AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0800710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800710 MtrunA17_Chr5g0407171 96.529 461 16 0 1 461 1 461 0.0 916
Msa0800710 MtrunA17_Chr8g0370261 69.048 462 142 1 1 461 1 462 0.0 673
Msa0800710 MtrunA17_Chr3g0144141 50.217 460 215 5 4 461 53 500 3.42e-155 465
Msa0800710 MtrunA17_Chr2g0322691 46.970 462 238 3 2 461 6 462 4.29e-141 427
Msa0800710 MtrunA17_Chr8g0370281 54.642 377 156 7 94 461 1 371 3.75e-133 405
Msa0800710 MtrunA17_Chr2g0322681 40.173 463 261 5 1 461 10 458 1.12e-117 366
Msa0800710 MtrunA17_Chr3g0136611 28.821 458 293 15 31 472 35 475 2.66e-46 172
Msa0800710 MtrunA17_Chr3g0136621 28.049 492 308 16 1 472 13 478 1.20e-45 170
Msa0800710 MtrunA17_Chr4g0046101 25.420 476 290 18 5 461 19 448 3.78e-28 118
Msa0800710 MtrunA17_Chr2g0283231 22.967 492 295 20 1 461 11 449 1.76e-24 107
Msa0800710 MtrunA17_Chr8g0370291 40.179 112 66 1 1 112 1 111 9.87e-22 90.9
Msa0800710 MtrunA17_Chr5g0424621 24.561 456 282 18 28 461 36 451 2.16e-15 78.6
Msa0800710 MtrunA17_Chr5g0424541 23.747 459 282 18 28 461 36 451 6.04e-13 71.2
Msa0800710 MtrunA17_Chr7g0230081 26.182 275 178 11 28 287 48 312 6.49e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800710 AT3G51480.3 51.923 468 213 3 5 472 3 458 4.31e-175 511
Msa0800710 AT3G51480.4 51.923 468 213 3 5 472 3 458 7.18e-174 512
Msa0800710 AT3G51480.1 51.923 468 213 3 5 472 3 458 7.18e-174 512
Msa0800710 AT1G42540.3 51.810 442 203 5 23 461 21 455 3.71e-159 466
Msa0800710 AT1G42540.1 51.810 442 203 5 23 461 21 455 5.88e-156 466
Msa0800710 AT1G42540.4 51.810 442 203 5 23 461 21 455 5.88e-156 466
Msa0800710 AT1G42540.2 51.810 442 203 5 23 461 21 455 5.88e-156 466
Msa0800710 AT4G35290.6 47.722 461 231 5 5 461 3 457 1.07e-145 435
Msa0800710 AT3G51480.2 53.826 379 164 2 94 472 1 368 3.03e-144 433
Msa0800710 AT4G35290.4 47.722 461 231 5 5 461 3 457 3.42e-144 436
Msa0800710 AT4G35290.3 47.722 461 231 5 5 461 3 457 3.42e-144 436
Msa0800710 AT4G35290.1 47.722 461 231 5 5 461 3 457 3.42e-144 436
Msa0800710 AT4G35290.2 47.722 461 231 5 5 461 3 457 3.42e-144 436
Msa0800710 AT4G35290.5 47.722 461 231 5 5 461 3 457 3.42e-144 436
Msa0800710 AT1G05200.4 49.078 434 214 2 28 461 57 483 8.04e-144 427
Msa0800710 AT1G05200.5 49.078 434 214 2 28 461 57 483 9.22e-144 427
Msa0800710 AT2G32390.7 47.674 430 224 1 32 461 48 476 2.96e-142 422
Msa0800710 AT2G32390.6 47.674 430 224 1 32 461 48 476 5.50e-142 422
Msa0800710 AT1G05200.7 49.078 434 214 2 28 461 57 483 5.53e-142 427
Msa0800710 AT1G05200.3 49.078 434 214 2 28 461 57 483 5.53e-142 427
Msa0800710 AT2G32390.4 47.674 430 224 1 32 461 48 476 1.05e-141 422
Msa0800710 AT2G32390.3 47.674 430 224 1 32 461 48 476 1.05e-141 422
Msa0800710 AT1G05200.6 49.078 434 214 2 28 461 57 483 9.70e-141 428
Msa0800710 AT1G05200.2 49.078 434 214 2 28 461 57 483 9.70e-141 428
Msa0800710 AT1G05200.1 49.078 434 214 2 28 461 57 483 9.70e-141 428
Msa0800710 AT2G17260.1 47.609 460 231 5 5 461 7 459 8.33e-140 424
Msa0800710 AT2G32390.5 47.674 430 224 1 32 461 48 476 2.85e-139 424
Msa0800710 AT2G32390.2 48.787 371 190 0 91 461 4 374 1.65e-123 380
Msa0800710 AT2G32390.1 48.378 370 191 0 92 461 49 418 9.93e-122 377
Msa0800710 AT2G32400.1 40.961 437 246 3 25 461 24 448 1.58e-105 335
Msa0800710 AT2G29100.2 32.333 433 264 12 32 461 32 438 5.71e-59 209
Msa0800710 AT2G29100.1 32.333 433 264 12 32 461 30 436 5.87e-59 209
Msa0800710 AT5G27100.1 30.134 448 281 13 32 472 32 454 2.74e-51 187
Msa0800710 AT2G29110.1 29.698 431 283 8 32 461 33 444 1.28e-50 185
Msa0800710 AT2G29110.2 29.698 431 283 8 32 461 34 445 1.68e-50 185
Msa0800710 AT2G24720.1 29.372 446 287 10 32 472 32 454 4.85e-50 183
