AlfalfaGEDB | Msa0797280

M. sativa cultivar zhongmu-4 / Msa0797280

KEGG: -
GO: GO:0000325 GO:0003674 GO:0005215 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0008150 GO:0008509 GO:0008514 GO:0009705 GO:0009987 GO:0010118 GO:0012505 GO:0015075 GO:0015140 GO:0015318 GO:0015556 GO:0015711 GO:0015740 GO:0015743 GO:0015849 GO:0016020 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071423 GO:0071702 GO:0098588 GO:0098656 GO:0098805 GO:1903825 GO:1905039
PFAM: PF11744.11

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0797280	XP_003611471.1	98.198	111	2	0	1	111	109	219	7.10e-67	220

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0797280	sp\|O49696\|ALMTC_ARATH	69.369	111	33	1	1	111	91	200	3.48e-49	167

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0797280	G7JZX5	98.198	111	2	0	1	111	109	219	3.39e-67	220

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0797270	Msa0797280	0.818995	1.464968e-52	-8.615850e-47
Msa0797280	Msa0978000	0.826916	2.086380e-54	-8.615850e-47
Msa0797280	Msa1044040	0.801616	8.278079e-49	-8.615850e-47
Msa0797280	Msa1274310	0.805114	1.560471e-49	-8.615850e-47
Msa0797280	Msa1340880	0.800059	1.721091e-48	-8.615850e-47
Msa0797280	Msa1366620	0.801929	7.141543e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0797280	MtrunA17_Chr5g0400471	100.000	107	0	0	5	111	3	109	6.63e-70	216
Msa0797280	MtrunA17_Chr1g0212291	67.568	111	35	1	1	111	90	199	7.01e-48	160
Msa0797280	MtrunA17_Chr1g0212201	64.865	111	38	1	1	111	93	202	5.75e-45	152
Msa0797280	MtrunA17_Chr1g0212251	64.865	111	38	1	1	111	89	198	7.53e-45	152
Msa0797280	MtrunA17_Chr4g0054741	74.775	111	27	1	1	111	101	210	5.60e-42	144
Msa0797280	MtrunA17_Chr1g0188881	46.789	109	57	1	3	111	106	213	3.42e-30	111
Msa0797280	MtrunA17_Chr7g0267471	48.148	108	55	1	4	111	97	203	7.41e-30	110
Msa0797280	MtrunA17_Chr1g0188871	47.706	109	56	1	3	111	100	207	8.16e-30	110
Msa0797280	MtrunA17_Chr4g0025591	47.222	108	56	1	4	111	96	202	2.90e-28	106
Msa0797280	MtrunA17_Chr4g0054771	70.000	60	18	0	52	111	3	62	7.07e-26	99.0
Msa0797280	MtrunA17_Chr2g0321971	43.243	111	61	1	1	109	89	199	4.83e-25	97.4
Msa0797280	MtrunA17_Chr7g0259731	43.750	112	59	3	3	111	110	220	1.34e-23	93.6
Msa0797280	MtrunA17_Chr6g0484691	39.450	109	65	1	1	109	136	243	8.71e-20	83.2
Msa0797280	MtrunA17_Chr2g0321961	40.541	111	65	1	1	111	89	198	2.86e-19	81.3
Msa0797280	MtrunA17_Chr8g0390901	39.450	109	63	2	3	111	9	114	8.82e-17	74.3
Msa0797280	MtrunA17_Chr8g0390921	36.937	111	67	2	1	111	111	218	4.20e-15	69.7
Msa0797280	MtrunA17_Chr8g0390931	38.739	111	65	2	1	111	112	219	3.26e-13	64.3
Msa0797280	MtrunA17_Chr1g0210101	32.432	111	73	2	1	111	163	271	4.93e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0797280	AT4G17970.1	69.369	111	33	1	1	111	91	200	3.54e-50	167
Msa0797280	AT5G46610.1	70.270	111	32	1	1	111	93	202	1.00e-49	165
Msa0797280	AT5G46600.1	63.063	111	40	1	1	111	94	203	9.32e-44	149
Msa0797280	AT5G46610.2	65.556	90	30	1	22	111	90	178	2.29e-35	126
Msa0797280	AT4G00910.1	44.444	108	58	1	4	111	113	218	1.58e-28	107
Msa0797280	AT3G11680.2	46.296	108	57	1	4	111	93	199	4.78e-28	106
Msa0797280	AT5G46610.3	73.016	63	17	0	49	111	93	155	1.10e-27	105
Msa0797280	AT3G11680.1	46.296	108	57	1	4	111	91	197	1.46e-26	102
Msa0797280	AT1G08440.1	40.541	111	65	1	1	111	65	174	9.58e-26	100
Msa0797280	AT1G08430.1	39.640	111	66	1	1	111	68	177	4.34e-24	95.5
Msa0797280	AT2G27240.2	41.441	111	64	1	1	111	65	174	1.68e-23	93.6
Msa0797280	AT2G27240.1	41.441	111	64	1	1	111	65	174	2.34e-23	93.6
Msa0797280	AT3G18440.1	37.963	108	64	2	4	111	134	238	1.49e-15	71.2
Msa0797280	AT1G18420.1	35.135	111	69	2	1	111	135	242	4.73e-15	69.7
Msa0797280	AT1G18420.2	35.135	111	69	2	1	111	52	159	7.18e-15	69.3
Msa0797280	AT2G17470.2	34.545	110	69	2	2	111	106	212	2.82e-13	64.7
Msa0797280	AT2G17470.1	34.545	110	69	2	2	111	84	190	2.89e-13	64.7
Msa0797280	AT1G68600.1	37.037	108	65	2	4	111	103	207	4.02e-13	64.3

