AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0784280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0784280 MtrunA17_Chr5g0437251 97.800 591 13 0 12 602 1 591 0.0 1195
Msa0784280 MtrunA17_Chr5g0430211 48.800 625 268 11 12 603 1 606 0.0 560
Msa0784280 MtrunA17_Chr3g0113651 62.250 400 86 7 27 425 1 336 1.81e-166 484
Msa0784280 MtrunA17_Chr3g0113651 49.007 151 63 4 450 597 298 437 2.59e-26 113
Msa0784280 MtrunA17_Chr1g0187591 44.209 613 242 12 12 602 1 535 9.33e-159 469
Msa0784280 MtrunA17_Chr7g0257831 49.194 496 187 12 41 485 47 528 3.70e-148 445
Msa0784280 MtrunA17_Chr3g0088521 46.926 488 191 12 39 476 43 512 3.01e-130 399
Msa0784280 MtrunA17_Chr3g0105101 44.118 476 157 12 44 512 28 401 2.74e-116 352
Msa0784280 MtrunA17_Chr3g0105131 39.664 595 221 19 12 598 1 465 1.13e-107 333
Msa0784280 MtrunA17_Chr1g0171151 37.452 526 254 13 41 520 31 527 2.25e-104 328
Msa0784280 MtrunA17_Chr3g0092171 42.683 410 186 8 12 396 52 437 3.14e-95 301
Msa0784280 MtrunA17_Chr3g0092221 46.048 291 119 6 144 409 51 328 2.19e-75 246
Msa0784280 MtrunA17_Chr3g0105141 68.217 129 40 1 207 334 15 143 9.18e-62 202
Msa0784280 MtrunA17_Chr3g0105121 38.506 348 147 13 227 570 8 292 1.78e-56 194
Msa0784280 MtrunA17_Chr8g0389561 48.357 213 101 4 224 431 77 285 6.49e-55 191
Msa0784280 MtrunA17_Chr6g0487481 68.519 108 34 0 223 330 98 205 7.30e-52 182
Msa0784280 MtrunA17_Chr3g0135351 52.795 161 61 3 218 377 43 189 2.28e-49 172
Msa0784280 MtrunA17_Chr2g0324781 64.865 111 37 1 228 336 77 187 3.45e-48 174
Msa0784280 MtrunA17_Chr4g0040391 60.748 107 42 0 224 330 56 162 2.56e-46 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0784280 AT5G58620.1 50.871 574 211 13 39 598 12 528 0.0 530
Msa0784280 AT2G40140.2 51.500 600 185 18 12 602 1 503 0.0 524
Msa0784280 AT2G40140.1 51.500 600 185 18 12 602 1 503 0.0 524
Msa0784280 AT3G55980.2 60.766 418 146 6 11 424 67 470 5.00e-170 499
Msa0784280 AT3G55980.1 60.671 417 146 6 12 424 1 403 5.47e-170 496
Msa0784280 AT5G12850.1 46.667 495 197 15 36 485 37 509 1.65e-133 407
Msa0784280 AT2G41900.1 47.346 471 186 11 41 476 48 491 1.23e-132 405
Msa0784280 AT5G07500.1 62.698 126 47 0 205 330 29 154 1.51e-55 188
Msa0784280 AT4G29190.1 46.256 227 110 4 206 431 58 273 6.90e-55 190
Msa0784280 AT2G25900.2 68.224 107 34 0 224 330 86 192 1.69e-51 180
Msa0784280 AT2G25900.1 68.224 107 34 0 224 330 86 192 1.69e-51 180
Msa0784280 AT2G19810.1 63.559 118 43 0 213 330 63 180 6.07e-51 180
Msa0784280 AT1G03790.1 55.263 152 54 3 224 371 78 219 4.74e-49 176
Msa0784280 AT5G44260.1 62.963 108 39 1 224 330 56 163 1.97e-44 163

Find 220 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGAAGGTGGTTCATGTTT+TGG 0.196801 5_2:+68805552 None:intergenic
CTGAGGAAATCCGCTTCTTT+TGG 0.197101 5_2:-68805249 Msa0784280:three_prime_UTR
CTGCCACATGTTTCCACTCT+TGG 0.215837 5_2:+68805940 None:intergenic
ACTCGCAAGAATCCCCTTTC+TGG 0.236566 5_2:+68806248 None:intergenic
CTTATGATTGCTTCCTTGTT+TGG 0.250098 5_2:-68806832 Msa0784280:CDS
GACATAAACAACGGTGTATA+TGG 0.256569 5_2:-68806442 Msa0784280:CDS
CGATTTGGACAACGAATTAA+AGG 0.268967 5_2:+68806600 None:intergenic
TGTAGTTGAGATAGTAAATT+AGG 0.275386 5_2:+68805659 None:intergenic
AAAGAATACTTTCACAATAA+TGG 0.294192 5_2:+68804637 None:intergenic
GCAGCCATGTCAAAGCTTCT+TGG 0.294352 5_2:-68805000 Msa0784280:three_prime_UTR
ATCTGTTGTGTGGGTGTTGA+TGG 0.295601 5_2:+68805620 None:intergenic
GTCGTCCGGCCTTTCAGATT+GGG 0.298046 5_2:-68805324 Msa0784280:CDS
TGTCGTCCGGCCTTTCAGAT+TGG 0.305721 5_2:-68805325 Msa0784280:CDS
GCTCTTCATTGTGCTGTTGC+TGG 0.308167 5_2:-68806730 Msa0784280:CDS
CAACACCTTCCTACGCGATT+TGG 0.321673 5_2:+68806585 None:intergenic
CGGAGTTTGTGGTTCCGATA+TGG 0.322011 5_2:-68806758 Msa0784280:CDS
TACACGGGGCGCAGCTCTTC+GGG 0.323922 5_2:+68806118 None:intergenic
CACGACCCCTTGCGAAACTC+TGG 0.328143 5_2:+68806271 None:intergenic
TTGGGTTAATTCATTGGTTA+AGG 0.328824 5_2:-68805166 Msa0784280:three_prime_UTR
TGCCACATGTTTCCACTCTT+GGG 0.340074 5_2:+68805941 None:intergenic
ATACACGGGGCGCAGCTCTT+CGG 0.343094 5_2:+68806117 None:intergenic
CAGCTGCAGTGATGAATTCT+AGG 0.343853 5_2:-68805448 Msa0784280:CDS
AGAAGTAGATGAGAAGGGTT+TGG 0.348805 5_2:-68806929 Msa0784280:CDS
TGTCTCTTGGGTTAATTCAT+TGG 0.351526 5_2:-68805172 Msa0784280:three_prime_UTR
TCTGAGAATTCCGGTGTATA+TGG 0.353483 5_2:-68805135 Msa0784280:three_prime_UTR
TTTGGCCCATGTTCATGTTT+TGG 0.358068 5_2:+68805570 None:intergenic
CGCCTTCCAACCCTTCCTGC+AGG 0.359580 5_2:-68805349 Msa0784280:CDS
TATATGGATCAGATGAGTTT+AGG 0.370932 5_2:-68806426 Msa0784280:CDS
GGAATGACTTGGAGAAATAT+TGG 0.372737 5_2:-68804820 Msa0784280:three_prime_UTR
TTTGTAACCATGATTGGTTT+TGG 0.378830 5_2:-68807281 None:intergenic
ATTTGAATGAAGAAGGCTTT+TGG 0.383843 5_2:-68806906 Msa0784280:CDS
GACATCAGGCTCAGCATGTT+GGG 0.385432 5_2:+68805191 None:intergenic
CTCCTCCTTGCATTCTCACC+TGG 0.394967 5_2:+68806331 None:intergenic
AGAGTCAAAGAACAAAGTTC+AGG 0.399316 5_2:-68804885 Msa0784280:three_prime_UTR
