Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0117910 | MCI04674.1 | 96.774 | 93 | 2 | 1 | 1 | 93 | 17 | 108 | 2.50e-51 | 168 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0117910 | sp|Q7X9V2|PIE1_ARATH | 83.871 | 93 | 14 | 1 | 1 | 93 | 61 | 152 | 6.99e-37 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0117910 | A0A392NYZ7 | 96.774 | 93 | 2 | 1 | 1 | 93 | 17 | 108 | 1.19e-51 | 168 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003220 | Msa0117910 | 0.823478 | 1.355738e-53 | -8.615850e-47 |
| Msa0010770 | Msa0117910 | 0.800249 | 1.574551e-48 | -8.615850e-47 |
| Msa0012540 | Msa0117910 | 0.805589 | 1.241263e-49 | -8.615850e-47 |
| Msa0027390 | Msa0117910 | 0.831180 | 1.931725e-55 | -8.615850e-47 |
| Msa0031180 | Msa0117910 | 0.806127 | 9.564480e-50 | -8.615850e-47 |
| Msa0032370 | Msa0117910 | 0.801712 | 7.911669e-49 | -8.615850e-47 |
| Msa0037120 | Msa0117910 | 0.808703 | 2.718599e-50 | -8.615850e-47 |
| Msa0054530 | Msa0117910 | 0.809345 | 1.981298e-50 | -8.615850e-47 |
| Msa0064920 | Msa0117910 | 0.813971 | 1.953397e-51 | -8.615850e-47 |
| Msa0068430 | Msa0117910 | 0.805025 | 1.629047e-49 | -8.615850e-47 |
| Msa0070370 | Msa0117910 | 0.819527 | 1.108324e-52 | -8.615850e-47 |
| Msa0078060 | Msa0117910 | 0.809942 | 1.474233e-50 | -8.615850e-47 |
| Msa0078480 | Msa0117910 | 0.817168 | 3.792152e-52 | -8.615850e-47 |
| Msa0084340 | Msa0117910 | 0.809038 | 2.304687e-50 | -8.615850e-47 |
| Msa0091780 | Msa0117910 | 0.804310 | 2.297527e-49 | -8.615850e-47 |
| Msa0117830 | Msa0117910 | 0.815980 | 6.999851e-52 | -8.615850e-47 |
| Msa0117910 | Msa0117920 | 0.838140 | 3.431100e-57 | -8.615850e-47 |
| Msa0117910 | Msa0126230 | 0.866096 | 3.659338e-65 | -8.615850e-47 |
| Msa0117910 | Msa0131290 | 0.800638 | 1.312057e-48 | -8.615850e-47 |
| Msa0117910 | Msa0133820 | 0.803716 | 3.051890e-49 | -8.615850e-47 |
| Msa0117910 | Msa0138950 | 0.810789 | 9.677541e-51 | -8.615850e-47 |
| Msa0117910 | Msa0143850 | 0.810302 | 1.233030e-50 | -8.615850e-47 |
| Msa0117910 | Msa0156230 | 0.802281 | 6.044565e-49 | -8.615850e-47 |
| Msa0117910 | Msa0165300 | 0.830111 | 3.529167e-55 | -8.615850e-47 |
| Msa0117910 | Msa0177170 | 0.815106 | 1.095596e-51 | -8.615850e-47 |
| Msa0117910 | Msa0179000 | 0.807908 | 4.016290e-50 | -8.615850e-47 |
| Msa0117910 | Msa0202830 | 0.800331 | 1.515068e-48 | -8.615850e-47 |
| Msa0117910 | Msa0227230 | 0.811393 | 7.161791e-51 | -8.615850e-47 |
| Msa0117910 | Msa0236770 | 0.808469 | 3.049822e-50 | -8.615850e-47 |
| Msa0117910 | Msa0245850 | 0.802219 | 6.224817e-49 | -8.615850e-47 |
| Msa0117910 | Msa0269520 | 0.812511 | 4.086937e-51 | -8.615850e-47 |
| Msa0117910 | Msa0279390 | 0.835765 | 1.386540e-56 | -8.615850e-47 |
| Msa0117910 | Msa0318880 | 0.832731 | 7.995211e-56 | -8.615850e-47 |
| Msa0117910 | Msa0320800 | 0.805071 | 1.593279e-49 | -8.615850e-47 |
| Msa0117910 | Msa0322590 | 0.809634 | 1.717113e-50 | -8.615850e-47 |
