Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0787220 | sp|P24076|BGIA_MOMCH | 66.176 | 68 | 23 | 0 | 3 | 70 | 1 | 68 | 5.01e-25 | 90.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0787220 | G7KCZ8 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 4.47e-39 | 132 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0077370 | Msa0787220 | 0.800476 | 1.415732e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0787220 | MtrunA17_Chr5g0442291 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 8.61e-43 | 132 |
| Msa0787220 | MtrunA17_Chr1g0187221 | 86.765 | 68 | 9 | 0 | 3 | 70 | 5 | 72 | 2.65e-38 | 121 |
| Msa0787220 | MtrunA17_Chr1g0187161 | 81.429 | 70 | 13 | 0 | 1 | 70 | 1 | 70 | 2.60e-37 | 119 |
| Msa0787220 | MtrunA17_Chr1g0187201 | 79.412 | 68 | 14 | 0 | 3 | 70 | 4 | 71 | 8.39e-33 | 107 |
| Msa0787220 | MtrunA17_Chr5g0419531 | 62.121 | 66 | 25 | 0 | 5 | 70 | 4 | 69 | 5.11e-24 | 85.5 |
| Msa0787220 | MtrunA17_Chr1g0184411 | 58.571 | 70 | 29 | 0 | 1 | 70 | 1 | 70 | 1.47e-23 | 84.3 |
| Msa0787220 | MtrunA17_Chr1g0184401 | 57.576 | 66 | 28 | 0 | 5 | 70 | 8 | 73 | 5.38e-22 | 80.5 |
| Msa0787220 | MtrunA17_Chr3g0085011 | 49.206 | 63 | 32 | 0 | 8 | 70 | 36 | 98 | 1.55e-13 | 59.7 |
| Msa0787220 | MtrunA17_Chr3g0084931 | 47.619 | 63 | 33 | 0 | 8 | 70 | 19 | 81 | 1.15e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0787220 | AT2G38870.1 | 60.000 | 70 | 28 | 0 | 1 | 70 | 1 | 70 | 6.93e-24 | 85.5 |
| Msa0787220 | AT5G43580.1 | 54.167 | 72 | 31 | 1 | 1 | 70 | 28 | 99 | 2.06e-19 | 75.1 |
| Msa0787220 | AT2G38900.2 | 50.000 | 64 | 32 | 0 | 7 | 70 | 25 | 88 | 1.29e-15 | 65.1 |
| Msa0787220 | AT2G38900.1 | 50.000 | 64 | 32 | 0 | 7 | 70 | 10 | 73 | 1.33e-15 | 64.7 |
| Msa0787220 | AT3G46860.1 | 46.032 | 63 | 34 | 0 | 8 | 70 | 23 | 85 | 1.84e-13 | 59.7 |
| Msa0787220 | AT5G43570.1 | 45.946 | 74 | 34 | 1 | 1 | 68 | 1 | 74 | 6.83e-13 | 58.5 |
| Msa0787220 | AT5G43570.2 | 49.180 | 61 | 31 | 0 | 8 | 68 | 24 | 84 | 7.01e-12 | 56.2 |
Find 35 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCCAAGTTATAAATTTA+AGG | 0.116946 | 5_2:+72863205 | Msa0787220:three_prime_UTR |
| GTCTATCAGGTTCCAGTAAT+TGG | 0.190800 | 5_2:+72863135 | Msa0787220:CDS |
| CTGTGACAGGGTTAGGGTTT+GGG | 0.262714 | 5_2:+72863095 | Msa0787220:CDS |
| GACACTTTCTATCCAATTAC+TGG | 0.267846 | 5_2:-72863147 | None:intergenic |