ATGCTGAGCCTGATGTCTCT+TGG 0.404935 5_2:-68805185 Msa0784280:three_prime_UTR
ATTTCCAACTTCACTAGATC+AGG 0.410017 5_2:-68804841 Msa0784280:three_prime_UTR
TTCTGTTGAATTCGCTGTTA+CGG 0.410177 5_2:+68805732 None:intergenic
CTATACTATATGTAAATTGT+TGG 0.411569 5_2:+68804910 None:intergenic
CCCCACTGGGAAACTCGATT+GGG 0.414208 5_2:-68805298 Msa0784280:CDS
GTCAATTCCACTAGCTGAAT+AGG 0.414997 5_2:+68806060 None:intergenic
GAGGAAGGCATATTGTTTGA+TGG 0.415999 5_2:+68805521 None:intergenic
TGGGATGCAGTTGGTGCTGC+TGG 0.418675 5_2:+68805210 None:intergenic
GTCAAGCTCTTGCTTGATGC+CGG 0.418825 5_2:-68806682 Msa0784280:CDS
TATGGTGAGGGCTTCCTCAC+TGG 0.419808 5_2:+68805497 None:intergenic
CGTCCTTGCAAAGCCTTGTT+CGG 0.424614 5_2:+68806182 None:intergenic
CAGCTGTAAGGGTATTTCCT+TGG 0.426047 5_2:+68806304 None:intergenic
ATTGATGTCAACGGAGTTTG+TGG 0.428144 5_2:-68806769 Msa0784280:CDS
TAAATAAATAAAACTTTGTC+AGG 0.428192 5_2:+68804973 None:intergenic
TCGGGCAACGATATATCAAT+AGG 0.428410 5_2:+68806463 None:intergenic
GTCGAGTATATTCTTTCGAC+AGG 0.429694 5_2:-68806796 Msa0784280:CDS
CTGCAGCTGCCGCATATTCT+CGG 0.430426 5_2:+68805110 None:intergenic
AAGAGGTCATCAAGGTTAGT+TGG 0.431942 5_2:+68805695 None:intergenic
GTGAGGAAGCCCTCACCATA+TGG 0.432783 5_2:-68805494 Msa0784280:CDS
GCACTCTTCAAACGGCTACC+TGG 0.432870 5_2:+68805881 None:intergenic
CATTCCTGATCTAGTGAAGT+TGG 0.437959 5_2:+68804837 None:intergenic
AGCTGTAAGGGTATTTCCTT+GGG 0.442755 5_2:+68806305 None:intergenic
CCACTAGCTGAATAGGACTT+TGG 0.443786 5_2:+68806067 None:intergenic
CCCCTTGCGAAACTCTGGGC+AGG 0.444998 5_2:+68806276 None:intergenic
GATGTTCCATCTGAGAATTC+CGG 0.446112 5_2:-68805144 Msa0784280:three_prime_UTR
AATTCGCTGTTACGGAAAGA+TGG 0.453909 5_2:+68805740 None:intergenic
GGGCAGGGAACACAGCTGTA+AGG 0.457289 5_2:+68806292 None:intergenic
GTTTAGGATGTACAGTTTCA+AGG 0.458638 5_2:-68806410 Msa0784280:CDS
TGCTGAGCCTGATGTCTCTT+GGG 0.461989 5_2:-68805184 Msa0784280:three_prime_UTR
TGGTATTCCAAAACCAATCA+TGG 0.462273 5_2:+68807274 None:intergenic
TATACACCGTTGTTTATGTC+GGG 0.462321 5_2:+68806445 None:intergenic
GGTTTGGATTTGAATGAAGA+AGG 0.464213 5_2:-68806913 Msa0784280:CDS
CACTCAAAGCACTCTTCAAA+CGG 0.468740 5_2:+68805873 None:intergenic
TGAGATGGAATTGCTCGGTC+TGG 0.469920 5_2:-68805834 Msa0784280:CDS
GGCTTCCTCACTGGAGAGGA+AGG 0.472160 5_2:+68805506 None:intergenic
CTACCTGGGAGCTGCAACGA+TGG 0.472506 5_2:+68805896 None:intergenic
CAAGAGAGAAGTAGATGAGA+AGG 0.474046 5_2:-68806935 Msa0784280:CDS
TAAAACTATATTGTTGTCCA+TGG 0.474219 5_2:-68804664 Msa0784280:three_prime_UTR
TGGCGCATCTGCATTGCACT+TGG 0.475619 5_2:+68805590 None:intergenic
TTCTCACCTGGATGAACAAA+GGG 0.476487 5_2:+68806343 None:intergenic
AAGAGAGAAGTAGATGAGAA+GGG 0.478152 5_2:-68806934 Msa0784280:CDS
CGCCCCGTGTATGCCTCCAC+TGG 0.481037 5_2:-68806106 Msa0784280:CDS
AACAAGGAAGCAATCATAAG+AGG 0.482396 5_2:+68806835 None:intergenic
CATGGAGGATAAGCTTCAAA+AGG 0.484482 5_2:-68807016 Msa0784280:CDS
GCTGGCGCAATCAGATCAAC+CGG 0.486158 5_2:+68806622 None:intergenic
CACTCTTCAAACGGCTACCT+GGG 0.486258 5_2:+68805882 None:intergenic
CACCACGGTCAATGAAACTT+TGG 0.491260 5_2:+68805399 None:intergenic
GCAAGGGGTCGTGCCAGAAA+GGG 0.492458 5_2:-68806261 Msa0784280:CDS
GGTGCTGCAACTCACCATCT+TGG 0.497686 5_2:-68805380 Msa0784280:CDS
AGTTGGAAATCTTCAAACTC+TGG 0.501799 5_2:+68804854 None:intergenic
CCTTCCTGCAGGGTGTCGTC+CGG 0.501860 5_2:-68805338 Msa0784280:CDS
AACACCACCCATGTCTCCCT+TGG 0.506352 5_2:-68805978 Msa0784280:CDS
AAACTCGATTGGGGTGTAAA+CGG 0.506355 5_2:-68805288 Msa0784280:exon
ATGAAGAGCAGTGGCCATAT+CGG 0.506470 5_2:+68806744 None:intergenic
TGTGGGTGTTGATGGTTGCA+TGG 0.506859 5_2:+68805628 None:intergenic
AGGCTTTGCAAGGACGAGAC+TGG 0.506964 5_2:-68806175 Msa0784280:CDS
CACTCTAGTGCTTCCAAACA+AGG 0.508719 5_2:+68806819 None:intergenic
TGAAGAGTGCTTTGAGTGCA+AGG 0.510749 5_2:-68805868 Msa0784280:CDS
TTGGCTGCGCTGCTTTGCAA+AGG 0.511458 5_2:+68805418 None:intergenic
CTTCATTGTGCTGTTGCTGG+TGG 0.512690 5_2:-68806727 Msa0784280:CDS
AGCCTTGTTCGGTATTGTGC+GGG 0.515045 5_2:+68806193 None:intergenic
GCCTTCCAACCCTTCCTGCA+GGG 0.517193 5_2:-68805348 Msa0784280:CDS
AGACATCAGGCTCAGCATGT+TGG 0.517932 5_2:+68805190 None:intergenic
TTGTACATCTGCTCCACCCA+TGG 0.518688 5_2:+68805057 None:intergenic
TGATGCTAGTGGAAACAAAC+CGG 0.520501 5_2:-68806641 Msa0784280:CDS
CCCTGCCCAGAGTTTCGCAA+GGG 0.522429 5_2:-68806277 Msa0784280:CDS
TCGAGACGTGCTTCCACCAT+GGG 0.522786 5_2:-68805073 Msa0784280:three_prime_UTR
TTCATCCAGGTGAGAATGCA+AGG 0.523324 5_2:-68806336 Msa0784280:CDS
ATTCGCTGTTACGGAAAGAT+GGG 0.523731 5_2:+68805741 None:intergenic
TCCCTGCCCAGAGTTTCGCA+AGG 0.526817 5_2:-68806278 Msa0784280:CDS
AAGAATTGGATCGAAGAAGA+TGG 0.527469 5_2:-68806875 Msa0784280:CDS
CGACACCCTGCAGGAAGGGT+TGG 0.530365 5_2:+68805343 None:intergenic
GTACAAAGAGCAGGAGCAGA+TGG 0.534247 5_2:-68805040 Msa0784280:three_prime_UTR
CCACCATCGTTGCAGCTCCC+AGG 0.534409 5_2:-68805899 Msa0784280:CDS
TCGTCCGGCCTTTCAGATTG+GGG 0.536919 5_2:-68805323 Msa0784280:CDS