Msa0800710 AT5G11210.3 31.283 374 235 8 90 461 20 373 5.09e-50 182
Msa0800710 AT4G31710.1 29.103 457 295 13 21 472 21 453 1.43e-49 182
Msa0800710 AT4G31710.2 29.103 457 295 13 21 472 44 476 2.25e-49 181
Msa0800710 AT5G11210.7 30.997 371 234 8 93 461 15 365 2.51e-49 180
Msa0800710 AT5G11210.6 30.997 371 234 8 93 461 15 365 2.51e-49 180
Msa0800710 AT5G11210.8 30.997 371 234 8 93 461 15 365 2.70e-49 179
Msa0800710 AT2G24710.2 29.070 430 282 10 46 472 69 478 2.83e-49 181
Msa0800710 AT5G11210.2 30.997 371 234 8 93 461 97 447 3.99e-49 180
Msa0800710 AT5G11210.4 30.997 371 234 8 93 461 97 447 3.99e-49 180
Msa0800710 AT2G24710.1 28.837 430 283 10 46 472 45 454 4.23e-49 181
Msa0800710 AT5G11210.10 30.997 371 234 8 93 461 97 447 4.35e-49 180
Msa0800710 AT2G24710.3 28.837 430 283 10 46 472 69 478 5.42e-49 180
Msa0800710 AT5G11210.9 30.997 371 234 8 93 461 15 365 6.52e-49 179
Msa0800710 AT5G11210.1 30.997 371 234 8 93 461 39 389 7.22e-49 180
Msa0800710 AT5G11210.5 30.997 371 234 8 93 461 97 447 7.70e-49 180
Msa0800710 AT2G29120.1 28.146 437 283 12 32 461 39 451 3.38e-48 178
Msa0800710 AT5G11180.2 28.140 430 295 6 32 461 36 451 8.46e-48 177
Msa0800710 AT5G11180.1 28.140 430 295 6 32 461 36 451 1.11e-47 176
Msa0800710 AT3G07520.1 23.340 467 303 11 5 461 12 433 3.06e-30 125
Msa0800710 AT5G48400.1 24.374 439 275 13 32 461 40 430 5.23e-29 121
Msa0800710 AT5G48400.2 24.374 439 275 13 32 461 40 430 8.90e-29 120
Msa0800710 AT5G48410.2 23.570 437 277 11 32 461 42 428 2.59e-27 116
Msa0800710 AT5G48410.4 23.744 438 275 12 32 461 42 428 2.64e-27 116
Msa0800710 AT5G48410.3 23.744 438 275 12 32 461 42 428 3.04e-27 115
Msa0800710 AT5G48410.1 23.744 438 275 12 32 461 42 428 3.55e-27 115
Msa0800710 AT3G04110.1 23.362 458 298 11 6 461 4 410 6.40e-24 105
Msa0800710 AT3G04110.2 23.362 458 298 11 6 461 4 410 7.07e-24 105
Msa0800710 AT3G04110.3 23.362 458 298 11 6 461 4 410 8.52e-24 105

Find 122 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCAGAAGTTGGATATTT+TGG 0.108501 5_3:+14209009 None:intergenic
TGAAAACATGTGAGAAATTT+TGG 0.147502 5_3:+14209105 None:intergenic
GGTTTGGAATTAAGTGTTTC+TGG 0.159851 5_3:+14206798 None:intergenic
TCGCGACACTAAACACTTTC+GGG 0.205568 5_3:+14207381 None:intergenic
TCTTTCTTTGTTCCTACTTT+TGG 0.219865 5_3:-14208846 Msa0800710:five_prime_UTR
TTTAAGCATTGTGGAGAAAA+TGG 0.225840 5_3:+14208870 None:intergenic
GGCTTCTGAGCTGTGTTTCC+AGG 0.230286 5_3:+14206729 None:intergenic
TTGGTCTCGCCCAGAATATT+TGG 0.240711 5_3:+14208204 None:intergenic
AAAGAAAGTTTGTTTCTAAA+TGG 0.241296 5_3:-14207205 Msa0800710:CDS
GTGTGAGTTTCATTATTTCT+TGG 0.280628 5_3:+14209221 None:intergenic
GCTCCAATATTTACAATATC+AGG 0.286846 5_3:+14208306 None:intergenic
AAATTCGGATCCAAATATTC+TGG 0.296117 5_3:-14208214 Msa0800710:CDS
CCTGAAGTTATTGCCTGGTT+TGG 0.296388 5_3:+14206782 None:intergenic
AGGCCTGATATTGTAAATAT+TGG 0.301238 5_3:-14208309 Msa0800710:CDS
ATCTCCTCTGCAGTTGCTTC+GGG 0.310294 5_3:+14207476 None:intergenic
GTTTCATTATTTCTTGGCTT+TGG 0.318226 5_3:+14209227 None:intergenic
AGACACAATGTGGCAATAAT+TGG 0.319777 5_3:-14207800 Msa0800710:CDS
AAGACATACCTTAGAGCTTC+AGG 0.319955 5_3:+14208769 None:intergenic
TTCGCGACACTAAACACTTT+CGG 0.325739 5_3:+14207380 None:intergenic
TGAGATTCTGCAACGCTTCT+CGG 0.330906 5_3:+14207517 None:intergenic
AAATGCTATTCCCTGTGATT+TGG 0.337239 5_3:-14206752 Msa0800710:CDS
TATCTCCTCTGCAGTTGCTT+CGG 0.342196 5_3:+14207475 None:intergenic
GTAGTTCACGCCAACACTAT+TGG 0.344238 5_3:-14207407 Msa0800710:CDS
ATCATCAATGTGGTTGGAAC+CGG 0.347020 5_3:-14206873 Msa0800710:CDS
ATACAACCATGGTTAAAGTT+TGG 0.351589 5_3:-14208398 Msa0800710:exon
