Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0117910 | Msa0781050 | 0.810125 | 1.346412e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAAAAGCTTCTCAACTTC+AGG | 0.326594 | 5_2:-63308499 | Msa0781050:CDS |
| TTTGTTCAAGGATTCTTGTA+AGG | 0.398340 | 5_2:+63308522 | None:intergenic |
| CATCAGCACCTTCCGCTTCA+AGG | 0.409002 | 5_2:-63308432 | Msa0781050:CDS |
| CTCTGGACATACTCATTGAT+TGG | 0.445765 | 5_2:+63309398 | None:intergenic |
| TCCGGATATACTCACAGCTC+TGG | 0.517884 | 5_2:+63309381 | None:intergenic |
| TGATGTTGTCCACAGATACA+AGG | 0.575524 | 5_2:+63309444 | None:intergenic |
| TTCTCAACTTCAGGAAACAA+AGG | 0.579565 | 5_2:-63308490 | Msa0781050:CDS |
| AACTTCAGGAAACAAAGGAC+TGG | 0.603994 | 5_2:-63308485 | Msa0781050:CDS |
| CAAATTAGAAGACCTTGAAG+CGG | 0.623512 | 5_2:+63308420 | None:intergenic |
| ATAATGTGACCTTGTATCTG+TGG | 0.628890 | 5_2:-63309453 | Msa0781050:CDS |
| TTAGAAGACCTTGAAGCGGA+AGG | 0.640761 | 5_2:+63308424 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATGTAAAATGACATAAA+AGG | - | chr5_2:63309053-63309072 | Msa0781050:intron | 15.0% |
| !! | TAAATGTAAAATGACATAAA+AGG | - | chr5_2:63309053-63309072 | Msa0781050:intron | 15.0% |
| !!! | GTTTTGTTACAAGAATTGTA+TGG | - | chr5_2:63308662-63308681 | Msa0781050:intron | 25.0% |
| ! | AGTGAAAGAGATATATGTTA+TGG | - | chr5_2:63308689-63308708 | Msa0781050:intron | 25.0% |
| !!! | NNNNNNNAGTAAGTTGGTAG+CGG | + | chr5_2:63309165-63309184 | None:intergenic | 25.0% |
| !!! | GTTTTGTTACAAGAATTGTA+TGG | - | chr5_2:63308662-63308681 | Msa0781050:intron | 25.0% |
| ! | AGTGAAAGAGATATATGTTA+TGG | - | chr5_2:63308689-63308708 | Msa0781050:intron | 25.0% |
| !!! | NNNNNNNAGTAAGTTGGTAG+CGG | + | chr5_2:63309165-63309184 | None:intergenic | 25.0% |
| ! | ATTGTTGCATAGTTTGAGTT+TGG | - | chr5_2:63308542-63308561 | Msa0781050:intron | 30.0% |
| ! | GATAAGCTAATTGAAGTGTT+TGG | - | chr5_2:63309005-63309024 | Msa0781050:intron | 30.0% |
| ! | GAAGTGTTTGGTAAAATTAG+CGG | - | chr5_2:63309017-63309036 | Msa0781050:intron | 30.0% |
| TTTGTTCAAGGATTCTTGTA+AGG | + | chr5_2:63309377-63309396 | None:intergenic | 30.0% | |
| ! | ATTGTTGCATAGTTTGAGTT+TGG | - | chr5_2:63308542-63308561 | Msa0781050:intron | 30.0% |
| ! | GATAAGCTAATTGAAGTGTT+TGG | - | chr5_2:63309005-63309024 | Msa0781050:intron | 30.0% |
| ! | GAAGTGTTTGGTAAAATTAG+CGG | - | chr5_2:63309017-63309036 | Msa0781050:intron | 30.0% |
| TTTGTTCAAGGATTCTTGTA+AGG | + | chr5_2:63309377-63309396 | None:intergenic | 30.0% | |
| ATAATGTGACCTTGTATCTG+TGG | - | chr5_2:63308443-63308462 | Msa0781050:CDS | 35.0% | |
| AACTATGCAACAATGTCATC+CGG | + | chr5_2:63308536-63308555 | None:intergenic | 35.0% | |