GAATTAACCCAAGAGACATC+AGG 0.537150 5_2:+68805177 None:intergenic
GGAGCAGATGTACAAAGAGC+AGG 0.537466 5_2:-68805049 Msa0784280:three_prime_UTR
ACGACCCCTTGCGAAACTCT+GGG 0.538237 5_2:+68806272 None:intergenic
GAACCAGTGGAGGCATACAC+GGG 0.538742 5_2:+68806103 None:intergenic
CGGACGACACCCTGCAGGAA+GGG 0.538863 5_2:+68805339 None:intergenic
GGGACCCCAATCTGAAAGGC+CGG 0.539316 5_2:+68805319 None:intergenic
CCAAAGTCCTATTCAGCTAG+TGG 0.541639 5_2:-68806067 Msa0784280:CDS
TCAAGGTGAAGCCATGCTCA+AGG 0.542484 5_2:-68806393 Msa0784280:CDS
GAGTGTCCCTTTGTTCATCC+AGG 0.543113 5_2:-68806349 Msa0784280:CDS
ATTTCTGAACCCAAAAGAAG+CGG 0.548536 5_2:+68805239 None:intergenic
GTTGTCCAAATCGCGTAGGA+AGG 0.549554 5_2:-68806590 Msa0784280:CDS
ATTGACCCACTAAAGAAAAG+AGG 0.552979 5_2:+68804749 None:intergenic
GGGAGATGATGAACCTGCCA+AGG 0.553827 5_2:+68805961 None:intergenic
AGAAGATGGAATCTGAAAAG+AGG 0.553960 5_2:-68806861 Msa0784280:CDS
ACCCTGCAGGAAGGGTTGGA+AGG 0.554901 5_2:+68805347 None:intergenic
TGGAGAATGCATCTGTTGTG+TGG 0.555021 5_2:+68805610 None:intergenic
TTGGACAACGAATTAAAGGC+TGG 0.557548 5_2:+68806604 None:intergenic
GGCAGGGAACACAGCTGTAA+GGG 0.559340 5_2:+68806293 None:intergenic
ATATACACCGTTGTTTATGT+CGG 0.561652 5_2:+68806444 None:intergenic
GCTGATGAATCAAATCCATA+TGG 0.562833 5_2:+68805479 None:intergenic
AAGCCTTGTTCGGTATTGTG+CGG 0.563056 5_2:+68806192 None:intergenic
TGAACCAGTGGAGGCATACA+CGG 0.563334 5_2:+68806102 None:intergenic
TTCTTCATCAGACTGTGCCA+TGG 0.563892 5_2:-68807034 Msa0784280:CDS
CATCACTGCAGCTGCTACTG+CGG 0.565349 5_2:+68805457 None:intergenic
AAGGCCGGACGACACCCTGC+AGG 0.568796 5_2:+68805334 None:intergenic
TTGACCCACTAAAGAAAAGA+GGG 0.570494 5_2:+68804750 None:intergenic
GATCTTGAGATGGAATTGCT+CGG 0.572830 5_2:-68805839 Msa0784280:CDS
CCTGGGAGCTGCAACGATGG+TGG 0.572925 5_2:+68805899 None:intergenic
GATGCGCCAAAACATGAACA+TGG 0.573046 5_2:-68805576 Msa0784280:CDS
AGACGTGCTTCCACCATGGG+TGG 0.573955 5_2:-68805070 Msa0784280:three_prime_UTR
CTTGTTTGGAAGCACTAGAG+TGG 0.576155 5_2:-68806818 Msa0784280:CDS
CCCTTGCGAAACTCTGGGCA+GGG 0.576535 5_2:+68806277 None:intergenic
TGAACCTGCCAAGGGAGACA+TGG 0.576779 5_2:+68805970 None:intergenic
AGACAATTCAAGCAAGATTG+AGG 0.577524 5_2:+68806981 None:intergenic
ATGGAGGATAAGCTTCAAAA+GGG 0.579380 5_2:-68807015 Msa0784280:CDS
ATCCCGCACAATACCGAACA+AGG 0.579739 5_2:-68806195 Msa0784280:CDS
AAGACAATCAGCATCTGCAC+CGG 0.580324 5_2:+68806663 None:intergenic
CAAGGGCTTACTCTCACGAT+TGG 0.580426 5_2:-68806375 Msa0784280:CDS
GTCGAGACGTGCTTCCACCA+TGG 0.580527 5_2:-68805074 Msa0784280:three_prime_UTR
TCACTAGATCAGGAATGACT+TGG 0.581956 5_2:-68804831 Msa0784280:three_prime_UTR
ATGGATAACTTGCGCGAAGG+TGG 0.584205 5_2:+68805540 None:intergenic
AAGGAGCGTCCATCTCCCCT+TGG 0.584326 5_2:+68806524 None:intergenic
TGAACTCATCCAAGGGGAGA+TGG 0.584869 5_2:-68806533 Msa0784280:CDS
CAAATCGCGTAGGAAGGTGT+TGG 0.585250 5_2:-68806584 Msa0784280:CDS
GCTGATTGTCTTGATGCTAG+TGG 0.585414 5_2:-68806652 Msa0784280:CDS
CCGGACGACACCCTGCAGGA+AGG 0.586791 5_2:+68805338 None:intergenic
GGAGAATGCATCTGTTGTGT+GGG 0.590736 5_2:+68805611 None:intergenic
GAAGAGTGCTTTGAGTGCAA+GGG 0.591839 5_2:-68805867 Msa0784280:CDS
AATTTACTATCTCAACTACA+TGG 0.592297 5_2:-68805656 Msa0784280:CDS
CAAGGTGAAGCCATGCTCAA+GGG 0.594043 5_2:-68806392 Msa0784280:CDS
CAAGGGGTCGTGCCAGAAAG+GGG 0.599774 5_2:-68806260 Msa0784280:CDS
ATACCGAACAAGGCTTTGCA+AGG 0.600596 5_2:-68806185 Msa0784280:CDS
TAGGATCAGCTGATGCTAAG+AGG 0.602013 5_2:+68805678 None:intergenic
TGCTGCTGCTGTTGACGACG+AGG 0.602651 5_2:+68805806 None:intergenic
GAATCAAATCCATATGGTGA+GGG 0.607337 5_2:+68805485 None:intergenic
AGGATTTGGATTATGTGCAG+TGG 0.608930 5_2:-68807072 Msa0784280:CDS
GTAAGTGCTGAACTCATCCA+AGG 0.609847 5_2:-68806541 Msa0784280:CDS
TGAGAGTAAGCCCTTGAGCA+TGG 0.609898 5_2:+68806382 None:intergenic
CCTGCCCAGAGTTTCGCAAG+GGG 0.612209 5_2:-68806276 Msa0784280:CDS
ATTCTCACCTGGATGAACAA+AGG 0.616516 5_2:+68806342 None:intergenic
TGGATGAACAAAGGGACACT+CGG 0.617153 5_2:+68806351 None:intergenic
GGTTCATCATCTCCCAAGAG+TGG 0.617657 5_2:-68805953 Msa0784280:CDS
CTGCAGGAAGGGTTGGAAGG+CGG 0.621056 5_2:+68805350 None:intergenic
ATTCGTTGTCCAAATCGCGT+AGG 0.621353 5_2:-68806594 Msa0784280:CDS
TCAGCATGTTGGGATGCAGT+TGG 0.624317 5_2:+68805201 None:intergenic
CTGATGCTAAGAGGTCATCA+AGG 0.625709 5_2:+68805687 None:intergenic
TGAATCAAATCCATATGGTG+AGG 0.627563 5_2:+68805484 None:intergenic
AGTTGACATCGGCAAAGATG+AGG 0.628879 5_2:+68806006 None:intergenic
GCTGAATAGGACTTTGGTGA+AGG 0.628923 5_2:+68806073 None:intergenic
TATACACCGGAATTCTCAGA+TGG 0.629638 5_2:+68805138 None:intergenic
AGGCATAGCTGAACCAGTGG+AGG 0.631941 5_2:+68806093 None:intergenic
CCCACTGGGAAACTCGATTG+GGG 0.632471 5_2:-68805297 Msa0784280:CDS
TACATCTGCTCCACCCATGG+TGG 0.632978 5_2:+68805060 None:intergenic
ATGCAAGGAGGAGAGACCCA+AGG 0.635278 5_2:-68806321 Msa0784280:CDS
GGAGATGATGAACCTGCCAA+GGG 0.637245 5_2:+68805962 None:intergenic
GAACCTGCCAAGGGAGACAT+GGG 0.638207 5_2:+68805971 None:intergenic