TAGTTCACGCCAACACTATT+GGG 0.355308 5_3:-14207406 Msa0800710:CDS
GCAGACCTTGTTGACTACTA+TGG 0.377656 5_3:-14207617 Msa0800710:CDS
CATTCACAATTCTAAGCTAC+TGG 0.391368 5_3:-14208814 Msa0800710:five_prime_UTR
TTTAGTGTCGCGAAGAATCT+TGG 0.392243 5_3:-14207371 Msa0800710:CDS
CAAGAAGATTCTAAGTATAG+AGG 0.393980 5_3:-14208165 Msa0800710:CDS
GGTCTCGGAGGATCGGTTAT+TGG 0.400571 5_3:-14206851 Msa0800710:CDS
ATATCCTTCCTGAAGCTCTA+AGG 0.401191 5_3:-14208777 Msa0800710:intron
GATAACAAATGTGCTAGTTC+AGG 0.402634 5_3:-14207456 Msa0800710:CDS
CTCTCCCTGAAGTTATTGCC+TGG 0.405725 5_3:+14206777 None:intergenic
CCAAACCAGGCAATAACTTC+AGG 0.406252 5_3:-14206782 Msa0800710:CDS
CGCGAAGAATCTTGGAATGA+TGG 0.420925 5_3:-14207363 Msa0800710:CDS
AGGAACGGAATTGGTGCATT+AGG 0.427427 5_3:-14207551 Msa0800710:CDS
GGCTTCTCTTCAACTGTGGT+TGG 0.431482 5_3:-14208348 Msa0800710:CDS
TCACAGTGATCTTTATCAAA+TGG 0.431568 5_3:-14207648 Msa0800710:CDS
TGACAAGTTCTGATAAAGAA+TGG 0.432372 5_3:+14207668 None:intergenic
AATCATTTGAGAATGTGATT+TGG 0.434174 5_3:+14207060 None:intergenic
CTATTCCCTGTGATTTGGCC+TGG 0.435796 5_3:-14206747 Msa0800710:CDS
GTATACATTGATGATGATAA+TGG 0.436911 5_3:-14207575 Msa0800710:CDS
TGCGGTGAACGATGTAAATT+CGG 0.437111 5_3:-14208229 Msa0800710:CDS
CAACTTCTGAGGAGTGTGAT+TGG 0.440361 5_3:-14208999 Msa0800710:five_prime_UTR
CGCGTGCTGTCAACACTCCT+TGG 0.443293 5_3:+14207252 None:intergenic
ACGCGTGCACACGCCAGATT+CGG 0.452627 5_3:-14207234 Msa0800710:CDS
GTACGGACAAGAGGGACAAT+TGG 0.472644 5_3:+14209065 None:intergenic
ATGTGGTTGGAACCGGGTCT+CGG 0.476531 5_3:-14206866 Msa0800710:CDS
TTGAAGAGAGAGCTTTAACT+TGG 0.478917 5_3:+14208185 None:intergenic
ATTAAGTGTTTCTGGAGAAA+CGG 0.481795 5_3:+14206806 None:intergenic
CTTAGAATTGTGAATGAAGA+AGG 0.482617 5_3:+14208821 None:intergenic
CAATCACACTCCTCAGAAGT+TGG 0.482973 5_3:+14209000 None:intergenic
AATACTGATAGTGGCCCTCT+TGG 0.485612 5_3:-14207164 Msa0800710:CDS
GCAGTTGCTTCGGGTCTCAC+AGG 0.488039 5_3:+14207485 None:intergenic
GACAAGTTCTGATAAAGAAT+GGG 0.488714 5_3:+14207669 None:intergenic
ACATTGATGATTTCATATGT+AGG 0.491964 5_3:+14206885 None:intergenic
AGGAAAGTGGAGAATAATCA+AGG 0.493846 5_3:+14208905 None:intergenic
ACTCACGGAAATACTGATAG+TGG 0.495121 5_3:-14207173 Msa0800710:CDS
TGGGAATTGAAGTGAAGAAA+GGG 0.497041 5_3:+14207688 None:intergenic
AAAGCTGAAAATGAGATAAG+AGG 0.499953 5_3:+14207719 None:intergenic
GATAATGGAAGGAACGGAAT+TGG 0.500398 5_3:-14207560 Msa0800710:CDS
GTGTTTCCAGGCCAAATCAC+AGG 0.500547 5_3:+14206741 None:intergenic
GAAAATGGACAAGGCACTAA+AGG 0.505355 5_3:+14208885 None:intergenic
GATAATTGTCACTGTGAAAG+TGG 0.508548 5_3:+14209142 None:intergenic
TGATGGGCACTGGTTATGTA+TGG 0.510763 5_3:-14207346 Msa0800710:CDS
TATGAAATCATCAATGTGGT+TGG 0.513116 5_3:-14206879 Msa0800710:CDS
ATGGGAATTGAAGTGAAGAA+AGG 0.515929 5_3:+14207687 None:intergenic
AATTCGGATCCAAATATTCT+GGG 0.517215 5_3:-14208213 Msa0800710:CDS
AAGAAAGAGTTTAAGCATTG+TGG 0.517701 5_3:+14208861 None:intergenic
GTTAACATGACAGGTGTGAC+AGG 0.520717 5_3:-14206945 Msa0800710:CDS
CAATGGCTTCTCTTCAACTG+TGG 0.525200 5_3:-14208352 Msa0800710:CDS
GGAACCGGGTCTCGGAGGAT+CGG 0.535259 5_3:-14206858 Msa0800710:CDS
GGTTATTGGTCTAATCACTC+TGG 0.537000 5_3:-14206837 Msa0800710:CDS
CTTTCAATACTAGTGTTGGT+AGG 0.538929 5_3:-14208275 Msa0800710:CDS
CAAACCAGGCAATAACTTCA+GGG 0.551751 5_3:-14206781 Msa0800710:CDS
GCGAAGAATCTTGGAATGAT+GGG 0.551860 5_3:-14207362 Msa0800710:CDS
TCCATTGCCTTCTGATGCAA+TGG 0.552390 5_3:-14207282 Msa0800710:CDS
GACAAGGCACTAAAGGAAAG+TGG 0.558982 5_3:+14208892 None:intergenic
ACCTTGTTGACTACTATGGA+TGG 0.559074 5_3:-14207613 Msa0800710:CDS
ATTTATGAAGTTAACATGAC+AGG 0.559107 5_3:-14206954 Msa0800710:CDS
AAATTATTTGTACGGACAAG+AGG 0.562235 5_3:+14209056 None:intergenic