| Msa0117910 | Msa0334040 | 0.803376 | 3.590678e-49 | -8.615850e-47 |
| Msa0117910 | Msa0343300 | 0.837087 | 6.392948e-57 | -8.615850e-47 |
| Msa0117910 | Msa0363400 | 0.804587 | 2.011276e-49 | -8.615850e-47 |
| Msa0117910 | Msa0365370 | 0.813323 | 2.712518e-51 | -8.615850e-47 |
| Msa0117910 | Msa0388220 | 0.808806 | 2.584177e-50 | -8.615850e-47 |
| Msa0117910 | Msa0398240 | 0.807448 | 5.030269e-50 | -8.615850e-47 |
| Msa0117910 | Msa0410300 | 0.801601 | 8.336971e-49 | -8.615850e-47 |
| Msa0117910 | Msa0423840 | 0.830285 | 3.200855e-55 | -8.615850e-47 |
| Msa0117910 | Msa0446900 | 0.821424 | 4.067171e-53 | -8.615850e-47 |
| Msa0117910 | Msa0510290 | 0.825603 | 4.284538e-54 | -8.615850e-47 |
| Msa0117910 | Msa0512870 | 0.810535 | 1.098540e-50 | -8.615850e-47 |
| Msa0117910 | Msa0518960 | 0.800633 | 1.314914e-48 | -8.615850e-47 |
| Msa0117910 | Msa0537360 | 0.809846 | 1.546394e-50 | -8.615850e-47 |
| Msa0117910 | Msa0576680 | 0.821161 | 4.677259e-53 | -8.615850e-47 |
| Msa0117910 | Msa0606740 | 0.808471 | 3.046833e-50 | -8.615850e-47 |
| Msa0117910 | Msa0606930 | 0.808638 | 2.806408e-50 | -8.615850e-47 |
| Msa0117910 | Msa0626220 | 0.810719 | 1.002462e-50 | -8.615850e-47 |
| Msa0117910 | Msa0663250 | 0.824937 | 6.157834e-54 | -8.615850e-47 |
| Msa0117910 | Msa0682550 | 0.806599 | 7.607224e-50 | -8.615850e-47 |
| Msa0117910 | Msa0687450 | 0.843583 | 1.281355e-58 | -8.615850e-47 |
| Msa0117910 | Msa0689420 | 0.812180 | 4.827710e-51 | -8.615850e-47 |
| Msa0117910 | Msa0689760 | 0.816097 | 6.589797e-52 | -8.615850e-47 |
| Msa0117910 | Msa0702570 | 0.808927 | 2.434956e-50 | -8.615850e-47 |
| Msa0117910 | Msa0716860 | 0.804147 | 2.484246e-49 | -8.615850e-47 |
| Msa0117910 | Msa0749400 | 0.851725 | 7.362633e-61 | -8.615850e-47 |
| Msa0117910 | Msa0756530 | 0.826208 | 3.077809e-54 | -8.615850e-47 |
| Msa0117910 | Msa0780280 | 0.811934 | 5.461886e-51 | -8.615850e-47 |
| Msa0117910 | Msa0781050 | 0.810125 | 1.346412e-50 | -8.615850e-47 |
| Msa0117910 | Msa0800700 | 0.831953 | 1.246055e-55 | -8.615850e-47 |
| Msa0117910 | Msa0820890 | 0.816363 | 5.748240e-52 | -8.615850e-47 |
| Msa0117910 | Msa0821800 | 0.802279 | 6.050310e-49 | -8.615850e-47 |
| Msa0117910 | Msa0828920 | 0.809950 | 1.468873e-50 | -8.615850e-47 |
| Msa0117910 | Msa0829190 | 0.800569 | 1.355135e-48 | -8.615850e-47 |
| Msa0117910 | Msa0861830 | 0.810944 | 8.958165e-51 | -8.615850e-47 |
| Msa0117910 | Msa0996020 | 0.836168 | 1.096270e-56 | -8.615850e-47 |
| Msa0117910 | Msa1004420 | 0.805043 | 1.614900e-49 | -8.615850e-47 |
| Msa0117910 | Msa1010070 | 0.838831 | 2.276444e-57 | -8.615850e-47 |
| Msa0117910 | Msa1029260 | 0.802417 | 5.667058e-49 | -8.615850e-47 |
| Msa0117910 | Msa1032170 | 0.801099 | 1.056148e-48 | -8.615850e-47 |
| Msa0117910 | Msa1056460 | 0.808164 | 3.542946e-50 | -8.615850e-47 |
| Msa0117910 | Msa1067140 | 0.814353 | 1.609088e-51 | -8.615850e-47 |