AGCCAAAGTTTCATTGACCG+TGG 0.638346 5_2:-68805401 Msa0784280:CDS
TATATGGTGCCGAGAATATG+CGG 0.638645 5_2:-68805119 Msa0784280:three_prime_UTR
TGAGGGCTTCCTCACTGGAG+AGG 0.638650 5_2:+68805502 None:intergenic
TTCATCAGACTGTGCCATGG+AGG 0.642039 5_2:-68807031 Msa0784280:CDS
AACAGCACAATGAAGAGCAG+TGG 0.643629 5_2:+68806735 None:intergenic
TAAGTGCTGAACTCATCCAA+GGG 0.645605 5_2:-68806540 Msa0784280:CDS
TTCACAATAATGGATAACCA+TGG 0.649528 5_2:+68804647 None:intergenic
CCCCAATCGAGTTTCCCAGT+GGG 0.652420 5_2:+68805297 None:intergenic
GCTGCAACGATGGTGGAGTG+AGG 0.652628 5_2:+68805906 None:intergenic
ACAGGGAAGATTGATGTCAA+CGG 0.653698 5_2:-68806778 Msa0784280:CDS
AAAACCAAGAAGCTTTGACA+TGG 0.654919 5_2:+68804996 None:intergenic
ATGCGCCAAAACATGAACAT+GGG 0.661793 5_2:-68805575 Msa0784280:CDS
CTCCCAAGAGTGGAAACATG+TGG 0.663242 5_2:-68805943 Msa0784280:CDS
AAGGGATCTCGATCTTGAGA+TGG 0.663328 5_2:-68805849 Msa0784280:CDS
TGAAGGCATAGCTGAACCAG+TGG 0.664738 5_2:+68806090 None:intergenic
GCTGTTACGGAAAGATGGGG+AGG 0.665128 5_2:+68805745 None:intergenic
ACCCCAATCGAGTTTCCCAG+TGG 0.665168 5_2:+68805296 None:intergenic
AAGGCGGAGAAAGTCCAAGA+TGG 0.665195 5_2:+68805366 None:intergenic
CCAATCGAGTTTCCCAGTGG+GGG 0.665291 5_2:+68805299 None:intergenic
GATTCTTGCGAGTATGCACA+TGG 0.665419 5_2:-68806238 Msa0784280:CDS
CCCAATCGAGTTTCCCAGTG+GGG 0.666299 5_2:+68805298 None:intergenic
TTCGCTGTTACGGAAAGATG+GGG 0.672071 5_2:+68805742 None:intergenic
GGTGTTGAAACAGTTGACAT+CGG 0.677946 5_2:+68805995 None:intergenic
GTGACGAGCTGAACAAGCTG+AGG 0.679618 5_2:-68805266 Msa0784280:three_prime_UTR
GATGAGGAGCTAAGAGACAA+CGG 0.680167 5_2:+68806022 None:intergenic
GATGGTGAGTTGCAGCACCA+CGG 0.682458 5_2:+68805384 None:intergenic
ATTCTCGGCACCATATACAC+CGG 0.692075 5_2:+68805125 None:intergenic
TCGAGTATATTCTTTCGACA+GGG 0.693087 5_2:-68806795 Msa0784280:CDS
TTGATGGATAACTTGCGCGA+AGG 0.700054 5_2:+68805537 None:intergenic
AACCAGTGGAGGCATACACG+GGG 0.705968 5_2:+68806104 None:intergenic
AAGTGCTGAACTCATCCAAG+GGG 0.716976 5_2:-68806539 Msa0784280:CDS
ATATGCCTTCCTCTCCAGTG+AGG 0.717918 5_2:-68805511 Msa0784280:CDS
TCGTTGCCCGACATAAACAA+CGG 0.735153 5_2:-68806451 Msa0784280:CDS
CCTGCCAAGGGAGACATGGG+TGG 0.744203 5_2:+68805974 None:intergenic
ATCCAGGTGAGAATGCAAGG+AGG 0.764804 5_2:-68806333 Msa0784280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAGTTGAAAATATTTTTA+TGG - chr5_2:68804684-68804703 Msa0784280:three_prime_UTR 10.0%
!!! TTTTATGGATCTATTATTTT+TGG - chr5_2:68804699-68804718 Msa0784280:three_prime_UTR 15.0%
!!! TTTATGGATCTATTATTTTT+GGG - chr5_2:68804700-68804719 Msa0784280:three_prime_UTR 15.0%
!! TAAATAAATAAAACTTTGTC+AGG + chr5_2:68806952-68806971 None:intergenic 15.0%
!! CTATACTATATGTAAATTGT+TGG + chr5_2:68807015-68807034 None:intergenic 20.0%
!!! GTTGTTGTTTTAGATATTGA+TGG - chr5_2:68804735-68804754 Msa0784280:three_prime_UTR 25.0%
! TGTAGTTGAGATAGTAAATT+AGG + chr5_2:68806266-68806285 None:intergenic 25.0%
! AATTTACTATCTCAACTACA+TGG - chr5_2:68806266-68806285 Msa0784280:CDS 25.0%
! TAAAACTATATTGTTGTCCA+TGG - chr5_2:68807258-68807277 Msa0784280:intron 25.0%
AACTAAACAATACAACACCT+TGG + chr5_2:68804671-68804690 None:intergenic 30.0%
!!! TATTTTTGGGATTTGAAGCA+AGG - chr5_2:68804713-68804732 Msa0784280:three_prime_UTR 30.0%
ATTTAATATTCACACAGCAC+AGG + chr5_2:68804785-68804804 None:intergenic 30.0%
!! AAGTTGTATTGATTATGTGC+TGG - chr5_2:68804817-68804836 Msa0784280:three_prime_UTR 30.0%
! ATTTGAATGAAGAAGGCTTT+TGG - chr5_2:68805016-68805035 Msa0784280:three_prime_UTR 30.0%
! TTTTGGTATGGTAGAAGAAT+TGG - chr5_2:68805033-68805052 Msa0784280:three_prime_UTR 30.0%
!!! CCTTCTTTTTTCAATGAGAA+AGG + chr5_2:68805420-68805439 None:intergenic 30.0%
CCTTTCTCATTGAAAAAAGA+AGG - chr5_2:68805417-68805436 Msa0784280:CDS 30.0%
TTCTCATTGAAAAAAGAAGG+TGG - chr5_2:68805420-68805439 Msa0784280:CDS 30.0%
ATATACACCGTTGTTTATGT+CGG + chr5_2:68805481-68805500 None:intergenic 30.0%
TATATGGATCAGATGAGTTT+AGG - chr5_2:68805496-68805515 Msa0784280:CDS 30.0%
!!! TCTTTTGGGTTCAGAAATAA+TGG - chr5_2:68806688-68806707 Msa0784280:CDS 30.0%
! TTGGGTTAATTCATTGGTTA+AGG - chr5_2:68806756-68806775 Msa0784280:CDS 30.0%
!!! TGATTGGTTTTGGAATACCA+AGG - chr5_2:68804651-68804670 Msa0784280:three_prime_UTR 35.0%
ATGGAGGATAAGCTTCAAAA+GGG - chr5_2:68804907-68804926 Msa0784280:three_prime_UTR 35.0%
AGACAATTCAAGCAAGATTG+AGG + chr5_2:68804944-68804963 None:intergenic 35.0%
AAGAGAGAAGTAGATGAGAA+GGG - chr5_2:68804988-68805007 Msa0784280:three_prime_UTR 35.0%
GGTTTGGATTTGAATGAAGA+AGG - chr5_2:68805009-68805028 Msa0784280:three_prime_UTR 35.0%
! AATGAAGAAGGCTTTTGGTA+TGG - chr5_2:68805021-68805040 Msa0784280:three_prime_UTR 35.0%
AAGAATTGGATCGAAGAAGA+TGG - chr5_2:68805047-68805066 Msa0784280:three_prime_UTR 35.0%
AGAAGATGGAATCTGAAAAG+AGG - chr5_2:68805061-68805080 Msa0784280:three_prime_UTR 35.0%
AACAAGGAAGCAATCATAAG+AGG + chr5_2:68805090-68805109 None:intergenic 35.0%
CTTATGATTGCTTCCTTGTT+TGG - chr5_2:68805090-68805109 Msa0784280:three_prime_UTR 35.0%