TCATCAATGTGGTTGGAACC+GGG 0.562645 5_3:-14206872 Msa0800710:CDS
AATCTTGGAATGATGGGCAC+TGG 0.563222 5_3:-14207356 Msa0800710:CDS
GGAAAGTGGAGAATAATCAA+GGG 0.563377 5_3:+14208906 None:intergenic
GATGATGATAATGGAAGGAA+CGG 0.567888 5_3:-14207566 Msa0800710:CDS
TAATAGCCAAACTTTAACCA+TGG 0.573491 5_3:+14208392 None:intergenic
GATGCATTGAACATATTCGA+TGG 0.575341 5_3:-14206999 Msa0800710:CDS
AATAGCAGAAAGAAAAGCAG+TGG 0.580483 5_3:+14207319 None:intergenic
AACAAATGTGCTAGTTCAGG+TGG 0.591116 5_3:-14207453 Msa0800710:CDS
GCTCTAATGATTCTCTACAA+TGG 0.596281 5_3:-14208369 Msa0800710:CDS
ATGAAGAAGGAACCAAAAGT+AGG 0.597085 5_3:+14208834 None:intergenic
AAATGGCAAAACTTGACTCA+CGG 0.603058 5_3:-14207188 Msa0800710:CDS
TCCATTGCATCAGAAGGCAA+TGG 0.606203 5_3:+14207281 None:intergenic
ATATCATCCATTGCATCAGA+AGG 0.609534 5_3:+14207275 None:intergenic
GAAACACAAACATCTTGTGA+TGG 0.609914 5_3:+14209199 None:intergenic
ATTGAAGTGAAGAAAGGGTA+GGG 0.610945 5_3:+14207693 None:intergenic
AATTATTTGTACGGACAAGA+GGG 0.612764 5_3:+14209057 None:intergenic
AGTTCACGCCAACACTATTG+GGG 0.619321 5_3:-14207405 Msa0800710:CDS
ATAGGTAAATCATACAACCA+TGG 0.621935 5_3:-14208409 Msa0800710:intron
ATAACCGATCCTCCGAGACC+CGG 0.622653 5_3:+14206854 None:intergenic
AATTGAAGTGAAGAAAGGGT+AGG 0.623429 5_3:+14207692 None:intergenic
TGTTTCCAGGCCAAATCACA+GGG 0.631591 5_3:+14206742 None:intergenic
ACATTGATGATGATAATGGA+AGG 0.631961 5_3:-14207571 Msa0800710:CDS
TGAGCTGTTACAGAACTATG+AGG 0.636099 5_3:+14207776 None:intergenic
TTTCATATGTAGGATTAACA+AGG 0.639032 5_3:+14206895 None:intergenic
CATTGTGGAGAAAATGGACA+AGG 0.642314 5_3:+14208876 None:intergenic
TCCATCCATAGTAGTCAACA+AGG 0.646917 5_3:+14207612 None:intergenic
GATGCAATGGATGATATCCA+AGG 0.647811 5_3:-14207269 Msa0800710:CDS
ATGGAGGAAACACATTGCGT+AGG 0.650471 5_3:-14206980 Msa0800710:CDS
GCCAAAATATCCAACTTCTG+AGG 0.651090 5_3:-14209010 Msa0800710:five_prime_UTR
CATACCTTAGAGCTTCAGGA+AGG 0.662211 5_3:+14208773 None:intergenic
GAGACCCGAAGCAACTGCAG+AGG 0.663454 5_3:-14207480 Msa0800710:CDS
TACATATGAAATCATCAATG+TGG 0.671358 5_3:-14206883 Msa0800710:CDS
GCATTGAACATATTCGATGG+AGG 0.674675 5_3:-14206996 Msa0800710:CDS
TGGTTGGAACCGGGTCTCGG+AGG 0.677436 5_3:-14206863 Msa0800710:CDS
GAAACACTTAATTCCAAACC+AGG 0.686145 5_3:-14206795 Msa0800710:CDS
GTTCACGCCAACACTATTGG+GGG 0.693725 5_3:-14207404 Msa0800710:CDS
GGTCATGGCTAGACACAATG+TGG 0.699792 5_3:-14207810 Msa0800710:CDS
TTGGCACTCATAATTCAACA+AGG 0.717953 5_3:-14208329 Msa0800710:CDS
AACACAGCTCAGAAGCCTCG+CGG 0.732843 5_3:-14206723 Msa0800710:intron
TCAAAGTTATCTACACTACG+TGG 0.734230 5_3:-14207038 Msa0800710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAGTCAAATTTTTTTA+TGG + chr5_3:14207846-14207865 None:intergenic 10.0%
!!! TTTTCTTCAAATTATTTGTA+CGG + chr5_3:14206745-14206764 None:intergenic 15.0%
!!! AAAGTTTTTTTTACTCTATA+TGG + chr5_3:14206844-14206863 None:intergenic 15.0%
!!! AAAATTATCATTGTTTTACA+AGG + chr5_3:14207712-14207731 None:intergenic 15.0%
!!! TTTTTTATTAGCAGATACTT+TGG - chr5_3:14207130-14207149 Msa0800710:CDS 20.0%
!!! CAAATTTTTTTATGGTCTAA+CGG + chr5_3:14207838-14207857 None:intergenic 20.0%
!!! ATAAGTGTAAATTTTTTGCA+CGG + chr5_3:14207910-14207929 None:intergenic 20.0%
!!! AAAGAAAGTTTGTTTCTAAA+TGG - chr5_3:14208585-14208604 Msa0800710:intron 20.0%
!!! TTGATGCATTTTTTAAACAA+GGG - chr5_3:14208705-14208724 Msa0800710:intron 20.0%
!! TGAAAACATGTGAGAAATTT+TGG + chr5_3:14206688-14206707 None:intergenic 25.0%
! AAGAAAAAAAGACAAGAAGT+CGG - chr5_3:14206758-14206777 Msa0800710:CDS 25.0%
!! CTATATGGTGTTGAAAAATA+TGG + chr5_3:14206829-14206848 None:intergenic 25.0%
!! TTTTGCATGAACAAAGATTT+TGG + chr5_3:14207256-14207275 None:intergenic 25.0%