| !! | TGAGTTTGGCAATTTTCAAG+CGG | - | chr5_2:63308556-63308575 | Msa0781050:intron | 35.0% |
| ACGAAACGAAGATTGTAGAA+GGG | + | chr5_2:63308614-63308633 | None:intergenic | 35.0% | |
| TTCGTTTCGTCTTTCATGTT+TGG | - | chr5_2:63308624-63308643 | Msa0781050:intron | 35.0% | |
| !! | TCATGTTTGGTGTATTTCAG+TGG | - | chr5_2:63308637-63308656 | Msa0781050:intron | 35.0% |
| TGCCAATAATGAAGTGATTG+TGG | - | chr5_2:63308810-63308829 | Msa0781050:intron | 35.0% | |
| GCCAATAATGAAGTGATTGT+GGG | - | chr5_2:63308811-63308830 | Msa0781050:intron | 35.0% | |
| AAAAAAGGTGGGCAAGATAT+AGG | + | chr5_2:63308853-63308872 | None:intergenic | 35.0% | |
| AATTGCTCACGAAAAAAGGT+GGG | + | chr5_2:63308864-63308883 | None:intergenic | 35.0% | |
| AAATTGCTCACGAAAAAAGG+TGG | + | chr5_2:63308865-63308884 | None:intergenic | 35.0% | |
| CCTAAATTGCTCACGAAAAA+AGG | + | chr5_2:63308868-63308887 | None:intergenic | 35.0% | |
| !!! | CCTTTTTTCGTGAGCAATTT+AGG | - | chr5_2:63308865-63308884 | Msa0781050:intron | 35.0% |
| GCTCTGTTTGGTAAAAATAG+CGG | - | chr5_2:63308887-63308906 | Msa0781050:intron | 35.0% | |
| TTACGGGGATAACTAACTAA+CGG | + | chr5_2:63309136-63309155 | None:intergenic | 35.0% | |
| ! | GAAGCTTTTGTCTTTGTTCA+AGG | + | chr5_2:63309389-63309408 | None:intergenic | 35.0% |
| TTCTCAACTTCAGGAAACAA+AGG | - | chr5_2:63309406-63309425 | Msa0781050:CDS | 35.0% | |
| ATAATGTGACCTTGTATCTG+TGG | - | chr5_2:63308443-63308462 | Msa0781050:CDS | 35.0% | |
| AACTATGCAACAATGTCATC+CGG | + | chr5_2:63308536-63308555 | None:intergenic | 35.0% | |
| !! | TGAGTTTGGCAATTTTCAAG+CGG | - | chr5_2:63308556-63308575 | Msa0781050:intron | 35.0% |
| ACGAAACGAAGATTGTAGAA+GGG | + | chr5_2:63308614-63308633 | None:intergenic | 35.0% | |
| TTCGTTTCGTCTTTCATGTT+TGG | - | chr5_2:63308624-63308643 | Msa0781050:intron | 35.0% | |
| !! | TCATGTTTGGTGTATTTCAG+TGG | - | chr5_2:63308637-63308656 | Msa0781050:intron | 35.0% |
| TGCCAATAATGAAGTGATTG+TGG | - | chr5_2:63308810-63308829 | Msa0781050:intron | 35.0% | |
| GCCAATAATGAAGTGATTGT+GGG | - | chr5_2:63308811-63308830 | Msa0781050:intron | 35.0% | |
| AAAAAAGGTGGGCAAGATAT+AGG | + | chr5_2:63308853-63308872 | None:intergenic | 35.0% | |
| AATTGCTCACGAAAAAAGGT+GGG | + | chr5_2:63308864-63308883 | None:intergenic | 35.0% | |
| AAATTGCTCACGAAAAAAGG+TGG | + | chr5_2:63308865-63308884 | None:intergenic | 35.0% | |
| CCTAAATTGCTCACGAAAAA+AGG | + | chr5_2:63308868-63308887 | None:intergenic | 35.0% | |
| !!! | CCTTTTTTCGTGAGCAATTT+AGG | - | chr5_2:63308865-63308884 | Msa0781050:intron | 35.0% |
| GCTCTGTTTGGTAAAAATAG+CGG | - | chr5_2:63308887-63308906 | Msa0781050:intron | 35.0% | |