TCGAGTATATTCTTTCGACA+GGG - chr5_2:68805127-68805146 Msa0784280:three_prime_UTR 35.0%
! CGATTTGGACAACGAATTAA+AGG + chr5_2:68805325-68805344 None:intergenic 35.0%
TATACACCGTTGTTTATGTC+GGG + chr5_2:68805480-68805499 None:intergenic 35.0%
GACATAAACAACGGTGTATA+TGG - chr5_2:68805480-68805499 Msa0784280:CDS 35.0%
! GTTTAGGATGTACAGTTTCA+AGG - chr5_2:68805512-68805531 Msa0784280:CDS 35.0%
TTCTGTTGAATTCGCTGTTA+CGG + chr5_2:68806193-68806212 None:intergenic 35.0%
GAATCAAATCCATATGGTGA+GGG + chr5_2:68806440-68806459 None:intergenic 35.0%
TGAATCAAATCCATATGGTG+AGG + chr5_2:68806441-68806460 None:intergenic 35.0%
GCTGATGAATCAAATCCATA+TGG + chr5_2:68806446-68806465 None:intergenic 35.0%
ATTTCTGAACCCAAAAGAAG+CGG + chr5_2:68806686-68806705 None:intergenic 35.0%
TGTCTCTTGGGTTAATTCAT+TGG - chr5_2:68806750-68806769 Msa0784280:CDS 35.0%
! AAAACCAAGAAGCTTTGACA+TGG + chr5_2:68806929-68806948 None:intergenic 35.0%
AGAGTCAAAGAACAAAGTTC+AGG - chr5_2:68807037-68807056 Msa0784280:CDS 35.0%
AGTTGGAAATCTTCAAACTC+TGG + chr5_2:68807071-68807090 None:intergenic 35.0%
ATTTCCAACTTCACTAGATC+AGG - chr5_2:68807081-68807100 Msa0784280:intron 35.0%
! GGAATGACTTGGAGAAATAT+TGG - chr5_2:68807102-68807121 Msa0784280:intron 35.0%
! ATTGTCCCTCTTTTCTTTAG+TGG - chr5_2:68807167-68807186 Msa0784280:intron 35.0%
! TTGTCCCTCTTTTCTTTAGT+GGG - chr5_2:68807168-68807187 Msa0784280:intron 35.0%
TTGACCCACTAAAGAAAAGA+GGG + chr5_2:68807175-68807194 None:intergenic 35.0%
ATTGACCCACTAAAGAAAAG+AGG + chr5_2:68807176-68807195 None:intergenic 35.0%
AGGATTTGGATTATGTGCAG+TGG - chr5_2:68804850-68804869 Msa0784280:three_prime_UTR 40.0%
!!! CTTTTGAAGCTTATCCTCCA+TGG + chr5_2:68804908-68804927 None:intergenic 40.0%
CATGGAGGATAAGCTTCAAA+AGG - chr5_2:68804906-68804925 Msa0784280:three_prime_UTR 40.0%
CAAGAGAGAAGTAGATGAGA+AGG - chr5_2:68804987-68805006 Msa0784280:three_prime_UTR 40.0%
AGAAGTAGATGAGAAGGGTT+TGG - chr5_2:68804993-68805012 Msa0784280:three_prime_UTR 40.0%
GTCGAGTATATTCTTTCGAC+AGG - chr5_2:68805126-68805145 Msa0784280:three_prime_UTR 40.0%
ACAGGGAAGATTGATGTCAA+CGG - chr5_2:68805144-68805163 Msa0784280:three_prime_UTR 40.0%
ATTGATGTCAACGGAGTTTG+TGG - chr5_2:68805153-68805172 Msa0784280:three_prime_UTR 40.0%
TGATGCTAGTGGAAACAAAC+CGG - chr5_2:68805281-68805300 Msa0784280:exon 40.0%
TTGGACAACGAATTAAAGGC+TGG + chr5_2:68805321-68805340 None:intergenic 40.0%
!! GTGTTGGAGATGTTTTTGAG+AGG - chr5_2:68805354-68805373 Msa0784280:CDS 40.0%
!! TAAGTGCTGAACTCATCCAA+GGG - chr5_2:68805382-68805401 Msa0784280:CDS 40.0%
TCGGGCAACGATATATCAAT+AGG + chr5_2:68805462-68805481 None:intergenic 40.0%
TTCTCACCTGGATGAACAAA+GGG + chr5_2:68805582-68805601 None:intergenic 40.0%
ATTCTCACCTGGATGAACAA+AGG + chr5_2:68805583-68805602 None:intergenic 40.0%
!! AGCTGTAAGGGTATTTCCTT+GGG + chr5_2:68805620-68805639 None:intergenic 40.0%
GTCAATTCCACTAGCTGAAT+AGG + chr5_2:68805865-68805884 None:intergenic 40.0%
! GGTGTTGAAACAGTTGACAT+CGG + chr5_2:68805930-68805949 None:intergenic 40.0%
! CACTCAAAGCACTCTTCAAA+CGG + chr5_2:68806052-68806071 None:intergenic 40.0%
GATCTTGAGATGGAATTGCT+CGG - chr5_2:68806083-68806102 Msa0784280:CDS 40.0%
ATTCGCTGTTACGGAAAGAT+GGG + chr5_2:68806184-68806203 None:intergenic 40.0%
AATTCGCTGTTACGGAAAGA+TGG + chr5_2:68806185-68806204 None:intergenic 40.0%
AAGAGGTCATCAAGGTTAGT+TGG + chr5_2:68806230-68806249 None:intergenic 40.0%
ATGCGCCAAAACATGAACAT+GGG - chr5_2:68806347-68806366 Msa0784280:CDS 40.0%
! TTTGGCCCATGTTCATGTTT+TGG + chr5_2:68806355-68806374 None:intergenic 40.0%
! GAGGAAGGCATATTGTTTGA+TGG + chr5_2:68806404-68806423 None:intergenic 40.0%
AAACTCGATTGGGGTGTAAA+CGG - chr5_2:68806634-68806653 Msa0784280:CDS 40.0%
! TGAGGAAATCCGCTTCTTTT+GGG - chr5_2:68806674-68806693 Msa0784280:CDS 40.0%
GAATTAACCCAAGAGACATC+AGG + chr5_2:68806748-68806767 None:intergenic 40.0%
GATGTTCCATCTGAGAATTC+CGG - chr5_2:68806778-68806797 Msa0784280:CDS 40.0%
TATACACCGGAATTCTCAGA+TGG + chr5_2:68806787-68806806 None:intergenic 40.0%
TCTGAGAATTCCGGTGTATA+TGG - chr5_2:68806787-68806806 Msa0784280:CDS 40.0%
! TATATGGTGCCGAGAATATG+CGG - chr5_2:68806803-68806822 Msa0784280:CDS 40.0%
CATTCCTGATCTAGTGAAGT+TGG + chr5_2:68807088-68807107 None:intergenic 40.0%
TCACTAGATCAGGAATGACT+TGG - chr5_2:68807091-68807110 Msa0784280:intron 40.0%
! TATGTGCTGGTCTATTGTGC+AGG - chr5_2:68804830-68804849 Msa0784280:three_prime_UTR 45.0%
CTGGTCTATTGTGCAGGATT+TGG - chr5_2:68804836-68804855 Msa0784280:three_prime_UTR 45.0%
TTCTTCATCAGACTGTGCCA+TGG - chr5_2:68804888-68804907 Msa0784280:three_prime_UTR 45.0%
CACTCTAGTGCTTCCAAACA+AGG + chr5_2:68805106-68805125 None:intergenic 45.0%
!! CTTGTTTGGAAGCACTAGAG+TGG - chr5_2:68805104-68805123 Msa0784280:three_prime_UTR 45.0%
ATGAAGAGCAGTGGCCATAT+CGG + chr5_2:68805181-68805200 None:intergenic 45.0%
AACAGCACAATGAAGAGCAG+TGG + chr5_2:68805190-68805209 None:intergenic 45.0%
AAGACAATCAGCATCTGCAC+CGG + chr5_2:68805262-68805281 None:intergenic 45.0%