!!! ATGCAAAATCTTGTTTTTCT+TGG - chr5_3:14207268-14207287 Msa0800710:CDS 25.0%
!!! CTCTATGTCTATTGTTTTAT+AGG - chr5_3:14207363-14207382 Msa0800710:CDS 25.0%
!! TTTTCTTTCAATACTAGTGT+TGG - chr5_3:14207511-14207530 Msa0800710:CDS 25.0%
!! TATGTAATATACAGTTTTGC+AGG - chr5_3:14207959-14207978 Msa0800710:intron 25.0%
!!! GTATACATTGATGATGATAA+TGG - chr5_3:14208215-14208234 Msa0800710:CDS 25.0%
!!! CTTGATGCATTTTTTAAACA+AGG - chr5_3:14208704-14208723 Msa0800710:intron 25.0%
! AATCATTTGAGAATGTGATT+TGG + chr5_3:14208733-14208752 None:intergenic 25.0%
! ATTTATGAAGTTAACATGAC+AGG - chr5_3:14208836-14208855 Msa0800710:five_prime_UTR 25.0%
! TTTCATATGTAGGATTAACA+AGG + chr5_3:14208898-14208917 None:intergenic 25.0%
!! ACATTGATGATTTCATATGT+AGG + chr5_3:14208908-14208927 None:intergenic 25.0%
! TACATATGAAATCATCAATG+TGG - chr5_3:14208907-14208926 Msa0800710:five_prime_UTR 25.0%
! GTTTCATTATTTCTTGGCTT+TGG + chr5_3:14206566-14206585 None:intergenic 30.0%
! GTGTGAGTTTCATTATTTCT+TGG + chr5_3:14206572-14206591 None:intergenic 30.0%
! AATTTCTCACATGTTTTCAG+TGG - chr5_3:14206689-14206708 Msa0800710:intron 30.0%
AATTATTTGTACGGACAAGA+GGG + chr5_3:14206736-14206755 None:intergenic 30.0%
AAATTATTTGTACGGACAAG+AGG + chr5_3:14206737-14206756 None:intergenic 30.0%
! TTTAAGCATTGTGGAGAAAA+TGG + chr5_3:14206923-14206942 None:intergenic 30.0%
! AAGAAAGAGTTTAAGCATTG+TGG + chr5_3:14206932-14206951 None:intergenic 30.0%
! TCTTTCTTTGTTCCTACTTT+TGG - chr5_3:14206944-14206963 Msa0800710:CDS 30.0%
CTTAGAATTGTGAATGAAGA+AGG + chr5_3:14206972-14206991 None:intergenic 30.0%
! TTTTTGTCCTTTCACATGTT+AGG - chr5_3:14207076-14207095 Msa0800710:CDS 30.0%
! TTTTGTCCTTTCACATGTTA+GGG - chr5_3:14207077-14207096 Msa0800710:CDS 30.0%
! GCATAAGATAAAGCAGATTT+TGG + chr5_3:14207169-14207188 None:intergenic 30.0%
CATAACAACAACAAAGCAAA+GGG + chr5_3:14207336-14207355 None:intergenic 30.0%
ACATAACAACAACAAAGCAA+AGG + chr5_3:14207337-14207356 None:intergenic 30.0%
ATAGGTAAATCATACAACCA+TGG - chr5_3:14207381-14207400 Msa0800710:CDS 30.0%
ATACAACCATGGTTAAAGTT+TGG - chr5_3:14207392-14207411 Msa0800710:CDS 30.0%
TAATAGCCAAACTTTAACCA+TGG + chr5_3:14207401-14207420 None:intergenic 30.0%
AGGCCTGATATTGTAAATAT+TGG - chr5_3:14207481-14207500 Msa0800710:CDS 30.0%
GCTCCAATATTTACAATATC+AGG + chr5_3:14207487-14207506 None:intergenic 30.0%
!! AAAAATTGCTTTAGAAGCTG+CGG - chr5_3:14207543-14207562 Msa0800710:CDS 30.0%
AAATTCGGATCCAAATATTC+TGG - chr5_3:14207576-14207595 Msa0800710:CDS 30.0%
AATTCGGATCCAAATATTCT+GGG - chr5_3:14207577-14207596 Msa0800710:CDS 30.0%
CAAGAAGATTCTAAGTATAG+AGG - chr5_3:14207625-14207644 Msa0800710:CDS 30.0%
TGACAGTGTATAATCTGAAA+AGG + chr5_3:14207780-14207799 None:intergenic 30.0%
AAAGCTGAAAATGAGATAAG+AGG + chr5_3:14208074-14208093 None:intergenic 30.0%
! GACAAGTTCTGATAAAGAAT+GGG + chr5_3:14208124-14208143 None:intergenic 30.0%
! TGACAAGTTCTGATAAAGAA+TGG + chr5_3:14208125-14208144 None:intergenic 30.0%
TCACAGTGATCTTTATCAAA+TGG - chr5_3:14208142-14208161 Msa0800710:intron 30.0%
!! ACATTGATGATGATAATGGA+AGG - chr5_3:14208219-14208238 Msa0800710:CDS 30.0%
ACAAAAAACTAAGTCCAAGA+GGG + chr5_3:14208643-14208662 None:intergenic 30.0%
! GGATTAACAAGGTTTTTATC+AGG + chr5_3:14208887-14208906 None:intergenic 30.0%
TATGAAATCATCAATGTGGT+TGG - chr5_3:14208911-14208930 Msa0800710:five_prime_UTR 30.0%
!! ATTAAGTGTTTCTGGAGAAA+CGG + chr5_3:14208987-14209006 None:intergenic 30.0%
GAAACACAAACATCTTGTGA+TGG + chr5_3:14206594-14206613 None:intergenic 35.0%
GATAATTGTCACTGTGAAAG+TGG + chr5_3:14206651-14206670 None:intergenic 35.0%
! TCCTCAGAAGTTGGATATTT+TGG + chr5_3:14206784-14206803 None:intergenic 35.0%
GGAAAGTGGAGAATAATCAA+GGG + chr5_3:14206887-14206906 None:intergenic 35.0%