Find 21 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACACTGTTAGCTGGATTAT+TGG	0.245806	5_3:-9351092	Msa0797280:CDS
TCATAGTTCTTCTTTATATA+AGG	0.253399	5_3:+9350596	None:intergenic
GGATTATTGGCATTCATTGT+TGG	0.386550	5_3:-9351079	Msa0797280:CDS
AAAGGGCTTAACAGAGGATT+GGG	0.400724	5_3:-9351115	Msa0797280:CDS
ATAAAGAAGAACTATGACTA+TGG	0.401454	5_3:-9350590	Msa0797280:CDS
GAGCTTTAGCTACTTATATG+AGG	0.435607	5_3:-9350625	Msa0797280:CDS
CAAAGGGCTTAACAGAGGAT+TGG	0.468379	5_3:-9351116	Msa0797280:CDS
AAGGCAAATGGCACATCCTA+TGG	0.471313	5_3:+9350469	None:intergenic
TATGCTCATTATAAGGCAAA+TGG	0.472401	5_3:+9350457	None:intergenic
ATCTCTCAAGCTGTTATCAT+TGG	0.475130	5_3:-9351028	Msa0797280:CDS
CAGGGGCAACTTTATGCAAA+GGG	0.490713	5_3:-9351132	Msa0797280:CDS
GGATTGGGGACACTGTTAGC+TGG	0.502209	5_3:-9351100	Msa0797280:CDS
GGCACATCCTATGGCAATGG+AGG	0.551636	5_3:+9350478	None:intergenic
GCAGGGGCAACTTTATGCAA+AGG	0.555764	5_3:-9351133	Msa0797280:CDS
CGCATATCCTCCATTGCCAT+AGG	0.573769	5_3:-9350485	Msa0797280:CDS
TTGCAAATGCATCATCTCAT+CGG	0.574979	5_3:-9351051	Msa0797280:CDS
AATGGCACATCCTATGGCAA+TGG	0.578492	5_3:+9350475	None:intergenic
TTATGCAAAGGGCTTAACAG+AGG	0.619114	5_3:-9351121	Msa0797280:CDS
TCTTCAACACACTGTCCACA+CGG	0.631610	5_3:+9350519	None:intergenic
TGCTTTGTCAAGTTACCGTG+TGG	0.668331	5_3:-9350534	Msa0797280:CDS
AAGGGCTTAACAGAGGATTG+GGG	0.729918	5_3:-9351114	Msa0797280:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCATAGTTCTTCTTTATATA+AGG	+	chr5_3:9351015-9351034	None:intergenic	20.0%
!	AATTCTGTTACATTAATCAC+TGG	+	chr5_3:9350789-9350808	None:intergenic	25.0%
!	AATGTTAACAAATAGTCACA+AGG	-	chr5_3:9350871-9350890	Msa0797280:intron	25.0%
!	ATAAAGAAGAACTATGACTA+TGG	-	chr5_3:9351018-9351037	Msa0797280:CDS	25.0%
	TATATGAGATCTCGATCATA+TGG	-	chr5_3:9350657-9350676	Msa0797280:intron	30.0%
!	TCTTAATATGCGATTTTCTG+CGG	-	chr5_3:9350946-9350965	Msa0797280:intron	30.0%
	GGATTATTGGCATTCATTGT+TGG	-	chr5_3:9350529-9350548	Msa0797280:CDS	35.0%
	TTGCAAATGCATCATCTCAT+CGG	-	chr5_3:9350557-9350576	Msa0797280:CDS	35.0%
	ATCTCTCAAGCTGTTATCAT+TGG	-	chr5_3:9350580-9350599	Msa0797280:CDS	35.0%
	ATGATCGAGATCTCATATAG+CGG	+	chr5_3:9350656-9350675	None:intergenic	35.0%
	GGTTTGAATACCTCGTATTT+CGG	-	chr5_3:9350678-9350697	Msa0797280:intron	35.0%
	ATGGTTTGAACAAGTCGAAT+TGG	-	chr5_3:9350710-9350729	Msa0797280:intron	35.0%
!	GAGCTTTAGCTACTTATATG+AGG	-	chr5_3:9350983-9351002	Msa0797280:intron	35.0%
	TTATGCAAAGGGCTTAACAG+AGG	-	chr5_3:9350487-9350506	Msa0797280:CDS	40.0%
	AAAGGGCTTAACAGAGGATT+GGG	-	chr5_3:9350493-9350512	Msa0797280:CDS	40.0%