| ACTTCCGCTGTGACAGGGTT+AGG | 0.276358 | 5_2:+72863088 | Msa0787220:CDS |
| TAAAGATGGAATTGTCTATC+AGG | 0.346883 | 5_2:+72863122 | Msa0787220:CDS |
| GCTGTGACAGGGTTAGGGTT+TGG | 0.358066 | 5_2:+72863094 | Msa0787220:CDS |
| ATAAATTTAAGGTTTCAATA+AGG | 0.380429 | 5_2:+72863216 | Msa0787220:three_prime_UTR |
| AACATATAGTAGTATACAAT+TGG | 0.391590 | 5_2:-72863252 | None:intergenic |
| AGGGTTTGGGTTGATAAAGA+TGG | 0.412719 | 5_2:+72863108 | Msa0787220:CDS |
| GAAACCTTAAATTTATAACT+TGG | 0.420821 | 5_2:-72863209 | None:intergenic |
| CCGCCGACTTCCGCTGTGAC+AGG | 0.421191 | 5_2:+72863082 | Msa0787220:CDS |
| CTTCCGCTGTGACAGGGTTA+GGG | 0.422816 | 5_2:+72863089 | Msa0787220:CDS |
| TACATATTGAAGGATCAAAA+AGG | 0.429594 | 5_2:+72862819 | Msa0787220:five_prime_UTR |
| CCTTCCACTCCAACCAACTC+TGG | 0.454716 | 5_2:-72862982 | None:intergenic |
| GAAATAGTCATACATATTGA+AGG | 0.490665 | 5_2:+72862809 | Msa0787220:five_prime_UTR |
| CCAGAGTTGGTTGGAGTGGA+AGG | 0.515293 | 5_2:+72862982 | Msa0787220:CDS |
| GTTGGTTGGAGTGGAAGGAA+AGG | 0.527079 | 5_2:+72862987 | Msa0787220:CDS |
| GTGTCATATATATATTTCCT+TGG | 0.538122 | 5_2:-72863349 | None:intergenic |
| AATGCCGTCATTGTGCCAAA+AGG | 0.547092 | 5_2:+72863048 | Msa0787220:CDS |
| TATGGTCAGGTAAGAGCTCA+TGG | 0.552544 | 5_2:+72862959 | Msa0787220:intron |
| CGCCGACTTCCGCTGTGACA+GGG | 0.553611 | 5_2:+72863083 | Msa0787220:CDS |
| GCGGTGACAACAGATCCTTT+TGG | 0.561608 | 5_2:-72863063 | None:intergenic |
| ATGGCCAGAGTTGGTTGGAG+TGG | 0.564909 | 5_2:+72862978 | Msa0787220:CDS |
| AATTGAGAGGGAGAATCCAT+TGG | 0.570404 | 5_2:+72863023 | Msa0787220:CDS |
| AACCCTGTCACAGCGGAAGT+CGG | 0.575336 | 5_2:-72863085 | None:intergenic |
| AGCTCATGGCCAGAGTTGGT+TGG | 0.578782 | 5_2:+72862973 | Msa0787220:CDS |
| TAAGAGCTCATGGCCAGAGT+TGG | 0.585826 | 5_2:+72862969 | Msa0787220:CDS |
| AGCAGAAGCTACAATTGAGA+GGG | 0.593359 | 5_2:+72863011 | Msa0787220:CDS |
| TAGCAGAAGCTACAATTGAG+AGG | 0.609404 | 5_2:+72863010 | Msa0787220:CDS |
| ACAATGACGGCATTCACCAA+TGG | 0.628966 | 5_2:-72863039 | None:intergenic |
| TAATTGGATAGAAAGTGTCA+TGG | 0.646984 | 5_2:+72863151 | Msa0787220:exon |
| CCTGTCACAGCGGAAGTCGG+CGG | 0.661655 | 5_2:-72863082 | None:intergenic |
| AAAATGTCTGATGAATGCAA+AGG | 0.675933 | 5_2:+72862850 | Msa0787220:exon |
| AAACCCTAACCCTGTCACAG+CGG | 0.735906 | 5_2:-72863092 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGAATTAATCAATATTGTA+TGG | + | chr5_2:72862941-72862960 | Msa0787220:intron | 15.0% |
