTTAAACT+AGG | 0.537392 | 5_2:+58825540 | None:intergenic |
| TTGGGCTGCAACATTAGCAA+TGG | 0.555015 | 5_2:-58825700 | None:intergenic |
| ACACTTCACTTCAGCAAGCA+AGG | 0.584455 | 5_2:+58825641 | Msa0778390:CDS |
| TCCACAAAGCAACTCAACAA+AGG | 0.638408 | 5_2:-58825585 | None:intergenic |
| ACTCAACAAAGGCAAGCTTG+AGG | 0.661912 | 5_2:-58825574 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTATCAAAAAGTTGAACTA+TGG | - | chr5_2:58825613-58825632 | None:intergenic | 25.0% |
| !!! | AATTTGTCGAAAGTGCTTTT+GGG | - | chr5_2:58825721-58825740 | None:intergenic | 30.0% |
| !!! | GAATTTGTCGAAAGTGCTTT+TGG | - | chr5_2:58825722-58825741 | None:intergenic | 35.0% |
| TCCACAAAGCAACTCAACAA+AGG | - | chr5_2:58825588-58825607 | None:intergenic | 40.0% | |
| ACTCAACAAAGGCAAGCTTG+AGG | - | chr5_2:58825577-58825596 | None:intergenic | 45.0% | |
| ! | GCCTTTGTTGAGTTGCTTTG+TGG | + | chr5_2:58825584-58825603 | Msa0778390:CDS | 45.0% |
| ACACTTCACTTCAGCAAGCA+AGG | + | chr5_2:58825641-58825660 | Msa0778390:CDS | 45.0% | |
| TTGGGCTGCAACATTAGCAA+TGG | - | chr5_2:58825703-58825722 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 58825543 | 58825755 | 58825543 | ID=Msa0778390;Name=Msa0778390 |
| chr5_2 | mRNA | 58825543 | 58825755 | 58825543 | ID=Msa0778390-mRNA-1;Parent=Msa0778390;Name=Msa0778390-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|70 |
| chr5_2 | exon | 58825543 | 58825755 | 58825543 | ID=Msa0778390-mRNA-1:exon:17294;Parent=Msa0778390-mRNA-1 |
| chr5_2 | CDS | 58825543 | 58825755 | 58825543 | ID=Msa0778390-mRNA-1:cds;Parent=Msa0778390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