| Msa0117910 | Msa1098890 | 0.800139 | 1.658051e-48 | -8.615850e-47 |
| Msa0117910 | Msa1113590 | 0.810818 | 9.540087e-51 | -8.615850e-47 |
| Msa0117910 | Msa1211340 | 0.810065 | 1.387065e-50 | -8.615850e-47 |
| Msa0117910 | Msa1224830 | 0.814376 | 1.590053e-51 | -8.615850e-47 |
| Msa0117910 | Msa1259640 | 0.817319 | 3.508123e-52 | -8.615850e-47 |
| Msa0117910 | Msa1329790 | 0.815076 | 1.112563e-51 | -8.615850e-47 |
| Msa0117910 | Msa1338250 | 0.853013 | 3.162333e-61 | -8.615850e-47 |
| Msa0117910 | Msa1342270 | 0.801332 | 9.463853e-49 | -8.615850e-47 |
| Msa0117910 | Msa1348360 | 0.831269 | 1.837103e-55 | -8.615850e-47 |
| Msa0117910 | Msa1418260 | 0.814915 | 1.208164e-51 | -8.615850e-47 |
| Msa0117910 | Msa1420250 | 0.815612 | 8.453708e-52 | -8.615850e-47 |
| Msa0117910 | Msa1466590 | 0.834667 | 2.625161e-56 | -8.615850e-47 |
| Msa0117910 | Msa1467000 | 0.808266 | 3.369990e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0117910 | MtrunA17_Chr1g0175191 | 98.925 | 93 | 1 | 0 | 1 | 93 | 48 | 140 | 2.58e-54 | 180 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0117910 | AT3G12810.1 | 83.871 | 93 | 14 | 1 | 1 | 93 | 61 | 152 | 7.11e-38 | 134 |
| Msa0117910 | AT3G12810.2 | 80.645 | 93 | 13 | 2 | 1 | 93 | 61 | 148 | 9.20e-34 | 122 |
Find 13 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTAACATGCCTTCTTTGC+TGG | 0.246470 | 1_3:+40005384 | None:intergenic |
| CTAAGGATGTTAAGAAGTTT+TGG | 0.269445 | 1_3:-40005175 | Msa0117910:CDS |
| AGTTGCACTAAATATTTCTA+AGG | 0.336397 | 1_3:-40005192 | Msa0117910:CDS |
| ACTTCGAGTCAGAGAGGAAA+TGG | 0.417938 | 1_3:-40005441 | Msa0117910:CDS |
| GCTTTAAAAGCCAGCAAAGA+AGG | 0.437549 | 1_3:-40005394 | Msa0117910:CDS |
| GGTTCTTTACAAGCATCAAA+TGG | 0.472530 | 1_3:-40005043 | Msa0117910:CDS |
| GAGGAAATGGAAATTAGCTC+AGG | 0.511266 | 1_3:-40005428 | Msa0117910:CDS |
| GCATGTTAGATCCAGCTACA+AGG | 0.540795 | 1_3:-40005372 | Msa0117910:CDS |
| AAGGGGTGAAAAGAAGATGA+AGG | 0.565671 | 1_3:-40005353 | Msa0117910:intron |
| CATGTTAGATCCAGCTACAA+GGG | 0.572694 | 1_3:-40005371 | Msa0117910:CDS |
| CGCAGGACTTCGAGTCAGAG+AGG | 0.627101 | 1_3:-40005447 | None:intergenic |
| ATGTTAGATCCAGCTACAAG+GGG | 0.672689 | 1_3:-40005370 | Msa0117910:CDS |
| TTGTACAGGAAGAAGAGCAG+CGG | 0.707252 | 1_3:-40005223 | Msa0117910:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTAAAATGATTTGATTGC+TGG | - | chr1_3:40005297-40005316 | Msa0117910:intron | 20.0% |
| !! | AGTTGCACTAAATATTTCTA+AGG | - | chr1_3:40005228-40005247 | Msa0117910:intron | 25.0% |
| ! | ACTTGATGAGAAAAAGAAAA+AGG | - | chr1_3:40005401-40005420 | Msa0117910:CDS | 25.0% |
| ! | CTAAGGATGTTAAGAAGTTT+TGG | - | chr1_3:40005245-40005264 | Msa0117910:intron | 30.0% |
| TTGGACAAAAATAGAAAAGC+TGG | - | chr1_3:40005264-40005283 | Msa0117910:intron | 30.0% | |
| ATGAAATGAAGGACATCAAA+GGG | + | chr1_3:40005340-40005359 | None:intergenic | 30.0% | |
| CACAATAAAGGATGAAATGA+AGG | + | chr1_3:40005351-40005370 | None:intergenic | 30.0% | |