| !!! | ATAAATTTAAGGTTTCAATA+AGG | + | chr5_2:72863216-72863235 | Msa0787220:three_prime_UTR | 15.0% |
| !! | GAAACCTTAAATTTATAACT+TGG | - | chr5_2:72863212-72863231 | None:intergenic | 20.0% |
| !! | AACATATAGTAGTATACAAT+TGG | - | chr5_2:72863255-72863274 | None:intergenic | 20.0% |
| ! | GAAATAGTCATACATATTGA+AGG | + | chr5_2:72862809-72862828 | Msa0787220:five_prime_UTR | 25.0% |
| ! | TACATATTGAAGGATCAAAA+AGG | + | chr5_2:72862819-72862838 | Msa0787220:five_prime_UTR | 25.0% |
| ! | TTAATCAATATTGTATGGTC+AGG | + | chr5_2:72862946-72862965 | Msa0787220:intron | 25.0% |
| ! | TGCTCCAAGTTATAAATTTA+AGG | + | chr5_2:72863205-72863224 | Msa0787220:three_prime_UTR | 25.0% |
| ! | GTGTCATATATATATTTCCT+TGG | - | chr5_2:72863352-72863371 | None:intergenic | 25.0% |
| AAAATGTCTGATGAATGCAA+AGG | + | chr5_2:72862850-72862869 | Msa0787220:exon | 30.0% | |
| TAAAGATGGAATTGTCTATC+AGG | + | chr5_2:72863122-72863141 | Msa0787220:CDS | 30.0% | |
| !! | TAATTGGATAGAAAGTGTCA+TGG | + | chr5_2:72863151-72863170 | Msa0787220:exon | 30.0% |
| GACACTTTCTATCCAATTAC+TGG | - | chr5_2:72863150-72863169 | None:intergenic | 35.0% | |
| ! | GTTTTTCATTTTCTCTGCCA+AGG | + | chr5_2:72863332-72863351 | Msa0787220:three_prime_UTR | 35.0% |
| GTCTGATGAATGCAAAGGTA+AGG | + | chr5_2:72862855-72862874 | Msa0787220:intron | 40.0% | |
| TAGCAGAAGCTACAATTGAG+AGG | + | chr5_2:72863010-72863029 | Msa0787220:CDS | 40.0% | |
| AGCAGAAGCTACAATTGAGA+GGG | + | chr5_2:72863011-72863030 | Msa0787220:CDS | 40.0% | |
| AATTGAGAGGGAGAATCCAT+TGG | + | chr5_2:72863023-72863042 | Msa0787220:CDS | 40.0% | |
| ! | AGATCCTTTTGGCACAATGA+CGG | - | chr5_2:72863055-72863074 | None:intergenic | 40.0% |
| !! | AGGGTTTGGGTTGATAAAGA+TGG | + | chr5_2:72863108-72863127 | Msa0787220:CDS | 40.0% |
| GTCTATCAGGTTCCAGTAAT+TGG | + | chr5_2:72863135-72863154 | Msa0787220:CDS | 40.0% | |
| TATGGTCAGGTAAGAGCTCA+TGG | + | chr5_2:72862959-72862978 | Msa0787220:intron | 45.0% | |
| ACAATGACGGCATTCACCAA+TGG | - | chr5_2:72863042-72863061 | None:intergenic | 45.0% | |
| AATGCCGTCATTGTGCCAAA+AGG | + | chr5_2:72863048-72863067 | Msa0787220:CDS | 45.0% | |
| !! | TATAATATGTTTAAATTAAA+TGG | + | chr5_2:72862889-72862908 | Msa0787220:intron | 5.0% |
| !!! | AAATTTTAATTATAATTTGA+TGG | + | chr5_2:72862918-72862937 | Msa0787220:intron | 5.0% |