AGGAAAGTGGAGAATAATCA+AGG + chr5_3:14206888-14206907 None:intergenic 35.0%
ATGAAGAAGGAACCAAAAGT+AGG + chr5_3:14206959-14206978 None:intergenic 35.0%
CATTCACAATTCTAAGCTAC+TGG - chr5_3:14206976-14206995 Msa0800710:CDS 35.0%
CAAAATCCCTAACATGTGAA+AGG + chr5_3:14207086-14207105 None:intergenic 35.0%
!!! CTTGGTGCTTTATGACTTTT+TGG - chr5_3:14207286-14207305 Msa0800710:CDS 35.0%
GCTCTAATGATTCTCTACAA+TGG - chr5_3:14207421-14207440 Msa0800710:CDS 35.0%
TTGGCACTCATAATTCAACA+AGG - chr5_3:14207461-14207480 Msa0800710:CDS 35.0%
!! CTTTCAATACTAGTGTTGGT+AGG - chr5_3:14207515-14207534 Msa0800710:CDS 35.0%
! TTGAAGAGAGAGCTTTAACT+TGG + chr5_3:14207608-14207627 None:intergenic 35.0%
! ATATACAGTTTTGCAGGTCA+TGG - chr5_3:14207965-14207984 Msa0800710:intron 35.0%
AGACACAATGTGGCAATAAT+TGG - chr5_3:14207990-14208009 Msa0800710:intron 35.0%
ATTGAAGTGAAGAAAGGGTA+GGG + chr5_3:14208100-14208119 None:intergenic 35.0%
AATTGAAGTGAAGAAAGGGT+AGG + chr5_3:14208101-14208120 None:intergenic 35.0%
TGGGAATTGAAGTGAAGAAA+GGG + chr5_3:14208105-14208124 None:intergenic 35.0%
ATGGGAATTGAAGTGAAGAA+AGG + chr5_3:14208106-14208125 None:intergenic 35.0%
!! GATGATGATAATGGAAGGAA+CGG - chr5_3:14208224-14208243 Msa0800710:CDS 35.0%
GATAACAAATGTGCTAGTTC+AGG - chr5_3:14208334-14208353 Msa0800710:CDS 35.0%
AATAGCAGAAAGAAAAGCAG+TGG + chr5_3:14208474-14208493 None:intergenic 35.0%
ATATCATCCATTGCATCAGA+AGG + chr5_3:14208518-14208537 None:intergenic 35.0%
! TTTCTTTTAAGCTCCGAATC+TGG + chr5_3:14208572-14208591 None:intergenic 35.0%
AAATGGCAAAACTTGACTCA+CGG - chr5_3:14208602-14208621 Msa0800710:intron 35.0%
GACAAAAAACTAAGTCCAAG+AGG + chr5_3:14208644-14208663 None:intergenic 35.0%
TCAAAGTTATCTACACTACG+TGG - chr5_3:14208752-14208771 Msa0800710:intron 35.0%
GATGCATTGAACATATTCGA+TGG - chr5_3:14208791-14208810 Msa0800710:five_prime_UTR 35.0%
! GGTTTGGAATTAAGTGTTTC+TGG + chr5_3:14208995-14209014 None:intergenic 35.0%
GAAACACTTAATTCCAAACC+AGG - chr5_3:14208995-14209014 Msa0800710:five_prime_UTR 35.0%
AAATGCTATTCCCTGTGATT+TGG - chr5_3:14209038-14209057 Msa0800710:five_prime_UTR 35.0%
GCCAAAATATCCAACTTCTG+AGG - chr5_3:14206780-14206799 Msa0800710:CDS 40.0%
! GAAAATGGACAAGGCACTAA+AGG + chr5_3:14206908-14206927 None:intergenic 40.0%
CATTGTGGAGAAAATGGACA+AGG + chr5_3:14206917-14206936 None:intergenic 40.0%
ATATCCTTCCTGAAGCTCTA+AGG - chr5_3:14207013-14207032 Msa0800710:CDS 40.0%
AAGACATACCTTAGAGCTTC+AGG + chr5_3:14207024-14207043 None:intergenic 40.0%
TGCGGTGAACGATGTAAATT+CGG - chr5_3:14207561-14207580 Msa0800710:CDS 40.0%
!! AGGTTTTCTGAGTATTGCTG+AGG - chr5_3:14207645-14207664 Msa0800710:CDS 40.0%
!! GGTTTTCTGAGTATTGCTGA+GGG - chr5_3:14207646-14207665 Msa0800710:CDS 40.0%
GGTTCATCAGCTCATGAAAA+GGG + chr5_3:14207691-14207710 None:intergenic 40.0%
AGGTTCATCAGCTCATGAAA+AGG + chr5_3:14207692-14207711 None:intergenic 40.0%
TGAGCTGTTACAGAACTATG+AGG + chr5_3:14208017-14208036 None:intergenic 40.0%
! ACCTTGTTGACTACTATGGA+TGG - chr5_3:14208177-14208196 Msa0800710:CDS 40.0%
TCCATCCATAGTAGTCAACA+AGG + chr5_3:14208181-14208200 None:intergenic 40.0%
!! GATAATGGAAGGAACGGAAT+TGG - chr5_3:14208230-14208249 Msa0800710:CDS 40.0%
AACAAATGTGCTAGTTCAGG+TGG - chr5_3:14208337-14208356 Msa0800710:CDS 40.0%
TAGTTCACGCCAACACTATT+GGG - chr5_3:14208384-14208403 Msa0800710:CDS 40.0%
TTCGCGACACTAAACACTTT+CGG + chr5_3:14208413-14208432 None:intergenic 40.0%
TTTAGTGTCGCGAAGAATCT+TGG - chr5_3:14208419-14208438 Msa0800710:intron 40.0%
GCGAAGAATCTTGGAATGAT+GGG - chr5_3:14208428-14208447 Msa0800710:intron 40.0%
GATGCAATGGATGATATCCA+AGG - chr5_3:14208521-14208540 Msa0800710:intron 40.0%
