AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0077370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077370 MtrunA17_Chr1g0187161 84.286 70 11 0 1 70 1 70 4.87e-37 118
Msa0077370 MtrunA17_Chr1g0187201 88.235 68 8 0 3 70 4 71 8.29e-37 117
Msa0077370 MtrunA17_Chr1g0187221 81.429 70 13 0 1 70 3 72 1.67e-35 114
Msa0077370 MtrunA17_Chr5g0442291 82.857 70 12 0 1 70 1 70 3.98e-35 113
Msa0077370 MtrunA17_Chr1g0184411 54.286 70 32 0 1 70 1 70 1.24e-20 77.0
Msa0077370 MtrunA17_Chr1g0184401 53.030 66 31 0 5 70 8 73 6.82e-18 70.1
Msa0077370 MtrunA17_Chr5g0419531 53.030 66 31 0 5 70 4 69 1.72e-17 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077370 AT2G38870.1 52.857 70 33 0 1 70 1 70 1.55e-20 77.0
Msa0077370 AT5G43580.1 48.611 72 35 1 1 70 28 99 8.35e-18 70.9
Msa0077370 AT3G46860.1 42.857 70 37 1 4 70 16 85 6.68e-13 58.2
Msa0077370 AT2G38900.2 45.312 64 35 0 7 70 25 88 1.56e-12 57.4
Msa0077370 AT2G38900.1 45.312 64 35 0 7 70 10 73 1.94e-12 56.6
Msa0077370 AT5G43570.1 46.575 73 33 1 1 67 1 73 1.43e-11 55.1