| TAAGAGCTCATGGCCAGAGT+TGG | + | chr5_2:72862969-72862988 | Msa0787220:CDS | 50.0% | |
| GTTGGTTGGAGTGGAAGGAA+AGG | + | chr5_2:72862987-72863006 | Msa0787220:CDS | 50.0% | |
| ! | GCGGTGACAACAGATCCTTT+TGG | - | chr5_2:72863066-72863085 | None:intergenic | 50.0% |
| AAACCCTAACCCTGTCACAG+CGG | - | chr5_2:72863095-72863114 | None:intergenic | 50.0% | |
| CTGTGACAGGGTTAGGGTTT+GGG | + | chr5_2:72863095-72863114 | Msa0787220:CDS | 50.0% | |
| ! | AGCTCATGGCCAGAGTTGGT+TGG | + | chr5_2:72862973-72862992 | Msa0787220:CDS | 55.0% |
| ! | ATGGCCAGAGTTGGTTGGAG+TGG | + | chr5_2:72862978-72862997 | Msa0787220:CDS | 55.0% |
| CCTTCCACTCCAACCAACTC+TGG | - | chr5_2:72862985-72863004 | None:intergenic | 55.0% | |
| ! | CCAGAGTTGGTTGGAGTGGA+AGG | + | chr5_2:72862982-72863001 | Msa0787220:CDS | 55.0% |
| AACCCTGTCACAGCGGAAGT+CGG | - | chr5_2:72863088-72863107 | None:intergenic | 55.0% | |
| ACTTCCGCTGTGACAGGGTT+AGG | + | chr5_2:72863088-72863107 | Msa0787220:CDS | 55.0% | |
| CTTCCGCTGTGACAGGGTTA+GGG | + | chr5_2:72863089-72863108 | Msa0787220:CDS | 55.0% | |
| GCTGTGACAGGGTTAGGGTT+TGG | + | chr5_2:72863094-72863113 | Msa0787220:CDS | 55.0% | |
| CCTGTCACAGCGGAAGTCGG+CGG | - | chr5_2:72863085-72863104 | None:intergenic | 65.0% | |
| !! | CGCCGACTTCCGCTGTGACA+GGG | + | chr5_2:72863083-72863102 | Msa0787220:CDS | 65.0% |
| !! | CCGCCGACTTCCGCTGTGAC+AGG | + | chr5_2:72863082-72863101 | Msa0787220:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 72862753 | 72863389 | 72862753 | ID=Msa0787220;Name=Msa0787220 |
| chr5_2 | mRNA | 72862753 | 72863389 | 72862753 | ID=Msa0787220-mRNA-1;Parent=Msa0787220;Name=Msa0787220-mRNA-1;_AED=0.02;_eAED=0.02;_QI=100|1|1|1|1|1|2|228|70 |
| chr5_2 | exon | 72862753 | 72862871 | 72862753 | ID=Msa0787220-mRNA-1:exon:22135;Parent=Msa0787220-mRNA-1 |
| chr5_2 | exon | 72862968 | 72863389 | 72862968 | ID=Msa0787220-mRNA-1:exon:22136;Parent=Msa0787220-mRNA-1 |
| chr5_2 | five_prime_UTR | 72862753 | 72862852 | 72862753 | ID=Msa0787220-mRNA-1:five_prime_utr;Parent=Msa0787220-mRNA-1 |
| chr5_2 | CDS | 72862853 | 72862871 | 72862853 | ID=Msa0787220-mRNA-1:cds;Parent=Msa0787220-mRNA-1 |
| chr5_2 | CDS | 72862968 | 72863161 | 72862968 | ID=Msa0787220-mRNA-1:cds;Parent=Msa0787220-mRNA-1 |
| chr5_2 | three_prime_UTR | 72863162 | 72863389 | 72863162 | ID=Msa0787220-mRNA-1:three_prime_utr;Parent=Msa0787220-mRNA-1 |
| Gene Sequence |
| Protein sequence |