ACTCACGGAAATACTGATAG+TGG - chr5_3:14208617-14208636 Msa0800710:intron 40.0%
GCATTGAACATATTCGATGG+AGG - chr5_3:14208794-14208813 Msa0800710:five_prime_UTR 40.0%
ATCATCAATGTGGTTGGAAC+CGG - chr5_3:14208917-14208936 Msa0800710:five_prime_UTR 40.0%
! GGTTATTGGTCTAATCACTC+TGG - chr5_3:14208953-14208972 Msa0800710:five_prime_UTR 40.0%
CAAACCAGGCAATAACTTCA+GGG - chr5_3:14209009-14209028 Msa0800710:five_prime_UTR 40.0%
CAATCACACTCCTCAGAAGT+TGG + chr5_3:14206793-14206812 None:intergenic 45.0%
! CAACTTCTGAGGAGTGTGAT+TGG - chr5_3:14206791-14206810 Msa0800710:CDS 45.0%
! GACAAGGCACTAAAGGAAAG+TGG + chr5_3:14206901-14206920 None:intergenic 45.0%
CATACCTTAGAGCTTCAGGA+AGG + chr5_3:14207020-14207039 None:intergenic 45.0%
CAATGGCTTCTCTTCAACTG+TGG - chr5_3:14207438-14207457 Msa0800710:CDS 45.0%
TTGGTCTCGCCCAGAATATT+TGG + chr5_3:14207589-14207608 None:intergenic 45.0%
! GCAGACCTTGTTGACTACTA+TGG - chr5_3:14208173-14208192 Msa0800710:CDS 45.0%
AGGAACGGAATTGGTGCATT+AGG - chr5_3:14208239-14208258 Msa0800710:CDS 45.0%
TGAGATTCTGCAACGCTTCT+CGG + chr5_3:14208276-14208295 None:intergenic 45.0%
TATCTCCTCTGCAGTTGCTT+CGG + chr5_3:14208318-14208337 None:intergenic 45.0%
GTAGTTCACGCCAACACTAT+TGG - chr5_3:14208383-14208402 Msa0800710:CDS 45.0%
AGTTCACGCCAACACTATTG+GGG - chr5_3:14208385-14208404 Msa0800710:CDS 45.0%
TCGCGACACTAAACACTTTC+GGG + chr5_3:14208412-14208431 None:intergenic 45.0%
CGCGAAGAATCTTGGAATGA+TGG - chr5_3:14208427-14208446 Msa0800710:intron 45.0%
!! AATCTTGGAATGATGGGCAC+TGG - chr5_3:14208434-14208453 Msa0800710:intron 45.0%
!! TGATGGGCACTGGTTATGTA+TGG - chr5_3:14208444-14208463 Msa0800710:intron 45.0%
TCCATTGCCTTCTGATGCAA+TGG - chr5_3:14208508-14208527 Msa0800710:intron 45.0%
TCCATTGCATCAGAAGGCAA+TGG + chr5_3:14208512-14208531 None:intergenic 45.0%
!! AATACTGATAGTGGCCCTCT+TGG - chr5_3:14208626-14208645 Msa0800710:intron 45.0%
ATGGAGGAAACACATTGCGT+AGG - chr5_3:14208810-14208829 Msa0800710:five_prime_UTR 45.0%
GTTAACATGACAGGTGTGAC+AGG - chr5_3:14208845-14208864 Msa0800710:five_prime_UTR 45.0%
TCATCAATGTGGTTGGAACC+GGG - chr5_3:14208918-14208937 Msa0800710:five_prime_UTR 45.0%
CCTGAAGTTATTGCCTGGTT+TGG + chr5_3:14209011-14209030 None:intergenic 45.0%
CCAAACCAGGCAATAACTTC+AGG - chr5_3:14209008-14209027 Msa0800710:five_prime_UTR 45.0%
TGTTTCCAGGCCAAATCACA+GGG + chr5_3:14209051-14209070 None:intergenic 45.0%
!!! ATGATAATTTTTTTTTTTTT+TGG - chr5_3:14207721-14207740 Msa0800710:CDS 5.0%
GTACGGACAAGAGGGACAAT+TGG + chr5_3:14206728-14206747 None:intergenic 50.0%
GGCTTCTCTTCAACTGTGGT+TGG - chr5_3:14207442-14207461 Msa0800710:CDS 50.0%
GGTCATGGCTAGACACAATG+TGG - chr5_3:14207980-14207999 Msa0800710:intron 50.0%
ATCTCCTCTGCAGTTGCTTC+GGG + chr5_3:14208317-14208336 None:intergenic 50.0%
GTTCACGCCAACACTATTGG+GGG - chr5_3:14208386-14208405 Msa0800710:CDS 50.0%
CTCTCCCTGAAGTTATTGCC+TGG + chr5_3:14209016-14209035 None:intergenic 50.0%
! CTATTCCCTGTGATTTGGCC+TGG - chr5_3:14209043-14209062 Msa0800710:five_prime_UTR 50.0%
GTGTTTCCAGGCCAAATCAC+AGG + chr5_3:14209052-14209071 None:intergenic 50.0%
TTTCGGGCCCCCAATAGTGT+TGG + chr5_3:14208396-14208415 None:intergenic 55.0%
ATGTGGTTGGAACCGGGTCT+CGG - chr5_3:14208924-14208943 Msa0800710:five_prime_UTR 55.0%
ATAACCGATCCTCCGAGACC+CGG + chr5_3:14208939-14208958 None:intergenic 55.0%
GGTCTCGGAGGATCGGTTAT+TGG - chr5_3:14208939-14208958 Msa0800710:five_prime_UTR 55.0%
GGCTTCTGAGCTGTGTTTCC+AGG + chr5_3:14209064-14209083 None:intergenic 55.0%
AACACAGCTCAGAAGCCTCG+CGG - chr5_3:14209067-14209086 Msa0800710:five_prime_UTR 55.0%
TTCGGAAAAACCCAACCGCG+AGG + chr5_3:14209085-14209104 None:intergenic 55.0%
GCAGTTGCTTCGGGTCTCAC+AGG + chr5_3:14208308-14208327 None:intergenic 60.0%