| TTACGGGGATAACTAACTAA+CGG | + | chr5_2:63309136-63309155 | None:intergenic | 35.0% | |
| ! | GAAGCTTTTGTCTTTGTTCA+AGG | + | chr5_2:63309389-63309408 | None:intergenic | 35.0% |
| TTCTCAACTTCAGGAAACAA+AGG | - | chr5_2:63309406-63309425 | Msa0781050:CDS | 35.0% | |
| TGATGTTGTCCACAGATACA+AGG | + | chr5_2:63308455-63308474 | None:intergenic | 40.0% | |
| CTCTGGACATACTCATTGAT+TGG | + | chr5_2:63308501-63308520 | None:intergenic | 40.0% | |
| ACGAAGATTGTAGAAGGGAA+GGG | + | chr5_2:63308609-63308628 | None:intergenic | 40.0% | |
| AACGAAGATTGTAGAAGGGA+AGG | + | chr5_2:63308610-63308629 | None:intergenic | 40.0% | |
| GACGAAACGAAGATTGTAGA+AGG | + | chr5_2:63308615-63308634 | None:intergenic | 40.0% | |
| !! | TGTTTGGTGTATTTCAGTGG+TGG | - | chr5_2:63308640-63308659 | Msa0781050:intron | 40.0% |
| TCAAACACCATGCACATTTG+TGG | - | chr5_2:63308733-63308752 | Msa0781050:intron | 40.0% | |
| CAAACACCATGCACATTTGT+GGG | - | chr5_2:63308734-63308753 | Msa0781050:intron | 40.0% | |
| GCCCACAATCACTTCATTAT+TGG | + | chr5_2:63308815-63308834 | None:intergenic | 40.0% | |
| TGAGCAATTTAGGCTCTGTT+TGG | - | chr5_2:63308875-63308894 | Msa0781050:intron | 40.0% | |
| ATTGCTTGAGCTACGTAAAC+AGG | + | chr5_2:63309353-63309372 | None:intergenic | 40.0% | |
| GACAAAAGCTTCTCAACTTC+AGG | - | chr5_2:63309397-63309416 | Msa0781050:CDS | 40.0% | |
| AACTTCAGGAAACAAAGGAC+TGG | - | chr5_2:63309411-63309430 | Msa0781050:CDS | 40.0% | |
| TGATGTTGTCCACAGATACA+AGG | + | chr5_2:63308455-63308474 | None:intergenic | 40.0% | |
| CTCTGGACATACTCATTGAT+TGG | + | chr5_2:63308501-63308520 | None:intergenic | 40.0% | |
| ACGAAGATTGTAGAAGGGAA+GGG | + | chr5_2:63308609-63308628 | None:intergenic | 40.0% | |
| AACGAAGATTGTAGAAGGGA+AGG | + | chr5_2:63308610-63308629 | None:intergenic | 40.0% | |
| GACGAAACGAAGATTGTAGA+AGG | + | chr5_2:63308615-63308634 | None:intergenic | 40.0% | |
| !! | TGTTTGGTGTATTTCAGTGG+TGG | - | chr5_2:63308640-63308659 | Msa0781050:intron | 40.0% |
| TCAAACACCATGCACATTTG+TGG | - | chr5_2:63308733-63308752 | Msa0781050:intron | 40.0% | |
| CAAACACCATGCACATTTGT+GGG | - | chr5_2:63308734-63308753 | Msa0781050:intron | 40.0% | |
| GCCCACAATCACTTCATTAT+TGG | + | chr5_2:63308815-63308834 | None:intergenic | 40.0% | |
| TGAGCAATTTAGGCTCTGTT+TGG | - | chr5_2:63308875-63308894 | Msa0781050:intron | 40.0% | |
| ATTGCTTGAGCTACGTAAAC+AGG | + | chr5_2:63309353-63309372 | None:intergenic | 40.0% | |
| GACAAAAGCTTCTCAACTTC+AGG | - | chr5_2:63309397-63309416 | Msa0781050:CDS | 40.0% | |
| AACTTCAGGAAACAAAGGAC+TGG | - | chr5_2:63309411-63309430 | Msa0781050:CDS | 40.0% | |
| TCCAGAGCTGTGAGTATATC+CGG | - | chr5_2:63308514-63308533 | Msa0781050:CDS | 45.0% | |