GCTGATTGTCTTGATGCTAG+TGG - chr5_2:68805270-68805289 Msa0784280:three_prime_UTR 45.0%
ATTCGTTGTCCAAATCGCGT+AGG - chr5_2:68805328-68805347 Msa0784280:CDS 45.0%
!! GGAGATGTTTTTGAGAGGTG+AGG - chr5_2:68805359-68805378 Msa0784280:CDS 45.0%
!! GTAAGTGCTGAACTCATCCA+AGG - chr5_2:68805381-68805400 Msa0784280:CDS 45.0%
!! AAGTGCTGAACTCATCCAAG+GGG - chr5_2:68805383-68805402 Msa0784280:CDS 45.0%
TCGTTGCCCGACATAAACAA+CGG - chr5_2:68805471-68805490 Msa0784280:CDS 45.0%
TGGATGAACAAAGGGACACT+CGG + chr5_2:68805574-68805593 None:intergenic 45.0%
TTCATCCAGGTGAGAATGCA+AGG - chr5_2:68805586-68805605 Msa0784280:CDS 45.0%
!! CAGCTGTAAGGGTATTTCCT+TGG + chr5_2:68805621-68805640 None:intergenic 45.0%
GATTCTTGCGAGTATGCACA+TGG - chr5_2:68805684-68805703 Msa0784280:CDS 45.0%
!!! CACATGGTGTTTTCGAGTCA+TGG - chr5_2:68805700-68805719 Msa0784280:CDS 45.0%
!! AAGCCTTGTTCGGTATTGTG+CGG + chr5_2:68805733-68805752 None:intergenic 45.0%
ATACCGAACAAGGCTTTGCA+AGG - chr5_2:68805737-68805756 Msa0784280:CDS 45.0%
GCTGAATAGGACTTTGGTGA+AGG + chr5_2:68805852-68805871 None:intergenic 45.0%
CCACTAGCTGAATAGGACTT+TGG + chr5_2:68805858-68805877 None:intergenic 45.0%
CCAAAGTCCTATTCAGCTAG+TGG - chr5_2:68805855-68805874 Msa0784280:CDS 45.0%
GATGAGGAGCTAAGAGACAA+CGG + chr5_2:68805903-68805922 None:intergenic 45.0%
AGTTGACATCGGCAAAGATG+AGG + chr5_2:68805919-68805938 None:intergenic 45.0%
TGCCACATGTTTCCACTCTT+GGG + chr5_2:68805984-68806003 None:intergenic 45.0%
!! TGAAGAGTGCTTTGAGTGCA+AGG - chr5_2:68806054-68806073 Msa0784280:CDS 45.0%
!! GAAGAGTGCTTTGAGTGCAA+GGG - chr5_2:68806055-68806074 Msa0784280:CDS 45.0%
AAGGGATCTCGATCTTGAGA+TGG - chr5_2:68806073-68806092 Msa0784280:CDS 45.0%
TTCGCTGTTACGGAAAGATG+GGG + chr5_2:68806183-68806202 None:intergenic 45.0%
CTGATGCTAAGAGGTCATCA+AGG + chr5_2:68806238-68806257 None:intergenic 45.0%
TAGGATCAGCTGATGCTAAG+AGG + chr5_2:68806247-68806266 None:intergenic 45.0%
!! ATCTGTTGTGTGGGTGTTGA+TGG + chr5_2:68806305-68806324 None:intergenic 45.0%
GGAGAATGCATCTGTTGTGT+GGG + chr5_2:68806314-68806333 None:intergenic 45.0%
TGGAGAATGCATCTGTTGTG+TGG + chr5_2:68806315-68806334 None:intergenic 45.0%
GATGCGCCAAAACATGAACA+TGG - chr5_2:68806346-68806365 Msa0784280:CDS 45.0%
! TTGATGGATAACTTGCGCGA+AGG + chr5_2:68806388-68806407 None:intergenic 45.0%
CAGCTGCAGTGATGAATTCT+AGG - chr5_2:68806474-68806493 Msa0784280:CDS 45.0%
AGCCAAAGTTTCATTGACCG+TGG - chr5_2:68806521-68806540 Msa0784280:CDS 45.0%
CACCACGGTCAATGAAACTT+TGG + chr5_2:68806526-68806545 None:intergenic 45.0%
! CTGAGGAAATCCGCTTCTTT+TGG - chr5_2:68806673-68806692 Msa0784280:CDS 45.0%
ATTCTCGGCACCATATACAC+CGG + chr5_2:68806800-68806819 None:intergenic 45.0%
TTCATCAGACTGTGCCATGG+AGG - chr5_2:68804891-68804910 Msa0784280:three_prime_UTR 50.0%
CGGAGTTTGTGGTTCCGATA+TGG - chr5_2:68805164-68805183 Msa0784280:three_prime_UTR 50.0%
! GCTCTTCATTGTGCTGTTGC+TGG - chr5_2:68805192-68805211 Msa0784280:three_prime_UTR 50.0%
! CTTCATTGTGCTGTTGCTGG+TGG - chr5_2:68805195-68805214 Msa0784280:three_prime_UTR 50.0%
!!! TGGTTCTGAGTTTTCGCTCG+AGG - chr5_2:68805215-68805234 Msa0784280:three_prime_UTR 50.0%
GTCAAGCTCTTGCTTGATGC+CGG - chr5_2:68805240-68805259 Msa0784280:three_prime_UTR 50.0%
GTTGTCCAAATCGCGTAGGA+AGG - chr5_2:68805332-68805351 Msa0784280:CDS 50.0%
CAACACCTTCCTACGCGATT+TGG + chr5_2:68805340-68805359 None:intergenic 50.0%
! CAAATCGCGTAGGAAGGTGT+TGG - chr5_2:68805338-68805357 Msa0784280:CDS 50.0%
TGAACTCATCCAAGGGGAGA+TGG - chr5_2:68805389-68805408 Msa0784280:CDS 50.0%
TCAAGGTGAAGCCATGCTCA+AGG - chr5_2:68805529-68805548 Msa0784280:CDS 50.0%
CAAGGTGAAGCCATGCTCAA+GGG - chr5_2:68805530-68805549 Msa0784280:CDS 50.0%
TGAGAGTAAGCCCTTGAGCA+TGG + chr5_2:68805543-68805562 None:intergenic 50.0%
CAAGGGCTTACTCTCACGAT+TGG - chr5_2:68805547-68805566 Msa0784280:CDS 50.0%
GAGTGTCCCTTTGTTCATCC+AGG - chr5_2:68805573-68805592 Msa0784280:CDS 50.0%
ATCCAGGTGAGAATGCAAGG+AGG - chr5_2:68805589-68805608 Msa0784280:CDS 50.0%
ACTCGCAAGAATCCCCTTTC+TGG + chr5_2:68805677-68805696 None:intergenic 50.0%
ATCCCGCACAATACCGAACA+AGG - chr5_2:68805727-68805746 Msa0784280:CDS 50.0%
!! AGCCTTGTTCGGTATTGTGC+GGG + chr5_2:68805732-68805751 None:intergenic 50.0%
!! CGTCCTTGCAAAGCCTTGTT+CGG + chr5_2:68805743-68805762 None:intergenic 50.0%
! TGAACCAGTGGAGGCATACA+CGG + chr5_2:68805823-68805842 None:intergenic 50.0%
! TGAAGGCATAGCTGAACCAG+TGG + chr5_2:68805835-68805854 None:intergenic 50.0%
GGAGATGATGAACCTGCCAA+GGG + chr5_2:68805963-68805982 None:intergenic 50.0%
GGTTCATCATCTCCCAAGAG+TGG - chr5_2:68805969-68805988 Msa0784280:CDS 50.0%
CTCCCAAGAGTGGAAACATG+TGG - chr5_2:68805979-68805998 Msa0784280:CDS 50.0%
CTGCCACATGTTTCCACTCT+TGG + chr5_2:68805985-68806004 None:intergenic 50.0%
CACTCTTCAAACGGCTACCT+GGG + chr5_2:68806043-68806062 None:intergenic 50.0%
TGAGATGGAATTGCTCGGTC+TGG - chr5_2:68806088-68806107 Msa0784280:CDS 50.0%
!! TGTGGGTGTTGATGGTTGCA+TGG + chr5_2:68806297-68806316 None:intergenic 50.0%