	GACACTGTTAGCTGGATTAT+TGG	-	chr5_3:9350516-9350535	Msa0797280:CDS	40.0%
	TGATCGAGATCTCATATAGC+GGG	+	chr5_3:9350655-9350674	None:intergenic	40.0%
	TTTGATGAAGCCGAAATACG+AGG	+	chr5_3:9350691-9350710	None:intergenic	40.0%
	CGTATTTCGGCTTCATCAAA+TGG	-	chr5_3:9350691-9350710	Msa0797280:intron	40.0%
!!	TTCTGCGGATTTGTTATTGC+AGG	-	chr5_3:9350961-9350980	Msa0797280:intron	40.0%
	CAGGGGCAACTTTATGCAAA+GGG	-	chr5_3:9350476-9350495	Msa0797280:CDS	45.0%
	CAAAGGGCTTAACAGAGGAT+TGG	-	chr5_3:9350492-9350511	Msa0797280:CDS	45.0%
	AAGGGCTTAACAGAGGATTG+GGG	-	chr5_3:9350494-9350513	Msa0797280:CDS	45.0%
!	GGCGCTGCAGTTTTTCTTAT+AGG	-	chr5_3:9350601-9350620	Msa0797280:CDS	45.0%
	GATCGAGATCTCATATAGCG+GGG	+	chr5_3:9350654-9350673	None:intergenic	45.0%
	TGCTTTGTCAAGTTACCGTG+TGG	-	chr5_3:9351074-9351093	Msa0797280:CDS	45.0%
	TCTTCAACACACTGTCCACA+CGG	+	chr5_3:9351092-9351111	None:intergenic	45.0%
	AATGGCACATCCTATGGCAA+TGG	+	chr5_3:9351136-9351155	None:intergenic	45.0%
	AAGGCAAATGGCACATCCTA+TGG	+	chr5_3:9351142-9351161	None:intergenic	45.0%
	GCAGGGGCAACTTTATGCAA+AGG	-	chr5_3:9350475-9350494	Msa0797280:CDS	50.0%
!	TCTCATATAGCGGGGAATCG+AGG	+	chr5_3:9350646-9350665	None:intergenic	50.0%
	CGCATATCCTCCATTGCCAT+AGG	-	chr5_3:9351123-9351142	Msa0797280:CDS	50.0%
	GGATTGGGGACACTGTTAGC+TGG	-	chr5_3:9350508-9350527	Msa0797280:CDS	55.0%
	GGCACATCCTATGGCAATGG+AGG	+	chr5_3:9351133-9351152	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	9350469	9351161	9350469	ID=Msa0797280;Name=Msa0797280
chr5_3	mRNA	9350469	9351161	9350469	ID=Msa0797280-mRNA-1;Parent=Msa0797280;Name=Msa0797280-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|1\|0\|1\|0\|0\|2\|0\|111
chr5_3	exon	9350469	9350647	9350469	ID=Msa0797280-mRNA-1:exon:4079;Parent=Msa0797280-mRNA-1
chr5_3	exon	9351008	9351161	9351008	ID=Msa0797280-mRNA-1:exon:4078;Parent=Msa0797280-mRNA-1
chr5_3	CDS	9351008	9351161	9351008	ID=Msa0797280-mRNA-1:cds;Parent=Msa0797280-mRNA-1
chr5_3	CDS	9350469	9350647	9350469	ID=Msa0797280-mRNA-1:cds;Parent=Msa0797280-mRNA-1

Gene Sequence	>Msa0797280 TTGGCTGCAGGGGCAACTTTATGCAAAGGGCTTAACAGAGGATTGGGGACACTGTTAGCTGGATTATTGGCATTCATTGTTGGCTATATTGCAAATGCATCATCTCATCGGATCTCTCAAGCTGTTATCATTGGCGCTGCAGTTTTTCTTATAGGAGCTTTAGCTACTTATATGAGGTTCATTCCTTATATAAAGAAGAACTATGACTATGGTCTAGTTATATTTCTCATGACTTTTAACTTGATTGCTTTGTCAAGTTACCGTGTGGACAGTGTGTTGAAGATAGCACATGAACGCATATCCTCCATTGCCATAGGATGTGCCATTTGCCTT
Protein sequence	>Msa0797280 LAAGATLCKGLNRGLGTLLAGLLAFIVGYIANASSHRISQAVIIGAAVFLIGALATYMRFIPYIKKNYDYGLVIFLMTFNLIALSSYRVDSVLKIAHERISSIAIGCAICL