| AGCGGATGATCAAACTTTGC+TGG | - | chr5_2:63308574-63308593 | Msa0781050:intron | 45.0% | |
| AAGATTGTAGAAGGGAAGGG+AGG | + | chr5_2:63308606-63308625 | None:intergenic | 45.0% | |
| GCTAATCCCACAAATGTGCA+TGG | + | chr5_2:63308743-63308762 | None:intergenic | 45.0% | |
| !! | NAGTAAGTTGGTAGCGGATG+AGG | + | chr5_2:63309159-63309178 | None:intergenic | 45.0% |
| TTAGAAGACCTTGAAGCGGA+AGG | + | chr5_2:63309475-63309494 | None:intergenic | 45.0% | |
| TCCAGAGCTGTGAGTATATC+CGG | - | chr5_2:63308514-63308533 | Msa0781050:CDS | 45.0% | |
| AGCGGATGATCAAACTTTGC+TGG | - | chr5_2:63308574-63308593 | Msa0781050:intron | 45.0% | |
| AAGATTGTAGAAGGGAAGGG+AGG | + | chr5_2:63308606-63308625 | None:intergenic | 45.0% | |
| GCTAATCCCACAAATGTGCA+TGG | + | chr5_2:63308743-63308762 | None:intergenic | 45.0% | |
| !! | NAGTAAGTTGGTAGCGGATG+AGG | + | chr5_2:63309159-63309178 | None:intergenic | 45.0% |
| TTAGAAGACCTTGAAGCGGA+AGG | + | chr5_2:63309475-63309494 | None:intergenic | 45.0% | |
| TCCGGATATACTCACAGCTC+TGG | + | chr5_2:63308518-63308537 | None:intergenic | 50.0% | |
| !! | TTGGTAGCGGATGAGGTTAC+GGG | + | chr5_2:63309152-63309171 | None:intergenic | 50.0% |
| !! | GTTGGTAGCGGATGAGGTTA+CGG | + | chr5_2:63309153-63309172 | None:intergenic | 50.0% |
| TCCGGATATACTCACAGCTC+TGG | + | chr5_2:63308518-63308537 | None:intergenic | 50.0% | |
| !! | TTGGTAGCGGATGAGGTTAC+GGG | + | chr5_2:63309152-63309171 | None:intergenic | 50.0% |
| !! | GTTGGTAGCGGATGAGGTTA+CGG | + | chr5_2:63309153-63309172 | None:intergenic | 50.0% |
| !! | TGGTAGCGGATGAGGTTACG+GGG | + | chr5_2:63309151-63309170 | None:intergenic | 55.0% |
| CATCAGCACCTTCCGCTTCA+AGG | - | chr5_2:63309464-63309483 | Msa0781050:CDS | 55.0% | |
| !! | TGGTAGCGGATGAGGTTACG+GGG | + | chr5_2:63309151-63309170 | None:intergenic | 55.0% |
| CATCAGCACCTTCCGCTTCA+AGG | - | chr5_2:63309464-63309483 | Msa0781050:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 63308424 | 63309494 | 63308424 | ID=Msa0781050;Name=Msa0781050 |
| chr5_2 | mRNA | 63308424 | 63309494 | 63308424 | ID=Msa0781050-mRNA-1;Parent=Msa0781050;Name=Msa0781050-mRNA-1;_AED=0.78;_eAED=1.00;_QI=0|0|0|0.5|0|0|2|0|76 |
| chr5_2 | exon | 63309396 | 63309494 | 63309396 | ID=Msa0781050-mRNA-1:exon:18853;Parent=Msa0781050-mRNA-1 |
| chr5_2 | exon | 63308424 | 63308555 | 63308424 | ID=Msa0781050-mRNA-1:exon:18852;Parent=Msa0781050-mRNA-1 |
| chr5_2 | CDS | 63309396 | 63309494 | 63309396 | ID=Msa0781050-mRNA-1:cds;Parent=Msa0781050-mRNA-1 |
| chr5_2 | CDS | 63308424 | 63308555 | 63308424 | ID=Msa0781050-mRNA-1:cds;Parent=Msa0781050-mRNA-1 |
| Gene Sequence |
| Protein sequence |