! CGCGAAGGTGGTTCATGTTT+TGG + chr5_2:68806373-68806392 None:intergenic 50.0%
! ATGGATAACTTGCGCGAAGG+TGG + chr5_2:68806385-68806404 None:intergenic 50.0%
ATATGCCTTCCTCTCCAGTG+AGG - chr5_2:68806411-68806430 Msa0784280:CDS 50.0%
AAGGCGGAGAAAGTCCAAGA+TGG + chr5_2:68806559-68806578 None:intergenic 50.0%
TCAGCATGTTGGGATGCAGT+TGG + chr5_2:68806724-68806743 None:intergenic 50.0%
GACATCAGGCTCAGCATGTT+GGG + chr5_2:68806734-68806753 None:intergenic 50.0%
AGACATCAGGCTCAGCATGT+TGG + chr5_2:68806735-68806754 None:intergenic 50.0%
ATGCTGAGCCTGATGTCTCT+TGG - chr5_2:68806737-68806756 Msa0784280:CDS 50.0%
TGCTGAGCCTGATGTCTCTT+GGG - chr5_2:68806738-68806757 Msa0784280:CDS 50.0%
TTGTACATCTGCTCCACCCA+TGG + chr5_2:68806868-68806887 None:intergenic 50.0%
GGAGCAGATGTACAAAGAGC+AGG - chr5_2:68806873-68806892 Msa0784280:CDS 50.0%
GTACAAAGAGCAGGAGCAGA+TGG - chr5_2:68806882-68806901 Msa0784280:CDS 50.0%
GCAGCCATGTCAAAGCTTCT+TGG - chr5_2:68806922-68806941 Msa0784280:CDS 50.0%
GCTGGCGCAATCAGATCAAC+CGG + chr5_2:68805303-68805322 None:intergenic 55.0%
CTCCTCCTTGCATTCTCACC+TGG + chr5_2:68805594-68805613 None:intergenic 55.0%
ATGCAAGGAGGAGAGACCCA+AGG - chr5_2:68805601-68805620 Msa0784280:CDS 55.0%
GGCAGGGAACACAGCTGTAA+GGG + chr5_2:68805632-68805651 None:intergenic 55.0%
ACGACCCCTTGCGAAACTCT+GGG + chr5_2:68805653-68805672 None:intergenic 55.0%
AGGCTTTGCAAGGACGAGAC+TGG - chr5_2:68805747-68805766 Msa0784280:CDS 55.0%
! AACCAGTGGAGGCATACACG+GGG + chr5_2:68805821-68805840 None:intergenic 55.0%
! GAACCAGTGGAGGCATACAC+GGG + chr5_2:68805822-68805841 None:intergenic 55.0%
AGGCATAGCTGAACCAGTGG+AGG + chr5_2:68805832-68805851 None:intergenic 55.0%
AACACCACCCATGTCTCCCT+TGG - chr5_2:68805944-68805963 Msa0784280:CDS 55.0%
GAACCTGCCAAGGGAGACAT+GGG + chr5_2:68805954-68805973 None:intergenic 55.0%
TGAACCTGCCAAGGGAGACA+TGG + chr5_2:68805955-68805974 None:intergenic 55.0%
GGGAGATGATGAACCTGCCA+AGG + chr5_2:68805964-68805983 None:intergenic 55.0%
GCACTCTTCAAACGGCTACC+TGG + chr5_2:68806044-68806063 None:intergenic 55.0%
GCTGTTACGGAAAGATGGGG+AGG + chr5_2:68806180-68806199 None:intergenic 55.0%
TGGCGCATCTGCATTGCACT+TGG + chr5_2:68806335-68806354 None:intergenic 55.0%
TATGGTGAGGGCTTCCTCAC+TGG + chr5_2:68806428-68806447 None:intergenic 55.0%
GTGAGGAAGCCCTCACCATA+TGG - chr5_2:68806428-68806447 Msa0784280:CDS 55.0%
CATCACTGCAGCTGCTACTG+CGG + chr5_2:68806468-68806487 None:intergenic 55.0%
! TTGGCTGCGCTGCTTTGCAA+AGG + chr5_2:68806507-68806526 None:intergenic 55.0%
GATGGTGAGTTGCAGCACCA+CGG + chr5_2:68806541-68806560 None:intergenic 55.0%
! GGTGCTGCAACTCACCATCT+TGG - chr5_2:68806542-68806561 Msa0784280:CDS 55.0%
TGTCGTCCGGCCTTTCAGAT+TGG - chr5_2:68806597-68806616 Msa0784280:CDS 55.0%
GTCGTCCGGCCTTTCAGATT+GGG - chr5_2:68806598-68806617 Msa0784280:CDS 55.0%
TCGTCCGGCCTTTCAGATTG+GGG - chr5_2:68806599-68806618 Msa0784280:CDS 55.0%
CCAATCGAGTTTCCCAGTGG+GGG + chr5_2:68806626-68806645 None:intergenic 55.0%
CCCAATCGAGTTTCCCAGTG+GGG + chr5_2:68806627-68806646 None:intergenic 55.0%
CCCCACTGGGAAACTCGATT+GGG - chr5_2:68806624-68806643 Msa0784280:CDS 55.0%
CCCCAATCGAGTTTCCCAGT+GGG + chr5_2:68806628-68806647 None:intergenic 55.0%
CCCACTGGGAAACTCGATTG+GGG - chr5_2:68806625-68806644 Msa0784280:CDS 55.0%
ACCCCAATCGAGTTTCCCAG+TGG + chr5_2:68806629-68806648 None:intergenic 55.0%
GTGACGAGCTGAACAAGCTG+AGG - chr5_2:68806656-68806675 Msa0784280:CDS 55.0%
CTGCAGCTGCCGCATATTCT+CGG + chr5_2:68806815-68806834 None:intergenic 55.0%
TCGAGACGTGCTTCCACCAT+GGG - chr5_2:68806849-68806868 Msa0784280:CDS 55.0%
TACATCTGCTCCACCCATGG+TGG + chr5_2:68806865-68806884 None:intergenic 55.0%
!! AAGGAGCGTCCATCTCCCCT+TGG + chr5_2:68805401-68805420 None:intergenic 60.0%
GGGCAGGGAACACAGCTGTA+AGG + chr5_2:68805633-68805652 None:intergenic 60.0%
TCCCTGCCCAGAGTTTCGCA+AGG - chr5_2:68805644-68805663 Msa0784280:CDS 60.0%
CCCTTGCGAAACTCTGGGCA+GGG + chr5_2:68805648-68805667 None:intergenic 60.0%
CCCTGCCCAGAGTTTCGCAA+GGG - chr5_2:68805645-68805664 Msa0784280:CDS 60.0%
CCTGCCCAGAGTTTCGCAAG+GGG - chr5_2:68805646-68805665 Msa0784280:CDS 60.0%
CACGACCCCTTGCGAAACTC+TGG + chr5_2:68805654-68805673 None:intergenic 60.0%
GCAAGGGGTCGTGCCAGAAA+GGG - chr5_2:68805661-68805680 Msa0784280:CDS 60.0%
CAAGGGGTCGTGCCAGAAAG+GGG - chr5_2:68805662-68805681 Msa0784280:CDS 60.0%
ATACACGGGGCGCAGCTCTT+CGG + chr5_2:68805808-68805827 None:intergenic 60.0%
!! GCTGCAACGATGGTGGAGTG+AGG + chr5_2:68806019-68806038 None:intergenic 60.0%
CTACCTGGGAGCTGCAACGA+TGG + chr5_2:68806029-68806048 None:intergenic 60.0%
TGCTGCTGCTGTTGACGACG+AGG + chr5_2:68806119-68806138 None:intergenic 60.0%
GGCTTCCTCACTGGAGAGGA+AGG + chr5_2:68806419-68806438 None:intergenic 60.0%
TGAGGGCTTCCTCACTGGAG+AGG + chr5_2:68806423-68806442 None:intergenic 60.0%
CTGCAGGAAGGGTTGGAAGG+CGG + chr5_2:68806575-68806594 None:intergenic 60.0%
GCCTTCCAACCCTTCCTGCA+GGG - chr5_2:68806574-68806593 Msa0784280:CDS 60.0%
ACCCTGCAGGAAGGGTTGGA+AGG + chr5_2:68806578-68806597 None:intergenic 60.0%