| ! | AAACAGCTTGAGTTTCTTTT+AGG | - | chr1_3:40005432-40005451 | Msa0117910:CDS | 30.0% |
| ! | AACAGCTTGAGTTTCTTTTA+GGG | - | chr1_3:40005433-40005452 | Msa0117910:CDS | 30.0% |
| GATGAAATGAAGGACATCAA+AGG | + | chr1_3:40005341-40005360 | None:intergenic | 35.0% | |
| TCATCCTTTATTGTGTTCCT+AGG | - | chr1_3:40005356-40005375 | Msa0117910:CDS | 35.0% | |
| AGAACCTAGGAACACAATAA+AGG | + | chr1_3:40005363-40005382 | None:intergenic | 35.0% | |
| TTGATGCTTGTAAAGAACCT+AGG | + | chr1_3:40005376-40005395 | None:intergenic | 35.0% | |
| GGTTCTTTACAAGCATCAAA+TGG | - | chr1_3:40005377-40005396 | Msa0117910:CDS | 35.0% | |
| GAGGAAATGGAAATTAGCTC+AGG | - | chr1_3:40004992-40005011 | Msa0117910:CDS | 40.0% | |
| !! | GCTTTAAAAGCCAGCAAAGA+AGG | - | chr1_3:40005026-40005045 | Msa0117910:CDS | 40.0% |
| ATCTAACATGCCTTCTTTGC+TGG | + | chr1_3:40005039-40005058 | None:intergenic | 40.0% | |
| CATGTTAGATCCAGCTACAA+GGG | - | chr1_3:40005049-40005068 | Msa0117910:intron | 40.0% | |
| ATGTTAGATCCAGCTACAAG+GGG | - | chr1_3:40005050-40005069 | Msa0117910:intron | 40.0% | |
| AAGGGGTGAAAAGAAGATGA+AGG | - | chr1_3:40005067-40005086 | Msa0117910:intron | 40.0% | |
| ATGAGAACTGCATCTTGTAC+AGG | - | chr1_3:40005183-40005202 | Msa0117910:CDS | 40.0% | |
| ACTTCGAGTCAGAGAGGAAA+TGG | - | chr1_3:40004979-40004998 | Msa0117910:CDS | 45.0% | |
| GCATGTTAGATCCAGCTACA+AGG | - | chr1_3:40005048-40005067 | Msa0117910:intron | 45.0% | |
| ! | TTCTTTTCACCCCTTGTAGC+TGG | + | chr1_3:40005062-40005081 | None:intergenic | 45.0% |
| TCATCACACAACAGTGCAGT+GGG | + | chr1_3:40005167-40005186 | None:intergenic | 45.0% | |
| TTGTACAGGAAGAAGAGCAG+CGG | - | chr1_3:40005197-40005216 | Msa0117910:CDS | 45.0% | |
| ATCACACAACAGTGCAGTGG+GGG | + | chr1_3:40005165-40005184 | None:intergenic | 50.0% | |
| CATCACACAACAGTGCAGTG+GGG | + | chr1_3:40005166-40005185 | None:intergenic | 50.0% | |
| CTCATCACACAACAGTGCAG+TGG | + | chr1_3:40005168-40005187 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 40004978 | 40005464 | 40004978 | ID=Msa0117910;Name=Msa0117910 |
| chr1_3 | mRNA | 40004978 | 40005464 | 40004978 | ID=Msa0117910-mRNA-1;Parent=Msa0117910;Name=Msa0117910-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|1|0.33|1|1|0.66|3|0|93 |
| chr1_3 | exon | 40005354 | 40005464 | 40005354 | ID=Msa0117910-mRNA-1:exon:12254;Parent=Msa0117910-mRNA-1 |
| chr1_3 | exon | 40005157 | 40005237 | 40005157 | ID=Msa0117910-mRNA-1:exon:12253;Parent=Msa0117910-mRNA-1 |
| chr1_3 | exon | 40004978 | 40005064 | 40004978 | ID=Msa0117910-mRNA-1:exon:12252;Parent=Msa0117910-mRNA-1 |
| chr1_3 | CDS | 40005354 | 40005464 | 40005354 | ID=Msa0117910-mRNA-1:cds;Parent=Msa0117910-mRNA-1 |
| chr1_3 | CDS | 40005157 | 40005237 | 40005157 | ID=Msa0117910-mRNA-1:cds;Parent=Msa0117910-mRNA-1 |
| chr1_3 | CDS | 40004978 | 40005064 | 40004978 | ID=Msa0117910-mRNA-1:cds;Parent=Msa0117910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