Find 33 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCAATTCTTCTTGTGTT+TGG 0.271522 1_2:-54247474 None:intergenic
CAACGACAGGGTTTGGGTTT+GGG 0.289389 1_2:+54247808 Msa0077370:CDS
GCAACGACAGGGTTTGGGTT+TGG 0.327218 1_2:+54247807 Msa0077370:CDS
GACACTTTCTATCCAATTGT+TGG 0.347121 1_2:-54247860 None:intergenic
ATAAATTTAATGTTTCAATA+AGG 0.348781 1_2:+54247915 Msa0077370:three_prime_UTR
ACTTACGCAACGACAGGGTT+TGG 0.351229 1_2:+54247801 Msa0077370:CDS
CTTACGCAACGACAGGGTTT+GGG 0.360903 1_2:+54247802 Msa0077370:CDS
GTCACTCAGGTTCCAACAAT+TGG 0.363160 1_2:+54247848 Msa0077370:CDS
TATAATAAGTAGTATACAAT+TGG 0.368616 1_2:-54247951 None:intergenic
TGGGTTTGGGTTAATAAAGA+TGG 0.418377 1_2:+54247821 Msa0077370:CDS
TACATATTGAAGGATCAAAA+AGG 0.425186 1_2:+54247526 Msa0077370:five_prime_UTR
CCTTCCACTCCAACCAACTC+TGG 0.454716 1_2:-54247695 None:intergenic
AATGCCATCATTGTGCTAGA+AGG 0.471882 1_2:+54247761 Msa0077370:CDS
GAAACATTAAATTTATGACT+TGG 0.485653 1_2:-54247908 None:intergenic
AAAATAGTCATACATATTGA+AGG 0.494025 1_2:+54247516 Msa0077370:five_prime_UTR
CCAGAGTTGGTTGGAGTGGA+AGG 0.515293 1_2:+54247695 Msa0077370:CDS
TGTTCTCAGCTAAGAGCTCA+TGG 0.516002 1_2:+54247672 Msa0077370:intron
CCGACGACTTACGCAACGAC+AGG 0.517888 1_2:+54247795 Msa0077370:CDS
GTTGGTTGGAGTGGAAGGAA+AGG 0.527079 1_2:+54247700 Msa0077370:CDS
CAGTGTCATATATAATTCCT+TGG 0.530851 1_2:-54248045 None:intergenic
AGATCCTTCTAGCACAATGA+TGG 0.537596 1_2:-54247765 None:intergenic
ATGGCCAGAGTTGGTTGGAG+TGG 0.564909 1_2:+54247691 Msa0077370:CDS
AATTCAGAGGGAGAATCCAT+TGG 0.572843 1_2:+54247736 Msa0077370:CDS
TAAGAGCTCATGGCCAGAGT+TGG 0.573671 1_2:+54247682 Msa0077370:CDS
AGCTCATGGCCAGAGTTGGT+TGG 0.578782 1_2:+54247686 Msa0077370:CDS
TAAAGATGGAATTGTCACTC+AGG 0.589757 1_2:+54247835 Msa0077370:CDS
AGCAGAAGCTACAATTCAGA+GGG 0.593359 1_2:+54247724 Msa0077370:CDS
TAGCAGAAGCTACAATTCAG+AGG 0.596029 1_2:+54247723 Msa0077370:CDS
CCTGTCGTTGCGTAAGTCGT+CGG 0.616539 1_2:-54247795 None:intergenic
CGACGACTTACGCAACGACA+GGG 0.619969 1_2:+54247796 Msa0077370:CDS
CAATTGGATAGAAAGTGTCA+TGG 0.634335 1_2:+54247864 Msa0077370:exon
ACAATGATGGCATTCACCAA+TGG 0.650325 1_2:-54247752 None:intergenic
AAAATGTCTGATGAATGCAA+AGG 0.675933 1_2:+54247555 Msa0077370:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATGAATAATAAATTAAA+TGG + chr1_2:54247602-54247621 Msa0077370:intron 10.0%
!! ATAAATTTAATGTTTCAATA+AGG + chr1_2:54247915-54247934 Msa0077370:three_prime_UTR 10.0%
!! TATAATAAGTAGTATACAAT+TGG - chr1_2:54247954-54247973 None:intergenic 15.0%
!! AATTGCAAAAAGAAAGAAAA+AGG + chr1_2:54247488-54247507 Msa0077370:five_prime_UTR 20.0%
!! AAAATAGTCATACATATTGA+AGG + chr1_2:54247516-54247535 Msa0077370:five_prime_UTR 20.0%
!! GAAACATTAAATTTATGACT+TGG - chr1_2:54247911-54247930 None:intergenic 20.0%
! TACATATTGAAGGATCAAAA+AGG + chr1_2:54247526-54247545 Msa0077370:five_prime_UTR 25.0%
!!! GTTTTTTATTTTCTATGCCA+AGG + chr1_2:54248028-54248047 Msa0077370:three_prime_UTR 25.0%
TTTGCAATTCTTCTTGTGTT+TGG - chr1_2:54247477-54247496 None:intergenic 30.0%
AAAATGTCTGATGAATGCAA+AGG + chr1_2:54247555-54247574 Msa0077370:exon 30.0%
CAGTGTCATATATAATTCCT+TGG - chr1_2:54248048-54248067 None:intergenic 30.0%