GGGACCCCAATCTGAAAGGC+CGG + chr5_2:68806606-68806625 None:intergenic 60.0%
GTGGGGGACCCCAATCTGAA+AGG + chr5_2:68806610-68806629 None:intergenic 60.0%
TTCAGATTGGGGTCCCCCAC+TGG - chr5_2:68806610-68806629 Msa0784280:CDS 60.0%
TCAGATTGGGGTCCCCCACT+GGG - chr5_2:68806611-68806630 Msa0784280:CDS 60.0%
CCCCCACTGGGAAACTCGAT+TGG - chr5_2:68806623-68806642 Msa0784280:CDS 60.0%
!! TGGGATGCAGTTGGTGCTGC+TGG + chr5_2:68806715-68806734 None:intergenic 60.0%
GTCGAGACGTGCTTCCACCA+TGG - chr5_2:68806848-68806867 Msa0784280:CDS 60.0%
AGACGTGCTTCCACCATGGG+TGG - chr5_2:68806852-68806871 Msa0784280:CDS 60.0%
CCCCTTGCGAAACTCTGGGC+AGG + chr5_2:68805649-68805668 None:intergenic 65.0%
CGCAAGGGGTCGTGCCAGAA+AGG - chr5_2:68805660-68805679 Msa0784280:CDS 65.0%
TACACGGGGCGCAGCTCTTC+GGG + chr5_2:68805807-68805826 None:intergenic 65.0%
CCTGCCAAGGGAGACATGGG+TGG + chr5_2:68805951-68805970 None:intergenic 65.0%
CCACCCATGTCTCCCTTGGC+AGG - chr5_2:68805948-68805967 Msa0784280:CDS 65.0%
CCTGGGAGCTGCAACGATGG+TGG + chr5_2:68806026-68806045 None:intergenic 65.0%
CCACCATCGTTGCAGCTCCC+AGG - chr5_2:68806023-68806042 Msa0784280:CDS 65.0%
CGCCTTCCAACCCTTCCTGC+AGG - chr5_2:68806573-68806592 Msa0784280:CDS 65.0%
CGACACCCTGCAGGAAGGGT+TGG + chr5_2:68806582-68806601 None:intergenic 65.0%
CGGACGACACCCTGCAGGAA+GGG + chr5_2:68806586-68806605 None:intergenic 65.0%
!! CCTTCCTGCAGGGTGTCGTC+CGG - chr5_2:68806584-68806603 Msa0784280:CDS 65.0%
CGCCCCGTGTATGCCTCCAC+TGG - chr5_2:68805816-68805835 Msa0784280:CDS 70.0%
CCGGACGACACCCTGCAGGA+AGG + chr5_2:68806587-68806606 None:intergenic 70.0%
AAGGCCGGACGACACCCTGC+AGG + chr5_2:68806591-68806610 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 68804650 68807294 68804650 ID=Msa0784280;Name=Msa0784280
chr5_2 mRNA 68804650 68807294 68804650 ID=Msa0784280-mRNA-1;Parent=Msa0784280;Name=Msa0784280-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0.5|0.5|1|0.5|2|642|607
chr5_2 exon 68804650 68807092 68804650 ID=Msa0784280-mRNA-1:exon:20668;Parent=Msa0784280-mRNA-1
chr5_2 exon 68807272 68807294 68807272 ID=Msa0784280-mRNA-1:exon:20667;Parent=Msa0784280-mRNA-1
chr5_2 CDS 68807272 68807294 68807272 ID=Msa0784280-mRNA-1:cds;Parent=Msa0784280-mRNA-1
chr5_2 CDS 68805292 68807092 68805292 ID=Msa0784280-mRNA-1:cds;Parent=Msa0784280-mRNA-1
chr5_2 three_prime_UTR 68804650 68805291 68804650 ID=Msa0784280-mRNA-1:three_prime_utr;Parent=Msa0784280-mRNA-1
Gene Sequence

>Msa0784280

ATGATTGGTTTTGGAATACCAAGGATTTGGATTATGTGCAGTGGTTCAAAGAGTAAACTTTCTTCATCAGACTGTGCCATGGAGGATAAGCTTCAAAAGGGTGTTCACAACTCCTCAATCTTGCTTGAATTGTCTGCATCTGATGATTTTGATGCTTTCAAGAGAGAAGTAGATGAGAAGGGTTTGGATTTGAATGAAGAAGGCTTTTGGTATGGTAGAAGAATTGGATCGAAGAAGATGGAATCTGAAAAGAGGACTCCTCTTATGATTGCTTCCTTGTTTGGAAGCACTAGAGTGGTCGAGTATATTCTTTCGACAGGGAAGATTGATGTCAACGGAGTTTGTGGTTCCGATATGGCCACTGCTCTTCATTGTGCTGTTGCTGGTGGTTCTGAGTTTTCGCTCGAGGTTGTCAAGCTCTTGCTTGATGCCGGTGCAGATGCTGATTGTCTTGATGCTAGTGGAAACAAACCGGTTGATCTGATTGCGCCAGCCTTTAATTCGTTGTCCAAATCGCGTAGGAAGGTGTTGGAGATGTTTTTGAGAGGTGAGGTAAGTGCTGAACTCATCCAAGGGGAGATGGACGCTCCTTTCTCATTGAAAAAAGAAGGTGGTGATAAGAAAGAGTTTCCTATTGATATATCGTTGCCCGACATAAACAACGGTGTATATGGATCAGATGAGTTTAGGATGTACAGTTTCAAGGTGAAGCCATGCTCAAGGGCTTACTCTCACGATTGGACCGAGTGTCCCTTTGTTCATCCAGGTGAGAATGCAAGGAGGAGAGACCCAAGGAAATACCCTTACAGCTGTGTTCCCTGCCCAGAGTTTCGCAAGGGGTCGTGCCAGAAAGGGGATTCTTGCGAGTATGCACATGGTGTTTTCGAGTCATGGCTACATCCCGCACAATACCGAACAAGGCTTTGCAAGGACGAGACTGGCTGCAATAGAAAAGTCTGCTTCTTTGCTCACAGACCCGAAGAGCTGCGCCCCGTGTATGCCTCCACTGGTTCAGCTATGCCTTCACCAAAGTCCTATTCAGCTAGTGGAATTGACATGACATCGATGAGTCCGTTGTCTCTTAGCTCCTCATCTTTGCCGATGTCAACTGTTTCAACACCACCCATGTCTCCCTTGGCAGGTTCATCATCTCCCAAGAGTGGAAACATGTGGCAGAACAAATTAAACCTCACTCCACCATCGTTGCAGCTCCCAGGTAGCCGTTTGAAGAGTGCTTTGAGTGCAAGGGATCTCGATCTTGAGATGGAATTGCTCGGTCTGGATAGTCCTCGTCGTCAACAGCAGCAGCAACAACAACAACAATTGATTGAAGAGATTGCTAGAATCTCCTCCCCATCTTTCCGTAACAGCGAATTCAACAGAATTGCTGATTTGAATCCAACTAACCTTGATGACCTCTTAGCATCAGCTGATCCTAATTTACTATCTCAACTACATGGACTTTCCATGCAACCATCAACACCCACACAACAGATGCATTCTCCAAGTGCAATGCAGATGCGCCAAAACATGAACATGGGCCAAAACATGAACCACCTTCGCGCAAGTTATCCATCAAACAATATGCCTTCCTCTCCAGTGAGGAAGCCCTCACCATATGGATTTGATTCATCAGCCGCAGTAGCAGCTGCAGTGATGAATTCTAGGTCTGCTGCCTTTGCAAAGCAGCGCAGCCAAAGTTTCATTGACCGTGGTGCTGCAACTCACCATCTTGGACTTTCTCCGCCTTCCAACCCTTCCTGCAGGGTGTCGTCCGGCCTTTCAGATTGGGGTCCCCCACTGGGAAACTCGATTGGGGTGTAA
Protein sequence

>Msa0784280

MIGFGIPRIWIMCSGSKSKLSSSDCAMEDKLQKGVHNSSILLELSASDDFDAFKREVDEKGLDLNEEGFWYGRRIGSKKMESEKRTPLMIASLFGSTRVVEYILSTGKIDVNGVCGSDMATALHCAVAGGSEFSLEVVKLLLDAGADADCLDASGNKPVDLIAPAFNSLSKSRRKVLEMFLRGEVSAELIQGEMDAPFSLKKEGGDKKEFPIDISLPDINNGVYGSDEFRMYSFKVKPCSRAYSHDWTECPFVHPGENARRRDPRKYPYSCVPCPEFRKGSCQKGDSCEYAHGVFESWLHPAQYRTRLCKDETGCNRKVCFFAHRPEELRPVYASTGSAMPSPKSYSASGIDMTSMSPLSLSSSSLPMSTVSTPPMSPLAGSSSPKSGNMWQNKLNLTPPSLQLPGSRLKSALSARDLDLEMELLGLDSPRRQQQQQQQQQLIEEIARISSPSFRNSEFNRIADLNPTNLDDLLASADPNLLSQLHGLSMQPSTPTQQMHSPSAMQMRQNMNMGQNMNHLRASYPSNNMPSSPVRKPSPYGFDSSAAVAAAVMNSRSAAFAKQRSQSFIDRGAATHHLGLSPPSNPSCRVSSGLSDWGPPLGNSIGV*