GAGACCCGAAGCAACTGCAG+AGG - chr5_3:14208310-14208329 Msa0800710:CDS 60.0%
CGCGTGCTGTCAACACTCCT+TGG + chr5_3:14208541-14208560 None:intergenic 60.0%
ACGCGTGCACACGCCAGATT+CGG - chr5_3:14208556-14208575 Msa0800710:intron 60.0%
AGCTCAGAAGCCTCGCGGTT+GGG - chr5_3:14209072-14209091 Msa0800710:five_prime_UTR 60.0%
TGGTTGGAACCGGGTCTCGG+AGG - chr5_3:14208927-14208946 Msa0800710:five_prime_UTR 65.0%
GGAACCGGGTCTCGGAGGAT+CGG - chr5_3:14208932-14208951 Msa0800710:five_prime_UTR 65.0%
CAGCTCAGAAGCCTCGCGGT+TGG - chr5_3:14209071-14209090 Msa0800710:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 14206552 14209260 14206552 ID=Msa0800710;Name=Msa0800710
chr5_3 mRNA 14206552 14209260 14206552 ID=Msa0800710-mRNA-1;Parent=Msa0800710;Name=Msa0800710-mRNA-1;_AED=0.02;_eAED=0.02;_QI=498|0.66|0.5|0.75|0.66|0.5|4|0|472
chr5_3 exon 14206552 14206586 14206552 ID=Msa0800710-mRNA-1:exon:5994;Parent=Msa0800710-mRNA-1
chr5_3 exon 14206724 14207839 14206724 ID=Msa0800710-mRNA-1:exon:5993;Parent=Msa0800710-mRNA-1
chr5_3 exon 14208145 14208427 14208145 ID=Msa0800710-mRNA-1:exon:5992;Parent=Msa0800710-mRNA-1
chr5_3 exon 14208778 14209260 14208778 ID=Msa0800710-mRNA-1:exon:5991;Parent=Msa0800710-mRNA-1
chr5_3 five_prime_UTR 14208778 14209260 14208778 ID=Msa0800710-mRNA-1:five_prime_utr;Parent=Msa0800710-mRNA-1
chr5_3 five_prime_UTR 14208413 14208427 14208413 ID=Msa0800710-mRNA-1:five_prime_utr;Parent=Msa0800710-mRNA-1
chr5_3 CDS 14208145 14208412 14208145 ID=Msa0800710-mRNA-1:cds;Parent=Msa0800710-mRNA-1
chr5_3 CDS 14206724 14207839 14206724 ID=Msa0800710-mRNA-1:cds;Parent=Msa0800710-mRNA-1
chr5_3 CDS 14206552 14206586 14206552 ID=Msa0800710-mRNA-1:cds;Parent=Msa0800710-mRNA-1
Gene Sequence

>Msa0800710

ATGGTTAAAGTTTGGCTATTAGCTCTAATGATTCTCTACAATGGCTTCTCTTCAACTGTGGTTGGCACTCATAATTCAACAAGGCCTGATATTGTAAATATTGGAGCTCTTTTTTCTTTCAATACTAGTGTTGGTAGGATTATAAAAATTGCTTTAGAAGCTGCGGTGAACGATGTAAATTCGGATCCAAATATTCTGGGCGAGACCAAGTTAAAGCTCTCTCTTCAAGAAGATTCTAAGTATAGAGGTTTTCTGAGTATTGCTGAGGTTTTGCAGGTCATGGCTAGACACAATGTGGCAATAATTGGTCCTCATAGTTCTGTAACAGCTCATGTCATAACTCATATTGCAAATGAGCTTCAAGTTCCTCTTATCTCATTTTCAGCTTTAGACCCTACCCTTTCTTCACTTCAATTCCCATTCTTTATCAGAACTTGTCACAGTGATCTTTATCAAATGGCTGCAATAGCAGACCTTGTTGACTACTATGGATGGAAAGAAGTCATTGCTGTATACATTGATGATGATAATGGAAGGAACGGAATTGGTGCATTAGGCGATAAGCTTGCCGAGAAGCGTTGCAGAATCTCATATAAAGCGCCTGTGAGACCCGAAGCAACTGCAGAGGAGATAACAAATGTGCTAGTTCAGGTGGCTCTTGCTGAATCAAGAGTTATAGTAGTTCACGCCAACACTATTGGGGGCCCGAAAGTGTTTAGTGTCGCGAAGAATCTTGGAATGATGGGCACTGGTTATGTATGGATAGCCACTGCTTTTCTTTCTGCTATTCTTGATATAAATAGTCCATTGCCTTCTGATGCAATGGATGATATCCAAGGAGTGTTGACAGCACGCGTGCACACGCCAGATTCGGAGCTTAAAAGAAAGTTTGTTTCTAAATGGCAAAACTTGACTCACGGAAATACTGATAGTGGCCCTCTTGGACTTAGTTTTTTGTCTCTCTATGCATATGATACTATTTATGCTCTTGCTCATGCACTTGATGCATTTTTTAAACAAGGGAACCAAATCACATTCTCAAATGATTCAAAGTTATCTACACTACGTGGAGACAACTTGCATCTTGATGCATTGAACATATTCGATGGAGGAAACACATTGCGTAGGAACATTTATGAAGTTAACATGACAGGTGTGACAGGCTTGTTCAAGTATGCACCTGATAAAAACCTTGTTAATCCTACATATGAAATCATCAATGTGGTTGGAACCGGGTCTCGGAGGATCGGTTATTGGTCTAATCACTCTGGATTATCATCCGTTTCTCCAGAAACACTTAATTCCAAACCAGGCAATAACTTCAGGGAGAGTAAAATGCTATTCCCTGTGATTTGGCCTGGAAACACAGCTCAGAAGCCTCGCGAAGCATATACTTATACTTATAATAAACATTTATAG
Protein sequence

>Msa0800710

MVKVWLLALMILYNGFSSTVVGTHNSTRPDIVNIGALFSFNTSVGRIIKIALEAAVNDVNSDPNILGETKLKLSLQEDSKYRGFLSIAEVLQVMARHNVAIIGPHSSVTAHVITHIANELQVPLISFSALDPTLSSLQFPFFIRTCHSDLYQMAAIADLVDYYGWKEVIAVYIDDDNGRNGIGALGDKLAEKRCRISYKAPVRPEATAEEITNVLVQVALAESRVIVVHANTIGGPKVFSVAKNLGMMGTGYVWIATAFLSAILDINSPLPSDAMDDIQGVLTARVHTPDSELKRKFVSKWQNLTHGNTDSGPLGLSFLSLYAYDTIYALAHALDAFFKQGNQITFSNDSKLSTLRGDNLHLDALNIFDGGNTLRRNIYEVNMTGVTGLFKYAPDKNLVNPTYEIINVVGTGSRRIGYWSNHSGLSSVSPETLNSKPGNNFRESKMLFPVIWPGNTAQKPREAYTYTYNKHL*