! TGGGTTTGGGTTAATAAAGA+TGG + chr1_2:54247821-54247840 Msa0077370:CDS 35.0%
TAAAGATGGAATTGTCACTC+AGG + chr1_2:54247835-54247854 Msa0077370:CDS 35.0%
GACACTTTCTATCCAATTGT+TGG - chr1_2:54247863-54247882 None:intergenic 35.0%
!! CAATTGGATAGAAAGTGTCA+TGG + chr1_2:54247864-54247883 Msa0077370:exon 35.0%
GTCTGATGAATGCAAAGGTA+AGG + chr1_2:54247560-54247579 Msa0077370:intron 40.0%
TAGCAGAAGCTACAATTCAG+AGG + chr1_2:54247723-54247742 Msa0077370:CDS 40.0%
AGCAGAAGCTACAATTCAGA+GGG + chr1_2:54247724-54247743 Msa0077370:CDS 40.0%
AATTCAGAGGGAGAATCCAT+TGG + chr1_2:54247736-54247755 Msa0077370:CDS 40.0%
ACAATGATGGCATTCACCAA+TGG - chr1_2:54247755-54247774 None:intergenic 40.0%
AATGCCATCATTGTGCTAGA+AGG + chr1_2:54247761-54247780 Msa0077370:CDS 40.0%
AGATCCTTCTAGCACAATGA+TGG - chr1_2:54247768-54247787 None:intergenic 40.0%
TGTTCTCAGCTAAGAGCTCA+TGG + chr1_2:54247672-54247691 Msa0077370:intron 45.0%
GTCACTCAGGTTCCAACAAT+TGG + chr1_2:54247848-54247867 Msa0077370:CDS 45.0%
!!! AAATTTTTATTATAATTTGA+TGG + chr1_2:54247631-54247650 Msa0077370:intron 5.0%
TAAGAGCTCATGGCCAGAGT+TGG + chr1_2:54247682-54247701 Msa0077370:CDS 50.0%
GTTGGTTGGAGTGGAAGGAA+AGG + chr1_2:54247700-54247719 Msa0077370:CDS 50.0%
ACTTACGCAACGACAGGGTT+TGG + chr1_2:54247801-54247820 Msa0077370:CDS 50.0%
CTTACGCAACGACAGGGTTT+GGG + chr1_2:54247802-54247821 Msa0077370:CDS 50.0%
! CAACGACAGGGTTTGGGTTT+GGG + chr1_2:54247808-54247827 Msa0077370:CDS 50.0%
! AGCTCATGGCCAGAGTTGGT+TGG + chr1_2:54247686-54247705 Msa0077370:CDS 55.0%
! ATGGCCAGAGTTGGTTGGAG+TGG + chr1_2:54247691-54247710 Msa0077370:CDS 55.0%
CCTTCCACTCCAACCAACTC+TGG - chr1_2:54247698-54247717 None:intergenic 55.0%
! CCAGAGTTGGTTGGAGTGGA+AGG + chr1_2:54247695-54247714 Msa0077370:CDS 55.0%
CCTGTCGTTGCGTAAGTCGT+CGG - chr1_2:54247798-54247817 None:intergenic 55.0%
CGACGACTTACGCAACGACA+GGG + chr1_2:54247796-54247815 Msa0077370:CDS 55.0%
! GCAACGACAGGGTTTGGGTT+TGG + chr1_2:54247807-54247826 Msa0077370:CDS 55.0%
CCGACGACTTACGCAACGAC+AGG + chr1_2:54247795-54247814 Msa0077370:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 54247457 54248081 54247457 ID=Msa0077370;Name=Msa0077370
chr1_2 mRNA 54247457 54248081 54247457 ID=Msa0077370-mRNA-1;Parent=Msa0077370;Name=Msa0077370-mRNA-1;_AED=0.25;_eAED=0.25;_QI=101|1|1|1|1|1|2|207|70
chr1_2 exon 54247457 54247576 54247457 ID=Msa0077370-mRNA-1:exon:16735;Parent=Msa0077370-mRNA-1
chr1_2 exon 54247681 54248081 54247681 ID=Msa0077370-mRNA-1:exon:16736;Parent=Msa0077370-mRNA-1
chr1_2 five_prime_UTR 54247457 54247557 54247457 ID=Msa0077370-mRNA-1:five_prime_utr;Parent=Msa0077370-mRNA-1
chr1_2 CDS 54247558 54247576 54247558 ID=Msa0077370-mRNA-1:cds;Parent=Msa0077370-mRNA-1
chr1_2 CDS 54247681 54247874 54247681 ID=Msa0077370-mRNA-1:cds;Parent=Msa0077370-mRNA-1
chr1_2 three_prime_UTR 54247875 54248081 54247875 ID=Msa0077370-mRNA-1:three_prime_utr;Parent=Msa0077370-mRNA-1
Gene Sequence

>Msa0077370

ATGTCTGATGAATGCAAAGCTAAGAGCTCATGGCCAGAGTTGGTTGGAGTGGAAGGAAAGGTAGCAGAAGCTACAATTCAGAGGGAGAATCCATTGGTGAATGCCATCATTGTGCTAGAAGGATCTTCTGTCACCGACGACTTACGCAACGACAGGGTTTGGGTTTGGGTTAATAAAGATGGAATTGTCACTCAGGTTCCAACAATTGGATAG

Protein sequence

>Msa0077370

MSDECKAKSSWPELVGVEGKVAEATIQRENPLVNAIIVLEGSSVTDDLRNDRVWVWVNKDGIVTQVPTIG*